DDX47 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX47
Entrez Gene ID	51202
Full Name	DEAD-box helicase 47
Synonyms	E4-DBP,HQ0256,MSTP162,RRP3
General protein information	Preferred Names DEAD-box helicase 47 Names probable ATP-dependent RNA helicase DDX47 DEAD (Asp-Glu-Ala-Asp) box polypeptide 47 DEAD box polypeptide 47 DEAD box protein 47 E4-DEAD box protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.1
Summary	This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	615428

mRNA and Protein(s)

mRNA	Protein	Name
NM_201224.1	NP_957518.1	probable ATP-dependent RNA helicase DDX47 isoform 2
NM_201224.2	NP_957518.1	probable ATP-dependent RNA helicase DDX47 isoform 2
NM_016355.3	NP_057439.2	probable ATP-dependent RNA helicase DDX47 isoform 1
NM_016355.4	NP_057439.2	probable ATP-dependent RNA helicase DDX47 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDX47	NP_057439.2
Pan troglodytes (chimpanzee)	DDX47	NP_001233477.1
	DDX47	XP_001086352.1
Bos taurus (cattle)	DDX47	NP_001039850.1
Gallus gallus (chicken)	DDX47	NP_001007854.1
Danio rerio (zebrafish)	ddx47	NP_001018522.1
Saccharomyces cerevisiae (baker's yeast)	RRP3	NP_011932.2
Mus musculus (house mouse)	Ddx47	NP_080636.2
Drosophila melanogaster (fruit fly)	CG9253	NP_610090.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009808	XP_318913.4
Caenorhabditis elegans (roundworm)	CELE_T26G10.1	NP_499069.1
Arabidopsis thaliana (thale cress)	AT5G60990	NP_568931.1
Canis lupus familiaris (dog)	DDX47	XP_534885.2
Eremothecium gossypii	AGOS_AAL041C	NP_982501.2
Schizosaccharomyces pombe (fission yeast)	SPAC823.08c	NP_593835.1
Neurospora crassa	NCU04504	XP_956915.1
Oryza sativa (rice)	Os03g0669000	NP_001050861.1
Xenopus tropicalis (tropical clawed frog)	ddx47	XP_002934775.1
Rattus norvegicus (Norway rat)	Ddx47	NP_001015005.2
Kluyveromyces lactis	KLLA0C14608g	XP_452854.1
Magnaporthe oryzae (rice blast fungus)	MGG_17539	XP_003719049.1

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Protein microarray analysis identifies human cellular prion protein interactors.
Satoh J, Obayashi S, Misawa T, Sumiyoshi K, Oosumi K, Tabunoki H
Neuropathology and applied neurobiology35(1)16-35(2009 Feb)

Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.
H?ck J, Weinmann L, Ender C, R?del S, Kremmer E, Raabe M, Urlaub H, Meister G
EMBO reports8(11)1052-60(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GABARAP and DDX 47 are involved in the apoptotic process
Title: GABAA receptor-associated protein (GABARAP) induces apoptosis by interacting with DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47).

