PFKM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PFKM
Entrez Gene ID	5213
Full Name	phosphofructokinase, muscle
Synonyms	ATP-PFK,GSD7,PFK-1,PFK-A,PFK1,PFKA,PFKX,PPP1R122
General protein information	Preferred Names phosphofructokinase, muscle Names ATP-dependent 6-phosphofructokinase, muscle type 6-phosphofructo-1-kinase 6-phosphofructokinase type A 6-phosphofructokinase, muscle type phosphofructo-1-kinase isozyme A phosphofructokinase 1 phosphofructokinase, polypeptide X phosphofructokinase-M phosphohexokinase protein phosphatase 1, regulatory subunit 122
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.11
Summary	Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009].
Disorder MIM:	610681
Disorder Html:	Glycogen storage disease VII, 232800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005268977.1	XP_005269034.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X3
NM_000289.5	NP_000280.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
NM_001354739.1	NP_001341668.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 1
NM_001354738.1	NP_001341667.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 1
XM_005268976.3	XP_005269033.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_011538487.1	XP_011536789.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
NM_001354743.1	NP_001341672.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
NM_001354737.1	NP_001341666.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 1
NM_001354736.1	NP_001341665.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 3
NM_001354748.1	NP_001341677.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 8
NM_001354742.1	NP_001341671.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
NM_001354744.1	NP_001341673.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
NM_001166687.1	NP_001160159.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
NM_001354741.1	NP_001341670.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 5
XM_017019469.1	XP_016874958.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X5
XM_024449022.1	XP_024304790.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X7
XM_005268978.2	XP_005269035.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X3
XM_005268975.1	XP_005269032.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_011538488.1	XP_011536790.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
NM_001166686.1	NP_001160158.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 1
NM_001363619.1	NP_001350548.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 9
NM_001354740.1	NP_001341669.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 4
NM_001354745.1	NP_001341674.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 6
XM_024449020.1	XP_024304788.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X3
XM_005268979.1	XP_005269036.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X4
XM_024449021.1	XP_024304789.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X6
XM_017019468.1	XP_016874957.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X3
NM_001354735.1	NP_001341664.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 3
NM_001166688.1	NP_001160160.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
XM_005268974.1	XP_005269031.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_017019467.1	XP_016874956.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
XM_011538488.2	XP_011536790.1	ATP-dependent 6-phosphofructokinase, muscle type isoform X5
NM_001354747.1	NP_001341676.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 8
NM_001354746.1	NP_001341675.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 7
NM_000289.6	NP_000280.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
NM_001166686.2	NP_001160158.1	ATP-dependent 6-phosphofructokinase, muscle type isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PFKM	NP_001160158.1
Rattus norvegicus (Norway rat)	Pfkm	NP_113903.1
Mus musculus (house mouse)	Pfkm	NP_067489.3
Xenopus tropicalis (tropical clawed frog)	pfkm	XP_004911968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007642	XP_564589.3
Pan troglodytes (chimpanzee)	PFKM	XP_509029.2
	PFKM	XP_001096244.1
Canis lupus familiaris (dog)	PFKM	NP_001003199.1
Bos taurus (cattle)	PFKM	NP_001068736.1
Drosophila melanogaster (fruit fly)	Pfk	NP_724891.2
Caenorhabditis elegans (roundworm)	Y71H10A.1	NP_741738.1
Gallus gallus (chicken)	PFKM	NP_989554.2
Danio rerio (zebrafish)	pfkmb	NP_001082886.1
Danio rerio (zebrafish)	pfkma	NP_001004575.1

Gene Ontology
Bibliography

Related articles in PubMed

The glycolytic enzyme phosphofructokinase-1 assembles into filaments.
Webb BA, Dosey AM, Wittmann T, Kollman JM, Barber DL
The Journal of cell biology216(8)2305-2313(2017 Aug)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

The expression pattern of PFKFB3 enzyme distinguishes between induced-pluripotent stem cells and cancer stem cells.
Cie?lar-Pobuda A, Jain MV, Kratz G, Rzeszowska-Wolny J, Ghavami S, Wiechec E
Oncotarget6(30)29753-70(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase3 (PFKFB3) and phosphofructokinase (PFK1) expression allows discrimination between induced pluripotent stem cells (iPS) and cancer stem cells (CSCs).
Title: The expression pattern of PFKFB3 enzyme distinguishes between induced-pluripotent stem cells and cancer stem cells.

characterize three PFK1 mutations and show they have distinct effects on allosteric regulation of PFKP activity and lactate production.
Title: Structures of human phosphofructokinase-1 and atomic basis of cancer-associated mutations.

Gene-based analysis identified an association between breast cancer and the phosphofructokinase-muscle (PFKM) gene on chromosome 12q13.11 that met the genome-wide gene-based threshold of 2.5 x 10(-6).
Title: A genome-wide association study of early-onset breast cancer identifies PFKM as a novel breast cancer gene and supports a common genetic spectrum for breast cancer at any age.

insulin/phosphofructokinase (PFKM), overlaps with an expression quantitative trait loci
Title: Expression of phosphofructokinase in skeletal muscle is influenced by genetic variation and associated with insulin sensitivity.

Asp(543)Ala, a naturally occurring Tarui disease-causing mutation in the activating binding site, causes an increased efficacy of ATP at the inhibitory allosteric binding site.
Title: Altered allosteric regulation of muscle 6-phosphofructokinase causes Tarui disease.

The following PFKM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFKM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28127	NM_001166686.2 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu28128	NM_000289.6 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28128	NM_001166688.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28128	NM_001354743.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28128	NM_001354744.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28128	NM_001354742.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28128	NM_001166687.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28128	XM_024449022.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28128	XM_011538488.2 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu102907	NM_001354745.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OHu28127	NM_001354738.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu28127	NM_001354737.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu28127	NM_001354739.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu46638	NM_001354736.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu46638	NM_001354735.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu28127	XM_017019468.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu28127	XM_005268978.2 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu28127	XM_005268977.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu28127	XM_005268979.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu46638	XM_005268974.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu46638	XM_005268975.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu46638	XM_005268976.3 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu46638	XM_017019467.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu59637	XM_011538487.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu102861	NM_001354748.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu102861	NM_001354747.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu102886	NM_001354746.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu102915	NM_001354741.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu102933	NM_001354740.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu106940	NM_001363619.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu103737	XM_024449020.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu103738	XM_024449021.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu83887	XM_017019469.1 Latest version!	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu28128	XM_011538488.1	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28128	NM_000289.5	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu28127	NM_001166686.1	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PFKM ( NM_001166686.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001160158.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 1, mRNA.

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CloneID	OHu28127
Clone ID Related Accession (Same CDS sequence)	XM_005268977.1 , NM_001354739.1 , NM_001354738.1 , NM_001354737.1 , XM_005268978.2 , NM_001166686.1 , XM_005268979.1 , XM_017019468.1 , NM_001166686.2
Accession Version	NM_001166686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB081387.1, BC021203.2, BC000534.2 and BU683561.1. On Jul 12, 2018 this sequence version replaced NM_001166686.1. Transcript Variant: This variant (1) (also known as TE-PFKM) encodes isoform 1. Variants 1, 7, 8, and 9 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK126229.1, SRR1803613.90467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001160158.1
CDS	225..2780
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001166686.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166686.2

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_000289.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000280.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu28128
Clone ID Related Accession (Same CDS sequence)	NM_000289.5 , NM_001354743.1 , NM_001354742.1 , NM_001354744.1 , NM_001166687.1 , XM_024449022.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2 , NM_000289.6
Accession Version	NM_000289.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA525437.1, BC021203.2, BC000534.2 and BU683561.1. This sequence is a reference standard in the RefSeqGene project. On Nov 11, 2009 this sequence version replaced NM_000289.4. Transcript Variant: This variant (4, also known as type C) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3, 4, 12, 13, and 14 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.88852.1, SRR1803612.128307.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000280.1
CDS	186..2528
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_000289.5
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000289.5

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354743.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341672.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 13, mRNA.

Price & Availability↑

CloneID	OHu28128
Clone ID Related Accession (Same CDS sequence)	NM_000289.5 , NM_001354743.1 , NM_001354742.1 , NM_001354744.1 , NM_001166687.1 , XM_024449022.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2 , NM_000289.6
Accession Version	NM_001354743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.239809.1, SRR1803611.147961.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341672.1
CDS	199..2541
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354743.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354743.1

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354742.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341671.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 12, mRNA.

Price & Availability↑

CloneID	OHu28128
Clone ID Related Accession (Same CDS sequence)	NM_000289.5 , NM_001354743.1 , NM_001354742.1 , NM_001354744.1 , NM_001166687.1 , XM_024449022.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2 , NM_000289.6
Accession Version	NM_001354742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. On Aug 29, 2017 this sequence version replaced XM_011538488.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.106822.1, SRR1660805.113607.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341671.1
CDS	176..2518
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354742.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354742.1

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354744.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341673.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 14, mRNA.

Price & Availability↑

CloneID	OHu28128
Clone ID Related Accession (Same CDS sequence)	NM_000289.5 , NM_001354743.1 , NM_001354742.1 , NM_001354744.1 , NM_001166687.1 , XM_024449022.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2 , NM_000289.6
Accession Version	NM_001354744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.26390.1, SRR1660805.101431.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341673.1
CDS	234..2576
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354744.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354744.1

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001166687.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001160159.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28128
Clone ID Related Accession (Same CDS sequence)	NM_000289.5 , NM_001354743.1 , NM_001354742.1 , NM_001354744.1 , NM_001166687.1 , XM_024449022.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2 , NM_000289.6
Accession Version	NM_001166687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA560355.1, Y00698.1, BC000534.2 and BU683561.1. Transcript Variant: This variant (2, also known as type A) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3, 4, 12, 13, and 14 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y00698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001160159.1
CDS	40..2382
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001166687.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166687.1

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001166688.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001160160.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28128
Clone ID Related Accession (Same CDS sequence)	NM_000289.5 , NM_001354743.1 , NM_001354742.1 , NM_001354744.1 , NM_001166687.1 , XM_024449022.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2 , NM_000289.6
Accession Version	NM_001166688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA761651.1, BC000534.2 and BU683561.1. Transcript Variant: This variant (3, also known as type B) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3, 4, 12, 13, and 14 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.91353.1, BC000534.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001160160.1
CDS	129..2471
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001166688.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166688.1

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_000289.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_000280.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu28128
Clone ID Related Accession (Same CDS sequence)	NM_000289.5 , NM_001354743.1 , NM_001354742.1 , NM_001354744.1 , NM_001166687.1 , XM_024449022.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2 , NM_000289.6
Accession Version	NM_000289.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA525437.1, BC021203.2, BC000534.2 and BU683561.1. This sequence is a reference standard in the RefSeqGene project. On Jul 6, 2018 this sequence version replaced NM_000289.5. Transcript Variant: This variant (4, also known as type C) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3, 4, 12, 13, and 14 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.163458.1, SRR1660805.57430.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000359794.11/ ENSP00000352842.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000280.1
CDS	52..2394
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_000289.6
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000289.6

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_024449022.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304790.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu28128
Clone ID Related Accession (Same CDS sequence)	NM_000289.5 , NM_001354743.1 , NM_001354742.1 , NM_001354744.1 , NM_001166687.1 , XM_024449022.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2 , NM_000289.6
Accession Version	XM_024449022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304790.1
CDS	152..2494
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_024449022.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449022.1

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_011538488.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536790.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu28128
Clone ID Related Accession (Same CDS sequence)	NM_000289.5 , NM_001354743.1 , NM_001354742.1 , NM_001354744.1 , NM_001166687.1 , XM_024449022.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2 , NM_000289.6
Accession Version	XM_011538488.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
Comment

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011536790.1

Translation

MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_011538488.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538488.1

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_011538488.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536790.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu28128
Clone ID Related Accession (Same CDS sequence)	NM_000289.5 , NM_001354743.1 , NM_001354742.1 , NM_001354744.1 , NM_001166687.1 , XM_024449022.1 , XM_011538488.1 , NM_001166688.1 , XM_011538488.2 , NM_000289.6
Accession Version	XM_011538488.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536790.1
CDS	151..2493
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_011538488.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538488.2

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354745.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341674.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 15, mRNA.

Price & Availability↑

CloneID	OHu102907
Clone ID Related Accession (Same CDS sequence)	NM_001354745.1
Accession Version	NM_001354745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.249841.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341674.1
CDS	511..2766
Translation	MNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354745.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354745.1

ORF Insert Sequence:

ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354739.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341668.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu28127
Clone ID Related Accession (Same CDS sequence)	XM_005268977.1 , NM_001354739.1 , NM_001354738.1 , NM_001354737.1 , XM_005268978.2 , NM_001166686.1 , XM_005268979.1 , XM_017019468.1 , NM_001166686.2
Accession Version	NM_001354739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. On Aug 24, 2017 this sequence version replaced XM_017019468.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.92512.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341668.1
CDS	147..2702
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354739.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354739.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354738.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341667.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu28127
Clone ID Related Accession (Same CDS sequence)	XM_005268977.1 , NM_001354739.1 , NM_001354738.1 , NM_001354737.1 , XM_005268978.2 , NM_001166686.1 , XM_005268979.1 , XM_017019468.1 , NM_001166686.2
Accession Version	NM_001354738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. On Aug 24, 2017 this sequence version replaced XM_005268978.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.113293.1, SRR1660803.245434.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341667.1
CDS	76..2631
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354738.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354738.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354737.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341666.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 7, mRNA.

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CloneID	OHu28127
Clone ID Related Accession (Same CDS sequence)	XM_005268977.1 , NM_001354739.1 , NM_001354738.1 , NM_001354737.1 , XM_005268978.2 , NM_001166686.1 , XM_005268979.1 , XM_017019468.1 , NM_001166686.2
Accession Version	NM_001354737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. On Aug 29, 2017 this sequence version replaced XM_005268977.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.11935.1, SRR1660805.66755.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341666.1
CDS	206..2761
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354737.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354737.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001166686.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001160158.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28127
Clone ID Related Accession (Same CDS sequence)	XM_005268977.1 , NM_001354739.1 , NM_001354738.1 , NM_001354737.1 , XM_005268978.2 , NM_001166686.1 , XM_005268979.1 , XM_017019468.1 , NM_001166686.2
Accession Version	NM_001166686.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB081387.1, BC021203.2, BC000534.2 and BU683561.1. Transcript Variant: This variant (1) (also known as TE-PFKM) encodes isoform 1. Variants 1, 7, 8, and 9 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK126229.1, SRR1803613.90467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001160158.1
CDS	275..2830
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001166686.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166686.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354736.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341665.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu46638
Clone ID Related Accession (Same CDS sequence)	XM_005268976.3 , NM_001354736.1 , XM_005268975.1 , NM_001354735.1 , XM_005268974.1 , XM_017019467.1
Accession Version	NM_001354736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. On Sep 13, 2017 this sequence version replaced XM_005268975.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.250028.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG 
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT 
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC 
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT 
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC 
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG 
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT 
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT 
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG 
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC 
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG 
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG 
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC 
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG 
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT 
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG 
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA 
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT 
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT 
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT 
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT 
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT 
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC 
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT 
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA 
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341665.1
CDS	206..2857
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTECAEVEIQVSKRKRAVVKARGDPTVETMKQREEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354736.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354736.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG 
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT 
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC 
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT 
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC 
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG 
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT 
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT 
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG 
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC 
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG 
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG 
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC 
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG 
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT 
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG 
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA 
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT 
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT 
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT 
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT 
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT 
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC 
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT 
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA 
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354735.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341664.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu46638
Clone ID Related Accession (Same CDS sequence)	XM_005268976.3 , NM_001354736.1 , XM_005268975.1 , NM_001354735.1 , XM_005268974.1 , XM_017019467.1
Accession Version	NM_001354735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. On Aug 24, 2017 this sequence version replaced XM_017019467.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.52383.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG 
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT 
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC 
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT 
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC 
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG 
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT 
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT 
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG 
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC 
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG 
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG 
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC 
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG 
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT 
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG 
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA 
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT 
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT 
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT 
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT 
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT 
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC 
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT 
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA 
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341664.1
CDS	147..2798
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTECAEVEIQVSKRKRAVVKARGDPTVETMKQREEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354735.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354735.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG 
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT 
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC 
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT 
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC 
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG 
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT 
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT 
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG 
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC 
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG 
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG 
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC 
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG 
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT 
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG 
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA 
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT 
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT 
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT 
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT 
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT 
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC 
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT 
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA 
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_005268977.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269034.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu28127
Clone ID Related Accession (Same CDS sequence)	XM_005268977.1 , NM_001354739.1 , NM_001354738.1 , NM_001354737.1 , XM_005268978.2 , NM_001166686.1 , XM_005268979.1 , XM_017019468.1 , NM_001166686.2
Accession Version	XM_005268977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269034.1
CDS	158..2713
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_005268977.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268977.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_005268978.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269035.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu28127
Clone ID Related Accession (Same CDS sequence)	XM_005268977.1 , NM_001354739.1 , NM_001354738.1 , NM_001354737.1 , XM_005268978.2 , NM_001166686.1 , XM_005268979.1 , XM_017019468.1 , NM_001166686.2
Accession Version	XM_005268978.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005268978.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269035.1
CDS	71..2626
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_005268978.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268978.2

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_005268979.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269036.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X5, mRNA.

