SCLY cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCLY
Entrez Gene ID	51540
Full Name	selenocysteine lyase
Synonyms	SCL,hSCL
General protein information	Preferred Names selenocysteine lyase Names selenocysteine lyase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q37.3
Summary	Selenocysteine lyase (SCLY; EC 4.4.1.16) catalyzes the pyridoxal 5-prime phosphate-dependent conversion of L-selenocysteine to L-alanine and elemental selenium (Mihara et al., 2000 [PubMed 10692412]).[supplied by OMIM, Mar 2008].
Disorder MIM:	611056

mRNA and Protein(s)

mRNA	Protein	Name
NM_016510.5	NP_057594.4	selenocysteine lyase
NM_016510.6	NP_057594.5	selenocysteine lyase
NM_016510.7	NP_057594.5	selenocysteine lyase

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SCLY	NP_001077273.1
Rattus norvegicus (Norway rat)	Scly	NP_001007756.1
Xenopus tropicalis (tropical clawed frog)	scly	NP_001011164.1
Homo sapiens (human)	SCLY	NP_057594.4
Canis lupus familiaris (dog)	SCLY	XP_005635980.1
Caenorhabditis elegans (roundworm)	F13H8.9	NP_495258.1
	SCLY	XP_001086235.2
Mus musculus (house mouse)	Scly	NP_057926.2
Pan troglodytes (chimpanzee)	SCLY	XP_516185.3
Gallus gallus (chicken)	SCLY	NP_001258454.1
Danio rerio (zebrafish)	scly	NP_001103853.1

Gene Ontology
Bibliography

Related articles in PubMed

Biochemical discrimination between selenium and sulfur 1: a single residue provides selenium specificity to human selenocysteine lyase.
Collins R, Johansson AL, Karlberg T, Markova N, van den Berg S, Olesen K, Hammarstr?m M, Flores A, Sch?ler H, Schiavone LH, Brzezinski P, Arn?r ES, H?gbom M
PloS one7(1)e30581(2012)

Biochemical discrimination between selenium and sulfur 2: mechanistic investigation of the selenium specificity of human selenocysteine lyase.
Johansson AL, Collins R, Arn?r ES, Brzezinski P, H?gbom M
PloS one7(1)e30528(2012)

Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells.
Lee OH, Kim H, He Q, Baek HJ, Yang D, Chen LY, Liang J, Chae HK, Safari A, Liu D, Songyang Z
Molecular & cellular proteomics : MCP10(2)M110.001628(2011 Feb)

[Differential display of messenger RNA and identification of selenocysteine lyase gene in hepatocellular carcinoma cells transiently expressing hepatitis C virus core protein].
Yepes JO, Luz Gunturiz M, Henao LF, Navas MC, Balc?zar N, G?mez LA
Biomedica : revista del Instituto Nacional de Salud26(2)194-205(2006 Jun)

cDNA cloning, purification, and characterization of mouse liver selenocysteine lyase. Candidate for selenium delivery protein in selenoprotein synthesis.
Mihara H, Kurihara T, Watanabe T, Yoshimura T, Esaki N
The Journal of biological chemistry275(9)6195-200(2000 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the X-ray crystal structure of human selenocysteine lyase and the key residue that provides the selenocysteine specificity
Title: Biochemical discrimination between selenium and sulfur 1: a single residue provides selenium specificity to human selenocysteine lyase.

A reaction mechanism whereby the Selenocysteine over Cysteine specificity is achieved using a combination of chemical and physico-mechanical control mechanisms.
Title: Biochemical discrimination between selenium and sulfur 2: mechanistic investigation of the selenium specificity of human selenocysteine lyase.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Results confirm that HCV core protein is associated with specific changes in mRNA expression, including the gene for selenocysteine lyase, which may be involved in the pathophysiology of hepatocellular carcinoma.
Title: [Differential display of messenger RNA and identification of selenocysteine lyase gene in hepatocellular carcinoma cells transiently expressing hepatitis C virus core protein].

PMID:10692412 concerns mostly mouse selenocysteine lyase, however, refers to the sequence of the N-terminal region of putative human protein.
Title: cDNA cloning, purification, and characterization of mouse liver selenocysteine lyase. Candidate for selenium delivery protein in selenoprotein synthesis.

