HADHA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HADHA
Entrez Gene ID	3030
Full Name	hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha
Synonyms	ECHA,GBP,HADH,LCEH,LCHAD,MTPA,TP-ALPHA
General protein information	Preferred Names hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha Names trifunctional enzyme subunit alpha, mitochondrial 3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit 3-oxoacyl-CoA thiolase 78 kDa gastrin-binding protein gastrin-binding protein hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit long-chain 2-enoyl-CoA hydratase long-chain-3-hydroxyacyl-CoA dehydrogenase mitochondrial long-chain 2-enoyl-Coenzyme A (CoA) hydratase, alpha subunit mitochondrial long-chain L-3-hydroxyacyl-Coenzyme A (CoA) dehydrogenase, alpha subunit mitochondrial trifunctional enzyme, alpha subunit mitochondrial trifunctional protein, alpha subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p23.3
Summary	This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. [provided by RefSeq, Jul 2008].
Disorder MIM:	600890
Disorder Html:	LCHAD deficiency, 609016 (3); Trifunctional protein deficiency, 609015 (3); HELLP syndrome, maternal, of pregnancy, 609016 (3); Fatty liver, acute, of pregnancy, 609016 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000182.4	NP_000173.2	trifunctional enzyme subunit alpha, mitochondrial precursor
NM_000182.5	NP_000173.2	trifunctional enzyme subunit alpha, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	HADHA	NP_000173.2
	HADHA	XP_001086472.1
Canis lupus familiaris (dog)	HADHA	XP_003639634.1
Gallus gallus (chicken)	HADHA	NP_990387.1
Danio rerio (zebrafish)	hadhab	NP_001082906.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007784	XP_317731.4
Pan troglodytes (chimpanzee)	HADHA	XP_515339.2
Mus musculus (house mouse)	Hadha	NP_849209.1
Rattus norvegicus (Norway rat)	Hadha	NP_570839.2
Danio rerio (zebrafish)	hadhaa	NP_001098746.1
Caenorhabditis elegans (roundworm)	T08B2.7	NP_491789.1
Caenorhabditis elegans (roundworm)	ech-1	NP_506810.1
Bos taurus (cattle)	HADHA	NP_776760.1
Drosophila melanogaster (fruit fly)	Mtpalpha	NP_609299.1
Xenopus tropicalis (tropical clawed frog)	hadha	NP_989224.1

Gene Ontology
Bibliography

Related articles in PubMed

High prevalence of carriers of variant c.1528G>C of HADHA gene causing long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency (LCHADD) in the population of adult Kashubians from North Poland.
Nedoszytko B, Siemi?ska A, Strapagiel D, D?browski S, S?omka M, Sobalska-Kwapis M, Marciniak B, Wierzba J, Skokowski J, Fija?kowski M, Nowicki R, Kalinowski L
PloS one12(11)e0187365(2017)

Prognostic significance of two lipid metabolism enzymes, HADHA and ACAT2, in clear cell renal cell carcinoma.
Zhao Z, Lu J, Han L, Wang X, Man Q, Liu S
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(6)8121-30(2016 Jun)

Mitochondrial trifunctional protein deficiency in human cultured fibroblasts: effects of bezafibrate.
Djouadi F, Habarou F, Le Bachelier C, Ferdinandusse S, Schlemmer D, Benoist JF, Boutron A, Andresen BS, Visser G, de Lonlay P, Olpin S, Fukao T, Yamaguchi S, Strauss AW, Wanders RJ, Bastin J
Journal of inherited metabolic disease39(1)47-58(2016 Jan)

Association of HADHA with human RNA silencing machinery.
Kakumani PK, Shanmugam RK, Kaur I, Malhotra P, Mukherjee SK, Bhatnagar RK
Biochemical and biophysical research communications466(3)481-5(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study indicate described high frequency of c.1528G>C variant of HADHA gene in Kashubian population, suggesting the founder effect. For the first time we have found high frequency of rs71441018 in the South Poland Silesian population
Title: High prevalence of carriers of variant c.1528G>C of HADHA gene causing long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency (LCHADD) in the population of adult Kashubians from North Poland.

