ITK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ITK
Entrez Gene ID	3702
Full Name	IL2 inducible T cell kinase
Synonyms	EMT,LPFS1,LYK,PSCTK2
General protein information	Preferred Names IL2 inducible T cell kinase Names tyrosine-protein kinase ITK/TSK IL-2-inducible T-cell kinase T-cell-specific kinase homolog of mouse T-cell itk/tsk interleukin-2-inducible T-cell kinase kinase EMT tyrosine-protein kinase LYK
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q33.3
Summary	This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008].
Disorder MIM:	186973
Disorder Html:	Lymphoproliferative syndrome 1, 613011 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017009443.1	XP_016864932.1	tyrosine-protein kinase ITK/TSK isoform X1
NM_005546.3	NP_005537.3	tyrosine-protein kinase ITK/TSK
NM_005546.4	NP_005537.3	tyrosine-protein kinase ITK/TSK

Pathways from BioSystems

Homologies

Homo sapiens (human)	ITK	NP_005537.3
Pan troglodytes (chimpanzee)	ITK	XP_001136073.1
Bos taurus (cattle)	ITK	NP_001098858.1
Canis lupus familiaris (dog)	ITK	XP_546277.3
Mus musculus (house mouse)	Itk	NP_001268894.1
	ITK	XP_001113468.1
Danio rerio (zebrafish)	itk	NP_571179.1
Rattus norvegicus (Norway rat)	Itk	NP_001102295.1
Gallus gallus (chicken)	ITK	XP_414568.3

Gene Ontology
Bibliography

Related articles in PubMed

ITK signalling via the Ras/IRF4 pathway regulates the development and function of Tr1 cells.
Huang W, Solouki S, Koylass N, Zheng SG, August A
Nature communications815871(2017 Jun)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Inducible T Cell Kinase Regulates the Acquisition of Cytolytic Capacity and Degranulation in CD8
Kapnick SM, Stinchcombe JC, Griffiths GM, Schwartzberg PL
Journal of immunology (Baltimore, Md. : 1950)198(7)2699-2711(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results provide new insight into the effect of ITK and suboptimal T-cell receptor signaling on CD8(+) T cell function, and how these may contribute to phenotypes associated with ITK deficiency
Title: Inducible T Cell Kinase Regulates the Acquisition of Cytolytic Capacity and Degranulation in CD8

The data indicate that increased ITK expression could act as a disease activity marker and as a risk factor for involvement in SLE, but it still needs further study to confirm.
Title: Interleukin-2-inducible T-cell kinase expression and relation to disease severity in systemic lupus erythematosus.

We conclude that ITK, formerly considered an immune cell-specific protein, is aberrantly expressed in melanoma and promotes tumor development and progression
Title: IL2 Inducible T-cell Kinase, a Novel Therapeutic Target in Melanoma.

These data indicate that PRN694 is a highly selective and potent covalent inhibitor of ITK and RLK, and its extended target residence time enables durable attenuation of effector cells in vitro and in vivo.
Title: Targeting interleukin-2-inducible T-cell kinase (ITK) and resting lymphocyte kinase (RLK) using a novel covalent inhibitor PRN694.

approach identified 18 kinase and kinase-related genes whose overexpression can substitute for EGFR in EGFR-dependent PC9 cells, and these genes include seven of nine Src family kinase genes, FGFR1, FGFR2, ITK, NTRK1, NTRK2, MOS, MST1R, and RAF1.
Title: Genetic modifiers of EGFR dependence in non-small cell lung cancer.

The following ITK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu95866	XM_017009443.1 Latest version!	Homo sapiens IL2 inducible T cell kinase (ITK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu18356	NM_005546.4 Latest version!	Homo sapiens IL2 inducible T cell kinase (ITK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu18356	NM_005546.3	Homo sapiens IL2 inducible T cell kinase (ITK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITK ( NM_005546.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005537.3 Homo sapiens IL2 inducible T cell kinase (ITK), mRNA.