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CloneID	OHu28127
Clone ID Related Accession (Same CDS sequence)	XM_005268977.1 , NM_001354739.1 , NM_001354738.1 , NM_001354737.1 , XM_005268978.2 , NM_001166686.1 , XM_005268979.1 , XM_017019468.1 , NM_001166686.2
Accession Version	XM_005268979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269036.1
CDS	158..2713
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_005268979.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268979.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_017019468.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874957.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu28127
Clone ID Related Accession (Same CDS sequence)	XM_005268977.1 , NM_001354739.1 , NM_001354738.1 , NM_001354737.1 , XM_005268978.2 , NM_001166686.1 , XM_005268979.1 , XM_017019468.1 , NM_001166686.2
Accession Version	XM_017019468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874957.1
CDS	93..2648
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_017019468.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019468.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATCTGG TGGTTAAGCG TCTGGGATAT 
GACACCCGGG TTACTGTCTT GGGGCATGTG CAGAGGGGTG GGACGCCATC AGCCTTTGAC 
AGAATTCTGG GCAGCAGGAT GGGTGTGGAA GCAGTGATGG CACTTTTGGA GGGGACCCCA 
GATACCCCAG CCTGTGTAGT GAGCCTCTCT GGTAACCAGG CTGTGCGCCT GCCCCTCATG 
GAATGTGTCC AGGTGACCAA AGATGTGACC AAGGCCATGG ATGAGAAGAA ATTTGACGAA 
GCCCTGAAGC TGAGAGGCCG GAGCTTCATG AACAACTGGG AGGTGTACAA GCTTCTAGCT 
CATGTCAGAC CCCCGGTATC TAAGAGTGGT TCGCACACAG TGGCTGTGAT GAACGTGGGG 
GCTCCGGCTG CAGGCATGAA TGCTGCTGTT CGCTCCACTG TGAGGATTGG CCTTATCCAG 
GGCAACCGAG TGCTCGTTGT CCATGATGGT TTCGAGGGCC TGGCCAAGGG GCAGATAGAG 
GAAGCTGGCT GGAGCTATGT TGGGGGCTGG ACTGGCCAAG GTGGCTCTAA ACTTGGGACT 
AAAAGGACTC TACCCAAGAA GAGCTTTGAA CAGATCAGTG CCAATATAAC TAAGTTTAAC 
ATTCAGGGCC TTGTCATCAT TGGGGGCTTT GAGGCTTACA CAGGGGGCCT GGAACTGATG 
GAGGGCAGGA AGCAGTTTGA TGAGCTCTGC ATCCCATTTG TGGTCATTCC TGCTACAGTC 
TCCAACAATG TCCCTGGCTC AGACTTCAGC GTTGGGGCTG ACACAGCACT CAATACTATC 
TGCACAACCT GTGACCGCAT CAAGCAGTCA GCAGCTGGCA CCAAGCGTCG GGTGTTTATC 
ATTGAGACTA TGGGTGGCTA CTGTGGCTAC CTGGCTACCA TGGCTGGACT GGCAGCTGGG 
GCCGATGCTG CCTACATTTT TGAGGAGCCC TTCACCATTC GAGACCTGCA GGCAAATGTT 
GAACATCTGG TGCAAAAGAT GAAAACAACT GTGAAAAGGG GCTTGGTGTT AAGGAATGAA 
AAGTGCAATG AGAACTATAC CACTGACTTC ATTTTCAACC TGTACTCTGA GGAGGGGAAG 
GGCATCTTCG ACAGCAGGAA GAATGTGCTT GGTCACATGC AGCAGGGTGG GAGCCCAACC 
CCATTTGATA GGAATTTTGC CACTAAGATG GGCGCCAAGG CTATGAACTG GATGTCTGGG 
AAAATCAAAG AGAGTTACCG TAATGGGCGG ATCTTTGCCA ATACTCCAGA TTCGGGCTGT 
GTTCTGGGGA TGCGTAAGAG GGCTCTGGTC TTCCAACCAG TGGCTGAGCT GAAGGACCAG 
ACAGATTTTG AGCATCGAAT CCCCAAGGAA CAGTGGTGGC TGAAACTGAG GCCCATCCTC 
AAAATCCTAG CCAAGTACGA GATTGACTTG GACACTTCAG ACCATGCCCA CCTGGAGCAC 
ATCACCCGGA AGCGGTCCGG GGAAGCTGCC GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_005268976.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269033.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu46638
Clone ID Related Accession (Same CDS sequence)	XM_005268976.3 , NM_001354736.1 , XM_005268975.1 , NM_001354735.1 , XM_005268974.1 , XM_017019467.1
Accession Version	XM_005268976.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268976.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG 
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT 
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC 
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT 
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC 
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG 
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT 
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT 
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG 
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC 
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG 
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG 
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC 
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG 
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT 
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG 
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA 
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT 
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT 
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT 
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT 
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT 
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC 
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT 
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA 
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269033.1
CDS	258..2909
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTECAEVEIQVSKRKRAVVKARGDPTVETMKQREEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_005268976.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268976.3

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG 
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT 
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC 
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT 
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC 
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG 
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT 
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT 
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG 
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC 
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG 
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG 
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC 
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG 
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT 
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG 
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA 
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT 
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT 
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT 
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT 
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT 
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC 
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT 
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA 
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_005268975.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269032.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu46638
Clone ID Related Accession (Same CDS sequence)	XM_005268976.3 , NM_001354736.1 , XM_005268975.1 , NM_001354735.1 , XM_005268974.1 , XM_017019467.1
Accession Version	XM_005268975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG 
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT 
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC 
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT 
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC 
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG 
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT 
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT 
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG 
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC 
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG 
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG 
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC 
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG 
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT 
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG 
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA 
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT 
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT 
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT 
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT 
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT 
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC 
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT 
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA 
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269032.1
CDS	215..2866
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTECAEVEIQVSKRKRAVVKARGDPTVETMKQREEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_005268975.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268975.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG 
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT 
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC 
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT 
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC 
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG 
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT 
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT 
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG 
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC 
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG 
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG 
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC 
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG 
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT 
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG 
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA 
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT 
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT 
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT 
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT 
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT 
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC 
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT 
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA 
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_005268974.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269031.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu46638
Clone ID Related Accession (Same CDS sequence)	XM_005268976.3 , NM_001354736.1 , XM_005268975.1 , NM_001354735.1 , XM_005268974.1 , XM_017019467.1
Accession Version	XM_005268974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG 
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT 
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC 
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT 
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC 
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG 
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT 
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT 
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG 
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC 
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG 
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG 
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC 
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG 
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT 
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG 
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA 
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT 
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT 
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT 
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT 
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT 
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC 
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT 
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA 
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269031.1
CDS	76..2727
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTECAEVEIQVSKRKRAVVKARGDPTVETMKQREEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_005268974.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268974.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG 
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT 
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC 
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT 
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC 
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG 
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT 
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT 
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG 
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC 
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG 
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG 
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC 
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG 
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT 
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG 
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA 
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT 
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT 
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT 
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT 
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT 
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC 
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT 
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA 
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_017019467.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874956.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu46638
Clone ID Related Accession (Same CDS sequence)	XM_005268976.3 , NM_001354736.1 , XM_005268975.1 , NM_001354735.1 , XM_005268974.1 , XM_017019467.1
Accession Version	XM_017019467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG 
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT 
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC 
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT 
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC 
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG 
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT 
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT 
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG 
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC 
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG 
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG 
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC 
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG 
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT 
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG 
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA 
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT 
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT 
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT 
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT 
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT 
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC 
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT 
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA 
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874956.1
CDS	145..2796
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTECAEVEIQVSKRKRAVVKARGDPTVETMKQREEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_017019467.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019467.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATCTGGTGGT TAAGCGTCTG GGATATGACA CCCGGGTTAC TGTCTTGGGG 
CATGTGCAGA GGGGTGGGAC GCCATCAGCC TTTGACAGAA TTCTGGGCAG CAGGATGGGT 
GTGGAAGCAG TGATGGCACT TTTGGAGGGG ACCCCAGATA CCCCAGCCTG TGTAGTGAGC 
CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC CTCATGGAAT GTGTCCAGGT GACCAAAGAT 
GTGACCAAGG CCATGGATGA GAAGAAATTT GACGAAGCCC TGAAGCTGAG AGGCCGGAGC 
TTCATGAACA ACTGGGAGGT GTACAAGCTT CTAGCTCATG TCAGACCCCC GGTATCTAAG 
AGTGGTTCGC ACACAGTGGC TGTGATGAAC GTGGGGGCTC CGGCTGCAGG CATGAATGCT 
GCTGTTCGCT CCACTGTGAG GATTGGCCTT ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT 
GATGGTTTCG AGGGCCTGGC CAAGGGGCAG ATAGAGGAAG CTGGCTGGAG CTATGTTGGG 
GGCTGGACTG GCCAAGGTGG CTCTAAACTT GGGACTAAAA GGACTCTACC CAAGAAGAGC 
TTTGAACAGA TCAGTGCCAA TATAACTAAG TTTAACATTC AGGGCCTTGT CATCATTGGG 
GGCTTTGAGG CTTACACAGG GGGCCTGGAA CTGATGGAGG GCAGGAAGCA GTTTGATGAG 
CTCTGCATCC CATTTGTGGT CATTCCTGCT ACAGTCTCCA ACAATGTCCC TGGCTCAGAC 
TTCAGCGTTG GGGCTGACAC AGCACTCAAT ACTATCTGCA CAACCTGTGA CCGCATCAAG 
CAGTCAGCAG CTGGCACCAA GCGTCGGGTG TTTATCATTG AGACTATGGG TGGCTACTGT 
GGCTACCTGG CTACCATGGC TGGACTGGCA GCTGGGGCCG ATGCTGCCTA CATTTTTGAG 
GAGCCCTTCA CCATTCGAGA CCTGCAGGCA AATGTTGAAC ATCTGGTGCA AAAGATGAAA 
ACAACTGTGA AAAGGGGCTT GGTGTTAAGG AATGAAAAGT GCAATGAGAA CTATACCACT 
GACTTCATTT TCAACCTGTA CTCTGAGGAG GGGAAGGGCA TCTTCGACAG CAGGAAGAAT 
GTGCTTGGTC ACATGCAGCA GGGTGGGAGC CCAACCCCAT TTGATAGGAA TTTTGCCACT 
AAGATGGGCG CCAAGGCTAT GAACTGGATG TCTGGGAAAA TCAAAGAGAG TTACCGTAAT 
GGGCGGATCT TTGCCAATAC TCCAGATTCG GGCTGTGTTC TGGGGATGCG TAAGAGGGCT 
CTGGTCTTCC AACCAGTGGC TGAGCTGAAG GACCAGACAG ATTTTGAGCA TCGAATCCCC 
AAGGAACAGT GGTGGCTGAA ACTGAGGCCC ATCCTCAAAA TCCTAGCCAA GTACGAGATT 
GACTTGGACA CTTCAGACCA TGCCCACCTG GAGCACATCA CCCGGAAGCG GTCCGGGGAA 
GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_011538487.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536789.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X3, mRNA.

