MDFIC cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MDFIC
Entrez Gene ID	29969
Full Name	MyoD family inhibitor domain containing
Synonyms	HIC,MDFIC1
General protein information	Preferred Names MyoD family inhibitor domain containing Names myoD family inhibitor domain-containing protein I-mfa domain-containing protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q31.1-q31.2
Summary	This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009].
Disorder MIM:	614511

mRNA and Protein(s)

mRNA	Protein	Name
NM_199072.4	NP_951038.1	myoD family inhibitor domain-containing protein isoform p40
NM_001166346.1	NP_001159818.3	myoD family inhibitor domain-containing protein isoform c
NM_001166345.1	NP_001159817.1	myoD family inhibitor domain-containing protein isoform p32

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MDFIC	NP_001094572.1
Danio rerio (zebrafish)	si:ch211-262n1.4	XP_001919127.2
Mus musculus (house mouse)	Mdfic	NP_780297.3
Gallus gallus (chicken)	MDFIC	XP_416018.1
Danio rerio (zebrafish)	si:ch73-156e19.1	XP_003198654.1
Rattus norvegicus (Norway rat)	Mdfic	NP_001099138.1
Homo sapiens (human)	MDFIC	NP_001159817.1
Pan troglodytes (chimpanzee)	MDFIC	XP_001137214.2
Canis lupus familiaris (dog)	LOC612042	XP_005628805.1
Xenopus tropicalis (tropical clawed frog)	mdfic	XP_002932863.1

Gene Ontology
Bibliography

Related articles in PubMed

Cross-talk between the glucocorticoid receptor and MyoD family inhibitor domain-containing protein provides a new mechanism for generating tissue-specific responses to glucocorticoids.
Oakley RH, Busillo JM, Cidlowski JA
The Journal of biological chemistry292(14)5825-5844(2017 04)

I-mfa domain proteins specifically interact with HTLV-1 Tax and repress its transactivating functions.
Kusano S, Yoshimitsu M, Hachiman M, Ikeda M
Virology486219-27(2015 Dec)

Combined linkage and association analyses identify a novel locus for obesity near PROX1 in Asians.
Kim HJ, Yoo YJ, Ju YS, Lee S, Cho SI, Sung J, Kim JI, Seo JS
Obesity (Silver Spring, Md.)21(11)2405-12(2013 Nov)

Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: novel insights into the regulation of Rev nuclear import.
Gu L, Tsuji T, Jarboui MA, Yeo GP, Sheehy N, Hall WW, Gautier VW
Retrovirology817(2011 Mar)

The complex regulation of HIC (Human I-mfa domain containing protein) expression.
Reiss-Sklan E, Levitzki A, Naveh-Many T
PloS one4(7)e6152(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GR directly represses the MDFIC gene, revealing a negative feedback loop by which glucocorticoids limit MDFIC activity. These findings identify a new binding partner for cytoplasmic GR that modulates the receptor transcriptome and contributes to the tissue-specific actions of glucocorticoids.
Title: Cross-talk between the glucocorticoid receptor and MyoD family inhibitor domain-containing protein provides a new mechanism for generating tissue-specific responses to glucocorticoids.

HIC specifically interacts with HTLV-1 Tax and negatively regulates Tax transactivational activity by altering its subcellular distribution and stability.
Title: I-mfa domain proteins specifically interact with HTLV-1 Tax and repress its transactivating functions.

We demonstrate that HIC selectively interferes with Rev NLS interaction with importin beta and impedes its nuclear import and function, but does not affect Rev nuclear import mediated by transportin.
Title: Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: novel insights into the regulation of Rev nuclear import.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

These data reveal for the first time that I-mfa domain proteins interact with LANA and negatively regulate LANA-mediated activation of Wnt signaling-dependent transcription by inhibiting the formation of the LANA.GSK-3beta complex.
Title: Human I-mfa domain proteins specifically interact with KSHV LANA and affect its regulation of Wnt signaling-dependent transcription.

