GPLD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPLD1
Entrez Gene ID	2822
Full Name	glycosylphosphatidylinositol specific phospholipase D1
Synonyms	GPIPLD,GPIPLDM,PIGPLD,PIGPLD1,PLD
General protein information	Preferred Names glycosylphosphatidylinositol specific phospholipase D1 Names phosphatidylinositol-glycan-specific phospholipase D GPI-PLD GPI-specific phospholipase D PI-G PLD glycoprotein phospholipase D
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p22.3
Summary	Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq, Jul 2008].
Disorder MIM:	602515

mRNA and Protein(s)

mRNA	Protein	Name
XM_017010754.1	XP_016866243.1	phosphatidylinositol-glycan-specific phospholipase D isoform X2
NM_001503.3	NP_001494.2	phosphatidylinositol-glycan-specific phospholipase D precursor
NM_001503.4	NP_001494.2	phosphatidylinositol-glycan-specific phospholipase D precursor
XM_017010753.1	XP_016866242.1	phosphatidylinositol-glycan-specific phospholipase D isoform X1
XM_011514509.2	XP_011512811.1	phosphatidylinositol-glycan-specific phospholipase D isoform X3
XM_024446403.1	XP_024302171.1	phosphatidylinositol-glycan-specific phospholipase D isoform X2
XM_017010753.2	XP_016866242.1	phosphatidylinositol-glycan-specific phospholipase D isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GPLD1	XP_518268.4
Xenopus tropicalis (tropical clawed frog)	LOC100493339	XP_002937968.2
Homo sapiens (human)	GPLD1	NP_001494.2
	GPLD1	XP_002803682.1
Canis lupus familiaris (dog)	GPLD1	XP_535902.2
Bos taurus (cattle)	GPLD1	NP_777241.1
Mus musculus (house mouse)	Gpld1	NP_032182.2
Rattus norvegicus (Norway rat)	Gpld1	NP_001093982.1
Gallus gallus (chicken)	GPLD1	XP_418910.4

Gene Ontology
Bibliography

Related articles in PubMed

Role of curcumin in PLD activation by Arf6-cytohesin1 signaling axis in U46619-stimulated pulmonary artery smooth muscle cells.
Chakraborti S, Sarkar J, Bhuyan R, Chakraborti T
Molecular and cellular biochemistry438(1-2)97-109(2018 Jan)

Regulation of glycosylphosphatidylinositol-anchored proteins and GPI-phospholipase D in a c-Myc transgenic mouse model of hepatocellular carcinoma and human HCC.
Ritorto MS, Rhode H, Vogel A, Borlak J
Biological chemistry397(11)1147-1162(2016 Nov)

MASP1, THBS1, GPLD1 and ApoA-IV are novel biomarkers associated with prediabetes: the KORA F4 study.
von Toerne C, Huth C, de Las Heras Gala T, Kronenberg F, Herder C, Koenig W, Meisinger C, Rathmann W, Waldenberger M, Roden M, Peters A, Thorand B, Hauck SM
Diabetologia59(9)1882-92(2016 Sep)

Down-regulation of MicroRNAs (MiRs) 203, 887, 3619 and 182 Prevents Vimentin-triggered, Phospholipase D (PLD)-mediated Cancer Cell Invasion.
Fite K, Gomez-Cambronero J
The Journal of biological chemistry291(2)719-30(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

c-Myc influences GPI-AP signaling transcriptionally and posttranslational and represses GPI-AP anti-proliferative signaling in tumors
Title: Regulation of glycosylphosphatidylinositol-anchored proteins and GPI-phospholipase D in a c-Myc transgenic mouse model of hepatocellular carcinoma and human HCC.

Study discovered novel and independent associations of prediabetes and related traits with MASP1, and some evidence for associations with THBS1, GPLD1 and ApoA-IV, suggesting a role for these proteins in the pathophysiology of type 2 diabetes.
Title: MASP1, THBS1, GPLD1 and ApoA-IV are novel biomarkers associated with prediabetes: the KORA F4 study.

Low aggressive MCF-7 breast cancer cells have low endogenous PLD enzymatic activity and cell invasion, concomitant with high expression of miR-203, -887, and -3619 and miR-182 and miR-182.
Title: Down-regulation of MicroRNAs (MiRs) 203, 887, 3619 and 182 Prevents Vimentin-triggered, Phospholipase D (PLD)-mediated Cancer Cell Invasion.

An observed increase in PLD activity was mediated through boosting the binding of PLD with dynamin which in turn facilitated fibronectin-induced cell spreading.
Title: GTP-dependent interaction between phospholipase D and dynamin modulates fibronectin-induced cell spreading.

our findings showed that ANRIL is an lncRNA responsible in anti-tumorigenesis caused by PLD inhibition and combined incorporation of ANRIL into PLD inhibition-induced anti-tumorigenic signaling network
Title: Down-regulation of Phospholipase D Stimulates Death of Lung Cancer Cells Involving Up-regulation of the Long ncRNA ANRIL.

The following GPLD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPLD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21813	NM_001503.4 Latest version!	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu58020	XM_011514509.2 Latest version!	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu40267	XM_017010754.1 Latest version!	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu40267	XM_024446403.1 Latest version!	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu96547	XM_017010753.2 Latest version!	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu21813	NM_001503.3	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu96547	XM_017010753.1	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPLD1 ( NM_001503.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001494.2 Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), mRNA.

