MRPL18 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPL18
Entrez Gene ID	29074
Full Name	mitochondrial ribosomal protein L18
Synonyms	HSPC071,L18mt,MRP-L18
General protein information	Preferred Names mitochondrial ribosomal protein L18 Names 39S ribosomal protein L18, mitochondrial mitochondrial large ribosomal subunit protein uL18m
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q25.3
Summary	This nuclear gene encodes a protein component of the larger 39S subunit of mitochondrial ribosome. This protein may also aid in the import of nuclear-encoded 5S rRNA into mitochondria. Alternative splicing results in multiple transcript variants, most of which are not predicted to encode a protein. A pseudogene of this gene is found on chromosome 16. [provided by RefSeq, Jan 2016].
Disorder MIM:	611831

mRNA and Protein(s)

mRNA	Protein	Name
NM_014161.5	NP_054880.2	39S ribosomal protein L18, mitochondrial isoform 1
NM_014161.4	NP_054880.2	39S ribosomal protein L18, mitochondrial isoform 1
NM_014161.3	NP_054880.2	39S ribosomal protein L18, mitochondrial
NM_001318817.1	NP_001305746.1	39S ribosomal protein L18, mitochondrial isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MRPL18	XP_533465.2
Rattus norvegicus (Norway rat)	Mrpl18	NP_001099675.1
Xenopus tropicalis (tropical clawed frog)	mrpl18	NP_989288.1
Pan troglodytes (chimpanzee)	MRPL18	XP_001150906.1
Gallus gallus (chicken)	MRPL18	XP_419623.1
Drosophila melanogaster (fruit fly)	mRpL18	NP_610818.1
	LOC697510	XP_001086086.2
Mus musculus (house mouse)	Mrpl18	NP_080586.1
Homo sapiens (human)	MRPL18	NP_054880.2
	MRPL18	XP_001098325.1
Danio rerio (zebrafish)	mrpl18	NP_001070798.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004709	XP_554644.2
Bos taurus (cattle)	MRPL18	NP_001029780.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Brown A, Rathore S, Kimanius D, Aibara S, Bai XC, Rorbach J, Amunts A, Ramakrishnan V
Nature structural & molecular biology24(10)866-869(2017 Oct)

Structure of the large ribosomal subunit from human mitochondria.
Brown A, Amunts A, Bai XC, Sugimoto Y, Edwards PC, Murshudov G, Scheres SHW, Ramakrishnan V
Science (New York, N.Y.)346(6210)718-722(2014 Nov)

Biological significance of 5S rRNA import into human mitochondria: role of ribosomal protein MRP-L18.
Smirnov A, Entelis N, Martin RP, Tarassov I
Genes & development25(12)1289-305(2011 Jun)

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Zhang Z, Gerstein M
Genomics81(5)468-80(2003 May)

Expressed sequence tag analysis of adult human lens for the NEIBank Project: over 2000 non-redundant transcripts, novel genes and splice variants.
Wistow G, Bernstein SL, Wyatt MK, Behal A, Touchman JW, Bouffard G, Smith D, Peterson K
Molecular vision8171-84(2002 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following MRPL18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPL18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31459	NM_014161.5 Latest version!	Homo sapiens mitochondrial ribosomal protein L18 (MRPL18), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu40792	NM_001318817.1 Latest version!	Homo sapiens mitochondrial ribosomal protein L18 (MRPL18), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu31459	NM_014161.4	Homo sapiens mitochondrial ribosomal protein L18 (MRPL18), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu31459	NM_014161.3	Homo sapiens mitochondrial ribosomal protein L18 (MRPL18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPL18 ( NM_014161.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_054880.2 Homo sapiens mitochondrial ribosomal protein L18 (MRPL18), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31459
Clone ID Related Accession (Same CDS sequence)	NM_014161.5 , NM_014161.4 , NM_014161.3
Accession Version	NM_014161.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	39S ribosomal protein L18, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU932963.1, AF161556.1 and BC001623.1. On Nov 22, 2018 this sequence version replaced NM_014161.4. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1163658.506051.1, SRR5189661.14037.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000367034.5/ ENSP00000356001.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGCTTC GGTCGCGGTT TTGGGGGTTG TTCTCGGTTT GCAGGAACCC TGGGTGCAGG 
TTCGCAGCCC TGTCAACCAG CTCCGAGCCG GCAGCGAAAC CTGAAGTGGA CCCTGTGGAA 
AATGAAGCTG TCGCCCCAGA ATTCACCAAC CGGAACCCCC GGAACCTGGA GCTTTTATCT 
GTAGCCAGGA AAGAGCGGGG CTGGCGGACG GTGTTTCCCT CCCGTGAGTT CTGGCACAGG 
TTGCGAGTTA TAAGGACTCA GCATCATGTA GAAGCACTTG TGGAGCATCA GAATGGCAAG 
GTTGTGGTTT CGGCCTCCAC TCGTGAGTGG GCTATTAAAA AGCACCTTTA TAGTACCAGA 
AATGTGGTGG CTTGTGAGAG TATAGGACGA GTGCTGGCAC AGAGATGCTT AGAGGCGGGA 
ATCAACTTCA TGGTCTACCA ACCAACCCCG TGGGAGGCAG CCTCAGACTC GATGAAACGA 
CTACAAAGTG CCATGACAGA AGGTGGTGTG GTTCTACGGG AACCTCAGAG AATCTATGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054880.2
CDS	122..664
Translation	MALRSRFWGLFSVCRNPGCRFAALSTSSEPAAKPEVDPVENEAVAPEFTNRNPRNLELLSVARKERGWRTVFPSREFWHRLRVIRTQHHVEALVEHQNGKVVVSASTREWAIKKHLYSTRNVVACESIGRVLAQRCLEAGINFMVYQPTPWEAASDSMKRLQSAMTEGGVVLREPQRIYE

