NCKAP1L cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NCKAP1L
Entrez Gene ID	3071
Full Name	NCK associated protein 1 like
Synonyms	HEM1
General protein information	Preferred Names NCK associated protein 1 like Names nck-associated protein 1-like hematopoietic protein 1 membrane-associated protein hem-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.13-q13.2
Summary	This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010].
Disorder MIM:	141180

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184976.2	NP_001171905.1	nck-associated protein 1-like isoform 2
NM_005337.5	NP_005328.2	nck-associated protein 1-like isoform 1
NM_005337.4	NP_005328.2	nck-associated protein 1-like isoform 1
NM_001184976.1	NP_001171905.1	nck-associated protein 1-like isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	NCKAP1L	NP_005328.2
Pan troglodytes (chimpanzee)	NCKAP1L	XP_509114.3
	NCKAP1L	XP_002798677.1
Bos taurus (cattle)	NCKAP1L	NP_001137348.1
Mus musculus (house mouse)	Nckap1l	NP_705725.1
Rattus norvegicus (Norway rat)	Nckap1l	NP_001101589.1
Danio rerio (zebrafish)	zgc:172352	NP_001107884.1
Gallus gallus (chicken)	NCKAP1L	NP_001026510.1
Xenopus tropicalis (tropical clawed frog)	nckap1l	XP_002935704.2
Canis lupus familiaris (dog)	NCKAP1L	XP_005636795.1

Gene Ontology
Bibliography

Related articles in PubMed

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
Comuzzie AG, Cole SA, Laston SL, Voruganti VS, Haack K, Gibbs RA, Butte NF
PloS one7(12)e51954(2012)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

ATM, CTLA4, MNDA, and HEM1 in high versus low CD38 expressing B-cell chronic lymphocytic leukemia.
Joshi AD, Hegde GV, Dickinson JD, Mittal AK, Lynch JC, Eudy JD, Armitage JO, Bierman PJ, Bociek RG, Devetten MP, Vose JM, Joshi SS
Clinical cancer research : an official journal of the American Association for Cancer Research13(18 Pt 1)5295-304(2007 Sep)

An actin-based wave generator organizes cell motility.
Weiner OD, Marganski WA, Wu LF, Altschuler SJ, Kirschner MW
PLoS biology5(9)e221(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the Hem-1/Nap1 component of the Scar/WAVE complex localizes to propagating waves that appear to organize the leading edge of a motile neutrophil
Title: An actin-based wave generator organizes cell motility.

versatile Hem-1-containing complexes coordinate diverse regulatory signals at the leading edge of polarized neutrophils, including but not confined to those involving Verprolin-homologous protein-dependent actin polymerization
Title: Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis.

The following NCKAP1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCKAP1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31086	NM_005337.5 Latest version!	Homo sapiens NCK associated protein 1 like (NCKAP1L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu55576	NM_001184976.2 Latest version!	Homo sapiens NCK associated protein 1 like (NCKAP1L), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
View Old Accession Versions >>
OHu31086	NM_005337.4	Homo sapiens NCK associated protein 1 like (NCKAP1L), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu55576	NM_001184976.1	Homo sapiens NCK associated protein 1 like (NCKAP1L), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCKAP1L ( NM_005337.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005328.2 Homo sapiens NCK associated protein 1 like (NCKAP1L), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31086
Clone ID Related Accession (Same CDS sequence)	NM_005337.5 , NM_005337.4
Accession Version	NM_005337.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	nck-associated protein 1-like isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA820115.1, BC093769.2, BC093771.1, M58285.1, AW575485.1 and AC068789.16. On Nov 23, 2018 this sequence version replaced NM_005337.4. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803613.269017.1, SRR1803617.36686.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000293373.11/ ENSP00000293373.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTTTGA CATCTGCTTA CCAGCATAAA TTAGCAGAGA AGCTCACTAT CCTGAATGAT 
CGCGGTCAGG GGGTTCTCAT CCGTATGTAT AACATCAAGA AGACTTGTTC AGACCCCAAA 
TCTAAGCCAC CTTTCTTACT GGAAAAGTCC ATGGAACCAT CTCTCAAGTA TATCAACAAG 
AAATTTCCCA ACATAGATGT CCGAAACAGC ACGCAACATT TAGGACCAGT ACATCGTGAA 
AAAGCCGAGA TAATTAGATT CCTCACCAAC TACTACCAGT CATTTGTGGA TGTCATGGAA 
TTTCGGGATC ATGTATATGA ACTTCTCAAC ACCATTGATG CCTGCCAGTG CCATTTTGAT 
ATCAATCTCA ACTTTGATTT CACTCGGAGT TACCTGGACT TGATTGTAAC TTACACCTCA 
GTCATTTTAC TTCTGTCACG GATTGAAGAT CGGCGGATAC TCATTGGCAT GTACAATTGT 
GCCCATGAGA TGCTGCATGG GCATGGTGAC CCCAGTTTTG CCCGTCTGGG TCAGATGGTC 
TTGGAGTATG ACCACCCTCT GAAGAAGCTG ACAGAAGAGT TTGGGCCTCA CACAAAGGCT 
GTGAGTGGAG CCCTCCTCTC TTTGCATTTC CTCTTTGTCC GAAGAAACCA GGGGGCTGAG 
CAGTGGCGCA GTGCCCAACT TCTAAGCCTC ATCAGCAACC CCCCAGCCAT GATTAACCCT 
GCTAATTCAG ATACAATGGC CTGTGAGTAT CTGTCTGTGG AAGTAATGGA GCGCTGGATT 
ATCATTGGGT TTCTTCTTTG TCATGGGTGC CTCAACTCCA ATAGCCAGTG CCAGAAGCTG 
TGGAAGCTGT GTCTGCAGGG CTCCCTCTAC ATCACCCTTA TCCGTGAGGA TGTGCTGCAG 
GTGCACAAAG TCACCGAGGA CCTGTTTAGC AGTTTGAAAG GGTATGGCAA GAGAGTGGCA 
GACATAAAGG AGAGCAAGGA ACATGTAATT GCAAACAGTG GCCAGTTTCA TTGTCAACGG 
CGGCAATTTC TGCGGATGGC AGTGAAGGAG CTGGAGACTG TGTTGGCTGA TGAACCGGGA 
CTACTGGGTC CTAAGGCTCT TTTTGCTTTC ATGGCCCTGT CCTTCATTCG TGATGAGGTC 
ACCTGGCTGG TTCGCCACAC AGAGAATGTC ACCAAGACAA AGACACCTGA GGACTATGCT 
GACTCGAGCA TTGCAGAGCT ACTTTTCTTG TTGGAGGGGA TTAGGTCTCT GGTCCGAAGA 
CACATCAAAG TGATACAGCA ATACCACCTT CAGTACTTGG CAAGATTTGA TGCTCTTGTG 
CTCAGTGACA TCATTCAGAA CTTGTCTGTG TGTCCAGAGG AGGAGTCCAT CATCATGTCC 
TCATTCGTCA GTATCCTCTC CTCTCTGAAT CTCAAACAAG TTGATAATGG AGAAAAATTT 
GAATTCTCAG GATTGAGGCT GGACTGGTTC CGCCTACAGG CATACACTAG CGTGGCTAAG 
GCCCCTCTGC ACCTGCATGA GAACCCTGAC TTAGCCAAGG TGATGAACCT CATTGTCTTC 
CACTCCCGAA TGCTGGACTC CGTAGAAAAA TTGCTGGTGG AAACTTCTGA TCTGTCTACT 
TTCTGCTTTC ATCTTCGTAT CTTTGAGAAG ATGTTTGCCA TGACCTTGGA GGAATCTGCC 
ATGTTGCGTT ATGCCATTGC TTTCCCCCTG ATTTGTGCTC ACTTTGTCCA CTGCACTCAT 
GAGATGTGCC CAGAGGAGTA CCCCCACCTC AAGAACCATG GTCTTCACCA CTGCAACTCC 
TTCCTGGAAG AGTTGGCCAA GCAGACCAGC AATTGCGTCC TGGAGATCTG TGCTGAGCAG 
CGAAACCTGA GCGAGCAGCT TCTACCTAAG CACTGTGCCA CTACAATCAG CAAAGCCAAG 
AACAAGAAAA CCAGGAAGCA GAGGCAGACT CCCAGAAAAG GAGAGCCCGA GAGGGACAAG 
CCAGGAGCTG AGAGTCACCG GAAGAACCGC AGCATTGTCA CCAACATGGA CAAGCTACAC 
CTAAACTTGA CAGAACTGGC ACTGACAATG AATCATGTAT ACAGTTTCTC CGTGTTTGAA 
CATACTATCT TCCCTTCTGA GTACCTCAGC AGCCACCTGG AGGCCAGACT CAACAGAGCC 
ATTGTGTGGC TGGCTGGCTA CAATGCCACG ACCCAGGAGA TCGTACGGCC TTCTGAGCTG 
TTGGCAGGAG TCAAAGCATA CATTGGTTTC ATACAGTCAC TGGCCCAGTT TTTGGGTGCA 
GATGCTTCCA GAGTCATCCG CAACGCCCTC CTGCAGCAGA CACAACCACT GGATTCCTGT 
GGGGAACAGA CAATCACCAC ACTCTACACA AACTGGTACC TGGAAAGTCT GCTTAGACAG 
GCAAGCAGTG GGACCATCAT CCTCTCCCCA GCCATGCAGG CCTTCGTCAG CCTGCCCAGA 
GAAGGGGAGC AGAACTTCAG TGCAGAGGAG TTCTCTGACA TCTCTGAGAT GCGGGCCTTG 
GCAGAACTCC TGGGCCCCTA TGGCATGAAG TTCCTGAGTG AAAACCTGAT GTGGCATGTG 
ACCTCTCAGA TTGTGGAGCT GAAGAAGCTG GTGGTGGAAA ACATGGACAT ACTTGTTCAG 
ATCAGATCCA ACTTTAGCAA GCCGGACTTG ATGGCTTCCC TGCTGCCCCA GCTGACAGGG 
GCTGAAAATG TGCTAAAGCG CATGACCATC ATTGGGGTTA TCCTCAGTTT CAGGGCCATG 
GCCCAAGAGG GACTTCGGGA GGTTTTCTCC TCCCACTGCC CATTTCTTAT GGGTCCCATT 
GAGTGCTTGA AGGAGTTTGT CACTCCAGAC ACAGACATCA AGGTGACCTT GAGTATCTTT 
GAGCTGGCAT CTGCTGCAGG TGTGGGCTGT GACATTGACC CAGCCTTGGT GGCTGCCATT 
GCTAATCTGA AAGCTGATAC TTCATCTCCT GAGGAGGAAT ATAAGGTGGC CTGCCTGCTC 
TTGATCTTTC TGGCAGTTTC CCTCCCACTC CTTGCCACTG ACCCTTCTTC CTTTTATAGC 
ATTGAGAAGG ATGGTTACAA CAACAATATT CATTGCTTGA CCAAAGCCAT CATCCAGGTG 
TCTGCTGCCC TCTTCACGCT CTACAACAAG AACATTGAAA CTCACCTCAA GGAATTTCTG 
GTGGTGGCCT CTGTCAGCCT CTTGCAGCTG GGCCAGGAGA CTGACAAGCT TAAAACCAGA 
AATCGAGAAT CCATTTCTCT GCTCATGCGC TTGGTGGTGG AGGAGTCATC CTTCCTGACC 
CTGGACATGC TGGAGTCCTG TTTCCCTTAT GTCCTGCTTC GAAATGCCTA TCGGGAGGTG 
TCTCGGGCCT TCCACCTAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005328.2
CDS	39..3422
Translation	MSLTSAYQHKLAEKLTILNDRGQGVLIRMYNIKKTCSDPKSKPPFLLEKSMEPSLKYINKKFPNIDVRNSTQHLGPVHREKAEIIRFLTNYYQSFVDVMEFRDHVYELLNTIDACQCHFDINLNFDFTRSYLDLIVTYTSVILLLSRIEDRRILIGMYNCAHEMLHGHGDPSFARLGQMVLEYDHPLKKLTEEFGPHTKAVSGALLSLHFLFVRRNQGAEQWRSAQLLSLISNPPAMINPANSDTMACEYLSVEVMERWIIIGFLLCHGCLNSNSQCQKLWKLCLQGSLYITLIREDVLQVHKVTEDLFSSLKGYGKRVADIKESKEHVIANSGQFHCQRRQFLRMAVKELETVLADEPGLLGPKALFAFMALSFIRDEVTWLVRHTENVTKTKTPEDYADSSIAELLFLLEGIRSLVRRHIKVIQQYHLQYLARFDALVLSDIIQNLSVCPEEESIIMSSFVSILSSLNLKQVDNGEKFEFSGLRLDWFRLQAYTSVAKAPLHLHENPDLAKVMNLIVFHSRMLDSVEKLLVETSDLSTFCFHLRIFEKMFAMTLEESAMLRYAIAFPLICAHFVHCTHEMCPEEYPHLKNHGLHHCNSFLEELAKQTSNCVLEICAEQRNLSEQLLPKHCATTISKAKNKKTRKQRQTPRKGEPERDKPGAESHRKNRSIVTNMDKLHLNLTELALTMNHVYSFSVFEHTIFPSEYLSSHLEARLNRAIVWLAGYNATTQEIVRPSELLAGVKAYIGFIQSLAQFLGADASRVIRNALLQQTQPLDSCGEQTITTLYTNWYLESLLRQASSGTIILSPAMQAFVSLPREGEQNFSAEEFSDISEMRALAELLGPYGMKFLSENLMWHVTSQIVELKKLVVENMDILVQIRSNFSKPDLMASLLPQLTGAENVLKRMTIIGVILSFRAMAQEGLREVFSSHCPFLMGPIECLKEFVTPDTDIKVTLSIFELASAAGVGCDIDPALVAAIANLKADTSSPEEEYKVACLLLIFLAVSLPLLATDPSSFYSIEKDGYNNNIHCLTKAIIQVSAALFTLYNKNIETHLKEFLVVASVSLLQLGQETDKLKTRNRESISLLMRLVVEESSFLTLDMLESCFPYVLLRNAYREVSRAFHLN