Price & Availability↑

CloneID	OHu21813
Clone ID Related Accession (Same CDS sequence)	NM_001503.3 , NM_001503.4
Accession Version	NM_001503.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan-specific phospholipase D precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ308108.1, AL031230.1, L11702.1, AY007546.2, AL359713.25 and BU587813.1. This sequence is a reference standard in the RefSeqGene project. On Apr 26, 2012 this sequence version replaced NM_001503.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ308108.1, L11702.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGCTT TCAGGTTGTG GCCTGGCCTG CTGATCATGT TGGGTTCTCT CTGCCATAGA 
GGTTCACCGT GTGGCCTTTC AACACACGTA GAAATAGGAC ACAGAGCTCT GGAGTTTCTT 
CAGCTTCACA ATGGGCGTGT TAACTACAGA GAGCTGTTAC TAGAACACCA GGATGCGTAT 
CAGGCTGGAA TCGTGTTTCC TGATTGTTTT TACCCTAGCA TCTGCAAAGG AGGAAAATTC 
CATGATGTGT CTGAGAGCAC TCACTGGACT CCGTTTCTTA ATGCAAGCGT TCATTATATC 
CGAGAGAACT ATCCCCTTCC CTGGGAGAAG GACACAGAGA AACTGGTAGC TTTCTTGTTT 
GGAATTACTT CTCACATGGC GGCAGATGTC AGCTGGCATA GTCTGGGCCT TGAACAAGGA 
TTCCTTAGGA CCATGGGAGC TATTGATTTT CACGGCTCCT ATTCAGAGGC TCATTCGGCT 
GGTGATTTTG GAGGAGATGT GTTGAGCCAG TTTGAATTTA ATTTTAATTA CCTTGCACGA 
CGCTGGTATG TGCCAGTCAA AGATCTACTG GGAATTTATG AGAAACTGTA TGGTCGAAAA 
GTCATCACCG AAAATGTAAT CGTTGATTGT TCACATATCC AGTTCTTAGA AATGTATGGT 
GAGATGCTAG CTGTTTCCAA GTTATATCCC ACTTACTCTA CAAAGTCCCC GTTTTTGGTG 
GAACAATTCC AAGAGTATTT TCTTGGAGGA CTGGATGATA TGGCATTTTG GTCCACTAAT 
ATTTACCATC TAACAAGCTT CATGTTGGAG AATGGGACCA GTGACTGCAA CCTGCCTGAG 
AACCCTCTGT TCATTGCATG TGGCGGCCAG CAAAACCACA CCCAGGGCTC AAAAATGCAG 
AAAAATGATT TTCACAGAAA TTTGACTACA TCCCTAACTG AAAGTGTTGA CAGGAATATA 
AACTATACTG AAAGAGGAGT GTTCTTTAGT GTAAATTCCT GGACCCCGGA TTCCATGTCC 
TTTATCTACA AGGCTTTGGA AAGGAACATA AGGACAATGT TCATAGGTGG CTCTCAGTTG 
TCACAAAAGC ACGTCTCCAG CCCCTTAGCA TCTTACTTCT TGTCATTTCC TTATGCGAGG 
CTTGGCTGGG CAATGACCTC AGCTGACCTC AACCAGGATG GGCACGGTGA CCTCGTGGTG 
GGCGCACCAG GCTACAGCCG CCCCGGCCAC ATCCACATCG GGCGCGTGTA CCTCATCTAC 
GGCAATGACC TGGGCCTGCC ACCTGTTGAC CTGGACCTGG ACAAGGAGGC CCACAGGATC 
CTTGAAGGCT TCCAGCCCTC AGGTCGGTTT GGCTCGGCCT TGGCTGTGTT GGACTTTAAC 
GTGGACGGCG TGCCTGACCT GGCCGTGGGA GCTCCCTCGG TGGGCTCCGA GCAGCTCACC 
TACAAAGGTG CCGTGTATGT CTACTTTGGT TCCAAACAAG GAGGAATGTC TTCTTCCCCT 
AACATCACCA TTTCTTGCCA GGACATCTAC TGTAACTTGG GCTGGACTCT CTTGGCTGCA 
GATGTGAATG GAGACAGTGA ACCCGATCTG GTCATCGGCT CCCCTTTTGC ACCAGGTGGA 
GGGAAGCAGA AGGGAATTGT GGCTGCGTTT TATTCTGGCC CCAGCCTGAG CGACAAAGAA 
AAACTGAACG TGGAGGCAGC CAACTGGACG GTGAGAGGCG AGGAAGACTT CTCCTGGTTT 
GGATATTCCC TTCACGGTGT CACTGTGGAC AACAGAACCT TGCTGTTGGT TGGGAGCCCG 
ACCTGGAAGA ATGCCAGCAG GCTGGGCCAT TTGTTACACA TCCGAGATGA GAAAAAGAGC 
CTTGGGAGGG TGTATGGCTA CTTCCCACCA AACGGCCAAA GCTGGTTTAC CATTTCTGGA 
GACAAGGCAA TGGGGAAACT GGGTACTTCC CTTTCCAGTG GCCACGTACT GATGAATGGG 
ACTCTGAAAC AAGTGCTGCT GGTTGGAGCC CCTACGTACG ATGACGTGTC TAAGGTGGCA 
TTCCTGACCG TGACCCTACA CCAAGGCGGA GCCACTCGCA TGTACGCACT CACATCTGAC 
GCGCAGCCTC TGCTGCTCAG CACCTTCAGC GGAGACCGCC GCTTCTCCCG ATTTGGTGGC 
GTTCTGCACT TGAGTGACCT GGATGATGAT GGCTTAGATG AAATCATCAT GGCAGCCCCC 
CTGAGGATAG CAGATGTAAC CTCTGGACTG ATTGGGGGAG AAGACGGCCG AGTATATGTA 
TATAATGGCA AAGAGACCAC CCTTGGTGAC ATGACTGGCA AATGCAAATC ATGGATAACT 
CCATGTCCAG AAGAAAAGGC CCAATATGTA TTGATTTCTC CTGAAGCCAG CTCAAGGTTT 
GGGAGCTCCC TCATCACCGT GAGGTCCAAG GCAAAGAACC AAGTCGTCAT TGCTGCTGGA 
AGGAGTTCTT TGGGAGCCCG ACTCTCCGGG GCACTTCACG TCTATAGCCT TGGCTCAGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001494.2
CDS	112..2634