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CloneID	OHu59637
Clone ID Related Accession (Same CDS sequence)	XM_011538487.1
Accession Version	XM_011538487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATGGCAGCAG GATGGGTGTG GAAGCAGTGA TGGCACTTTT GGAGGGGACC 
CCAGATACCC CAGCCTGTGT AGTGAGCCTC TCTGGTAACC AGGCTGTGCG CCTGCCCCTC 
ATGGAATGTG TCCAGGTGAC CAAAGATGTG ACCAAGGCCA TGGATGAGAA GAAATTTGAC 
GAAGCCCTGA AGCTGAGAGG CCGGAGCTTC ATGAACAACT GGGAGGTGTA CAAGCTTCTA 
GCTCATGTCA GACCCCCGGT ATCTAAGAGT GGTTCGCACA CAGTGGCTGT GATGAACGTG 
GGGGCTCCGG CTGCAGGCAT GAATGCTGCT GTTCGCTCCA CTGTGAGGAT TGGCCTTATC 
CAGGGCAACC GAGTGCTCGT TGTCCATGAT GGTTTCGAGG GCCTGGCCAA GGGGCAGATA 
GAGGAAGCTG GCTGGAGCTA TGTTGGGGGC TGGACTGGCC AAGGTGGCTC TAAACTTGGG 
ACTAAAAGGA CTCTACCCAA GAAGAGCTTT GAACAGATCA GTGCCAATAT AACTAAGTTT 
AACATTCAGG GCCTTGTCAT CATTGGGGGC TTTGAGGCTT ACACAGGGGG CCTGGAACTG 
ATGGAGGGCA GGAAGCAGTT TGATGAGCTC TGCATCCCAT TTGTGGTCAT TCCTGCTACA 
GTCTCCAACA ATGTCCCTGG CTCAGACTTC AGCGTTGGGG CTGACACAGC ACTCAATACT 
ATCTGCACAA CCTGTGACCG CATCAAGCAG TCAGCAGCTG GCACCAAGCG TCGGGTGTTT 
ATCATTGAGA CTATGGGTGG CTACTGTGGC TACCTGGCTA CCATGGCTGG ACTGGCAGCT 
GGGGCCGATG CTGCCTACAT TTTTGAGGAG CCCTTCACCA TTCGAGACCT GCAGGCAAAT 
GTTGAACATC TGGTGCAAAA GATGAAAACA ACTGTGAAAA GGGGCTTGGT GTTAAGGAAT 
GAAAAGTGCA ATGAGAACTA TACCACTGAC TTCATTTTCA ACCTGTACTC TGAGGAGGGG 
AAGGGCATCT TCGACAGCAG GAAGAATGTG CTTGGTCACA TGCAGCAGGG TGGGAGCCCA 
ACCCCATTTG ATAGGAATTT TGCCACTAAG ATGGGCGCCA AGGCTATGAA CTGGATGTCT 
GGGAAAATCA AAGAGAGTTA CCGTAATGGG CGGATCTTTG CCAATACTCC AGATTCGGGC 
TGTGTTCTGG GGATGCGTAA GAGGGCTCTG GTCTTCCAAC CAGTGGCTGA GCTGAAGGAC 
CAGACAGATT TTGAGCATCG AATCCCCAAG GAACAGTGGT GGCTGAAACT GAGGCCCATC 
CTCAAAATCC TAGCCAAGTA CGAGATTGAC TTGGACACTT CAGACCATGC CCACCTGGAG 
CACATCACCC GGAAGCGGTC CGGGGAAGCT GCCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536789.1
CDS	10..2568
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTECAEVEIQVSKRKRAVVKARGDPTVETMKQREEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_011538487.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538487.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAATGT GCAGAGGTAG AGATACAAGT AAGCAAGAGG 
AAGAGGGCAG TGGTAAAAGC AAGAGGAGAC CCGACTGTGG AGACAATGAA GCAAAGGGAA 
GAGTGGATCA TGACCCATGA AGAGCACCAT GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC 
ATTGCTGTCT TAACCTCTGG TGGAGATGCC CAAGGTATGA ATGCTGCTGT CAGGGCTGTG 
GTTCGAGTTG GTATCTTCAC CGGTGCCCGT GTCTTCTTTG TCCATGAGGG TTATCAAGGC 
CTGGTGGATG GTGGAGATCA CATCAAGGAA GCCACCTGGG AGAGCGTTTC GATGATGCTT 
CAGCTGGGAG GCACGGTGAT TGGAAGTGCC CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA 
CGACTCCGAG CTGCCTACAA CCTGGTGAAG CGTGGGATCA CCAATCTCTG TGTCATTGGG 
GGTGATGGCA GCCTCACTGG GGCTGACACC TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT 
GACCTCCAGA AAGCAGGTAA GATCACAGAT GAGGAGGCTA CGAAGTCCAG CTACCTGAAC 
ATTGTGGGCC TGGTTGGGTC AATTGACAAT GACTTCTGTG GCACCGATAT GACCATTGGC 
ACTGACTCTG CCCTGCATCG GATCATGGAA ATTGTAGATG CCATCACTAC CACTGCCCAG 
AGCCACCAGA GGACATTTGT GTTAGAAGTA ATGGGCCGCC ACTGTGGATA CCTGGCCCTT 
GTCACCTCTC TGTCCTGTGG GGCCGACTGG GTTTTTATTC CTGAATGTCC ACCAGATGAC 
GACTGGGAGG AACACCTTTG TCGCCGACTC AGCGAGACAA GGACCCGTGG TTCTCGTCTC 
AACATCATCA TTGTGGCTGA GGGTGCAATT GACAAGAATG GAAAACCAAT CACCTCAGAA 
GACATCAAGA ATGGCAGCAG GATGGGTGTG GAAGCAGTGA TGGCACTTTT GGAGGGGACC 
CCAGATACCC CAGCCTGTGT AGTGAGCCTC TCTGGTAACC AGGCTGTGCG CCTGCCCCTC 
ATGGAATGTG TCCAGGTGAC CAAAGATGTG ACCAAGGCCA TGGATGAGAA GAAATTTGAC 
GAAGCCCTGA AGCTGAGAGG CCGGAGCTTC ATGAACAACT GGGAGGTGTA CAAGCTTCTA 
GCTCATGTCA GACCCCCGGT ATCTAAGAGT GGTTCGCACA CAGTGGCTGT GATGAACGTG 
GGGGCTCCGG CTGCAGGCAT GAATGCTGCT GTTCGCTCCA CTGTGAGGAT TGGCCTTATC 
CAGGGCAACC GAGTGCTCGT TGTCCATGAT GGTTTCGAGG GCCTGGCCAA GGGGCAGATA 
GAGGAAGCTG GCTGGAGCTA TGTTGGGGGC TGGACTGGCC AAGGTGGCTC TAAACTTGGG 
ACTAAAAGGA CTCTACCCAA GAAGAGCTTT GAACAGATCA GTGCCAATAT AACTAAGTTT 
AACATTCAGG GCCTTGTCAT CATTGGGGGC TTTGAGGCTT ACACAGGGGG CCTGGAACTG 
ATGGAGGGCA GGAAGCAGTT TGATGAGCTC TGCATCCCAT TTGTGGTCAT TCCTGCTACA 
GTCTCCAACA ATGTCCCTGG CTCAGACTTC AGCGTTGGGG CTGACACAGC ACTCAATACT 
ATCTGCACAA CCTGTGACCG CATCAAGCAG TCAGCAGCTG GCACCAAGCG TCGGGTGTTT 
ATCATTGAGA CTATGGGTGG CTACTGTGGC TACCTGGCTA CCATGGCTGG ACTGGCAGCT 
GGGGCCGATG CTGCCTACAT TTTTGAGGAG CCCTTCACCA TTCGAGACCT GCAGGCAAAT 
GTTGAACATC TGGTGCAAAA GATGAAAACA ACTGTGAAAA GGGGCTTGGT GTTAAGGAAT 
GAAAAGTGCA ATGAGAACTA TACCACTGAC TTCATTTTCA ACCTGTACTC TGAGGAGGGG 
AAGGGCATCT TCGACAGCAG GAAGAATGTG CTTGGTCACA TGCAGCAGGG TGGGAGCCCA 
ACCCCATTTG ATAGGAATTT TGCCACTAAG ATGGGCGCCA AGGCTATGAA CTGGATGTCT 
GGGAAAATCA AAGAGAGTTA CCGTAATGGG CGGATCTTTG CCAATACTCC AGATTCGGGC 
TGTGTTCTGG GGATGCGTAA GAGGGCTCTG GTCTTCCAAC CAGTGGCTGA GCTGAAGGAC 
CAGACAGATT TTGAGCATCG AATCCCCAAG GAACAGTGGT GGCTGAAACT GAGGCCCATC 
CTCAAAATCC TAGCCAAGTA CGAGATTGAC TTGGACACTT CAGACCATGC CCACCTGGAG 
CACATCACCC GGAAGCGGTC CGGGGAAGCT GCCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354748.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341677.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 18, mRNA.

Price & Availability↑

CloneID	OHu102861
Clone ID Related Accession (Same CDS sequence)	NM_001354748.1 , NM_001354747.1
Accession Version	NM_001354748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.40392.1, SRR1803611.39815.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCAAGG GTTATCAAGG CCTGGTGGAT GGTGGAGATC ACATCAAGGA AGCCACCTGG 
GAGAGCGTTT CGATGATGCT TCAGCTGGGA GGCACGGTGA TTGGAAGTGC CCGGTGCAAG 
GACTTTCGGG AACGAGAAGG ACGACTCCGA GCTGCCTACA ACCTGGTGAA GCGTGGGATC 
ACCAATCTCT GTGTCATTGG GGGTGATGGC AGCCTCACTG GGGCTGACAC CTTCCGTTCT 
GAGTGGAGTG ACTTGTTGAG TGACCTCCAG AAAGCAGGTA AGATCACAGA TGAGGAGGCT 
ACGAAGTCCA GCTACCTGAA CATTGTGGGC CTGGTTGGGT CAATTGACAA TGACTTCTGT 
GGCACCGATA TGACCATTGG CACTGACTCT GCCCTGCATC GGATCATGGA AATTGTAGAT 
GCCATCACTA CCACTGCCCA GAGCCACCAG AGGACATTTG TGTTAGAAGT AATGGGCCGC 
CACTGTGGAT ACCTGGCCCT TGTCACCTCT CTGTCCTGTG GGGCCGACTG GGTTTTTATT 
CCTGAATGTC CACCAGATGA CGACTGGGAG GAACACCTTT GTCGCCGACT CAGCGAGACA 
AGGACCCGTG GTTCTCGTCT CAACATCATC ATTGTGGCTG AGGGTGCAAT TGACAAGAAT 
GGAAAACCAA TCACCTCAGA AGACATCAAG AATCTGGTGG TTAAGCGTCT GGGATATGAC 
ACCCGGGTTA CTGTCTTGGG GCATGTGCAG AGGGGTGGGA CGCCATCAGC CTTTGACAGA 
ATTCTGGGCA GCAGGATGGG TGTGGAAGCA GTGATGGCAC TTTTGGAGGG GACCCCAGAT 
ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT AACCAGGCTG TGCGCCTGCC CCTCATGGAA 
TGTGTCCAGG TGACCAAAGA TGTGACCAAG GCCATGGATG AGAAGAAATT TGACGAAGCC 
CTGAAGCTGA GAGGCCGGAG CTTCATGAAC AACTGGGAGG TGTACAAGCT TCTAGCTCAT 
GTCAGACCCC CGGTATCTAA GAGTGGTTCG CACACAGTGG CTGTGATGAA CGTGGGGGCT 
CCGGCTGCAG GCATGAATGC TGCTGTTCGC TCCACTGTGA GGATTGGCCT TATCCAGGGC 
AACCGAGTGC TCGTTGTCCA TGATGGTTTC GAGGGCCTGG CCAAGGGGCA GATAGAGGAA 
GCTGGCTGGA GCTATGTTGG GGGCTGGACT GGCCAAGGTG GCTCTAAACT TGGGACTAAA 
AGGACTCTAC CCAAGAAGAG CTTTGAACAG ATCAGTGCCA ATATAACTAA GTTTAACATT 
CAGGGCCTTG TCATCATTGG GGGCTTTGAG GCTTACACAG GGGGCCTGGA ACTGATGGAG 
GGCAGGAAGC AGTTTGATGA GCTCTGCATC CCATTTGTGG TCATTCCTGC TACAGTCTCC 
AACAATGTCC CTGGCTCAGA CTTCAGCGTT GGGGCTGACA CAGCACTCAA TACTATCTGC 
ACAACCTGTG ACCGCATCAA GCAGTCAGCA GCTGGCACCA AGCGTCGGGT GTTTATCATT 
GAGACTATGG GTGGCTACTG TGGCTACCTG GCTACCATGG CTGGACTGGC AGCTGGGGCC 
GATGCTGCCT ACATTTTTGA GGAGCCCTTC ACCATTCGAG ACCTGCAGGC AAATGTTGAA 
CATCTGGTGC AAAAGATGAA AACAACTGTG AAAAGGGGCT TGGTGTTAAG GAATGAAAAG 
TGCAATGAGA ACTATACCAC TGACTTCATT TTCAACCTGT ACTCTGAGGA GGGGAAGGGC 
ATCTTCGACA GCAGGAAGAA TGTGCTTGGT CACATGCAGC AGGGTGGGAG CCCAACCCCA 
TTTGATAGGA ATTTTGCCAC TAAGATGGGC GCCAAGGCTA TGAACTGGAT GTCTGGGAAA 
ATCAAAGAGA GTTACCGTAA TGGGCGGATC TTTGCCAATA CTCCAGATTC GGGCTGTGTT 
CTGGGGATGC GTAAGAGGGC TCTGGTCTTC CAACCAGTGG CTGAGCTGAA GGACCAGACA 
GATTTTGAGC ATCGAATCCC CAAGGAACAG TGGTGGCTGA AACTGAGGCC CATCCTCAAA 
ATCCTAGCCA AGTACGAGAT TGACTTGGAC ACTTCAGACC ATGCCCACCT GGAGCACATC 
ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341677.1
CDS	262..2454
Translation	MPKGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354748.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354748.1

ORF Insert Sequence:

ATGCCCAAGG GTTATCAAGG CCTGGTGGAT GGTGGAGATC ACATCAAGGA AGCCACCTGG 
GAGAGCGTTT CGATGATGCT TCAGCTGGGA GGCACGGTGA TTGGAAGTGC CCGGTGCAAG 
GACTTTCGGG AACGAGAAGG ACGACTCCGA GCTGCCTACA ACCTGGTGAA GCGTGGGATC 
ACCAATCTCT GTGTCATTGG GGGTGATGGC AGCCTCACTG GGGCTGACAC CTTCCGTTCT 
GAGTGGAGTG ACTTGTTGAG TGACCTCCAG AAAGCAGGTA AGATCACAGA TGAGGAGGCT 
ACGAAGTCCA GCTACCTGAA CATTGTGGGC CTGGTTGGGT CAATTGACAA TGACTTCTGT 
GGCACCGATA TGACCATTGG CACTGACTCT GCCCTGCATC GGATCATGGA AATTGTAGAT 
GCCATCACTA CCACTGCCCA GAGCCACCAG AGGACATTTG TGTTAGAAGT AATGGGCCGC 
CACTGTGGAT ACCTGGCCCT TGTCACCTCT CTGTCCTGTG GGGCCGACTG GGTTTTTATT 
CCTGAATGTC CACCAGATGA CGACTGGGAG GAACACCTTT GTCGCCGACT CAGCGAGACA 
AGGACCCGTG GTTCTCGTCT CAACATCATC ATTGTGGCTG AGGGTGCAAT TGACAAGAAT 
GGAAAACCAA TCACCTCAGA AGACATCAAG AATCTGGTGG TTAAGCGTCT GGGATATGAC 
ACCCGGGTTA CTGTCTTGGG GCATGTGCAG AGGGGTGGGA CGCCATCAGC CTTTGACAGA 
ATTCTGGGCA GCAGGATGGG TGTGGAAGCA GTGATGGCAC TTTTGGAGGG GACCCCAGAT 
ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT AACCAGGCTG TGCGCCTGCC CCTCATGGAA 
TGTGTCCAGG TGACCAAAGA TGTGACCAAG GCCATGGATG AGAAGAAATT TGACGAAGCC 
CTGAAGCTGA GAGGCCGGAG CTTCATGAAC AACTGGGAGG TGTACAAGCT TCTAGCTCAT 
GTCAGACCCC CGGTATCTAA GAGTGGTTCG CACACAGTGG CTGTGATGAA CGTGGGGGCT 
CCGGCTGCAG GCATGAATGC TGCTGTTCGC TCCACTGTGA GGATTGGCCT TATCCAGGGC 
AACCGAGTGC TCGTTGTCCA TGATGGTTTC GAGGGCCTGG CCAAGGGGCA GATAGAGGAA 
GCTGGCTGGA GCTATGTTGG GGGCTGGACT GGCCAAGGTG GCTCTAAACT TGGGACTAAA 
AGGACTCTAC CCAAGAAGAG CTTTGAACAG ATCAGTGCCA ATATAACTAA GTTTAACATT 
CAGGGCCTTG TCATCATTGG GGGCTTTGAG GCTTACACAG GGGGCCTGGA ACTGATGGAG 
GGCAGGAAGC AGTTTGATGA GCTCTGCATC CCATTTGTGG TCATTCCTGC TACAGTCTCC 
AACAATGTCC CTGGCTCAGA CTTCAGCGTT GGGGCTGACA CAGCACTCAA TACTATCTGC 
ACAACCTGTG ACCGCATCAA GCAGTCAGCA GCTGGCACCA AGCGTCGGGT GTTTATCATT 
GAGACTATGG GTGGCTACTG TGGCTACCTG GCTACCATGG CTGGACTGGC AGCTGGGGCC 
GATGCTGCCT ACATTTTTGA GGAGCCCTTC ACCATTCGAG ACCTGCAGGC AAATGTTGAA 
CATCTGGTGC AAAAGATGAA AACAACTGTG AAAAGGGGCT TGGTGTTAAG GAATGAAAAG 
TGCAATGAGA ACTATACCAC TGACTTCATT TTCAACCTGT ACTCTGAGGA GGGGAAGGGC 
ATCTTCGACA GCAGGAAGAA TGTGCTTGGT CACATGCAGC AGGGTGGGAG CCCAACCCCA 
TTTGATAGGA ATTTTGCCAC TAAGATGGGC GCCAAGGCTA TGAACTGGAT GTCTGGGAAA 
ATCAAAGAGA GTTACCGTAA TGGGCGGATC TTTGCCAATA CTCCAGATTC GGGCTGTGTT 
CTGGGGATGC GTAAGAGGGC TCTGGTCTTC CAACCAGTGG CTGAGCTGAA GGACCAGACA 
GATTTTGAGC ATCGAATCCC CAAGGAACAG TGGTGGCTGA AACTGAGGCC CATCCTCAAA 
ATCCTAGCCA AGTACGAGAT TGACTTGGAC ACTTCAGACC ATGCCCACCT GGAGCACATC 
ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354747.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341676.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 17, mRNA.