Target ORF information:

RefSeq Version	NM_005337.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 1 like (NCKAP1L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005337.5

ORF Insert Sequence:

ATGTCTTTGA CATCTGCTTA CCAGCATAAA TTAGCAGAGA AGCTCACTAT CCTGAATGAT 
CGCGGTCAGG GGGTTCTCAT CCGTATGTAT AACATCAAGA AGACTTGTTC AGACCCCAAA 
TCTAAGCCAC CTTTCTTACT GGAAAAGTCC ATGGAACCAT CTCTCAAGTA TATCAACAAG 
AAATTTCCCA ACATAGATGT CCGAAACAGC ACGCAACATT TAGGACCAGT ACATCGTGAA 
AAAGCCGAGA TAATTAGATT CCTCACCAAC TACTACCAGT CATTTGTGGA TGTCATGGAA 
TTTCGGGATC ATGTATATGA ACTTCTCAAC ACCATTGATG CCTGCCAGTG CCATTTTGAT 
ATCAATCTCA ACTTTGATTT CACTCGGAGT TACCTGGACT TGATTGTAAC TTACACCTCA 
GTCATTTTAC TTCTGTCACG GATTGAAGAT CGGCGGATAC TCATTGGCAT GTACAATTGT 
GCCCATGAGA TGCTGCATGG GCATGGTGAC CCCAGTTTTG CCCGTCTGGG TCAGATGGTC 
TTGGAGTATG ACCACCCTCT GAAGAAGCTG ACAGAAGAGT TTGGGCCTCA CACAAAGGCT 
GTGAGTGGAG CCCTCCTCTC TTTGCATTTC CTCTTTGTCC GAAGAAACCA GGGGGCTGAG 
CAGTGGCGCA GTGCCCAACT TCTAAGCCTC ATCAGCAACC CCCCAGCCAT GATTAACCCT 
GCTAATTCAG ATACAATGGC CTGTGAGTAT CTGTCTGTGG AAGTAATGGA GCGCTGGATT 
ATCATTGGGT TTCTTCTTTG TCATGGGTGC CTCAACTCCA ATAGCCAGTG CCAGAAGCTG 
TGGAAGCTGT GTCTGCAGGG CTCCCTCTAC ATCACCCTTA TCCGTGAGGA TGTGCTGCAG 
GTGCACAAAG TCACCGAGGA CCTGTTTAGC AGTTTGAAAG GGTATGGCAA GAGAGTGGCA 
GACATAAAGG AGAGCAAGGA ACATGTAATT GCAAACAGTG GCCAGTTTCA TTGTCAACGG 
CGGCAATTTC TGCGGATGGC AGTGAAGGAG CTGGAGACTG TGTTGGCTGA TGAACCGGGA 
CTACTGGGTC CTAAGGCTCT TTTTGCTTTC ATGGCCCTGT CCTTCATTCG TGATGAGGTC 
ACCTGGCTGG TTCGCCACAC AGAGAATGTC ACCAAGACAA AGACACCTGA GGACTATGCT 
GACTCGAGCA TTGCAGAGCT ACTTTTCTTG TTGGAGGGGA TTAGGTCTCT GGTCCGAAGA 
CACATCAAAG TGATACAGCA ATACCACCTT CAGTACTTGG CAAGATTTGA TGCTCTTGTG 
CTCAGTGACA TCATTCAGAA CTTGTCTGTG TGTCCAGAGG AGGAGTCCAT CATCATGTCC 
TCATTCGTCA GTATCCTCTC CTCTCTGAAT CTCAAACAAG TTGATAATGG AGAAAAATTT 
GAATTCTCAG GATTGAGGCT GGACTGGTTC CGCCTACAGG CATACACTAG CGTGGCTAAG 
GCCCCTCTGC ACCTGCATGA GAACCCTGAC TTAGCCAAGG TGATGAACCT CATTGTCTTC 
CACTCCCGAA TGCTGGACTC CGTAGAAAAA TTGCTGGTGG AAACTTCTGA TCTGTCTACT 
TTCTGCTTTC ATCTTCGTAT CTTTGAGAAG ATGTTTGCCA TGACCTTGGA GGAATCTGCC 
ATGTTGCGTT ATGCCATTGC TTTCCCCCTG ATTTGTGCTC ACTTTGTCCA CTGCACTCAT 
GAGATGTGCC CAGAGGAGTA CCCCCACCTC AAGAACCATG GTCTTCACCA CTGCAACTCC 
TTCCTGGAAG AGTTGGCCAA GCAGACCAGC AATTGCGTCC TGGAGATCTG TGCTGAGCAG 
CGAAACCTGA GCGAGCAGCT TCTACCTAAG CACTGTGCCA CTACAATCAG CAAAGCCAAG 
AACAAGAAAA CCAGGAAGCA GAGGCAGACT CCCAGAAAAG GAGAGCCCGA GAGGGACAAG 
CCAGGAGCTG AGAGTCACCG GAAGAACCGC AGCATTGTCA CCAACATGGA CAAGCTACAC 
CTAAACTTGA CAGAACTGGC ACTGACAATG AATCATGTAT ACAGTTTCTC CGTGTTTGAA 
CATACTATCT TCCCTTCTGA GTACCTCAGC AGCCACCTGG AGGCCAGACT CAACAGAGCC 
ATTGTGTGGC TGGCTGGCTA CAATGCCACG ACCCAGGAGA TCGTACGGCC TTCTGAGCTG 
TTGGCAGGAG TCAAAGCATA CATTGGTTTC ATACAGTCAC TGGCCCAGTT TTTGGGTGCA 
GATGCTTCCA GAGTCATCCG CAACGCCCTC CTGCAGCAGA CACAACCACT GGATTCCTGT 
GGGGAACAGA CAATCACCAC ACTCTACACA AACTGGTACC TGGAAAGTCT GCTTAGACAG 
GCAAGCAGTG GGACCATCAT CCTCTCCCCA GCCATGCAGG CCTTCGTCAG CCTGCCCAGA 
GAAGGGGAGC AGAACTTCAG TGCAGAGGAG TTCTCTGACA TCTCTGAGAT GCGGGCCTTG 
GCAGAACTCC TGGGCCCCTA TGGCATGAAG TTCCTGAGTG AAAACCTGAT GTGGCATGTG 
ACCTCTCAGA TTGTGGAGCT GAAGAAGCTG GTGGTGGAAA ACATGGACAT ACTTGTTCAG 
ATCAGATCCA ACTTTAGCAA GCCGGACTTG ATGGCTTCCC TGCTGCCCCA GCTGACAGGG 
GCTGAAAATG TGCTAAAGCG CATGACCATC ATTGGGGTTA TCCTCAGTTT CAGGGCCATG 
GCCCAAGAGG GACTTCGGGA GGTTTTCTCC TCCCACTGCC CATTTCTTAT GGGTCCCATT 
GAGTGCTTGA AGGAGTTTGT CACTCCAGAC ACAGACATCA AGGTGACCTT GAGTATCTTT 
GAGCTGGCAT CTGCTGCAGG TGTGGGCTGT GACATTGACC CAGCCTTGGT GGCTGCCATT 
GCTAATCTGA AAGCTGATAC TTCATCTCCT GAGGAGGAAT ATAAGGTGGC CTGCCTGCTC 
TTGATCTTTC TGGCAGTTTC CCTCCCACTC CTTGCCACTG ACCCTTCTTC CTTTTATAGC 
ATTGAGAAGG ATGGTTACAA CAACAATATT CATTGCTTGA CCAAAGCCAT CATCCAGGTG 
TCTGCTGCCC TCTTCACGCT CTACAACAAG AACATTGAAA CTCACCTCAA GGAATTTCTG 
GTGGTGGCCT CTGTCAGCCT CTTGCAGCTG GGCCAGGAGA CTGACAAGCT TAAAACCAGA 
AATCGAGAAT CCATTTCTCT GCTCATGCGC TTGGTGGTGG AGGAGTCATC CTTCCTGACC 
CTGGACATGC TGGAGTCCTG TTTCCCTTAT GTCCTGCTTC GAAATGCCTA TCGGGAGGTG 
TCTCGGGCCT TCCACCTAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1L ( NM_005337.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005328.2 Homo sapiens NCK associated protein 1 like (NCKAP1L), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31086
Clone ID Related Accession (Same CDS sequence)	NM_005337.5 , NM_005337.4
Accession Version	NM_005337.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	nck-associated protein 1-like isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA820115.1, BC093769.2, BC093771.1, M58285.1 and AW575485.1. On Jul 25, 2007 this sequence version replaced NM_005337.3. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803613.269017.1, M58285.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTTGA CATCTGCTTA CCAGCATAAA TTAGCAGAGA AGCTCACTAT CCTGAATGAT 
CGCGGTCAGG GGGTTCTCAT CCGTATGTAT AACATCAAGA AGACTTGTTC AGACCCCAAA 
