DND1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DND1
Entrez Gene ID	373863
Full Name	DND microRNA-mediated repression inhibitor 1
Synonyms	RBMS4
General protein information	Preferred Names DND microRNA-mediated repression inhibitor 1 Names dead end protein homolog 1 RNA-binding motif, single-stranded-interacting protein 4 dead end homolog 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.3
Summary	This gene encodes a protein that binds to microRNA-targeting sequences of mRNAs, inhibiting microRNA-mediated repression. Reduced expression of this gene has been implicated in tongue squamous cell carcinoma. Two pseudogenes of this gene are located on the long arm of chromosome 17. [provided by RefSeq, Dec 2010].
Disorder MIM:	609385

mRNA and Protein(s)

mRNA	Protein	Name
NM_194249.2	NP_919225.1	dead end protein homolog 1
NM_194249.3	NP_919225.1	dead end protein homolog 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dnd1	NP_001102849.1
Homo sapiens (human)	DND1	NP_919225.1
Gallus gallus (chicken)	DND1	XP_423051.4
Bos taurus (cattle)	DND1	NP_001180074.1
Mus musculus (house mouse)	Dnd1	NP_775559.2
Xenopus tropicalis (tropical clawed frog)	dnd1	NP_001037899.1
Pan troglodytes (chimpanzee)	DND1	XP_517978.2
Canis lupus familiaris (dog)	DND1	XP_848834.1

Gene Ontology
Bibliography

Related articles in PubMed

RNA-binding protein Dnd1 inhibits epithelial-mesenchymal transition and cancer stem cell-related traits on hepatocellular carcinoma cells.
Xu W, Gong F, Zhang T, Chi B, Wang J
Biotechnology letters39(9)1359-1367(2017 Sep)

RNA-Binding Protein Dnd1 Promotes Breast Cancer Apoptosis by Stabilizing the Bim mRNA in a miR-221 Binding Site.
Cheng F, Pan Y, Lu YM, Zhu L, Chen S
BioMed research international20179596152(2017)

The RNA binding proteins RBM38 and DND1 are repressed in AML and have a novel function in APL differentiation.
Wampfler J, Federzoni EA, Torbett BE, Fey MF, Tschan MP
Leukemia research4196-102(2016 Feb)

APOBEC3 inhibits DEAD-END function to regulate microRNA activity.
Ali S, Karki N, Bhattacharya C, Zhu R, MacDuff DA, Stenglein MD, Schumacher AJ, Demorest ZL, Harris RS, Matin A, Aggarwal S
BMC molecular biology1416(2013 Jul)

The Grainyhead transcription factor Grhl3/Get1 suppresses miR-21 expression and tumorigenesis in skin: modulation of the miR-21 target MSH2 by RNA-binding protein DND1.
Bhandari A, Gordon W, Dizon D, Hopkin AS, Gordon E, Yu Z, Andersen B
Oncogene32(12)1497-507(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dnd1 facilitates apoptosis by increasing the expression of Bim via its competitive combining with miR-221 in Bim-3'UTR.
Title: RNA-Binding Protein Dnd1 Promotes Breast Cancer Apoptosis by Stabilizing the Bim mRNA in a miR-221 Binding Site.

RBM38 and DND1 are repressed in primary acute myeloid leukemia, neutrophil differentiation is dependent on increased expression of both proteins, and they have a role in regulating p21(CIP1) expression during acute promyelocytic leukemia differentiation
Title: The RNA binding proteins RBM38 and DND1 are repressed in AML and have a novel function in APL differentiation.

APOBEC3G inhibits DND1 function to regulate microRNA activity.
Title: APOBEC3 inhibits DEAD-END function to regulate microRNA activity.

The increased sensitivity of transformed keratinocytes to miR-21's effects occurs in part through downregulation of the RNA-binding protein DND1 during the transformation process.
Title: The Grainyhead transcription factor Grhl3/Get1 suppresses miR-21 expression and tumorigenesis in skin: modulation of the miR-21 target MSH2 by RNA-binding protein DND1.