Low HADHA expression is associated with clear cell renal cell carcinoma.
Title: Prognostic significance of two lipid metabolism enzymes, HADHA and ACAT2, in clear cell renal cell carcinoma.

Exposure to bezafibrate (400 muM for 48 h) increased the abundance of HADHA and HADHB mRNAs.
Title: Mitochondrial trifunctional protein deficiency in human cultured fibroblasts: effects of bezafibrate.

findings suggest an auxiliary role for HADHA in miRNA biogenesis and help in better understanding of molecular mechanisms underlying RNAi pathway
Title: Association of HADHA with human RNA silencing machinery.

nonstructural protein 5 (NS5) interacted with hydroxyacyl-CoA dehydrogenase alpha and beta subunits, two components of the mitochondrial trifunctional protein (MTP) involved in LCFA beta-oxidation
Title: Japanese encephalitis virus nonstructural protein NS5 interacts with mitochondrial trifunctional protein and impairs fatty acid ?-oxidation.

The following HADHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HADHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18150	NM_000182.5 Latest version!	Homo sapiens hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha (HADHA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu18150	NM_000182.4	Homo sapiens hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha (HADHA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HADHA ( NM_000182.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000173.2 Homo sapiens hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha (HADHA), mRNA.

Price & Availability↑

CloneID	OHu18150
Clone ID Related Accession (Same CDS sequence)	NM_000182.4 , NM_000182.5
Accession Version	NM_000182.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	trifunctional enzyme subunit alpha, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN264228.1, BC009235.2, AC011742.3 and AI972144.1. This sequence is a reference standard in the RefSeqGene project. On Jun 1, 2006 this sequence version replaced NM_000182.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009235.2, GU727642.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGGCCT GCCGGGCGAT TGGCATCCTC AGCCGCTTTT CTGCCTTCAG GATCCTCCGC 
TCCCGAGGTT ATATATGCCG CAATTTTACA GGGTCTTCTG CTTTGCTGAC CAGAACCCAT 
ATTAACTATG GAGTCAAAGG GGATGTGGCA GTTGTTCGAA TTAACTCTCC CAATTCAAAG 
GTAAATACAC TGAGTAAAGA GCTACATTCA GAGTTCTCAG AAGTTATGAA TGAAATCTGG 
GCTAGTGATC AAATCAGAAG TGCCGTCCTT ATCTCATCAA AGCCAGGCTG CTTTATTGCA 
GGTGCTGATA TCAACATGTT AGCCGCTTGC AAGACCCTTC AAGAAGTAAC ACAGCTATCA 
CAAGAAGCAC AGAGAATAGT TGAGAAACTT GAAAAGTCCA CAAAGCCTAT TGTGGCTGCC 
ATCAATGGAT CCTGCCTGGG AGGAGGACTT GAGGTTGCCA TTTCATGCCA ATACAGAATA 
GCAACAAAAG ACAGAAAAAC AGTATTAGGT ACCCCTGAAG TTTTGCTGGG GGCCTTACCA 
GGAGCAGGAG GCACACAAAG GCTGCCCAAA ATGGTGGGTG TGCCTGCTGC TTTGGACATG 