Price & Availability↑

CloneID	OHu102861
Clone ID Related Accession (Same CDS sequence)	NM_001354748.1 , NM_001354747.1
Accession Version	NM_001354747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.247584.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2149876 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCAAGG GTTATCAAGG CCTGGTGGAT GGTGGAGATC ACATCAAGGA AGCCACCTGG 
GAGAGCGTTT CGATGATGCT TCAGCTGGGA GGCACGGTGA TTGGAAGTGC CCGGTGCAAG 
GACTTTCGGG AACGAGAAGG ACGACTCCGA GCTGCCTACA ACCTGGTGAA GCGTGGGATC 
ACCAATCTCT GTGTCATTGG GGGTGATGGC AGCCTCACTG GGGCTGACAC CTTCCGTTCT 
GAGTGGAGTG ACTTGTTGAG TGACCTCCAG AAAGCAGGTA AGATCACAGA TGAGGAGGCT 
ACGAAGTCCA GCTACCTGAA CATTGTGGGC CTGGTTGGGT CAATTGACAA TGACTTCTGT 
GGCACCGATA TGACCATTGG CACTGACTCT GCCCTGCATC GGATCATGGA AATTGTAGAT 
GCCATCACTA CCACTGCCCA GAGCCACCAG AGGACATTTG TGTTAGAAGT AATGGGCCGC 
CACTGTGGAT ACCTGGCCCT TGTCACCTCT CTGTCCTGTG GGGCCGACTG GGTTTTTATT 
CCTGAATGTC CACCAGATGA CGACTGGGAG GAACACCTTT GTCGCCGACT CAGCGAGACA 
AGGACCCGTG GTTCTCGTCT CAACATCATC ATTGTGGCTG AGGGTGCAAT TGACAAGAAT 
GGAAAACCAA TCACCTCAGA AGACATCAAG AATCTGGTGG TTAAGCGTCT GGGATATGAC 
ACCCGGGTTA CTGTCTTGGG GCATGTGCAG AGGGGTGGGA CGCCATCAGC CTTTGACAGA 
ATTCTGGGCA GCAGGATGGG TGTGGAAGCA GTGATGGCAC TTTTGGAGGG GACCCCAGAT 
ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT AACCAGGCTG TGCGCCTGCC CCTCATGGAA 
TGTGTCCAGG TGACCAAAGA TGTGACCAAG GCCATGGATG AGAAGAAATT TGACGAAGCC 
CTGAAGCTGA GAGGCCGGAG CTTCATGAAC AACTGGGAGG TGTACAAGCT TCTAGCTCAT 
GTCAGACCCC CGGTATCTAA GAGTGGTTCG CACACAGTGG CTGTGATGAA CGTGGGGGCT 
CCGGCTGCAG GCATGAATGC TGCTGTTCGC TCCACTGTGA GGATTGGCCT TATCCAGGGC 
AACCGAGTGC TCGTTGTCCA TGATGGTTTC GAGGGCCTGG CCAAGGGGCA GATAGAGGAA 
GCTGGCTGGA GCTATGTTGG GGGCTGGACT GGCCAAGGTG GCTCTAAACT TGGGACTAAA 
AGGACTCTAC CCAAGAAGAG CTTTGAACAG ATCAGTGCCA ATATAACTAA GTTTAACATT 
CAGGGCCTTG TCATCATTGG GGGCTTTGAG GCTTACACAG GGGGCCTGGA ACTGATGGAG 
GGCAGGAAGC AGTTTGATGA GCTCTGCATC CCATTTGTGG TCATTCCTGC TACAGTCTCC 
AACAATGTCC CTGGCTCAGA CTTCAGCGTT GGGGCTGACA CAGCACTCAA TACTATCTGC 
ACAACCTGTG ACCGCATCAA GCAGTCAGCA GCTGGCACCA AGCGTCGGGT GTTTATCATT 
GAGACTATGG GTGGCTACTG TGGCTACCTG GCTACCATGG CTGGACTGGC AGCTGGGGCC 
GATGCTGCCT ACATTTTTGA GGAGCCCTTC ACCATTCGAG ACCTGCAGGC AAATGTTGAA 
CATCTGGTGC AAAAGATGAA AACAACTGTG AAAAGGGGCT TGGTGTTAAG GAATGAAAAG 
TGCAATGAGA ACTATACCAC TGACTTCATT TTCAACCTGT ACTCTGAGGA GGGGAAGGGC 
ATCTTCGACA GCAGGAAGAA TGTGCTTGGT CACATGCAGC AGGGTGGGAG CCCAACCCCA 
TTTGATAGGA ATTTTGCCAC TAAGATGGGC GCCAAGGCTA TGAACTGGAT GTCTGGGAAA 
ATCAAAGAGA GTTACCGTAA TGGGCGGATC TTTGCCAATA CTCCAGATTC GGGCTGTGTT 
CTGGGGATGC GTAAGAGGGC TCTGGTCTTC CAACCAGTGG CTGAGCTGAA GGACCAGACA 
GATTTTGAGC ATCGAATCCC CAAGGAACAG TGGTGGCTGA AACTGAGGCC CATCCTCAAA 
ATCCTAGCCA AGTACGAGAT TGACTTGGAC ACTTCAGACC ATGCCCACCT GGAGCACATC 
ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341676.1
CDS	252..2444
Translation	MPKGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354747.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354747.1

ORF Insert Sequence:

ATGCCCAAGG GTTATCAAGG CCTGGTGGAT GGTGGAGATC ACATCAAGGA AGCCACCTGG 
GAGAGCGTTT CGATGATGCT TCAGCTGGGA GGCACGGTGA TTGGAAGTGC CCGGTGCAAG 
GACTTTCGGG AACGAGAAGG ACGACTCCGA GCTGCCTACA ACCTGGTGAA GCGTGGGATC 
ACCAATCTCT GTGTCATTGG GGGTGATGGC AGCCTCACTG GGGCTGACAC CTTCCGTTCT 
GAGTGGAGTG ACTTGTTGAG TGACCTCCAG AAAGCAGGTA AGATCACAGA TGAGGAGGCT 
ACGAAGTCCA GCTACCTGAA CATTGTGGGC CTGGTTGGGT CAATTGACAA TGACTTCTGT 
GGCACCGATA TGACCATTGG CACTGACTCT GCCCTGCATC GGATCATGGA AATTGTAGAT 
GCCATCACTA CCACTGCCCA GAGCCACCAG AGGACATTTG TGTTAGAAGT AATGGGCCGC 
CACTGTGGAT ACCTGGCCCT TGTCACCTCT CTGTCCTGTG GGGCCGACTG GGTTTTTATT 
CCTGAATGTC CACCAGATGA CGACTGGGAG GAACACCTTT GTCGCCGACT CAGCGAGACA 
AGGACCCGTG GTTCTCGTCT CAACATCATC ATTGTGGCTG AGGGTGCAAT TGACAAGAAT 
GGAAAACCAA TCACCTCAGA AGACATCAAG AATCTGGTGG TTAAGCGTCT GGGATATGAC 
ACCCGGGTTA CTGTCTTGGG GCATGTGCAG AGGGGTGGGA CGCCATCAGC CTTTGACAGA 
ATTCTGGGCA GCAGGATGGG TGTGGAAGCA GTGATGGCAC TTTTGGAGGG GACCCCAGAT 
ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT AACCAGGCTG TGCGCCTGCC CCTCATGGAA 
TGTGTCCAGG TGACCAAAGA TGTGACCAAG GCCATGGATG AGAAGAAATT TGACGAAGCC 
CTGAAGCTGA GAGGCCGGAG CTTCATGAAC AACTGGGAGG TGTACAAGCT TCTAGCTCAT 
GTCAGACCCC CGGTATCTAA GAGTGGTTCG CACACAGTGG CTGTGATGAA CGTGGGGGCT 
CCGGCTGCAG GCATGAATGC TGCTGTTCGC TCCACTGTGA GGATTGGCCT TATCCAGGGC 
AACCGAGTGC TCGTTGTCCA TGATGGTTTC GAGGGCCTGG CCAAGGGGCA GATAGAGGAA 
GCTGGCTGGA GCTATGTTGG GGGCTGGACT GGCCAAGGTG GCTCTAAACT TGGGACTAAA 
AGGACTCTAC CCAAGAAGAG CTTTGAACAG ATCAGTGCCA ATATAACTAA GTTTAACATT 
CAGGGCCTTG TCATCATTGG GGGCTTTGAG GCTTACACAG GGGGCCTGGA ACTGATGGAG 
GGCAGGAAGC AGTTTGATGA GCTCTGCATC CCATTTGTGG TCATTCCTGC TACAGTCTCC 
AACAATGTCC CTGGCTCAGA CTTCAGCGTT GGGGCTGACA CAGCACTCAA TACTATCTGC 
ACAACCTGTG ACCGCATCAA GCAGTCAGCA GCTGGCACCA AGCGTCGGGT GTTTATCATT 
GAGACTATGG GTGGCTACTG TGGCTACCTG GCTACCATGG CTGGACTGGC AGCTGGGGCC 
GATGCTGCCT ACATTTTTGA GGAGCCCTTC ACCATTCGAG ACCTGCAGGC AAATGTTGAA 
CATCTGGTGC AAAAGATGAA AACAACTGTG AAAAGGGGCT TGGTGTTAAG GAATGAAAAG 
TGCAATGAGA ACTATACCAC TGACTTCATT TTCAACCTGT ACTCTGAGGA GGGGAAGGGC 
ATCTTCGACA GCAGGAAGAA TGTGCTTGGT CACATGCAGC AGGGTGGGAG CCCAACCCCA 
TTTGATAGGA ATTTTGCCAC TAAGATGGGC GCCAAGGCTA TGAACTGGAT GTCTGGGAAA 
ATCAAAGAGA GTTACCGTAA TGGGCGGATC TTTGCCAATA CTCCAGATTC GGGCTGTGTT 
CTGGGGATGC GTAAGAGGGC TCTGGTCTTC CAACCAGTGG CTGAGCTGAA GGACCAGACA 
GATTTTGAGC ATCGAATCCC CAAGGAACAG TGGTGGCTGA AACTGAGGCC CATCCTCAAA 
ATCCTAGCCA AGTACGAGAT TGACTTGGAC ACTTCAGACC ATGCCCACCT GGAGCACATC 
ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354746.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341675.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 16, mRNA.

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CloneID	OHu102886
Clone ID Related Accession (Same CDS sequence)	NM_001354746.1
Accession Version	NM_001354746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.5524.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGACCA AAGATGTGAC CAAGGCCATG 
GATGAGAAGA AATTTGACGA AGCCCTGAAG CTGAGAGGCC GGAGCTTCAT GAACAACTGG 
GAGGTGTACA AGCTTCTAGC TCATGTCAGA CCCCCGGTAT CTAAGAGTGG TTCGCACACA 
GTGGCTGTGA TGAACGTGGG GGCTCCGGCT GCAGGCATGA ATGCTGCTGT TCGCTCCACT 
GTGAGGATTG GCCTTATCCA GGGCAACCGA GTGCTCGTTG TCCATGATGG TTTCGAGGGC 
CTGGCCAAGG GGCAGATAGA GGAAGCTGGC TGGAGCTATG TTGGGGGCTG GACTGGCCAA 
GGTGGCTCTA AACTTGGGAC TAAAAGGACT CTACCCAAGA AGAGCTTTGA ACAGATCAGT 
GCCAATATAA CTAAGTTTAA CATTCAGGGC CTTGTCATCA TTGGGGGCTT TGAGGCTTAC 
ACAGGGGGCC TGGAACTGAT GGAGGGCAGG AAGCAGTTTG ATGAGCTCTG CATCCCATTT 
GTGGTCATTC CTGCTACAGT CTCCAACAAT GTCCCTGGCT CAGACTTCAG CGTTGGGGCT 
GACACAGCAC TCAATACTAT CTGCACAACC TGTGACCGCA TCAAGCAGTC AGCAGCTGGC 
ACCAAGCGTC GGGTGTTTAT CATTGAGACT ATGGGTGGCT ACTGTGGCTA CCTGGCTACC 
ATGGCTGGAC TGGCAGCTGG GGCCGATGCT GCCTACATTT TTGAGGAGCC CTTCACCATT 
CGAGACCTGC AGGCAAATGT TGAACATCTG GTGCAAAAGA TGAAAACAAC TGTGAAAAGG 
GGCTTGGTGT TAAGGAATGA AAAGTGCAAT GAGAACTATA CCACTGACTT CATTTTCAAC 
CTGTACTCTG AGGAGGGGAA GGGCATCTTC GACAGCAGGA AGAATGTGCT TGGTCACATG 
CAGCAGGGTG GGAGCCCAAC CCCATTTGAT AGGAATTTTG CCACTAAGAT GGGCGCCAAG 
GCTATGAACT GGATGTCTGG GAAAATCAAA GAGAGTTACC GTAATGGGCG GATCTTTGCC 
AATACTCCAG ATTCGGGCTG TGTTCTGGGG ATGCGTAAGA GGGCTCTGGT CTTCCAACCA 
GTGGCTGAGC TGAAGGACCA GACAGATTTT GAGCATCGAA TCCCCAAGGA ACAGTGGTGG 
CTGAAACTGA GGCCCATCCT CAAAATCCTA GCCAAGTACG AGATTGACTT GGACACTTCA 
GACCATGCCC ACCTGGAGCA CATCACCCGG AAGCGGTCCG GGGAAGCTGC CGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341675.1
CDS	186..2402
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354746.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354746.1