Results suggest that DND1 may impose another level of translational regulation to modulate expression of critical factors in ES cells.
Title: Transcripts that associate with the RNA binding protein, DEAD-END (DND1), in embryonic stem (ES) cells.

The following DND1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DND1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12248	NM_194249.3 Latest version!	Homo sapiens DND microRNA-mediated repression inhibitor 1 (DND1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
View Old Accession Versions >>
OHu12248	NM_194249.2	Homo sapiens DND microRNA-mediated repression inhibitor 1 (DND1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DND1 ( NM_194249.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_919225.1 Homo sapiens DND microRNA-mediated repression inhibitor 1 (DND1), mRNA.

Price & Availability↑

CloneID	OHu12248
Clone ID Related Accession (Same CDS sequence)	NM_194249.2 , NM_194249.3
Accession Version	NM_194249.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	dead end protein homolog 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC033496.1 and AC116353.2. On Dec 4, 2010 this sequence version replaced NM_194249.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033496.1, AY321065.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTCCA AGCGGGATTG TGAGCTGTGG TGTGAGAGGG TGAATCCAGA GAACAAGGCG 
GCGCTGGAGG CGTGGGTCAG GGAGACAGGC ATCCGCCTGG TGCAGGTGAA CGGGCAGAGG 
AAGTATGGCG GGCCACCCCC AGGCTGGGTG GGCAGCCCGC CGCCAGCTGG GTCAGAGGTG 
TTCATCGGGC GGCTGCCTCA GGACGTGTAC GAGCACCAGC TTATCCCGCT GTTCCAGCGC 
GTGGGCCGCC TCTACGAGTT CCGCCTGATG ATGACCTTCA GCGGCCTGAA CCGCGGCTTC 
GCCTATGCCC GCTACAGCTC GAGGCGCGGC GCGCAGGCCG CCATCGCCAC GCTGCACAAC 
CATCCGCTGC GGCCGTCCTG CCCGCTGCTC GTGTGCCGCA GCACCGAGAA GTGTGAGCTG 
AGCGTTGACG GCCTGCCGCC GAATCTGACC CGCAGCGCGC TGCTGCTCGC GCTGCAGCCG 
CTGGGTCCCG GCTTGCAGGA GGCGCGGCTG CTGCCCAGCC CCGGACCGGC GCCCGGGCAG 
ATCGCTCTGC TCAAATTCAG CTCGCACCGG GCCGCTGCCA TGGCCAAAAA GGCCCTGGTG 
GAAGGGCAGT CACACCTCTG TGGAGAGCAG GTGGCTGTGG AGTGGCTCAA GCCAGACCTG 
AAGCAGCGAC TTCGCCAGCA GCTTGTGGGT CCCTTCTTGC GGTCCCCACA GCCAGAGGGC 
AGCCAGTTGG CTTTGGCAAG GGACAAGTTA GGGTTCCAAG GGGCTCGGGC TACCCTGCAG 
TTGCTGTGCC AACGAATGAA GCTGGGCAGC CCTGTGTTCC TCACCAAGTG TTTGGGCATA 
GGACCTGCTG GCTGGCACCG CTTCTGGTAC CAGGTGGTGA TTCCTGGGCA TCCGGTGCCC 
TTCAGCGGCC TCATCTGGGT TGTGCTGACC CTAGATGGCC GGGATGGGCA TGAGGTGGCC 
AAGGATGCTG TGTCTGTACG GCTGCTGCAG GCACTCAGTG AGTCTGGGGC CAACCTCCTG 
TGGTCTGCTG GGGCTGAGGC AGGTACCATG GTTAAACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919225.1
CDS	45..1106
Translation	MQSKRDCELWCERVNPENKAALEAWVRETGIRLVQVNGQRKYGGPPPGWVGSPPPAGSEVFIGRLPQDVYEHQLIPLFQRVGRLYEFRLMMTFSGLNRGFAYARYSSRRGAQAAIATLHNHPLRPSCPLLVCRSTEKCELSVDGLPPNLTRSALLLALQPLGPGLQEARLLPSPGPAPGQIALLKFSSHRAAAMAKKALVEGQSHLCGEQVAVEWLKPDLKQRLRQQLVGPFLRSPQPEGSQLALARDKLGFQGARATLQLLCQRMKLGSPVFLTKCLGIGPAGWHRFWYQVVIPGHPVPFSGLIWVVLTLDGRDGHEVAKDAVSVRLLQALSESGANLLWSAGAEAGTMVKQ