TCTAAGCCAC CTTTCTTACT GGAAAAGTCC ATGGAACCAT CTCTCAAGTA TATCAACAAG 
AAATTTCCCA ACATAGATGT CCGAAACAGC ACGCAACATT TAGGACCAGT ACATCGTGAA 
AAAGCCGAGA TAATTAGATT CCTCACCAAC TACTACCAGT CATTTGTGGA TGTCATGGAA 
TTTCGGGATC ATGTATATGA ACTTCTCAAC ACCATTGATG CCTGCCAGTG CCATTTTGAT 
ATCAATCTCA ACTTTGATTT CACTCGGAGT TACCTGGACT TGATTGTAAC TTACACCTCA 
GTCATTTTAC TTCTGTCACG GATTGAAGAT CGGCGGATAC TCATTGGCAT GTACAATTGT 
GCCCATGAGA TGCTGCATGG GCATGGTGAC CCCAGTTTTG CCCGTCTGGG TCAGATGGTC 
TTGGAGTATG ACCACCCTCT GAAGAAGCTG ACAGAAGAGT TTGGGCCTCA CACAAAGGCT 
GTGAGTGGAG CCCTCCTCTC TTTGCATTTC CTCTTTGTCC GAAGAAACCA GGGGGCTGAG 
CAGTGGCGCA GTGCCCAACT TCTAAGCCTC ATCAGCAACC CCCCAGCCAT GATTAACCCT 
GCTAATTCAG ATACAATGGC CTGTGAGTAT CTGTCTGTGG AAGTAATGGA GCGCTGGATT 
ATCATTGGGT TTCTTCTTTG TCATGGGTGC CTCAACTCCA ATAGCCAGTG CCAGAAGCTG 
TGGAAGCTGT GTCTGCAGGG CTCCCTCTAC ATCACCCTTA TCCGTGAGGA TGTGCTGCAG 
GTGCACAAAG TCACCGAGGA CCTGTTTAGC AGTTTGAAAG GGTATGGCAA GAGAGTGGCA 
GACATAAAGG AGAGCAAGGA ACATGTAATT GCAAACAGTG GCCAGTTTCA TTGTCAACGG 
CGGCAATTTC TGCGGATGGC AGTGAAGGAG CTGGAGACTG TGTTGGCTGA TGAACCGGGA 
CTACTGGGTC CTAAGGCTCT TTTTGCTTTC ATGGCCCTGT CCTTCATTCG TGATGAGGTC 
ACCTGGCTGG TTCGCCACAC AGAGAATGTC ACCAAGACAA AGACACCTGA GGACTATGCT 
GACTCGAGCA TTGCAGAGCT ACTTTTCTTG TTGGAGGGGA TTAGGTCTCT GGTCCGAAGA 
CACATCAAAG TGATACAGCA ATACCACCTT CAGTACTTGG CAAGATTTGA TGCTCTTGTG 
CTCAGTGACA TCATTCAGAA CTTGTCTGTG TGTCCAGAGG AGGAGTCCAT CATCATGTCC 
TCATTCGTCA GTATCCTCTC CTCTCTGAAT CTCAAACAAG TTGATAATGG AGAAAAATTT 
GAATTCTCAG GATTGAGGCT GGACTGGTTC CGCCTACAGG CATACACTAG CGTGGCTAAG 
GCCCCTCTGC ACCTGCATGA GAACCCTGAC TTAGCCAAGG TGATGAACCT CATTGTCTTC 
CACTCCCGAA TGCTGGACTC CGTAGAAAAA TTGCTGGTGG AAACTTCTGA TCTGTCTACT 
TTCTGCTTTC ATCTTCGTAT CTTTGAGAAG ATGTTTGCCA TGACCTTGGA GGAATCTGCC 
ATGTTGCGTT ATGCCATTGC TTTCCCCCTG ATTTGTGCTC ACTTTGTCCA CTGCACTCAT 
GAGATGTGCC CAGAGGAGTA CCCCCACCTC AAGAACCATG GTCTTCACCA CTGCAACTCC 
TTCCTGGAAG AGTTGGCCAA GCAGACCAGC AATTGCGTCC TGGAGATCTG TGCTGAGCAG 
CGAAACCTGA GCGAGCAGCT TCTACCTAAG CACTGTGCCA CTACAATCAG CAAAGCCAAG 
AACAAGAAAA CCAGGAAGCA GAGGCAGACT CCCAGAAAAG GAGAGCCCGA GAGGGACAAG 
CCAGGAGCTG AGAGTCACCG GAAGAACCGC AGCATTGTCA CCAACATGGA CAAGCTACAC 
CTAAACTTGA CAGAACTGGC ACTGACAATG AATCATGTAT ACAGTTTCTC CGTGTTTGAA 
CATACTATCT TCCCTTCTGA GTACCTCAGC AGCCACCTGG AGGCCAGACT CAACAGAGCC 
ATTGTGTGGC TGGCTGGCTA CAATGCCACG ACCCAGGAGA TCGTACGGCC TTCTGAGCTG 
TTGGCAGGAG TCAAAGCATA CATTGGTTTC ATACAGTCAC TGGCCCAGTT TTTGGGTGCA 
GATGCTTCCA GAGTCATCCG CAACGCCCTC CTGCAGCAGA CACAACCACT GGATTCCTGT 
GGGGAACAGA CAATCACCAC ACTCTACACA AACTGGTACC TGGAAAGTCT GCTTAGACAG 
GCAAGCAGTG GGACCATCAT CCTCTCCCCA GCCATGCAGG CCTTCGTCAG CCTGCCCAGA 
GAAGGGGAGC AGAACTTCAG TGCAGAGGAG TTCTCTGACA TCTCTGAGAT GCGGGCCTTG 
GCAGAACTCC TGGGCCCCTA TGGCATGAAG TTCCTGAGTG AAAACCTGAT GTGGCATGTG 
ACCTCTCAGA TTGTGGAGCT GAAGAAGCTG GTGGTGGAAA ACATGGACAT ACTTGTTCAG 
ATCAGATCCA ACTTTAGCAA GCCGGACTTG ATGGCTTCCC TGCTGCCCCA GCTGACAGGG 
GCTGAAAATG TGCTAAAGCG CATGACCATC ATTGGGGTTA TCCTCAGTTT CAGGGCCATG 
GCCCAAGAGG GACTTCGGGA GGTTTTCTCC TCCCACTGCC CATTTCTTAT GGGTCCCATT 
GAGTGCTTGA AGGAGTTTGT CACTCCAGAC ACAGACATCA AGGTGACCTT GAGTATCTTT 
GAGCTGGCAT CTGCTGCAGG TGTGGGCTGT GACATTGACC CAGCCTTGGT GGCTGCCATT 
GCTAATCTGA AAGCTGATAC TTCATCTCCT GAGGAGGAAT ATAAGGTGGC CTGCCTGCTC 
TTGATCTTTC TGGCAGTTTC CCTCCCACTC CTTGCCACTG ACCCTTCTTC CTTTTATAGC 
ATTGAGAAGG ATGGTTACAA CAACAATATT CATTGCTTGA CCAAAGCCAT CATCCAGGTG 
TCTGCTGCCC TCTTCACGCT CTACAACAAG AACATTGAAA CTCACCTCAA GGAATTTCTG 
GTGGTGGCCT CTGTCAGCCT CTTGCAGCTG GGCCAGGAGA CTGACAAGCT TAAAACCAGA 
AATCGAGAAT CCATTTCTCT GCTCATGCGC TTGGTGGTGG AGGAGTCATC CTTCCTGACC 
CTGGACATGC TGGAGTCCTG TTTCCCTTAT GTCCTGCTTC GAAATGCCTA TCGGGAGGTG 
TCTCGGGCCT TCCACCTAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005328.2
CDS	80..3463
Translation	MSLTSAYQHKLAEKLTILNDRGQGVLIRMYNIKKTCSDPKSKPPFLLEKSMEPSLKYINKKFPNIDVRNSTQHLGPVHREKAEIIRFLTNYYQSFVDVMEFRDHVYELLNTIDACQCHFDINLNFDFTRSYLDLIVTYTSVILLLSRIEDRRILIGMYNCAHEMLHGHGDPSFARLGQMVLEYDHPLKKLTEEFGPHTKAVSGALLSLHFLFVRRNQGAEQWRSAQLLSLISNPPAMINPANSDTMACEYLSVEVMERWIIIGFLLCHGCLNSNSQCQKLWKLCLQGSLYITLIREDVLQVHKVTEDLFSSLKGYGKRVADIKESKEHVIANSGQFHCQRRQFLRMAVKELETVLADEPGLLGPKALFAFMALSFIRDEVTWLVRHTENVTKTKTPEDYADSSIAELLFLLEGIRSLVRRHIKVIQQYHLQYLARFDALVLSDIIQNLSVCPEEESIIMSSFVSILSSLNLKQVDNGEKFEFSGLRLDWFRLQAYTSVAKAPLHLHENPDLAKVMNLIVFHSRMLDSVEKLLVETSDLSTFCFHLRIFEKMFAMTLEESAMLRYAIAFPLICAHFVHCTHEMCPEEYPHLKNHGLHHCNSFLEELAKQTSNCVLEICAEQRNLSEQLLPKHCATTISKAKNKKTRKQRQTPRKGEPERDKPGAESHRKNRSIVTNMDKLHLNLTELALTMNHVYSFSVFEHTIFPSEYLSSHLEARLNRAIVWLAGYNATTQEIVRPSELLAGVKAYIGFIQSLAQFLGADASRVIRNALLQQTQPLDSCGEQTITTLYTNWYLESLLRQASSGTIILSPAMQAFVSLPREGEQNFSAEEFSDISEMRALAELLGPYGMKFLSENLMWHVTSQIVELKKLVVENMDILVQIRSNFSKPDLMASLLPQLTGAENVLKRMTIIGVILSFRAMAQEGLREVFSSHCPFLMGPIECLKEFVTPDTDIKVTLSIFELASAAGVGCDIDPALVAAIANLKADTSSPEEEYKVACLLLIFLAVSLPLLATDPSSFYSIEKDGYNNNIHCLTKAIIQVSAALFTLYNKNIETHLKEFLVVASVSLLQLGQETDKLKTRNRESISLLMRLVVEESSFLTLDMLESCFPYVLLRNAYREVSRAFHLN