ATGCTGACTG GTAGAAGCAT TCGTGCAGAC AGGGCAAAGA AAATGGGACT GGTTGACCAA 
CTGGTGGAAC CCCTGGGACC AGGACTAAAA CCTCCAGAGG AACGGACAAT AGAATACCTA 
GAAGAAGTTG CAATTACTTT TGCCAAAGGA CTAGCTGATA AGAAGATCTC TCCAAAGAGA 
GACAAGGGAT TGGTGGAAAA ATTGACAGCG TATGCCATGA CTATTCCATT TGTCAGGCAA 
CAGGTTTACA AAAAAGTGGA AGAAAAAGTG CGAAAGCAGA CTAAAGGCCT TTATCCTGCA 
CCTCTGAAAA TAATTGATGT GGTAAAGACT GGAATTGAGC AAGGGAGTGA TGCCGGTTAT 
CTCTGTGAAT CTCAGAAATT TGGAGAGCTT GTAATGACCA AAGAATCAAA GGCCTTGATG 
GGACTCTACC ATGGTCAGGT CCTGTGCAAG AAGAATAAAT TTGGAGCTCC ACAGAAGGAT 
GTTAAGCATC TGGCTATTCT TGGTGCAGGG CTGATGGGAG CAGGCATCGC CCAAGTCTCC 
GTGGATAAGG GGCTAAAGAC TATACTTAAA GATGCCACCC TCACTGCGCT AGACCGAGGA 
CAGCAACAAG TGTTCAAAGG ATTGAATGAC AAAGTGAAGA AGAAAGCTCT AACATCATTT 
GAAAGGGATT CCATCTTCAG CAACTTGACT GGGCAGCTTG ATTACCAAGG TTTTGAAAAG 
GCCGACATGG TGATTGAAGC TGTGTTTGAG GACCTTAGTC TTAAGCACAG AGTGCTAAAG 
GAAGTAGAAG CGGTGATTCC AGATCACTGT ATCTTTGCCA GTAACACATC TGCTCTCCCA 
ATCAGTGAAA TCGCTGCTGT CAGCAAAAGA CCTGAGAAGG TGATTGGCAT GCACTACTTC 
TCTCCCGTGG ACAAGATGCA GCTGCTGGAG ATTATCACGA CCGAGAAAAC TTCCAAAGAC 
ACCAGTGCTT CAGCTGTAGC AGTTGGTCTC AAGCAGGGGA AGGTCATCAT TGTGGTTAAG 
GATGGACCTG GCTTCTATAC TACCAGGTGT CTTGCGCCCA TGATGTCTGA AGTCATCCGA 
ATCCTCCAGG AAGGAGTTGA CCCGAAGAAG CTGGATTCCC TGACCACAAG CTTTGGCTTT 
CCTGTGGGTG CCGCCACACT GGTGGATGAA GTTGGTGTGG ATGTAGCGAA ACATGTGGCG 
GAAGATCTGG GCAAAGTCTT TGGGGAGCGG TTTGGAGGTG GAAACCCAGA ACTGCTGACA 
CAGATGGTGT CCAAGGGCTT CCTAGGTCGT AAATCTGGGA AGGGCTTTTA CATCTATCAG 
GAGGGTGTGA AGAGGAAGGA TTTGAATTCT GACATGGATA GTATTTTAGC GAGTCTGAAG 
CTGCCTCCTA AGTCTGAAGT CTCATCAGAC GAAGACATCC AGTTCCGCCT GGTGACAAGA 
TTTGTGAATG AGGCAGTCAT GTGCCTGCAA GAGGGGATCT TGGCCACACC TGCAGAGGGA 
GACATCGGAG CCGTCTTTGG GCTTGGCTTC CCGCCTTGTC TGGGAGGGCC TTTCCGCTTT 
GTGGATCTGT ATGGCGCCCA GAAGATAGTG GACCGGCTCA AGAAATATGA AGCTGCCTAT 
GGAAAACAGT TCACCCCATG CCAGCTGCTA GCTGACCATG CTAACAGCCC TAACAAGAAG 
TTCTACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000173.2
CDS	131..2422
Translation	MVACRAIGILSRFSAFRILRSRGYICRNFTGSSALLTRTHINYGVKGDVAVVRINSPNSKVNTLSKELHSEFSEVMNEIWASDQIRSAVLISSKPGCFIAGADINMLAACKTLQEVTQLSQEAQRIVEKLEKSTKPIVAAINGSCLGGGLEVAISCQYRIATKDRKTVLGTPEVLLGALPGAGGTQRLPKMVGVPAALDMMLTGRSIRADRAKKMGLVDQLVEPLGPGLKPPEERTIEYLEEVAITFAKGLADKKISPKRDKGLVEKLTAYAMTIPFVRQQVYKKVEEKVRKQTKGLYPAPLKIIDVVKTGIEQGSDAGYLCESQKFGELVMTKESKALMGLYHGQVLCKKNKFGAPQKDVKHLAILGAGLMGAGIAQVSVDKGLKTILKDATLTALDRGQQQVFKGLNDKVKKKALTSFERDSIFSNLTGQLDYQGFEKADMVIEAVFEDLSLKHRVLKEVEAVIPDHCIFASNTSALPISEIAAVSKRPEKVIGMHYFSPVDKMQLLEIITTEKTSKDTSASAVAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDSLTTSFGFPVGAATLVDEVGVDVAKHVAEDLGKVFGERFGGGNPELLTQMVSKGFLGRKSGKGFYIYQEGVKRKDLNSDMDSILASLKLPPKSEVSSDEDIQFRLVTRFVNEAVMCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKIVDRLKKYEAAYGKQFTPCQLLADHANSPNKKFYQ

