H1-0 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	H1-0
Entrez Gene ID	3005
Full Name	H1 histone family member 0
Synonyms	H10,H1FV
General protein information	Preferred Names H1 histone family member 0 Names histone H1.0 H1.0, H1(0), H1-0 histone H1' histone H1(0)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.1
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family. [provided by RefSeq, Oct 2015].
Disorder MIM:	142708

mRNA and Protein(s)

mRNA	Protein	Name
NM_005318.3	NP_005309.1	histone H1.0
NM_005318.4	NP_005309.1	histone H1.0

Pathways from BioSystems

Homologies

	H1F0	XP_001088913.2
Homo sapiens (human)	H1F0	NP_005309.1
Mus musculus (house mouse)	H1f0	NP_032223.2
Pan troglodytes (chimpanzee)	LOC743278	XP_001147037.2

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of Cellular Dynamics and Chromosomal Binding Site Preference of Linker Histones H1.0 and H1.X.
Okuwaki M, Abe M, Hisaoka M, Nagata K
Molecular and cellular biology36(21)2681-2696(2016 Nov)

The linker histone H1.0 generates epigenetic and functional intratumor heterogeneity.
Torres CM, Biran A, Burney MJ, Patel H, Henser-Brownhill T, Cohen AS, Li Y, Ben-Hamo R, Nye E, Spencer-Dene B, Chakravarty P, Efroni S, Matthews N, Misteli T, Meshorer E, Scaffidi P
Science (New York, N.Y.)353(6307)(2016 09)

Role of H1 linker histones in mammalian development and stem cell differentiation.
Pan C, Fan Y
Biochimica et biophysica acta1859(3)496-509(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

H1.X moves more rapidly than other linker histones in vivo Domain swapping between H1.0 and H1.X suggests that the globular domain (GD) and C-terminal domain (CTD) of H1.X independently contribute to the dynamic behavior of H1.X.
Title: Regulation of Cellular Dynamics and Chromosomal Binding Site Preference of Linker Histones H1.0 and H1.X.

this study shows that dynamic epigenetic states defined by the linker histone H1.0 determine which cells within a tumor can sustain the long-term cancer growth.
Title: The linker histone H1.0 generates epigenetic and functional intratumor heterogeneity.

The N-terminal domain contributes toward the differential chromatin binding affinity, whereas the C-terminal domain contributes toward distinct nucleosomal interface of isotypes H10 and H1c.
Title: N- and C-terminal domains determine differential nucleosomal binding geometry and affinity of linker histone isotypes H1(0) and H1c.

Meta-analysis of gene-disease association. (HuGE Navigator)
Title: Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot".

H1(0)histone may be an important factor in normal DC differentiation. Tumor-derived factors may inhibit DC differentiation by affecting H1(0) expression.
Title: H1(0) histone and differentiation of dendritic cells. A molecular target for tumor-derived factors.

The following H1-0 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H1-0 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16206	NM_005318.4 Latest version!	Homo sapiens H1.0 linker histone (H1-0), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu16206	NM_005318.3	Homo sapiens H1 histone family member 0 (H1F0), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H1-0 ( NM_005318.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005309.1 Homo sapiens H1 histone family member 0 (H1F0), mRNA.