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATCTGGTGG TTAAGCGTCT GGGATATGAC ACCCGGGTTA CTGTCTTGGG GCATGTGCAG 
AGGGGTGGGA CGCCATCAGC CTTTGACAGA ATTCTGACCA AAGATGTGAC CAAGGCCATG 
GATGAGAAGA AATTTGACGA AGCCCTGAAG CTGAGAGGCC GGAGCTTCAT GAACAACTGG 
GAGGTGTACA AGCTTCTAGC TCATGTCAGA CCCCCGGTAT CTAAGAGTGG TTCGCACACA 
GTGGCTGTGA TGAACGTGGG GGCTCCGGCT GCAGGCATGA ATGCTGCTGT TCGCTCCACT 
GTGAGGATTG GCCTTATCCA GGGCAACCGA GTGCTCGTTG TCCATGATGG TTTCGAGGGC 
CTGGCCAAGG GGCAGATAGA GGAAGCTGGC TGGAGCTATG TTGGGGGCTG GACTGGCCAA 
GGTGGCTCTA AACTTGGGAC TAAAAGGACT CTACCCAAGA AGAGCTTTGA ACAGATCAGT 
GCCAATATAA CTAAGTTTAA CATTCAGGGC CTTGTCATCA TTGGGGGCTT TGAGGCTTAC 
ACAGGGGGCC TGGAACTGAT GGAGGGCAGG AAGCAGTTTG ATGAGCTCTG CATCCCATTT 
GTGGTCATTC CTGCTACAGT CTCCAACAAT GTCCCTGGCT CAGACTTCAG CGTTGGGGCT 
GACACAGCAC TCAATACTAT CTGCACAACC TGTGACCGCA TCAAGCAGTC AGCAGCTGGC 
ACCAAGCGTC GGGTGTTTAT CATTGAGACT ATGGGTGGCT ACTGTGGCTA CCTGGCTACC 
ATGGCTGGAC TGGCAGCTGG GGCCGATGCT GCCTACATTT TTGAGGAGCC CTTCACCATT 
CGAGACCTGC AGGCAAATGT TGAACATCTG GTGCAAAAGA TGAAAACAAC TGTGAAAAGG 
GGCTTGGTGT TAAGGAATGA AAAGTGCAAT GAGAACTATA CCACTGACTT CATTTTCAAC 
CTGTACTCTG AGGAGGGGAA GGGCATCTTC GACAGCAGGA AGAATGTGCT TGGTCACATG 
CAGCAGGGTG GGAGCCCAAC CCCATTTGAT AGGAATTTTG CCACTAAGAT GGGCGCCAAG 
GCTATGAACT GGATGTCTGG GAAAATCAAA GAGAGTTACC GTAATGGGCG GATCTTTGCC 
AATACTCCAG ATTCGGGCTG TGTTCTGGGG ATGCGTAAGA GGGCTCTGGT CTTCCAACCA 
GTGGCTGAGC TGAAGGACCA GACAGATTTT GAGCATCGAA TCCCCAAGGA ACAGTGGTGG 
CTGAAACTGA GGCCCATCCT CAAAATCCTA GCCAAGTACG AGATTGACTT GGACACTTCA 
GACCATGCCC ACCTGGAGCA CATCACCCGG AAGCGGTCCG GGGAAGCTGC CGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354741.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341670.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OHu102915
Clone ID Related Accession (Same CDS sequence)	NM_001354741.1
Accession Version	NM_001354741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.147898.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153031, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGCAAA GGGAAGAGTG GATCATGACC CATGAAGAGC ACCATGCAGC CAAAACCCTG 
GGGATTGGCA AAGCCATTGC TGTCTTAACC TCTGGTGGAG ATGCCCAAGG TATGAATGCT 
GCTGTCAGGG CTGTGGTTCG AGTTGGTATC TTCACCGGTG CCCGTGTCTT CTTTGTCCAT 
GAGGGTTATC AAGGCCTGGT GGATGGTGGA GATCACATCA AGGAAGCCAC CTGGGAGAGC 
GTTTCGATGA TGCTTCAGCT GGGAGGCACG GTGATTGGAA GTGCCCGGTG CAAGGACTTT 
CGGGAACGAG AAGGACGACT CCGAGCTGCC TACAACCTGG TGAAGCGTGG GATCACCAAT 
CTCTGTGTCA TTGGGGGTGA TGGCAGCCTC ACTGGGGCTG ACACCTTCCG TTCTGAGTGG 
AGTGACTTGT TGAGTGACCT CCAGAAAGCA GGTAAGATCA CAGATGAGGA GGCTACGAAG 
TCCAGCTACC TGAACATTGT GGGCCTGGTT GGGTCAATTG ACAATGACTT CTGTGGCACC 
GATATGACCA TTGGCACTGA CTCTGCCCTG CATCGGATCA TGGAAATTGT AGATGCCATC 
ACTACCACTG CCCAGAGCCA CCAGAGGACA TTTGTGTTAG AAGTAATGGG CCGCCACTGT 
GGATACCTGG CCCTTGTCAC CTCTCTGTCC TGTGGGGCCG ACTGGGTTTT TATTCCTGAA 
TGTCCACCAG ATGACGACTG GGAGGAACAC CTTTGTCGCC GACTCAGCGA GACAAGGACC 
CGTGGTTCTC GTCTCAACAT CATCATTGTG GCTGAGGGTG CAATTGACAA GAATGGAAAA 
CCAATCACCT CAGAAGACAT CAAGAATCTG GTGGTTAAGC GTCTGGGATA TGACACCCGG 
GTTACTGTCT TGGGGCATGT GCAGAGGGGT GGGACGCCAT CAGCCTTTGA CAGAATTCTG 
GGCAGCAGGA TGGGTGTGGA AGCAGTGATG GCACTTTTGG AGGGGACCCC AGATACCCCA 
GCCTGTGTAG TGAGCCTCTC TGGTAACCAG GCTGTGCGCC TGCCCCTCAT GGAATGTGTC 
CAGGTGACCA AAGATGTGAC CAAGGCCATG GATGAGAAGA AATTTGACGA AGCCCTGAAG 
CTGAGAGGCC GGAGCTTCAT GAACAACTGG GAGGTGTACA AGCTTCTAGC TCATGTCAGA 
CCCCCGGTAT CTAAGAGTGG TTCGCACACA GTGGCTGTGA TGAACGTGGG GGCTCCGGCT 
GCAGGCATGA ATGCTGCTGT TCGCTCCACT GTGAGGATTG GCCTTATCCA GGGCAACCGA 
GTGCTCGTTG TCCATGATGG TTTCGAGGGC CTGGCCAAGG GGCAGATAGA GGAAGCTGGC 
TGGAGCTATG TTGGGGGCTG GACTGGCCAA GGTGGCTCTA AACTTGGGAC TAAAAGGACT 
CTACCCAAGA AGAGCTTTGA ACAGATCAGT GCCAATATAA CTAAGTTTAA CATTCAGGGC 
CTTGTCATCA TTGGGGGCTT TGAGGCTTAC ACAGGGGGCC TGGAACTGAT GGAGGGCAGG 
AAGCAGTTTG ATGAGCTCTG CATCCCATTT GTGGTCATTC CTGCTACAGT CTCCAACAAT 
GTCCCTGGCT CAGACTTCAG CGTTGGGGCT GACACAGCAC TCAATACTAT CTGCACAACC 
TGTGACCGCA TCAAGCAGTC AGCAGCTGGC ACCAAGCGTC GGGTGTTTAT CATTGAGACT 
ATGGGTGGCT ACTGTGGCTA CCTGGCTACC ATGGCTGGAC TGGCAGCTGG GGCCGATGCT 
GCCTACATTT TTGAGGAGCC CTTCACCATT CGAGACCTGC AGGCAAATGT TGAACATCTG 
GTGCAAAAGA TGAAAACAAC TGTGAAAAGG GGCTTGGTGT TAAGGAATGA AAAGTGCAAT 
GAGAACTATA CCACTGACTT CATTTTCAAC CTGTACTCTG AGGAGGGGAA GGGCATCTTC 
GACAGCAGGA AGAATGTGCT TGGTCACATG CAGCAGGGTG GGAGCCCAAC CCCATTTGAT 
AGGAATTTTG CCACTAAGAT GGGCGCCAAG GCTATGAACT GGATGTCTGG GAAAATCAAA 
GAGAGTTACC GTAATGGGCG GATCTTTGCC AATACTCCAG ATTCGGGCTG TGTTCTGGGG 
ATGCGTAAGA GGGCTCTGGT CTTCCAACCA GTGGCTGAGC TGAAGGACCA GACAGATTTT 
GAGCATCGAA TCCCCAAGGA ACAGTGGTGG CTGAAACTGA GGCCCATCCT CAAAATCCTA 
GCCAAGTACG AGATTGACTT GGACACTTCA GACCATGCCC ACCTGGAGCA CATCACCCGG 
AAGCGGTCCG GGGAAGCTGC CGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341670.1
CDS	248..2614
Translation	MKQREEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354741.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354741.1

ORF Insert Sequence:

ATGAAGCAAA GGGAAGAGTG GATCATGACC CATGAAGAGC ACCATGCAGC CAAAACCCTG 
GGGATTGGCA AAGCCATTGC TGTCTTAACC TCTGGTGGAG ATGCCCAAGG TATGAATGCT 
GCTGTCAGGG CTGTGGTTCG AGTTGGTATC TTCACCGGTG CCCGTGTCTT CTTTGTCCAT 
GAGGGTTATC AAGGCCTGGT GGATGGTGGA GATCACATCA AGGAAGCCAC CTGGGAGAGC 
GTTTCGATGA TGCTTCAGCT GGGAGGCACG GTGATTGGAA GTGCCCGGTG CAAGGACTTT 
CGGGAACGAG AAGGACGACT CCGAGCTGCC TACAACCTGG TGAAGCGTGG GATCACCAAT 
CTCTGTGTCA TTGGGGGTGA TGGCAGCCTC ACTGGGGCTG ACACCTTCCG TTCTGAGTGG 
AGTGACTTGT TGAGTGACCT CCAGAAAGCA GGTAAGATCA CAGATGAGGA GGCTACGAAG 
TCCAGCTACC TGAACATTGT GGGCCTGGTT GGGTCAATTG ACAATGACTT CTGTGGCACC 
GATATGACCA TTGGCACTGA CTCTGCCCTG CATCGGATCA TGGAAATTGT AGATGCCATC 
ACTACCACTG CCCAGAGCCA CCAGAGGACA TTTGTGTTAG AAGTAATGGG CCGCCACTGT 
GGATACCTGG CCCTTGTCAC CTCTCTGTCC TGTGGGGCCG ACTGGGTTTT TATTCCTGAA 
TGTCCACCAG ATGACGACTG GGAGGAACAC CTTTGTCGCC GACTCAGCGA GACAAGGACC 
CGTGGTTCTC GTCTCAACAT CATCATTGTG GCTGAGGGTG CAATTGACAA GAATGGAAAA 
CCAATCACCT CAGAAGACAT CAAGAATCTG GTGGTTAAGC GTCTGGGATA TGACACCCGG 
GTTACTGTCT TGGGGCATGT GCAGAGGGGT GGGACGCCAT CAGCCTTTGA CAGAATTCTG 
GGCAGCAGGA TGGGTGTGGA AGCAGTGATG GCACTTTTGG AGGGGACCCC AGATACCCCA 
GCCTGTGTAG TGAGCCTCTC TGGTAACCAG GCTGTGCGCC TGCCCCTCAT GGAATGTGTC 
CAGGTGACCA AAGATGTGAC CAAGGCCATG GATGAGAAGA AATTTGACGA AGCCCTGAAG 
CTGAGAGGCC GGAGCTTCAT GAACAACTGG GAGGTGTACA AGCTTCTAGC TCATGTCAGA 
CCCCCGGTAT CTAAGAGTGG TTCGCACACA GTGGCTGTGA TGAACGTGGG GGCTCCGGCT 
GCAGGCATGA ATGCTGCTGT TCGCTCCACT GTGAGGATTG GCCTTATCCA GGGCAACCGA 
GTGCTCGTTG TCCATGATGG TTTCGAGGGC CTGGCCAAGG GGCAGATAGA GGAAGCTGGC 
TGGAGCTATG TTGGGGGCTG GACTGGCCAA GGTGGCTCTA AACTTGGGAC TAAAAGGACT 
CTACCCAAGA AGAGCTTTGA ACAGATCAGT GCCAATATAA CTAAGTTTAA CATTCAGGGC 
CTTGTCATCA TTGGGGGCTT TGAGGCTTAC ACAGGGGGCC TGGAACTGAT GGAGGGCAGG 
AAGCAGTTTG ATGAGCTCTG CATCCCATTT GTGGTCATTC CTGCTACAGT CTCCAACAAT 
GTCCCTGGCT CAGACTTCAG CGTTGGGGCT GACACAGCAC TCAATACTAT CTGCACAACC 
TGTGACCGCA TCAAGCAGTC AGCAGCTGGC ACCAAGCGTC GGGTGTTTAT CATTGAGACT 
ATGGGTGGCT ACTGTGGCTA CCTGGCTACC ATGGCTGGAC TGGCAGCTGG GGCCGATGCT 
GCCTACATTT TTGAGGAGCC CTTCACCATT CGAGACCTGC AGGCAAATGT TGAACATCTG 
GTGCAAAAGA TGAAAACAAC TGTGAAAAGG GGCTTGGTGT TAAGGAATGA AAAGTGCAAT 
GAGAACTATA CCACTGACTT CATTTTCAAC CTGTACTCTG AGGAGGGGAA GGGCATCTTC 
GACAGCAGGA AGAATGTGCT TGGTCACATG CAGCAGGGTG GGAGCCCAAC CCCATTTGAT 
AGGAATTTTG CCACTAAGAT GGGCGCCAAG GCTATGAACT GGATGTCTGG GAAAATCAAA 
GAGAGTTACC GTAATGGGCG GATCTTTGCC AATACTCCAG ATTCGGGCTG TGTTCTGGGG 
ATGCGTAAGA GGGCTCTGGT CTTCCAACCA GTGGCTGAGC TGAAGGACCA GACAGATTTT 
GAGCATCGAA TCCCCAAGGA ACAGTGGTGG CTGAAACTGA GGCCCATCCT CAAAATCCTA 
GCCAAGTACG AGATTGACTT GGACACTTCA GACCATGCCC ACCTGGAGCA CATCACCCGG 
AAGCGGTCCG GGGAAGCTGC CGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001354740.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341669.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu102933
Clone ID Related Accession (Same CDS sequence)	NM_001354740.1
Accession Version	NM_001354740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2 and AC074029.15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.260240.1, SRR1660807.139217.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCGTCC ATCACCCCTC CCCCCTTTCC CAAGGACAAT CTGCAAGAAA GCAGCGGCGG 
AGGAGAGCTA AGACTAAAAG GCAAGAGGGG CCATTGAGTG AAGAGCAAGG GGGAGAGCTG 
GGAGTGAGGT GGGAAGAGTG GATCATGACC CATGAAGAGC ACCATGCAGC CAAAACCCTG 
GGGATTGGCA AAGCCATTGC TGTCTTAACC TCTGGTGGAG ATGCCCAAGG TATGAATGCT 
GCTGTCAGGG CTGTGGTTCG AGTTGGTATC TTCACCGGTG CCCGTGTCTT CTTTGTCCAT 
GAGGGTTATC AAGGCCTGGT GGATGGTGGA GATCACATCA AGGAAGCCAC CTGGGAGAGC 
GTTTCGATGA TGCTTCAGCT GGGAGGCACG GTGATTGGAA GTGCCCGGTG CAAGGACTTT 
CGGGAACGAG AAGGACGACT CCGAGCTGCC TACAACCTGG TGAAGCGTGG GATCACCAAT 
CTCTGTGTCA TTGGGGGTGA TGGCAGCCTC ACTGGGGCTG ACACCTTCCG TTCTGAGTGG 
AGTGACTTGT TGAGTGACCT CCAGAAAGCA GGTAAGATCA CAGATGAGGA GGCTACGAAG 
TCCAGCTACC TGAACATTGT GGGCCTGGTT GGGTCAATTG ACAATGACTT CTGTGGCACC 
GATATGACCA TTGGCACTGA CTCTGCCCTG CATCGGATCA TGGAAATTGT AGATGCCATC 
ACTACCACTG CCCAGAGCCA CCAGAGGACA TTTGTGTTAG AAGTAATGGG CCGCCACTGT 
GGATACCTGG CCCTTGTCAC CTCTCTGTCC TGTGGGGCCG ACTGGGTTTT TATTCCTGAA 
TGTCCACCAG ATGACGACTG GGAGGAACAC CTTTGTCGCC GACTCAGCGA GACAAGGACC 
CGTGGTTCTC GTCTCAACAT CATCATTGTG GCTGAGGGTG CAATTGACAA GAATGGAAAA 
CCAATCACCT CAGAAGACAT CAAGAATCTG GTGGTTAAGC GTCTGGGATA TGACACCCGG 
GTTACTGTCT TGGGGCATGT GCAGAGGGGT GGGACGCCAT CAGCCTTTGA CAGAATTCTG 
GGCAGCAGGA TGGGTGTGGA AGCAGTGATG GCACTTTTGG AGGGGACCCC AGATACCCCA 
GCCTGTGTAG TGAGCCTCTC TGGTAACCAG GCTGTGCGCC TGCCCCTCAT GGAATGTGTC 
CAGGTGACCA AAGATGTGAC CAAGGCCATG GATGAGAAGA AATTTGACGA AGCCCTGAAG 
CTGAGAGGCC GGAGCTTCAT GAACAACTGG GAGGTGTACA AGCTTCTAGC TCATGTCAGA 
CCCCCGGTAT CTAAGAGTGG TTCGCACACA GTGGCTGTGA TGAACGTGGG GGCTCCGGCT 
GCAGGCATGA ATGCTGCTGT TCGCTCCACT GTGAGGATTG GCCTTATCCA GGGCAACCGA 
GTGCTCGTTG TCCATGATGG TTTCGAGGGC CTGGCCAAGG GGCAGATAGA GGAAGCTGGC 
TGGAGCTATG TTGGGGGCTG GACTGGCCAA GGTGGCTCTA AACTTGGGAC TAAAAGGACT 
CTACCCAAGA AGAGCTTTGA ACAGATCAGT GCCAATATAA CTAAGTTTAA CATTCAGGGC 
CTTGTCATCA TTGGGGGCTT TGAGGCTTAC ACAGGGGGCC TGGAACTGAT GGAGGGCAGG 
AAGCAGTTTG ATGAGCTCTG CATCCCATTT GTGGTCATTC CTGCTACAGT CTCCAACAAT 
GTCCCTGGCT CAGACTTCAG CGTTGGGGCT GACACAGCAC TCAATACTAT CTGCACAACC 
TGTGACCGCA TCAAGCAGTC AGCAGCTGGC ACCAAGCGTC GGGTGTTTAT CATTGAGACT 
ATGGGTGGCT ACTGTGGCTA CCTGGCTACC ATGGCTGGAC TGGCAGCTGG GGCCGATGCT 
GCCTACATTT TTGAGGAGCC CTTCACCATT CGAGACCTGC AGGCAAATGT TGAACATCTG 
GTGCAAAAGA TGAAAACAAC TGTGAAAAGG GGCTTGGTGT TAAGGAATGA AAAGTGCAAT 
GAGAACTATA CCACTGACTT CATTTTCAAC CTGTACTCTG AGGAGGGGAA GGGCATCTTC 
GACAGCAGGA AGAATGTGCT TGGTCACATG CAGCAGGGTG GGAGCCCAAC CCCATTTGAT 
AGGAATTTTG CCACTAAGAT GGGCGCCAAG GCTATGAACT GGATGTCTGG GAAAATCAAA 
GAGAGTTACC GTAATGGGCG GATCTTTGCC AATACTCCAG ATTCGGGCTG TGTTCTGGGG 
ATGCGTAAGA GGGCTCTGGT CTTCCAACCA GTGGCTGAGC TGAAGGACCA GACAGATTTT 
GAGCATCGAA TCCCCAAGGA ACAGTGGTGG CTGAAACTGA GGCCCATCCT CAAAATCCTA 
GCCAAGTACG AGATTGACTT GGACACTTCA GACCATGCCC ACCTGGAGCA CATCACCCGG 
AAGCGGTCCG GGGAAGCTGC CGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341669.1
CDS	98..2584
Translation	MFVHHPSPLSQGQSARKQRRRRAKTKRQEGPLSEEQGGELGVRWEEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001354740.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354740.1

