FFAR3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FFAR3
Entrez Gene ID	2865
Full Name	free fatty acid receptor 3
Synonyms	FFA3R,GPR41,GPR42
General protein information	Preferred Names free fatty acid receptor 3 Names free fatty acid receptor 3 G-protein coupled receptor 41
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.12
Disorder MIM:	603821

mRNA and Protein(s)

mRNA	Protein	Name
NM_005304.4	NP_005295.1	free fatty acid receptor 3
NM_005304.3	NP_005295.1	free fatty acid receptor 3
XM_011526858.2	XP_011525160.1	free fatty acid receptor 3 isoform X1
NM_005304.5	NP_005295.1	free fatty acid receptor 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	FFAR3	XP_005616768.1
Pan troglodytes (chimpanzee)	FFAR3	XP_001159667.1
Bos taurus (cattle)	FFAR3	NP_001138705.1
Homo sapiens (human)	FFAR3	NP_005295.1
	FFAR3	XP_001094758.1
Mus musculus (house mouse)	Ffar3	NP_001028488.1
Rattus norvegicus (Norway rat)	Ffar3	NP_001102382.1
Danio rerio (zebrafish)	LOC100333904	XP_002665089.1
Xenopus tropicalis (tropical clawed frog)	LOC100489919	XP_002938262.1
Xenopus tropicalis (tropical clawed frog)	LOC100489751	XP_002938261.2
Xenopus tropicalis (tropical clawed frog)	LOC101735161	XP_004920890.1

Gene Ontology
Bibliography

Related articles in PubMed

FFAR2-FFAR3 receptor heteromerization modulates short-chain fatty acid sensing.
Ang Z, Xiong D, Wu M, Ding JL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology32(1)289-303(2018 Jan)

IL-1? and TNF? inhibit GPR120 (FFAR4) and stimulate GPR84 (EX33) and GPR41 (FFAR3) fatty acid receptor expression in human adipocytes: implications for the anti-inflammatory action of n-3 fatty acids.
Muredda L, K?pczy?ska MA, Zaibi MS, Alomar SY, Trayhurn P
Archives of physiology and biochemistry1-12(2017 Aug)

Short-chain fatty acids, GPR41 and GPR43 ligands, inhibit TNF-?-induced MCP-1 expression by modulating p38 and JNK signaling pathways in human renal cortical epithelial cells.
Kobayashi M, Mikami D, Kimura H, Kamiyama K, Morikawa Y, Yokoi S, Kasuno K, Takahashi N, Taniguchi T, Iwano M
Biochemical and biophysical research communications486(2)499-505(2017 04)

Soluble Fibre Meal Challenge Reduces Airway Inflammation and Expression of GPR43 and GPR41 in Asthma.
Halnes I, Baines KJ, Berthon BS, MacDonald-Wicks LK, Gibson PG, Wood LG
Nutrients9(1)(2017 Jan)

Human GPR42 is a transcribed multisite variant that exhibits copy number polymorphism and is functional when heterologously expressed.
Puhl HL 3rd, Won YJ, Lu VB, Ikeda SR
Scientific reports512880(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Short-chain fatty acids lowered TNF-alpha-induced MCP-1 expression by reducing phosphorylation of p38 MAPK and JNK in a GPR41/GRP43-dependent manner in renal cortical epithelial cells.
Title: Short-chain fatty acids, GPR41 and GPR43 ligands, inhibit TNF-?-induced MCP-1 expression by modulating p38 and JNK signaling pathways in human renal cortical epithelial cells.

a single dose of soluble fibre was able to significantly reduce airway inflammation in stable asthma by downregulating GPR43 and GPR41
Title: Soluble Fibre Meal Challenge Reduces Airway Inflammation and Expression of GPR43 and GPR41 in Asthma.

Our data suggest that GPR42 be reclassified as a functioning gene and that recognition of sequence and copy number polymorphism of the FFAR3/GPR42 complex be considered during genetic and pharmacological investigation of these receptors.
Title: Human GPR42 is a transcribed multisite variant that exhibits copy number polymorphism and is functional when heterologously expressed.