Target ORF information:

RefSeq Version	NM_005337.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 1 like (NCKAP1L), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005337.4

ORF Insert Sequence:

ATGTCTTTGA CATCTGCTTA CCAGCATAAA TTAGCAGAGA AGCTCACTAT CCTGAATGAT 
CGCGGTCAGG GGGTTCTCAT CCGTATGTAT AACATCAAGA AGACTTGTTC AGACCCCAAA 
TCTAAGCCAC CTTTCTTACT GGAAAAGTCC ATGGAACCAT CTCTCAAGTA TATCAACAAG 
AAATTTCCCA ACATAGATGT CCGAAACAGC ACGCAACATT TAGGACCAGT ACATCGTGAA 
AAAGCCGAGA TAATTAGATT CCTCACCAAC TACTACCAGT CATTTGTGGA TGTCATGGAA 
TTTCGGGATC ATGTATATGA ACTTCTCAAC ACCATTGATG CCTGCCAGTG CCATTTTGAT 
ATCAATCTCA ACTTTGATTT CACTCGGAGT TACCTGGACT TGATTGTAAC TTACACCTCA 
GTCATTTTAC TTCTGTCACG GATTGAAGAT CGGCGGATAC TCATTGGCAT GTACAATTGT 
GCCCATGAGA TGCTGCATGG GCATGGTGAC CCCAGTTTTG CCCGTCTGGG TCAGATGGTC 
TTGGAGTATG ACCACCCTCT GAAGAAGCTG ACAGAAGAGT TTGGGCCTCA CACAAAGGCT 
GTGAGTGGAG CCCTCCTCTC TTTGCATTTC CTCTTTGTCC GAAGAAACCA GGGGGCTGAG 
CAGTGGCGCA GTGCCCAACT TCTAAGCCTC ATCAGCAACC CCCCAGCCAT GATTAACCCT 
GCTAATTCAG ATACAATGGC CTGTGAGTAT CTGTCTGTGG AAGTAATGGA GCGCTGGATT 
ATCATTGGGT TTCTTCTTTG TCATGGGTGC CTCAACTCCA ATAGCCAGTG CCAGAAGCTG 
TGGAAGCTGT GTCTGCAGGG CTCCCTCTAC ATCACCCTTA TCCGTGAGGA TGTGCTGCAG 
GTGCACAAAG TCACCGAGGA CCTGTTTAGC AGTTTGAAAG GGTATGGCAA GAGAGTGGCA 
GACATAAAGG AGAGCAAGGA ACATGTAATT GCAAACAGTG GCCAGTTTCA TTGTCAACGG 
CGGCAATTTC TGCGGATGGC AGTGAAGGAG CTGGAGACTG TGTTGGCTGA TGAACCGGGA 
CTACTGGGTC CTAAGGCTCT TTTTGCTTTC ATGGCCCTGT CCTTCATTCG TGATGAGGTC 
ACCTGGCTGG TTCGCCACAC AGAGAATGTC ACCAAGACAA AGACACCTGA GGACTATGCT 
GACTCGAGCA TTGCAGAGCT ACTTTTCTTG TTGGAGGGGA TTAGGTCTCT GGTCCGAAGA 
CACATCAAAG TGATACAGCA ATACCACCTT CAGTACTTGG CAAGATTTGA TGCTCTTGTG 
CTCAGTGACA TCATTCAGAA CTTGTCTGTG TGTCCAGAGG AGGAGTCCAT CATCATGTCC 
TCATTCGTCA GTATCCTCTC CTCTCTGAAT CTCAAACAAG TTGATAATGG AGAAAAATTT 
GAATTCTCAG GATTGAGGCT GGACTGGTTC CGCCTACAGG CATACACTAG CGTGGCTAAG 
GCCCCTCTGC ACCTGCATGA GAACCCTGAC TTAGCCAAGG TGATGAACCT CATTGTCTTC 
CACTCCCGAA TGCTGGACTC CGTAGAAAAA TTGCTGGTGG AAACTTCTGA TCTGTCTACT 
TTCTGCTTTC ATCTTCGTAT CTTTGAGAAG ATGTTTGCCA TGACCTTGGA GGAATCTGCC 
ATGTTGCGTT ATGCCATTGC TTTCCCCCTG ATTTGTGCTC ACTTTGTCCA CTGCACTCAT 
GAGATGTGCC CAGAGGAGTA CCCCCACCTC AAGAACCATG GTCTTCACCA CTGCAACTCC 
TTCCTGGAAG AGTTGGCCAA GCAGACCAGC AATTGCGTCC TGGAGATCTG TGCTGAGCAG 
CGAAACCTGA GCGAGCAGCT TCTACCTAAG CACTGTGCCA CTACAATCAG CAAAGCCAAG 
AACAAGAAAA CCAGGAAGCA GAGGCAGACT CCCAGAAAAG GAGAGCCCGA GAGGGACAAG 
CCAGGAGCTG AGAGTCACCG GAAGAACCGC AGCATTGTCA CCAACATGGA CAAGCTACAC 
CTAAACTTGA CAGAACTGGC ACTGACAATG AATCATGTAT ACAGTTTCTC CGTGTTTGAA 
CATACTATCT TCCCTTCTGA GTACCTCAGC AGCCACCTGG AGGCCAGACT CAACAGAGCC 
ATTGTGTGGC TGGCTGGCTA CAATGCCACG ACCCAGGAGA TCGTACGGCC TTCTGAGCTG 
TTGGCAGGAG TCAAAGCATA CATTGGTTTC ATACAGTCAC TGGCCCAGTT TTTGGGTGCA 
GATGCTTCCA GAGTCATCCG CAACGCCCTC CTGCAGCAGA CACAACCACT GGATTCCTGT 
GGGGAACAGA CAATCACCAC ACTCTACACA AACTGGTACC TGGAAAGTCT GCTTAGACAG 
GCAAGCAGTG GGACCATCAT CCTCTCCCCA GCCATGCAGG CCTTCGTCAG CCTGCCCAGA 
GAAGGGGAGC AGAACTTCAG TGCAGAGGAG TTCTCTGACA TCTCTGAGAT GCGGGCCTTG 
GCAGAACTCC TGGGCCCCTA TGGCATGAAG TTCCTGAGTG AAAACCTGAT GTGGCATGTG 
ACCTCTCAGA TTGTGGAGCT GAAGAAGCTG GTGGTGGAAA ACATGGACAT ACTTGTTCAG 
ATCAGATCCA ACTTTAGCAA GCCGGACTTG ATGGCTTCCC TGCTGCCCCA GCTGACAGGG 
GCTGAAAATG TGCTAAAGCG CATGACCATC ATTGGGGTTA TCCTCAGTTT CAGGGCCATG 
GCCCAAGAGG GACTTCGGGA GGTTTTCTCC TCCCACTGCC CATTTCTTAT GGGTCCCATT 
GAGTGCTTGA AGGAGTTTGT CACTCCAGAC ACAGACATCA AGGTGACCTT GAGTATCTTT 
GAGCTGGCAT CTGCTGCAGG TGTGGGCTGT GACATTGACC CAGCCTTGGT GGCTGCCATT 
GCTAATCTGA AAGCTGATAC TTCATCTCCT GAGGAGGAAT ATAAGGTGGC CTGCCTGCTC 
TTGATCTTTC TGGCAGTTTC CCTCCCACTC CTTGCCACTG ACCCTTCTTC CTTTTATAGC 
ATTGAGAAGG ATGGTTACAA CAACAATATT CATTGCTTGA CCAAAGCCAT CATCCAGGTG 
TCTGCTGCCC TCTTCACGCT CTACAACAAG AACATTGAAA CTCACCTCAA GGAATTTCTG 
GTGGTGGCCT CTGTCAGCCT CTTGCAGCTG GGCCAGGAGA CTGACAAGCT TAAAACCAGA 
AATCGAGAAT CCATTTCTCT GCTCATGCGC TTGGTGGTGG AGGAGTCATC CTTCCTGACC 
CTGGACATGC TGGAGTCCTG TTTCCCTTAT GTCCTGCTTC GAAATGCCTA TCGGGAGGTG 
TCTCGGGCCT TCCACCTAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1L ( NM_001184976.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171905.1 Homo sapiens NCK associated protein 1 like (NCKAP1L), transcript variant 2, mRNA.

