TLX2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLX2
Entrez Gene ID	3196
Full Name	T cell leukemia homeobox 2
Synonyms	HOX11L1,NCX
General protein information	Preferred Names T cell leukemia homeobox 2 Names T-cell leukemia homeobox protein 2 homeo box 11-like 1 homeobox protein Hox-11L1 neural crest homeobox protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p13.1
Summary	This gene is a member of an orphan homeobox-containing transcription factor family. Studies of the mouse ortholog have shown that the encoded protein is crucial for the development of the enteric nervous system; in humans, loss-of-function may play a role in tumorigenesis of gastrointestinal stromal tumors. [provided by RefSeq, May 2010].
Disorder MIM:	604240

mRNA and Protein(s)

mRNA	Protein	Name
NM_016170.4	NP_057254.1	T-cell leukemia homeobox protein 2
NM_016170.5	NP_057254.1	T-cell leukemia homeobox protein 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TLX2	XP_855075.1
Bos taurus (cattle)	TLX2	NP_001179087.1
Gallus gallus (chicken)	TLX2	XP_004949910.1
	TLX2	XP_001110250.1
Rattus norvegicus (Norway rat)	Tlx2	NP_001165596.1
Homo sapiens (human)	TLX2	NP_057254.1
Mus musculus (house mouse)	Tlx2	NP_033418.1

Gene Ontology
Bibliography

Related articles in PubMed

Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.
Oshikawa M, Tsutsui C, Ikegami T, Fuchida Y, Matsubara M, Toyama S, Usami R, Ohtoko K, Kato S
Investigative ophthalmology & visual science52(9)6662-70(2011 Aug)

Selective interference with TRPC3/6 channels disrupts OX1 receptor signalling via NCX and reveals a distinct calcium influx pathway.
Louhivuori LM, Jansson L, Nordstr?m T, Bart G, N?sman J, Akerman KE
Cell calcium48(2-3)114-23(2010 Aug-Sep)

Na+/Ca2+ exchanger is a determinant of excitation-contraction coupling in human embryonic stem cell-derived ventricular cardiomyocytes.
Fu JD, Jiang P, Rushing S, Liu J, Chiamvimonvat N, Li RA
Stem cells and development19(6)773-82(2010 Jun)

Functional characterization of a minimal sequence essential for the expression of human TLX2 gene.
Borghini S, Bachetti T, Fava M, Duca MD, Ravazzolo R, Ceccherini I
BMB reports42(12)788-93(2009 Dec)

A census of human transcription factors: function, expression and evolution.
Vaquerizas JM, Kummerfeld SK, Teichmann SA, Luscombe NM
Nature reviews. Genetics10(4)252-63(2009 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NCX-TRPC channel interaction constitutes an important functional unit in receptor-mediated divalent calcium ion influx in neuronal cells.
Title: Selective interference with TRPC3/6 channels disrupts OX1 receptor signalling via NCX and reveals a distinct calcium influx pathway.

NCX is functionally expressed in developing ventricular human embryonic stem cell-derived ventricular cardiomyocytes and contributes to their excitation-contraction coupling.
Title: Na+/Ca2+ exchanger is a determinant of excitation-contraction coupling in human embryonic stem cell-derived ventricular cardiomyocytes.

TLX2 is an orphan homeodomain transcription factor whose expression is mainly associated with tissues derived from neural crest cells.
Title: Functional characterization of a minimal sequence essential for the expression of human TLX2 gene.

Hox11L1 has a role in tumorigenesis in gastrointestinal stromal tumors
Title: Allelic loss of Hox11L1 gene locus predicts outcome of gastrointestinal stromal tumors.

These results support the PHOX2B-TLX2 promoter interaction, suggesting a physiological role in the transcription-factor cascade underlying the differentiation of neuronal lineages of the Autonomic Nervous System during human embryogenesis.
Title: The TLX2 homeobox gene is a transcriptional target of PHOX2B in neural-crest-derived cells.

The following TLX2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLX2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08531	NM_016170.5 Latest version!	Homo sapiens T cell leukemia homeobox 2 (TLX2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu08531	NM_016170.4	Homo sapiens T cell leukemia homeobox 2 (TLX2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLX2 ( NM_016170.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057254.1 Homo sapiens T cell leukemia homeobox 2 (TLX2), mRNA.