The following SCLY gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCLY cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01421	NM_016510.5 Latest version!	Homo sapiens selenocysteine lyase (SCLY), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu107070	NM_016510.7 Latest version!	Homo sapiens selenocysteine lyase (SCLY), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu107070	NM_016510.6	Homo sapiens selenocysteine lyase (SCLY), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCLY ( NM_016510.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057594.4 Homo sapiens selenocysteine lyase (SCLY), mRNA.

Price & Availability↑

CloneID	OHu01421
Clone ID Related Accession (Same CDS sequence)	NM_016510.5
Accession Version	NM_016510.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	selenocysteine lyase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB209458.2, BC007891.2, AK293283.1, AC016757.10 and BC018966.2. On Feb 18, 2011 this sequence version replaced NM_016510.4. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.520968.1, SRR1803611.254025.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCGGCA GCAGTGGGGC GGGGATGGAG GCGGCCGTGG CGCCGGGGAG GGATGCGCCG 
GCACCCGCGG CGAGTCAGCC CAGCGGCTGC GGGAAACACA ACTCGCCGGA GAGGAAAGTT 
TATATGGACT ATAATGCAAC GACTCCCCTG GAGCCAGAAG TTATCCAGGC CATGACCAAG 
GCCATGTGGG AAGCCTGGGG AAATCCCAGC AGCCCGTATT CAGCAGGAAG AAAGGCCAAG 
GATATTATAA ATGCAGCTCG GGAAAGCCTC GCGAAGATGA TAGGGGGGAA ACCTCAAGAT 
ATAATCTTCA CTTCCGGGGG CACTGAGTCA AATAATTTAG TAATCCATTC TGTGGTGAAA 
CATTTCCACG CAAACCAGAC CTCAAAGGGA CACACAGGTG GGCACCACAG CCCAGTGAAG 
GGGGCCAAGC CCCATTTCAT TACTTCCTCG GTGGAACACG ACTCCATCCG GCTGCCCCTG 
GAGCACCTGG TGGAAGAACA AGTGGCAGCG GTCACCTTTG TCCCGGTGTC CAAGGTGAGC 
GGGCAGGCAG AGGTGGACGA CATCCTCGCG GCAGTCCGCC CGACCACACG CCTCGTGACC 
ATCATGCTGG CCAACAATGA GACTGGCATT GTCATGCCTG TCCCTGAAAT CAGTCAGCGC 
ATTAAAGCCC TGAACCAGGA ACGGGTGGCA GCTGGGCTAC CTCCCATCCT CGTGCACACG 
GATGCTGCAC AGGCCTTGGG GAAGCAGCGC GTGGATGTGG AGGACCTGGG CGTGGACTTC 
CTTACAATCG TGGGGCACAA GTTTTATGGT CCCAGGATTG GCGCACTTTA TATACGAGGA 
CTTGGTGAAT TTACCCCTCT CTACCCTATG CTATTTGGAG GTGGACAAGA ACGGAATTTC 
AGGCCAGGGA CAGAGAACAC CCCAATGATT GCTGGCCTTG GGAAGGCCGC GGAGCTGGTG 
ACCCAGAACT GCGAGGCTTA TGAGGCCCAC ATGAGGGACG TCCGCGACTA CCTGGAAGAG 
AGGCTGGAAG CTGAATTCGG TCAGAAGAGA ATCCATCTGA ATAGCCAGTT TCCAGGCACC 
CAGCGGCTTC CCAATACCTG TAACTTTTCC ATCCGGGGAC CCCGGCTTCA AGGCCACGTG 
GTGCTTGCGC AGTGCCGAGT GCTGATGGCC AGTGTGGGGG CCGCGTGCCA CTCGGACCAC 
GGGGACCAGC CGTCCCCAGT GCTGCTGAGC TACGGTGTCC CCTTCGACGT GGCCAGGAAC 
GCGCTCCGGC TCAGCGTGGG CCGCAGCACC ACCAGGGCCG AGGTGGACCT CGTCGTGCAG 
GACCTGAAGC AGGCCGTGGC GCAGCTGGAG GACCAGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057594.4
CDS	108..1469
Translation	MPGSSGAGMEAAVAPGRDAPAPAASQPSGCGKHNSPERKVYMDYNATTPLEPEVIQAMTKAMWEAWGNPSSPYSAGRKAKDIINAARESLAKMIGGKPQDIIFTSGGTESNNLVIHSVVKHFHANQTSKGHTGGHHSPVKGAKPHFITSSVEHDSIRLPLEHLVEEQVAAVTFVPVSKVSGQAEVDDILAAVRPTTRLVTIMLANNETGIVMPVPEISQRIKALNQERVAAGLPPILVHTDAAQALGKQRVDVEDLGVDFLTIVGHKFYGPRIGALYIRGLGEFTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAAELVTQNCEAYEAHMRDVRDYLEERLEAEFGQKRIHLNSQFPGTQRLPNTCNFSIRGPRLQGHVVLAQCRVLMASVGAACHSDHGDQPSPVLLSYGVPFDVARNALRLSVGRSTTRAEVDLVVQDLKQAVAQLEDQA

