S100A7A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	S100A7A
Entrez Gene ID	338324
Full Name	S100 calcium binding protein A7A
Synonyms	NICE-2,NICE2,S100A15,S100A7L1,S100A7f
General protein information	Preferred Names S100 calcium binding protein A7A Names protein S100-A7A S100 calcium-binding protein A15 S100 calcium-binding protein A7-like 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q21.3
Disorder MIM:	617427

mRNA and Protein(s)

mRNA	Protein	Name
NM_176823.4	NP_789793.1	protein S100-A7A
NM_176823.3	NP_789793.1	protein S100-A7A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Structural characterization of S100A15 reveals a novel zinc coordination site among S100 proteins and altered surface chemistry with functional implications for receptor binding.
Murray JI, Tonkin ML, Whiting AL, Peng F, Farnell B, Cullen JT, Hof F, Boulanger MJ
BMC structural biology1216(2012 Jul)

Gene from a psoriasis susceptibility locus primes the skin for inflammation.
Wolf R, Mascia F, Dharamsi A, Howard OM, Cataisson C, Bliskovski V, Winston J, Feigenbaum L, Lichti U, Ruzicka T, Chavakis T, Yuspa SH
Science translational medicine2(61)61ra90(2010 Dec)

Chemotactic activity of S100A7 (Psoriasin) is mediated by the receptor for advanced glycation end products and potentiates inflammation with highly homologous but functionally distinct S100A15.
Wolf R, Howard OM, Dong HF, Voscopoulos C, Boeshans K, Winston J, Divi R, Gunsior M, Goldsmith P, Ahvazi B, Chavakis T, Oppenheim JJ, Yuspa SH
Journal of immunology (Baltimore, Md. : 1950)181(2)1499-506(2008 Jul)

S100A15, an antimicrobial protein of the skin: regulation by E. coli through Toll-like receptor 4.
B?chau AS, Hassan M, Kukova G, Lewerenz V, Kellermann S, W?rthner JU, Wolf R, Walz M, Gallo RL, Ruzicka T
The Journal of investigative dermatology127(11)2596-604(2007 Nov)

Human S100A15 splice variants are differentially expressed in inflammatory skin diseases and regulated through Th1 cytokines and calcium.
Wolf R, Lewerenz V, B?chau AS, Walz M, Ruzicka T
Experimental dermatology16(8)685-91(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

S100A15 retains the ability to coordinate zinc through incorporation of an exogenous ligand resulting in a unique zinc-binding site among S100 proteins.
Title: Structural characterization of S100A15 reveals a novel zinc coordination site among S100 proteins and altered surface chemistry with functional implications for receptor binding.

study defines RAGE (receptor for advanced glycation end products) as the hS100A7 receptor, whereas hS100A15 functions through a Gi protein-coupled receptor
Title: Chemotactic activity of S100A7 (Psoriasin) is mediated by the receptor for advanced glycation end products and potentiates inflammation with highly homologous but functionally distinct S100A15.

E. coli modulates S100A15 and IL-8 expression of keratinocytes by recognition through TLR4.
Title: S100A15, an antimicrobial protein of the skin: regulation by E. coli through Toll-like receptor 4.

Overexpression of hS100A15 in chronic inflammatory skin diseases and regulation by inflammatory cytokines and calcium suggest that hS100A15 is involved in Th1-associated epithelial responses and epidermal maturation in normal and diseased human skin
Title: Human S100A15 splice variants are differentially expressed in inflammatory skin diseases and regulated through Th1 cytokines and calcium.

The S100A15 protein has been expressed & purified to homogeneity in 2 crystal forms. Crystals of form I are triclinic. Crystals of form II are monoclinic & have a solvent content of 51.8%, corresponding to the presence of 1 monomer in the asymmetric unit.
Title: Purification, crystallization and preliminary X-ray diffraction of human S100A15.

The following S100A7A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the S100A7A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30121	NM_176823.4 Latest version!	Homo sapiens S100 calcium binding protein A7A (S100A7A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu30121	NM_176823.3	Homo sapiens S100 calcium binding protein A7A (S100A7A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

S100A7A ( NM_176823.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_789793.1 Homo sapiens S100 calcium binding protein A7A (S100A7A), mRNA.

Price & Availability↑

CloneID	OHu30121
Clone ID Related Accession (Same CDS sequence)	NM_176823.4 , NM_176823.3
Accession Version	NM_176823.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	protein S100-A7A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL591704.7, AY189118.1 and AJ243672.2. On Nov 22, 2018 this sequence version replaced NM_176823.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY189118.1, AY189119.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMEA2147975 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000368729.9/ ENSP00000357718.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCAACA CTCAAGCTGA GAGGTCCATA ATAGGCATGA TCGACATGTT TCACAAATAC 
ACCGGACGTG ATGGCAAGAT TGAGAAGCCA AGCCTGCTGA CGATGATGAA GGAGAACTTC 
CCCAATTTCC TCAGTGCCTG TGACAAAAAG GGCATACATT ACCTCGCCAC TGTCTTTGAG 
AAAAAGGACA AGAATGAGGA TAAGAAGATT GATTTTTCTG AGTTTCTGTC CTTGCTGGGA 
GACATAGCCG CAGACTACCA CAAGCAGAGC CATGGAGCGG CGCCCTGTTC TGGGGGAAGC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_789793.1
CDS	62..367
Translation	MSNTQAERSIIGMIDMFHKYTGRDGKIEKPSLLTMMKENFPNFLSACDKKGIHYLATVFEKKDKNEDKKIDFSEFLSLLGDIAADYHKQSHGAAPCSGGSQ