Price & Availability↑

CloneID	OHu18356
Clone ID Related Accession (Same CDS sequence)	NM_005546.3 , NM_005546.4
Accession Version	NM_005546.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase ITK/TSK
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D13720.1. This sequence is a reference standard in the RefSeqGene project. On Jun 26, 2002 this sequence version replaced NM_005546.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D13720.1, BC109077.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACAACT TTATCCTCCT GGAAGAACAG CTCATCAAGA AATCCCAACA AAAGAGAAGA 
ACTTCTCCCT CGAACTTTAA AGTCCGCTTC TTTGTGTTAA CCAAAGCCAG CCTGGCATAC 
TTTGAAGATC GTCATGGGAA GAAGCGCACG CTGAAGGGGT CCATTGAGCT CTCCCGAATC 
AAATGTGTTG AGATTGTGAA AAGTGACATC AGCATCCCAT GCCACTATAA ATACCCGTTT 
CAGGTGGTGC ATGACAACTA CCTCCTATAT GTGTTTGCTC CAGATCGTGA GAGCCGGCAG 
CGCTGGGTGC TGGCCCTTAA AGAAGAAACG AGGAATAATA ACAGTTTGGT GCCTAAATAT 
CATCCTAATT TCTGGATGGA TGGGAAGTGG AGGTGCTGTT CTCAGCTGGA GAAGCTTGCA 
ACAGGCTGTG CCCAATATGA TCCAACCAAG AATGCTTCAA AGAAGCCTCT TCCTCCTACT 
CCTGAAGACA ACAGGCGACC ACTTTGGGAA CCTGAAGAAA CTGTGGTCAT TGCCTTATAT 
GACTACCAAA CCAATGATCC TCAGGAACTC GCACTGCGGC GCAACGAAGA GTACTGCCTG 
CTGGACAGTT CTGAGATTCA CTGGTGGAGA GTCCAGGACA GGAATGGGCA TGAAGGATAT 
GTACCAAGCA GTTATCTGGT GGAAAAATCT CCAAATAATC TGGAAACCTA TGAGTGGTAC 
AATAAGAGTA TCAGCCGAGA CAAAGCTGAA AAACTTCTTT TGGACACAGG CAAAGAAGGA 
GCCTTCATGG TAAGGGATTC CAGGACTGCA GGAACATACA CCGTGTCTGT TTTCACCAAG 
GCTGTTGTAA GTGAGAACAA TCCCTGTATA AAGCATTATC ACATCAAGGA AACAAATGAC 
AATCCTAAGC GATACTATGT GGCTGAAAAG TATGTGTTCG ATTCCATCCC TCTTCTCATC 
AACTATCACC AACATAATGG AGGAGGCCTG GTGACTCGAC TCCGGTATCC AGTTTGTTTT 
GGGAGGCAGA AAGCCCCAGT TACAGCAGGG CTGAGATACG GGAAATGGGT GATCGACCCC 
TCAGAGCTCA CTTTTGTGCA AGAGATTGGC AGTGGGCAAT TTGGGTTGGT GCATCTGGGC 
TACTGGCTCA ACAAGGACAA GGTGGCTATC AAAACCATTC GGGAAGGGGC TATGTCAGAA 
GAGGACTTCA TAGAGGAGGC TGAAGTAATG ATGAAACTCT CTCATCCCAA ACTGGTGCAG 
CTGTATGGGG TGTGCCTGGA GCAGGCCCCC ATCTGCCTGG TGTTTGAGTT CATGGAGCAC 
GGCTGCCTGT CAGATTATCT ACGCACCCAG CGGGGACTTT TTGCTGCAGA GACCCTGCTG 
GGCATGTGTC TGGATGTGTG TGAGGGCATG GCCTACCTGG AAGAGGCATG TGTCATCCAC 
AGAGACTTGG CTGCCAGAAA TTGTTTGGTG GGAGAAAACC AAGTCATCAA GGTGTCTGAC 
TTTGGGATGA CAAGGTTCGT TCTGGATGAT CAGTACACCA GTTCCACAGG CACCAAATTC 
CCGGTGAAGT GGGCATCCCC AGAGGTTTTC TCTTTCAGTC GCTATAGCAG CAAGTCCGAT 
GTGTGGTCAT TTGGTGTGCT GATGTGGGAA GTTTTCAGTG AAGGCAAAAT CCCGTATGAA 
AACCGAAGCA ACTCAGAGGT GGTGGAAGAC ATCAGTACCG GATTTCGGTT GTACAAGCCC 
CGGCTGGCCT CCACACACGT CTACCAGATT ATGAATCACT GCTGGAAAGA GAGACCAGAA 
GATCGGCCAG CCTTCTCCAG ACTGCTGCGT CAACTGGCTG AAATTGCAGA ATCAGGACTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005537.3
CDS	83..1945
Translation	MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSDISIPCHYKYPFQVVHDNYLLYVFAPDRESRQRWVLALKEETRNNNSLVPKYHPNFWMDGKWRCCSQLEKLATGCAQYDPTKNASKKPLPPTPEDNRRPLWEPEETVVIALYDYQTNDPQELALRRNEEYCLLDSSEIHWWRVQDRNGHEGYVPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTAGTYTVSVFTKAVVSENNPCIKHYHIKETNDNPKRYYVAEKYVFDSIPLLINYHQHNGGGLVTRLRYPVCFGRQKAPVTAGLRYGKWVIDPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIREGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRTQRGLFAAETLLGMCLDVCEGMAYLEEACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASTHVYQIMNHCWKERPEDRPAFSRLLRQLAEIAESGL