Target ORF information:

RefSeq Version	NM_014161.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L18 (MRPL18), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014161.5

ORF Insert Sequence:

ATGGCGCTTC GGTCGCGGTT TTGGGGGTTG TTCTCGGTTT GCAGGAACCC TGGGTGCAGG 
TTCGCAGCCC TGTCAACCAG CTCCGAGCCG GCAGCGAAAC CTGAAGTGGA CCCTGTGGAA 
AATGAAGCTG TCGCCCCAGA ATTCACCAAC CGGAACCCCC GGAACCTGGA GCTTTTATCT 
GTAGCCAGGA AAGAGCGGGG CTGGCGGACG GTGTTTCCCT CCCGTGAGTT CTGGCACAGG 
TTGCGAGTTA TAAGGACTCA GCATCATGTA GAAGCACTTG TGGAGCATCA GAATGGCAAG 
GTTGTGGTTT CGGCCTCCAC TCGTGAGTGG GCTATTAAAA AGCACCTTTA TAGTACCAGA 
AATGTGGTGG CTTGTGAGAG TATAGGACGA GTGCTGGCAC AGAGATGCTT AGAGGCGGGA 
ATCAACTTCA TGGTCTACCA ACCAACCCCG TGGGAGGCAG CCTCAGACTC GATGAAACGA 
CTACAAAGTG CCATGACAGA AGGTGGTGTG GTTCTACGGG AACCTCAGAG AATCTATGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL18 ( NM_014161.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_054880.2 Homo sapiens mitochondrial ribosomal protein L18 (MRPL18), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31459
Clone ID Related Accession (Same CDS sequence)	NM_014161.5 , NM_014161.4 , NM_014161.3
Accession Version	NM_014161.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	39S ribosomal protein L18, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL135914.25, BU932963.1, AF161556.1 and BC001623.1. On Jan 14, 2016 this sequence version replaced NM_014161.3. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1163658.506051.1, ERR279863.5800.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTTC GGTCGCGGTT TTGGGGGTTG TTCTCGGTTT GCAGGAACCC TGGGTGCAGG 
TTCGCAGCCC TGTCAACCAG CTCCGAGCCG GCAGCGAAAC CTGAAGTGGA CCCTGTGGAA 
AATGAAGCTG TCGCCCCAGA ATTCACCAAC CGGAACCCCC GGAACCTGGA GCTTTTATCT 
GTAGCCAGGA AAGAGCGGGG CTGGCGGACG GTGTTTCCCT CCCGTGAGTT CTGGCACAGG 
TTGCGAGTTA TAAGGACTCA GCATCATGTA GAAGCACTTG TGGAGCATCA GAATGGCAAG 
GTTGTGGTTT CGGCCTCCAC TCGTGAGTGG GCTATTAAAA AGCACCTTTA TAGTACCAGA 
AATGTGGTGG CTTGTGAGAG TATAGGACGA GTGCTGGCAC AGAGATGCTT AGAGGCGGGA 
ATCAACTTCA TGGTCTACCA ACCAACCCCG TGGGAGGCAG CCTCAGACTC GATGAAACGA 
CTACAAAGTG CCATGACAGA AGGTGGTGTG GTTCTACGGG AACCTCAGAG AATCTATGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054880.2
CDS	143..685
Translation	MALRSRFWGLFSVCRNPGCRFAALSTSSEPAAKPEVDPVENEAVAPEFTNRNPRNLELLSVARKERGWRTVFPSREFWHRLRVIRTQHHVEALVEHQNGKVVVSASTREWAIKKHLYSTRNVVACESIGRVLAQRCLEAGINFMVYQPTPWEAASDSMKRLQSAMTEGGVVLREPQRIYE