Translation	MSAFRLWPGLLIMLGSLCHRGSPCGLSTHVEIGHRALEFLQLHNGRVNYRELLLEHQDAYQAGIVFPDCFYPSICKGGKFHDVSESTHWTPFLNASVHYIRENYPLPWEKDTEKLVAFLFGITSHMAADVSWHSLGLEQGFLRTMGAIDFHGSYSEAHSAGDFGGDVLSQFEFNFNYLARRWYVPVKDLLGIYEKLYGRKVITENVIVDCSHIQFLEMYGEMLAVSKLYPTYSTKSPFLVEQFQEYFLGGLDDMAFWSTNIYHLTSFMLENGTSDCNLPENPLFIACGGQQNHTQGSKMQKNDFHRNLTTSLTESVDRNINYTERGVFFSVNSWTPDSMSFIYKALERNIRTMFIGGSQLSQKHVSSPLASYFLSFPYARLGWAMTSADLNQDGHGDLVVGAPGYSRPGHIHIGRVYLIYGNDLGLPPVDLDLDKEAHRILEGFQPSGRFGSALAVLDFNVDGVPDLAVGAPSVGSEQLTYKGAVYVYFGSKQGGMSSSPNITISCQDIYCNLGWTLLAADVNGDSEPDLVIGSPFAPGGGKQKGIVAAFYSGPSLSDKEKLNVEAANWTVRGEEDFSWFGYSLHGVTVDNRTLLLVGSPTWKNASRLGHLLHIRDEKKSLGRVYGYFPPNGQSWFTISGDKAMGKLGTSLSSGHVLMNGTLKQVLLVGAPTYDDVSKVAFLTVTLHQGGATRMYALTSDAQPLLLSTFSGDRRFSRFGGVLHLSDLDDDGLDEIIMAAPLRIADVTSGLIGGEDGRVYVYNGKETTLGDMTGKCKSWITPCPEEKAQYVLISPEASSRFGSSLITVRSKAKNQVVIAAGRSSLGARLSGALHVYSLGSD

Target ORF information:

RefSeq Version	NM_001503.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001503.3

ORF Insert Sequence:

ATGTCTGCTT TCAGGTTGTG GCCTGGCCTG CTGATCATGT TGGGTTCTCT CTGCCATAGA 
GGTTCACCGT GTGGCCTTTC AACACACGTA GAAATAGGAC ACAGAGCTCT GGAGTTTCTT 
CAGCTTCACA ATGGGCGTGT TAACTACAGA GAGCTGTTAC TAGAACACCA GGATGCGTAT 
CAGGCTGGAA TCGTGTTTCC TGATTGTTTT TACCCTAGCA TCTGCAAAGG AGGAAAATTC 
CATGATGTGT CTGAGAGCAC TCACTGGACT CCGTTTCTTA ATGCAAGCGT TCATTATATC 
CGAGAGAACT ATCCCCTTCC CTGGGAGAAG GACACAGAGA AACTGGTAGC TTTCTTGTTT 
GGAATTACTT CTCACATGGC GGCAGATGTC AGCTGGCATA GTCTGGGCCT TGAACAAGGA 
TTCCTTAGGA CCATGGGAGC TATTGATTTT CACGGCTCCT ATTCAGAGGC TCATTCGGCT 
GGTGATTTTG GAGGAGATGT GTTGAGCCAG TTTGAATTTA ATTTTAATTA CCTTGCACGA 
CGCTGGTATG TGCCAGTCAA AGATCTACTG GGAATTTATG AGAAACTGTA TGGTCGAAAA 
GTCATCACCG AAAATGTAAT CGTTGATTGT TCACATATCC AGTTCTTAGA AATGTATGGT 
GAGATGCTAG CTGTTTCCAA GTTATATCCC ACTTACTCTA CAAAGTCCCC GTTTTTGGTG 
GAACAATTCC AAGAGTATTT TCTTGGAGGA CTGGATGATA TGGCATTTTG GTCCACTAAT 
ATTTACCATC TAACAAGCTT CATGTTGGAG AATGGGACCA GTGACTGCAA CCTGCCTGAG 
AACCCTCTGT TCATTGCATG TGGCGGCCAG CAAAACCACA CCCAGGGCTC AAAAATGCAG 
AAAAATGATT TTCACAGAAA TTTGACTACA TCCCTAACTG AAAGTGTTGA CAGGAATATA 
AACTATACTG AAAGAGGAGT GTTCTTTAGT GTAAATTCCT GGACCCCGGA TTCCATGTCC 
TTTATCTACA AGGCTTTGGA AAGGAACATA AGGACAATGT TCATAGGTGG CTCTCAGTTG 
TCACAAAAGC ACGTCTCCAG CCCCTTAGCA TCTTACTTCT TGTCATTTCC TTATGCGAGG 
CTTGGCTGGG CAATGACCTC AGCTGACCTC AACCAGGATG GGCACGGTGA CCTCGTGGTG 
GGCGCACCAG GCTACAGCCG CCCCGGCCAC ATCCACATCG GGCGCGTGTA CCTCATCTAC 
GGCAATGACC TGGGCCTGCC ACCTGTTGAC CTGGACCTGG ACAAGGAGGC CCACAGGATC 
CTTGAAGGCT TCCAGCCCTC AGGTCGGTTT GGCTCGGCCT TGGCTGTGTT GGACTTTAAC 
GTGGACGGCG TGCCTGACCT GGCCGTGGGA GCTCCCTCGG TGGGCTCCGA GCAGCTCACC 
TACAAAGGTG CCGTGTATGT CTACTTTGGT TCCAAACAAG GAGGAATGTC TTCTTCCCCT 
AACATCACCA TTTCTTGCCA GGACATCTAC TGTAACTTGG GCTGGACTCT CTTGGCTGCA 
GATGTGAATG GAGACAGTGA ACCCGATCTG GTCATCGGCT CCCCTTTTGC ACCAGGTGGA 
GGGAAGCAGA AGGGAATTGT GGCTGCGTTT TATTCTGGCC CCAGCCTGAG CGACAAAGAA 
AAACTGAACG TGGAGGCAGC CAACTGGACG GTGAGAGGCG AGGAAGACTT CTCCTGGTTT 
GGATATTCCC TTCACGGTGT CACTGTGGAC AACAGAACCT TGCTGTTGGT TGGGAGCCCG 
ACCTGGAAGA ATGCCAGCAG GCTGGGCCAT TTGTTACACA TCCGAGATGA GAAAAAGAGC 
CTTGGGAGGG TGTATGGCTA CTTCCCACCA AACGGCCAAA GCTGGTTTAC CATTTCTGGA 
GACAAGGCAA TGGGGAAACT GGGTACTTCC CTTTCCAGTG GCCACGTACT GATGAATGGG 
ACTCTGAAAC AAGTGCTGCT GGTTGGAGCC CCTACGTACG ATGACGTGTC TAAGGTGGCA 
TTCCTGACCG TGACCCTACA CCAAGGCGGA GCCACTCGCA TGTACGCACT CACATCTGAC 
GCGCAGCCTC TGCTGCTCAG CACCTTCAGC GGAGACCGCC GCTTCTCCCG ATTTGGTGGC 
GTTCTGCACT TGAGTGACCT GGATGATGAT GGCTTAGATG AAATCATCAT GGCAGCCCCC 
CTGAGGATAG CAGATGTAAC CTCTGGACTG ATTGGGGGAG AAGACGGCCG AGTATATGTA 
TATAATGGCA AAGAGACCAC CCTTGGTGAC ATGACTGGCA AATGCAAATC ATGGATAACT 
CCATGTCCAG AAGAAAAGGC CCAATATGTA TTGATTTCTC CTGAAGCCAG CTCAAGGTTT 
GGGAGCTCCC TCATCACCGT GAGGTCCAAG GCAAAGAACC AAGTCGTCAT TGCTGCTGGA 
AGGAGTTCTT TGGGAGCCCG ACTCTCCGGG GCACTTCACG TCTATAGCCT TGGCTCAGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPLD1 ( NM_001503.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001494.2 Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), mRNA.