The following DDX47 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX47 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29268	NM_016355.4 Latest version!	Homo sapiens DEAD-box helicase 47 (DDX47), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu02834	NM_201224.2 Latest version!	Homo sapiens DEAD-box helicase 47 (DDX47), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu02834	NM_201224.1	Homo sapiens DEAD-box helicase 47 (DDX47), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu29268	NM_016355.3	Homo sapiens DEAD-box helicase 47 (DDX47), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX47 ( NM_016355.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057439.2 Homo sapiens DEAD-box helicase 47 (DDX47), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29268
Clone ID Related Accession (Same CDS sequence)	NM_016355.3 , NM_016355.4
Accession Version	NM_016355.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX47 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136666.1 and BC009379.2. On Jan 26, 2004 this sequence version replaced NM_016355.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL136666.1, SRR1163655.104931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAC CCGAGGAACA CGATTCTCCG ACCGAAGCGT CCCAGCCGAT TGTGGAAGAG 
GAGGAAACTA AAACATTTAA AGACCTGGGT GTGACAGATG TGTTGTGTGA AGCTTGTGAC 
CAGTTGGGAT GGACAAAACC CACCAAGATC CAGATTGAAG CTATTCCTTT GGCCTTACAA 
GGTCGTGATA TCATTGGGCT TGCAGAAACT GGCTCTGGAA AGACAGGCGC CTTTGCTTTG 
CCCATTCTAA ACGCACTGCT GGAGACCCCG CAGCGTTTGT TTGCCCTAGT TCTTACCCCG 
ACTCGGGAGC TGGCCTTTCA GATCTCAGAG CAGTTTGAAG CCCTGGGGTC CTCTATTGGA 
GTGCAGAGTG CTGTGATTGT AGGTGGAATT GATTCAATGT CTCAATCTTT GGCCCTTGCA 
AAAAAACCAC ATATAATAAT AGCAACTCCT GGTCGACTGA TTGACCACTT GGAAAATACG 
AAAGGTTTCA ACTTGAGAGC TCTCAAATAC TTGGTCATGG ATGAAGCCGA CCGAATACTG 
AATATGGATT TTGAGACAGA GGTTGACAAG ATCCTCAAAG TGATTCCTCG AGATCGGAAA 
ACATTCCTCT TCTCTGCCAC CATGACCAAG AAGGTTCAAA AACTTCAGCG AGCAGCTCTG 
AAGAATCCTG TGAAATGTGC CGTTTCCTCT AAATACCAGA CAGTTGAAAA ATTACAGCAA 
TATTATATTT TTATTCCCTC TAAATTCAAG GATACCTACC TGGTTTATAT TCTAAATGAA 
TTGGCTGGAA ACTCCTTTAT GATATTCTGC AGCACCTGTA ATAATACCCA GAGAACAGCT 
TTGCTACTGC GAAATCTTGG CTTCACTGCC ATCCCCCTCC ATGGACAAAT GAGTCAGAGT 
AAGCGCCTAG GATCCCTTAA TAAGTTTAAG GCCAAGGCCC GTTCCATTCT TCTAGCAACT 
GACGTTGCCA GCCGAGGTTT GGACATACCT CATGTAGATG TGGTTGTCAA CTTTGACATT 
CCTACCCATT CCAAGGATTA CATCCATCGA GTAGGTCGAA CAGCTAGAGC TGGGCGCTCC 
GGAAAGGCTA TTACTTTTGT CACACAGTAT GATGTGGAAC TCTTCCAGCG CATAGAACAC 
TTAATTGGGA AGAAACTACC AGGTTTTCCA ACACAGGATG ATGAGGTTAT GATGCTGACA 
GAACGCGTCG CTGAAGCCCA AAGGTTTGCC CGAATGGAGT TAAGGGAGCA TGGAGAAAAG 
AAGAAACGCT CGCGAGAGGA TGCTGGAGAT AATGATGACA CAGAGGGTGC TATTGGTGTC 
AGGAACAAGG TGGCTGGAGG AAAAATGAAG AAGCGGAAAG GCCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057439.2
CDS	23..1390
Translation	MAAPEEHDSPTEASQPIVEEEETKTFKDLGVTDVLCEACDQLGWTKPTKIQIEAIPLALQGRDIIGLAETGSGKTGAFALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIGVQSAVIVGGIDSMSQSLALAKKPHIIIATPGRLIDHLENTKGFNLRALKYLVMDEADRILNMDFETEVDKILKVIPRDRKTFLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKDTYLVYILNELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQSKRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRSGKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEKKKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR

Target ORF information:

RefSeq Version	NM_016355.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 47 (DDX47), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016355.3

ORF Insert Sequence:

ATGGCGGCAC CCGAGGAACA CGATTCTCCG ACCGAAGCGT CCCAGCCGAT TGTGGAAGAG 
GAGGAAACTA AAACATTTAA AGACCTGGGT GTGACAGATG TGTTGTGTGA AGCTTGTGAC 
CAGTTGGGAT GGACAAAACC CACCAAGATC CAGATTGAAG CTATTCCTTT GGCCTTACAA 
GGTCGTGATA TCATTGGGCT TGCAGAAACT GGCTCTGGAA AGACAGGCGC CTTTGCTTTG 
CCCATTCTAA ACGCACTGCT GGAGACCCCG CAGCGTTTGT TTGCCCTAGT TCTTACCCCG 
ACTCGGGAGC TGGCCTTTCA GATCTCAGAG CAGTTTGAAG CCCTGGGGTC CTCTATTGGA 
GTGCAGAGTG CTGTGATTGT AGGTGGAATT GATTCAATGT CTCAATCTTT GGCCCTTGCA 
AAAAAACCAC ATATAATAAT AGCAACTCCT GGTCGACTGA TTGACCACTT GGAAAATACG 
AAAGGTTTCA ACTTGAGAGC TCTCAAATAC TTGGTCATGG ATGAAGCCGA CCGAATACTG 
AATATGGATT TTGAGACAGA GGTTGACAAG ATCCTCAAAG TGATTCCTCG AGATCGGAAA 
ACATTCCTCT TCTCTGCCAC CATGACCAAG AAGGTTCAAA AACTTCAGCG AGCAGCTCTG 
AAGAATCCTG TGAAATGTGC CGTTTCCTCT AAATACCAGA CAGTTGAAAA ATTACAGCAA 
TATTATATTT TTATTCCCTC TAAATTCAAG GATACCTACC TGGTTTATAT TCTAAATGAA 
TTGGCTGGAA ACTCCTTTAT GATATTCTGC AGCACCTGTA ATAATACCCA GAGAACAGCT 
TTGCTACTGC GAAATCTTGG CTTCACTGCC ATCCCCCTCC ATGGACAAAT GAGTCAGAGT 
AAGCGCCTAG GATCCCTTAA TAAGTTTAAG GCCAAGGCCC GTTCCATTCT TCTAGCAACT 
GACGTTGCCA GCCGAGGTTT GGACATACCT CATGTAGATG TGGTTGTCAA CTTTGACATT 
CCTACCCATT CCAAGGATTA CATCCATCGA GTAGGTCGAA CAGCTAGAGC TGGGCGCTCC 
GGAAAGGCTA TTACTTTTGT CACACAGTAT GATGTGGAAC TCTTCCAGCG CATAGAACAC 
TTAATTGGGA AGAAACTACC AGGTTTTCCA ACACAGGATG ATGAGGTTAT GATGCTGACA 
GAACGCGTCG CTGAAGCCCA AAGGTTTGCC CGAATGGAGT TAAGGGAGCA TGGAGAAAAG 
AAGAAACGCT CGCGAGAGGA TGCTGGAGAT AATGATGACA CAGAGGGTGC TATTGGTGTC 
AGGAACAAGG TGGCTGGAGG AAAAATGAAG AAGCGGAAAG GCCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX47 ( NM_016355.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057439.2 Homo sapiens DEAD-box helicase 47 (DDX47), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu29268
Clone ID Related Accession (Same CDS sequence)	NM_016355.3 , NM_016355.4
Accession Version	NM_016355.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX47 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136666.1 and BC009379.2. On Nov 22, 2018 this sequence version replaced NM_016355.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL136666.1, SRR5189661.115172.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000358007.7/ ENSP00000350698.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCAC CCGAGGAACA CGATTCTCCG ACCGAAGCGT CCCAGCCGAT TGTGGAAGAG 