Target ORF information:

RefSeq Version	NM_016510.5
Organism	Homo sapiens(human)
Definition	Homo sapiens selenocysteine lyase (SCLY), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016510.5

ORF Insert Sequence:

ATGCCCGGCA GCAGTGGGGC GGGGATGGAG GCGGCCGTGG CGCCGGGGAG GGATGCGCCG 
GCACCCGCGG CGAGTCAGCC CAGCGGCTGC GGGAAACACA ACTCGCCGGA GAGGAAAGTT 
TATATGGACT ATAATGCAAC GACTCCCCTG GAGCCAGAAG TTATCCAGGC CATGACCAAG 
GCCATGTGGG AAGCCTGGGG AAATCCCAGC AGCCCGTATT CAGCAGGAAG AAAGGCCAAG 
GATATTATAA ATGCAGCTCG GGAAAGCCTC GCGAAGATGA TAGGGGGGAA ACCTCAAGAT 
ATAATCTTCA CTTCCGGGGG CACTGAGTCA AATAATTTAG TAATCCATTC TGTGGTGAAA 
CATTTCCACG CAAACCAGAC CTCAAAGGGA CACACAGGTG GGCACCACAG CCCAGTGAAG 
GGGGCCAAGC CCCATTTCAT TACTTCCTCG GTGGAACACG ACTCCATCCG GCTGCCCCTG 
GAGCACCTGG TGGAAGAACA AGTGGCAGCG GTCACCTTTG TCCCGGTGTC CAAGGTGAGC 
GGGCAGGCAG AGGTGGACGA CATCCTCGCG GCAGTCCGCC CGACCACACG CCTCGTGACC 
ATCATGCTGG CCAACAATGA GACTGGCATT GTCATGCCTG TCCCTGAAAT CAGTCAGCGC 
ATTAAAGCCC TGAACCAGGA ACGGGTGGCA GCTGGGCTAC CTCCCATCCT CGTGCACACG 
GATGCTGCAC AGGCCTTGGG GAAGCAGCGC GTGGATGTGG AGGACCTGGG CGTGGACTTC 
CTTACAATCG TGGGGCACAA GTTTTATGGT CCCAGGATTG GCGCACTTTA TATACGAGGA 
CTTGGTGAAT TTACCCCTCT CTACCCTATG CTATTTGGAG GTGGACAAGA ACGGAATTTC 
AGGCCAGGGA CAGAGAACAC CCCAATGATT GCTGGCCTTG GGAAGGCCGC GGAGCTGGTG 
ACCCAGAACT GCGAGGCTTA TGAGGCCCAC ATGAGGGACG TCCGCGACTA CCTGGAAGAG 
AGGCTGGAAG CTGAATTCGG TCAGAAGAGA ATCCATCTGA ATAGCCAGTT TCCAGGCACC 
CAGCGGCTTC CCAATACCTG TAACTTTTCC ATCCGGGGAC CCCGGCTTCA AGGCCACGTG 
GTGCTTGCGC AGTGCCGAGT GCTGATGGCC AGTGTGGGGG CCGCGTGCCA CTCGGACCAC 
GGGGACCAGC CGTCCCCAGT GCTGCTGAGC TACGGTGTCC CCTTCGACGT GGCCAGGAAC 
GCGCTCCGGC TCAGCGTGGG CCGCAGCACC ACCAGGGCCG AGGTGGACCT CGTCGTGCAG 
GACCTGAAGC AGGCCGTGGC GCAGCTGGAG GACCAGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCLY ( NM_016510.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_057594.5 Homo sapiens selenocysteine lyase (SCLY), mRNA.