Target ORF information:

RefSeq Version	NM_000182.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha (HADHA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000182.4

ORF Insert Sequence:

ATGGTGGCCT GCCGGGCGAT TGGCATCCTC AGCCGCTTTT CTGCCTTCAG GATCCTCCGC 
TCCCGAGGTT ATATATGCCG CAATTTTACA GGGTCTTCTG CTTTGCTGAC CAGAACCCAT 
ATTAACTATG GAGTCAAAGG GGATGTGGCA GTTGTTCGAA TTAACTCTCC CAATTCAAAG 
GTAAATACAC TGAGTAAAGA GCTACATTCA GAGTTCTCAG AAGTTATGAA TGAAATCTGG 
GCTAGTGATC AAATCAGAAG TGCCGTCCTT ATCTCATCAA AGCCAGGCTG CTTTATTGCA 
GGTGCTGATA TCAACATGTT AGCCGCTTGC AAGACCCTTC AAGAAGTAAC ACAGCTATCA 
CAAGAAGCAC AGAGAATAGT TGAGAAACTT GAAAAGTCCA CAAAGCCTAT TGTGGCTGCC 
ATCAATGGAT CCTGCCTGGG AGGAGGACTT GAGGTTGCCA TTTCATGCCA ATACAGAATA 
GCAACAAAAG ACAGAAAAAC AGTATTAGGT ACCCCTGAAG TTTTGCTGGG GGCCTTACCA 
GGAGCAGGAG GCACACAAAG GCTGCCCAAA ATGGTGGGTG TGCCTGCTGC TTTGGACATG 
ATGCTGACTG GTAGAAGCAT TCGTGCAGAC AGGGCAAAGA AAATGGGACT GGTTGACCAA 
CTGGTGGAAC CCCTGGGACC AGGACTAAAA CCTCCAGAGG AACGGACAAT AGAATACCTA 
GAAGAAGTTG CAATTACTTT TGCCAAAGGA CTAGCTGATA AGAAGATCTC TCCAAAGAGA 
GACAAGGGAT TGGTGGAAAA ATTGACAGCG TATGCCATGA CTATTCCATT TGTCAGGCAA 
CAGGTTTACA AAAAAGTGGA AGAAAAAGTG CGAAAGCAGA CTAAAGGCCT TTATCCTGCA 
CCTCTGAAAA TAATTGATGT GGTAAAGACT GGAATTGAGC AAGGGAGTGA TGCCGGTTAT 
CTCTGTGAAT CTCAGAAATT TGGAGAGCTT GTAATGACCA AAGAATCAAA GGCCTTGATG 
GGACTCTACC ATGGTCAGGT CCTGTGCAAG AAGAATAAAT TTGGAGCTCC ACAGAAGGAT 
GTTAAGCATC TGGCTATTCT TGGTGCAGGG CTGATGGGAG CAGGCATCGC CCAAGTCTCC 
GTGGATAAGG GGCTAAAGAC TATACTTAAA GATGCCACCC TCACTGCGCT AGACCGAGGA 
CAGCAACAAG TGTTCAAAGG ATTGAATGAC AAAGTGAAGA AGAAAGCTCT AACATCATTT 
GAAAGGGATT CCATCTTCAG CAACTTGACT GGGCAGCTTG ATTACCAAGG TTTTGAAAAG 
GCCGACATGG TGATTGAAGC TGTGTTTGAG GACCTTAGTC TTAAGCACAG AGTGCTAAAG 
GAAGTAGAAG CGGTGATTCC AGATCACTGT ATCTTTGCCA GTAACACATC TGCTCTCCCA 
ATCAGTGAAA TCGCTGCTGT CAGCAAAAGA CCTGAGAAGG TGATTGGCAT GCACTACTTC 
TCTCCCGTGG ACAAGATGCA GCTGCTGGAG ATTATCACGA CCGAGAAAAC TTCCAAAGAC 
ACCAGTGCTT CAGCTGTAGC AGTTGGTCTC AAGCAGGGGA AGGTCATCAT TGTGGTTAAG 
GATGGACCTG GCTTCTATAC TACCAGGTGT CTTGCGCCCA TGATGTCTGA AGTCATCCGA 
ATCCTCCAGG AAGGAGTTGA CCCGAAGAAG CTGGATTCCC TGACCACAAG CTTTGGCTTT 
CCTGTGGGTG CCGCCACACT GGTGGATGAA GTTGGTGTGG ATGTAGCGAA ACATGTGGCG 
GAAGATCTGG GCAAAGTCTT TGGGGAGCGG TTTGGAGGTG GAAACCCAGA ACTGCTGACA 
CAGATGGTGT CCAAGGGCTT CCTAGGTCGT AAATCTGGGA AGGGCTTTTA CATCTATCAG 
GAGGGTGTGA AGAGGAAGGA TTTGAATTCT GACATGGATA GTATTTTAGC GAGTCTGAAG 
CTGCCTCCTA AGTCTGAAGT CTCATCAGAC GAAGACATCC AGTTCCGCCT GGTGACAAGA 
TTTGTGAATG AGGCAGTCAT GTGCCTGCAA GAGGGGATCT TGGCCACACC TGCAGAGGGA 
GACATCGGAG CCGTCTTTGG GCTTGGCTTC CCGCCTTGTC TGGGAGGGCC TTTCCGCTTT 
GTGGATCTGT ATGGCGCCCA GAAGATAGTG GACCGGCTCA AGAAATATGA AGCTGCCTAT 
GGAAAACAGT TCACCCCATG CCAGCTGCTA GCTGACCATG CTAACAGCCC TAACAAGAAG 
TTCTACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HADHA ( NM_000182.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000173.2 Homo sapiens hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha (HADHA), mRNA.