FFAR3 is expressed in pancreatic beta cells and mediates an inhibition of insulin secretion by coupling to Gi-type G proteins.
Title: Loss of FFA2 and FFA3 increases insulin secretion and improves glucose tolerance in type 2 diabetes.

a significant correlation between a higher body mass index and lower methylation in the promoter region of FFAR3 in type 2 diabetes and obesity patients
Title: Effects of short chain fatty acid producing bacteria on epigenetic regulation of FFAR3 in type 2 diabetes and obesity.

The following FFAR3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FFAR3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24442	NM_005304.5 Latest version!	Homo sapiens free fatty acid receptor 3 (FFAR3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu58029	XM_011526858.2 Latest version!	Homo sapiens free fatty acid receptor 3 (FFAR3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu24442	NM_005304.3	Homo sapiens free fatty acid receptor 3 (FFAR3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu24442	NM_005304.4	Homo sapiens free fatty acid receptor 3 (FFAR3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FFAR3 ( NM_005304.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005295.1 Homo sapiens free fatty acid receptor 3 (FFAR3), mRNA.

Price & Availability↑

CloneID	OHu24442
Clone ID Related Accession (Same CDS sequence)	NM_005304.4 , NM_005304.3 , NM_005304.5
Accession Version	NM_005304.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	free fatty acid receptor 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR004234.1, BC035657.1 and AC002511.1. On Jan 18, 2017 this sequence version replaced NM_005304.3. Sequence Note:. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035657.1, AK290710.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATACAG GCCCCGACCA GTCCTACTTC TCCGGCAATC ACTGGTTCGT CTTCTCGGTG 
TACCTTCTCA CTTTCCTGGT GGGGCTCCCC CTCAACCTGC TGGCCCTGGT GGTCTTCGTG 
GGCAAGCTGC AGCGCCGCCC GGTGGCCGTG GACGTGCTCC TGCTCAACCT GACCGCCTCG 
GACCTGCTCC TGCTGCTGTT CCTGCCTTTC CGCATGGTGG AGGCAGCCAA TGGCATGCAC 
TGGCCCCTGC CCTTCATCCT CTGCCCACTC TCTGGATTCA TCTTCTTCAC CACCATCTAT 
CTCACCGCCC TCTTCCTGGC AGCTGTGAGC ATTGAACGCT TCCTGAGTGT GGCCCACCCA 
CTGTGGTACA AGACCCGGCC GAGGCTGGGG CAGGCAGGTC TGGTGAGTGT GGCCTGCTGG 
CTGTTGGCCT CTGCTCACTG CAGCGTGGTC TACGTCATAG AATTCTCAGG GGACATCTCC 
CACAGCCAGG GCACCAATGG GACCTGCTAC CTGGAGTTCC GGAAGGACCA GCTAGCCATC 
CTCCTGCCCG TGCGGCTGGA GATGGCTGTG GTCCTCTTTG TGGTCCCGCT GATCATCACC 
AGCTACTGCT ACAGCCGCCT GGTGTGGATC CTCGGCAGAG GGGGCAGCCA CCGCCGGCAG 
AGGAGGGTGG CGGGGCTGTT GGCGGCCACG CTGCTCAACT TCCTTGTCTG CTTTGGGCCC 
TACAACGTGT CCCATGTCGT GGGCTATATC TGCGGTGAAA GCCCGGCGTG GAGGATCTAC 
GTGACGCTTC TCAGCACCCT GAACTCCTGT GTCGACCCCT TTGTCTACTA CTTCTCCTCC 
TCCGGGTTCC AAGCCGACTT TCATGAGCTG CTGAGGAGGT TGTGTGGGCT CTGGGGCCAG 
TGGCAGCAGG AGAGCAGCAT GGAGCTGAAG GAGCAGAAGG GAGGGGAGGA GCAGAGAGCG 
GACCGACCAG CTGAAAGAAA GACCAGTGAA CACTCACAGG GCTGTGGAAC TGGTGGCCAG 
GTGGCCTGTG CTGAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005295.1
CDS	202..1242
Translation	MDTGPDQSYFSGNHWFVFSVYLLTFLVGLPLNLLALVVFVGKLQRRPVAVDVLLLNLTASDLLLLLFLPFRMVEAANGMHWPLPFILCPLSGFIFFTTIYLTALFLAAVSIERFLSVAHPLWYKTRPRLGQAGLVSVACWLLASAHCSVVYVIEFSGDISHSQGTNGTCYLEFRKDQLAILLPVRLEMAVVLFVVPLIITSYCYSRLVWILGRGGSHRRQRRVAGLLAATLLNFLVCFGPYNVSHVVGYICGESPAWRIYVTLLSTLNSCVDPFVYYFSSSGFQADFHELLRRLCGLWGQWQQESSMELKEQKGGEEQRADRPAERKTSEHSQGCGTGGQVACAES