Target ORF information:

RefSeq Version	NM_194249.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DND microRNA-mediated repression inhibitor 1 (DND1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194249.2

ORF Insert Sequence:

ATGCAGTCCA AGCGGGATTG TGAGCTGTGG TGTGAGAGGG TGAATCCAGA GAACAAGGCG 
GCGCTGGAGG CGTGGGTCAG GGAGACAGGC ATCCGCCTGG TGCAGGTGAA CGGGCAGAGG 
AAGTATGGCG GGCCACCCCC AGGCTGGGTG GGCAGCCCGC CGCCAGCTGG GTCAGAGGTG 
TTCATCGGGC GGCTGCCTCA GGACGTGTAC GAGCACCAGC TTATCCCGCT GTTCCAGCGC 
GTGGGCCGCC TCTACGAGTT CCGCCTGATG ATGACCTTCA GCGGCCTGAA CCGCGGCTTC 
GCCTATGCCC GCTACAGCTC GAGGCGCGGC GCGCAGGCCG CCATCGCCAC GCTGCACAAC 
CATCCGCTGC GGCCGTCCTG CCCGCTGCTC GTGTGCCGCA GCACCGAGAA GTGTGAGCTG 
AGCGTTGACG GCCTGCCGCC GAATCTGACC CGCAGCGCGC TGCTGCTCGC GCTGCAGCCG 
CTGGGTCCCG GCTTGCAGGA GGCGCGGCTG CTGCCCAGCC CCGGACCGGC GCCCGGGCAG 
ATCGCTCTGC TCAAATTCAG CTCGCACCGG GCCGCTGCCA TGGCCAAAAA GGCCCTGGTG 
GAAGGGCAGT CACACCTCTG TGGAGAGCAG GTGGCTGTGG AGTGGCTCAA GCCAGACCTG 
AAGCAGCGAC TTCGCCAGCA GCTTGTGGGT CCCTTCTTGC GGTCCCCACA GCCAGAGGGC 
AGCCAGTTGG CTTTGGCAAG GGACAAGTTA GGGTTCCAAG GGGCTCGGGC TACCCTGCAG 
TTGCTGTGCC AACGAATGAA GCTGGGCAGC CCTGTGTTCC TCACCAAGTG TTTGGGCATA 
GGACCTGCTG GCTGGCACCG CTTCTGGTAC CAGGTGGTGA TTCCTGGGCA TCCGGTGCCC 
TTCAGCGGCC TCATCTGGGT TGTGCTGACC CTAGATGGCC GGGATGGGCA TGAGGTGGCC 
AAGGATGCTG TGTCTGTACG GCTGCTGCAG GCACTCAGTG AGTCTGGGGC CAACCTCCTG 
TGGTCTGCTG GGGCTGAGGC AGGTACCATG GTTAAACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DND1 ( NM_194249.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_919225.1 Homo sapiens DND microRNA-mediated repression inhibitor 1 (DND1), mRNA.