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CloneID	OHu55576
Clone ID Related Accession (Same CDS sequence)	NM_001184976.2 , NM_001184976.1
Accession Version	NM_001184976.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	nck-associated protein 1-like isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC079313.35, AK300783.1, AW575485.1 and AC068789.16. On May 31, 2019 this sequence version replaced NM_001184976.1. Transcript Variant: This variant (2) has an alternate 5' exon, resulting in a downstream in-frame AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter N-terminus, as compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK300783.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END##

ATGGAACCAT CTCTCAAGTA TATCAACAAG AAATTTCCCA ACATAGATGT CCGAAACAGC 
ACGCAACATT TAGGACCAGT ACATCGTGAA AAAGCCGAGA TAATTAGATT CCTCACCAAC 
TACTACCAGT CATTTGTGGA TGTCATGGAA TTTCGGGATC ATGTATATGA ACTTCTCAAC 
ACCATTGATG CCTGCCAGTG CCATTTTGAT ATCAATCTCA ACTTTGATTT CACTCGGAGT 
TACCTGGACT TGATTGTAAC TTACACCTCA GTCATTTTAC TTCTGTCACG GATTGAAGAT 
CGGCGGATAC TCATTGGCAT GTACAATTGT GCCCATGAGA TGCTGCATGG GCATGGTGAC 
CCCAGTTTTG CCCGTCTGGG TCAGATGGTC TTGGAGTATG ACCACCCTCT GAAGAAGCTG 
ACAGAAGAGT TTGGGCCTCA CACAAAGGCT GTGAGTGGAG CCCTCCTCTC TTTGCATTTC 
CTCTTTGTCC GAAGAAACCA GGGGGCTGAG CAGTGGCGCA GTGCCCAACT TCTAAGCCTC 
ATCAGCAACC CCCCAGCCAT GATTAACCCT GCTAATTCAG ATACAATGGC CTGTGAGTAT 
CTGTCTGTGG AAGTAATGGA GCGCTGGATT ATCATTGGGT TTCTTCTTTG TCATGGGTGC 
CTCAACTCCA ATAGCCAGTG CCAGAAGCTG TGGAAGCTGT GTCTGCAGGG CTCCCTCTAC 
ATCACCCTTA TCCGTGAGGA TGTGCTGCAG GTGCACAAAG TCACCGAGGA CCTGTTTAGC 
AGTTTGAAAG GGTATGGCAA GAGAGTGGCA GACATAAAGG AGAGCAAGGA ACATGTAATT 
GCAAACAGTG GCCAGTTTCA TTGTCAACGG CGGCAATTTC TGCGGATGGC AGTGAAGGAG 
CTGGAGACTG TGTTGGCTGA TGAACCGGGA CTACTGGGTC CTAAGGCTCT TTTTGCTTTC 
ATGGCCCTGT CCTTCATTCG TGATGAGGTC ACCTGGCTGG TTCGCCACAC AGAGAATGTC 
ACCAAGACAA AGACACCTGA GGACTATGCT GACTCGAGCA TTGCAGAGCT ACTTTTCTTG 
TTGGAGGGGA TTAGGTCTCT GGTCCGAAGA CACATCAAAG TGATACAGCA ATACCACCTT 
CAGTACTTGG CAAGATTTGA TGCTCTTGTG CTCAGTGACA TCATTCAGAA CTTGTCTGTG 
TGTCCAGAGG AGGAGTCCAT CATCATGTCC TCATTCGTCA GTATCCTCTC CTCTCTGAAT 
CTCAAACAAG TTGATAATGG AGAAAAATTT GAATTCTCAG GATTGAGGCT GGACTGGTTC 
CGCCTACAGG CATACACTAG CGTGGCTAAG GCCCCTCTGC ACCTGCATGA GAACCCTGAC 
TTAGCCAAGG TGATGAACCT CATTGTCTTC CACTCCCGAA TGCTGGACTC CGTAGAAAAA 
TTGCTGGTGG AAACTTCTGA TCTGTCTACT TTCTGCTTTC ATCTTCGTAT CTTTGAGAAG 
ATGTTTGCCA TGACCTTGGA GGAATCTGCC ATGTTGCGTT ATGCCATTGC TTTCCCCCTG 
ATTTGTGCTC ACTTTGTCCA CTGCACTCAT GAGATGTGCC CAGAGGAGTA CCCCCACCTC 
AAGAACCATG GTCTTCACCA CTGCAACTCC TTCCTGGAAG AGTTGGCCAA GCAGACCAGC 
AATTGCGTCC TGGAGATCTG TGCTGAGCAG CGAAACCTGA GCGAGCAGCT TCTACCTAAG 
CACTGTGCCA CTACAATCAG CAAAGCCAAG AACAAGAAAA CCAGGAAGCA GAGGCAGACT 
CCCAGAAAAG GAGAGCCCGA GAGGGACAAG CCAGGAGCTG AGAGTCACCG GAAGAACCGC 
AGCATTGTCA CCAACATGGA CAAGCTACAC CTAAACTTGA CAGAACTGGC ACTGACAATG 
AATCATGTAT ACAGTTTCTC CGTGTTTGAA CATACTATCT TCCCTTCTGA GTACCTCAGC 
AGCCACCTGG AGGCCAGACT CAACAGAGCC ATTGTGTGGC TGGCTGGCTA CAATGCCACG 
ACCCAGGAGA TCGTACGGCC TTCTGAGCTG TTGGCAGGAG TCAAAGCATA CATTGGTTTC 
ATACAGTCAC TGGCCCAGTT TTTGGGTGCA GATGCTTCCA GAGTCATCCG CAACGCCCTC 
CTGCAGCAGA CACAACCACT GGATTCCTGT GGGGAACAGA CAATCACCAC ACTCTACACA 
AACTGGTACC TGGAAAGTCT GCTTAGACAG GCAAGCAGTG GGACCATCAT CCTCTCCCCA 
GCCATGCAGG CCTTCGTCAG CCTGCCCAGA GAAGGGGAGC AGAACTTCAG TGCAGAGGAG 
TTCTCTGACA TCTCTGAGAT GCGGGCCTTG GCAGAACTCC TGGGCCCCTA TGGCATGAAG 
TTCCTGAGTG AAAACCTGAT GTGGCATGTG ACCTCTCAGA TTGTGGAGCT GAAGAAGCTG 
GTGGTGGAAA ACATGGACAT ACTTGTTCAG ATCAGATCCA ACTTTAGCAA GCCGGACTTG 
ATGGCTTCCC TGCTGCCCCA GCTGACAGGG GCTGAAAATG TGCTAAAGCG CATGACCATC 
ATTGGGGTTA TCCTCAGTTT CAGGGCCATG GCCCAAGAGG GACTTCGGGA GGTTTTCTCC 
TCCCACTGCC CATTTCTTAT GGGTCCCATT GAGTGCTTGA AGGAGTTTGT CACTCCAGAC 
ACAGACATCA AGGTGACCTT GAGTATCTTT GAGCTGGCAT CTGCTGCAGG TGTGGGCTGT 
GACATTGACC CAGCCTTGGT GGCTGCCATT GCTAATCTGA AAGCTGATAC TTCATCTCCT 
GAGGAGGAAT ATAAGGTGGC CTGCCTGCTC TTGATCTTTC TGGCAGTTTC CCTCCCACTC 
CTTGCCACTG ACCCTTCTTC CTTTTATAGC ATTGAGAAGG ATGGTTACAA CAACAATATT 
CATTGCTTGA CCAAAGCCAT CATCCAGGTG TCTGCTGCCC TCTTCACGCT CTACAACAAG 
AACATTGAAA CTCACCTCAA GGAATTTCTG GTGGTGGCCT CTGTCAGCCT CTTGCAGCTG 
GGCCAGGAGA CTGACAAGCT TAAAACCAGA AATCGAGAAT CCATTTCTCT GCTCATGCGC 
TTGGTGGTGG AGGAGTCATC CTTCCTGACC CTGGACATGC TGGAGTCCTG TTTCCCTTAT 
GTCCTGCTTC GAAATGCCTA TCGGGAGGTG TCTCGGGCCT TCCACCTAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171905.1
CDS	107..3340
Translation	MEPSLKYINKKFPNIDVRNSTQHLGPVHREKAEIIRFLTNYYQSFVDVMEFRDHVYELLNTIDACQCHFDINLNFDFTRSYLDLIVTYTSVILLLSRIEDRRILIGMYNCAHEMLHGHGDPSFARLGQMVLEYDHPLKKLTEEFGPHTKAVSGALLSLHFLFVRRNQGAEQWRSAQLLSLISNPPAMINPANSDTMACEYLSVEVMERWIIIGFLLCHGCLNSNSQCQKLWKLCLQGSLYITLIREDVLQVHKVTEDLFSSLKGYGKRVADIKESKEHVIANSGQFHCQRRQFLRMAVKELETVLADEPGLLGPKALFAFMALSFIRDEVTWLVRHTENVTKTKTPEDYADSSIAELLFLLEGIRSLVRRHIKVIQQYHLQYLARFDALVLSDIIQNLSVCPEEESIIMSSFVSILSSLNLKQVDNGEKFEFSGLRLDWFRLQAYTSVAKAPLHLHENPDLAKVMNLIVFHSRMLDSVEKLLVETSDLSTFCFHLRIFEKMFAMTLEESAMLRYAIAFPLICAHFVHCTHEMCPEEYPHLKNHGLHHCNSFLEELAKQTSNCVLEICAEQRNLSEQLLPKHCATTISKAKNKKTRKQRQTPRKGEPERDKPGAESHRKNRSIVTNMDKLHLNLTELALTMNHVYSFSVFEHTIFPSEYLSSHLEARLNRAIVWLAGYNATTQEIVRPSELLAGVKAYIGFIQSLAQFLGADASRVIRNALLQQTQPLDSCGEQTITTLYTNWYLESLLRQASSGTIILSPAMQAFVSLPREGEQNFSAEEFSDISEMRALAELLGPYGMKFLSENLMWHVTSQIVELKKLVVENMDILVQIRSNFSKPDLMASLLPQLTGAENVLKRMTIIGVILSFRAMAQEGLREVFSSHCPFLMGPIECLKEFVTPDTDIKVTLSIFELASAAGVGCDIDPALVAAIANLKADTSSPEEEYKVACLLLIFLAVSLPLLATDPSSFYSIEKDGYNNNIHCLTKAIIQVSAALFTLYNKNIETHLKEFLVVASVSLLQLGQETDKLKTRNRESISLLMRLVVEESSFLTLDMLESCFPYVLLRNAYREVSRAFHLN