Target ORF information:

RefSeq Version	NM_005304.4
Organism	Homo sapiens(human)
Definition	Homo sapiens free fatty acid receptor 3 (FFAR3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005304.4

ORF Insert Sequence:

ATGGATACAG GCCCCGACCA GTCCTACTTC TCCGGCAATC ACTGGTTCGT CTTCTCGGTG 
TACCTTCTCA CTTTCCTGGT GGGGCTCCCC CTCAACCTGC TGGCCCTGGT GGTCTTCGTG 
GGCAAGCTGC AGCGCCGCCC GGTGGCCGTG GACGTGCTCC TGCTCAACCT GACCGCCTCG 
GACCTGCTCC TGCTGCTGTT CCTGCCTTTC CGCATGGTGG AGGCAGCCAA TGGCATGCAC 
TGGCCCCTGC CCTTCATCCT CTGCCCACTC TCTGGATTCA TCTTCTTCAC CACCATCTAT 
CTCACCGCCC TCTTCCTGGC AGCTGTGAGC ATTGAACGCT TCCTGAGTGT GGCCCACCCA 
CTGTGGTACA AGACCCGGCC GAGGCTGGGG CAGGCAGGTC TGGTGAGTGT GGCCTGCTGG 
CTGTTGGCCT CTGCTCACTG CAGCGTGGTC TACGTCATAG AATTCTCAGG GGACATCTCC 
CACAGCCAGG GCACCAATGG GACCTGCTAC CTGGAGTTCC GGAAGGACCA GCTAGCCATC 
CTCCTGCCCG TGCGGCTGGA GATGGCTGTG GTCCTCTTTG TGGTCCCGCT GATCATCACC 
AGCTACTGCT ACAGCCGCCT GGTGTGGATC CTCGGCAGAG GGGGCAGCCA CCGCCGGCAG 
AGGAGGGTGG CGGGGCTGTT GGCGGCCACG CTGCTCAACT TCCTTGTCTG CTTTGGGCCC 
TACAACGTGT CCCATGTCGT GGGCTATATC TGCGGTGAAA GCCCGGCGTG GAGGATCTAC 
GTGACGCTTC TCAGCACCCT GAACTCCTGT GTCGACCCCT TTGTCTACTA CTTCTCCTCC 
TCCGGGTTCC AAGCCGACTT TCATGAGCTG CTGAGGAGGT TGTGTGGGCT CTGGGGCCAG 
TGGCAGCAGG AGAGCAGCAT GGAGCTGAAG GAGCAGAAGG GAGGGGAGGA GCAGAGAGCG 
GACCGACCAG CTGAAAGAAA GACCAGTGAA CACTCACAGG GCTGTGGAAC TGGTGGCCAG 
GTGGCCTGTG CTGAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FFAR3 ( NM_005304.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005295.1 Homo sapiens free fatty acid receptor 3 (FFAR3), mRNA.

Price & Availability↑

CloneID	OHu24442
Clone ID Related Accession (Same CDS sequence)	NM_005304.4 , NM_005304.3 , NM_005304.5
Accession Version	NM_005304.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-03
Organism	Homo sapiens(Human)
Product	free fatty acid receptor 3
Comment