The following MDFIC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MDFIC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22386	NM_001166346.1 Latest version!	Homo sapiens MyoD family inhibitor domain containing (MDFIC), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
OHu23119	NM_001166345.1 Latest version!	Homo sapiens MyoD family inhibitor domain containing (MDFIC), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu23159	NM_199072.4 Latest version!	Homo sapiens MyoD family inhibitor domain containing (MDFIC), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MDFIC ( NM_001166346.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159818.3 Homo sapiens MyoD family inhibitor domain containing (MDFIC), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22386
Clone ID Related Accession (Same CDS sequence)	NM_001166346.1
Accession Version	NM_001166346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	myoD family inhibitor domain-containing protein isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF054589.1 and CB048531.1. Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (c) has a distinct and shorter C-terminus, compared to isoform p40. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB048531.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 10671520 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

GTGCGCGGAG TCAGAGCCGC CACCGCTGCC GCAGTTGCCG CCACTGCGGC GTCTGGGCTG 
AGCCGGAGGG AGGCGGGAGG ACGCGCAGGG GCGGCCGCCG CCGTCGTCAG GCCACCGGGG 
CGAAAATGCG GCCGCTGCCG GAGGCTCGCT AACTTTCCGG GGCGGAAGAG GAGGAGGAGG 
AGGAGGAAGG GGCTTGGAGC GACTACGGGG GGATGCGGAG AAGCAGTCAG TTCCCTGCAC 
CCAGCACCTC ACAGCCCTTC CTCCGTGCGC CCTGCCGGGC GGCGAGCTAG GCGGCAGCGG 
CGCGGCGCGG GCTCGGCGGA GCGGCCCATG TCCGGCGCGG GCGAAGCCCT CGCTCCCGGG 
CCCGTGGGGC CGCAGCGCGT GGCCGAGGCG GGCGGCGGCC AGCTGGGCTC CACAGCCCAG 
GGTCTCTTCA GCAGATCCAG GACTGCCGCA GCTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159818.3
CDS	264..722
Translation	VRGVRAATAAAVAATAASGLSRREAGGRAGAAAAVVRPPGRKCGRCRRLANFPGRKRRRRRRKGLGATTGGCGEAVSSLHPAPHSPSSVRPAGRRARRQRRGAGSAERPMSGAGEALAPGPVGPQRVAEAGGGQLGSTAQGLFSRSRTAAAL

Target ORF information:

RefSeq Version	NM_001166346.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MyoD family inhibitor domain containing (MDFIC), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166346.1

ORF Insert Sequence:

