MRM2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRM2
Entrez Gene ID	29960
Full Name	mitochondrial rRNA methyltransferase 2
Synonyms	FJH1,FTSJ2,HEL97,RRMJ2
General protein information	Preferred Names mitochondrial rRNA methyltransferase 2 Names rRNA methyltransferase 2, mitochondrial 16S rRNA (uridine(1369)-2'-O)-methyltransferase 16S rRNA [Um1369] 2'-O-methyltransferase FtsJ RNA methyltransferase homolog 2 FtsJ homolog 2 MRM2 RNA methyltransferase homolog cell division protein FtsJ epididymis luminal protein 97 protein ftsJ homolog 2 putative ribosomal RNA methyltransferase 2 rRNA (uridine-2'-O-)-methyltransferase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p22.3
Summary	The protein encoded by this gene is a member of the S-adenosylmethionine-binding protein family. It is a nucleolar protein and it may be involved in the processing and modification of rRNA. This gene has been suggested to be involved in cell cycle control and DNA repair. [provided by RefSeq, Jul 2008].
Disorder MIM:	606906

mRNA and Protein(s)

mRNA	Protein	Name
NM_013393.1	NP_037525.1	rRNA methyltransferase 2, mitochondrial
NM_013393.2	NP_037525.1	rRNA methyltransferase 2, mitochondrial
NM_013393.3	NP_037525.1	rRNA methyltransferase 2, mitochondrial

Pathways from BioSystems

Homologies

Homo sapiens (human)	FTSJ2	NP_037525.1
Danio rerio (zebrafish)	LOC555586	XP_683228.2
Saccharomyces cerevisiae (baker's yeast)	MRM2	NP_011379.1
Pan troglodytes (chimpanzee)	FTSJ2	XP_003318261.1
Bos taurus (cattle)	FTSJ2	NP_001178187.1
Rattus norvegicus (Norway rat)	Ftsj2	NP_001100595.1
Gallus gallus (chicken)	FTSJ2	NP_001264474.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013223	XP_003436733.1
Caenorhabditis elegans (roundworm)	F45G2.9	NP_499776.1
Kluyveromyces lactis	KLLA0B01980g	XP_451619.1
Drosophila melanogaster (fruit fly)	CG11447	NP_650835.1
Eremothecium gossypii	AGOS_AFL117C	NP_985433.2
Magnaporthe oryzae (rice blast fungus)	MGG_04502	XP_003710953.1
Canis lupus familiaris (dog)	FTSJ2	XP_003434808.1
Xenopus tropicalis (tropical clawed frog)	ftsj2	XP_002944869.1
	FTSJ2	XP_001086161.1
Mus musculus (house mouse)	Ftsj2	NP_080786.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004177	XP_313065.4

Gene Ontology
Bibliography

Related articles in PubMed

Defective mitochondrial rRNA methyltransferase MRM2 causes MELAS-like clinical syndrome.
Garone C, D'Souza AR, Dallabona C, Lodi T, Rebelo-Guiomar P, Rorbach J, Donati MA, Procopio E, Montomoli M, Guerrini R, Zeviani M, Calvo SE, Mootha VK, DiMauro S, Ferrero I, Minczuk M
Human molecular genetics26(21)4257-4266(2017 Nov)

MiR-542-3p exerts tumor suppressive functions in non-small cell lung cancer cells by upregulating FTSJ2.
Liu B, Li J, Zheng M, Ge J, Li J, Yu P
Life sciences18887-95(2017 Nov)

MRM2 and MRM3 are involved in biogenesis of the large subunit of the mitochondrial ribosome.
Rorbach J, Boesch P, Gammage PA, Nicholls TJ, Pearce SF, Patel D, Hauser A, Perocchi F, Minczuk M
Molecular biology of the cell25(17)2542-55(2014 Sep)

Assignment of 2'-O-methyltransferases to modification sites on the mammalian mitochondrial large subunit 16 S ribosomal RNA (rRNA).
Lee KW, Bogenhagen DF
The Journal of biological chemistry289(36)24936-42(2014 Sep)

FTSJ2, a heat shock-inducible mitochondrial protein, suppresses cell invasion and migration.
Lai CW, Chen HL, Lin KY, Liu FC, Chong KY, Cheng WT, Chen CM
PloS one9(3)e90818(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-542-3p functions as a suppressor gene by targeting and upregulating FTSJ2, thus inhibiting the malignancy of non-small cell lung cancer cells
Title: MiR-542-3p exerts tumor suppressive functions in non-small cell lung cancer cells by upregulating FTSJ2.