ATGGATACAG GCCCCGACCA GTCCTACTTC TCCGGCAATC ACTGGTTCGT CTTCTCGGTG 
TACCTTCTCA CTTTCCTGGT GGGGCTCCCC CTCAACCTGC TGGCCCTGGT GGTCTTCGTG 
GGCAAGCTGC AGCGCCGCCC GGTGGCCGTG GACGTGCTCC TGCTCAACCT GACCGCCTCG 
GACCTGCTCC TGCTGCTGTT CCTGCCTTTC CGCATGGTGG AGGCAGCCAA TGGCATGCAC 
TGGCCCCTGC CCTTCATCCT CTGCCCACTC TCTGGATTCA TCTTCTTCAC CACCATCTAT 
CTCACCGCCC TCTTCCTGGC AGCTGTGAGC ATTGAACGCT TCCTGAGTGT GGCCCACCCA 
CTGTGGTACA AGACCCGGCC GAGGCTGGGG CAGGCAGGTC TGGTGAGTGT GGCCTGCTGG 
CTGTTGGCCT CTGCTCACTG CAGCGTGGTC TACGTCATAG AATTCTCAGG GGACATCTCC 
CACAGCCAGG GCACCAATGG GACCTGCTAC CTGGAGTTCC GGAAGGACCA GCTAGCCATC 
CTCCTGCCCG TGCGGCTGGA GATGGCTGTG GTCCTCTTTG TGGTCCCGCT GATCATCACC 
AGCTACTGCT ACAGCCGCCT GGTGTGGATC CTCGGCAGAG GGGGCAGCCA CCGCCGGCAG 
AGGAGGGTGG CGGGGCTGTT GGCGGCCACG CTGCTCAACT TCCTTGTCTG CTTTGGGCCC 
TACAACGTGT CCCATGTCGT GGGCTATATC TGCGGTGAAA GCCCGGCGTG GAGGATCTAC 
GTGACGCTTC TCAGCACCCT GAACTCCTGT GTCGACCCCT TTGTCTACTA CTTCTCCTCC 
TCCGGGTTCC AAGCCGACTT TCATGAGCTG CTGAGGAGGT TGTGTGGGCT CTGGGGCCAG 
TGGCAGCAGG AGAGCAGCAT GGAGCTGAAG GAGCAGAAGG GAGGGGAGGA GCAGAGAGCG 
GACCGACCAG CTGAAAGAAA GACCAGTGAA CACTCACAGG GCTGTGGAAC TGGTGGCCAG 
GTGGCCTGTG CTGAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005295.1
Translation	MDTGPDQSYFSGNHWFVFSVYLLTFLVGLPLNLLALVVFVGKLQRRPVAVDVLLLNLTASDLLLLLFLPFRMVEAANGMHWPLPFILCPLSGFIFFTTIYLTALFLAAVSIERFLSVAHPLWYKTRPRLGQAGLVSVACWLLASAHCSVVYVIEFSGDISHSQGTNGTCYLEFRKDQLAILLPVRLEMAVVLFVVPLIITSYCYSRLVWILGRGGSHRRQRRVAGLLAATLLNFLVCFGPYNVSHVVGYICGESPAWRIYVTLLSTLNSCVDPFVYYFSSSGFQADFHELLRRLCGLWGQWQQESSMELKEQKGGEEQRADRPAERKTSEHSQGCGTGGQVACAES

Target ORF information:

RefSeq Version	NM_005304.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens free fatty acid receptor 3 (FFAR3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005304.3

ORF Insert Sequence:

ATGGATACAG GCCCCGACCA GTCCTACTTC TCCGGCAATC ACTGGTTCGT CTTCTCGGTG 
TACCTTCTCA CTTTCCTGGT GGGGCTCCCC CTCAACCTGC TGGCCCTGGT GGTCTTCGTG 
GGCAAGCTGC AGCGCCGCCC GGTGGCCGTG GACGTGCTCC TGCTCAACCT GACCGCCTCG 
GACCTGCTCC TGCTGCTGTT CCTGCCTTTC CGCATGGTGG AGGCAGCCAA TGGCATGCAC 
TGGCCCCTGC CCTTCATCCT CTGCCCACTC TCTGGATTCA TCTTCTTCAC CACCATCTAT 
CTCACCGCCC TCTTCCTGGC AGCTGTGAGC ATTGAACGCT TCCTGAGTGT GGCCCACCCA 
CTGTGGTACA AGACCCGGCC GAGGCTGGGG CAGGCAGGTC TGGTGAGTGT GGCCTGCTGG 
CTGTTGGCCT CTGCTCACTG CAGCGTGGTC TACGTCATAG AATTCTCAGG GGACATCTCC 
CACAGCCAGG GCACCAATGG GACCTGCTAC CTGGAGTTCC GGAAGGACCA GCTAGCCATC 
CTCCTGCCCG TGCGGCTGGA GATGGCTGTG GTCCTCTTTG TGGTCCCGCT GATCATCACC 
AGCTACTGCT ACAGCCGCCT GGTGTGGATC CTCGGCAGAG GGGGCAGCCA CCGCCGGCAG 
AGGAGGGTGG CGGGGCTGTT GGCGGCCACG CTGCTCAACT TCCTTGTCTG CTTTGGGCCC 
TACAACGTGT CCCATGTCGT GGGCTATATC TGCGGTGAAA GCCCGGCGTG GAGGATCTAC 
GTGACGCTTC TCAGCACCCT GAACTCCTGT GTCGACCCCT TTGTCTACTA CTTCTCCTCC 
TCCGGGTTCC AAGCCGACTT TCATGAGCTG CTGAGGAGGT TGTGTGGGCT CTGGGGCCAG 
TGGCAGCAGG AGAGCAGCAT GGAGCTGAAG GAGCAGAAGG GAGGGGAGGA GCAGAGAGCG 
GACCGACCAG CTGAAAGAAA GACCAGTGAA CACTCACAGG GCTGTGGAAC TGGTGGCCAG 
GTGGCCTGTG CTGAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FFAR3 ( NM_005304.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005295.1 Homo sapiens free fatty acid receptor 3 (FFAR3), mRNA.

Price & Availability↑

CloneID	OHu24442
Clone ID Related Accession (Same CDS sequence)	NM_005304.4 , NM_005304.3 , NM_005304.5
Accession Version	NM_005304.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	free fatty acid receptor 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR004234.1, BC035657.1 and AC002511.1. On May 17, 2019 this sequence version replaced NM_005304.4. Sequence Note:. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035657.1, AK290710.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000327809.5/ ENSP00000328230.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATACAG GCCCCGACCA GTCCTACTTC TCCGGCAATC ACTGGTTCGT CTTCTCGGTG 
TACCTTCTCA CTTTCCTGGT GGGGCTCCCC CTCAACCTGC TGGCCCTGGT GGTCTTCGTG 
GGCAAGCTGC AGCGCCGCCC GGTGGCCGTG GACGTGCTCC TGCTCAACCT GACCGCCTCG 
GACCTGCTCC TGCTGCTGTT CCTGCCTTTC CGCATGGTGG AGGCAGCCAA TGGCATGCAC 
TGGCCCCTGC CCTTCATCCT CTGCCCACTC TCTGGATTCA TCTTCTTCAC CACCATCTAT 
CTCACCGCCC TCTTCCTGGC AGCTGTGAGC ATTGAACGCT TCCTGAGTGT GGCCCACCCA 
CTGTGGTACA AGACCCGGCC GAGGCTGGGG CAGGCAGGTC TGGTGAGTGT GGCCTGCTGG 
CTGTTGGCCT CTGCTCACTG CAGCGTGGTC TACGTCATAG AATTCTCAGG GGACATCTCC 
CACAGCCAGG GCACCAATGG GACCTGCTAC CTGGAGTTCC GGAAGGACCA GCTAGCCATC 
CTCCTGCCCG TGCGGCTGGA GATGGCTGTG GTCCTCTTTG TGGTCCCGCT GATCATCACC 
AGCTACTGCT ACAGCCGCCT GGTGTGGATC CTCGGCAGAG GGGGCAGCCA CCGCCGGCAG 
AGGAGGGTGG CGGGGCTGTT GGCGGCCACG CTGCTCAACT TCCTTGTCTG CTTTGGGCCC 
TACAACGTGT CCCATGTCGT GGGCTATATC TGCGGTGAAA GCCCGGCGTG GAGGATCTAC 
GTGACGCTTC TCAGCACCCT GAACTCCTGT GTCGACCCCT TTGTCTACTA CTTCTCCTCC 
TCCGGGTTCC AAGCCGACTT TCATGAGCTG CTGAGGAGGT TGTGTGGGCT CTGGGGCCAG 
TGGCAGCAGG AGAGCAGCAT GGAGCTGAAG GAGCAGAAGG GAGGGGAGGA GCAGAGAGCG 
GACCGACCAG CTGAAAGAAA GACCAGTGAA CACTCACAGG GCTGTGGAAC TGGTGGCCAG 
GTGGCCTGTG CTGAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005295.1
CDS	202..1242
Translation	MDTGPDQSYFSGNHWFVFSVYLLTFLVGLPLNLLALVVFVGKLQRRPVAVDVLLLNLTASDLLLLLFLPFRMVEAANGMHWPLPFILCPLSGFIFFTTIYLTALFLAAVSIERFLSVAHPLWYKTRPRLGQAGLVSVACWLLASAHCSVVYVIEFSGDISHSQGTNGTCYLEFRKDQLAILLPVRLEMAVVLFVVPLIITSYCYSRLVWILGRGGSHRRQRRVAGLLAATLLNFLVCFGPYNVSHVVGYICGESPAWRIYVTLLSTLNSCVDPFVYYFSSSGFQADFHELLRRLCGLWGQWQQESSMELKEQKGGEEQRADRPAERKTSEHSQGCGTGGQVACAES

