TDP2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TDP2
Entrez Gene ID	51567
Full Name	tyrosyl-DNA phosphodiesterase 2
Synonyms	AD022,EAP2,EAPII,TTRAP,dJ30M3.3,hTDP2
General protein information	Preferred Names tyrosyl-DNA phosphodiesterase 2 Names tyrosyl-DNA phosphodiesterase 2 5'-Tyr-DNA phosphodiesterase 5'-tyrosyl-DNA phosphodiesterase ETS1-associated protein 2 ETS1-associated protein II TRAF and TNF receptor-associated protein VPg unlinkase tyr-DNA phosphodiesterase 2 tyrosyl-RNA phosphodiesterase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p22.3
Summary	This gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. [provided by RefSeq, Jul 2008].
Disorder MIM:	605764
Disorder Html:	Spinocerebellar ataxia, autosomal recessive 23, 616949 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_016614.3	NP_057698.2	tyrosyl-DNA phosphodiesterase 2
NM_016614.2	NP_057698.2	tyrosyl-DNA phosphodiesterase 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TDP2	XP_005640219.1
Mus musculus (house mouse)	Tdp2	NP_062424.1
Gallus gallus (chicken)	TDP2	NP_001263313.1
	TDP2	XP_001105245.2
Danio rerio (zebrafish)	tdp2b	NP_001073171.1
Xenopus tropicalis (tropical clawed frog)	tdp2	NP_001016944.1
Bos taurus (cattle)	TDP2	NP_001098811.1
Rattus norvegicus (Norway rat)	Tdp2	NP_001030119.1
Homo sapiens (human)	TDP2	NP_057698.2
Pan troglodytes (chimpanzee)	TDP2	XP_518271.3
Caenorhabditis elegans (roundworm)	CELE_Y63D3A.4	NP_493461.1

Gene Ontology
Bibliography

Related articles in PubMed

VPg unlinkase/TDP2 in cardiovirus infected cells: Re-localization and proteolytic cleavage.
Maciejewski S, Ullmer W, Semler BL
Virology516139-146(2018 Jan)

Tyrosyl-DNA phosphodiesterases: rescuing the genome from the risks of relaxation.
Kawale AS, Povirk LF
Nucleic acids research(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TDP2 suppresses chromosome rearrangements induced by TOP2 and reduces TOP2-induced chromosome translocations that arise during gene transcription.
Title: TDP2 suppresses chromosomal translocations induced by DNA topoisomerase II during gene transcription.

Both the genome instability and cell death of MRE11-null and MRE11-mutated H129N cells are significantly reversed by overexpression of Tdp2, an enzyme that eliminates covalent Top2 conjugates; thus, the essential role of Mre11 nuclease activity is likely to remove the DNA lesions.
Title: Mre11 Is Essential for the Removal of Lethal Topoisomerase 2 Covalent Cleavage Complexes.

Study revealed a post-translational regulation of TDP2 activity and discovered a new role of ERK3 in increasing cancer cells' DNA damage response and chemoresistance to Top2 inhibitors.
Title: ERK3 regulates TDP2-mediated DNA damage response and chemoresistance in lung cancer cells.

Data show that DNA-repair enzyme TDP2 knockdown in HepG2 cells significantly delays the conversion of relaxed circular DNA (RC-DNA) to covalently closed circular (ccc) DNA.
Title: Involvement of the host DNA-repair enzyme TDP2 in formation of the covalently closed circular DNA persistence reservoir of hepatitis B viruses.

This article summarizes and compares the biochemistry, functions, and post-translational regulation of TDP1 and TDP2, as well as the relevance of TDP1 and TDP2 as determinants of response to anticancer agents
Title: Tyrosyl-DNA-phosphodiesterases (TDP1 and TDP2).

The following TDP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TDP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13222	NM_016614.3 Latest version!	Homo sapiens tyrosyl-DNA phosphodiesterase 2 (TDP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu13222	NM_016614.2	Homo sapiens tyrosyl-DNA phosphodiesterase 2 (TDP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TDP2 ( NM_016614.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057698.2 Homo sapiens tyrosyl-DNA phosphodiesterase 2 (TDP2), mRNA.