Target ORF information:

RefSeq Version	NM_001184976.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 1 like (NCKAP1L), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184976.2

ORF Insert Sequence:

ATGGAACCAT CTCTCAAGTA TATCAACAAG AAATTTCCCA ACATAGATGT CCGAAACAGC 
ACGCAACATT TAGGACCAGT ACATCGTGAA AAAGCCGAGA TAATTAGATT CCTCACCAAC 
TACTACCAGT CATTTGTGGA TGTCATGGAA TTTCGGGATC ATGTATATGA ACTTCTCAAC 
ACCATTGATG CCTGCCAGTG CCATTTTGAT ATCAATCTCA ACTTTGATTT CACTCGGAGT 
TACCTGGACT TGATTGTAAC TTACACCTCA GTCATTTTAC TTCTGTCACG GATTGAAGAT 
CGGCGGATAC TCATTGGCAT GTACAATTGT GCCCATGAGA TGCTGCATGG GCATGGTGAC 
CCCAGTTTTG CCCGTCTGGG TCAGATGGTC TTGGAGTATG ACCACCCTCT GAAGAAGCTG 
ACAGAAGAGT TTGGGCCTCA CACAAAGGCT GTGAGTGGAG CCCTCCTCTC TTTGCATTTC 
CTCTTTGTCC GAAGAAACCA GGGGGCTGAG CAGTGGCGCA GTGCCCAACT TCTAAGCCTC 
ATCAGCAACC CCCCAGCCAT GATTAACCCT GCTAATTCAG ATACAATGGC CTGTGAGTAT 
CTGTCTGTGG AAGTAATGGA GCGCTGGATT ATCATTGGGT TTCTTCTTTG TCATGGGTGC 
CTCAACTCCA ATAGCCAGTG CCAGAAGCTG TGGAAGCTGT GTCTGCAGGG CTCCCTCTAC 
ATCACCCTTA TCCGTGAGGA TGTGCTGCAG GTGCACAAAG TCACCGAGGA CCTGTTTAGC 
AGTTTGAAAG GGTATGGCAA GAGAGTGGCA GACATAAAGG AGAGCAAGGA ACATGTAATT 
GCAAACAGTG GCCAGTTTCA TTGTCAACGG CGGCAATTTC TGCGGATGGC AGTGAAGGAG 
CTGGAGACTG TGTTGGCTGA TGAACCGGGA CTACTGGGTC CTAAGGCTCT TTTTGCTTTC 
ATGGCCCTGT CCTTCATTCG TGATGAGGTC ACCTGGCTGG TTCGCCACAC AGAGAATGTC 
ACCAAGACAA AGACACCTGA GGACTATGCT GACTCGAGCA TTGCAGAGCT ACTTTTCTTG 
TTGGAGGGGA TTAGGTCTCT GGTCCGAAGA CACATCAAAG TGATACAGCA ATACCACCTT 
CAGTACTTGG CAAGATTTGA TGCTCTTGTG CTCAGTGACA TCATTCAGAA CTTGTCTGTG 
TGTCCAGAGG AGGAGTCCAT CATCATGTCC TCATTCGTCA GTATCCTCTC CTCTCTGAAT 
CTCAAACAAG TTGATAATGG AGAAAAATTT GAATTCTCAG GATTGAGGCT GGACTGGTTC 
CGCCTACAGG CATACACTAG CGTGGCTAAG GCCCCTCTGC ACCTGCATGA GAACCCTGAC 
TTAGCCAAGG TGATGAACCT CATTGTCTTC CACTCCCGAA TGCTGGACTC CGTAGAAAAA 
TTGCTGGTGG AAACTTCTGA TCTGTCTACT TTCTGCTTTC ATCTTCGTAT CTTTGAGAAG 
ATGTTTGCCA TGACCTTGGA GGAATCTGCC ATGTTGCGTT ATGCCATTGC TTTCCCCCTG 
ATTTGTGCTC ACTTTGTCCA CTGCACTCAT GAGATGTGCC CAGAGGAGTA CCCCCACCTC 
AAGAACCATG GTCTTCACCA CTGCAACTCC TTCCTGGAAG AGTTGGCCAA GCAGACCAGC 
AATTGCGTCC TGGAGATCTG TGCTGAGCAG CGAAACCTGA GCGAGCAGCT TCTACCTAAG 
CACTGTGCCA CTACAATCAG CAAAGCCAAG AACAAGAAAA CCAGGAAGCA GAGGCAGACT 
CCCAGAAAAG GAGAGCCCGA GAGGGACAAG CCAGGAGCTG AGAGTCACCG GAAGAACCGC 
AGCATTGTCA CCAACATGGA CAAGCTACAC CTAAACTTGA CAGAACTGGC ACTGACAATG 
AATCATGTAT ACAGTTTCTC CGTGTTTGAA CATACTATCT TCCCTTCTGA GTACCTCAGC 
AGCCACCTGG AGGCCAGACT CAACAGAGCC ATTGTGTGGC TGGCTGGCTA CAATGCCACG 
ACCCAGGAGA TCGTACGGCC TTCTGAGCTG TTGGCAGGAG TCAAAGCATA CATTGGTTTC 
ATACAGTCAC TGGCCCAGTT TTTGGGTGCA GATGCTTCCA GAGTCATCCG CAACGCCCTC 
CTGCAGCAGA CACAACCACT GGATTCCTGT GGGGAACAGA CAATCACCAC ACTCTACACA 
AACTGGTACC TGGAAAGTCT GCTTAGACAG GCAAGCAGTG GGACCATCAT CCTCTCCCCA 
GCCATGCAGG CCTTCGTCAG CCTGCCCAGA GAAGGGGAGC AGAACTTCAG TGCAGAGGAG 
TTCTCTGACA TCTCTGAGAT GCGGGCCTTG GCAGAACTCC TGGGCCCCTA TGGCATGAAG 
TTCCTGAGTG AAAACCTGAT GTGGCATGTG ACCTCTCAGA TTGTGGAGCT GAAGAAGCTG 
GTGGTGGAAA ACATGGACAT ACTTGTTCAG ATCAGATCCA ACTTTAGCAA GCCGGACTTG 
ATGGCTTCCC TGCTGCCCCA GCTGACAGGG GCTGAAAATG TGCTAAAGCG CATGACCATC 
ATTGGGGTTA TCCTCAGTTT CAGGGCCATG GCCCAAGAGG GACTTCGGGA GGTTTTCTCC 
TCCCACTGCC CATTTCTTAT GGGTCCCATT GAGTGCTTGA AGGAGTTTGT CACTCCAGAC 
ACAGACATCA AGGTGACCTT GAGTATCTTT GAGCTGGCAT CTGCTGCAGG TGTGGGCTGT 
GACATTGACC CAGCCTTGGT GGCTGCCATT GCTAATCTGA AAGCTGATAC TTCATCTCCT 
GAGGAGGAAT ATAAGGTGGC CTGCCTGCTC TTGATCTTTC TGGCAGTTTC CCTCCCACTC 
CTTGCCACTG ACCCTTCTTC CTTTTATAGC ATTGAGAAGG ATGGTTACAA CAACAATATT 
CATTGCTTGA CCAAAGCCAT CATCCAGGTG TCTGCTGCCC TCTTCACGCT CTACAACAAG 
AACATTGAAA CTCACCTCAA GGAATTTCTG GTGGTGGCCT CTGTCAGCCT CTTGCAGCTG 
GGCCAGGAGA CTGACAAGCT TAAAACCAGA AATCGAGAAT CCATTTCTCT GCTCATGCGC 
TTGGTGGTGG AGGAGTCATC CTTCCTGACC CTGGACATGC TGGAGTCCTG TTTCCCTTAT 
GTCCTGCTTC GAAATGCCTA TCGGGAGGTG TCTCGGGCCT TCCACCTAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1L ( NM_001184976.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171905.1 Homo sapiens NCK associated protein 1 like (NCKAP1L), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55576
Clone ID Related Accession (Same CDS sequence)	NM_001184976.2 , NM_001184976.1
Accession Version	NM_001184976.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	nck-associated protein 1-like isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300783.1 and AW575485.1. Transcript Variant: This variant (2) has an alternate 5' exon, resulting in a downstream in-frame AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter N-terminus, as compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK300783.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END##