MRM2 and MRM3 are human mitochondrial methyltransferases involved in the modification of 16S rRNA and are important factors for the biogenesis and function of the large subunit of the mitochondrial ribosome.
Title: MRM2 and MRM3 are involved in biogenesis of the large subunit of the mitochondrial ribosome.

Data show that 2'-O-methyltransferases MRM1, MRM2, and RNMTL1 are responsible for modification of large subunit rRNA at residues G1145, U1369, and G1370, respectively.
Title: Assignment of 2'-O-methyltransferases to modification sites on the mammalian mitochondrial large subunit 16 S ribosomal RNA (rRNA).

FTSJ2 possesses suppressive effects on the invasion and migration of cancer cells.
Title: FTSJ2, a heat shock-inducible mitochondrial protein, suppresses cell invasion and migration.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following MRM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17135	NM_013393.3 Latest version!	Homo sapiens mitochondrial rRNA methyltransferase 2 (MRM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu17135	NM_013393.1	Homo sapiens mitochondrial rRNA methyltransferase 2 (MRM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu17135	NM_013393.2	Homo sapiens mitochondrial rRNA methyltransferase 2 (MRM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRM2 ( NM_013393.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_037525.1 Homo sapiens mitochondrial rRNA methyltransferase 2 (MRM2), mRNA.

Price & Availability↑

CloneID	OHu17135
Clone ID Related Accession (Same CDS sequence)	NM_013393.1 , NM_013393.2 , NM_013393.3
Accession Version	NM_013393.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-14
Organism	Homo sapiens(human)
Product	rRNA methyltransferase 2, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF093415.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF093415.1, EU794658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157511 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT ACTTGAAGCT GGTGTGTGTT TCCTTTCAGC GTCAAGGGTT CCACACTGTT 
GGGAGTCGCT GCAAGAATCG GACAGGCGCT GAGCACCTGT GGCTGACCCG ACATCTCAGG 
GACCCATTTG TGAAGGCTGC GAAGGTGGAG AGTTACCGGT GTCGAAGCGC CTTCAAGCTC 
CTGGAGGTGA ACGAGAGGCA CCAGATTCTG CGGCCCGGCC TTCGGGTGTT AGACTGTGGG 
GCAGCTCCTG GGGCCTGGAG TCAGGTGGCG GTGCAGAAGG TCAACGCCGC AGGCACAGAT 
CCCAGCTCTC CTGTTGGCTT CGTGCTTGGG GTAGATCTTC TTCACATATT CCCCCTGGAA 
GGAGCAACTT TTCTGTGCCC TGCTGACGTG ACTGACCCGA GAACCTCACA GAGAATCCTC 
GAGGTGCTTC CTGGCAGGAG AGCAGATGTG ATTCTGAGCG ACATGGCGCC CAATGCCACA 
GGGTTCCGGG ACCTCGATCA TGACAGGCTC ATCAGCCTGT GCCTGACCCT TCTCAGCGTG 
ACCCCAGACA TCCTGCAACC TGGGGGGACA TTCCTTTGTA AAACCTGGGC TGGAAGTCAA 
AGCCGTCGGT TACAGAGGAG ACTGACAGAG GAATTCCAGA ATGTAAGGAT CATCAAACCT 
GAAGCCAGCA GGAAAGAGTC ATCAGAAGTG TACTTCTTGG CCACACAGTA CCACGGAAGG 
AAGGGCACTG TGAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037525.1
CDS	30..770
Translation	MAGYLKLVCVSFQRQGFHTVGSRCKNRTGAEHLWLTRHLRDPFVKAAKVESYRCRSAFKLLEVNERHQILRPGLRVLDCGAAPGAWSQVAVQKVNAAGTDPSSPVGFVLGVDLLHIFPLEGATFLCPADVTDPRTSQRILEVLPGRRADVILSDMAPNATGFRDLDHDRLISLCLTLLSVTPDILQPGGTFLCKTWAGSQSRRLQRRLTEEFQNVRIIKPEASRKESSEVYFLATQYHGRKGTVKQ

Target ORF information:

RefSeq Version	NM_013393.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial rRNA methyltransferase 2 (MRM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013393.1

ORF Insert Sequence:

ATGGCGGGGT ACTTGAAGCT GGTGTGTGTT TCCTTTCAGC GTCAAGGGTT CCACACTGTT 
GGGAGTCGCT GCAAGAATCG GACAGGCGCT GAGCACCTGT GGCTGACCCG ACATCTCAGG 
GACCCATTTG TGAAGGCTGC GAAGGTGGAG AGTTACCGGT GTCGAAGCGC CTTCAAGCTC 
CTGGAGGTGA ACGAGAGGCA CCAGATTCTG CGGCCCGGCC TTCGGGTGTT AGACTGTGGG 
GCAGCTCCTG GGGCCTGGAG TCAGGTGGCG GTGCAGAAGG TCAACGCCGC AGGCACAGAT 
CCCAGCTCTC CTGTTGGCTT CGTGCTTGGG GTAGATCTTC TTCACATATT CCCCCTGGAA 
GGAGCAACTT TTCTGTGCCC TGCTGACGTG ACTGACCCGA GAACCTCACA GAGAATCCTC 
GAGGTGCTTC CTGGCAGGAG AGCAGATGTG ATTCTGAGCG ACATGGCGCC CAATGCCACA 
GGGTTCCGGG ACCTCGATCA TGACAGGCTC ATCAGCCTGT GCCTGACCCT TCTCAGCGTG 
ACCCCAGACA TCCTGCAACC TGGGGGGACA TTCCTTTGTA AAACCTGGGC TGGAAGTCAA 
AGCCGTCGGT TACAGAGGAG ACTGACAGAG GAATTCCAGA ATGTAAGGAT CATCAAACCT 
GAAGCCAGCA GGAAAGAGTC ATCAGAAGTG TACTTCTTGG CCACACAGTA CCACGGAAGG 
AAGGGCACTG TGAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRM2 ( NM_013393.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_037525.1 Homo sapiens mitochondrial rRNA methyltransferase 2 (MRM2), mRNA.

Price & Availability↑

CloneID	OHu17135
Clone ID Related Accession (Same CDS sequence)	NM_013393.1 , NM_013393.2 , NM_013393.3
Accession Version	NM_013393.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-16
Organism	Homo sapiens(human)
Product	rRNA methyltransferase 2, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004971.3, AF093415.1 and AC005282.4. On May 17, 2019 this sequence version replaced NM_013393.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.71247.1, SRR1803611.262640.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157511 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT ACTTGAAGCT GGTGTGTGTT TCCTTTCAGC GTCAAGGGTT CCACACTGTT 
GGGAGTCGCT GCAAGAATCG GACAGGCGCT GAGCACCTGT GGCTGACCCG ACATCTCAGG 
GACCCATTTG TGAAGGCTGC GAAGGTGGAG AGTTACCGGT GTCGAAGCGC CTTCAAGCTC 
CTGGAGGTGA ACGAGAGGCA CCAGATTCTG CGGCCCGGCC TTCGGGTGTT AGACTGTGGG 
GCAGCTCCTG GGGCCTGGAG TCAGGTGGCG GTGCAGAAGG TCAACGCCGC AGGCACAGAT 
CCCAGCTCTC CTGTTGGCTT CGTGCTTGGG GTAGATCTTC TTCACATATT CCCCCTGGAA 
GGAGCAACTT TTCTGTGCCC TGCTGACGTG ACTGACCCGA GAACCTCACA GAGAATCCTC 
GAGGTGCTTC CTGGCAGGAG AGCAGATGTG ATTCTGAGCG ACATGGCGCC CAATGCCACA 
GGGTTCCGGG ACCTCGATCA TGACAGGCTC ATCAGCCTGT GCCTGACCCT TCTCAGCGTG 
ACCCCAGACA TCCTGCAACC TGGGGGGACA TTCCTTTGTA AAACCTGGGC TGGAAGTCAA 
AGCCGTCGGT TACAGAGGAG ACTGACAGAG GAATTCCAGA ATGTAAGGAT CATCAAACCT 
GAAGCCAGCA GGAAAGAGTC ATCAGAAGTG TACTTCTTGG CCACACAGTA CCACGGAAGG 
AAGGGCACTG TGAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037525.1
CDS	37..777
Translation	MAGYLKLVCVSFQRQGFHTVGSRCKNRTGAEHLWLTRHLRDPFVKAAKVESYRCRSAFKLLEVNERHQILRPGLRVLDCGAAPGAWSQVAVQKVNAAGTDPSSPVGFVLGVDLLHIFPLEGATFLCPADVTDPRTSQRILEVLPGRRADVILSDMAPNATGFRDLDHDRLISLCLTLLSVTPDILQPGGTFLCKTWAGSQSRRLQRRLTEEFQNVRIIKPEASRKESSEVYFLATQYHGRKGTVKQ