Price & Availability↑

CloneID	OHu107070
Clone ID Related Accession (Same CDS sequence)	NM_016510.6 , NM_016510.7
Accession Version	NM_016510.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	selenocysteine lyase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB209458.2, BC007891.2, AK293283.1, AC016757.10 and BC018966.2. On Jul 4, 2018 this sequence version replaced NM_016510.5. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1050589.1, SRR1163655.520968.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: lack of evidence for use of upstream AUG ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGG CCGTGGCGCC GGGGAGGGAT GCGCCGGCAC CCGCGGCGAG TCAGCCCAGC 
GGCTGCGGGA AACACAACTC GCCGGAGAGG AAAGTTTATA TGGACTATAA TGCAACGACT 
CCCCTGGAGC CAGAAGTTAT CCAGGCCATG ACCAAGGCCA TGTGGGAAGC CTGGGGAAAT 
CCCAGCAGCC CGTATTCAGC AGGAAGAAAG GCCAAGGATA TTATAAATGC AGCTCGGGAA 
AGCCTCGCGA AGATGATAGG GGGGAAACCT CAAGATATAA TCTTCACTTC CGGGGGCACT 
GAGTCAAATA ATTTAGTAAT CCATTCTGTG GTGAAACATT TCCACGCAAA CCAGACCTCA 
AAGGGACACA CAGGTGGGCA CCACAGCCCA GTGAAGGGGG CCAAGCCCCA TTTCATTACT 
TCCTCGGTGG AACACGACTC CATCCGGCTG CCCCTGGAGC ACCTGGTGGA AGAACAAGTG 
GCAGCGGTCA CCTTTGTCCC GGTGTCCAAG GTGAGCGGGC AGGCAGAGGT GGACGACATC 
CTCGCGGCAG TCCGCCCGAC CACACGCCTC GTGACCATCA TGCTGGCCAA CAATGAGACT 
GGCATTGTCA TGCCTGTCCC TGAAATCAGT CAGCGCATTA AAGCCCTGAA CCAGGAACGG 
GTGGCAGCTG GGCTACCTCC CATCCTCGTG CACACGGATG CTGCACAGGC CTTGGGGAAG 
CAGCGCGTGG ATGTGGAGGA CCTGGGCGTG GACTTCCTTA CAATCGTGGG GCACAAGTTT 
TATGGTCCCA GGATTGGCGC ACTTTATATA CGAGGACTTG GTGAATTTAC CCCTCTCTAC 
CCTATGCTAT TTGGAGGTGG ACAAGAACGG AATTTCAGGC CAGGGACAGA GAACACCCCA 
ATGATTGCTG GCCTTGGGAA GGCCGCGGAG CTGGTGACCC AGAACTGCGA GGCTTATGAG 
GCCCACATGA GGGACGTCCG CGACTACCTG GAAGAGAGGC TGGAAGCTGA ATTCGGTCAG 
AAGAGAATCC ATCTGAATAG CCAGTTTCCA GGCACCCAGC GGCTTCCCAA TACCTGTAAC 
TTTTCCATCC GGGGACCCCG GCTTCAAGGC CACGTGGTGC TTGCGCAGTG CCGAGTGCTG 
ATGGCCAGTG TGGGGGCCGC GTGCCACTCG GACCACGGGG ACCAGCCGTC CCCAGTGCTG 
CTGAGCTACG GTGTCCCCTT CGACGTGGCC AGGAACGCGC TCCGGCTCAG CGTGGGCCGC 
AGCACCACCA GGGCCGAGGT GGACCTCGTC GTGCAGGACC TGAAGCAGGC CGTGGCGCAG 
CTGGAGGACC AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057594.5
CDS	55..1392
Translation	MEAAVAPGRDAPAPAASQPSGCGKHNSPERKVYMDYNATTPLEPEVIQAMTKAMWEAWGNPSSPYSAGRKAKDIINAARESLAKMIGGKPQDIIFTSGGTESNNLVIHSVVKHFHANQTSKGHTGGHHSPVKGAKPHFITSSVEHDSIRLPLEHLVEEQVAAVTFVPVSKVSGQAEVDDILAAVRPTTRLVTIMLANNETGIVMPVPEISQRIKALNQERVAAGLPPILVHTDAAQALGKQRVDVEDLGVDFLTIVGHKFYGPRIGALYIRGLGEFTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAAELVTQNCEAYEAHMRDVRDYLEERLEAEFGQKRIHLNSQFPGTQRLPNTCNFSIRGPRLQGHVVLAQCRVLMASVGAACHSDHGDQPSPVLLSYGVPFDVARNALRLSVGRSTTRAEVDLVVQDLKQAVAQLEDQA