Target ORF information:

RefSeq Version	NM_005304.5
Organism	Homo sapiens(human)
Definition	Homo sapiens free fatty acid receptor 3 (FFAR3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005304.5

ORF Insert Sequence:

ATGGATACAG GCCCCGACCA GTCCTACTTC TCCGGCAATC ACTGGTTCGT CTTCTCGGTG 
TACCTTCTCA CTTTCCTGGT GGGGCTCCCC CTCAACCTGC TGGCCCTGGT GGTCTTCGTG 
GGCAAGCTGC AGCGCCGCCC GGTGGCCGTG GACGTGCTCC TGCTCAACCT GACCGCCTCG 
GACCTGCTCC TGCTGCTGTT CCTGCCTTTC CGCATGGTGG AGGCAGCCAA TGGCATGCAC 
TGGCCCCTGC CCTTCATCCT CTGCCCACTC TCTGGATTCA TCTTCTTCAC CACCATCTAT 
CTCACCGCCC TCTTCCTGGC AGCTGTGAGC ATTGAACGCT TCCTGAGTGT GGCCCACCCA 
CTGTGGTACA AGACCCGGCC GAGGCTGGGG CAGGCAGGTC TGGTGAGTGT GGCCTGCTGG 
CTGTTGGCCT CTGCTCACTG CAGCGTGGTC TACGTCATAG AATTCTCAGG GGACATCTCC 
CACAGCCAGG GCACCAATGG GACCTGCTAC CTGGAGTTCC GGAAGGACCA GCTAGCCATC 
CTCCTGCCCG TGCGGCTGGA GATGGCTGTG GTCCTCTTTG TGGTCCCGCT GATCATCACC 
AGCTACTGCT ACAGCCGCCT GGTGTGGATC CTCGGCAGAG GGGGCAGCCA CCGCCGGCAG 
AGGAGGGTGG CGGGGCTGTT GGCGGCCACG CTGCTCAACT TCCTTGTCTG CTTTGGGCCC 
TACAACGTGT CCCATGTCGT GGGCTATATC TGCGGTGAAA GCCCGGCGTG GAGGATCTAC 
GTGACGCTTC TCAGCACCCT GAACTCCTGT GTCGACCCCT TTGTCTACTA CTTCTCCTCC 
TCCGGGTTCC AAGCCGACTT TCATGAGCTG CTGAGGAGGT TGTGTGGGCT CTGGGGCCAG 
TGGCAGCAGG AGAGCAGCAT GGAGCTGAAG GAGCAGAAGG GAGGGGAGGA GCAGAGAGCG 
GACCGACCAG CTGAAAGAAA GACCAGTGAA CACTCACAGG GCTGTGGAAC TGGTGGCCAG 
GTGGCCTGTG CTGAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FFAR3 ( XM_011526858.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525160.1 Homo sapiens free fatty acid receptor 3 (FFAR3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu58029
Clone ID Related Accession (Same CDS sequence)	XM_011526858.2
Accession Version	XM_011526858.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	free fatty acid receptor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526858.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGG AGGCACGCAG TTGTGGAATT TGTTCCCCTT TTAGCATGCT GACCAGCCCT 