Target ORF information:

RefSeq Version	NM_014161.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L18 (MRPL18), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014161.4

ORF Insert Sequence:

ATGGCGCTTC GGTCGCGGTT TTGGGGGTTG TTCTCGGTTT GCAGGAACCC TGGGTGCAGG 
TTCGCAGCCC TGTCAACCAG CTCCGAGCCG GCAGCGAAAC CTGAAGTGGA CCCTGTGGAA 
AATGAAGCTG TCGCCCCAGA ATTCACCAAC CGGAACCCCC GGAACCTGGA GCTTTTATCT 
GTAGCCAGGA AAGAGCGGGG CTGGCGGACG GTGTTTCCCT CCCGTGAGTT CTGGCACAGG 
TTGCGAGTTA TAAGGACTCA GCATCATGTA GAAGCACTTG TGGAGCATCA GAATGGCAAG 
GTTGTGGTTT CGGCCTCCAC TCGTGAGTGG GCTATTAAAA AGCACCTTTA TAGTACCAGA 
AATGTGGTGG CTTGTGAGAG TATAGGACGA GTGCTGGCAC AGAGATGCTT AGAGGCGGGA 
ATCAACTTCA TGGTCTACCA ACCAACCCCG TGGGAGGCAG CCTCAGACTC GATGAAACGA 
CTACAAAGTG CCATGACAGA AGGTGGTGTG GTTCTACGGG AACCTCAGAG AATCTATGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL18 ( NM_014161.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_054880.2 Homo sapiens mitochondrial ribosomal protein L18 (MRPL18), mRNA.

Price & Availability↑

CloneID	OHu31459
Clone ID Related Accession (Same CDS sequence)	NM_014161.5 , NM_014161.4 , NM_014161.3
Accession Version	NM_014161.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	39S ribosomal protein L18, mitochondrial
Comment

ATGGCGCTTC GGTCGCGGTT TTGGGGGTTG TTCTCGGTTT GCAGGAACCC TGGGTGCAGG 
TTCGCAGCCC TGTCAACCAG CTCCGAGCCG GCAGCGAAAC CTGAAGTGGA CCCTGTGGAA 
AATGAAGCTG TCGCCCCAGA ATTCACCAAC CGGAACCCCC GGAACCTGGA GCTTTTATCT 
GTAGCCAGGA AAGAGCGGGG CTGGCGGACG GTGTTTCCCT CCCGTGAGTT CTGGCACAGG 
TTGCGAGTTA TAAGGACTCA GCATCATGTA GAAGCACTTG TGGAGCATCA GAATGGCAAG 
GTTGTGGTTT CGGCCTCCAC TCGTGAGTGG GCTATTAAAA AGCACCTTTA TAGTACCAGA 
AATGTGGTGG CTTGTGAGAG TATAGGACGA GTGCTGGCAC AGAGATGCTT AGAGGCGGGA 
ATCAACTTCA TGGTCTACCA ACCAACCCCG TGGGAGGCAG CCTCAGACTC GATGAAACGA 
CTACAAAGTG CCATGACAGA AGGTGGTGTG GTTCTACGGG AACCTCAGAG AATCTATGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054880.2
Translation	MALRSRFWGLFSVCRNPGCRFAALSTSSEPAAKPEVDPVENEAVAPEFTNRNPRNLELLSVARKERGWRTVFPSREFWHRLRVIRTQHHVEALVEHQNGKVVVSASTREWAIKKHLYSTRNVVACESIGRVLAQRCLEAGINFMVYQPTPWEAASDSMKRLQSAMTEGGVVLREPQRIYE

Target ORF information:

RefSeq Version	NM_014161.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens mitochondrial ribosomal protein L18 (MRPL18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014161.3

ORF Insert Sequence:

ATGGCGCTTC GGTCGCGGTT TTGGGGGTTG TTCTCGGTTT GCAGGAACCC TGGGTGCAGG 
TTCGCAGCCC TGTCAACCAG CTCCGAGCCG GCAGCGAAAC CTGAAGTGGA CCCTGTGGAA 
AATGAAGCTG TCGCCCCAGA ATTCACCAAC CGGAACCCCC GGAACCTGGA GCTTTTATCT 
GTAGCCAGGA AAGAGCGGGG CTGGCGGACG GTGTTTCCCT CCCGTGAGTT CTGGCACAGG 
TTGCGAGTTA TAAGGACTCA GCATCATGTA GAAGCACTTG TGGAGCATCA GAATGGCAAG 
GTTGTGGTTT CGGCCTCCAC TCGTGAGTGG GCTATTAAAA AGCACCTTTA TAGTACCAGA 
AATGTGGTGG CTTGTGAGAG TATAGGACGA GTGCTGGCAC AGAGATGCTT AGAGGCGGGA 
ATCAACTTCA TGGTCTACCA ACCAACCCCG TGGGAGGCAG CCTCAGACTC GATGAAACGA 
CTACAAAGTG CCATGACAGA AGGTGGTGTG GTTCTACGGG AACCTCAGAG AATCTATGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPL18 ( NM_001318817.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305746.1 Homo sapiens mitochondrial ribosomal protein L18 (MRPL18), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu40792
Clone ID Related Accession (Same CDS sequence)	NM_001318817.1
Accession Version	NM_001318817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	39S ribosomal protein L18, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL135914.25, BG548185.1 and BC001623.1. On Jan 14, 2016 this sequence version replaced XM_006715466.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a shorter, distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.181307.1, SRR1163658.84780.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTTC GGTCGCGGTT TTGGGGGTTG TTCTCGGTTT GCAGGAACCC TGGGTGCAGG 
TTCGCAGCCC TGTCAACCAG CTCCGAGCCG GCAGCGAAAC CTGAAGTGGA CCCTGTGGAA 
AATGAAGCTG TCGCCCCAGA ATTCACCAAC CGGAACCCCC GGAACCTGGA GCTTTTATCT 
GTAGCCAGGA AAGAGCGGGG CTGGCGGACG GTGTTTCCCT CCCGTGAGTT CTGGCACAGG 
TTGCGAGTTA TAAGGACTCA GCATCATGTA GAAGCACTTG TGGAGCATCA GAATGGCAAG 
GTTGTGGTTT CGGCCTCCAC TCATGAAACG ACTACAAAGT GCCATGACAG AAGGTGGTGT 
GGTTCTACGG GAACCTCAGA GAATCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305746.1
CDS	143..532
Translation	MALRSRFWGLFSVCRNPGCRFAALSTSSEPAAKPEVDPVENEAVAPEFTNRNPRNLELLSVARKERGWRTVFPSREFWHRLRVIRTQHHVEALVEHQNGKVVVSASTHETTTKCHDRRWCGSTGTSENL

Target ORF information:

RefSeq Version	NM_001318817.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein L18 (MRPL18), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318817.1

ORF Insert Sequence:

ATGGCGCTTC GGTCGCGGTT TTGGGGGTTG TTCTCGGTTT GCAGGAACCC TGGGTGCAGG 
TTCGCAGCCC TGTCAACCAG CTCCGAGCCG GCAGCGAAAC CTGAAGTGGA CCCTGTGGAA 
AATGAAGCTG TCGCCCCAGA ATTCACCAAC CGGAACCCCC GGAACCTGGA GCTTTTATCT 
GTAGCCAGGA AAGAGCGGGG CTGGCGGACG GTGTTTCCCT CCCGTGAGTT CTGGCACAGG 
TTGCGAGTTA TAAGGACTCA GCATCATGTA GAAGCACTTG TGGAGCATCA GAATGGCAAG 
GTTGTGGTTT CGGCCTCCAC TCATGAAACG ACTACAAAGT GCCATGACAG AAGGTGGTGT 
GGTTCTACGG GAACCTCAGA GAATCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures of the human mitochondrial ribosome in native states of assembly.
Nature structural & molecular biology24(10)866-869(2017 Oct)
Brown A,Rathore S,Kimanius D,Aibara S,Bai XC,Rorbach J,Amunts A,Ramakrishnan V

Structure of the large ribosomal subunit from human mitochondria.
Science (New York, N.Y.)346(6210)718-722(2014 Nov)
Brown A,Amunts A,Bai XC,Sugimoto Y,Edwards PC,Murshudov G,Scheres SHW,Ramakrishnan V

Biological significance of 5S rRNA import into human mitochondria: role of ribosomal protein MRP-L18.
Genes & development25(12)1289-305(2011 Jun)
Smirnov A,Entelis N,Martin RP,Tarassov I

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Genomics81(5)468-80(2003 May)
Zhang Z,Gerstein M

Expressed sequence tag analysis of adult human lens for the NEIBank Project: over 2000 non-redundant transcripts, novel genes and splice variants.
Molecular vision8171-84(2002 Jun)
Wistow G,Bernstein SL,Wyatt MK,Behal A,Touchman JW,Bouffard G,Smith D,Peterson K