GAGGAAACTA AAACATTTAA AGACCTGGGT GTGACAGATG TGTTGTGTGA AGCTTGTGAC 
CAGTTGGGAT GGACAAAACC CACCAAGATC CAGATTGAAG CTATTCCTTT GGCCTTACAA 
GGTCGTGATA TCATTGGGCT TGCAGAAACT GGCTCTGGAA AGACAGGCGC CTTTGCTTTG 
CCCATTCTAA ACGCACTGCT GGAGACCCCG CAGCGTTTGT TTGCCCTAGT TCTTACCCCG 
ACTCGGGAGC TGGCCTTTCA GATCTCAGAG CAGTTTGAAG CCCTGGGGTC CTCTATTGGA 
GTGCAGAGTG CTGTGATTGT AGGTGGAATT GATTCAATGT CTCAATCTTT GGCCCTTGCA 
AAAAAACCAC ATATAATAAT AGCAACTCCT GGTCGACTGA TTGACCACTT GGAAAATACG 
AAAGGTTTCA ACTTGAGAGC TCTCAAATAC TTGGTCATGG ATGAAGCCGA CCGAATACTG 
AATATGGATT TTGAGACAGA GGTTGACAAG ATCCTCAAAG TGATTCCTCG AGATCGGAAA 
ACATTCCTCT TCTCTGCCAC CATGACCAAG AAGGTTCAAA AACTTCAGCG AGCAGCTCTG 
AAGAATCCTG TGAAATGTGC CGTTTCCTCT AAATACCAGA CAGTTGAAAA ATTACAGCAA 
TATTATATTT TTATTCCCTC TAAATTCAAG GATACCTACC TGGTTTATAT TCTAAATGAA 
TTGGCTGGAA ACTCCTTTAT GATATTCTGC AGCACCTGTA ATAATACCCA GAGAACAGCT 
TTGCTACTGC GAAATCTTGG CTTCACTGCC ATCCCCCTCC ATGGACAAAT GAGTCAGAGT 
AAGCGCCTAG GATCCCTTAA TAAGTTTAAG GCCAAGGCCC GTTCCATTCT TCTAGCAACT 
GACGTTGCCA GCCGAGGTTT GGACATACCT CATGTAGATG TGGTTGTCAA CTTTGACATT 
CCTACCCATT CCAAGGATTA CATCCATCGA GTAGGTCGAA CAGCTAGAGC TGGGCGCTCC 
GGAAAGGCTA TTACTTTTGT CACACAGTAT GATGTGGAAC TCTTCCAGCG CATAGAACAC 
TTAATTGGGA AGAAACTACC AGGTTTTCCA ACACAGGATG ATGAGGTTAT GATGCTGACA 
GAACGCGTCG CTGAAGCCCA AAGGTTTGCC CGAATGGAGT TAAGGGAGCA TGGAGAAAAG 
AAGAAACGCT CGCGAGAGGA TGCTGGAGAT AATGATGACA CAGAGGGTGC TATTGGTGTC 
AGGAACAAGG TGGCTGGAGG AAAAATGAAG AAGCGGAAAG GCCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057439.2
CDS	23..1390
Translation	MAAPEEHDSPTEASQPIVEEEETKTFKDLGVTDVLCEACDQLGWTKPTKIQIEAIPLALQGRDIIGLAETGSGKTGAFALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIGVQSAVIVGGIDSMSQSLALAKKPHIIIATPGRLIDHLENTKGFNLRALKYLVMDEADRILNMDFETEVDKILKVIPRDRKTFLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKDTYLVYILNELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQSKRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRSGKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEKKKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR

Target ORF information:

RefSeq Version	NM_016355.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 47 (DDX47), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016355.4

ORF Insert Sequence:

ATGGCGGCAC CCGAGGAACA CGATTCTCCG ACCGAAGCGT CCCAGCCGAT TGTGGAAGAG 
GAGGAAACTA AAACATTTAA AGACCTGGGT GTGACAGATG TGTTGTGTGA AGCTTGTGAC 
CAGTTGGGAT GGACAAAACC CACCAAGATC CAGATTGAAG CTATTCCTTT GGCCTTACAA 
GGTCGTGATA TCATTGGGCT TGCAGAAACT GGCTCTGGAA AGACAGGCGC CTTTGCTTTG 
CCCATTCTAA ACGCACTGCT GGAGACCCCG CAGCGTTTGT TTGCCCTAGT TCTTACCCCG 
ACTCGGGAGC TGGCCTTTCA GATCTCAGAG CAGTTTGAAG CCCTGGGGTC CTCTATTGGA 
GTGCAGAGTG CTGTGATTGT AGGTGGAATT GATTCAATGT CTCAATCTTT GGCCCTTGCA 
AAAAAACCAC ATATAATAAT AGCAACTCCT GGTCGACTGA TTGACCACTT GGAAAATACG 
AAAGGTTTCA ACTTGAGAGC TCTCAAATAC TTGGTCATGG ATGAAGCCGA CCGAATACTG 
AATATGGATT TTGAGACAGA GGTTGACAAG ATCCTCAAAG TGATTCCTCG AGATCGGAAA 
ACATTCCTCT TCTCTGCCAC CATGACCAAG AAGGTTCAAA AACTTCAGCG AGCAGCTCTG 
AAGAATCCTG TGAAATGTGC CGTTTCCTCT AAATACCAGA CAGTTGAAAA ATTACAGCAA 
TATTATATTT TTATTCCCTC TAAATTCAAG GATACCTACC TGGTTTATAT TCTAAATGAA 
TTGGCTGGAA ACTCCTTTAT GATATTCTGC AGCACCTGTA ATAATACCCA GAGAACAGCT 
TTGCTACTGC GAAATCTTGG CTTCACTGCC ATCCCCCTCC ATGGACAAAT GAGTCAGAGT 
AAGCGCCTAG GATCCCTTAA TAAGTTTAAG GCCAAGGCCC GTTCCATTCT TCTAGCAACT 
GACGTTGCCA GCCGAGGTTT GGACATACCT CATGTAGATG TGGTTGTCAA CTTTGACATT 
CCTACCCATT CCAAGGATTA CATCCATCGA GTAGGTCGAA CAGCTAGAGC TGGGCGCTCC 
GGAAAGGCTA TTACTTTTGT CACACAGTAT GATGTGGAAC TCTTCCAGCG CATAGAACAC 
TTAATTGGGA AGAAACTACC AGGTTTTCCA ACACAGGATG ATGAGGTTAT GATGCTGACA 
GAACGCGTCG CTGAAGCCCA AAGGTTTGCC CGAATGGAGT TAAGGGAGCA TGGAGAAAAG 
AAGAAACGCT CGCGAGAGGA TGCTGGAGAT AATGATGACA CAGAGGGTGC TATTGGTGTC 
AGGAACAAGG TGGCTGGAGG AAAAATGAAG AAGCGGAAAG GCCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX47 ( NM_201224.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_957518.1 Homo sapiens DEAD-box helicase 47 (DDX47), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02834
Clone ID Related Accession (Same CDS sequence)	NM_201224.1 , NM_201224.2
Accession Version	NM_201224.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX47 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136666.1, AF078843.1 and BC009379.2. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, as compared to variant 1. The encoded isoform (2) is thus missing an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF078843.1, SRR1803613.411701.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAC CCGAGGAACA CGATTCTCCG ACCGAAGCGT CCCAGCCGAT TGTGGAAGAG 
GAGGAAACTA AAACATTTAA AGACCTGGGT GTGACAGATG TGTTGTGTGA AGCTTGTGAC 
CAGTTGGGAT GGACAAAACC CACCAAGATC CAGATTGAAG CTATTCCTTT GGCCTTACAA 
GGTCGTGATA TCATTGGGCT TGCAGAAACT GGCTCTGGAA AGACAGGCGC CTTTGCTTTG 
CCCATTCTAA ACGCACTGCT GGAGACCCCG CAGCGTTTGT TTGCCCTAGT TCTTACCCCG 
ACTCGGGAGC TGGCCTTTCA GATCTCAGAG CAGTTTGAAG CCCTGGGGTC CTCTATTGGA 
GTGCAGAGTG CTGTGATTGT AGGTGGAATT GATTCAATGT CTCAATCTTT GGCCCTTGCA 
AAAAAACCAC ATATAATAAT AGCAACTCCT GGTCGACTGA TTGACCACTT GGAAAATACG 
AAAGGTTTCA ACTTGAGAGC TCTCAAATAC TTGGTCATGG ATGAAGCCGA CCGAATACTG 
AATATGGATT TTGAGACAGA GGTTGACAAG ATCCTCAAAG TGATTCCTCG AGATCGGAAA 
ACATTCCTCT TCTCTGCCAC CATGACCAAG AAGGTTCAAA AACTTCAGCG AGCAGCTCTG 
AAGAATCCTG TGAAATGTGC CGTTTCCTCT AAATACCAGA CAGTTGAAAA ATTACAGCAA 
TATTATATTT TTATTCCCTC TAAATTCAAG AGTAAGCGCC TAGGATCCCT TAATAAGTTT 
AAGGCCAAGG CCCGTTCCAT TCTTCTAGCA ACTGACGTTG CCAGCCGAGG TTTGGACATA 
CCTCATGTAG ATGTGGTTGT CAACTTTGAC ATTCCTACCC ATTCCAAGGA TTACATCCAT 
CGAGTAGGTC GAACAGCTAG AGCTGGGCGC TCCGGAAAGG CTATTACTTT TGTCACACAG 
TATGATGTGG AACTCTTCCA GCGCATAGAA CACTTAATTG GGAAGAAACT ACCAGGTTTT 
CCAACACAGG ATGATGAGGT TATGATGCTG ACAGAACGCG TCGCTGAAGC CCAAAGGTTT 
GCCCGAATGG AGTTAAGGGA GCATGGAGAA AAGAAGAAAC GCTCGCGAGA GGATGCTGGA 
GATAATGATG ACACAGAGGG TGCTATTGGT GTCAGGAACA AGGTGGCTGG AGGAAAAATG 
AAGAAGCGGA AAGGCCGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957518.1
CDS	23..1243
Translation	MAAPEEHDSPTEASQPIVEEEETKTFKDLGVTDVLCEACDQLGWTKPTKIQIEAIPLALQGRDIIGLAETGSGKTGAFALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIGVQSAVIVGGIDSMSQSLALAKKPHIIIATPGRLIDHLENTKGFNLRALKYLVMDEADRILNMDFETEVDKILKVIPRDRKTFLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKSKRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRSGKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEKKKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR

Target ORF information:

RefSeq Version	NM_201224.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 47 (DDX47), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201224.1

ORF Insert Sequence:

ATGGCGGCAC CCGAGGAACA CGATTCTCCG ACCGAAGCGT CCCAGCCGAT TGTGGAAGAG 
GAGGAAACTA AAACATTTAA AGACCTGGGT GTGACAGATG TGTTGTGTGA AGCTTGTGAC 
CAGTTGGGAT GGACAAAACC CACCAAGATC CAGATTGAAG CTATTCCTTT GGCCTTACAA 
GGTCGTGATA TCATTGGGCT TGCAGAAACT GGCTCTGGAA AGACAGGCGC CTTTGCTTTG 
CCCATTCTAA ACGCACTGCT GGAGACCCCG CAGCGTTTGT TTGCCCTAGT TCTTACCCCG 
ACTCGGGAGC TGGCCTTTCA GATCTCAGAG CAGTTTGAAG CCCTGGGGTC CTCTATTGGA 
GTGCAGAGTG CTGTGATTGT AGGTGGAATT GATTCAATGT CTCAATCTTT GGCCCTTGCA 
AAAAAACCAC ATATAATAAT AGCAACTCCT GGTCGACTGA TTGACCACTT GGAAAATACG 
AAAGGTTTCA ACTTGAGAGC TCTCAAATAC TTGGTCATGG ATGAAGCCGA CCGAATACTG 
AATATGGATT TTGAGACAGA GGTTGACAAG ATCCTCAAAG TGATTCCTCG AGATCGGAAA 
ACATTCCTCT TCTCTGCCAC CATGACCAAG AAGGTTCAAA AACTTCAGCG AGCAGCTCTG 
AAGAATCCTG TGAAATGTGC CGTTTCCTCT AAATACCAGA CAGTTGAAAA ATTACAGCAA 
TATTATATTT TTATTCCCTC TAAATTCAAG AGTAAGCGCC TAGGATCCCT TAATAAGTTT 
AAGGCCAAGG CCCGTTCCAT TCTTCTAGCA ACTGACGTTG CCAGCCGAGG TTTGGACATA 
CCTCATGTAG ATGTGGTTGT CAACTTTGAC ATTCCTACCC ATTCCAAGGA TTACATCCAT 
CGAGTAGGTC GAACAGCTAG AGCTGGGCGC TCCGGAAAGG CTATTACTTT TGTCACACAG 
TATGATGTGG AACTCTTCCA GCGCATAGAA CACTTAATTG GGAAGAAACT ACCAGGTTTT 
CCAACACAGG ATGATGAGGT TATGATGCTG ACAGAACGCG TCGCTGAAGC CCAAAGGTTT 
GCCCGAATGG AGTTAAGGGA GCATGGAGAA AAGAAGAAAC GCTCGCGAGA GGATGCTGGA 
GATAATGATG ACACAGAGGG TGCTATTGGT GTCAGGAACA AGGTGGCTGG AGGAAAAATG 
AAGAAGCGGA AAGGCCGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX47 ( NM_201224.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_957518.1 Homo sapiens DEAD-box helicase 47 (DDX47), transcript variant 2, mRNA.