Target ORF information:

RefSeq Version	NM_176823.4
Organism	Homo sapiens(human)
Definition	Homo sapiens S100 calcium binding protein A7A (S100A7A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176823.4

ORF Insert Sequence:

ATGAGCAACA CTCAAGCTGA GAGGTCCATA ATAGGCATGA TCGACATGTT TCACAAATAC 
ACCGGACGTG ATGGCAAGAT TGAGAAGCCA AGCCTGCTGA CGATGATGAA GGAGAACTTC 
CCCAATTTCC TCAGTGCCTG TGACAAAAAG GGCATACATT ACCTCGCCAC TGTCTTTGAG 
AAAAAGGACA AGAATGAGGA TAAGAAGATT GATTTTTCTG AGTTTCTGTC CTTGCTGGGA 
GACATAGCCG CAGACTACCA CAAGCAGAGC CATGGAGCGG CGCCCTGTTC TGGGGGAAGC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

S100A7A ( NM_176823.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_789793.1 Homo sapiens S100 calcium binding protein A7A (S100A7A), mRNA.

Price & Availability↑

CloneID	OHu30121
Clone ID Related Accession (Same CDS sequence)	NM_176823.4 , NM_176823.3
Accession Version	NM_176823.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	protein S100-A7A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY189118.1 and AJ243672.2. On Feb 23, 2007 this sequence version replaced NM_176823.2. ##Evidence-Data-START## Transcript exon combination :: AY189118.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMEA2147975 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCAACA CTCAAGCTGA GAGGTCCATA ATAGGCATGA TCGACATGTT TCACAAATAC 
ACCGGACGTG ATGGCAAGAT TGAGAAGCCA AGCCTGCTGA CGATGATGAA GGAGAACTTC 
CCCAATTTCC TCAGTGCCTG TGACAAAAAG GGCATACATT ACCTCGCCAC TGTCTTTGAG 
AAAAAGGACA AGAATGAGGA TAAGAAGATT GATTTTTCTG AGTTTCTGTC CTTGCTGGGA 
GACATAGCCG CAGACTACCA CAAGCAGAGC CATGGAGCGG CGCCCTGTTC TGGGGGAAGC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_789793.1
CDS	58..363
Translation	MSNTQAERSIIGMIDMFHKYTGRDGKIEKPSLLTMMKENFPNFLSACDKKGIHYLATVFEKKDKNEDKKIDFSEFLSLLGDIAADYHKQSHGAAPCSGGSQ

Target ORF information:

RefSeq Version	NM_176823.3
Organism	Homo sapiens(human)
Definition	Homo sapiens S100 calcium binding protein A7A (S100A7A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176823.3

ORF Insert Sequence:

ATGAGCAACA CTCAAGCTGA GAGGTCCATA ATAGGCATGA TCGACATGTT TCACAAATAC 
ACCGGACGTG ATGGCAAGAT TGAGAAGCCA AGCCTGCTGA CGATGATGAA GGAGAACTTC 
CCCAATTTCC TCAGTGCCTG TGACAAAAAG GGCATACATT ACCTCGCCAC TGTCTTTGAG 
AAAAAGGACA AGAATGAGGA TAAGAAGATT GATTTTTCTG AGTTTCTGTC CTTGCTGGGA 
GACATAGCCG CAGACTACCA CAAGCAGAGC CATGGAGCGG CGCCCTGTTC TGGGGGAAGC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural characterization of S100A15 reveals a novel zinc coordination site among S100 proteins and altered surface chemistry with functional implications for receptor binding.
BMC structural biology1216(2012 Jul)
Murray JI,Tonkin ML,Whiting AL,Peng F,Farnell B,Cullen JT,Hof F,Boulanger MJ

Gene from a psoriasis susceptibility locus primes the skin for inflammation.
Science translational medicine2(61)61ra90(2010 Dec)
Wolf R,Mascia F,Dharamsi A,Howard OM,Cataisson C,Bliskovski V,Winston J,Feigenbaum L,Lichti U,Ruzicka T,Chavakis T,Yuspa SH

Chemotactic activity of S100A7 (Psoriasin) is mediated by the receptor for advanced glycation end products and potentiates inflammation with highly homologous but functionally distinct S100A15.
Journal of immunology (Baltimore, Md. : 1950)181(2)1499-506(2008 Jul)
Wolf R,Howard OM,Dong HF,Voscopoulos C,Boeshans K,Winston J,Divi R,Gunsior M,Goldsmith P,Ahvazi B,Chavakis T,Oppenheim JJ,Yuspa SH

S100A15, an antimicrobial protein of the skin: regulation by E. coli through Toll-like receptor 4.
The Journal of investigative dermatology127(11)2596-604(2007 Nov)
B?chau AS,Hassan M,Kukova G,Lewerenz V,Kellermann S,W?rthner JU,Wolf R,Walz M,Gallo RL,Ruzicka T

Human S100A15 splice variants are differentially expressed in inflammatory skin diseases and regulated through Th1 cytokines and calcium.
Experimental dermatology16(8)685-91(2007 Aug)
Wolf R,Lewerenz V,B?chau AS,Walz M,Ruzicka T