Target ORF information:

RefSeq Version	NM_013393.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial rRNA methyltransferase 2 (MRM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013393.2

ORF Insert Sequence:

ATGGCGGGGT ACTTGAAGCT GGTGTGTGTT TCCTTTCAGC GTCAAGGGTT CCACACTGTT 
GGGAGTCGCT GCAAGAATCG GACAGGCGCT GAGCACCTGT GGCTGACCCG ACATCTCAGG 
GACCCATTTG TGAAGGCTGC GAAGGTGGAG AGTTACCGGT GTCGAAGCGC CTTCAAGCTC 
CTGGAGGTGA ACGAGAGGCA CCAGATTCTG CGGCCCGGCC TTCGGGTGTT AGACTGTGGG 
GCAGCTCCTG GGGCCTGGAG TCAGGTGGCG GTGCAGAAGG TCAACGCCGC AGGCACAGAT 
CCCAGCTCTC CTGTTGGCTT CGTGCTTGGG GTAGATCTTC TTCACATATT CCCCCTGGAA 
GGAGCAACTT TTCTGTGCCC TGCTGACGTG ACTGACCCGA GAACCTCACA GAGAATCCTC 
GAGGTGCTTC CTGGCAGGAG AGCAGATGTG ATTCTGAGCG ACATGGCGCC CAATGCCACA 
GGGTTCCGGG ACCTCGATCA TGACAGGCTC ATCAGCCTGT GCCTGACCCT TCTCAGCGTG 
ACCCCAGACA TCCTGCAACC TGGGGGGACA TTCCTTTGTA AAACCTGGGC TGGAAGTCAA 
AGCCGTCGGT TACAGAGGAG ACTGACAGAG GAATTCCAGA ATGTAAGGAT CATCAAACCT 
GAAGCCAGCA GGAAAGAGTC ATCAGAAGTG TACTTCTTGG CCACACAGTA CCACGGAAGG 
AAGGGCACTG TGAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRM2 ( NM_013393.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037525.1 Homo sapiens mitochondrial rRNA methyltransferase 2 (MRM2), mRNA.

Price & Availability↑

CloneID	OHu17135
Clone ID Related Accession (Same CDS sequence)	NM_013393.1 , NM_013393.2 , NM_013393.3
Accession Version	NM_013393.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Homo sapiens(human)
Product	rRNA methyltransferase 2, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004971.3, AF093415.1 and AC005282.4. On Aug 31, 2019 this sequence version replaced NM_013393.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794658.1, AF093415.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGT ACTTGAAGCT GGTGTGTGTT TCCTTTCAGC GTCAAGGGTT CCACACTGTT 
GGGAGTCGCT GCAAGAATCG GACAGGCGCT GAGCACCTGT GGCTGACCCG ACATCTCAGG 
GACCCATTTG TGAAGGCTGC GAAGGTGGAG AGTTACCGGT GTCGAAGCGC CTTCAAGCTC 
CTGGAGGTGA ACGAGAGGCA CCAGATTCTG CGGCCCGGCC TTCGGGTGTT AGACTGTGGG 
GCAGCTCCTG GGGCCTGGAG TCAGGTGGCG GTGCAGAAGG TCAACGCCGC AGGCACAGAT 
CCCAGCTCTC CTGTTGGCTT CGTGCTTGGG GTAGATCTTC TTCACATATT CCCCCTGGAA 
GGAGCAACTT TTCTGTGCCC TGCTGACGTG ACTGACCCGA GAACCTCACA GAGAATCCTC 
GAGGTGCTTC CTGGCAGGAG AGCAGATGTG ATTCTGAGCG ACATGGCGCC CAATGCCACA 
GGGTTCCGGG ACCTCGATCA TGACAGGCTC ATCAGCCTGT GCCTGACCCT TCTCAGCGTG 
ACCCCAGACA TCCTGCAACC TGGGGGGACA TTCCTTTGTA AAACCTGGGC TGGAAGTCAA 
AGCCGTCGGT TACAGAGGAG ACTGACAGAG GAATTCCAGA ATGTAAGGAT CATCAAACCT 
GAAGCCAGCA GGAAAGAGTC ATCAGAAGTG TACTTCTTGG CCACACAGTA CCACGGAAGG 
AAGGGCACTG TGAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037525.1
CDS	37..777
Translation	MAGYLKLVCVSFQRQGFHTVGSRCKNRTGAEHLWLTRHLRDPFVKAAKVESYRCRSAFKLLEVNERHQILRPGLRVLDCGAAPGAWSQVAVQKVNAAGTDPSSPVGFVLGVDLLHIFPLEGATFLCPADVTDPRTSQRILEVLPGRRADVILSDMAPNATGFRDLDHDRLISLCLTLLSVTPDILQPGGTFLCKTWAGSQSRRLQRRLTEEFQNVRIIKPEASRKESSEVYFLATQYHGRKGTVKQ