Price & Availability↑

CloneID	OHu18150
Clone ID Related Accession (Same CDS sequence)	NM_000182.4 , NM_000182.5
Accession Version	NM_000182.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	trifunctional enzyme subunit alpha, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN264228.1, BC009235.2, AC011742.3 and AI972144.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000182.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.181687.1, SRR1660807.49153.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000380649.8/ ENSP00000370023.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGGCCT GCCGGGCGAT TGGCATCCTC AGCCGCTTTT CTGCCTTCAG GATCCTCCGC 
TCCCGAGGTT ATATATGCCG CAATTTTACA GGGTCTTCTG CTTTGCTGAC CAGAACCCAT 
ATTAACTATG GAGTCAAAGG GGATGTGGCA GTTGTTCGAA TTAACTCTCC CAATTCAAAG 
GTAAATACAC TGAGTAAAGA GCTACATTCA GAGTTCTCAG AAGTTATGAA TGAAATCTGG 
GCTAGTGATC AAATCAGAAG TGCCGTCCTT ATCTCATCAA AGCCAGGCTG CTTTATTGCA 
GGTGCTGATA TCAACATGTT AGCCGCTTGC AAGACCCTTC AAGAAGTAAC ACAGCTATCA 
CAAGAAGCAC AGAGAATAGT TGAGAAACTT GAAAAGTCCA CAAAGCCTAT TGTGGCTGCC 
ATCAATGGAT CCTGCCTGGG AGGAGGACTT GAGGTTGCCA TTTCATGCCA ATACAGAATA 
GCAACAAAAG ACAGAAAAAC AGTATTAGGT ACCCCTGAAG TTTTGCTGGG GGCCTTACCA 
GGAGCAGGAG GCACACAAAG GCTGCCCAAA ATGGTGGGTG TGCCTGCTGC TTTGGACATG 
ATGCTGACTG GTAGAAGCAT TCGTGCAGAC AGGGCAAAGA AAATGGGACT GGTTGACCAA 
CTGGTGGAAC CCCTGGGACC AGGACTAAAA CCTCCAGAGG AACGGACAAT AGAATACCTA 
GAAGAAGTTG CAATTACTTT TGCCAAAGGA CTAGCTGATA AGAAGATCTC TCCAAAGAGA 
GACAAGGGAT TGGTGGAAAA ATTGACAGCG TATGCCATGA CTATTCCATT TGTCAGGCAA 
CAGGTTTACA AAAAAGTGGA AGAAAAAGTG CGAAAGCAGA CTAAAGGCCT TTATCCTGCA 
CCTCTGAAAA TAATTGATGT GGTAAAGACT GGAATTGAGC AAGGGAGTGA TGCCGGTTAT 
CTCTGTGAAT CTCAGAAATT TGGAGAGCTT GTAATGACCA AAGAATCAAA GGCCTTGATG 
GGACTCTACC ATGGTCAGGT CCTGTGCAAG AAGAATAAAT TTGGAGCTCC ACAGAAGGAT 
GTTAAGCATC TGGCTATTCT TGGTGCAGGG CTGATGGGAG CAGGCATCGC CCAAGTCTCC 
GTGGATAAGG GGCTAAAGAC TATACTTAAA GATGCCACCC TCACTGCGCT AGACCGAGGA 
CAGCAACAAG TGTTCAAAGG ATTGAATGAC AAAGTGAAGA AGAAAGCTCT AACATCATTT 