GTGCGCGGAG TCAGAGCCGC CACCGCTGCC GCAGTTGCCG CCACTGCGGC GTCTGGGCTG 
AGCCGGAGGG AGGCGGGAGG ACGCGCAGGG GCGGCCGCCG CCGTCGTCAG GCCACCGGGG 
CGAAAATGCG GCCGCTGCCG GAGGCTCGCT AACTTTCCGG GGCGGAAGAG GAGGAGGAGG 
AGGAGGAAGG GGCTTGGAGC GACTACGGGG GGATGCGGAG AAGCAGTCAG TTCCCTGCAC 
CCAGCACCTC ACAGCCCTTC CTCCGTGCGC CCTGCCGGGC GGCGAGCTAG GCGGCAGCGG 
CGCGGCGCGG GCTCGGCGGA GCGGCCCATG TCCGGCGCGG GCGAAGCCCT CGCTCCCGGG 
CCCGTGGGGC CGCAGCGCGT GGCCGAGGCG GGCGGCGGCC AGCTGGGCTC CACAGCCCAG 
GGTCTCTTCA GCAGATCCAG GACTGCCGCA GCTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDFIC ( NM_001166345.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159817.1 Homo sapiens MyoD family inhibitor domain containing (MDFIC), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23119
Clone ID Related Accession (Same CDS sequence)	NM_001166345.1
Accession Version	NM_001166345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	myoD family inhibitor domain-containing protein isoform p32
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF054589.1, AC017003.4 and AA406528.1. Transcript Variant: This variant (1) represents the longer transcript. It encodes isoform p32 via the use of a downstream AUG translation initiation codon. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF054589.1, ERR279854.2623.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression regulatory uORF :: PMID: 19582149 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCGGCG CGGGCGAAGC CCTCGCTCCC GGGCCCGTGG GGCCGCAGCG CGTGGCCGAG 
GCGGGCGGCG GCCAGCTGGG CTCCACAGCC CAGGGAAAAT GTGATAAAGA CAATACTGAG 
AAAGATATAA CTCAAGCTAC CAATAGCCAC TTCACACATG GAGAGATGCA AGACCAGTCC 
ATTTGGGGAA ATCCTTCGGA TGGTGAACTC ATTAGAACCC AACCTCAGCG CTTGCCTCAG 
CTTCAGACTT CAGCCCAGGT GCCAAGTGGT GAGGAAATAG GCAAGATAAA GAACGGCCAC 
ACAGGTCTGA GCAATGGAAA TGGAATTCAC CACGGGGCCA AACACGGATC CGCAGATAAT 
CGCAAACTTT CAGCACCTGT TTCTCAAAAA ATGCATAGAA AAATTCAGTC CAGCTTGTCT 
GTAAACAGCG ATATCAGTAA GAAGAGCAAA GTAAATGCTG TCTTTTCCCA AAAGACAGGC 
TCTTCACCTG AAGATTGTTG TGTCCACTGT ATCCTGGCTT GCTTGTTCTG CGAATTCCTG 
ACCCTTTGCA ACATTGTCCT GGGACAAGCG TCATGTGGCA TCTGCACCTC AGAAGCCTGC 
TGCTGTTGCT GTGGTGACGA GATGGGGGAT GATTGTAACT GCCCTTGTGA TATGGACTGT 
GGCATCATGG ATGCCTGTTG TGAATCATCA GACTGCTTGG AAATCTGTAT GGAATGCTGT 
GGAATTTGTT TTCCTTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159817.1
CDS	591..1331
Translation	MSGAGEALAPGPVGPQRVAEAGGGQLGSTAQGKCDKDNTEKDITQATNSHFTHGEMQDQSIWGNPSDGELIRTQPQRLPQLQTSAQVPSGEEIGKIKNGHTGLSNGNGIHHGAKHGSADNRKLSAPVSQKMHRKIQSSLSVNSDISKKSKVNAVFSQKTGSSPEDCCVHCILACLFCEFLTLCNIVLGQASCGICTSEACCCCCGDEMGDDCNCPCDMDCGIMDACCESSDCLEICMECCGICFPS

Target ORF information:

RefSeq Version	NM_001166345.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MyoD family inhibitor domain containing (MDFIC), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166345.1

ORF Insert Sequence:

ATGTCCGGCG CGGGCGAAGC CCTCGCTCCC GGGCCCGTGG GGCCGCAGCG CGTGGCCGAG 
GCGGGCGGCG GCCAGCTGGG CTCCACAGCC CAGGGAAAAT GTGATAAAGA CAATACTGAG 
AAAGATATAA CTCAAGCTAC CAATAGCCAC TTCACACATG GAGAGATGCA AGACCAGTCC 
ATTTGGGGAA ATCCTTCGGA TGGTGAACTC ATTAGAACCC AACCTCAGCG CTTGCCTCAG 
CTTCAGACTT CAGCCCAGGT GCCAAGTGGT GAGGAAATAG GCAAGATAAA GAACGGCCAC 
ACAGGTCTGA GCAATGGAAA TGGAATTCAC CACGGGGCCA AACACGGATC CGCAGATAAT 
CGCAAACTTT CAGCACCTGT TTCTCAAAAA ATGCATAGAA AAATTCAGTC CAGCTTGTCT 
GTAAACAGCG ATATCAGTAA GAAGAGCAAA GTAAATGCTG TCTTTTCCCA AAAGACAGGC 
TCTTCACCTG AAGATTGTTG TGTCCACTGT ATCCTGGCTT GCTTGTTCTG CGAATTCCTG 
ACCCTTTGCA ACATTGTCCT GGGACAAGCG TCATGTGGCA TCTGCACCTC AGAAGCCTGC 
TGCTGTTGCT GTGGTGACGA GATGGGGGAT GATTGTAACT GCCCTTGTGA TATGGACTGT 
GGCATCATGG ATGCCTGTTG TGAATCATCA GACTGCTTGG AAATCTGTAT GGAATGCTGT 
GGAATTTGTT TTCCTTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDFIC ( NM_199072.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_951038.1 Homo sapiens MyoD family inhibitor domain containing (MDFIC), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23159
Clone ID Related Accession (Same CDS sequence)	NM_199072.4
Accession Version	NM_199072.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	myoD family inhibitor domain-containing protein isoform p40
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF054589.1, AC017003.4 and AA406528.1. This sequence is a reference standard in the RefSeqGene project. On Oct 20, 2009 this sequence version replaced NM_199072.3. Transcript Variant: This variant (1) represents the longer transcript. It encodes the longest isoform (p40) via the use of an upstream non-AUG (GUG) translation initiation codon. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS ID represents the longest MDFIC isoform, known as p40, as described in PMIDs 11139147 and 10671520. This isoform initiates translation at a non-AUG (GUG) start codon that is well-conserved. Alternative translation initiation at a downstream AUG start codon produces an isoform that is 109 aa shorter at the N-terminus, known as p32. These isoforms exhibit distinct subcellular distributions, as indicated in PMID:10671520. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF054589.1, ERR279854.2623.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 10671520 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

GTGCGCGGAG TCAGAGCCGC CACCGCTGCC GCAGTTGCCG CCACTGCGGC GTCTGGGCTG 
AGCCGGAGGG AGGCGGGAGG ACGCGCAGGG GCGGCCGCCG CCGTCGTCAG GCCACCGGGG 
CGAAAATGCG GCCGCTGCCG GAGGCTCGCT AACTTTCCGG GGCGGAAGAG GAGGAGGAGG 
AGGAGGAAGG GGCTTGGAGC GACTACGGGG GGATGCGGAG AAGCAGTCAG TTCCCTGCAC 
CCAGCACCTC ACAGCCCTTC CTCCGTGCGC CCTGCCGGGC GGCGAGCTAG GCGGCAGCGG 
CGCGGCGCGG GCTCGGCGGA GCGGCCCATG TCCGGCGCGG GCGAAGCCCT CGCTCCCGGG 
CCCGTGGGGC CGCAGCGCGT GGCCGAGGCG GGCGGCGGCC AGCTGGGCTC CACAGCCCAG 
GGAAAATGTG ATAAAGACAA TACTGAGAAA GATATAACTC AAGCTACCAA TAGCCACTTC 
ACACATGGAG AGATGCAAGA CCAGTCCATT TGGGGAAATC CTTCGGATGG TGAACTCATT 
AGAACCCAAC CTCAGCGCTT GCCTCAGCTT CAGACTTCAG CCCAGGTGCC AAGTGGTGAG 
GAAATAGGCA AGATAAAGAA CGGCCACACA GGTCTGAGCA ATGGAAATGG AATTCACCAC 
GGGGCCAAAC ACGGATCCGC AGATAATCGC AAACTTTCAG CACCTGTTTC TCAAAAAATG 
CATAGAAAAA TTCAGTCCAG CTTGTCTGTA AACAGCGATA TCAGTAAGAA GAGCAAAGTA 
AATGCTGTCT TTTCCCAAAA GACAGGCTCT TCACCTGAAG ATTGTTGTGT CCACTGTATC 
CTGGCTTGCT TGTTCTGCGA ATTCCTGACC CTTTGCAACA TTGTCCTGGG ACAAGCGTCA 
TGTGGCATCT GCACCTCAGA AGCCTGCTGC TGTTGCTGTG GTGACGAGAT GGGGGATGAT 
TGTAACTGCC CTTGTGATAT GGACTGTGGC ATCATGGATG CCTGTTGTGA ATCATCAGAC 
TGCTTGGAAA TCTGTATGGA ATGCTGTGGA ATTTGTTTTC CTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_951038.1
CDS	264..1331
Translation	VRGVRAATAAAVAATAASGLSRREAGGRAGAAAAVVRPPGRKCGRCRRLANFPGRKRRRRRRKGLGATTGGCGEAVSSLHPAPHSPSSVRPAGRRARRQRRGAGSAERPMSGAGEALAPGPVGPQRVAEAGGGQLGSTAQGKCDKDNTEKDITQATNSHFTHGEMQDQSIWGNPSDGELIRTQPQRLPQLQTSAQVPSGEEIGKIKNGHTGLSNGNGIHHGAKHGSADNRKLSAPVSQKMHRKIQSSLSVNSDISKKSKVNAVFSQKTGSSPEDCCVHCILACLFCEFLTLCNIVLGQASCGICTSEACCCCCGDEMGDDCNCPCDMDCGIMDACCESSDCLEICMECCGICFPS