Target ORF information:

RefSeq Version	NM_016510.6
Organism	Homo sapiens(human)
Definition	Homo sapiens selenocysteine lyase (SCLY), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016510.6

ORF Insert Sequence:

ATGGAGGCGG CCGTGGCGCC GGGGAGGGAT GCGCCGGCAC CCGCGGCGAG TCAGCCCAGC 
GGCTGCGGGA AACACAACTC GCCGGAGAGG AAAGTTTATA TGGACTATAA TGCAACGACT 
CCCCTGGAGC CAGAAGTTAT CCAGGCCATG ACCAAGGCCA TGTGGGAAGC CTGGGGAAAT 
CCCAGCAGCC CGTATTCAGC AGGAAGAAAG GCCAAGGATA TTATAAATGC AGCTCGGGAA 
AGCCTCGCGA AGATGATAGG GGGGAAACCT CAAGATATAA TCTTCACTTC CGGGGGCACT 
GAGTCAAATA ATTTAGTAAT CCATTCTGTG GTGAAACATT TCCACGCAAA CCAGACCTCA 
AAGGGACACA CAGGTGGGCA CCACAGCCCA GTGAAGGGGG CCAAGCCCCA TTTCATTACT 
TCCTCGGTGG AACACGACTC CATCCGGCTG CCCCTGGAGC ACCTGGTGGA AGAACAAGTG 
GCAGCGGTCA CCTTTGTCCC GGTGTCCAAG GTGAGCGGGC AGGCAGAGGT GGACGACATC 
CTCGCGGCAG TCCGCCCGAC CACACGCCTC GTGACCATCA TGCTGGCCAA CAATGAGACT 
GGCATTGTCA TGCCTGTCCC TGAAATCAGT CAGCGCATTA AAGCCCTGAA CCAGGAACGG 
GTGGCAGCTG GGCTACCTCC CATCCTCGTG CACACGGATG CTGCACAGGC CTTGGGGAAG 
CAGCGCGTGG ATGTGGAGGA CCTGGGCGTG GACTTCCTTA CAATCGTGGG GCACAAGTTT 
TATGGTCCCA GGATTGGCGC ACTTTATATA CGAGGACTTG GTGAATTTAC CCCTCTCTAC 
CCTATGCTAT TTGGAGGTGG ACAAGAACGG AATTTCAGGC CAGGGACAGA GAACACCCCA 
ATGATTGCTG GCCTTGGGAA GGCCGCGGAG CTGGTGACCC AGAACTGCGA GGCTTATGAG 
GCCCACATGA GGGACGTCCG CGACTACCTG GAAGAGAGGC TGGAAGCTGA ATTCGGTCAG 
AAGAGAATCC ATCTGAATAG CCAGTTTCCA GGCACCCAGC GGCTTCCCAA TACCTGTAAC 
TTTTCCATCC GGGGACCCCG GCTTCAAGGC CACGTGGTGC TTGCGCAGTG CCGAGTGCTG 
ATGGCCAGTG TGGGGGCCGC GTGCCACTCG GACCACGGGG ACCAGCCGTC CCCAGTGCTG 
CTGAGCTACG GTGTCCCCTT CGACGTGGCC AGGAACGCGC TCCGGCTCAG CGTGGGCCGC 
AGCACCACCA GGGCCGAGGT GGACCTCGTC GTGCAGGACC TGAAGCAGGC CGTGGCGCAG 
CTGGAGGACC AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCLY ( NM_016510.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_057594.5 Homo sapiens selenocysteine lyase (SCLY), mRNA.

