GID8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GID8
Entrez Gene ID	54994
Full Name	GID complex subunit 8 homolog
Synonyms	C20orf11,TWA1
General protein information	Preferred Names GID complex subunit 8 homolog Names glucose-induced degradation protein 8 homolog protein C20orf11 two hybrid associated protein No. 1 with RanBPM two hybrid-associated protein 1 with RanBPM
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q13.33
Disorder MIM:	611625

mRNA and Protein(s)

mRNA	Protein	Name
NM_017896.2	NP_060366.1	glucose-induced degradation protein 8 homolog
NM_017896.3	NP_060366.1	glucose-induced degradation protein 8 homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	GID8	NP_060366.1
Bos taurus (cattle)	GID8	NP_001032676.1
Mus musculus (house mouse)	Gid8	NP_083883.1
Rattus norvegicus (Norway rat)	Gid8	NP_942038.1
Danio rerio (zebrafish)	gid8b	XP_005162154.1
Arabidopsis thaliana (thale cress)	AT1G61150	NP_176310.3
Danio rerio (zebrafish)	gid8a	NP_957006.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000951	XP_001688166.1
	GID8	XP_001086462.1
Canis lupus familiaris (dog)	GID8	XP_545179.2
Drosophila melanogaster (fruit fly)	CG6617	NP_573315.1
Pan troglodytes (chimpanzee)	GID8	XP_001147288.1
Gallus gallus (chicken)	C20H20ORF11	NP_001007872.1
Oryza sativa (rice)	Os03g0235100	NP_001049484.1
Xenopus tropicalis (tropical clawed frog)	gid8	XP_002944197.1

Gene Ontology
Bibliography

Related articles in PubMed

Twa1/Gid8 is a ?-catenin nuclear retention factor in Wnt signaling and colorectal tumorigenesis.
Lu Y, Xie S, Zhang W, Zhang C, Gao C, Sun Q, Cai Y, Xu Z, Xiao M, Xu Y, Huang X, Wu X, Liu W, Wang F, Kang Y, Zhou T
Cell research27(12)1422-1440(2017 Dec)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Boldt K, van Reeuwijk J, Lu Q, Koutroumpas K, Nguyen TM, Texier Y, van Beersum SE, Horn N, Willer JR, Mans DA, Dougherty G, Lamers IJ, Coene KL, Arts HH, Betts MJ, Beyer T, Bolat E, Gloeckner CJ, Haidari K, Hetterschijt L, Iaconis D, Jenkins D, Klose F, Knapp B, Latour B, Letteboer SJ, Marcelis CL, Mitic D, Morleo M, Oud MM, Riemersma M, Rix S, Terhal PA, Toedt G, van Dam TJ, de Vrieze E, Wissinger Y, Wu KM, Apic G, Beales PL, Blacque OE, Gibson TJ, Huynen MA, Katsanis N, Kremer H, Omran H, van Wijk E, Wolfrum U, Kepes F, Davis EE, Franco B, Giles RH, Ueffing M, Russell RB, Roepman R,
Nature communications711491(2016 May)

Molecular phylogeny of a RING E3 ubiquitin ligase, conserved in eukaryotic cells and dominated by homologous components, the muskelin/RanBPM/CTLH complex.
Francis O, Han F, Adams JC
PloS one8(10)e75217(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GID8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GID8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29335	NM_017896.3 Latest version!	Homo sapiens GID complex subunit 8 homolog (GID8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu29335	NM_017896.2	Homo sapiens GID complex subunit 8 homolog (GID8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GID8 ( NM_017896.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060366.1 Homo sapiens GID complex subunit 8 homolog (GID8), mRNA.