GAAAGGGATT CCATCTTCAG CAACTTGACT GGGCAGCTTG ATTACCAAGG TTTTGAAAAG 
GCCGACATGG TGATTGAAGC TGTGTTTGAG GACCTTAGTC TTAAGCACAG AGTGCTAAAG 
GAAGTAGAAG CGGTGATTCC AGATCACTGT ATCTTTGCCA GTAACACATC TGCTCTCCCA 
ATCAGTGAAA TCGCTGCTGT CAGCAAAAGA CCTGAGAAGG TGATTGGCAT GCACTACTTC 
TCTCCCGTGG ACAAGATGCA GCTGCTGGAG ATTATCACGA CCGAGAAAAC TTCCAAAGAC 
ACCAGTGCTT CAGCTGTAGC AGTTGGTCTC AAGCAGGGGA AGGTCATCAT TGTGGTTAAG 
GATGGACCTG GCTTCTATAC TACCAGGTGT CTTGCGCCCA TGATGTCTGA AGTCATCCGA 
ATCCTCCAGG AAGGAGTTGA CCCGAAGAAG CTGGATTCCC TGACCACAAG CTTTGGCTTT 
CCTGTGGGTG CCGCCACACT GGTGGATGAA GTTGGTGTGG ATGTAGCGAA ACATGTGGCG 
GAAGATCTGG GCAAAGTCTT TGGGGAGCGG TTTGGAGGTG GAAACCCAGA ACTGCTGACA 
CAGATGGTGT CCAAGGGCTT CCTAGGTCGT AAATCTGGGA AGGGCTTTTA CATCTATCAG 
GAGGGTGTGA AGAGGAAGGA TTTGAATTCT GACATGGATA GTATTTTAGC GAGTCTGAAG 
CTGCCTCCTA AGTCTGAAGT CTCATCAGAC GAAGACATCC AGTTCCGCCT GGTGACAAGA 
TTTGTGAATG AGGCAGTCAT GTGCCTGCAA GAGGGGATCT TGGCCACACC TGCAGAGGGA 
GACATCGGAG CCGTCTTTGG GCTTGGCTTC CCGCCTTGTC TGGGAGGGCC TTTCCGCTTT 
GTGGATCTGT ATGGCGCCCA GAAGATAGTG GACCGGCTCA AGAAATATGA AGCTGCCTAT 
GGAAAACAGT TCACCCCATG CCAGCTGCTA GCTGACCATG CTAACAGCCC TAACAAGAAG 
TTCTACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000173.2
CDS	37..2328
Translation	MVACRAIGILSRFSAFRILRSRGYICRNFTGSSALLTRTHINYGVKGDVAVVRINSPNSKVNTLSKELHSEFSEVMNEIWASDQIRSAVLISSKPGCFIAGADINMLAACKTLQEVTQLSQEAQRIVEKLEKSTKPIVAAINGSCLGGGLEVAISCQYRIATKDRKTVLGTPEVLLGALPGAGGTQRLPKMVGVPAALDMMLTGRSIRADRAKKMGLVDQLVEPLGPGLKPPEERTIEYLEEVAITFAKGLADKKISPKRDKGLVEKLTAYAMTIPFVRQQVYKKVEEKVRKQTKGLYPAPLKIIDVVKTGIEQGSDAGYLCESQKFGELVMTKESKALMGLYHGQVLCKKNKFGAPQKDVKHLAILGAGLMGAGIAQVSVDKGLKTILKDATLTALDRGQQQVFKGLNDKVKKKALTSFERDSIFSNLTGQLDYQGFEKADMVIEAVFEDLSLKHRVLKEVEAVIPDHCIFASNTSALPISEIAAVSKRPEKVIGMHYFSPVDKMQLLEIITTEKTSKDTSASAVAVGLKQGKVIIVVKDGPGFYTTRCLAPMMSEVIRILQEGVDPKKLDSLTTSFGFPVGAATLVDEVGVDVAKHVAEDLGKVFGERFGGGNPELLTQMVSKGFLGRKSGKGFYIYQEGVKRKDLNSDMDSILASLKLPPKSEVSSDEDIQFRLVTRFVNEAVMCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKIVDRLKKYEAAYGKQFTPCQLLADHANSPNKKFYQ