Target ORF information:

RefSeq Version	NM_005546.3
Organism	Homo sapiens(human)
Definition	Homo sapiens IL2 inducible T cell kinase (ITK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005546.3

ORF Insert Sequence:

ATGAACAACT TTATCCTCCT GGAAGAACAG CTCATCAAGA AATCCCAACA AAAGAGAAGA 
ACTTCTCCCT CGAACTTTAA AGTCCGCTTC TTTGTGTTAA CCAAAGCCAG CCTGGCATAC 
TTTGAAGATC GTCATGGGAA GAAGCGCACG CTGAAGGGGT CCATTGAGCT CTCCCGAATC 
AAATGTGTTG AGATTGTGAA AAGTGACATC AGCATCCCAT GCCACTATAA ATACCCGTTT 
CAGGTGGTGC ATGACAACTA CCTCCTATAT GTGTTTGCTC CAGATCGTGA GAGCCGGCAG 
CGCTGGGTGC TGGCCCTTAA AGAAGAAACG AGGAATAATA ACAGTTTGGT GCCTAAATAT 
CATCCTAATT TCTGGATGGA TGGGAAGTGG AGGTGCTGTT CTCAGCTGGA GAAGCTTGCA 
ACAGGCTGTG CCCAATATGA TCCAACCAAG AATGCTTCAA AGAAGCCTCT TCCTCCTACT 
CCTGAAGACA ACAGGCGACC ACTTTGGGAA CCTGAAGAAA CTGTGGTCAT TGCCTTATAT 
GACTACCAAA CCAATGATCC TCAGGAACTC GCACTGCGGC GCAACGAAGA GTACTGCCTG 
CTGGACAGTT CTGAGATTCA CTGGTGGAGA GTCCAGGACA GGAATGGGCA TGAAGGATAT 
GTACCAAGCA GTTATCTGGT GGAAAAATCT CCAAATAATC TGGAAACCTA TGAGTGGTAC 
AATAAGAGTA TCAGCCGAGA CAAAGCTGAA AAACTTCTTT TGGACACAGG CAAAGAAGGA 
GCCTTCATGG TAAGGGATTC CAGGACTGCA GGAACATACA CCGTGTCTGT TTTCACCAAG 
GCTGTTGTAA GTGAGAACAA TCCCTGTATA AAGCATTATC ACATCAAGGA AACAAATGAC 
AATCCTAAGC GATACTATGT GGCTGAAAAG TATGTGTTCG ATTCCATCCC TCTTCTCATC 
AACTATCACC AACATAATGG AGGAGGCCTG GTGACTCGAC TCCGGTATCC AGTTTGTTTT 
GGGAGGCAGA AAGCCCCAGT TACAGCAGGG CTGAGATACG GGAAATGGGT GATCGACCCC 
TCAGAGCTCA CTTTTGTGCA AGAGATTGGC AGTGGGCAAT TTGGGTTGGT GCATCTGGGC 
TACTGGCTCA ACAAGGACAA GGTGGCTATC AAAACCATTC GGGAAGGGGC TATGTCAGAA 
GAGGACTTCA TAGAGGAGGC TGAAGTAATG ATGAAACTCT CTCATCCCAA ACTGGTGCAG 
CTGTATGGGG TGTGCCTGGA GCAGGCCCCC ATCTGCCTGG TGTTTGAGTT CATGGAGCAC 
GGCTGCCTGT CAGATTATCT ACGCACCCAG CGGGGACTTT TTGCTGCAGA GACCCTGCTG 
GGCATGTGTC TGGATGTGTG TGAGGGCATG GCCTACCTGG AAGAGGCATG TGTCATCCAC 
AGAGACTTGG CTGCCAGAAA TTGTTTGGTG GGAGAAAACC AAGTCATCAA GGTGTCTGAC 
TTTGGGATGA CAAGGTTCGT TCTGGATGAT CAGTACACCA GTTCCACAGG CACCAAATTC 
CCGGTGAAGT GGGCATCCCC AGAGGTTTTC TCTTTCAGTC GCTATAGCAG CAAGTCCGAT 
GTGTGGTCAT TTGGTGTGCT GATGTGGGAA GTTTTCAGTG AAGGCAAAAT CCCGTATGAA 
AACCGAAGCA ACTCAGAGGT GGTGGAAGAC ATCAGTACCG GATTTCGGTT GTACAAGCCC 
CGGCTGGCCT CCACACACGT CTACCAGATT ATGAATCACT GCTGGAAAGA GAGACCAGAA 
GATCGGCCAG CCTTCTCCAG ACTGCTGCGT CAACTGGCTG AAATTGCAGA ATCAGGACTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITK ( XM_017009443.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864932.1 Homo sapiens IL2 inducible T cell kinase (ITK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu95866