Price & Availability↑

CloneID	OHu13222
Clone ID Related Accession (Same CDS sequence)	NM_016614.3 , NM_016614.2
Accession Version	NM_016614.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	tyrosyl-DNA phosphodiesterase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL031775.1. This sequence is a reference standard in the RefSeqGene project. On Nov 26, 2018 this sequence version replaced NM_016614.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.245659.1, SRR3476690.37100.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000378198.9/ ENSP00000367440.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGTTGG GGAGTTGCCT GGAGGGCGGG AGGGAGGCGG CGGAGGAAGA GGGCGAGCCT 
GAGGTGAAAA AGCGGCGACT TCTGTGTGTG GAGTTTGCCT CGGTCGCAAG CTGCGATGCC 
GCAGTGGCTC AGTGCTTCCT GGCCGAGAAC GACTGGGAGA TGGAAAGGGC TCTGAACTCC 
TACTTCGAGC CTCCGGTGGA GGAGAGCGCC TTGGAACGCC GACCTGAAAC CATCTCTGAG 
CCCAAGACCT ATGTTGACCT AACCAATGAA GAAACAACTG ATTCCACCAC TTCTAAAATC 
AGCCCATCTG AAGATACTCA GCAAGAAAAT GGCAGCATGT TCTCTCTCAT TACCTGGAAT 
ATTGATGGAT TAGATCTAAA CAATCTGTCA GAGAGGGCTC GAGGGGTGTG TTCCTACTTA 
GCTTTGTACA GCCCAGATGT GATATTTCTA CAGGAAGTTA TTCCCCCATA TTATAGCTAC 
CTAAAGAAGA GATCAAGTAA TTATGAGATT ATTACAGGTC ATGAAGAAGG ATATTTCACA 
GCTATAATGT TGAAGAAATC AAGAGTGAAA TTAAAAAGCC AAGAGATTAT TCCTTTTCCA 
AGTACCAAAA TGATGAGAAA CCTTTTATGT GTGCATGTGA ACGTGTCAGG AAATGAGCTT 
TGCCTTATGA CATCCCATTT GGAGAGCACC AGAGGGCATG CTGCGGAACG AATGAATCAG 
TTAAAAATGG TTTTAAAGAA AATGCAAGAG GCTCCAGAGT CAGCTACAGT TATATTTGCA 
GGAGATACAA ATCTAAGGGA TCGAGAGGTT ACCAGATGTG GTGGTTTACC CAACAACATT 
GTGGATGTCT GGGAGTTTTT GGGCAAACCT AAACATTGCC AGTATACATG GGATACACAA 
ATGAACTCTA ATCTTGGAAT AACTGCTGCT TGTAAACTTC GTTTTGATCG AATATTTTTC 
AGAGCAGCAG CAGAAGAGGG ACACATTATT CCCCGAAGTT TGGACCTTCT TGGATTAGAA 
AAACTGGACT GTGGTAGATT TCCTAGTGAT CACTGGGGTC TTCTGTGCAA CTTAGATATA 
ATATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057698.2
CDS	38..1126
Translation	MELGSCLEGGREAAEEEGEPEVKKRRLLCVEFASVASCDAAVAQCFLAENDWEMERALNSYFEPPVEESALERRPETISEPKTYVDLTNEETTDSTTSKISPSEDTQQENGSMFSLITWNIDGLDLNNLSERARGVCSYLALYSPDVIFLQEVIPPYYSYLKKRSSNYEIITGHEEGYFTAIMLKKSRVKLKSQEIIPFPSTKMMRNLLCVHVNVSGNELCLMTSHLESTRGHAAERMNQLKMVLKKMQEAPESATVIFAGDTNLRDREVTRCGGLPNNIVDVWEFLGKPKHCQYTWDTQMNSNLGITAACKLRFDRIFFRAAAEEGHIIPRSLDLLGLEKLDCGRFPSDHWGLLCNLDIIL

Target ORF information:

RefSeq Version	NM_016614.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tyrosyl-DNA phosphodiesterase 2 (TDP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016614.3

ORF Insert Sequence:

ATGGAGTTGG GGAGTTGCCT GGAGGGCGGG AGGGAGGCGG CGGAGGAAGA GGGCGAGCCT 
GAGGTGAAAA AGCGGCGACT TCTGTGTGTG GAGTTTGCCT CGGTCGCAAG CTGCGATGCC 
GCAGTGGCTC AGTGCTTCCT GGCCGAGAAC GACTGGGAGA TGGAAAGGGC TCTGAACTCC 
TACTTCGAGC CTCCGGTGGA GGAGAGCGCC TTGGAACGCC GACCTGAAAC CATCTCTGAG 
CCCAAGACCT ATGTTGACCT AACCAATGAA GAAACAACTG ATTCCACCAC TTCTAAAATC 
AGCCCATCTG AAGATACTCA GCAAGAAAAT GGCAGCATGT TCTCTCTCAT TACCTGGAAT 
ATTGATGGAT TAGATCTAAA CAATCTGTCA GAGAGGGCTC GAGGGGTGTG TTCCTACTTA 
GCTTTGTACA GCCCAGATGT GATATTTCTA CAGGAAGTTA TTCCCCCATA TTATAGCTAC 
CTAAAGAAGA GATCAAGTAA TTATGAGATT ATTACAGGTC ATGAAGAAGG ATATTTCACA 
GCTATAATGT TGAAGAAATC AAGAGTGAAA TTAAAAAGCC AAGAGATTAT TCCTTTTCCA 
AGTACCAAAA TGATGAGAAA CCTTTTATGT GTGCATGTGA ACGTGTCAGG AAATGAGCTT 
TGCCTTATGA CATCCCATTT GGAGAGCACC AGAGGGCATG CTGCGGAACG AATGAATCAG 
TTAAAAATGG TTTTAAAGAA AATGCAAGAG GCTCCAGAGT CAGCTACAGT TATATTTGCA 
GGAGATACAA ATCTAAGGGA TCGAGAGGTT ACCAGATGTG GTGGTTTACC CAACAACATT 
GTGGATGTCT GGGAGTTTTT GGGCAAACCT AAACATTGCC AGTATACATG GGATACACAA 
ATGAACTCTA ATCTTGGAAT AACTGCTGCT TGTAAACTTC GTTTTGATCG AATATTTTTC 
AGAGCAGCAG CAGAAGAGGG ACACATTATT CCCCGAAGTT TGGACCTTCT TGGATTAGAA 
AAACTGGACT GTGGTAGATT TCCTAGTGAT CACTGGGGTC TTCTGTGCAA CTTAGATATA 
ATATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TDP2 ( NM_016614.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_057698.2 Homo sapiens tyrosyl-DNA phosphodiesterase 2 (TDP2), mRNA.

Price & Availability↑

CloneID	OHu13222
Clone ID Related Accession (Same CDS sequence)	NM_016614.3 , NM_016614.2
Accession Version	NM_016614.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	tyrosyl-DNA phosphodiesterase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC017553.2. This sequence is a reference standard in the RefSeqGene project. On Oct 7, 2002 this sequence version replaced NM_016614.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017553.2, SRR1163658.183402.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTGG GGAGTTGCCT GGAGGGCGGG AGGGAGGCGG CGGAGGAAGA GGGCGAGCCT 
GAGGTGAAAA AGCGGCGACT TCTGTGTGTG GAGTTTGCCT CGGTCGCAAG CTGCGATGCC 
GCAGTGGCTC AGTGCTTCCT GGCCGAGAAC GACTGGGAGA TGGAAAGGGC TCTGAACTCC 
TACTTCGAGC CTCCGGTGGA GGAGAGCGCC TTGGAACGCC GACCTGAAAC CATCTCTGAG 
CCCAAGACCT ATGTTGACCT AACCAATGAA GAAACAACTG ATTCCACCAC TTCTAAAATC 
AGCCCATCTG AAGATACTCA GCAAGAAAAT GGCAGCATGT TCTCTCTCAT TACCTGGAAT 
ATTGATGGAT TAGATCTAAA CAATCTGTCA GAGAGGGCTC GAGGGGTGTG TTCCTACTTA 
GCTTTGTACA GCCCAGATGT GATATTTCTA CAGGAAGTTA TTCCCCCATA TTATAGCTAC 
CTAAAGAAGA GATCAAGTAA TTATGAGATT ATTACAGGTC ATGAAGAAGG ATATTTCACA 
GCTATAATGT TGAAGAAATC AAGAGTGAAA TTAAAAAGCC AAGAGATTAT TCCTTTTCCA 
AGTACCAAAA TGATGAGAAA CCTTTTATGT GTGCATGTGA ACGTGTCAGG AAATGAGCTT 
TGCCTTATGA CATCCCATTT GGAGAGCACC AGAGGGCATG CTGCGGAACG AATGAATCAG 
TTAAAAATGG TTTTAAAGAA AATGCAAGAG GCTCCAGAGT CAGCTACAGT TATATTTGCA 
GGAGATACAA ATCTAAGGGA TCGAGAGGTT ACCAGATGTG GTGGTTTACC CAACAACATT 
GTGGATGTCT GGGAGTTTTT GGGCAAACCT AAACATTGCC AGTATACATG GGATACACAA 
ATGAACTCTA ATCTTGGAAT AACTGCTGCT TGTAAACTTC GTTTTGATCG AATATTTTTC 
AGAGCAGCAG CAGAAGAGGG ACACATTATT CCCCGAAGTT TGGACCTTCT TGGATTAGAA 
AAACTGGACT GTGGTAGATT TCCTAGTGAT CACTGGGGTC TTCTGTGCAA CTTAGATATA 
ATATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057698.2
CDS	26..1114
Translation	MELGSCLEGGREAAEEEGEPEVKKRRLLCVEFASVASCDAAVAQCFLAENDWEMERALNSYFEPPVEESALERRPETISEPKTYVDLTNEETTDSTTSKISPSEDTQQENGSMFSLITWNIDGLDLNNLSERARGVCSYLALYSPDVIFLQEVIPPYYSYLKKRSSNYEIITGHEEGYFTAIMLKKSRVKLKSQEIIPFPSTKMMRNLLCVHVNVSGNELCLMTSHLESTRGHAAERMNQLKMVLKKMQEAPESATVIFAGDTNLRDREVTRCGGLPNNIVDVWEFLGKPKHCQYTWDTQMNSNLGITAACKLRFDRIFFRAAAEEGHIIPRSLDLLGLEKLDCGRFPSDHWGLLCNLDIIL