Price & Availability↑

CloneID	OHu21813
Clone ID Related Accession (Same CDS sequence)	NM_001503.3 , NM_001503.4
Accession Version	NM_001503.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan-specific phospholipase D precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ308108.1, AL031230.1, L11702.1, AY007546.2, AL359713.25 and BU587813.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001503.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L11702.1, AJ308108.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000230036.2/ ENSP00000230036.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGCTT TCAGGTTGTG GCCTGGCCTG CTGATCATGT TGGGTTCTCT CTGCCATAGA 
GGTTCACCGT GTGGCCTTTC AACACACGTA GAAATAGGAC ACAGAGCTCT GGAGTTTCTT 
CAGCTTCACA ATGGGCGTGT TAACTACAGA GAGCTGTTAC TAGAACACCA GGATGCGTAT 
CAGGCTGGAA TCGTGTTTCC TGATTGTTTT TACCCTAGCA TCTGCAAAGG AGGAAAATTC 
CATGATGTGT CTGAGAGCAC TCACTGGACT CCGTTTCTTA ATGCAAGCGT TCATTATATC 
CGAGAGAACT ATCCCCTTCC CTGGGAGAAG GACACAGAGA AACTGGTAGC TTTCTTGTTT 
GGAATTACTT CTCACATGGC GGCAGATGTC AGCTGGCATA GTCTGGGCCT TGAACAAGGA 
TTCCTTAGGA CCATGGGAGC TATTGATTTT CACGGCTCCT ATTCAGAGGC TCATTCGGCT 
GGTGATTTTG GAGGAGATGT GTTGAGCCAG TTTGAATTTA ATTTTAATTA CCTTGCACGA 
CGCTGGTATG TGCCAGTCAA AGATCTACTG GGAATTTATG AGAAACTGTA TGGTCGAAAA 
GTCATCACCG AAAATGTAAT CGTTGATTGT TCACATATCC AGTTCTTAGA AATGTATGGT 
GAGATGCTAG CTGTTTCCAA GTTATATCCC ACTTACTCTA CAAAGTCCCC GTTTTTGGTG 
GAACAATTCC AAGAGTATTT TCTTGGAGGA CTGGATGATA TGGCATTTTG GTCCACTAAT 
ATTTACCATC TAACAAGCTT CATGTTGGAG AATGGGACCA GTGACTGCAA CCTGCCTGAG 
AACCCTCTGT TCATTGCATG TGGCGGCCAG CAAAACCACA CCCAGGGCTC AAAAATGCAG 
AAAAATGATT TTCACAGAAA TTTGACTACA TCCCTAACTG AAAGTGTTGA CAGGAATATA 
AACTATACTG AAAGAGGAGT GTTCTTTAGT GTAAATTCCT GGACCCCGGA TTCCATGTCC 
TTTATCTACA AGGCTTTGGA AAGGAACATA AGGACAATGT TCATAGGTGG CTCTCAGTTG 
TCACAAAAGC ACGTCTCCAG CCCCTTAGCA TCTTACTTCT TGTCATTTCC TTATGCGAGG 
CTTGGCTGGG CAATGACCTC AGCTGACCTC AACCAGGATG GGCACGGTGA CCTCGTGGTG 
GGCGCACCAG GCTACAGCCG CCCCGGCCAC ATCCACATCG GGCGCGTGTA CCTCATCTAC 
GGCAATGACC TGGGCCTGCC ACCTGTTGAC CTGGACCTGG ACAAGGAGGC CCACAGGATC 
CTTGAAGGCT TCCAGCCCTC AGGTCGGTTT GGCTCGGCCT TGGCTGTGTT GGACTTTAAC 
GTGGACGGCG TGCCTGACCT GGCCGTGGGA GCTCCCTCGG TGGGCTCCGA GCAGCTCACC 
TACAAAGGTG CCGTGTATGT CTACTTTGGT TCCAAACAAG GAGGAATGTC TTCTTCCCCT 
AACATCACCA TTTCTTGCCA GGACATCTAC TGTAACTTGG GCTGGACTCT CTTGGCTGCA 
GATGTGAATG GAGACAGTGA ACCCGATCTG GTCATCGGCT CCCCTTTTGC ACCAGGTGGA 
GGGAAGCAGA AGGGAATTGT GGCTGCGTTT TATTCTGGCC CCAGCCTGAG CGACAAAGAA 
AAACTGAACG TGGAGGCAGC CAACTGGACG GTGAGAGGCG AGGAAGACTT CTCCTGGTTT 
GGATATTCCC TTCACGGTGT CACTGTGGAC AACAGAACCT TGCTGTTGGT TGGGAGCCCG 
ACCTGGAAGA ATGCCAGCAG GCTGGGCCAT TTGTTACACA TCCGAGATGA GAAAAAGAGC 
CTTGGGAGGG TGTATGGCTA CTTCCCACCA AACGGCCAAA GCTGGTTTAC CATTTCTGGA 
GACAAGGCAA TGGGGAAACT GGGTACTTCC CTTTCCAGTG GCCACGTACT GATGAATGGG 
ACTCTGAAAC AAGTGCTGCT GGTTGGAGCC CCTACGTACG ATGACGTGTC TAAGGTGGCA 
TTCCTGACCG TGACCCTACA CCAAGGCGGA GCCACTCGCA TGTACGCACT CACATCTGAC 
GCGCAGCCTC TGCTGCTCAG CACCTTCAGC GGAGACCGCC GCTTCTCCCG ATTTGGTGGC 
GTTCTGCACT TGAGTGACCT GGATGATGAT GGCTTAGATG AAATCATCAT GGCAGCCCCC 
CTGAGGATAG CAGATGTAAC CTCTGGACTG ATTGGGGGAG AAGACGGCCG AGTATATGTA 
TATAATGGCA AAGAGACCAC CCTTGGTGAC ATGACTGGCA AATGCAAATC ATGGATAACT 
CCATGTCCAG AAGAAAAGGC CCAATATGTA TTGATTTCTC CTGAAGCCAG CTCAAGGTTT 
GGGAGCTCCC TCATCACCGT GAGGTCCAAG GCAAAGAACC AAGTCGTCAT TGCTGCTGGA 
AGGAGTTCTT TGGGAGCCCG ACTCTCCGGG GCACTTCACG TCTATAGCCT TGGCTCAGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001494.2
CDS	68..2590
Translation	MSAFRLWPGLLIMLGSLCHRGSPCGLSTHVEIGHRALEFLQLHNGRVNYRELLLEHQDAYQAGIVFPDCFYPSICKGGKFHDVSESTHWTPFLNASVHYIRENYPLPWEKDTEKLVAFLFGITSHMAADVSWHSLGLEQGFLRTMGAIDFHGSYSEAHSAGDFGGDVLSQFEFNFNYLARRWYVPVKDLLGIYEKLYGRKVITENVIVDCSHIQFLEMYGEMLAVSKLYPTYSTKSPFLVEQFQEYFLGGLDDMAFWSTNIYHLTSFMLENGTSDCNLPENPLFIACGGQQNHTQGSKMQKNDFHRNLTTSLTESVDRNINYTERGVFFSVNSWTPDSMSFIYKALERNIRTMFIGGSQLSQKHVSSPLASYFLSFPYARLGWAMTSADLNQDGHGDLVVGAPGYSRPGHIHIGRVYLIYGNDLGLPPVDLDLDKEAHRILEGFQPSGRFGSALAVLDFNVDGVPDLAVGAPSVGSEQLTYKGAVYVYFGSKQGGMSSSPNITISCQDIYCNLGWTLLAADVNGDSEPDLVIGSPFAPGGGKQKGIVAAFYSGPSLSDKEKLNVEAANWTVRGEEDFSWFGYSLHGVTVDNRTLLLVGSPTWKNASRLGHLLHIRDEKKSLGRVYGYFPPNGQSWFTISGDKAMGKLGTSLSSGHVLMNGTLKQVLLVGAPTYDDVSKVAFLTVTLHQGGATRMYALTSDAQPLLLSTFSGDRRFSRFGGVLHLSDLDDDGLDEIIMAAPLRIADVTSGLIGGEDGRVYVYNGKETTLGDMTGKCKSWITPCPEEKAQYVLISPEASSRFGSSLITVRSKAKNQVVIAAGRSSLGARLSGALHVYSLGSD