Clone ID Related Accession (Same CDS sequence)	XM_017009443.1
Accession Version	XM_017009443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase ITK/TSK isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA AGTGGAGGTG CTGTTCTCAG CTGGAGAAGC TTGCAACAGG CTGTGCCCAA 
TATGATCCAA CCAAGAATGC TTCAAAGAAG CCTCTTCCTC CTACTCCTGA AGACAACAGG 
CGACCACTTT GGGAACCTGA AGAAACTGTG GTCATTGCCT TATATGACTA CCAAACCAAT 
GATCCTCAGG AACTCGCACT GCGGCGCAAC GAAGAGTACT GCCTGCTGGA CAGTTCTGAG 
ATTCACTGGT GGAGAGTCCA GGACAGGAAT GGGCATGAAG GATATGTACC AAGCAGTTAT 
CTGGTGGAAA AATCTCCAAA TAATCTGGAA ACCTATGAGT GGTACAATAA GAGTATCAGC 
CGAGACAAAG CTGAAAAACT TCTTTTGGAC ACAGGCAAAG AAGGAGCCTT CATGGTAAGG 
GATTCCAGGA CTGCAGGAAC ATACACCGTG TCTGTTTTCA CCAAGGCTGT TGTAAGTGAG 
AACAATCCCT GTATAAAGCA TTATCACATC AAGGAAACAA ATGACAATCC TAAGCGATAC 
TATGTGGCTG AAAAGTATGT GTTCGATTCC ATCCCTCTTC TCATCAACTA TCACCAACAT 
AATGGAGGAG GCCTGGTGAC TCGACTCCGG TATCCAGTTT GTTTTGGGAG GCAGAAAGCC 
CCAGTTACAG CAGGGCTGAG ATACGGGAAA TGGGTGATCG ACCCCTCAGA GCTCACTTTT 
GTGCAAGAGA TTGGCAGTGG GCAATTTGGG TTGGTGCATC TGGGCTACTG GCTCAACAAG 
GACAAGGTGG CTATCAAAAC CATTCGGGAA GGGGCTATGT CAGAAGAGGA CTTCATAGAG 
GAGGCTGAAG TAATGATGAA ACTCTCTCAT CCCAAACTGG TGCAGCTGTA TGGGGTGTGC 
CTGGAGCAGG CCCCCATCTG CCTGGTGTTT GAGTTCATGG AGCACGGCTG CCTGTCAGAT 
TATCTACGCA CCCAGCGGGG ACTTTTTGCT GCAGAGACCC TGCTGGGCAT GTGTCTGGAT 
GTGTGTGAGG GCATGGCCTA CCTGGAAGAG GCATGTGTCA TCCACAGAGA CTTGGCTGCC 
AGAAATTGTT TGGTGGGAGA AAACCAAGTC ATCAAGGTGT CTGACTTTGG GATGACAAGG 
TTCGTTCTGG ATGATCAGTA CACCAGTTCC ACAGGCACCA AATTCCCGGT GAAGTGGGCA 
TCCCCAGAGG TTTTCTCTTT CAGTCGCTAT AGCAGCAAGT CCGATGTGTG GTCATTTGGT 
GTGCTGATGT GGGAAGTTTT CAGTGAAGGC AAAATCCCGT ATGAAAACCG AAGCAACTCA 
GAGGTGGTGG AAGACATCAG TACCGGATTT CGGTTGTACA AGCCCCGGCT GGCCTCCACA 
CACGTCTACC AGATTATGAA TCACTGCTGG AAAGAGAGAC CAGAAGATCG GCCAGCCTTC 
TCCAGACTGC TGCGTCAACT GGCTGAAATT GCAGAATCAG GACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864932.1
CDS	71..1558
Translation	MDGKWRCCSQLEKLATGCAQYDPTKNASKKPLPPTPEDNRRPLWEPEETVVIALYDYQTNDPQELALRRNEEYCLLDSSEIHWWRVQDRNGHEGYVPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTAGTYTVSVFTKAVVSENNPCIKHYHIKETNDNPKRYYVAEKYVFDSIPLLINYHQHNGGGLVTRLRYPVCFGRQKAPVTAGLRYGKWVIDPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIREGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRTQRGLFAAETLLGMCLDVCEGMAYLEEACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASTHVYQIMNHCWKERPEDRPAFSRLLRQLAEIAESGL