GGCAACGGAG CTCAAGGCAT CTATGTGCCA CTGCTCAACA TGGCCACCAC CATGGATACA 
GGCCCCGACC AGTCCTACTT CTCCGGCAAT CACTGGTTCG TCTTCTCGGT GTACCTTCTC 
ACTTTCCTGG TGGGGCTCCC CCTCAACCTG CTGGCCCTGG TGGTCTTCGT GGGCAAGCTG 
CAGCGCCGCC CGGTGGCCGT GGACGTGCTC CTGCTCAACC TGACCGCCTC GGACCTGCTC 
CTGCTGCTGT TCCTGCCTTT CCGCATGGTG GAGGCAGCCA ATGGCATGCA CTGGCCCCTG 
CCCTTCATCC TCTGCCCACT CTCTGGATTC ATCTTCTTCA CCACCATCTA TCTCACCGCC 
CTCTTCCTGG CAGCTGTGAG CATTGAACGC TTCCTGAGTG TGGCCCACCC ACTGTGGTAC 
AAGACCCGGC CGAGGCTGGG GCAGGCAGGT CTGGTGAGTG TGGCCTGCTG GCTGTTGGCC 
TCTGCTCACT GCAGCGTGGT CTACGTCATA GAATTCTCAG GGGACATCTC CCACAGCCAG 
GGCACCAATG GGACCTGCTA CCTGGAGTTC CGGAAGGACC AGCTAGCCAT CCTCCTGCCC 
GTGCGGCTGG AGATGGCTGT GGTCCTCTTT GTGGTCCCGC TGATCATCAC CAGCTACTGC 
TACAGCCGCC TGGTGTGGAT CCTCGGCAGA GGGGGCAGCC ACCGCCGGCA GAGGAGGGTG 
GCGGGGCTGT TGGCGGCCAC GCTGCTCAAC TTCCTTGTCT GCTTTGGGCC CTACAACGTG 
TCCCATGTCG TGGGCTATAT CTGCGGTGAA AGCCCGGCGT GGAGGATCTA CGTGACGCTT 
CTCAGCACCC TGAACTCCTG TGTCGACCCC TTTGTCTACT ACTTCTCCTC CTCCGGGTTC 
CAAGCCGACT TTCATGAGCT GCTGAGGAGG TTGTGTGGGC TCTGGGGCCA GTGGCAGCAG 
GAGAGCAGCA TGGAGCTGAA GGAGCAGAAG GGAGGGGAGG AGCAGAGAGC GGACCGACCA 
GCTGAAAGAA AGACCAGTGA ACACTCACAG GGCTGTGGAA CTGGTGGCCA GGTGGCCTGT 
GCTGAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525160.1
CDS	321..1472
Translation	MRREARSCGICSPFSMLTSPGNGAQGIYVPLLNMATTMDTGPDQSYFSGNHWFVFSVYLLTFLVGLPLNLLALVVFVGKLQRRPVAVDVLLLNLTASDLLLLLFLPFRMVEAANGMHWPLPFILCPLSGFIFFTTIYLTALFLAAVSIERFLSVAHPLWYKTRPRLGQAGLVSVACWLLASAHCSVVYVIEFSGDISHSQGTNGTCYLEFRKDQLAILLPVRLEMAVVLFVVPLIITSYCYSRLVWILGRGGSHRRQRRVAGLLAATLLNFLVCFGPYNVSHVVGYICGESPAWRIYVTLLSTLNSCVDPFVYYFSSSGFQADFHELLRRLCGLWGQWQQESSMELKEQKGGEEQRADRPAERKTSEHSQGCGTGGQVACAES

Target ORF information:

RefSeq Version	XM_011526858.2
Organism	Homo sapiens(human)
Definition	Homo sapiens free fatty acid receptor 3 (FFAR3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011526858.2

ORF Insert Sequence:

ATGAGGAGGG AGGCACGCAG TTGTGGAATT TGTTCCCCTT TTAGCATGCT GACCAGCCCT 
GGCAACGGAG CTCAAGGCAT CTATGTGCCA CTGCTCAACA TGGCCACCAC CATGGATACA 
GGCCCCGACC AGTCCTACTT CTCCGGCAAT CACTGGTTCG TCTTCTCGGT GTACCTTCTC 
ACTTTCCTGG TGGGGCTCCC CCTCAACCTG CTGGCCCTGG TGGTCTTCGT GGGCAAGCTG 
CAGCGCCGCC CGGTGGCCGT GGACGTGCTC CTGCTCAACC TGACCGCCTC GGACCTGCTC 
CTGCTGCTGT TCCTGCCTTT CCGCATGGTG GAGGCAGCCA ATGGCATGCA CTGGCCCCTG 
CCCTTCATCC TCTGCCCACT CTCTGGATTC ATCTTCTTCA CCACCATCTA TCTCACCGCC 
CTCTTCCTGG CAGCTGTGAG CATTGAACGC TTCCTGAGTG TGGCCCACCC ACTGTGGTAC 
AAGACCCGGC CGAGGCTGGG GCAGGCAGGT CTGGTGAGTG TGGCCTGCTG GCTGTTGGCC 
TCTGCTCACT GCAGCGTGGT CTACGTCATA GAATTCTCAG GGGACATCTC CCACAGCCAG 
GGCACCAATG GGACCTGCTA CCTGGAGTTC CGGAAGGACC AGCTAGCCAT CCTCCTGCCC 
GTGCGGCTGG AGATGGCTGT GGTCCTCTTT GTGGTCCCGC TGATCATCAC CAGCTACTGC 
TACAGCCGCC TGGTGTGGAT CCTCGGCAGA GGGGGCAGCC ACCGCCGGCA GAGGAGGGTG 
GCGGGGCTGT TGGCGGCCAC GCTGCTCAAC TTCCTTGTCT GCTTTGGGCC CTACAACGTG 
TCCCATGTCG TGGGCTATAT CTGCGGTGAA AGCCCGGCGT GGAGGATCTA CGTGACGCTT 
CTCAGCACCC TGAACTCCTG TGTCGACCCC TTTGTCTACT ACTTCTCCTC CTCCGGGTTC 
CAAGCCGACT TTCATGAGCT GCTGAGGAGG TTGTGTGGGC TCTGGGGCCA GTGGCAGCAG 
GAGAGCAGCA TGGAGCTGAA GGAGCAGAAG GGAGGGGAGG AGCAGAGAGC GGACCGACCA 
GCTGAAAGAA AGACCAGTGA ACACTCACAG GGCTGTGGAA CTGGTGGCCA GGTGGCCTGT 
GCTGAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FFAR2-FFAR3 receptor heteromerization modulates short-chain fatty acid sensing.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology32(1)289-303(2018 Jan)
Ang Z,Xiong D,Wu M,Ding JL

IL-1? and TNF? inhibit GPR120 (FFAR4) and stimulate GPR84 (EX33) and GPR41 (FFAR3) fatty acid receptor expression in human adipocytes: implications for the anti-inflammatory action of n-3 fatty acids.
Archives of physiology and biochemistry1-12(2017 Aug)
Muredda L,K?pczy?ska MA,Zaibi MS,Alomar SY,Trayhurn P

Short-chain fatty acids, GPR41 and GPR43 ligands, inhibit TNF-?-induced MCP-1 expression by modulating p38 and JNK signaling pathways in human renal cortical epithelial cells.
Biochemical and biophysical research communications486(2)499-505(2017 04)
Kobayashi M,Mikami D,Kimura H,Kamiyama K,Morikawa Y,Yokoi S,Kasuno K,Takahashi N,Taniguchi T,Iwano M

Soluble Fibre Meal Challenge Reduces Airway Inflammation and Expression of GPR43 and GPR41 in Asthma.
Nutrients9(1)(2017 Jan)
Halnes I,Baines KJ,Berthon BS,MacDonald-Wicks LK,Gibson PG,Wood LG

Human GPR42 is a transcribed multisite variant that exhibits copy number polymorphism and is functional when heterologously expressed.
Scientific reports512880(2015 Aug)
Puhl HL 3rd,Won YJ,Lu VB,Ikeda SR