Target ORF information:

RefSeq Version	NM_000182.5
Organism	Homo sapiens(human)
Definition	Homo sapiens hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha (HADHA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000182.5

ORF Insert Sequence:

ATGGTGGCCT GCCGGGCGAT TGGCATCCTC AGCCGCTTTT CTGCCTTCAG GATCCTCCGC 
TCCCGAGGTT ATATATGCCG CAATTTTACA GGGTCTTCTG CTTTGCTGAC CAGAACCCAT 
ATTAACTATG GAGTCAAAGG GGATGTGGCA GTTGTTCGAA TTAACTCTCC CAATTCAAAG 
GTAAATACAC TGAGTAAAGA GCTACATTCA GAGTTCTCAG AAGTTATGAA TGAAATCTGG 
GCTAGTGATC AAATCAGAAG TGCCGTCCTT ATCTCATCAA AGCCAGGCTG CTTTATTGCA 
GGTGCTGATA TCAACATGTT AGCCGCTTGC AAGACCCTTC AAGAAGTAAC ACAGCTATCA 
CAAGAAGCAC AGAGAATAGT TGAGAAACTT GAAAAGTCCA CAAAGCCTAT TGTGGCTGCC 
ATCAATGGAT CCTGCCTGGG AGGAGGACTT GAGGTTGCCA TTTCATGCCA ATACAGAATA 
GCAACAAAAG ACAGAAAAAC AGTATTAGGT ACCCCTGAAG TTTTGCTGGG GGCCTTACCA 
GGAGCAGGAG GCACACAAAG GCTGCCCAAA ATGGTGGGTG TGCCTGCTGC TTTGGACATG 
ATGCTGACTG GTAGAAGCAT TCGTGCAGAC AGGGCAAAGA AAATGGGACT GGTTGACCAA 
CTGGTGGAAC CCCTGGGACC AGGACTAAAA CCTCCAGAGG AACGGACAAT AGAATACCTA 
GAAGAAGTTG CAATTACTTT TGCCAAAGGA CTAGCTGATA AGAAGATCTC TCCAAAGAGA 
GACAAGGGAT TGGTGGAAAA ATTGACAGCG TATGCCATGA CTATTCCATT TGTCAGGCAA 
CAGGTTTACA AAAAAGTGGA AGAAAAAGTG CGAAAGCAGA CTAAAGGCCT TTATCCTGCA 
CCTCTGAAAA TAATTGATGT GGTAAAGACT GGAATTGAGC AAGGGAGTGA TGCCGGTTAT 
CTCTGTGAAT CTCAGAAATT TGGAGAGCTT GTAATGACCA AAGAATCAAA GGCCTTGATG 
GGACTCTACC ATGGTCAGGT CCTGTGCAAG AAGAATAAAT TTGGAGCTCC ACAGAAGGAT 
GTTAAGCATC TGGCTATTCT TGGTGCAGGG CTGATGGGAG CAGGCATCGC CCAAGTCTCC 
GTGGATAAGG GGCTAAAGAC TATACTTAAA GATGCCACCC TCACTGCGCT AGACCGAGGA 
CAGCAACAAG TGTTCAAAGG ATTGAATGAC AAAGTGAAGA AGAAAGCTCT AACATCATTT 
GAAAGGGATT CCATCTTCAG CAACTTGACT GGGCAGCTTG ATTACCAAGG TTTTGAAAAG 
GCCGACATGG TGATTGAAGC TGTGTTTGAG GACCTTAGTC TTAAGCACAG AGTGCTAAAG 
GAAGTAGAAG CGGTGATTCC AGATCACTGT ATCTTTGCCA GTAACACATC TGCTCTCCCA 
ATCAGTGAAA TCGCTGCTGT CAGCAAAAGA CCTGAGAAGG TGATTGGCAT GCACTACTTC 
TCTCCCGTGG ACAAGATGCA GCTGCTGGAG ATTATCACGA CCGAGAAAAC TTCCAAAGAC 
ACCAGTGCTT CAGCTGTAGC AGTTGGTCTC AAGCAGGGGA AGGTCATCAT TGTGGTTAAG 