Price & Availability↑

CloneID	OHu29335
Clone ID Related Accession (Same CDS sequence)	NM_017896.2 , NM_017896.3
Accession Version	NM_017896.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	glucose-induced degradation protein 8 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CD676269.1, AK000609.1, BC032120.2, BQ931571.1, BQ935273.1, AK025775.1 and BC031819.1. On or before Aug 31, 2013 this sequence version replaced XM_005260220.1, NM_017896.1. ##Evidence-Data-START## Transcript exon combination :: BX349966.2, SRR1803617.31349.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTATG CAGAAAAACC CGATGAAATC ACGAAAGATG AGTGGATGGA AAAGCTCAAT 
AACTTGCATG TCCAGAGAGC AGACATGAAC CGCCTCATCA TGAACTACCT GGTCACAGAG 
GGCTTTAAGG AAGCAGCGGA GAAGTTTCGA ATGGAATCTG GAATCGAACC TAGTGTGGAT 
CTGGAAACAC TTGATGAACG AATCAAGATC CGGGAGATGA TACTGAAAGG TCAGATTCAG 
GAGGCCATCG CCTTGATCAA CAGCCTCCAC CCAGAGCTCT TGGACACAAA CCGGTATCTT 
TACTTCCATT TGCAGCAACA GCATTTGATC GAGCTGATCC GCCAGCGGGA GACAGAGGCG 
GCGCTGGAGT TTGCACAGAC TCAGCTGGCG GAGCAGGGCG AGGAGAGCCG AGAGTGCCTC 
ACAGAGATGG AGCGTACCCT GGCACTGCTG GCCTTTGACA GTCCCGAGGA GTCGCCCTTC 
GGAGACCTCC TCCACACCAT GCAGAGGCAG AAGGTGTGGA GTGAAGTTAA CCAAGCTGTG 
CTAGATTATG AAAATCGCGA GTCAACACCC AAACTGGCAA AATTACTGAA ACTACTACTT 
TGGGCTCAGA ACGAGCTGGA CCAGAAGAAA GTAAAATATC CCAAAATGAC AGACCTCAGC 
AAGGGTGTGA TTGAGGAGCC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060366.1
CDS	178..864
Translation	MSYAEKPDEITKDEWMEKLNNLHVQRADMNRLIMNYLVTEGFKEAAEKFRMESGIEPSVDLETLDERIKIREMILKGQIQEAIALINSLHPELLDTNRYLYFHLQQQHLIELIRQRETEAALEFAQTQLAEQGEESRECLTEMERTLALLAFDSPEESPFGDLLHTMQRQKVWSEVNQAVLDYENRESTPKLAKLLKLLLWAQNELDQKKVKYPKMTDLSKGVIEEPK

Target ORF information:

RefSeq Version	NM_017896.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GID complex subunit 8 homolog (GID8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017896.2

ORF Insert Sequence:

ATGAGTTATG CAGAAAAACC CGATGAAATC ACGAAAGATG AGTGGATGGA AAAGCTCAAT 
AACTTGCATG TCCAGAGAGC AGACATGAAC CGCCTCATCA TGAACTACCT GGTCACAGAG 
GGCTTTAAGG AAGCAGCGGA GAAGTTTCGA ATGGAATCTG GAATCGAACC TAGTGTGGAT 
CTGGAAACAC TTGATGAACG AATCAAGATC CGGGAGATGA TACTGAAAGG TCAGATTCAG 
GAGGCCATCG CCTTGATCAA CAGCCTCCAC CCAGAGCTCT TGGACACAAA CCGGTATCTT 
TACTTCCATT TGCAGCAACA GCATTTGATC GAGCTGATCC GCCAGCGGGA GACAGAGGCG 
GCGCTGGAGT TTGCACAGAC TCAGCTGGCG GAGCAGGGCG AGGAGAGCCG AGAGTGCCTC 
ACAGAGATGG AGCGTACCCT GGCACTGCTG GCCTTTGACA GTCCCGAGGA GTCGCCCTTC 
GGAGACCTCC TCCACACCAT GCAGAGGCAG AAGGTGTGGA GTGAAGTTAA CCAAGCTGTG 
CTAGATTATG AAAATCGCGA GTCAACACCC AAACTGGCAA AATTACTGAA ACTACTACTT 
TGGGCTCAGA ACGAGCTGGA CCAGAAGAAA GTAAAATATC CCAAAATGAC AGACCTCAGC 
AAGGGTGTGA TTGAGGAGCC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GID8 ( NM_017896.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060366.1 Homo sapiens GID complex subunit 8 homolog (GID8), mRNA.