Target ORF information:

RefSeq Version	NM_001503.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001503.4

ORF Insert Sequence:

ATGTCTGCTT TCAGGTTGTG GCCTGGCCTG CTGATCATGT TGGGTTCTCT CTGCCATAGA 
GGTTCACCGT GTGGCCTTTC AACACACGTA GAAATAGGAC ACAGAGCTCT GGAGTTTCTT 
CAGCTTCACA ATGGGCGTGT TAACTACAGA GAGCTGTTAC TAGAACACCA GGATGCGTAT 
CAGGCTGGAA TCGTGTTTCC TGATTGTTTT TACCCTAGCA TCTGCAAAGG AGGAAAATTC 
CATGATGTGT CTGAGAGCAC TCACTGGACT CCGTTTCTTA ATGCAAGCGT TCATTATATC 
CGAGAGAACT ATCCCCTTCC CTGGGAGAAG GACACAGAGA AACTGGTAGC TTTCTTGTTT 
GGAATTACTT CTCACATGGC GGCAGATGTC AGCTGGCATA GTCTGGGCCT TGAACAAGGA 
TTCCTTAGGA CCATGGGAGC TATTGATTTT CACGGCTCCT ATTCAGAGGC TCATTCGGCT 
GGTGATTTTG GAGGAGATGT GTTGAGCCAG TTTGAATTTA ATTTTAATTA CCTTGCACGA 
CGCTGGTATG TGCCAGTCAA AGATCTACTG GGAATTTATG AGAAACTGTA TGGTCGAAAA 
GTCATCACCG AAAATGTAAT CGTTGATTGT TCACATATCC AGTTCTTAGA AATGTATGGT 
GAGATGCTAG CTGTTTCCAA GTTATATCCC ACTTACTCTA CAAAGTCCCC GTTTTTGGTG 
GAACAATTCC AAGAGTATTT TCTTGGAGGA CTGGATGATA TGGCATTTTG GTCCACTAAT 
ATTTACCATC TAACAAGCTT CATGTTGGAG AATGGGACCA GTGACTGCAA CCTGCCTGAG 
AACCCTCTGT TCATTGCATG TGGCGGCCAG CAAAACCACA CCCAGGGCTC AAAAATGCAG 
AAAAATGATT TTCACAGAAA TTTGACTACA TCCCTAACTG AAAGTGTTGA CAGGAATATA 
AACTATACTG AAAGAGGAGT GTTCTTTAGT GTAAATTCCT GGACCCCGGA TTCCATGTCC 
TTTATCTACA AGGCTTTGGA AAGGAACATA AGGACAATGT TCATAGGTGG CTCTCAGTTG 
TCACAAAAGC ACGTCTCCAG CCCCTTAGCA TCTTACTTCT TGTCATTTCC TTATGCGAGG 
CTTGGCTGGG CAATGACCTC AGCTGACCTC AACCAGGATG GGCACGGTGA CCTCGTGGTG 
GGCGCACCAG GCTACAGCCG CCCCGGCCAC ATCCACATCG GGCGCGTGTA CCTCATCTAC 
GGCAATGACC TGGGCCTGCC ACCTGTTGAC CTGGACCTGG ACAAGGAGGC CCACAGGATC 
CTTGAAGGCT TCCAGCCCTC AGGTCGGTTT GGCTCGGCCT TGGCTGTGTT GGACTTTAAC 
GTGGACGGCG TGCCTGACCT GGCCGTGGGA GCTCCCTCGG TGGGCTCCGA GCAGCTCACC 
TACAAAGGTG CCGTGTATGT CTACTTTGGT TCCAAACAAG GAGGAATGTC TTCTTCCCCT 
AACATCACCA TTTCTTGCCA GGACATCTAC TGTAACTTGG GCTGGACTCT CTTGGCTGCA 
GATGTGAATG GAGACAGTGA ACCCGATCTG GTCATCGGCT CCCCTTTTGC ACCAGGTGGA 
GGGAAGCAGA AGGGAATTGT GGCTGCGTTT TATTCTGGCC CCAGCCTGAG CGACAAAGAA 
AAACTGAACG TGGAGGCAGC CAACTGGACG GTGAGAGGCG AGGAAGACTT CTCCTGGTTT 
GGATATTCCC TTCACGGTGT CACTGTGGAC AACAGAACCT TGCTGTTGGT TGGGAGCCCG 
ACCTGGAAGA ATGCCAGCAG GCTGGGCCAT TTGTTACACA TCCGAGATGA GAAAAAGAGC 
CTTGGGAGGG TGTATGGCTA CTTCCCACCA AACGGCCAAA GCTGGTTTAC CATTTCTGGA 
GACAAGGCAA TGGGGAAACT GGGTACTTCC CTTTCCAGTG GCCACGTACT GATGAATGGG 
ACTCTGAAAC AAGTGCTGCT GGTTGGAGCC CCTACGTACG ATGACGTGTC TAAGGTGGCA 
TTCCTGACCG TGACCCTACA CCAAGGCGGA GCCACTCGCA TGTACGCACT CACATCTGAC 
GCGCAGCCTC TGCTGCTCAG CACCTTCAGC GGAGACCGCC GCTTCTCCCG ATTTGGTGGC 
GTTCTGCACT TGAGTGACCT GGATGATGAT GGCTTAGATG AAATCATCAT GGCAGCCCCC 
CTGAGGATAG CAGATGTAAC CTCTGGACTG ATTGGGGGAG AAGACGGCCG AGTATATGTA 
TATAATGGCA AAGAGACCAC CCTTGGTGAC ATGACTGGCA AATGCAAATC ATGGATAACT 
CCATGTCCAG AAGAAAAGGC CCAATATGTA TTGATTTCTC CTGAAGCCAG CTCAAGGTTT 
GGGAGCTCCC TCATCACCGT GAGGTCCAAG GCAAAGAACC AAGTCGTCAT TGCTGCTGGA 
AGGAGTTCTT TGGGAGCCCG ACTCTCCGGG GCACTTCACG TCTATAGCCT TGGCTCAGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPLD1 ( XM_011514509.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512811.1 Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X3, mRNA.

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CloneID	OHu58020
Clone ID Related Accession (Same CDS sequence)	XM_011514509.2
Accession Version	XM_011514509.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan-specific phospholipase D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514509.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTCG CCATGGCCCG GGCAACGACG GCGACAGGAA ACAGGTTGTG GCCTGGCCTG 
CTGATCATGT TGGGTTCTCT CTGCCATAGA GGTTCACCGT GTGGCCTTTC AACACACGTA 
GAAATAGGAC ACAGAGCTCT GGAGTTTCTT CAGCTTCACA ATGGGCGTGT TAACTACAGA 
GAGCTGTTAC TAGAACACCA GGATGCGTAT CAGGCTGGAA TCGTGTTTCC TGATTGTTTT 
TACCCTAGCA TCTGCAAAGG AGGAAAATTC CATGATGTGT CTGAGAGCAC TCACTGGACT 
CCGTTTCTTA ATGCAAGCGT TCATTATATC CGAGAGAACT ATCCCCTTCC CTGGGAGAAG 
GACACAGAGA AACTGGTAGC TTTCTTGTTT GGAATTACTT CTCACATGGC GGCAGATGTC 
AGCTGGCATA GTCTGGGCCT TGAACAAGGA TTCCTTAGGA CCATGGGAGC TATTGATTTT 
CACGGCTCCT ATTCAGAGGC TCATTCGGCT GGTGATTTTG GAGGAGATGT GTTGAGCCAG 
TTTGAATTTA ATTTTAATTA CCTTGCACGA CGCTGGTATG TGCCAGTCAA AGATCTACTG 
GGAATTTATG AGAAACTGTA TGGTCGAAAA GTCATCACCG AAAATGTAAT CGTTGATTGT 
TCACATATCC AGTTCTTAGA AATGTATGGT GAGATGCTAG CTGTTTCCAA GTTATATCCC 
ACTTACTCTA CAAAGTCCCC GTTTTTGGTG GAACAATTCC AAGAGTATTT TCTTGGAGGA 
CTGGATGATA TGGCATTTTG GTCCACTAAT ATTTACCATC TAACAAGCTT CATGTTGGAG 
AATGGGACCA GTGACTGCAA CCTGCCTGAG AACCCTCTGT TCATTGCATG TGGCGGCCAG 
CAAAACCACA CCCAGGGCTC AAAAATGCAG AAAAATGATT TTCACAGAAA TTTGACTACA 
TCCCTAACTG AAAGTGTTGA CAGGAATATA AACTATACTG AAAGAGGAGT GTTCTTTAGT 
GTAAATTCCT GGACCCCGGA TTCCATGTCC TTTATCTACA AGGCTTTGGA AAGGAACATA 
AGGACAATGT TCATAGGTGG CTCTCAGTTG TCACAAAAGC ACGTCTCCAG CCCCTTAGCA 
TCTTACTTCT TGTCATTTCC TTATGCGAGG CTTGGCTGGG CAATGACCTC AGCTGACCTC 
AACCAGGATG GGCACGGTGA CCTCGTGGTG GGCGCACCAG GCTACAGCCG CCCCGGCCAC 
ATCCACATCG GGCGCGTGTA CCTCATCTAC GGCAATGACC TGGGCCTGCC ACCTGTTGAC 
CTGGACCTGG ACAAGGAGGC CCACAGGATC CTTGAAGGCT TCCAGCCCTC AGGTCGGTTT 
GGCTCGGCCT TGGCTGTGTT GGACTTTAAC GTGGACGGCG TGCCTGACCT GGCCGTGGGA 
GCTCCCTCGG TGGGCTCCGA GCAGCTCACC TACAAAGGTG CCGTGTATGT CTACTTTGGT 
TCCAAACAAG GAGGAATGTC TTCTTCCCCT AACATCACCA TTTCTTGCCA GATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512811.1
CDS	152..1708
Translation	MQVAMARATTATGNRLWPGLLIMLGSLCHRGSPCGLSTHVEIGHRALEFLQLHNGRVNYRELLLEHQDAYQAGIVFPDCFYPSICKGGKFHDVSESTHWTPFLNASVHYIRENYPLPWEKDTEKLVAFLFGITSHMAADVSWHSLGLEQGFLRTMGAIDFHGSYSEAHSAGDFGGDVLSQFEFNFNYLARRWYVPVKDLLGIYEKLYGRKVITENVIVDCSHIQFLEMYGEMLAVSKLYPTYSTKSPFLVEQFQEYFLGGLDDMAFWSTNIYHLTSFMLENGTSDCNLPENPLFIACGGQQNHTQGSKMQKNDFHRNLTTSLTESVDRNINYTERGVFFSVNSWTPDSMSFIYKALERNIRTMFIGGSQLSQKHVSSPLASYFLSFPYARLGWAMTSADLNQDGHGDLVVGAPGYSRPGHIHIGRVYLIYGNDLGLPPVDLDLDKEAHRILEGFQPSGRFGSALAVLDFNVDGVPDLAVGAPSVGSEQLTYKGAVYVYFGSKQGGMSSSPNITISCQM