Target ORF information:

RefSeq Version	XM_017009443.1
Organism	Homo sapiens(human)
Definition	Homo sapiens IL2 inducible T cell kinase (ITK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009443.1

ORF Insert Sequence:

ATGGATGGGA AGTGGAGGTG CTGTTCTCAG CTGGAGAAGC TTGCAACAGG CTGTGCCCAA 
TATGATCCAA CCAAGAATGC TTCAAAGAAG CCTCTTCCTC CTACTCCTGA AGACAACAGG 
CGACCACTTT GGGAACCTGA AGAAACTGTG GTCATTGCCT TATATGACTA CCAAACCAAT 
GATCCTCAGG AACTCGCACT GCGGCGCAAC GAAGAGTACT GCCTGCTGGA CAGTTCTGAG 
ATTCACTGGT GGAGAGTCCA GGACAGGAAT GGGCATGAAG GATATGTACC AAGCAGTTAT 
CTGGTGGAAA AATCTCCAAA TAATCTGGAA ACCTATGAGT GGTACAATAA GAGTATCAGC 
CGAGACAAAG CTGAAAAACT TCTTTTGGAC ACAGGCAAAG AAGGAGCCTT CATGGTAAGG 
GATTCCAGGA CTGCAGGAAC ATACACCGTG TCTGTTTTCA CCAAGGCTGT TGTAAGTGAG 
AACAATCCCT GTATAAAGCA TTATCACATC AAGGAAACAA ATGACAATCC TAAGCGATAC 
TATGTGGCTG AAAAGTATGT GTTCGATTCC ATCCCTCTTC TCATCAACTA TCACCAACAT 
AATGGAGGAG GCCTGGTGAC TCGACTCCGG TATCCAGTTT GTTTTGGGAG GCAGAAAGCC 
CCAGTTACAG CAGGGCTGAG ATACGGGAAA TGGGTGATCG ACCCCTCAGA GCTCACTTTT 
GTGCAAGAGA TTGGCAGTGG GCAATTTGGG TTGGTGCATC TGGGCTACTG GCTCAACAAG 
GACAAGGTGG CTATCAAAAC CATTCGGGAA GGGGCTATGT CAGAAGAGGA CTTCATAGAG 
GAGGCTGAAG TAATGATGAA ACTCTCTCAT CCCAAACTGG TGCAGCTGTA TGGGGTGTGC 
CTGGAGCAGG CCCCCATCTG CCTGGTGTTT GAGTTCATGG AGCACGGCTG CCTGTCAGAT 
TATCTACGCA CCCAGCGGGG ACTTTTTGCT GCAGAGACCC TGCTGGGCAT GTGTCTGGAT 
GTGTGTGAGG GCATGGCCTA CCTGGAAGAG GCATGTGTCA TCCACAGAGA CTTGGCTGCC 
AGAAATTGTT TGGTGGGAGA AAACCAAGTC ATCAAGGTGT CTGACTTTGG GATGACAAGG 
TTCGTTCTGG ATGATCAGTA CACCAGTTCC ACAGGCACCA AATTCCCGGT GAAGTGGGCA 
TCCCCAGAGG TTTTCTCTTT CAGTCGCTAT AGCAGCAAGT CCGATGTGTG GTCATTTGGT 
GTGCTGATGT GGGAAGTTTT CAGTGAAGGC AAAATCCCGT ATGAAAACCG AAGCAACTCA 
GAGGTGGTGG AAGACATCAG TACCGGATTT CGGTTGTACA AGCCCCGGCT GGCCTCCACA 
CACGTCTACC AGATTATGAA TCACTGCTGG AAAGAGAGAC CAGAAGATCG GCCAGCCTTC 
TCCAGACTGC TGCGTCAACT GGCTGAAATT GCAGAATCAG GACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITK ( NM_005546.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005537.3 Homo sapiens IL2 inducible T cell kinase (ITK), mRNA.