Price & Availability↑

CloneID	OHu29335
Clone ID Related Accession (Same CDS sequence)	NM_017896.2 , NM_017896.3
Accession Version	NM_017896.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	glucose-induced degradation protein 8 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CD676269.1, AK000609.1, BC032120.2, BQ931571.1, BQ935273.1, AK025775.1 and BC031819.1. On Nov 23, 2018 this sequence version replaced NM_017896.2. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.251224.1, SRR1803611.275507.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000266069.5/ ENSP00000266069.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTTATG CAGAAAAACC CGATGAAATC ACGAAAGATG AGTGGATGGA AAAGCTCAAT 
AACTTGCATG TCCAGAGAGC AGACATGAAC CGCCTCATCA TGAACTACCT GGTCACAGAG 
GGCTTTAAGG AAGCAGCGGA GAAGTTTCGA ATGGAATCTG GAATCGAACC TAGTGTGGAT 
CTGGAAACAC TTGATGAACG AATCAAGATC CGGGAGATGA TACTGAAAGG TCAGATTCAG 
GAGGCCATCG CCTTGATCAA CAGCCTCCAC CCAGAGCTCT TGGACACAAA CCGGTATCTT 
TACTTCCATT TGCAGCAACA GCATTTGATC GAGCTGATCC GCCAGCGGGA GACAGAGGCG 
GCGCTGGAGT TTGCACAGAC TCAGCTGGCG GAGCAGGGCG AGGAGAGCCG AGAGTGCCTC 
ACAGAGATGG AGCGTACCCT GGCACTGCTG GCCTTTGACA GTCCCGAGGA GTCGCCCTTC 
GGAGACCTCC TCCACACCAT GCAGAGGCAG AAGGTGTGGA GTGAAGTTAA CCAAGCTGTG 
CTAGATTATG AAAATCGCGA GTCAACACCC AAACTGGCAA AATTACTGAA ACTACTACTT 
TGGGCTCAGA ACGAGCTGGA CCAGAAGAAA GTAAAATATC CCAAAATGAC AGACCTCAGC 
AAGGGTGTGA TTGAGGAGCC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060366.1
CDS	120..806
Translation	MSYAEKPDEITKDEWMEKLNNLHVQRADMNRLIMNYLVTEGFKEAAEKFRMESGIEPSVDLETLDERIKIREMILKGQIQEAIALINSLHPELLDTNRYLYFHLQQQHLIELIRQRETEAALEFAQTQLAEQGEESRECLTEMERTLALLAFDSPEESPFGDLLHTMQRQKVWSEVNQAVLDYENRESTPKLAKLLKLLLWAQNELDQKKVKYPKMTDLSKGVIEEPK

Target ORF information:

RefSeq Version	NM_017896.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GID complex subunit 8 homolog (GID8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017896.3

ORF Insert Sequence:

ATGAGTTATG CAGAAAAACC CGATGAAATC ACGAAAGATG AGTGGATGGA AAAGCTCAAT 
AACTTGCATG TCCAGAGAGC AGACATGAAC CGCCTCATCA TGAACTACCT GGTCACAGAG 
GGCTTTAAGG AAGCAGCGGA GAAGTTTCGA ATGGAATCTG GAATCGAACC TAGTGTGGAT 
CTGGAAACAC TTGATGAACG AATCAAGATC CGGGAGATGA TACTGAAAGG TCAGATTCAG 
GAGGCCATCG CCTTGATCAA CAGCCTCCAC CCAGAGCTCT TGGACACAAA CCGGTATCTT 
TACTTCCATT TGCAGCAACA GCATTTGATC GAGCTGATCC GCCAGCGGGA GACAGAGGCG 
GCGCTGGAGT TTGCACAGAC TCAGCTGGCG GAGCAGGGCG AGGAGAGCCG AGAGTGCCTC 
ACAGAGATGG AGCGTACCCT GGCACTGCTG GCCTTTGACA GTCCCGAGGA GTCGCCCTTC 
GGAGACCTCC TCCACACCAT GCAGAGGCAG AAGGTGTGGA GTGAAGTTAA CCAAGCTGTG 
CTAGATTATG AAAATCGCGA GTCAACACCC AAACTGGCAA AATTACTGAA ACTACTACTT 
TGGGCTCAGA ACGAGCTGGA CCAGAAGAAA GTAAAATATC CCAAAATGAC AGACCTCAGC 
AAGGGTGTGA TTGAGGAGCC CAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Twa1/Gid8 is a ?-catenin nuclear retention factor in Wnt signaling and colorectal tumorigenesis.
Cell research27(12)1422-1440(2017 Dec)
Lu Y,Xie S,Zhang W,Zhang C,Gao C,Sun Q,Cai Y,Xu Z,Xiao M,Xu Y,Huang X,Wu X,Liu W,Wang F,Kang Y,Zhou T

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Nature communications711491(2016 May)
Boldt K,van Reeuwijk J,Lu Q,Koutroumpas K,Nguyen TM,Texier Y,van Beersum SE,Horn N,Willer JR,Mans DA,Dougherty G,Lamers IJ,Coene KL,Arts HH,Betts MJ,Beyer T,Bolat E,Gloeckner CJ,Haidari K,Hetterschijt L,Iaconis D,Jenkins D,Klose F,Knapp B,Latour B,Letteboer SJ,Marcelis CL,Mitic D,Morleo M,Oud MM,Riemersma M,Rix S,Terhal PA,Toedt G,van Dam TJ,de Vrieze E,Wissinger Y,Wu KM,Apic G,Beales PL,Blacque OE,Gibson TJ,Huynen MA,Katsanis N,Kremer H,Omran H,van Wijk E,Wolfrum U,Kepes F,Davis EE,Franco B,Giles RH,Ueffing M,Russell RB,Roepman R,

Molecular phylogeny of a RING E3 ubiquitin ligase, conserved in eukaryotic cells and dominated by homologous components, the muskelin/RanBPM/CTLH complex.
PloS one8(10)e75217(2013)
Francis O,Han F,Adams JC