Price & Availability↑

CloneID	OHu16206
Clone ID Related Accession (Same CDS sequence)	NM_005318.3 , NM_005318.4
Accession Version	NM_005318.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	histone H1.0
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI462123.1, BX490432.1, BC000145.1, Z97630.11, BC029046.1 and AA206831.1. On Jan 27, 2006 this sequence version replaced NM_005318.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-independent histone :: PMID: 7374750 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCGAGA ATTCCACGTC CGCCCCTGCG GCCAAGCCCA AGCGGGCCAA GGCCTCCAAG 
AAGTCCACAG ACCACCCCAA GTATTCAGAC ATGATCGTGG CTGCCATCCA GGCCGAGAAG 
AACCGCGCTG GCTCCTCGCG CCAGTCCATT CAGAAGTATA TCAAGAGCCA CTACAAGGTG 
GGTGAGAACG CTGACTCGCA GATCAAGTTG TCCATCAAGC GCCTGGTCAC CACCGGTGTC 
CTCAAGCAGA CCAAAGGGGT GGGGGCCTCG GGGTCCTTCC GGCTAGCCAA GAGCGACGAA 
CCCAAGAAGT CAGTGGCCTT CAAGAAGACC AAGAAGGAAA TCAAGAAGGT AGCCACGCCA 
AAGAAGGCAT CCAAGCCCAA GAAGGCTGCC TCCAAAGCCC CAACCAAGAA ACCCAAAGCC 
ACCCCGGTCA AGAAGGCCAA GAAGAAGCTG GCTGCCACGC CCAAGAAAGC CAAAAAACCC 
AAGACTGTCA AAGCCAAGCC GGTCAAGGCA TCCAAGCCCA AAAAGGCCAA ACCAGTGAAA 
CCCAAAGCAA AGTCCAGTGC CAAGAGGGCC GGCAAGAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005309.1
CDS	439..1023
Translation	MTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFRLAKSDEPKKSVAFKKTKKEIKKVATPKKASKPKKAASKAPTKKPKATPVKKAKKKLAATPKKAKKPKTVKAKPVKASKPKKAKPVKPKAKSSAKRAGKKK

Target ORF information:

RefSeq Version	NM_005318.3
Organism	Homo sapiens(human)
Definition	Homo sapiens H1 histone family member 0 (H1F0), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005318.3

ORF Insert Sequence:

ATGACCGAGA ATTCCACGTC CGCCCCTGCG GCCAAGCCCA AGCGGGCCAA GGCCTCCAAG 
AAGTCCACAG ACCACCCCAA GTATTCAGAC ATGATCGTGG CTGCCATCCA GGCCGAGAAG 
AACCGCGCTG GCTCCTCGCG CCAGTCCATT CAGAAGTATA TCAAGAGCCA CTACAAGGTG 
GGTGAGAACG CTGACTCGCA GATCAAGTTG TCCATCAAGC GCCTGGTCAC CACCGGTGTC 
CTCAAGCAGA CCAAAGGGGT GGGGGCCTCG GGGTCCTTCC GGCTAGCCAA GAGCGACGAA 
CCCAAGAAGT CAGTGGCCTT CAAGAAGACC AAGAAGGAAA TCAAGAAGGT AGCCACGCCA 
AAGAAGGCAT CCAAGCCCAA GAAGGCTGCC TCCAAAGCCC CAACCAAGAA ACCCAAAGCC 
ACCCCGGTCA AGAAGGCCAA GAAGAAGCTG GCTGCCACGC CCAAGAAAGC CAAAAAACCC 
AAGACTGTCA AAGCCAAGCC GGTCAAGGCA TCCAAGCCCA AAAAGGCCAA ACCAGTGAAA 
CCCAAAGCAA AGTCCAGTGC CAAGAGGGCC GGCAAGAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H1-0 ( NM_005318.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005309.1 Homo sapiens H1.0 linker histone (H1-0), mRNA.