Target ORF information:

RefSeq Version	NM_016614.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tyrosyl-DNA phosphodiesterase 2 (TDP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016614.2

ORF Insert Sequence:

ATGGAGTTGG GGAGTTGCCT GGAGGGCGGG AGGGAGGCGG CGGAGGAAGA GGGCGAGCCT 
GAGGTGAAAA AGCGGCGACT TCTGTGTGTG GAGTTTGCCT CGGTCGCAAG CTGCGATGCC 
GCAGTGGCTC AGTGCTTCCT GGCCGAGAAC GACTGGGAGA TGGAAAGGGC TCTGAACTCC 
TACTTCGAGC CTCCGGTGGA GGAGAGCGCC TTGGAACGCC GACCTGAAAC CATCTCTGAG 
CCCAAGACCT ATGTTGACCT AACCAATGAA GAAACAACTG ATTCCACCAC TTCTAAAATC 
AGCCCATCTG AAGATACTCA GCAAGAAAAT GGCAGCATGT TCTCTCTCAT TACCTGGAAT 
ATTGATGGAT TAGATCTAAA CAATCTGTCA GAGAGGGCTC GAGGGGTGTG TTCCTACTTA 
GCTTTGTACA GCCCAGATGT GATATTTCTA CAGGAAGTTA TTCCCCCATA TTATAGCTAC 
CTAAAGAAGA GATCAAGTAA TTATGAGATT ATTACAGGTC ATGAAGAAGG ATATTTCACA 
GCTATAATGT TGAAGAAATC AAGAGTGAAA TTAAAAAGCC AAGAGATTAT TCCTTTTCCA 
AGTACCAAAA TGATGAGAAA CCTTTTATGT GTGCATGTGA ACGTGTCAGG AAATGAGCTT 
TGCCTTATGA CATCCCATTT GGAGAGCACC AGAGGGCATG CTGCGGAACG AATGAATCAG 
TTAAAAATGG TTTTAAAGAA AATGCAAGAG GCTCCAGAGT CAGCTACAGT TATATTTGCA 
GGAGATACAA ATCTAAGGGA TCGAGAGGTT ACCAGATGTG GTGGTTTACC CAACAACATT 
GTGGATGTCT GGGAGTTTTT GGGCAAACCT AAACATTGCC AGTATACATG GGATACACAA 
ATGAACTCTA ATCTTGGAAT AACTGCTGCT TGTAAACTTC GTTTTGATCG AATATTTTTC 
AGAGCAGCAG CAGAAGAGGG ACACATTATT CCCCGAAGTT TGGACCTTCT TGGATTAGAA 
AAACTGGACT GTGGTAGATT TCCTAGTGAT CACTGGGGTC TTCTGTGCAA CTTAGATATA 
ATATTGTAA