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CloneID	OHu02834
Clone ID Related Accession (Same CDS sequence)	NM_201224.1 , NM_201224.2
Accession Version	NM_201224.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX47 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136666.1, AF078843.1 and BC009379.2. On May 31, 2019 this sequence version replaced NM_201224.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, as compared to variant 1. The encoded isoform (2) is thus missing an internal segment, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.253344.1, AF078843.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCAC CCGAGGAACA CGATTCTCCG ACCGAAGCGT CCCAGCCGAT TGTGGAAGAG 
GAGGAAACTA AAACATTTAA AGACCTGGGT GTGACAGATG TGTTGTGTGA AGCTTGTGAC 
CAGTTGGGAT GGACAAAACC CACCAAGATC CAGATTGAAG CTATTCCTTT GGCCTTACAA 
GGTCGTGATA TCATTGGGCT TGCAGAAACT GGCTCTGGAA AGACAGGCGC CTTTGCTTTG 
CCCATTCTAA ACGCACTGCT GGAGACCCCG CAGCGTTTGT TTGCCCTAGT TCTTACCCCG 
ACTCGGGAGC TGGCCTTTCA GATCTCAGAG CAGTTTGAAG CCCTGGGGTC CTCTATTGGA 
GTGCAGAGTG CTGTGATTGT AGGTGGAATT GATTCAATGT CTCAATCTTT GGCCCTTGCA 
AAAAAACCAC ATATAATAAT AGCAACTCCT GGTCGACTGA TTGACCACTT GGAAAATACG 
AAAGGTTTCA ACTTGAGAGC TCTCAAATAC TTGGTCATGG ATGAAGCCGA CCGAATACTG 
AATATGGATT TTGAGACAGA GGTTGACAAG ATCCTCAAAG TGATTCCTCG AGATCGGAAA 
ACATTCCTCT TCTCTGCCAC CATGACCAAG AAGGTTCAAA AACTTCAGCG AGCAGCTCTG 
AAGAATCCTG TGAAATGTGC CGTTTCCTCT AAATACCAGA CAGTTGAAAA ATTACAGCAA 
TATTATATTT TTATTCCCTC TAAATTCAAG AGTAAGCGCC TAGGATCCCT TAATAAGTTT 
AAGGCCAAGG CCCGTTCCAT TCTTCTAGCA ACTGACGTTG CCAGCCGAGG TTTGGACATA 
CCTCATGTAG ATGTGGTTGT CAACTTTGAC ATTCCTACCC ATTCCAAGGA TTACATCCAT 
CGAGTAGGTC GAACAGCTAG AGCTGGGCGC TCCGGAAAGG CTATTACTTT TGTCACACAG 
TATGATGTGG AACTCTTCCA GCGCATAGAA CACTTAATTG GGAAGAAACT ACCAGGTTTT 
CCAACACAGG ATGATGAGGT TATGATGCTG ACAGAACGCG TCGCTGAAGC CCAAAGGTTT 
GCCCGAATGG AGTTAAGGGA GCATGGAGAA AAGAAGAAAC GCTCGCGAGA GGATGCTGGA 
GATAATGATG ACACAGAGGG TGCTATTGGT GTCAGGAACA AGGTGGCTGG AGGAAAAATG 
AAGAAGCGGA AAGGCCGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957518.1
CDS	23..1243
Translation	MAAPEEHDSPTEASQPIVEEEETKTFKDLGVTDVLCEACDQLGWTKPTKIQIEAIPLALQGRDIIGLAETGSGKTGAFALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIGVQSAVIVGGIDSMSQSLALAKKPHIIIATPGRLIDHLENTKGFNLRALKYLVMDEADRILNMDFETEVDKILKVIPRDRKTFLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKSKRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRSGKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEKKKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR

Target ORF information:

RefSeq Version	NM_201224.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 47 (DDX47), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201224.2

ORF Insert Sequence:

ATGGCGGCAC CCGAGGAACA CGATTCTCCG ACCGAAGCGT CCCAGCCGAT TGTGGAAGAG 
GAGGAAACTA AAACATTTAA AGACCTGGGT GTGACAGATG TGTTGTGTGA AGCTTGTGAC 
CAGTTGGGAT GGACAAAACC CACCAAGATC CAGATTGAAG CTATTCCTTT GGCCTTACAA 
GGTCGTGATA TCATTGGGCT TGCAGAAACT GGCTCTGGAA AGACAGGCGC CTTTGCTTTG 
CCCATTCTAA ACGCACTGCT GGAGACCCCG CAGCGTTTGT TTGCCCTAGT TCTTACCCCG 
ACTCGGGAGC TGGCCTTTCA GATCTCAGAG CAGTTTGAAG CCCTGGGGTC CTCTATTGGA 
GTGCAGAGTG CTGTGATTGT AGGTGGAATT GATTCAATGT CTCAATCTTT GGCCCTTGCA 
AAAAAACCAC ATATAATAAT AGCAACTCCT GGTCGACTGA TTGACCACTT GGAAAATACG 
AAAGGTTTCA ACTTGAGAGC TCTCAAATAC TTGGTCATGG ATGAAGCCGA CCGAATACTG 
AATATGGATT TTGAGACAGA GGTTGACAAG ATCCTCAAAG TGATTCCTCG AGATCGGAAA 
ACATTCCTCT TCTCTGCCAC CATGACCAAG AAGGTTCAAA AACTTCAGCG AGCAGCTCTG 
AAGAATCCTG TGAAATGTGC CGTTTCCTCT AAATACCAGA CAGTTGAAAA ATTACAGCAA 
TATTATATTT TTATTCCCTC TAAATTCAAG AGTAAGCGCC TAGGATCCCT TAATAAGTTT 
AAGGCCAAGG CCCGTTCCAT TCTTCTAGCA ACTGACGTTG CCAGCCGAGG TTTGGACATA 
CCTCATGTAG ATGTGGTTGT CAACTTTGAC ATTCCTACCC ATTCCAAGGA TTACATCCAT 
CGAGTAGGTC GAACAGCTAG AGCTGGGCGC TCCGGAAAGG CTATTACTTT TGTCACACAG 
TATGATGTGG AACTCTTCCA GCGCATAGAA CACTTAATTG GGAAGAAACT ACCAGGTTTT 
CCAACACAGG ATGATGAGGT TATGATGCTG ACAGAACGCG TCGCTGAAGC CCAAAGGTTT 
GCCCGAATGG AGTTAAGGGA GCATGGAGAA AAGAAGAAAC GCTCGCGAGA GGATGCTGGA 
GATAATGATG ACACAGAGGG TGCTATTGGT GTCAGGAACA AGGTGGCTGG AGGAAAAATG 
AAGAAGCGGA AAGGCCGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Protein microarray analysis identifies human cellular prion protein interactors.
Neuropathology and applied neurobiology35(1)16-35(2009 Feb)
Satoh J,Obayashi S,Misawa T,Sumiyoshi K,Oosumi K,Tabunoki H

Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.
EMBO reports8(11)1052-60(2007 Nov)
H?ck J,Weinmann L,Ender C,R?del S,Kremmer E,Raabe M,Urlaub H,Meister G

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DDX47 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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