Price & Availability↑

CloneID	OHu16206
Clone ID Related Accession (Same CDS sequence)	NM_005318.3 , NM_005318.4
Accession Version	NM_005318.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	histone H1.0
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z97630.11. On Dec 3, 2019 this sequence version replaced NM_005318.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC029046.1, SRR1803611.99720.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-independent histone :: PMID: 7374750 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCGAGA ATTCCACGTC CGCCCCTGCG GCCAAGCCCA AGCGGGCCAA GGCCTCCAAG 
AAGTCCACAG ACCACCCCAA GTATTCAGAC ATGATCGTGG CTGCCATCCA GGCCGAGAAG 
AACCGCGCTG GCTCCTCGCG CCAGTCCATT CAGAAGTATA TCAAGAGCCA CTACAAGGTG 
GGTGAGAACG CTGACTCGCA GATCAAGTTG TCCATCAAGC GCCTGGTCAC CACCGGTGTC 
CTCAAGCAGA CCAAAGGGGT GGGGGCCTCG GGGTCCTTCC GGCTAGCCAA GAGCGACGAA 
CCCAAGAAGT CAGTGGCCTT CAAGAAGACC AAGAAGGAAA TCAAGAAGGT AGCCACGCCA 
AAGAAGGCAT CCAAGCCCAA GAAGGCTGCC TCCAAAGCCC CAACCAAGAA ACCCAAAGCC 
ACCCCGGTCA AGAAGGCCAA GAAGAAGCTG GCTGCCACGC CCAAGAAAGC CAAAAAACCC 
AAGACTGTCA AAGCCAAGCC GGTCAAGGCA TCCAAGCCCA AAAAGGCCAA ACCAGTGAAA 
CCCAAAGCAA AGTCCAGTGC CAAGAGGGCC GGCAAGAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005309.1
CDS	317..901
Translation	MTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFRLAKSDEPKKSVAFKKTKKEIKKVATPKKASKPKKAASKAPTKKPKATPVKKAKKKLAATPKKAKKPKTVKAKPVKASKPKKAKPVKPKAKSSAKRAGKKK

Target ORF information:

RefSeq Version	NM_005318.4
Organism	Homo sapiens(human)
Definition	Homo sapiens H1.0 linker histone (H1-0), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005318.4

ORF Insert Sequence:

ATGACCGAGA ATTCCACGTC CGCCCCTGCG GCCAAGCCCA AGCGGGCCAA GGCCTCCAAG 
AAGTCCACAG ACCACCCCAA GTATTCAGAC ATGATCGTGG CTGCCATCCA GGCCGAGAAG 
AACCGCGCTG GCTCCTCGCG CCAGTCCATT CAGAAGTATA TCAAGAGCCA CTACAAGGTG 
GGTGAGAACG CTGACTCGCA GATCAAGTTG TCCATCAAGC GCCTGGTCAC CACCGGTGTC 
CTCAAGCAGA CCAAAGGGGT GGGGGCCTCG GGGTCCTTCC GGCTAGCCAA GAGCGACGAA 
CCCAAGAAGT CAGTGGCCTT CAAGAAGACC AAGAAGGAAA TCAAGAAGGT AGCCACGCCA 
AAGAAGGCAT CCAAGCCCAA GAAGGCTGCC TCCAAAGCCC CAACCAAGAA ACCCAAAGCC 
ACCCCGGTCA AGAAGGCCAA GAAGAAGCTG GCTGCCACGC CCAAGAAAGC CAAAAAACCC 
AAGACTGTCA AAGCCAAGCC GGTCAAGGCA TCCAAGCCCA AAAAGGCCAA ACCAGTGAAA 
CCCAAAGCAA AGTCCAGTGC CAAGAGGGCC GGCAAGAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of Cellular Dynamics and Chromosomal Binding Site Preference of Linker Histones H1.0 and H1.X.
Molecular and cellular biology36(21)2681-2696(2016 Nov)
Okuwaki M,Abe M,Hisaoka M,Nagata K

The linker histone H1.0 generates epigenetic and functional intratumor heterogeneity.
Science (New York, N.Y.)353(6307)(2016 09)
Torres CM,Biran A,Burney MJ,Patel H,Henser-Brownhill T,Cohen AS,Li Y,Ben-Hamo R,Nye E,Spencer-Dene B,Chakravarty P,Efroni S,Matthews N,Misteli T,Meshorer E,Scaffidi P

Role of H1 linker histones in mammalian development and stem cell differentiation.
Biochimica et biophysica acta1859(3)496-509(2016 Mar)
Pan C,Fan Y