Price & Availability↑

CloneID	OHu08531
Clone ID Related Accession (Same CDS sequence)	NM_016170.4 , NM_016170.5
Accession Version	NM_016170.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Homo sapiens(human)
Product	T-cell leukemia homeobox protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005041.2, BC006356.2 and BU740503.1. On May 6, 2010 this sequence version replaced NM_016170.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006356.2, AB008501.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGCCGG GGATGCTGGG TCCACACAAC CTCCCACACC ACGAGCCAAT CAGCTTCGGC 
ATCGATCAGA TCCTGAGCGG CCCCGAAACC CCAGGGGGCG GTCTAGGCCT GGGTCGCGGG 
GGCCAGGGTC ATGGGGAGAA TGGGGCGTTC TCGGGTGGAT ACCACGGAGC CTCGGGCTAC 
GGTCCCGCCG GCTCACTTGC CCCGCTGCCC GGCAGCTCCG GAGTGGGCCC AGGCGGCGTG 
ATCCGCGTCC CTGCGCACCG CCCGCTGCCT GTGCCGCCGC CCGCTGGGGG GGCGCCTGCA 
GTGCCTGGGC CCTCGGGTTT GGGCGGCGCC GGAGGCCTAG CGGGACTCAC CTTCCCCTGG 
ATGGACAGCG GCCGCCGCTT TGCCAAGGAC CGGCTCACGG CTGCGCTCTC GCCCTTCTCT 
GGGACGCGCC GCATAGGCCA CCCCTACCAA AACCGGACCC CTCCGAAGCG GAAGAAGCCG 
CGCACGTCCT TCTCCCGCTC ACAGGTGCTG GAGTTGGAGC GGCGCTTCCT GCGCCAGAAG 
TACCTGGCCT CTGCGGAGAG GGCGGCGCTG GCCAAGGCCT TGCGCATGAC CGACGCACAG 
GTCAAAACGT GGTTCCAGAA CCGACGCACC AAGTGGCGGC GCCAGACGGC GGAGGAGCGC 
GAGGCCGAGC GGCACCGCGC GGGCCGGCTG CTCCTGCATC TGCAGCAGGA CGCGTTGCCA 
CGGCCGCTGC GGCCGCCGCT GCCCCCGGAC CCTCTCTGCC TGCACAACTC GTCGCTCTTC 
GCGCTGCAGA ACCTGCAGCC CTGGGCCGAG GACAACAAAG TGGCTTCAGT GTCCGGGCTC 
GCCTCGGTGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057254.1
CDS	339..1193
Translation	MEPGMLGPHNLPHHEPISFGIDQILSGPETPGGGLGLGRGGQGHGENGAFSGGYHGASGYGPAGSLAPLPGSSGVGPGGVIRVPAHRPLPVPPPAGGAPAVPGPSGLGGAGGLAGLTFPWMDSGRRFAKDRLTAALSPFSGTRRIGHPYQNRTPPKRKKPRTSFSRSQVLELERRFLRQKYLASAERAALAKALRMTDAQVKTWFQNRRTKWRRQTAEEREAERHRAGRLLLHLQQDALPRPLRPPLPPDPLCLHNSSLFALQNLQPWAEDNKVASVSGLASVV

Target ORF information:

RefSeq Version	NM_016170.4
Organism	Homo sapiens(human)
Definition	Homo sapiens T cell leukemia homeobox 2 (TLX2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016170.4

ORF Insert Sequence:

ATGGAGCCGG GGATGCTGGG TCCACACAAC CTCCCACACC ACGAGCCAAT CAGCTTCGGC 
ATCGATCAGA TCCTGAGCGG CCCCGAAACC CCAGGGGGCG GTCTAGGCCT GGGTCGCGGG 
GGCCAGGGTC ATGGGGAGAA TGGGGCGTTC TCGGGTGGAT ACCACGGAGC CTCGGGCTAC 
GGTCCCGCCG GCTCACTTGC CCCGCTGCCC GGCAGCTCCG GAGTGGGCCC AGGCGGCGTG 
ATCCGCGTCC CTGCGCACCG CCCGCTGCCT GTGCCGCCGC CCGCTGGGGG GGCGCCTGCA 
GTGCCTGGGC CCTCGGGTTT GGGCGGCGCC GGAGGCCTAG CGGGACTCAC CTTCCCCTGG 
ATGGACAGCG GCCGCCGCTT TGCCAAGGAC CGGCTCACGG CTGCGCTCTC GCCCTTCTCT 
GGGACGCGCC GCATAGGCCA CCCCTACCAA AACCGGACCC CTCCGAAGCG GAAGAAGCCG 
CGCACGTCCT TCTCCCGCTC ACAGGTGCTG GAGTTGGAGC GGCGCTTCCT GCGCCAGAAG 
TACCTGGCCT CTGCGGAGAG GGCGGCGCTG GCCAAGGCCT TGCGCATGAC CGACGCACAG 
GTCAAAACGT GGTTCCAGAA CCGACGCACC AAGTGGCGGC GCCAGACGGC GGAGGAGCGC 
GAGGCCGAGC GGCACCGCGC GGGCCGGCTG CTCCTGCATC TGCAGCAGGA CGCGTTGCCA 
CGGCCGCTGC GGCCGCCGCT GCCCCCGGAC CCTCTCTGCC TGCACAACTC GTCGCTCTTC 
GCGCTGCAGA ACCTGCAGCC CTGGGCCGAG GACAACAAAG TGGCTTCAGT GTCCGGGCTC 
GCCTCGGTGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLX2 ( NM_016170.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057254.1 Homo sapiens T cell leukemia homeobox 2 (TLX2), mRNA.

Price & Availability↑

CloneID	OHu08531
Clone ID Related Accession (Same CDS sequence)	NM_016170.4 , NM_016170.5
Accession Version	NM_016170.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	T-cell leukemia homeobox protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005041.2, BC006356.2 and BU740503.1. On Nov 24, 2018 this sequence version replaced NM_016170.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006356.2, AB008501.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000233638.8/ ENSP00000233638.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCCGG GGATGCTGGG TCCACACAAC CTCCCACACC ACGAGCCAAT CAGCTTCGGC 
ATCGATCAGA TCCTGAGCGG CCCCGAAACC CCAGGGGGCG GTCTAGGCCT GGGTCGCGGG 
GGCCAGGGTC ATGGGGAGAA TGGGGCGTTC TCGGGTGGAT ACCACGGAGC CTCGGGCTAC 
GGTCCCGCCG GCTCACTTGC CCCGCTGCCC GGCAGCTCCG GAGTGGGCCC AGGCGGCGTG 
ATCCGCGTCC CTGCGCACCG CCCGCTGCCT GTGCCGCCGC CCGCTGGGGG GGCGCCTGCA 
GTGCCTGGGC CCTCGGGTTT GGGCGGCGCC GGAGGCCTAG CGGGACTCAC CTTCCCCTGG 
ATGGACAGCG GCCGCCGCTT TGCCAAGGAC CGGCTCACGG CTGCGCTCTC GCCCTTCTCT 
GGGACGCGCC GCATAGGCCA CCCCTACCAA AACCGGACCC CTCCGAAGCG GAAGAAGCCG 
CGCACGTCCT TCTCCCGCTC ACAGGTGCTG GAGTTGGAGC GGCGCTTCCT GCGCCAGAAG 
TACCTGGCCT CTGCGGAGAG GGCGGCGCTG GCCAAGGCCT TGCGCATGAC CGACGCACAG 
GTCAAAACGT GGTTCCAGAA CCGACGCACC AAGTGGCGGC GCCAGACGGC GGAGGAGCGC 
GAGGCCGAGC GGCACCGCGC GGGCCGGCTG CTCCTGCATC TGCAGCAGGA CGCGTTGCCA 
CGGCCGCTGC GGCCGCCGCT GCCCCCGGAC CCTCTCTGCC TGCACAACTC GTCGCTCTTC 
GCGCTGCAGA ACCTGCAGCC CTGGGCCGAG GACAACAAAG TGGCTTCAGT GTCCGGGCTC 
GCCTCGGTGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057254.1
CDS	358..1212
Translation	MEPGMLGPHNLPHHEPISFGIDQILSGPETPGGGLGLGRGGQGHGENGAFSGGYHGASGYGPAGSLAPLPGSSGVGPGGVIRVPAHRPLPVPPPAGGAPAVPGPSGLGGAGGLAGLTFPWMDSGRRFAKDRLTAALSPFSGTRRIGHPYQNRTPPKRKKPRTSFSRSQVLELERRFLRQKYLASAERAALAKALRMTDAQVKTWFQNRRTKWRRQTAEEREAERHRAGRLLLHLQQDALPRPLRPPLPPDPLCLHNSSLFALQNLQPWAEDNKVASVSGLASVV