ORF Insert Sequence:

ATGTTCGTCC ATCACCCCTC CCCCCTTTCC CAAGGACAAT CTGCAAGAAA GCAGCGGCGG 
AGGAGAGCTA AGACTAAAAG GCAAGAGGGG CCATTGAGTG AAGAGCAAGG GGGAGAGCTG 
GGAGTGAGGT GGGAAGAGTG GATCATGACC CATGAAGAGC ACCATGCAGC CAAAACCCTG 
GGGATTGGCA AAGCCATTGC TGTCTTAACC TCTGGTGGAG ATGCCCAAGG TATGAATGCT 
GCTGTCAGGG CTGTGGTTCG AGTTGGTATC TTCACCGGTG CCCGTGTCTT CTTTGTCCAT 
GAGGGTTATC AAGGCCTGGT GGATGGTGGA GATCACATCA AGGAAGCCAC CTGGGAGAGC 
GTTTCGATGA TGCTTCAGCT GGGAGGCACG GTGATTGGAA GTGCCCGGTG CAAGGACTTT 
CGGGAACGAG AAGGACGACT CCGAGCTGCC TACAACCTGG TGAAGCGTGG GATCACCAAT 
CTCTGTGTCA TTGGGGGTGA TGGCAGCCTC ACTGGGGCTG ACACCTTCCG TTCTGAGTGG 
AGTGACTTGT TGAGTGACCT CCAGAAAGCA GGTAAGATCA CAGATGAGGA GGCTACGAAG 
TCCAGCTACC TGAACATTGT GGGCCTGGTT GGGTCAATTG ACAATGACTT CTGTGGCACC 
GATATGACCA TTGGCACTGA CTCTGCCCTG CATCGGATCA TGGAAATTGT AGATGCCATC 
ACTACCACTG CCCAGAGCCA CCAGAGGACA TTTGTGTTAG AAGTAATGGG CCGCCACTGT 
GGATACCTGG CCCTTGTCAC CTCTCTGTCC TGTGGGGCCG ACTGGGTTTT TATTCCTGAA 
TGTCCACCAG ATGACGACTG GGAGGAACAC CTTTGTCGCC GACTCAGCGA GACAAGGACC 
CGTGGTTCTC GTCTCAACAT CATCATTGTG GCTGAGGGTG CAATTGACAA GAATGGAAAA 
CCAATCACCT CAGAAGACAT CAAGAATCTG GTGGTTAAGC GTCTGGGATA TGACACCCGG 
GTTACTGTCT TGGGGCATGT GCAGAGGGGT GGGACGCCAT CAGCCTTTGA CAGAATTCTG 
GGCAGCAGGA TGGGTGTGGA AGCAGTGATG GCACTTTTGG AGGGGACCCC AGATACCCCA 
GCCTGTGTAG TGAGCCTCTC TGGTAACCAG GCTGTGCGCC TGCCCCTCAT GGAATGTGTC 
CAGGTGACCA AAGATGTGAC CAAGGCCATG GATGAGAAGA AATTTGACGA AGCCCTGAAG 
CTGAGAGGCC GGAGCTTCAT GAACAACTGG GAGGTGTACA AGCTTCTAGC TCATGTCAGA 
CCCCCGGTAT CTAAGAGTGG TTCGCACACA GTGGCTGTGA TGAACGTGGG GGCTCCGGCT 
GCAGGCATGA ATGCTGCTGT TCGCTCCACT GTGAGGATTG GCCTTATCCA GGGCAACCGA 
GTGCTCGTTG TCCATGATGG TTTCGAGGGC CTGGCCAAGG GGCAGATAGA GGAAGCTGGC 
TGGAGCTATG TTGGGGGCTG GACTGGCCAA GGTGGCTCTA AACTTGGGAC TAAAAGGACT 
CTACCCAAGA AGAGCTTTGA ACAGATCAGT GCCAATATAA CTAAGTTTAA CATTCAGGGC 
CTTGTCATCA TTGGGGGCTT TGAGGCTTAC ACAGGGGGCC TGGAACTGAT GGAGGGCAGG 
AAGCAGTTTG ATGAGCTCTG CATCCCATTT GTGGTCATTC CTGCTACAGT CTCCAACAAT 
GTCCCTGGCT CAGACTTCAG CGTTGGGGCT GACACAGCAC TCAATACTAT CTGCACAACC 
TGTGACCGCA TCAAGCAGTC AGCAGCTGGC ACCAAGCGTC GGGTGTTTAT CATTGAGACT 
ATGGGTGGCT ACTGTGGCTA CCTGGCTACC ATGGCTGGAC TGGCAGCTGG GGCCGATGCT 
GCCTACATTT TTGAGGAGCC CTTCACCATT CGAGACCTGC AGGCAAATGT TGAACATCTG 
GTGCAAAAGA TGAAAACAAC TGTGAAAAGG GGCTTGGTGT TAAGGAATGA AAAGTGCAAT 
GAGAACTATA CCACTGACTT CATTTTCAAC CTGTACTCTG AGGAGGGGAA GGGCATCTTC 
GACAGCAGGA AGAATGTGCT TGGTCACATG CAGCAGGGTG GGAGCCCAAC CCCATTTGAT 
AGGAATTTTG CCACTAAGAT GGGCGCCAAG GCTATGAACT GGATGTCTGG GAAAATCAAA 
GAGAGTTACC GTAATGGGCG GATCTTTGCC AATACTCCAG ATTCGGGCTG TGTTCTGGGG 
ATGCGTAAGA GGGCTCTGGT CTTCCAACCA GTGGCTGAGC TGAAGGACCA GACAGATTTT 
GAGCATCGAA TCCCCAAGGA ACAGTGGTGG CTGAAACTGA GGCCCATCCT CAAAATCCTA 
GCCAAGTACG AGATTGACTT GGACACTTCA GACCATGCCC ACCTGGAGCA CATCACCCGG 
AAGCGGTCCG GGGAAGCTGC CGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( NM_001363619.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350548.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 25, mRNA.

Price & Availability↑

CloneID	OHu106940
Clone ID Related Accession (Same CDS sequence)	NM_001363619.1
Accession Version	NM_001363619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004801.2, KU178252.1 and AC074029.15. On May 24, 2018 this sequence version replaced XM_024449023.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.233142.1, SRR1803615.121847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATGGCAGCA GGATGGGTGT GGAAGCAGTG ATGGCACTTT TGGAGGGGAC CCCAGATACC 
CCAGCCTGTG TAGTGAGCCT CTCTGGTAAC CAGGCTGTGC GCCTGCCCCT CATGGAATGT 
GTCCAGGTGA CCAAAGATGT GACCAAGGCC ATGGATGAGA AGAAATTTGA CGAAGCCCTG 
AAGCTGAGAG GCCGGAGCTT CATGAACAAC TGGGAGGTGT ACAAGCTTCT AGCTCATGTC 
AGACCCCCGG TATCTAAGAG TGGTTCGCAC ACAGTGGCTG TGATGAACGT GGGGGCTCCG 
GCTGCAGGCA TGAATGCTGC TGTTCGCTCC ACTGTGAGGA TTGGCCTTAT CCAGGGCAAC 
CGAGTGCTCG TTGTCCATGA TGGTTTCGAG GGCCTGGCCA AGGGGCAGAT AGAGGAAGCT 
GGCTGGAGCT ATGTTGGGGG CTGGACTGGC CAAGGTGGCT CTAAACTTGG GACTAAAAGG 
ACTCTACCCA AGAAGAGCTT TGAACAGATC AGTGCCAATA TAACTAAGTT TAACATTCAG 
GGCCTTGTCA TCATTGGGGG CTTTGAGGCT TACACAGGGG GCCTGGAACT GATGGAGGGC 
AGGAAGCAGT TTGATGAGCT CTGCATCCCA TTTGTGGTCA TTCCTGCTAC AGTCTCCAAC 
AATGTCCCTG GCTCAGACTT CAGCGTTGGG GCTGACACAG CACTCAATAC TATCTGCACA 
ACCTGTGACC GCATCAAGCA GTCAGCAGCT GGCACCAAGC GTCGGGTGTT TATCATTGAG 
ACTATGGGTG GCTACTGTGG CTACCTGGCT ACCATGGCTG GACTGGCAGC TGGGGCCGAT 
GCTGCCTACA TTTTTGAGGA GCCCTTCACC ATTCGAGACC TGCAGGCAAA TGTTGAACAT 
CTGGTGCAAA AGATGAAAAC AACTGTGAAA AGGGGCTTGG TGTTAAGGAA TGAAAAGTGC 
AATGAGAACT ATACCACTGA CTTCATTTTC AACCTGTACT CTGAGGAGGG GAAGGGCATC 
TTCGACAGCA GGAAGAATGT GCTTGGTCAC ATGCAGCAGG GTGGGAGCCC AACCCCATTT 
GATAGGAATT TTGCCACTAA GATGGGCGCC AAGGCTATGA ACTGGATGTC TGGGAAAATC 
AAAGAGAGTT ACCGTAATGG GCGGATCTTT GCCAATACTC CAGATTCGGG CTGTGTTCTG 
GGGATGCGTA AGAGGGCTCT GGTCTTCCAA CCAGTGGCTG AGCTGAAGGA CCAGACAGAT 
TTTGAGCATC GAATCCCCAA GGAACAGTGG TGGCTGAAAC TGAGGCCCAT CCTCAAAATC 
CTAGCCAAGT ACGAGATTGA CTTGGACACT TCAGACCATG CCCACCTGGA GCACATCACC 
CGGAAGCGGT CCGGGGAAGC TGCCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350548.1
CDS	186..2435
Translation	MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	NM_001363619.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant 25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363619.1

ORF Insert Sequence:

ATGACCCATG AAGAGCACCA TGCAGCCAAA ACCCTGGGGA TTGGCAAAGC CATTGCTGTC 
TTAACCTCTG GTGGAGATGC CCAAGGTATG AATGCTGCTG TCAGGGCTGT GGTTCGAGTT 
GGTATCTTCA CCGGTGCCCG TGTCTTCTTT GTCCATGAGG GTTATCAAGG CCTGGTGGAT 
GGTGGAGATC ACATCAAGGA AGCCACCTGG GAGAGCGTTT CGATGATGCT TCAGCTGGGA 
GGCACGGTGA TTGGAAGTGC CCGGTGCAAG GACTTTCGGG AACGAGAAGG ACGACTCCGA 
GCTGCCTACA ACCTGGTGAA GCGTGGGATC ACCAATCTCT GTGTCATTGG GGGTGATGGC 
AGCCTCACTG GGGCTGACAC CTTCCGTTCT GAGTGGAGTG ACTTGTTGAG TGACCTCCAG 
AAAGCAGGTA AGATCACAGA TGAGGAGGCT ACGAAGTCCA GCTACCTGAA CATTGTGGGC 
CTGGTTGGGT CAATTGACAA TGACTTCTGT GGCACCGATA TGACCATTGG CACTGACTCT 
GCCCTGCATC GGATCATGGA AATTGTAGAT GCCATCACTA CCACTGCCCA GAGCCACCAG 
AGGACATTTG TGTTAGAAGT AATGGGCCGC CACTGTGGAT ACCTGGCCCT TGTCACCTCT 
CTGTCCTGTG GGGCCGACTG GGTTTTTATT CCTGAATGTC CACCAGATGA CGACTGGGAG 
GAACACCTTT GTCGCCGACT CAGCGAGACA AGGACCCGTG GTTCTCGTCT CAACATCATC 
ATTGTGGCTG AGGGTGCAAT TGACAAGAAT GGAAAACCAA TCACCTCAGA AGACATCAAG 
AATGGCAGCA GGATGGGTGT GGAAGCAGTG ATGGCACTTT TGGAGGGGAC CCCAGATACC 
CCAGCCTGTG TAGTGAGCCT CTCTGGTAAC CAGGCTGTGC GCCTGCCCCT CATGGAATGT 
GTCCAGGTGA CCAAAGATGT GACCAAGGCC ATGGATGAGA AGAAATTTGA CGAAGCCCTG 
AAGCTGAGAG GCCGGAGCTT CATGAACAAC TGGGAGGTGT ACAAGCTTCT AGCTCATGTC 
AGACCCCCGG TATCTAAGAG TGGTTCGCAC ACAGTGGCTG TGATGAACGT GGGGGCTCCG 
GCTGCAGGCA TGAATGCTGC TGTTCGCTCC ACTGTGAGGA TTGGCCTTAT CCAGGGCAAC 
CGAGTGCTCG TTGTCCATGA TGGTTTCGAG GGCCTGGCCA AGGGGCAGAT AGAGGAAGCT 
GGCTGGAGCT ATGTTGGGGG CTGGACTGGC CAAGGTGGCT CTAAACTTGG GACTAAAAGG 
ACTCTACCCA AGAAGAGCTT TGAACAGATC AGTGCCAATA TAACTAAGTT TAACATTCAG 
GGCCTTGTCA TCATTGGGGG CTTTGAGGCT TACACAGGGG GCCTGGAACT GATGGAGGGC 
AGGAAGCAGT TTGATGAGCT CTGCATCCCA TTTGTGGTCA TTCCTGCTAC AGTCTCCAAC 
AATGTCCCTG GCTCAGACTT CAGCGTTGGG GCTGACACAG CACTCAATAC TATCTGCACA 
ACCTGTGACC GCATCAAGCA GTCAGCAGCT GGCACCAAGC GTCGGGTGTT TATCATTGAG 
ACTATGGGTG GCTACTGTGG CTACCTGGCT ACCATGGCTG GACTGGCAGC TGGGGCCGAT 
GCTGCCTACA TTTTTGAGGA GCCCTTCACC ATTCGAGACC TGCAGGCAAA TGTTGAACAT 
CTGGTGCAAA AGATGAAAAC AACTGTGAAA AGGGGCTTGG TGTTAAGGAA TGAAAAGTGC 
AATGAGAACT ATACCACTGA CTTCATTTTC AACCTGTACT CTGAGGAGGG GAAGGGCATC 
TTCGACAGCA GGAAGAATGT GCTTGGTCAC ATGCAGCAGG GTGGGAGCCC AACCCCATTT 
GATAGGAATT TTGCCACTAA GATGGGCGCC AAGGCTATGA ACTGGATGTC TGGGAAAATC 
AAAGAGAGTT ACCGTAATGG GCGGATCTTT GCCAATACTC CAGATTCGGG CTGTGTTCTG 
GGGATGCGTA AGAGGGCTCT GGTCTTCCAA CCAGTGGCTG AGCTGAAGGA CCAGACAGAT 
TTTGAGCATC GAATCCCCAA GGAACAGTGG TGGCTGAAAC TGAGGCCCAT CCTCAAAATC 
CTAGCCAAGT ACGAGATTGA CTTGGACACT TCAGACCATG CCCACCTGGA GCACATCACC 
CGGAAGCGGT CCGGGGAAGC TGCCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_024449020.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304788.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu103737
Clone ID Related Accession (Same CDS sequence)	XM_024449020.1
Accession Version	XM_024449020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAGGG GTTTCCGGCC GGGCGCGGAA CGCAAAACCC GGGAACCGCC GCGAACCGGA 
ACCGCCTTCA CAGCACCGGA AGAGTCGCTA GCTGGGGAAG CTTCTACTTC CAGCATGCTC 
ATACCAAAGC CACCACCAAA GACAGACATC TTGAAGAGTC TAGATACTAT GGATGATCCA 
GACACCGTGG GAAGCATACC TGTTTTCAAA ACTGAGTGGA TCATGACCCA TGAAGAGCAC 
CATGCAGCCA AAACCCTGGG GATTGGCAAA GCCATTGCTG TCTTAACCTC TGGTGGAGAT 
GCCCAAGGTA TGAATGCTGC TGTCAGGGCT GTGGTTCGAG TTGGTATCTT CACCGGTGCC 
CGTGTCTTCT TTGTCCATGA GGGTTATCAA GGCCTGGTGG ATGGTGGAGA TCACATCAAG 
GAAGCCACCT GGGAGAGCGT TTCGATGATG CTTCAGCTGG GAGGCACGGT GATTGGAAGT 
GCCCGGTGCA AGGACTTTCG GGAACGAGAA GGACGACTCC GAGCTGCCTA CAACCTGGTG 
AAGCGTGGGA TCACCAATCT CTGTGTCATT GGGGGTGATG GCAGCCTCAC TGGGGCTGAC 
ACCTTCCGTT CTGAGTGGAG TGACTTGTTG AGTGACCTCC AGAAAGCAGG TAAGATCACA 
GATGAGGAGG CTACGAAGTC CAGCTACCTG AACATTGTGG GCCTGGTTGG GTCAATTGAC 
AATGACTTCT GTGGCACCGA TATGACCATT GGCACTGACT CTGCCCTGCA TCGGATCATG 
GAAATTGTAG ATGCCATCAC TACCACTGCC CAGAGCCACC AGAGGACATT TGTGTTAGAA 
GTAATGGGCC GCCACTGTGG ATACCTGGCC CTTGTCACCT CTCTGTCCTG TGGGGCCGAC 
TGGGTTTTTA TTCCTGAATG TCCACCAGAT GACGACTGGG AGGAACACCT TTGTCGCCGA 
CTCAGCGAGA CAAGGACCCG TGGTTCTCGT CTCAACATCA TCATTGTGGC TGAGGGTGCA 
ATTGACAAGA ATGGAAAACC AATCACCTCA GAAGACATCA AGAATCTGGT GGTTAAGCGT 
CTGGGATATG ACACCCGGGT TACTGTCTTG GGGCATGTGC AGAGGGGTGG GACGCCATCA 
GCCTTTGACA GAATTCTGGG CAGCAGGATG GGTGTGGAAG CAGTGATGGC ACTTTTGGAG 
GGGACCCCAG ATACCCCAGC CTGTGTAGTG AGCCTCTCTG GTAACCAGGC TGTGCGCCTG 
CCCCTCATGG AATGTGTCCA GGTGACCAAA GATGTGACCA AGGCCATGGA TGAGAAGAAA 
TTTGACGAAG CCCTGAAGCT GAGAGGCCGG AGCTTCATGA ACAACTGGGA GGTGTACAAG 
CTTCTAGCTC ATGTCAGACC CCCGGTATCT AAGAGTGGTT CGCACACAGT GGCTGTGATG 
AACGTGGGGG CTCCGGCTGC AGGCATGAAT GCTGCTGTTC GCTCCACTGT GAGGATTGGC 
CTTATCCAGG GCAACCGAGT GCTCGTTGTC CATGATGGTT TCGAGGGCCT GGCCAAGGGG 
CAGATAGAGG AAGCTGGCTG GAGCTATGTT GGGGGCTGGA CTGGCCAAGG TGGCTCTAAA 
CTTGGGACTA AAAGGACTCT ACCCAAGAAG AGCTTTGAAC AGATCAGTGC CAATATAACT 
AAGTTTAACA TTCAGGGCCT TGTCATCATT GGGGGCTTTG AGGCTTACAC AGGGGGCCTG 
GAACTGATGG AGGGCAGGAA GCAGTTTGAT GAGCTCTGCA TCCCATTTGT GGTCATTCCT 
GCTACAGTCT CCAACAATGT CCCTGGCTCA GACTTCAGCG TTGGGGCTGA CACAGCACTC 
AATACTATCT GCACAACCTG TGACCGCATC AAGCAGTCAG CAGCTGGCAC CAAGCGTCGG 
GTGTTTATCA TTGAGACTAT GGGTGGCTAC TGTGGCTACC TGGCTACCAT GGCTGGACTG 
GCAGCTGGGG CCGATGCTGC CTACATTTTT GAGGAGCCCT TCACCATTCG AGACCTGCAG 
GCAAATGTTG AACATCTGGT GCAAAAGATG AAAACAACTG TGAAAAGGGG CTTGGTGTTA 
AGGAATGAAA AGTGCAATGA GAACTATACC ACTGACTTCA TTTTCAACCT GTACTCTGAG 
GAGGGGAAGG GCATCTTCGA CAGCAGGAAG AATGTGCTTG GTCACATGCA GCAGGGTGGG 
AGCCCAACCC CATTTGATAG GAATTTTGCC ACTAAGATGG GCGCCAAGGC TATGAACTGG 
ATGTCTGGGA AAATCAAAGA GAGTTACCGT AATGGGCGGA TCTTTGCCAA TACTCCAGAT 
TCGGGCTGTG TTCTGGGGAT GCGTAAGAGG GCTCTGGTCT TCCAACCAGT GGCTGAGCTG 
AAGGACCAGA CAGATTTTGA GCATCGAATC CCCAAGGAAC AGTGGTGGCT GAAACTGAGG 
CCCATCCTCA AAATCCTAGC CAAGTACGAG ATTGACTTGG ACACTTCAGA CCATGCCCAC 
CTGGAGCACA TCACCCGGAA GCGGTCCGGG GAAGCTGCCG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304788.1
CDS	125..2689
Translation	MRRGFRPGAERKTREPPRTGTAFTAPEESLAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_024449020.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449020.1

ORF Insert Sequence:

ATGCGAAGGG GTTTCCGGCC GGGCGCGGAA CGCAAAACCC GGGAACCGCC GCGAACCGGA 
ACCGCCTTCA CAGCACCGGA AGAGTCGCTA GCTGGGGAAG CTTCTACTTC CAGCATGCTC 
ATACCAAAGC CACCACCAAA GACAGACATC TTGAAGAGTC TAGATACTAT GGATGATCCA 
GACACCGTGG GAAGCATACC TGTTTTCAAA ACTGAGTGGA TCATGACCCA TGAAGAGCAC 
CATGCAGCCA AAACCCTGGG GATTGGCAAA GCCATTGCTG TCTTAACCTC TGGTGGAGAT 
GCCCAAGGTA TGAATGCTGC TGTCAGGGCT GTGGTTCGAG TTGGTATCTT CACCGGTGCC 
CGTGTCTTCT TTGTCCATGA GGGTTATCAA GGCCTGGTGG ATGGTGGAGA TCACATCAAG 
GAAGCCACCT GGGAGAGCGT TTCGATGATG CTTCAGCTGG GAGGCACGGT GATTGGAAGT 
GCCCGGTGCA AGGACTTTCG GGAACGAGAA GGACGACTCC GAGCTGCCTA CAACCTGGTG 
AAGCGTGGGA TCACCAATCT CTGTGTCATT GGGGGTGATG GCAGCCTCAC TGGGGCTGAC 
ACCTTCCGTT CTGAGTGGAG TGACTTGTTG AGTGACCTCC AGAAAGCAGG TAAGATCACA 
GATGAGGAGG CTACGAAGTC CAGCTACCTG AACATTGTGG GCCTGGTTGG GTCAATTGAC 
AATGACTTCT GTGGCACCGA TATGACCATT GGCACTGACT CTGCCCTGCA TCGGATCATG 
GAAATTGTAG ATGCCATCAC TACCACTGCC CAGAGCCACC AGAGGACATT TGTGTTAGAA 
GTAATGGGCC GCCACTGTGG ATACCTGGCC CTTGTCACCT CTCTGTCCTG TGGGGCCGAC 
TGGGTTTTTA TTCCTGAATG TCCACCAGAT GACGACTGGG AGGAACACCT TTGTCGCCGA 
CTCAGCGAGA CAAGGACCCG TGGTTCTCGT CTCAACATCA TCATTGTGGC TGAGGGTGCA 
ATTGACAAGA ATGGAAAACC AATCACCTCA GAAGACATCA AGAATCTGGT GGTTAAGCGT 
CTGGGATATG ACACCCGGGT TACTGTCTTG GGGCATGTGC AGAGGGGTGG GACGCCATCA 
GCCTTTGACA GAATTCTGGG CAGCAGGATG GGTGTGGAAG CAGTGATGGC ACTTTTGGAG 
GGGACCCCAG ATACCCCAGC CTGTGTAGTG AGCCTCTCTG GTAACCAGGC TGTGCGCCTG 
CCCCTCATGG AATGTGTCCA GGTGACCAAA GATGTGACCA AGGCCATGGA TGAGAAGAAA 
TTTGACGAAG CCCTGAAGCT GAGAGGCCGG AGCTTCATGA ACAACTGGGA GGTGTACAAG 
CTTCTAGCTC ATGTCAGACC CCCGGTATCT AAGAGTGGTT CGCACACAGT GGCTGTGATG 
AACGTGGGGG CTCCGGCTGC AGGCATGAAT GCTGCTGTTC GCTCCACTGT GAGGATTGGC 
CTTATCCAGG GCAACCGAGT GCTCGTTGTC CATGATGGTT TCGAGGGCCT GGCCAAGGGG 
CAGATAGAGG AAGCTGGCTG GAGCTATGTT GGGGGCTGGA CTGGCCAAGG TGGCTCTAAA 
CTTGGGACTA AAAGGACTCT ACCCAAGAAG AGCTTTGAAC AGATCAGTGC CAATATAACT 
AAGTTTAACA TTCAGGGCCT TGTCATCATT GGGGGCTTTG AGGCTTACAC AGGGGGCCTG 
GAACTGATGG AGGGCAGGAA GCAGTTTGAT GAGCTCTGCA TCCCATTTGT GGTCATTCCT 
GCTACAGTCT CCAACAATGT CCCTGGCTCA GACTTCAGCG TTGGGGCTGA CACAGCACTC 
AATACTATCT GCACAACCTG TGACCGCATC AAGCAGTCAG CAGCTGGCAC CAAGCGTCGG 
GTGTTTATCA TTGAGACTAT GGGTGGCTAC TGTGGCTACC TGGCTACCAT GGCTGGACTG 
GCAGCTGGGG CCGATGCTGC CTACATTTTT GAGGAGCCCT TCACCATTCG AGACCTGCAG 
GCAAATGTTG AACATCTGGT GCAAAAGATG AAAACAACTG TGAAAAGGGG CTTGGTGTTA 
AGGAATGAAA AGTGCAATGA GAACTATACC ACTGACTTCA TTTTCAACCT GTACTCTGAG 
GAGGGGAAGG GCATCTTCGA CAGCAGGAAG AATGTGCTTG GTCACATGCA GCAGGGTGGG 
AGCCCAACCC CATTTGATAG GAATTTTGCC ACTAAGATGG GCGCCAAGGC TATGAACTGG 
ATGTCTGGGA AAATCAAAGA GAGTTACCGT AATGGGCGGA TCTTTGCCAA TACTCCAGAT 
TCGGGCTGTG TTCTGGGGAT GCGTAAGAGG GCTCTGGTCT TCCAACCAGT GGCTGAGCTG 
AAGGACCAGA CAGATTTTGA GCATCGAATC CCCAAGGAAC AGTGGTGGCT GAAACTGAGG 
CCCATCCTCA AAATCCTAGC CAAGTACGAG ATTGACTTGG ACACTTCAGA CCATGCCCAC 
CTGGAGCACA TCACCCGGAA GCGGTCCGGG GAAGCTGCCG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_024449021.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304789.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu103738
Clone ID Related Accession (Same CDS sequence)	XM_024449021.1
Accession Version	XM_024449021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAAGGG GTTTCCGGCC GGGCGCGGAA CGCAAAACCC GGGAACCGCC GCGAACCGGA 
ACCGCCTTCA CAGCACCGGA AGAGTCGCTA GAGTGGATCA TGACCCATGA AGAGCACCAT 
GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC ATTGCTGTCT TAACCTCTGG TGGAGATGCC 
CAAGGTATGA ATGCTGCTGT CAGGGCTGTG GTTCGAGTTG GTATCTTCAC CGGTGCCCGT 
GTCTTCTTTG TCCATGAGGG TTATCAAGGC CTGGTGGATG GTGGAGATCA CATCAAGGAA 
GCCACCTGGG AGAGCGTTTC GATGATGCTT CAGCTGGGAG GCACGGTGAT TGGAAGTGCC 
CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA CGACTCCGAG CTGCCTACAA CCTGGTGAAG 
CGTGGGATCA CCAATCTCTG TGTCATTGGG GGTGATGGCA GCCTCACTGG GGCTGACACC 
TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT GACCTCCAGA AAGCAGGTAA GATCACAGAT 
GAGGAGGCTA CGAAGTCCAG CTACCTGAAC ATTGTGGGCC TGGTTGGGTC AATTGACAAT 
GACTTCTGTG GCACCGATAT GACCATTGGC ACTGACTCTG CCCTGCATCG GATCATGGAA 
ATTGTAGATG CCATCACTAC CACTGCCCAG AGCCACCAGA GGACATTTGT GTTAGAAGTA 
ATGGGCCGCC ACTGTGGATA CCTGGCCCTT GTCACCTCTC TGTCCTGTGG GGCCGACTGG 
GTTTTTATTC CTGAATGTCC ACCAGATGAC GACTGGGAGG AACACCTTTG TCGCCGACTC 
AGCGAGACAA GGACCCGTGG TTCTCGTCTC AACATCATCA TTGTGGCTGA GGGTGCAATT 
GACAAGAATG GAAAACCAAT CACCTCAGAA GACATCAAGA ATCTGGTGGT TAAGCGTCTG 
GGATATGACA CCCGGGTTAC TGTCTTGGGG CATGTGCAGA GGGGTGGGAC GCCATCAGCC 
TTTGACAGAA TTCTGGGCAG CAGGATGGGT GTGGAAGCAG TGATGGCACT TTTGGAGGGG 
ACCCCAGATA CCCCAGCCTG TGTAGTGAGC CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC 
CTCATGGAAT GTGTCCAGGT GACCAAAGAT GTGACCAAGG CCATGGATGA GAAGAAATTT 
GACGAAGCCC TGAAGCTGAG AGGCCGGAGC TTCATGAACA ACTGGGAGGT GTACAAGCTT 
CTAGCTCATG TCAGACCCCC GGTATCTAAG AGTGGTTCGC ACACAGTGGC TGTGATGAAC 
GTGGGGGCTC CGGCTGCAGG CATGAATGCT GCTGTTCGCT CCACTGTGAG GATTGGCCTT 
ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT GATGGTTTCG AGGGCCTGGC CAAGGGGCAG 
ATAGAGGAAG CTGGCTGGAG CTATGTTGGG GGCTGGACTG GCCAAGGTGG CTCTAAACTT 
GGGACTAAAA GGACTCTACC CAAGAAGAGC TTTGAACAGA TCAGTGCCAA TATAACTAAG 
TTTAACATTC AGGGCCTTGT CATCATTGGG GGCTTTGAGG CTTACACAGG GGGCCTGGAA 
CTGATGGAGG GCAGGAAGCA GTTTGATGAG CTCTGCATCC CATTTGTGGT CATTCCTGCT 
ACAGTCTCCA ACAATGTCCC TGGCTCAGAC TTCAGCGTTG GGGCTGACAC AGCACTCAAT 
ACTATCTGCA CAACCTGTGA CCGCATCAAG CAGTCAGCAG CTGGCACCAA GCGTCGGGTG 
TTTATCATTG AGACTATGGG TGGCTACTGT GGCTACCTGG CTACCATGGC TGGACTGGCA 
GCTGGGGCCG ATGCTGCCTA CATTTTTGAG GAGCCCTTCA CCATTCGAGA CCTGCAGGCA 
AATGTTGAAC ATCTGGTGCA AAAGATGAAA ACAACTGTGA AAAGGGGCTT GGTGTTAAGG 
AATGAAAAGT GCAATGAGAA CTATACCACT GACTTCATTT TCAACCTGTA CTCTGAGGAG 
GGGAAGGGCA TCTTCGACAG CAGGAAGAAT GTGCTTGGTC ACATGCAGCA GGGTGGGAGC 
CCAACCCCAT TTGATAGGAA TTTTGCCACT AAGATGGGCG CCAAGGCTAT GAACTGGATG 
TCTGGGAAAA TCAAAGAGAG TTACCGTAAT GGGCGGATCT TTGCCAATAC TCCAGATTCG 
GGCTGTGTTC TGGGGATGCG TAAGAGGGCT CTGGTCTTCC AACCAGTGGC TGAGCTGAAG 
GACCAGACAG ATTTTGAGCA TCGAATCCCC AAGGAACAGT GGTGGCTGAA ACTGAGGCCC 
ATCCTCAAAA TCCTAGCCAA GTACGAGATT GACTTGGACA CTTCAGACCA TGCCCACCTG 
GAGCACATCA CCCGGAAGCG GTCCGGGGAA GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304789.1
CDS	137..2578
Translation	MRRGFRPGAERKTREPPRTGTAFTAPEESLEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_024449021.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449021.1