Price & Availability↑

CloneID	OHu107070
Clone ID Related Accession (Same CDS sequence)	NM_016510.6 , NM_016510.7
Accession Version	NM_016510.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	selenocysteine lyase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB209458.2, BC007891.2, AK293283.1, AC016757.10 and BC018966.2. On May 17, 2019 this sequence version replaced NM_016510.6. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1050589.1, SRR1163655.520968.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: lack of evidence for use of upstream AUG MANE Ensembl match :: ENST00000254663.12/ ENSP00000254663.7 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGG CCGTGGCGCC GGGGAGGGAT GCGCCGGCAC CCGCGGCGAG TCAGCCCAGC 
GGCTGCGGGA AACACAACTC GCCGGAGAGG AAAGTTTATA TGGACTATAA TGCAACGACT 
CCCCTGGAGC CAGAAGTTAT CCAGGCCATG ACCAAGGCCA TGTGGGAAGC CTGGGGAAAT 
CCCAGCAGCC CGTATTCAGC AGGAAGAAAG GCCAAGGATA TTATAAATGC AGCTCGGGAA 
AGCCTCGCGA AGATGATAGG GGGGAAACCT CAAGATATAA TCTTCACTTC CGGGGGCACT 
GAGTCAAATA ATTTAGTAAT CCATTCTGTG GTGAAACATT TCCACGCAAA CCAGACCTCA 
AAGGGACACA CAGGTGGGCA CCACAGCCCA GTGAAGGGGG CCAAGCCCCA TTTCATTACT 
TCCTCGGTGG AACACGACTC CATCCGGCTG CCCCTGGAGC ACCTGGTGGA AGAACAAGTG 
GCAGCGGTCA CCTTTGTCCC GGTGTCCAAG GTGAGCGGGC AGGCAGAGGT GGACGACATC 
CTCGCGGCAG TCCGCCCGAC CACACGCCTC GTGACCATCA TGCTGGCCAA CAATGAGACT 
GGCATTGTCA TGCCTGTCCC TGAAATCAGT CAGCGCATTA AAGCCCTGAA CCAGGAACGG 
GTGGCAGCTG GGCTACCTCC CATCCTCGTG CACACGGATG CTGCACAGGC CTTGGGGAAG 
CAGCGCGTGG ATGTGGAGGA CCTGGGCGTG GACTTCCTTA CAATCGTGGG GCACAAGTTT 
TATGGTCCCA GGATTGGCGC ACTTTATATA CGAGGACTTG GTGAATTTAC CCCTCTCTAC 
CCTATGCTAT TTGGAGGTGG ACAAGAACGG AATTTCAGGC CAGGGACAGA GAACACCCCA 
ATGATTGCTG GCCTTGGGAA GGCCGCGGAG CTGGTGACCC AGAACTGCGA GGCTTATGAG 
GCCCACATGA GGGACGTCCG CGACTACCTG GAAGAGAGGC TGGAAGCTGA ATTCGGTCAG 
AAGAGAATCC ATCTGAATAG CCAGTTTCCA GGCACCCAGC GGCTTCCCAA TACCTGTAAC 
TTTTCCATCC GGGGACCCCG GCTTCAAGGC CACGTGGTGC TTGCGCAGTG CCGAGTGCTG 
ATGGCCAGTG TGGGGGCCGC GTGCCACTCG GACCACGGGG ACCAGCCGTC CCCAGTGCTG 
CTGAGCTACG GTGTCCCCTT CGACGTGGCC AGGAACGCGC TCCGGCTCAG CGTGGGCCGC 
AGCACCACCA GGGCCGAGGT GGACCTCGTC GTGCAGGACC TGAAGCAGGC CGTGGCGCAG 
CTGGAGGACC AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057594.5
CDS	55..1392
Translation	MEAAVAPGRDAPAPAASQPSGCGKHNSPERKVYMDYNATTPLEPEVIQAMTKAMWEAWGNPSSPYSAGRKAKDIINAARESLAKMIGGKPQDIIFTSGGTESNNLVIHSVVKHFHANQTSKGHTGGHHSPVKGAKPHFITSSVEHDSIRLPLEHLVEEQVAAVTFVPVSKVSGQAEVDDILAAVRPTTRLVTIMLANNETGIVMPVPEISQRIKALNQERVAAGLPPILVHTDAAQALGKQRVDVEDLGVDFLTIVGHKFYGPRIGALYIRGLGEFTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAAELVTQNCEAYEAHMRDVRDYLEERLEAEFGQKRIHLNSQFPGTQRLPNTCNFSIRGPRLQGHVVLAQCRVLMASVGAACHSDHGDQPSPVLLSYGVPFDVARNALRLSVGRSTTRAEVDLVVQDLKQAVAQLEDQA