GATGGACCTG GCTTCTATAC TACCAGGTGT CTTGCGCCCA TGATGTCTGA AGTCATCCGA 
ATCCTCCAGG AAGGAGTTGA CCCGAAGAAG CTGGATTCCC TGACCACAAG CTTTGGCTTT 
CCTGTGGGTG CCGCCACACT GGTGGATGAA GTTGGTGTGG ATGTAGCGAA ACATGTGGCG 
GAAGATCTGG GCAAAGTCTT TGGGGAGCGG TTTGGAGGTG GAAACCCAGA ACTGCTGACA 
CAGATGGTGT CCAAGGGCTT CCTAGGTCGT AAATCTGGGA AGGGCTTTTA CATCTATCAG 
GAGGGTGTGA AGAGGAAGGA TTTGAATTCT GACATGGATA GTATTTTAGC GAGTCTGAAG 
CTGCCTCCTA AGTCTGAAGT CTCATCAGAC GAAGACATCC AGTTCCGCCT GGTGACAAGA 
TTTGTGAATG AGGCAGTCAT GTGCCTGCAA GAGGGGATCT TGGCCACACC TGCAGAGGGA 
GACATCGGAG CCGTCTTTGG GCTTGGCTTC CCGCCTTGTC TGGGAGGGCC TTTCCGCTTT 
GTGGATCTGT ATGGCGCCCA GAAGATAGTG GACCGGCTCA AGAAATATGA AGCTGCCTAT 
GGAAAACAGT TCACCCCATG CCAGCTGCTA GCTGACCATG CTAACAGCCC TAACAAGAAG 
TTCTACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High prevalence of carriers of variant c.1528G>C of HADHA gene causing long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency (LCHADD) in the population of adult Kashubians from North Poland.
PloS one12(11)e0187365(2017)
Nedoszytko B,Siemi?ska A,Strapagiel D,D?browski S,S?omka M,Sobalska-Kwapis M,Marciniak B,Wierzba J,Skokowski J,Fija?kowski M,Nowicki R,Kalinowski L

Prognostic significance of two lipid metabolism enzymes, HADHA and ACAT2, in clear cell renal cell carcinoma.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(6)8121-30(2016 Jun)
Zhao Z,Lu J,Han L,Wang X,Man Q,Liu S

Mitochondrial trifunctional protein deficiency in human cultured fibroblasts: effects of bezafibrate.
Journal of inherited metabolic disease39(1)47-58(2016 Jan)
Djouadi F,Habarou F,Le Bachelier C,Ferdinandusse S,Schlemmer D,Benoist JF,Boutron A,Andresen BS,Visser G,de Lonlay P,Olpin S,Fukao T,Yamaguchi S,Strauss AW,Wanders RJ,Bastin J

Association of HADHA with human RNA silencing machinery.
Biochemical and biophysical research communications466(3)481-5(2015 Oct)
Kakumani PK,Shanmugam RK,Kaur I,Malhotra P,Mukherjee SK,Bhatnagar RK

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HADHA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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