Target ORF information:

RefSeq Version	NM_013393.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial rRNA methyltransferase 2 (MRM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013393.3

ORF Insert Sequence:

ATGGCGGGGT ACTTGAAGCT GGTGTGTGTT TCCTTTCAGC GTCAAGGGTT CCACACTGTT 
GGGAGTCGCT GCAAGAATCG GACAGGCGCT GAGCACCTGT GGCTGACCCG ACATCTCAGG 
GACCCATTTG TGAAGGCTGC GAAGGTGGAG AGTTACCGGT GTCGAAGCGC CTTCAAGCTC 
CTGGAGGTGA ACGAGAGGCA CCAGATTCTG CGGCCCGGCC TTCGGGTGTT AGACTGTGGG 
GCAGCTCCTG GGGCCTGGAG TCAGGTGGCG GTGCAGAAGG TCAACGCCGC AGGCACAGAT 
CCCAGCTCTC CTGTTGGCTT CGTGCTTGGG GTAGATCTTC TTCACATATT CCCCCTGGAA 
GGAGCAACTT TTCTGTGCCC TGCTGACGTG ACTGACCCGA GAACCTCACA GAGAATCCTC 
GAGGTGCTTC CTGGCAGGAG AGCAGATGTG ATTCTGAGCG ACATGGCGCC CAATGCCACA 
GGGTTCCGGG ACCTCGATCA TGACAGGCTC ATCAGCCTGT GCCTGACCCT TCTCAGCGTG 
ACCCCAGACA TCCTGCAACC TGGGGGGACA TTCCTTTGTA AAACCTGGGC TGGAAGTCAA 
AGCCGTCGGT TACAGAGGAG ACTGACAGAG GAATTCCAGA ATGTAAGGAT CATCAAACCT 
GAAGCCAGCA GGAAAGAGTC ATCAGAAGTG TACTTCTTGG CCACACAGTA CCACGGAAGG 
AAGGGCACTG TGAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defective mitochondrial rRNA methyltransferase MRM2 causes MELAS-like clinical syndrome.
Human molecular genetics26(21)4257-4266(2017 Nov)
Garone C,D'Souza AR,Dallabona C,Lodi T,Rebelo-Guiomar P,Rorbach J,Donati MA,Procopio E,Montomoli M,Guerrini R,Zeviani M,Calvo SE,Mootha VK,DiMauro S,Ferrero I,Minczuk M

MiR-542-3p exerts tumor suppressive functions in non-small cell lung cancer cells by upregulating FTSJ2.
Life sciences18887-95(2017 Nov)
Liu B,Li J,Zheng M,Ge J,Li J,Yu P

MRM2 and MRM3 are involved in biogenesis of the large subunit of the mitochondrial ribosome.
Molecular biology of the cell25(17)2542-55(2014 Sep)
Rorbach J,Boesch P,Gammage PA,Nicholls TJ,Pearce SF,Patel D,Hauser A,Perocchi F,Minczuk M

Assignment of 2'-O-methyltransferases to modification sites on the mammalian mitochondrial large subunit 16 S ribosomal RNA (rRNA).
The Journal of biological chemistry289(36)24936-42(2014 Sep)
Lee KW,Bogenhagen DF

FTSJ2, a heat shock-inducible mitochondrial protein, suppresses cell invasion and migration.
PloS one9(3)e90818(2014)
Lai CW,Chen HL,Lin KY,Liu FC,Chong KY,Cheng WT,Chen CM