Target ORF information:

RefSeq Version	NM_016510.7
Organism	Homo sapiens(human)
Definition	Homo sapiens selenocysteine lyase (SCLY), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016510.7

ORF Insert Sequence:

ATGGAGGCGG CCGTGGCGCC GGGGAGGGAT GCGCCGGCAC CCGCGGCGAG TCAGCCCAGC 
GGCTGCGGGA AACACAACTC GCCGGAGAGG AAAGTTTATA TGGACTATAA TGCAACGACT 
CCCCTGGAGC CAGAAGTTAT CCAGGCCATG ACCAAGGCCA TGTGGGAAGC CTGGGGAAAT 
CCCAGCAGCC CGTATTCAGC AGGAAGAAAG GCCAAGGATA TTATAAATGC AGCTCGGGAA 
AGCCTCGCGA AGATGATAGG GGGGAAACCT CAAGATATAA TCTTCACTTC CGGGGGCACT 
GAGTCAAATA ATTTAGTAAT CCATTCTGTG GTGAAACATT TCCACGCAAA CCAGACCTCA 
AAGGGACACA CAGGTGGGCA CCACAGCCCA GTGAAGGGGG CCAAGCCCCA TTTCATTACT 
TCCTCGGTGG AACACGACTC CATCCGGCTG CCCCTGGAGC ACCTGGTGGA AGAACAAGTG 
GCAGCGGTCA CCTTTGTCCC GGTGTCCAAG GTGAGCGGGC AGGCAGAGGT GGACGACATC 
CTCGCGGCAG TCCGCCCGAC CACACGCCTC GTGACCATCA TGCTGGCCAA CAATGAGACT 
GGCATTGTCA TGCCTGTCCC TGAAATCAGT CAGCGCATTA AAGCCCTGAA CCAGGAACGG 
GTGGCAGCTG GGCTACCTCC CATCCTCGTG CACACGGATG CTGCACAGGC CTTGGGGAAG 
CAGCGCGTGG ATGTGGAGGA CCTGGGCGTG GACTTCCTTA CAATCGTGGG GCACAAGTTT 
TATGGTCCCA GGATTGGCGC ACTTTATATA CGAGGACTTG GTGAATTTAC CCCTCTCTAC 
CCTATGCTAT TTGGAGGTGG ACAAGAACGG AATTTCAGGC CAGGGACAGA GAACACCCCA 
ATGATTGCTG GCCTTGGGAA GGCCGCGGAG CTGGTGACCC AGAACTGCGA GGCTTATGAG 
GCCCACATGA GGGACGTCCG CGACTACCTG GAAGAGAGGC TGGAAGCTGA ATTCGGTCAG 
AAGAGAATCC ATCTGAATAG CCAGTTTCCA GGCACCCAGC GGCTTCCCAA TACCTGTAAC 
TTTTCCATCC GGGGACCCCG GCTTCAAGGC CACGTGGTGC TTGCGCAGTG CCGAGTGCTG 
ATGGCCAGTG TGGGGGCCGC GTGCCACTCG GACCACGGGG ACCAGCCGTC CCCAGTGCTG 
CTGAGCTACG GTGTCCCCTT CGACGTGGCC AGGAACGCGC TCCGGCTCAG CGTGGGCCGC 
AGCACCACCA GGGCCGAGGT GGACCTCGTC GTGCAGGACC TGAAGCAGGC CGTGGCGCAG 
CTGGAGGACC AGGCCTAG