Target ORF information:

RefSeq Version	NM_199072.4
Organism	Homo sapiens(human)
Definition	Homo sapiens MyoD family inhibitor domain containing (MDFIC), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199072.4

ORF Insert Sequence:

GTGCGCGGAG TCAGAGCCGC CACCGCTGCC GCAGTTGCCG CCACTGCGGC GTCTGGGCTG 
AGCCGGAGGG AGGCGGGAGG ACGCGCAGGG GCGGCCGCCG CCGTCGTCAG GCCACCGGGG 
CGAAAATGCG GCCGCTGCCG GAGGCTCGCT AACTTTCCGG GGCGGAAGAG GAGGAGGAGG 
AGGAGGAAGG GGCTTGGAGC GACTACGGGG GGATGCGGAG AAGCAGTCAG TTCCCTGCAC 
CCAGCACCTC ACAGCCCTTC CTCCGTGCGC CCTGCCGGGC GGCGAGCTAG GCGGCAGCGG 
CGCGGCGCGG GCTCGGCGGA GCGGCCCATG TCCGGCGCGG GCGAAGCCCT CGCTCCCGGG 
CCCGTGGGGC CGCAGCGCGT GGCCGAGGCG GGCGGCGGCC AGCTGGGCTC CACAGCCCAG 
GGAAAATGTG ATAAAGACAA TACTGAGAAA GATATAACTC AAGCTACCAA TAGCCACTTC 
ACACATGGAG AGATGCAAGA CCAGTCCATT TGGGGAAATC CTTCGGATGG TGAACTCATT 
AGAACCCAAC CTCAGCGCTT GCCTCAGCTT CAGACTTCAG CCCAGGTGCC AAGTGGTGAG 
GAAATAGGCA AGATAAAGAA CGGCCACACA GGTCTGAGCA ATGGAAATGG AATTCACCAC 
GGGGCCAAAC ACGGATCCGC AGATAATCGC AAACTTTCAG CACCTGTTTC TCAAAAAATG 
CATAGAAAAA TTCAGTCCAG CTTGTCTGTA AACAGCGATA TCAGTAAGAA GAGCAAAGTA 
AATGCTGTCT TTTCCCAAAA GACAGGCTCT TCACCTGAAG ATTGTTGTGT CCACTGTATC 
CTGGCTTGCT TGTTCTGCGA ATTCCTGACC CTTTGCAACA TTGTCCTGGG ACAAGCGTCA 
TGTGGCATCT GCACCTCAGA AGCCTGCTGC TGTTGCTGTG GTGACGAGAT GGGGGATGAT 
TGTAACTGCC CTTGTGATAT GGACTGTGGC ATCATGGATG CCTGTTGTGA ATCATCAGAC 
TGCTTGGAAA TCTGTATGGA ATGCTGTGGA ATTTGTTTTC CTTCATAA