Price & Availability↑

CloneID	OHu12248
Clone ID Related Accession (Same CDS sequence)	NM_194249.2 , NM_194249.3
Accession Version	NM_194249.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	dead end protein homolog 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC033496.1 and AC116353.2. On Nov 22, 2018 this sequence version replaced NM_194249.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033496.1, AY321065.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000542735.2/ ENSP00000445366.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGTCCA AGCGGGATTG TGAGCTGTGG TGTGAGAGGG TGAATCCAGA GAACAAGGCG 
GCGCTGGAGG CGTGGGTCAG GGAGACAGGC ATCCGCCTGG TGCAGGTGAA CGGGCAGAGG 
AAGTATGGCG GGCCACCCCC AGGCTGGGTG GGCAGCCCGC CGCCAGCTGG GTCAGAGGTG 
TTCATCGGGC GGCTGCCTCA GGACGTGTAC GAGCACCAGC TTATCCCGCT GTTCCAGCGC 
GTGGGCCGCC TCTACGAGTT CCGCCTGATG ATGACCTTCA GCGGCCTGAA CCGCGGCTTC 
GCCTATGCCC GCTACAGCTC GAGGCGCGGC GCGCAGGCCG CCATCGCCAC GCTGCACAAC 
CATCCGCTGC GGCCGTCCTG CCCGCTGCTC GTGTGCCGCA GCACCGAGAA GTGTGAGCTG 
AGCGTTGACG GCCTGCCGCC GAATCTGACC CGCAGCGCGC TGCTGCTCGC GCTGCAGCCG 
CTGGGTCCCG GCTTGCAGGA GGCGCGGCTG CTGCCCAGCC CCGGACCGGC GCCCGGGCAG 
ATCGCTCTGC TCAAATTCAG CTCGCACCGG GCCGCTGCCA TGGCCAAAAA GGCCCTGGTG 
GAAGGGCAGT CACACCTCTG TGGAGAGCAG GTGGCTGTGG AGTGGCTCAA GCCAGACCTG 
AAGCAGCGAC TTCGCCAGCA GCTTGTGGGT CCCTTCTTGC GGTCCCCACA GCCAGAGGGC 
AGCCAGTTGG CTTTGGCAAG GGACAAGTTA GGGTTCCAAG GGGCTCGGGC TACCCTGCAG 
TTGCTGTGCC AACGAATGAA GCTGGGCAGC CCTGTGTTCC TCACCAAGTG TTTGGGCATA 
GGACCTGCTG GCTGGCACCG CTTCTGGTAC CAGGTGGTGA TTCCTGGGCA TCCGGTGCCC 
TTCAGCGGCC TCATCTGGGT TGTGCTGACC CTAGATGGCC GGGATGGGCA TGAGGTGGCC 
AAGGATGCTG TGTCTGTACG GCTGCTGCAG GCACTCAGTG AGTCTGGGGC CAACCTCCTG 
TGGTCTGCTG GGGCTGAGGC AGGTACCATG GTTAAACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919225.1
CDS	35..1096
Translation	MQSKRDCELWCERVNPENKAALEAWVRETGIRLVQVNGQRKYGGPPPGWVGSPPPAGSEVFIGRLPQDVYEHQLIPLFQRVGRLYEFRLMMTFSGLNRGFAYARYSSRRGAQAAIATLHNHPLRPSCPLLVCRSTEKCELSVDGLPPNLTRSALLLALQPLGPGLQEARLLPSPGPAPGQIALLKFSSHRAAAMAKKALVEGQSHLCGEQVAVEWLKPDLKQRLRQQLVGPFLRSPQPEGSQLALARDKLGFQGARATLQLLCQRMKLGSPVFLTKCLGIGPAGWHRFWYQVVIPGHPVPFSGLIWVVLTLDGRDGHEVAKDAVSVRLLQALSESGANLLWSAGAEAGTMVKQ

Target ORF information:

RefSeq Version	NM_194249.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DND microRNA-mediated repression inhibitor 1 (DND1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194249.3

ORF Insert Sequence:

ATGCAGTCCA AGCGGGATTG TGAGCTGTGG TGTGAGAGGG TGAATCCAGA GAACAAGGCG 
GCGCTGGAGG CGTGGGTCAG GGAGACAGGC ATCCGCCTGG TGCAGGTGAA CGGGCAGAGG 
AAGTATGGCG GGCCACCCCC AGGCTGGGTG GGCAGCCCGC CGCCAGCTGG GTCAGAGGTG 
TTCATCGGGC GGCTGCCTCA GGACGTGTAC GAGCACCAGC TTATCCCGCT GTTCCAGCGC 
GTGGGCCGCC TCTACGAGTT CCGCCTGATG ATGACCTTCA GCGGCCTGAA CCGCGGCTTC 
GCCTATGCCC GCTACAGCTC GAGGCGCGGC GCGCAGGCCG CCATCGCCAC GCTGCACAAC 
CATCCGCTGC GGCCGTCCTG CCCGCTGCTC GTGTGCCGCA GCACCGAGAA GTGTGAGCTG 
AGCGTTGACG GCCTGCCGCC GAATCTGACC CGCAGCGCGC TGCTGCTCGC GCTGCAGCCG 
CTGGGTCCCG GCTTGCAGGA GGCGCGGCTG CTGCCCAGCC CCGGACCGGC GCCCGGGCAG 
ATCGCTCTGC TCAAATTCAG CTCGCACCGG GCCGCTGCCA TGGCCAAAAA GGCCCTGGTG 
GAAGGGCAGT CACACCTCTG TGGAGAGCAG GTGGCTGTGG AGTGGCTCAA GCCAGACCTG 
AAGCAGCGAC TTCGCCAGCA GCTTGTGGGT CCCTTCTTGC GGTCCCCACA GCCAGAGGGC 
AGCCAGTTGG CTTTGGCAAG GGACAAGTTA GGGTTCCAAG GGGCTCGGGC TACCCTGCAG 
TTGCTGTGCC AACGAATGAA GCTGGGCAGC CCTGTGTTCC TCACCAAGTG TTTGGGCATA 
GGACCTGCTG GCTGGCACCG CTTCTGGTAC CAGGTGGTGA TTCCTGGGCA TCCGGTGCCC 
TTCAGCGGCC TCATCTGGGT TGTGCTGACC CTAGATGGCC GGGATGGGCA TGAGGTGGCC 
AAGGATGCTG TGTCTGTACG GCTGCTGCAG GCACTCAGTG AGTCTGGGGC CAACCTCCTG 
TGGTCTGCTG GGGCTGAGGC AGGTACCATG GTTAAACAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNA-binding protein Dnd1 inhibits epithelial-mesenchymal transition and cancer stem cell-related traits on hepatocellular carcinoma cells.
Biotechnology letters39(9)1359-1367(2017 Sep)
Xu W,Gong F,Zhang T,Chi B,Wang J

RNA-Binding Protein Dnd1 Promotes Breast Cancer Apoptosis by Stabilizing the Bim mRNA in a miR-221 Binding Site.
BioMed research international20179596152(2017)
Cheng F,Pan Y,Lu YM,Zhu L,Chen S

The RNA binding proteins RBM38 and DND1 are repressed in AML and have a novel function in APL differentiation.
Leukemia research4196-102(2016 Feb)
Wampfler J,Federzoni EA,Torbett BE,Fey MF,Tschan MP

APOBEC3 inhibits DEAD-END function to regulate microRNA activity.
BMC molecular biology1416(2013 Jul)
Ali S,Karki N,Bhattacharya C,Zhu R,MacDuff DA,Stenglein MD,Schumacher AJ,Demorest ZL,Harris RS,Matin A,Aggarwal S

The Grainyhead transcription factor Grhl3/Get1 suppresses miR-21 expression and tumorigenesis in skin: modulation of the miR-21 target MSH2 by RNA-binding protein DND1.
Oncogene32(12)1497-507(2013 Mar)
Bhandari A,Gordon W,Dizon D,Hopkin AS,Gordon E,Yu Z,Andersen B