Target ORF information:

RefSeq Version	XM_011514509.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514509.2

ORF Insert Sequence:

ATGCAGGTCG CCATGGCCCG GGCAACGACG GCGACAGGAA ACAGGTTGTG GCCTGGCCTG 
CTGATCATGT TGGGTTCTCT CTGCCATAGA GGTTCACCGT GTGGCCTTTC AACACACGTA 
GAAATAGGAC ACAGAGCTCT GGAGTTTCTT CAGCTTCACA ATGGGCGTGT TAACTACAGA 
GAGCTGTTAC TAGAACACCA GGATGCGTAT CAGGCTGGAA TCGTGTTTCC TGATTGTTTT 
TACCCTAGCA TCTGCAAAGG AGGAAAATTC CATGATGTGT CTGAGAGCAC TCACTGGACT 
CCGTTTCTTA ATGCAAGCGT TCATTATATC CGAGAGAACT ATCCCCTTCC CTGGGAGAAG 
GACACAGAGA AACTGGTAGC TTTCTTGTTT GGAATTACTT CTCACATGGC GGCAGATGTC 
AGCTGGCATA GTCTGGGCCT TGAACAAGGA TTCCTTAGGA CCATGGGAGC TATTGATTTT 
CACGGCTCCT ATTCAGAGGC TCATTCGGCT GGTGATTTTG GAGGAGATGT GTTGAGCCAG 
TTTGAATTTA ATTTTAATTA CCTTGCACGA CGCTGGTATG TGCCAGTCAA AGATCTACTG 
GGAATTTATG AGAAACTGTA TGGTCGAAAA GTCATCACCG AAAATGTAAT CGTTGATTGT 
TCACATATCC AGTTCTTAGA AATGTATGGT GAGATGCTAG CTGTTTCCAA GTTATATCCC 
ACTTACTCTA CAAAGTCCCC GTTTTTGGTG GAACAATTCC AAGAGTATTT TCTTGGAGGA 
CTGGATGATA TGGCATTTTG GTCCACTAAT ATTTACCATC TAACAAGCTT CATGTTGGAG 
AATGGGACCA GTGACTGCAA CCTGCCTGAG AACCCTCTGT TCATTGCATG TGGCGGCCAG 
CAAAACCACA CCCAGGGCTC AAAAATGCAG AAAAATGATT TTCACAGAAA TTTGACTACA 
TCCCTAACTG AAAGTGTTGA CAGGAATATA AACTATACTG AAAGAGGAGT GTTCTTTAGT 
GTAAATTCCT GGACCCCGGA TTCCATGTCC TTTATCTACA AGGCTTTGGA AAGGAACATA 
AGGACAATGT TCATAGGTGG CTCTCAGTTG TCACAAAAGC ACGTCTCCAG CCCCTTAGCA 
TCTTACTTCT TGTCATTTCC TTATGCGAGG CTTGGCTGGG CAATGACCTC AGCTGACCTC 
AACCAGGATG GGCACGGTGA CCTCGTGGTG GGCGCACCAG GCTACAGCCG CCCCGGCCAC 
ATCCACATCG GGCGCGTGTA CCTCATCTAC GGCAATGACC TGGGCCTGCC ACCTGTTGAC 
CTGGACCTGG ACAAGGAGGC CCACAGGATC CTTGAAGGCT TCCAGCCCTC AGGTCGGTTT 
GGCTCGGCCT TGGCTGTGTT GGACTTTAAC GTGGACGGCG TGCCTGACCT GGCCGTGGGA 
GCTCCCTCGG TGGGCTCCGA GCAGCTCACC TACAAAGGTG CCGTGTATGT CTACTTTGGT 
TCCAAACAAG GAGGAATGTC TTCTTCCCCT AACATCACCA TTTCTTGCCA GATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPLD1 ( XM_017010754.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866243.1 Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu40267
Clone ID Related Accession (Same CDS sequence)	XM_017010754.1 , XM_024446403.1
Accession Version	XM_017010754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan-specific phospholipase D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCTG GCATAGTCTG GGCCTTGAAC AAGGATTCCT TAGGACCATG GGAGCTGTAT 
GTGCCAGTCA AAGATCTACT GGGAATTTAT GAGAAACTGT ATGGTCGAAA AGTCATCACC 
GAAAATGTAA TCGTTGATTG TTCACATATC CAGTTCTTAG AAATGTATGG TGAGATGCTA 
GCTGTTTCCA AGTTATATCC CACTTACTCT ACAAAGTCCC CGTTTTTGGT GGAACAATTC 
CAAGAGTATT TTCTTGGAGG ACTGGATGAT ATGGCATTTT GGTCCACTAA TATTTACCAT 
CTAACAAGCT TCATGTTGGA GAATGGGACC AGTGACTGCA ACCTGCCTGA GAACCCTCTG 
TTCATTGCAT GTGGCGGCCA GCAAAACCAC ACCCAGGGCT CAAAAATGCA GAAAAATGAT 
TTTCACAGAA ATTTGACTAC ATCCCTAACT GAAAGTGTTG ACAGGAATAT AAACTATACT 
GAAAGAGGAG TGTTCTTTAG TGTAAATTCC TGGACCCCGG ATTCCATGTC CTTTATCTAC 
AAGGCTTTGG AAAGGAACAT AAGGACAATG TTCATAGGTG GCTCTCAGTT GTCACAAAAG 
CACGTCTCCA GCCCCTTAGC ATCTTACTTC TTGTCATTTC CTTATGCGAG GCTTGGCTGG 
GCAATGACCT CAGCTGACCT CAACCAGGAT GGGCACGGTG ACCTCGTGGT GGGCGCACCA 
GGCTACAGCC GCCCCGGCCA CATCCACATC GGGCGCGTGT ACCTCATCTA CGGCAATGAC 
CTGGGCCTGC CACCTGTTGA CCTGGACCTG GACAAGGAGG CCCACAGGAT CCTTGAAGGC 
TTCCAGCCCT CAGGTCGGTT TGGCTCGGCC TTGGCTGTGT TGGACTTTAA CGTGGACGGC 
GTGCCTGACC TGGCCGTGGG AGCTCCCTCG GTGGGCTCCG AGCAGCTCAC CTACAAAGGT 
GCCGTGTATG TCTACTTTGG TTCCAAACAA GGAGGAATGT CTTCTTCCCC TAACATCACC 
ATTTCTTGCC AGGACATCTA CTGTAACTTG GGCTGGACTC TCTTGGCTGC AGATGTGAAT 
GGAGACAGTG AACCCGATCT GGTCATCGGC TCCCCTTTTG CACCAGGTGG AGGGAAGCAG 
AAGGGAATTG TGGCTGCGTT TTATTCTGGC CCCAGCCTGA GCGACAAAGA AAAACTGAAC 
GTGGAGGCAG CCAACTGGAC GGTGAGAGGC GAGGAAGACT TCTCCTGGTT TGGATATTCC 
CTTCACGGTG TCACTGTGGA CAACAGAACC TTGCTGTTGG TTGGGAGCCC GACCTGGAAG 
AATGCCAGCA GGCTGGGCCA TTTGTTACAC ATCCGAGATG AGAAAAAGAG CCTTGGGAGG 
GTGTATGGCT ACTTCCCACC AAACGGCCAA AGCTGGTTTA CCATTTCTGG AGACAAGGCA 
ATGGGGAAAC TGGGTACTTC CCTTTCCAGT GGCCACGTAC TGATGAATGG GACTCTGAAA 
CAAGTGCTGC TGGTTGGAGC CCCTACGTAC GATGACGTGT CTAAGGTGGC ATTCCTGACC 
GTGACCCTAC ACCAAGGCGG AGCCACTCGC ATGTACGCAC TCACATCTGA CGCGCAGCCT 
CTGCTGCTCA GCACCTTCAG CGGAGACCGC CGCTTCTCCC GATTTGGTGG CGTTCTGCAC 
TTGAGTGACC TGGATGATGA TGGCTTAGAT GAAATCATCA TGGCAGCCCC CCTGAGGATA 
GCAGATGTAA CCTCTGGACT GATTGGGGGA GAAGACGGCC GAGTATATGT ATATAATGGC 
AAAGAGACCA CCCTTGGTGA CATGACTGGC AAATGCAAAT CATGGATAAC TCCATGTCCA 
GAAGAAAAGG CCCAATATGT ATTGATTTCT CCTGAAGCCA GCTCAAGGTT TGGGAGCTCC 
CTCATCACCG TGAGGTCCAA GGCAAAGAAC CAAGTCGTCA TTGCTGCTGG AAGGAGTTCT 
TTGGGAGCCC GACTCTCCGG GGCACTTCAC GTCTATAGCC TTGGCTCAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866243.1
CDS	92..2125
Translation	MSAGIVWALNKDSLGPWELYVPVKDLLGIYEKLYGRKVITENVIVDCSHIQFLEMYGEMLAVSKLYPTYSTKSPFLVEQFQEYFLGGLDDMAFWSTNIYHLTSFMLENGTSDCNLPENPLFIACGGQQNHTQGSKMQKNDFHRNLTTSLTESVDRNINYTERGVFFSVNSWTPDSMSFIYKALERNIRTMFIGGSQLSQKHVSSPLASYFLSFPYARLGWAMTSADLNQDGHGDLVVGAPGYSRPGHIHIGRVYLIYGNDLGLPPVDLDLDKEAHRILEGFQPSGRFGSALAVLDFNVDGVPDLAVGAPSVGSEQLTYKGAVYVYFGSKQGGMSSSPNITISCQDIYCNLGWTLLAADVNGDSEPDLVIGSPFAPGGGKQKGIVAAFYSGPSLSDKEKLNVEAANWTVRGEEDFSWFGYSLHGVTVDNRTLLLVGSPTWKNASRLGHLLHIRDEKKSLGRVYGYFPPNGQSWFTISGDKAMGKLGTSLSSGHVLMNGTLKQVLLVGAPTYDDVSKVAFLTVTLHQGGATRMYALTSDAQPLLLSTFSGDRRFSRFGGVLHLSDLDDDGLDEIIMAAPLRIADVTSGLIGGEDGRVYVYNGKETTLGDMTGKCKSWITPCPEEKAQYVLISPEASSRFGSSLITVRSKAKNQVVIAAGRSSLGARLSGALHVYSLGSD