Target ORF information:

RefSeq Version	NM_016170.5
Organism	Homo sapiens(human)
Definition	Homo sapiens T cell leukemia homeobox 2 (TLX2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016170.5

ORF Insert Sequence:

ATGGAGCCGG GGATGCTGGG TCCACACAAC CTCCCACACC ACGAGCCAAT CAGCTTCGGC 
ATCGATCAGA TCCTGAGCGG CCCCGAAACC CCAGGGGGCG GTCTAGGCCT GGGTCGCGGG 
GGCCAGGGTC ATGGGGAGAA TGGGGCGTTC TCGGGTGGAT ACCACGGAGC CTCGGGCTAC 
GGTCCCGCCG GCTCACTTGC CCCGCTGCCC GGCAGCTCCG GAGTGGGCCC AGGCGGCGTG 
ATCCGCGTCC CTGCGCACCG CCCGCTGCCT GTGCCGCCGC CCGCTGGGGG GGCGCCTGCA 
GTGCCTGGGC CCTCGGGTTT GGGCGGCGCC GGAGGCCTAG CGGGACTCAC CTTCCCCTGG 
ATGGACAGCG GCCGCCGCTT TGCCAAGGAC CGGCTCACGG CTGCGCTCTC GCCCTTCTCT 
GGGACGCGCC GCATAGGCCA CCCCTACCAA AACCGGACCC CTCCGAAGCG GAAGAAGCCG 
CGCACGTCCT TCTCCCGCTC ACAGGTGCTG GAGTTGGAGC GGCGCTTCCT GCGCCAGAAG 
TACCTGGCCT CTGCGGAGAG GGCGGCGCTG GCCAAGGCCT TGCGCATGAC CGACGCACAG 
GTCAAAACGT GGTTCCAGAA CCGACGCACC AAGTGGCGGC GCCAGACGGC GGAGGAGCGC 
GAGGCCGAGC GGCACCGCGC GGGCCGGCTG CTCCTGCATC TGCAGCAGGA CGCGTTGCCA 
CGGCCGCTGC GGCCGCCGCT GCCCCCGGAC CCTCTCTGCC TGCACAACTC GTCGCTCTTC 
GCGCTGCAGA ACCTGCAGCC CTGGGCCGAG GACAACAAAG TGGCTTCAGT GTCCGGGCTC 
GCCTCGGTGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.
Investigative ophthalmology & visual science52(9)6662-70(2011 Aug)
Oshikawa M,Tsutsui C,Ikegami T,Fuchida Y,Matsubara M,Toyama S,Usami R,Ohtoko K,Kato S

Selective interference with TRPC3/6 channels disrupts OX1 receptor signalling via NCX and reveals a distinct calcium influx pathway.
Cell calcium48(2-3)114-23(2010 Aug-Sep)
Louhivuori LM,Jansson L,Nordstr?m T,Bart G,N?sman J,Akerman KE

Na+/Ca2+ exchanger is a determinant of excitation-contraction coupling in human embryonic stem cell-derived ventricular cardiomyocytes.
Stem cells and development19(6)773-82(2010 Jun)
Fu JD,Jiang P,Rushing S,Liu J,Chiamvimonvat N,Li RA

Functional characterization of a minimal sequence essential for the expression of human TLX2 gene.
BMB reports42(12)788-93(2009 Dec)
Borghini S,Bachetti T,Fava M,Duca MD,Ravazzolo R,Ceccherini I

A census of human transcription factors: function, expression and evolution.
Nature reviews. Genetics10(4)252-63(2009 04)
Vaquerizas JM,Kummerfeld SK,Teichmann SA,Luscombe NM