Price & Availability↑

CloneID	OHu18356
Clone ID Related Accession (Same CDS sequence)	NM_005546.3 , NM_005546.4
Accession Version	NM_005546.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	tyrosine-protein kinase ITK/TSK
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB150488.1, D13720.1, AC010609.7 and BX538196.1. On Sep 27, 2019 this sequence version replaced NM_005546.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D13720.1, BC109078.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAACAACT TTATCCTCCT GGAAGAACAG CTCATCAAGA AATCCCAACA AAAGAGAAGA 
ACTTCTCCCT CGAACTTTAA AGTCCGCTTC TTTGTGTTAA CCAAAGCCAG CCTGGCATAC 
TTTGAAGATC GTCATGGGAA GAAGCGCACG CTGAAGGGGT CCATTGAGCT CTCCCGAATC 
AAATGTGTTG AGATTGTGAA AAGTGACATC AGCATCCCAT GCCACTATAA ATACCCGTTT 
CAGGTGGTGC ATGACAACTA CCTCCTATAT GTGTTTGCTC CAGATCGTGA GAGCCGGCAG 
CGCTGGGTGC TGGCCCTTAA AGAAGAAACG AGGAATAATA ACAGTTTGGT GCCTAAATAT 
CATCCTAATT TCTGGATGGA TGGGAAGTGG AGGTGCTGTT CTCAGCTGGA GAAGCTTGCA 
ACAGGCTGTG CCCAATATGA TCCAACCAAG AATGCTTCAA AGAAGCCTCT TCCTCCTACT 
CCTGAAGACA ACAGGCGACC ACTTTGGGAA CCTGAAGAAA CTGTGGTCAT TGCCTTATAT 
GACTACCAAA CCAATGATCC TCAGGAACTC GCACTGCGGC GCAACGAAGA GTACTGCCTG 
CTGGACAGTT CTGAGATTCA CTGGTGGAGA GTCCAGGACA GGAATGGGCA TGAAGGATAT 
GTACCAAGCA GTTATCTGGT GGAAAAATCT CCAAATAATC TGGAAACCTA TGAGTGGTAC 
AATAAGAGTA TCAGCCGAGA CAAAGCTGAA AAACTTCTTT TGGACACAGG CAAAGAAGGA 
GCCTTCATGG TAAGGGATTC CAGGACTGCA GGAACATACA CCGTGTCTGT TTTCACCAAG 
GCTGTTGTAA GTGAGAACAA TCCCTGTATA AAGCATTATC ACATCAAGGA AACAAATGAC 
AATCCTAAGC GATACTATGT GGCTGAAAAG TATGTGTTCG ATTCCATCCC TCTTCTCATC 
AACTATCACC AACATAATGG AGGAGGCCTG GTGACTCGAC TCCGGTATCC AGTTTGTTTT 
GGGAGGCAGA AAGCCCCAGT TACAGCAGGG CTGAGATACG GGAAATGGGT GATCGACCCC 