ATGGAACCAT CTCTCAAGTA TATCAACAAG AAATTTCCCA ACATAGATGT CCGAAACAGC 
ACGCAACATT TAGGACCAGT ACATCGTGAA AAAGCCGAGA TAATTAGATT CCTCACCAAC 
TACTACCAGT CATTTGTGGA TGTCATGGAA TTTCGGGATC ATGTATATGA ACTTCTCAAC 
ACCATTGATG CCTGCCAGTG CCATTTTGAT ATCAATCTCA ACTTTGATTT CACTCGGAGT 
TACCTGGACT TGATTGTAAC TTACACCTCA GTCATTTTAC TTCTGTCACG GATTGAAGAT 
CGGCGGATAC TCATTGGCAT GTACAATTGT GCCCATGAGA TGCTGCATGG GCATGGTGAC 
CCCAGTTTTG CCCGTCTGGG TCAGATGGTC TTGGAGTATG ACCACCCTCT GAAGAAGCTG 
ACAGAAGAGT TTGGGCCTCA CACAAAGGCT GTGAGTGGAG CCCTCCTCTC TTTGCATTTC 
CTCTTTGTCC GAAGAAACCA GGGGGCTGAG CAGTGGCGCA GTGCCCAACT TCTAAGCCTC 
ATCAGCAACC CCCCAGCCAT GATTAACCCT GCTAATTCAG ATACAATGGC CTGTGAGTAT 
CTGTCTGTGG AAGTAATGGA GCGCTGGATT ATCATTGGGT TTCTTCTTTG TCATGGGTGC 
CTCAACTCCA ATAGCCAGTG CCAGAAGCTG TGGAAGCTGT GTCTGCAGGG CTCCCTCTAC 
ATCACCCTTA TCCGTGAGGA TGTGCTGCAG GTGCACAAAG TCACCGAGGA CCTGTTTAGC 
AGTTTGAAAG GGTATGGCAA GAGAGTGGCA GACATAAAGG AGAGCAAGGA ACATGTAATT 
GCAAACAGTG GCCAGTTTCA TTGTCAACGG CGGCAATTTC TGCGGATGGC AGTGAAGGAG 
CTGGAGACTG TGTTGGCTGA TGAACCGGGA CTACTGGGTC CTAAGGCTCT TTTTGCTTTC 
ATGGCCCTGT CCTTCATTCG TGATGAGGTC ACCTGGCTGG TTCGCCACAC AGAGAATGTC 
ACCAAGACAA AGACACCTGA GGACTATGCT GACTCGAGCA TTGCAGAGCT ACTTTTCTTG 
TTGGAGGGGA TTAGGTCTCT GGTCCGAAGA CACATCAAAG TGATACAGCA ATACCACCTT 
CAGTACTTGG CAAGATTTGA TGCTCTTGTG CTCAGTGACA TCATTCAGAA CTTGTCTGTG 
TGTCCAGAGG AGGAGTCCAT CATCATGTCC TCATTCGTCA GTATCCTCTC CTCTCTGAAT 
CTCAAACAAG TTGATAATGG AGAAAAATTT GAATTCTCAG GATTGAGGCT GGACTGGTTC 
CGCCTACAGG CATACACTAG CGTGGCTAAG GCCCCTCTGC ACCTGCATGA GAACCCTGAC 
TTAGCCAAGG TGATGAACCT CATTGTCTTC CACTCCCGAA TGCTGGACTC CGTAGAAAAA 
TTGCTGGTGG AAACTTCTGA TCTGTCTACT TTCTGCTTTC ATCTTCGTAT CTTTGAGAAG 
ATGTTTGCCA TGACCTTGGA GGAATCTGCC ATGTTGCGTT ATGCCATTGC TTTCCCCCTG 
ATTTGTGCTC ACTTTGTCCA CTGCACTCAT GAGATGTGCC CAGAGGAGTA CCCCCACCTC 
AAGAACCATG GTCTTCACCA CTGCAACTCC TTCCTGGAAG AGTTGGCCAA GCAGACCAGC 
AATTGCGTCC TGGAGATCTG TGCTGAGCAG CGAAACCTGA GCGAGCAGCT TCTACCTAAG 
CACTGTGCCA CTACAATCAG CAAAGCCAAG AACAAGAAAA CCAGGAAGCA GAGGCAGACT 
CCCAGAAAAG GAGAGCCCGA GAGGGACAAG CCAGGAGCTG AGAGTCACCG GAAGAACCGC 
AGCATTGTCA CCAACATGGA CAAGCTACAC CTAAACTTGA CAGAACTGGC ACTGACAATG 
AATCATGTAT ACAGTTTCTC CGTGTTTGAA CATACTATCT TCCCTTCTGA GTACCTCAGC 
AGCCACCTGG AGGCCAGACT CAACAGAGCC ATTGTGTGGC TGGCTGGCTA CAATGCCACG 
ACCCAGGAGA TCGTACGGCC TTCTGAGCTG TTGGCAGGAG TCAAAGCATA CATTGGTTTC 
ATACAGTCAC TGGCCCAGTT TTTGGGTGCA GATGCTTCCA GAGTCATCCG CAACGCCCTC 
CTGCAGCAGA CACAACCACT GGATTCCTGT GGGGAACAGA CAATCACCAC ACTCTACACA 
AACTGGTACC TGGAAAGTCT GCTTAGACAG GCAAGCAGTG GGACCATCAT CCTCTCCCCA 
GCCATGCAGG CCTTCGTCAG CCTGCCCAGA GAAGGGGAGC AGAACTTCAG TGCAGAGGAG 
TTCTCTGACA TCTCTGAGAT GCGGGCCTTG GCAGAACTCC TGGGCCCCTA TGGCATGAAG 
TTCCTGAGTG AAAACCTGAT GTGGCATGTG ACCTCTCAGA TTGTGGAGCT GAAGAAGCTG 
GTGGTGGAAA ACATGGACAT ACTTGTTCAG ATCAGATCCA ACTTTAGCAA GCCGGACTTG 
ATGGCTTCCC TGCTGCCCCA GCTGACAGGG GCTGAAAATG TGCTAAAGCG CATGACCATC 
ATTGGGGTTA TCCTCAGTTT CAGGGCCATG GCCCAAGAGG GACTTCGGGA GGTTTTCTCC 
TCCCACTGCC CATTTCTTAT GGGTCCCATT GAGTGCTTGA AGGAGTTTGT CACTCCAGAC 
ACAGACATCA AGGTGACCTT GAGTATCTTT GAGCTGGCAT CTGCTGCAGG TGTGGGCTGT 
GACATTGACC CAGCCTTGGT GGCTGCCATT GCTAATCTGA AAGCTGATAC TTCATCTCCT 
GAGGAGGAAT ATAAGGTGGC CTGCCTGCTC TTGATCTTTC TGGCAGTTTC CCTCCCACTC 
CTTGCCACTG ACCCTTCTTC CTTTTATAGC ATTGAGAAGG ATGGTTACAA CAACAATATT 
CATTGCTTGA CCAAAGCCAT CATCCAGGTG TCTGCTGCCC TCTTCACGCT CTACAACAAG 
AACATTGAAA CTCACCTCAA GGAATTTCTG GTGGTGGCCT CTGTCAGCCT CTTGCAGCTG 
GGCCAGGAGA CTGACAAGCT TAAAACCAGA AATCGAGAAT CCATTTCTCT GCTCATGCGC 
TTGGTGGTGG AGGAGTCATC CTTCCTGACC CTGGACATGC TGGAGTCCTG TTTCCCTTAT 
GTCCTGCTTC GAAATGCCTA TCGGGAGGTG TCTCGGGCCT TCCACCTAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171905.1
CDS	101..3334
Translation	MEPSLKYINKKFPNIDVRNSTQHLGPVHREKAEIIRFLTNYYQSFVDVMEFRDHVYELLNTIDACQCHFDINLNFDFTRSYLDLIVTYTSVILLLSRIEDRRILIGMYNCAHEMLHGHGDPSFARLGQMVLEYDHPLKKLTEEFGPHTKAVSGALLSLHFLFVRRNQGAEQWRSAQLLSLISNPPAMINPANSDTMACEYLSVEVMERWIIIGFLLCHGCLNSNSQCQKLWKLCLQGSLYITLIREDVLQVHKVTEDLFSSLKGYGKRVADIKESKEHVIANSGQFHCQRRQFLRMAVKELETVLADEPGLLGPKALFAFMALSFIRDEVTWLVRHTENVTKTKTPEDYADSSIAELLFLLEGIRSLVRRHIKVIQQYHLQYLARFDALVLSDIIQNLSVCPEEESIIMSSFVSILSSLNLKQVDNGEKFEFSGLRLDWFRLQAYTSVAKAPLHLHENPDLAKVMNLIVFHSRMLDSVEKLLVETSDLSTFCFHLRIFEKMFAMTLEESAMLRYAIAFPLICAHFVHCTHEMCPEEYPHLKNHGLHHCNSFLEELAKQTSNCVLEICAEQRNLSEQLLPKHCATTISKAKNKKTRKQRQTPRKGEPERDKPGAESHRKNRSIVTNMDKLHLNLTELALTMNHVYSFSVFEHTIFPSEYLSSHLEARLNRAIVWLAGYNATTQEIVRPSELLAGVKAYIGFIQSLAQFLGADASRVIRNALLQQTQPLDSCGEQTITTLYTNWYLESLLRQASSGTIILSPAMQAFVSLPREGEQNFSAEEFSDISEMRALAELLGPYGMKFLSENLMWHVTSQIVELKKLVVENMDILVQIRSNFSKPDLMASLLPQLTGAENVLKRMTIIGVILSFRAMAQEGLREVFSSHCPFLMGPIECLKEFVTPDTDIKVTLSIFELASAAGVGCDIDPALVAAIANLKADTSSPEEEYKVACLLLIFLAVSLPLLATDPSSFYSIEKDGYNNNIHCLTKAIIQVSAALFTLYNKNIETHLKEFLVVASVSLLQLGQETDKLKTRNRESISLLMRLVVEESSFLTLDMLESCFPYVLLRNAYREVSRAFHLN