ORF Insert Sequence:

ATGCGAAGGG GTTTCCGGCC GGGCGCGGAA CGCAAAACCC GGGAACCGCC GCGAACCGGA 
ACCGCCTTCA CAGCACCGGA AGAGTCGCTA GAGTGGATCA TGACCCATGA AGAGCACCAT 
GCAGCCAAAA CCCTGGGGAT TGGCAAAGCC ATTGCTGTCT TAACCTCTGG TGGAGATGCC 
CAAGGTATGA ATGCTGCTGT CAGGGCTGTG GTTCGAGTTG GTATCTTCAC CGGTGCCCGT 
GTCTTCTTTG TCCATGAGGG TTATCAAGGC CTGGTGGATG GTGGAGATCA CATCAAGGAA 
GCCACCTGGG AGAGCGTTTC GATGATGCTT CAGCTGGGAG GCACGGTGAT TGGAAGTGCC 
CGGTGCAAGG ACTTTCGGGA ACGAGAAGGA CGACTCCGAG CTGCCTACAA CCTGGTGAAG 
CGTGGGATCA CCAATCTCTG TGTCATTGGG GGTGATGGCA GCCTCACTGG GGCTGACACC 
TTCCGTTCTG AGTGGAGTGA CTTGTTGAGT GACCTCCAGA AAGCAGGTAA GATCACAGAT 
GAGGAGGCTA CGAAGTCCAG CTACCTGAAC ATTGTGGGCC TGGTTGGGTC AATTGACAAT 
GACTTCTGTG GCACCGATAT GACCATTGGC ACTGACTCTG CCCTGCATCG GATCATGGAA 
ATTGTAGATG CCATCACTAC CACTGCCCAG AGCCACCAGA GGACATTTGT GTTAGAAGTA 
ATGGGCCGCC ACTGTGGATA CCTGGCCCTT GTCACCTCTC TGTCCTGTGG GGCCGACTGG 
GTTTTTATTC CTGAATGTCC ACCAGATGAC GACTGGGAGG AACACCTTTG TCGCCGACTC 
AGCGAGACAA GGACCCGTGG TTCTCGTCTC AACATCATCA TTGTGGCTGA GGGTGCAATT 
GACAAGAATG GAAAACCAAT CACCTCAGAA GACATCAAGA ATCTGGTGGT TAAGCGTCTG 
GGATATGACA CCCGGGTTAC TGTCTTGGGG CATGTGCAGA GGGGTGGGAC GCCATCAGCC 
TTTGACAGAA TTCTGGGCAG CAGGATGGGT GTGGAAGCAG TGATGGCACT TTTGGAGGGG 
ACCCCAGATA CCCCAGCCTG TGTAGTGAGC CTCTCTGGTA ACCAGGCTGT GCGCCTGCCC 
CTCATGGAAT GTGTCCAGGT GACCAAAGAT GTGACCAAGG CCATGGATGA GAAGAAATTT 
GACGAAGCCC TGAAGCTGAG AGGCCGGAGC TTCATGAACA ACTGGGAGGT GTACAAGCTT 
CTAGCTCATG TCAGACCCCC GGTATCTAAG AGTGGTTCGC ACACAGTGGC TGTGATGAAC 
GTGGGGGCTC CGGCTGCAGG CATGAATGCT GCTGTTCGCT CCACTGTGAG GATTGGCCTT 
ATCCAGGGCA ACCGAGTGCT CGTTGTCCAT GATGGTTTCG AGGGCCTGGC CAAGGGGCAG 
ATAGAGGAAG CTGGCTGGAG CTATGTTGGG GGCTGGACTG GCCAAGGTGG CTCTAAACTT 
GGGACTAAAA GGACTCTACC CAAGAAGAGC TTTGAACAGA TCAGTGCCAA TATAACTAAG 
TTTAACATTC AGGGCCTTGT CATCATTGGG GGCTTTGAGG CTTACACAGG GGGCCTGGAA 
CTGATGGAGG GCAGGAAGCA GTTTGATGAG CTCTGCATCC CATTTGTGGT CATTCCTGCT 
ACAGTCTCCA ACAATGTCCC TGGCTCAGAC TTCAGCGTTG GGGCTGACAC AGCACTCAAT 
ACTATCTGCA CAACCTGTGA CCGCATCAAG CAGTCAGCAG CTGGCACCAA GCGTCGGGTG 
TTTATCATTG AGACTATGGG TGGCTACTGT GGCTACCTGG CTACCATGGC TGGACTGGCA 
GCTGGGGCCG ATGCTGCCTA CATTTTTGAG GAGCCCTTCA CCATTCGAGA CCTGCAGGCA 
AATGTTGAAC ATCTGGTGCA AAAGATGAAA ACAACTGTGA AAAGGGGCTT GGTGTTAAGG 
AATGAAAAGT GCAATGAGAA CTATACCACT GACTTCATTT TCAACCTGTA CTCTGAGGAG 
GGGAAGGGCA TCTTCGACAG CAGGAAGAAT GTGCTTGGTC ACATGCAGCA GGGTGGGAGC 
CCAACCCCAT TTGATAGGAA TTTTGCCACT AAGATGGGCG CCAAGGCTAT GAACTGGATG 
TCTGGGAAAA TCAAAGAGAG TTACCGTAAT GGGCGGATCT TTGCCAATAC TCCAGATTCG 
GGCTGTGTTC TGGGGATGCG TAAGAGGGCT CTGGTCTTCC AACCAGTGGC TGAGCTGAAG 
GACCAGACAG ATTTTGAGCA TCGAATCCCC AAGGAACAGT GGTGGCTGAA ACTGAGGCCC 
ATCCTCAAAA TCCTAGCCAA GTACGAGATT GACTTGGACA CTTCAGACCA TGCCCACCTG 
GAGCACATCA CCCGGAAGCG GTCCGGGGAA GCTGCCGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKM ( XM_017019469.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874958.1 Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X6, mRNA.

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CloneID	OHu83887
Clone ID Related Accession (Same CDS sequence)	XM_017019469.1
Accession Version	XM_017019469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ATP-dependent 6-phosphofructokinase, muscle type isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874958.1
CDS	158..2620
Translation	MHKDEFHLKFFMCVIQSRQLVRTPQRTAGEASTSSMLIPKPPPKTDILKSLDTMDDPDTVGSIPVFKTEWIMTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGDHIKEATWESVSMMLQLGGTVIGSARCKDFREREGRLRAAYNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLSDLQKAGKITDEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIMEIVDAITTTAQSHQRTFVLEVMGRHCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIKNGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVTKAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHITRKRSGEAAV

Target ORF information:

RefSeq Version	XM_017019469.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphofructokinase, muscle (PFKM), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019469.1

ORF Insert Sequence:

ATGCATAAAG ACGAGTTTCA TCTGAAATTT TTCATGTGTG TGATTCAGTC TCGCCAGTTA 
GTCAGGACTC CTCAGAGAAC AGCTGGGGAA GCTTCTACTT CCAGCATGCT CATACCAAAG 
CCACCACCAA AGACAGACAT CTTGAAGAGT CTAGATACTA TGGATGATCC AGACACCGTG 
GGAAGCATAC CTGTTTTCAA AACTGAGTGG ATCATGACCC ATGAAGAGCA CCATGCAGCC 
AAAACCCTGG GGATTGGCAA AGCCATTGCT GTCTTAACCT CTGGTGGAGA TGCCCAAGGT 
ATGAATGCTG CTGTCAGGGC TGTGGTTCGA GTTGGTATCT TCACCGGTGC CCGTGTCTTC 
TTTGTCCATG AGGGTTATCA AGGCCTGGTG GATGGTGGAG ATCACATCAA GGAAGCCACC 
TGGGAGAGCG TTTCGATGAT GCTTCAGCTG GGAGGCACGG TGATTGGAAG TGCCCGGTGC 
AAGGACTTTC GGGAACGAGA AGGACGACTC CGAGCTGCCT ACAACCTGGT GAAGCGTGGG 
ATCACCAATC TCTGTGTCAT TGGGGGTGAT GGCAGCCTCA CTGGGGCTGA CACCTTCCGT 
TCTGAGTGGA GTGACTTGTT GAGTGACCTC CAGAAAGCAG GTAAGATCAC AGATGAGGAG 
GCTACGAAGT CCAGCTACCT GAACATTGTG GGCCTGGTTG GGTCAATTGA CAATGACTTC 
TGTGGCACCG ATATGACCAT TGGCACTGAC TCTGCCCTGC ATCGGATCAT GGAAATTGTA 
GATGCCATCA CTACCACTGC CCAGAGCCAC CAGAGGACAT TTGTGTTAGA AGTAATGGGC 
CGCCACTGTG GATACCTGGC CCTTGTCACC TCTCTGTCCT GTGGGGCCGA CTGGGTTTTT 
ATTCCTGAAT GTCCACCAGA TGACGACTGG GAGGAACACC TTTGTCGCCG ACTCAGCGAG 
ACAAGGACCC GTGGTTCTCG TCTCAACATC ATCATTGTGG CTGAGGGTGC AATTGACAAG 
AATGGAAAAC CAATCACCTC AGAAGACATC AAGAATGGCA GCAGGATGGG TGTGGAAGCA 
GTGATGGCAC TTTTGGAGGG GACCCCAGAT ACCCCAGCCT GTGTAGTGAG CCTCTCTGGT 
AACCAGGCTG TGCGCCTGCC CCTCATGGAA TGTGTCCAGG TGACCAAAGA TGTGACCAAG 
GCCATGGATG AGAAGAAATT TGACGAAGCC CTGAAGCTGA GAGGCCGGAG CTTCATGAAC 
AACTGGGAGG TGTACAAGCT TCTAGCTCAT GTCAGACCCC CGGTATCTAA GAGTGGTTCG 
CACACAGTGG CTGTGATGAA CGTGGGGGCT CCGGCTGCAG GCATGAATGC TGCTGTTCGC 
TCCACTGTGA GGATTGGCCT TATCCAGGGC AACCGAGTGC TCGTTGTCCA TGATGGTTTC 
GAGGGCCTGG CCAAGGGGCA GATAGAGGAA GCTGGCTGGA GCTATGTTGG GGGCTGGACT 
GGCCAAGGTG GCTCTAAACT TGGGACTAAA AGGACTCTAC CCAAGAAGAG CTTTGAACAG 
ATCAGTGCCA ATATAACTAA GTTTAACATT CAGGGCCTTG TCATCATTGG GGGCTTTGAG 
GCTTACACAG GGGGCCTGGA ACTGATGGAG GGCAGGAAGC AGTTTGATGA GCTCTGCATC 
CCATTTGTGG TCATTCCTGC TACAGTCTCC AACAATGTCC CTGGCTCAGA CTTCAGCGTT 
GGGGCTGACA CAGCACTCAA TACTATCTGC ACAACCTGTG ACCGCATCAA GCAGTCAGCA 
GCTGGCACCA AGCGTCGGGT GTTTATCATT GAGACTATGG GTGGCTACTG TGGCTACCTG 
GCTACCATGG CTGGACTGGC AGCTGGGGCC GATGCTGCCT ACATTTTTGA GGAGCCCTTC 
ACCATTCGAG ACCTGCAGGC AAATGTTGAA CATCTGGTGC AAAAGATGAA AACAACTGTG 
AAAAGGGGCT TGGTGTTAAG GAATGAAAAG TGCAATGAGA ACTATACCAC TGACTTCATT 
TTCAACCTGT ACTCTGAGGA GGGGAAGGGC ATCTTCGACA GCAGGAAGAA TGTGCTTGGT 
CACATGCAGC AGGGTGGGAG CCCAACCCCA TTTGATAGGA ATTTTGCCAC TAAGATGGGC 
GCCAAGGCTA TGAACTGGAT GTCTGGGAAA ATCAAAGAGA GTTACCGTAA TGGGCGGATC 
TTTGCCAATA CTCCAGATTC GGGCTGTGTT CTGGGGATGC GTAAGAGGGC TCTGGTCTTC 
CAACCAGTGG CTGAGCTGAA GGACCAGACA GATTTTGAGC ATCGAATCCC CAAGGAACAG 
TGGTGGCTGA AACTGAGGCC CATCCTCAAA ATCCTAGCCA AGTACGAGAT TGACTTGGAC 
ACTTCAGACC ATGCCCACCT GGAGCACATC ACCCGGAAGC GGTCCGGGGA AGCTGCCGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The glycolytic enzyme phosphofructokinase-1 assembles into filaments.
The Journal of cell biology216(8)2305-2313(2017 Aug)
Webb BA,Dosey AM,Wittmann T,Kollman JM,Barber DL

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

The expression pattern of PFKFB3 enzyme distinguishes between induced-pluripotent stem cells and cancer stem cells.
Oncotarget6(30)29753-70(2015 Oct)
Cie?lar-Pobuda A,Jain MV,Kratz G,Rzeszowska-Wolny J,Ghavami S,Wiechec E

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PFKM cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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