TCAGAGCTCA CTTTTGTGCA AGAGATTGGC AGTGGGCAAT TTGGGTTGGT GCATCTGGGC 
TACTGGCTCA ACAAGGACAA GGTGGCTATC AAAACCATTC GGGAAGGGGC TATGTCAGAA 
GAGGACTTCA TAGAGGAGGC TGAAGTAATG ATGAAACTCT CTCATCCCAA ACTGGTGCAG 
CTGTATGGGG TGTGCCTGGA GCAGGCCCCC ATCTGCCTGG TGTTTGAGTT CATGGAGCAC 
GGCTGCCTGT CAGATTATCT ACGCACCCAG CGGGGACTTT TTGCTGCAGA GACCCTGCTG 
GGCATGTGTC TGGATGTGTG TGAGGGCATG GCCTACCTGG AAGAGGCATG TGTCATCCAC 
AGAGACTTGG CTGCCAGAAA TTGTTTGGTG GGAGAAAACC AAGTCATCAA GGTGTCTGAC 
TTTGGGATGA CAAGGTTCGT TCTGGATGAT CAGTACACCA GTTCCACAGG CACCAAATTC 
CCGGTGAAGT GGGCATCCCC AGAGGTTTTC TCTTTCAGTC GCTATAGCAG CAAGTCCGAT 
GTGTGGTCAT TTGGTGTGCT GATGTGGGAA GTTTTCAGTG AAGGCAAAAT CCCGTATGAA 
AACCGAAGCA ACTCAGAGGT GGTGGAAGAC ATCAGTACCG GATTTCGGTT GTACAAGCCC 
CGGCTGGCCT CCACACACGT CTACCAGATT ATGAATCACT GCTGGAAAGA GAGACCAGAA 
GATCGGCCAG CCTTCTCCAG ACTGCTGCGT CAACTGGCTG AAATTGCAGA ATCAGGACTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005537.3
CDS	139..2001
Translation	MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSDISIPCHYKYPFQVVHDNYLLYVFAPDRESRQRWVLALKEETRNNNSLVPKYHPNFWMDGKWRCCSQLEKLATGCAQYDPTKNASKKPLPPTPEDNRRPLWEPEETVVIALYDYQTNDPQELALRRNEEYCLLDSSEIHWWRVQDRNGHEGYVPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTAGTYTVSVFTKAVVSENNPCIKHYHIKETNDNPKRYYVAEKYVFDSIPLLINYHQHNGGGLVTRLRYPVCFGRQKAPVTAGLRYGKWVIDPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIREGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRTQRGLFAAETLLGMCLDVCEGMAYLEEACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASTHVYQIMNHCWKERPEDRPAFSRLLRQLAEIAESGL

Target ORF information:

RefSeq Version	NM_005546.4
Organism	Homo sapiens(human)
Definition	Homo sapiens IL2 inducible T cell kinase (ITK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005546.4

ORF Insert Sequence:

ATGAACAACT TTATCCTCCT GGAAGAACAG CTCATCAAGA AATCCCAACA AAAGAGAAGA 
ACTTCTCCCT CGAACTTTAA AGTCCGCTTC TTTGTGTTAA CCAAAGCCAG CCTGGCATAC 
TTTGAAGATC GTCATGGGAA GAAGCGCACG CTGAAGGGGT CCATTGAGCT CTCCCGAATC 
AAATGTGTTG AGATTGTGAA AAGTGACATC AGCATCCCAT GCCACTATAA ATACCCGTTT 
CAGGTGGTGC ATGACAACTA CCTCCTATAT GTGTTTGCTC CAGATCGTGA GAGCCGGCAG 
CGCTGGGTGC TGGCCCTTAA AGAAGAAACG AGGAATAATA ACAGTTTGGT GCCTAAATAT 
CATCCTAATT TCTGGATGGA TGGGAAGTGG AGGTGCTGTT CTCAGCTGGA GAAGCTTGCA 
ACAGGCTGTG CCCAATATGA TCCAACCAAG AATGCTTCAA AGAAGCCTCT TCCTCCTACT 
CCTGAAGACA ACAGGCGACC ACTTTGGGAA CCTGAAGAAA CTGTGGTCAT TGCCTTATAT 
GACTACCAAA CCAATGATCC TCAGGAACTC GCACTGCGGC GCAACGAAGA GTACTGCCTG 
CTGGACAGTT CTGAGATTCA CTGGTGGAGA GTCCAGGACA GGAATGGGCA TGAAGGATAT 
GTACCAAGCA GTTATCTGGT GGAAAAATCT CCAAATAATC TGGAAACCTA TGAGTGGTAC 
AATAAGAGTA TCAGCCGAGA CAAAGCTGAA AAACTTCTTT TGGACACAGG CAAAGAAGGA 
GCCTTCATGG TAAGGGATTC CAGGACTGCA GGAACATACA CCGTGTCTGT TTTCACCAAG 
GCTGTTGTAA GTGAGAACAA TCCCTGTATA AAGCATTATC ACATCAAGGA AACAAATGAC 
AATCCTAAGC GATACTATGT GGCTGAAAAG TATGTGTTCG ATTCCATCCC TCTTCTCATC 
AACTATCACC AACATAATGG AGGAGGCCTG GTGACTCGAC TCCGGTATCC AGTTTGTTTT 
GGGAGGCAGA AAGCCCCAGT TACAGCAGGG CTGAGATACG GGAAATGGGT GATCGACCCC 
TCAGAGCTCA CTTTTGTGCA AGAGATTGGC AGTGGGCAAT TTGGGTTGGT GCATCTGGGC 
TACTGGCTCA ACAAGGACAA GGTGGCTATC AAAACCATTC GGGAAGGGGC TATGTCAGAA 
GAGGACTTCA TAGAGGAGGC TGAAGTAATG ATGAAACTCT CTCATCCCAA ACTGGTGCAG 
CTGTATGGGG TGTGCCTGGA GCAGGCCCCC ATCTGCCTGG TGTTTGAGTT CATGGAGCAC 
GGCTGCCTGT CAGATTATCT ACGCACCCAG CGGGGACTTT TTGCTGCAGA GACCCTGCTG 
GGCATGTGTC TGGATGTGTG TGAGGGCATG GCCTACCTGG AAGAGGCATG TGTCATCCAC 
AGAGACTTGG CTGCCAGAAA TTGTTTGGTG GGAGAAAACC AAGTCATCAA GGTGTCTGAC 
TTTGGGATGA CAAGGTTCGT TCTGGATGAT CAGTACACCA GTTCCACAGG CACCAAATTC 
CCGGTGAAGT GGGCATCCCC AGAGGTTTTC TCTTTCAGTC GCTATAGCAG CAAGTCCGAT 
GTGTGGTCAT TTGGTGTGCT GATGTGGGAA GTTTTCAGTG AAGGCAAAAT CCCGTATGAA 
AACCGAAGCA ACTCAGAGGT GGTGGAAGAC ATCAGTACCG GATTTCGGTT GTACAAGCCC 
CGGCTGGCCT CCACACACGT CTACCAGATT ATGAATCACT GCTGGAAAGA GAGACCAGAA 
GATCGGCCAG CCTTCTCCAG ACTGCTGCGT CAACTGGCTG AAATTGCAGA ATCAGGACTT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ITK signalling via the Ras/IRF4 pathway regulates the development and function of Tr1 cells.
Nature communications815871(2017 Jun)
Huang W,Solouki S,Koylass N,Zheng SG,August A

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Inducible T Cell Kinase Regulates the Acquisition of Cytolytic Capacity and Degranulation in CD8
Journal of immunology (Baltimore, Md. : 1950)198(7)2699-2711(2017 Apr)
Kapnick SM,Stinchcombe JC,Griffiths GM,Schwartzberg PL

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ITK cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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