Target ORF information:

RefSeq Version	NM_001184976.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NCK associated protein 1 like (NCKAP1L), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184976.1

ORF Insert Sequence:

ATGGAACCAT CTCTCAAGTA TATCAACAAG AAATTTCCCA ACATAGATGT CCGAAACAGC 
ACGCAACATT TAGGACCAGT ACATCGTGAA AAAGCCGAGA TAATTAGATT CCTCACCAAC 
TACTACCAGT CATTTGTGGA TGTCATGGAA TTTCGGGATC ATGTATATGA ACTTCTCAAC 
ACCATTGATG CCTGCCAGTG CCATTTTGAT ATCAATCTCA ACTTTGATTT CACTCGGAGT 
TACCTGGACT TGATTGTAAC TTACACCTCA GTCATTTTAC TTCTGTCACG GATTGAAGAT 
CGGCGGATAC TCATTGGCAT GTACAATTGT GCCCATGAGA TGCTGCATGG GCATGGTGAC 
CCCAGTTTTG CCCGTCTGGG TCAGATGGTC TTGGAGTATG ACCACCCTCT GAAGAAGCTG 
ACAGAAGAGT TTGGGCCTCA CACAAAGGCT GTGAGTGGAG CCCTCCTCTC TTTGCATTTC 
CTCTTTGTCC GAAGAAACCA GGGGGCTGAG CAGTGGCGCA GTGCCCAACT TCTAAGCCTC 
ATCAGCAACC CCCCAGCCAT GATTAACCCT GCTAATTCAG ATACAATGGC CTGTGAGTAT 
CTGTCTGTGG AAGTAATGGA GCGCTGGATT ATCATTGGGT TTCTTCTTTG TCATGGGTGC 
CTCAACTCCA ATAGCCAGTG CCAGAAGCTG TGGAAGCTGT GTCTGCAGGG CTCCCTCTAC 
ATCACCCTTA TCCGTGAGGA TGTGCTGCAG GTGCACAAAG TCACCGAGGA CCTGTTTAGC 
AGTTTGAAAG GGTATGGCAA GAGAGTGGCA GACATAAAGG AGAGCAAGGA ACATGTAATT 
GCAAACAGTG GCCAGTTTCA TTGTCAACGG CGGCAATTTC TGCGGATGGC AGTGAAGGAG 
CTGGAGACTG TGTTGGCTGA TGAACCGGGA CTACTGGGTC CTAAGGCTCT TTTTGCTTTC 
ATGGCCCTGT CCTTCATTCG TGATGAGGTC ACCTGGCTGG TTCGCCACAC AGAGAATGTC 
ACCAAGACAA AGACACCTGA GGACTATGCT GACTCGAGCA TTGCAGAGCT ACTTTTCTTG 
TTGGAGGGGA TTAGGTCTCT GGTCCGAAGA CACATCAAAG TGATACAGCA ATACCACCTT 
CAGTACTTGG CAAGATTTGA TGCTCTTGTG CTCAGTGACA TCATTCAGAA CTTGTCTGTG 
TGTCCAGAGG AGGAGTCCAT CATCATGTCC TCATTCGTCA GTATCCTCTC CTCTCTGAAT 
CTCAAACAAG TTGATAATGG AGAAAAATTT GAATTCTCAG GATTGAGGCT GGACTGGTTC 
CGCCTACAGG CATACACTAG CGTGGCTAAG GCCCCTCTGC ACCTGCATGA GAACCCTGAC 
TTAGCCAAGG TGATGAACCT CATTGTCTTC CACTCCCGAA TGCTGGACTC CGTAGAAAAA 
TTGCTGGTGG AAACTTCTGA TCTGTCTACT TTCTGCTTTC ATCTTCGTAT CTTTGAGAAG 
ATGTTTGCCA TGACCTTGGA GGAATCTGCC ATGTTGCGTT ATGCCATTGC TTTCCCCCTG 
ATTTGTGCTC ACTTTGTCCA CTGCACTCAT GAGATGTGCC CAGAGGAGTA CCCCCACCTC 
AAGAACCATG GTCTTCACCA CTGCAACTCC TTCCTGGAAG AGTTGGCCAA GCAGACCAGC 
AATTGCGTCC TGGAGATCTG TGCTGAGCAG CGAAACCTGA GCGAGCAGCT TCTACCTAAG 
CACTGTGCCA CTACAATCAG CAAAGCCAAG AACAAGAAAA CCAGGAAGCA GAGGCAGACT 
CCCAGAAAAG GAGAGCCCGA GAGGGACAAG CCAGGAGCTG AGAGTCACCG GAAGAACCGC 
AGCATTGTCA CCAACATGGA CAAGCTACAC CTAAACTTGA CAGAACTGGC ACTGACAATG 
AATCATGTAT ACAGTTTCTC CGTGTTTGAA CATACTATCT TCCCTTCTGA GTACCTCAGC 
AGCCACCTGG AGGCCAGACT CAACAGAGCC ATTGTGTGGC TGGCTGGCTA CAATGCCACG 
ACCCAGGAGA TCGTACGGCC TTCTGAGCTG TTGGCAGGAG TCAAAGCATA CATTGGTTTC 
ATACAGTCAC TGGCCCAGTT TTTGGGTGCA GATGCTTCCA GAGTCATCCG CAACGCCCTC 
CTGCAGCAGA CACAACCACT GGATTCCTGT GGGGAACAGA CAATCACCAC ACTCTACACA 
AACTGGTACC TGGAAAGTCT GCTTAGACAG GCAAGCAGTG GGACCATCAT CCTCTCCCCA 
GCCATGCAGG CCTTCGTCAG CCTGCCCAGA GAAGGGGAGC AGAACTTCAG TGCAGAGGAG 
TTCTCTGACA TCTCTGAGAT GCGGGCCTTG GCAGAACTCC TGGGCCCCTA TGGCATGAAG 
TTCCTGAGTG AAAACCTGAT GTGGCATGTG ACCTCTCAGA TTGTGGAGCT GAAGAAGCTG 
GTGGTGGAAA ACATGGACAT ACTTGTTCAG ATCAGATCCA ACTTTAGCAA GCCGGACTTG 
ATGGCTTCCC TGCTGCCCCA GCTGACAGGG GCTGAAAATG TGCTAAAGCG CATGACCATC 
ATTGGGGTTA TCCTCAGTTT CAGGGCCATG GCCCAAGAGG GACTTCGGGA GGTTTTCTCC 
TCCCACTGCC CATTTCTTAT GGGTCCCATT GAGTGCTTGA AGGAGTTTGT CACTCCAGAC 
ACAGACATCA AGGTGACCTT GAGTATCTTT GAGCTGGCAT CTGCTGCAGG TGTGGGCTGT 
GACATTGACC CAGCCTTGGT GGCTGCCATT GCTAATCTGA AAGCTGATAC TTCATCTCCT 
GAGGAGGAAT ATAAGGTGGC CTGCCTGCTC TTGATCTTTC TGGCAGTTTC CCTCCCACTC 
CTTGCCACTG ACCCTTCTTC CTTTTATAGC ATTGAGAAGG ATGGTTACAA CAACAATATT 
CATTGCTTGA CCAAAGCCAT CATCCAGGTG TCTGCTGCCC TCTTCACGCT CTACAACAAG 
AACATTGAAA CTCACCTCAA GGAATTTCTG GTGGTGGCCT CTGTCAGCCT CTTGCAGCTG 
GGCCAGGAGA CTGACAAGCT TAAAACCAGA AATCGAGAAT CCATTTCTCT GCTCATGCGC 
TTGGTGGTGG AGGAGTCATC CTTCCTGACC CTGGACATGC TGGAGTCCTG TTTCCCTTAT 
GTCCTGCTTC GAAATGCCTA TCGGGAGGTG TCTCGGGCCT TCCACCTAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
PloS one7(12)e51954(2012)
Comuzzie AG,Cole SA,Laston SL,Voruganti VS,Haack K,Gibbs RA,Butte NF

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

ATM, CTLA4, MNDA, and HEM1 in high versus low CD38 expressing B-cell chronic lymphocytic leukemia.
Clinical cancer research : an official journal of the American Association for Cancer Research13(18 Pt 1)5295-304(2007 Sep)
Joshi AD,Hegde GV,Dickinson JD,Mittal AK,Lynch JC,Eudy JD,Armitage JO,Bierman PJ,Bociek RG,Devetten MP,Vose JM,Joshi SS

An actin-based wave generator organizes cell motility.
PLoS biology5(9)e221(2007 Sep)
Weiner OD,Marganski WA,Wu LF,Altschuler SJ,Kirschner MW

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NCKAP1L cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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