Target ORF information:

RefSeq Version	XM_017010754.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010754.1

ORF Insert Sequence:

ATGTCAGCTG GCATAGTCTG GGCCTTGAAC AAGGATTCCT TAGGACCATG GGAGCTGTAT 
GTGCCAGTCA AAGATCTACT GGGAATTTAT GAGAAACTGT ATGGTCGAAA AGTCATCACC 
GAAAATGTAA TCGTTGATTG TTCACATATC CAGTTCTTAG AAATGTATGG TGAGATGCTA 
GCTGTTTCCA AGTTATATCC CACTTACTCT ACAAAGTCCC CGTTTTTGGT GGAACAATTC 
CAAGAGTATT TTCTTGGAGG ACTGGATGAT ATGGCATTTT GGTCCACTAA TATTTACCAT 
CTAACAAGCT TCATGTTGGA GAATGGGACC AGTGACTGCA ACCTGCCTGA GAACCCTCTG 
TTCATTGCAT GTGGCGGCCA GCAAAACCAC ACCCAGGGCT CAAAAATGCA GAAAAATGAT 
TTTCACAGAA ATTTGACTAC ATCCCTAACT GAAAGTGTTG ACAGGAATAT AAACTATACT 
GAAAGAGGAG TGTTCTTTAG TGTAAATTCC TGGACCCCGG ATTCCATGTC CTTTATCTAC 
AAGGCTTTGG AAAGGAACAT AAGGACAATG TTCATAGGTG GCTCTCAGTT GTCACAAAAG 
CACGTCTCCA GCCCCTTAGC ATCTTACTTC TTGTCATTTC CTTATGCGAG GCTTGGCTGG 
GCAATGACCT CAGCTGACCT CAACCAGGAT GGGCACGGTG ACCTCGTGGT GGGCGCACCA 
GGCTACAGCC GCCCCGGCCA CATCCACATC GGGCGCGTGT ACCTCATCTA CGGCAATGAC 
CTGGGCCTGC CACCTGTTGA CCTGGACCTG GACAAGGAGG CCCACAGGAT CCTTGAAGGC 
TTCCAGCCCT CAGGTCGGTT TGGCTCGGCC TTGGCTGTGT TGGACTTTAA CGTGGACGGC 
GTGCCTGACC TGGCCGTGGG AGCTCCCTCG GTGGGCTCCG AGCAGCTCAC CTACAAAGGT 
GCCGTGTATG TCTACTTTGG TTCCAAACAA GGAGGAATGT CTTCTTCCCC TAACATCACC 
ATTTCTTGCC AGGACATCTA CTGTAACTTG GGCTGGACTC TCTTGGCTGC AGATGTGAAT 
GGAGACAGTG AACCCGATCT GGTCATCGGC TCCCCTTTTG CACCAGGTGG AGGGAAGCAG 
AAGGGAATTG TGGCTGCGTT TTATTCTGGC CCCAGCCTGA GCGACAAAGA AAAACTGAAC 
GTGGAGGCAG CCAACTGGAC GGTGAGAGGC GAGGAAGACT TCTCCTGGTT TGGATATTCC 
CTTCACGGTG TCACTGTGGA CAACAGAACC TTGCTGTTGG TTGGGAGCCC GACCTGGAAG 
AATGCCAGCA GGCTGGGCCA TTTGTTACAC ATCCGAGATG AGAAAAAGAG CCTTGGGAGG 
GTGTATGGCT ACTTCCCACC AAACGGCCAA AGCTGGTTTA CCATTTCTGG AGACAAGGCA 
ATGGGGAAAC TGGGTACTTC CCTTTCCAGT GGCCACGTAC TGATGAATGG GACTCTGAAA 
CAAGTGCTGC TGGTTGGAGC CCCTACGTAC GATGACGTGT CTAAGGTGGC ATTCCTGACC 
GTGACCCTAC ACCAAGGCGG AGCCACTCGC ATGTACGCAC TCACATCTGA CGCGCAGCCT 
CTGCTGCTCA GCACCTTCAG CGGAGACCGC CGCTTCTCCC GATTTGGTGG CGTTCTGCAC 
TTGAGTGACC TGGATGATGA TGGCTTAGAT GAAATCATCA TGGCAGCCCC CCTGAGGATA 
GCAGATGTAA CCTCTGGACT GATTGGGGGA GAAGACGGCC GAGTATATGT ATATAATGGC 
AAAGAGACCA CCCTTGGTGA CATGACTGGC AAATGCAAAT CATGGATAAC TCCATGTCCA 
GAAGAAAAGG CCCAATATGT ATTGATTTCT CCTGAAGCCA GCTCAAGGTT TGGGAGCTCC 
CTCATCACCG TGAGGTCCAA GGCAAAGAAC CAAGTCGTCA TTGCTGCTGG AAGGAGTTCT 
TTGGGAGCCC GACTCTCCGG GGCACTTCAC GTCTATAGCC TTGGCTCAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPLD1 ( XM_024446403.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302171.1 Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu40267
Clone ID Related Accession (Same CDS sequence)	XM_017010754.1 , XM_024446403.1
Accession Version	XM_024446403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan-specific phospholipase D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCTG GCATAGTCTG GGCCTTGAAC AAGGATTCCT TAGGACCATG GGAGCTGTAT 
GTGCCAGTCA AAGATCTACT GGGAATTTAT GAGAAACTGT ATGGTCGAAA AGTCATCACC 
GAAAATGTAA TCGTTGATTG TTCACATATC CAGTTCTTAG AAATGTATGG TGAGATGCTA 
GCTGTTTCCA AGTTATATCC CACTTACTCT ACAAAGTCCC CGTTTTTGGT GGAACAATTC 
CAAGAGTATT TTCTTGGAGG ACTGGATGAT ATGGCATTTT GGTCCACTAA TATTTACCAT 
CTAACAAGCT TCATGTTGGA GAATGGGACC AGTGACTGCA ACCTGCCTGA GAACCCTCTG 
TTCATTGCAT GTGGCGGCCA GCAAAACCAC ACCCAGGGCT CAAAAATGCA GAAAAATGAT 
TTTCACAGAA ATTTGACTAC ATCCCTAACT GAAAGTGTTG ACAGGAATAT AAACTATACT 
GAAAGAGGAG TGTTCTTTAG TGTAAATTCC TGGACCCCGG ATTCCATGTC CTTTATCTAC 
AAGGCTTTGG AAAGGAACAT AAGGACAATG TTCATAGGTG GCTCTCAGTT GTCACAAAAG 
CACGTCTCCA GCCCCTTAGC ATCTTACTTC TTGTCATTTC CTTATGCGAG GCTTGGCTGG 
GCAATGACCT CAGCTGACCT CAACCAGGAT GGGCACGGTG ACCTCGTGGT GGGCGCACCA 
GGCTACAGCC GCCCCGGCCA CATCCACATC GGGCGCGTGT ACCTCATCTA CGGCAATGAC 
CTGGGCCTGC CACCTGTTGA CCTGGACCTG GACAAGGAGG CCCACAGGAT CCTTGAAGGC 
TTCCAGCCCT CAGGTCGGTT TGGCTCGGCC TTGGCTGTGT TGGACTTTAA CGTGGACGGC 
GTGCCTGACC TGGCCGTGGG AGCTCCCTCG GTGGGCTCCG AGCAGCTCAC CTACAAAGGT 
GCCGTGTATG TCTACTTTGG TTCCAAACAA GGAGGAATGT CTTCTTCCCC TAACATCACC 
ATTTCTTGCC AGGACATCTA CTGTAACTTG GGCTGGACTC TCTTGGCTGC AGATGTGAAT 
GGAGACAGTG AACCCGATCT GGTCATCGGC TCCCCTTTTG CACCAGGTGG AGGGAAGCAG 
AAGGGAATTG TGGCTGCGTT TTATTCTGGC CCCAGCCTGA GCGACAAAGA AAAACTGAAC 
GTGGAGGCAG CCAACTGGAC GGTGAGAGGC GAGGAAGACT TCTCCTGGTT TGGATATTCC 
CTTCACGGTG TCACTGTGGA CAACAGAACC TTGCTGTTGG TTGGGAGCCC GACCTGGAAG 
AATGCCAGCA GGCTGGGCCA TTTGTTACAC ATCCGAGATG AGAAAAAGAG CCTTGGGAGG 
GTGTATGGCT ACTTCCCACC AAACGGCCAA AGCTGGTTTA CCATTTCTGG AGACAAGGCA 
ATGGGGAAAC TGGGTACTTC CCTTTCCAGT GGCCACGTAC TGATGAATGG GACTCTGAAA 
CAAGTGCTGC TGGTTGGAGC CCCTACGTAC GATGACGTGT CTAAGGTGGC ATTCCTGACC 
GTGACCCTAC ACCAAGGCGG AGCCACTCGC ATGTACGCAC TCACATCTGA CGCGCAGCCT 
CTGCTGCTCA GCACCTTCAG CGGAGACCGC CGCTTCTCCC GATTTGGTGG CGTTCTGCAC 
TTGAGTGACC TGGATGATGA TGGCTTAGAT GAAATCATCA TGGCAGCCCC CCTGAGGATA 
GCAGATGTAA CCTCTGGACT GATTGGGGGA GAAGACGGCC GAGTATATGT ATATAATGGC 
AAAGAGACCA CCCTTGGTGA CATGACTGGC AAATGCAAAT CATGGATAAC TCCATGTCCA 
GAAGAAAAGG CCCAATATGT ATTGATTTCT CCTGAAGCCA GCTCAAGGTT TGGGAGCTCC 
CTCATCACCG TGAGGTCCAA GGCAAAGAAC CAAGTCGTCA TTGCTGCTGG AAGGAGTTCT 
TTGGGAGCCC GACTCTCCGG GGCACTTCAC GTCTATAGCC TTGGCTCAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302171.1
CDS	446..2479
Translation	MSAGIVWALNKDSLGPWELYVPVKDLLGIYEKLYGRKVITENVIVDCSHIQFLEMYGEMLAVSKLYPTYSTKSPFLVEQFQEYFLGGLDDMAFWSTNIYHLTSFMLENGTSDCNLPENPLFIACGGQQNHTQGSKMQKNDFHRNLTTSLTESVDRNINYTERGVFFSVNSWTPDSMSFIYKALERNIRTMFIGGSQLSQKHVSSPLASYFLSFPYARLGWAMTSADLNQDGHGDLVVGAPGYSRPGHIHIGRVYLIYGNDLGLPPVDLDLDKEAHRILEGFQPSGRFGSALAVLDFNVDGVPDLAVGAPSVGSEQLTYKGAVYVYFGSKQGGMSSSPNITISCQDIYCNLGWTLLAADVNGDSEPDLVIGSPFAPGGGKQKGIVAAFYSGPSLSDKEKLNVEAANWTVRGEEDFSWFGYSLHGVTVDNRTLLLVGSPTWKNASRLGHLLHIRDEKKSLGRVYGYFPPNGQSWFTISGDKAMGKLGTSLSSGHVLMNGTLKQVLLVGAPTYDDVSKVAFLTVTLHQGGATRMYALTSDAQPLLLSTFSGDRRFSRFGGVLHLSDLDDDGLDEIIMAAPLRIADVTSGLIGGEDGRVYVYNGKETTLGDMTGKCKSWITPCPEEKAQYVLISPEASSRFGSSLITVRSKAKNQVVIAAGRSSLGARLSGALHVYSLGSD

Target ORF information:

RefSeq Version	XM_024446403.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446403.1

ORF Insert Sequence:

ATGTCAGCTG GCATAGTCTG GGCCTTGAAC AAGGATTCCT TAGGACCATG GGAGCTGTAT 
GTGCCAGTCA AAGATCTACT GGGAATTTAT GAGAAACTGT ATGGTCGAAA AGTCATCACC 
GAAAATGTAA TCGTTGATTG TTCACATATC CAGTTCTTAG AAATGTATGG TGAGATGCTA 
GCTGTTTCCA AGTTATATCC CACTTACTCT ACAAAGTCCC CGTTTTTGGT GGAACAATTC 
CAAGAGTATT TTCTTGGAGG ACTGGATGAT ATGGCATTTT GGTCCACTAA TATTTACCAT 
CTAACAAGCT TCATGTTGGA GAATGGGACC AGTGACTGCA ACCTGCCTGA GAACCCTCTG 
TTCATTGCAT GTGGCGGCCA GCAAAACCAC ACCCAGGGCT CAAAAATGCA GAAAAATGAT 
TTTCACAGAA ATTTGACTAC ATCCCTAACT GAAAGTGTTG ACAGGAATAT AAACTATACT 
GAAAGAGGAG TGTTCTTTAG TGTAAATTCC TGGACCCCGG ATTCCATGTC CTTTATCTAC 
AAGGCTTTGG AAAGGAACAT AAGGACAATG TTCATAGGTG GCTCTCAGTT GTCACAAAAG 
CACGTCTCCA GCCCCTTAGC ATCTTACTTC TTGTCATTTC CTTATGCGAG GCTTGGCTGG 
GCAATGACCT CAGCTGACCT CAACCAGGAT GGGCACGGTG ACCTCGTGGT GGGCGCACCA 
GGCTACAGCC GCCCCGGCCA CATCCACATC GGGCGCGTGT ACCTCATCTA CGGCAATGAC 
CTGGGCCTGC CACCTGTTGA CCTGGACCTG GACAAGGAGG CCCACAGGAT CCTTGAAGGC 
TTCCAGCCCT CAGGTCGGTT TGGCTCGGCC TTGGCTGTGT TGGACTTTAA CGTGGACGGC 
GTGCCTGACC TGGCCGTGGG AGCTCCCTCG GTGGGCTCCG AGCAGCTCAC CTACAAAGGT 
GCCGTGTATG TCTACTTTGG TTCCAAACAA GGAGGAATGT CTTCTTCCCC TAACATCACC 
ATTTCTTGCC AGGACATCTA CTGTAACTTG GGCTGGACTC TCTTGGCTGC AGATGTGAAT 
GGAGACAGTG AACCCGATCT GGTCATCGGC TCCCCTTTTG CACCAGGTGG AGGGAAGCAG 
AAGGGAATTG TGGCTGCGTT TTATTCTGGC CCCAGCCTGA GCGACAAAGA AAAACTGAAC 
GTGGAGGCAG CCAACTGGAC GGTGAGAGGC GAGGAAGACT TCTCCTGGTT TGGATATTCC 
CTTCACGGTG TCACTGTGGA CAACAGAACC TTGCTGTTGG TTGGGAGCCC GACCTGGAAG 
AATGCCAGCA GGCTGGGCCA TTTGTTACAC ATCCGAGATG AGAAAAAGAG CCTTGGGAGG 
GTGTATGGCT ACTTCCCACC AAACGGCCAA AGCTGGTTTA CCATTTCTGG AGACAAGGCA 
ATGGGGAAAC TGGGTACTTC CCTTTCCAGT GGCCACGTAC TGATGAATGG GACTCTGAAA 
CAAGTGCTGC TGGTTGGAGC CCCTACGTAC GATGACGTGT CTAAGGTGGC ATTCCTGACC 
GTGACCCTAC ACCAAGGCGG AGCCACTCGC ATGTACGCAC TCACATCTGA CGCGCAGCCT 
CTGCTGCTCA GCACCTTCAG CGGAGACCGC CGCTTCTCCC GATTTGGTGG CGTTCTGCAC 
TTGAGTGACC TGGATGATGA TGGCTTAGAT GAAATCATCA TGGCAGCCCC CCTGAGGATA 
GCAGATGTAA CCTCTGGACT GATTGGGGGA GAAGACGGCC GAGTATATGT ATATAATGGC 
AAAGAGACCA CCCTTGGTGA CATGACTGGC AAATGCAAAT CATGGATAAC TCCATGTCCA 
GAAGAAAAGG CCCAATATGT ATTGATTTCT CCTGAAGCCA GCTCAAGGTT TGGGAGCTCC 
CTCATCACCG TGAGGTCCAA GGCAAAGAAC CAAGTCGTCA TTGCTGCTGG AAGGAGTTCT 
TTGGGAGCCC GACTCTCCGG GGCACTTCAC GTCTATAGCC TTGGCTCAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPLD1 ( XM_017010753.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866242.1 Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu96547
Clone ID Related Accession (Same CDS sequence)	XM_017010753.1 , XM_017010753.2
Accession Version	XM_017010753.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan-specific phospholipase D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTCG CCATGGCCCG GGCAACGACG GCGACAGGAA ACAGGTTGTG GCCTGGCCTG 
CTGATCATGT TGGGTTCTCT CTGCCATAGA GGTTCACCGT GTGGCCTTTC AACACACGTA 
GAAATAGGAC ACAGAGCTCT GGAGTTTCTT CAGCTTCACA ATGGGCGTGT TAACTACAGA 
GAGCTGTTAC TAGAACACCA GGATGCGTAT CAGGCTGGAA TCGTGTTTCC TGATTGTTTT 
TACCCTAGCA TCTGCAAAGG AGGAAAATTC CATGATGTGT CTGAGAGCAC TCACTGGACT 
CCGTTTCTTA ATGCAAGCGT TCATTATATC CGAGAGAACT ATCCCCTTCC CTGGGAGAAG 
GACACAGAGA AACTGGTAGC TTTCTTGTTT GGAATTACTT CTCACATGGC GGCAGATGTC 
AGCTGGCATA GTCTGGGCCT TGAACAAGGA TTCCTTAGGA CCATGGGAGC TATTGATTTT 
CACGGCTCCT ATTCAGAGGC TCATTCGGCT GGTGATTTTG GAGGAGATGT GTTGAGCCAG 
TTTGAATTTA ATTTTAATTA CCTTGCACGA CGCTGGTATG TGCCAGTCAA AGATCTACTG 
GGAATTTATG AGAAACTGTA TGGTCGAAAA GTCATCACCG AAAATGTAAT CGTTGATTGT 
TCACATATCC AGTTCTTAGA AATGTATGGT GAGATGCTAG CTGTTTCCAA GTTATATCCC 
ACTTACTCTA CAAAGTCCCC GTTTTTGGTG GAACAATTCC AAGAGTATTT TCTTGGAGGA 
CTGGATGATA TGGCATTTTG GTCCACTAAT ATTTACCATC TAACAAGCTT CATGTTGGAG 
AATGGGACCA GTGACTGCAA CCTGCCTGAG AACCCTCTGT TCATTGCATG TGGCGGCCAG 
CAAAACCACA CCCAGGGCTC AAAAATGCAG AAAAATGATT TTCACAGAAA TTTGACTACA 
TCCCTAACTG AAAGTGTTGA CAGGAATATA AACTATACTG AAAGAGGAGT GTTCTTTAGT 
GTAAATTCCT GGACCCCGGA TTCCATGTCC TTTATCTACA AGGCTTTGGA AAGGAACATA 
AGGACAATGT TCATAGGTGG CTCTCAGTTG TCACAAAAGC ACGTCTCCAG CCCCTTAGCA 
TCTTACTTCT TGTCATTTCC TTATGCGAGG CTTGGCTGGG CAATGACCTC AGCTGACCTC 
AACCAGGATG GGCACGGTGA CCTCGTGGTG GGCGCACCAG GCTACAGCCG CCCCGGCCAC 
ATCCACATCG GGCGCGTGTA CCTCATCTAC GGCAATGACC TGGGCCTGCC ACCTGTTGAC 
CTGGACCTGG ACAAGGAGGC CCACAGGATC CTTGAAGGCT TCCAGCCCTC AGGTCGGTTT 
GGCTCGGCCT TGGCTGTGTT GGACTTTAAC GTGGACGGCG TGCCTGACCT GGCCGTGGGA 
GCTCCCTCGG TGGGCTCCGA GCAGCTCACC TACAAAGGTG CCGTGTATGT CTACTTTGGT 
TCCAAACAAG GAGGAATGTC TTCTTCCCCT AACATCACCA TTTCTTGCCA GGACATCTAC 
TGTAACTTGG GCTGGACTCT CTTGGCTGCA GATGTGAATG GAGACAGTGA ACCCGATCTG 
GTCATCGGCT CCCCTTTTGC ACCAGGTGGA GGGAAGCAGA AGGGAATTGT GGCTGCGTTT 
TATTCTGGCC CCAGCCTGAG CGACAAAGAA AAACTGAACG TGGAGGCAGC CAACTGGACG 
GTGAGAGGCG AGGAAGACTT CTCCTGGTTT GGATATTCCC TTCACGGTGT CACTGTGGAC 
AACAGAACCT TGCTGTTGGT TGGGAGCCCG ACCTGGAAGA ATGCCAGCAG GCTGGGCCAT 
TTGTTACACA TCCGAGATGA GAAAAAGAGC CTTGGGAGGG TGTATGGCTA CTTCCCACCA 
AACGGCCAAA GCTGGTTTAC CATTTCTGGA GACAAGGCAA TGGGGAAACT GGGTACTTCC 
CTTTCCAGTG GCCACGTACT GATGAATGGG ACTCTGAAAC AAGTGCTGCT GGTTGGAGCC 
CCTACGTACG ATGACGTGTC TAAGGTGGCA TTCCTGACCG TGACCCTACA CCAAGGCGGA 
GCCACTCGCA TGTACGCACT CACATCTGAC GCGCAGCCTC TGCTGCTCAG CACCTTCAGC 
GGAGACCGCC GCTTCTCCCG ATTTGGTGGC GTTCTGCACT TGAGTGACCT GGATGATGAT 
GGCTTAGATG AAATCATCAT GGCAGCCCCC CTGAGGATAG CAGATGTAAC CTCTGGACTG 
ATTGGGGGAG AAGACGGCCG AGTATATGTA TATAATGGCA AAGAGACCAC CCTTGGTGAC 
ATGACTGGCA AATGCAAATC ATGGATAACT CCATGTCCAG AAGAAAAGGC CCAATATGTA 
TTGATTTCTC CTGAAGCCAG CTCAAGGTTT GGGAGCTCCC TCATCACCGT GAGGTCCAAG 
GCAAAGAACC AAGTCGTCAT TGCTGCTGGA AGGAGTTCTT TGGGAGCCCG ACTCTCCGGG 
GCACTTCACG TCTATAGCCT TGGCTCAGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866242.1
CDS	154..2706
Translation	MQVAMARATTATGNRLWPGLLIMLGSLCHRGSPCGLSTHVEIGHRALEFLQLHNGRVNYRELLLEHQDAYQAGIVFPDCFYPSICKGGKFHDVSESTHWTPFLNASVHYIRENYPLPWEKDTEKLVAFLFGITSHMAADVSWHSLGLEQGFLRTMGAIDFHGSYSEAHSAGDFGGDVLSQFEFNFNYLARRWYVPVKDLLGIYEKLYGRKVITENVIVDCSHIQFLEMYGEMLAVSKLYPTYSTKSPFLVEQFQEYFLGGLDDMAFWSTNIYHLTSFMLENGTSDCNLPENPLFIACGGQQNHTQGSKMQKNDFHRNLTTSLTESVDRNINYTERGVFFSVNSWTPDSMSFIYKALERNIRTMFIGGSQLSQKHVSSPLASYFLSFPYARLGWAMTSADLNQDGHGDLVVGAPGYSRPGHIHIGRVYLIYGNDLGLPPVDLDLDKEAHRILEGFQPSGRFGSALAVLDFNVDGVPDLAVGAPSVGSEQLTYKGAVYVYFGSKQGGMSSSPNITISCQDIYCNLGWTLLAADVNGDSEPDLVIGSPFAPGGGKQKGIVAAFYSGPSLSDKEKLNVEAANWTVRGEEDFSWFGYSLHGVTVDNRTLLLVGSPTWKNASRLGHLLHIRDEKKSLGRVYGYFPPNGQSWFTISGDKAMGKLGTSLSSGHVLMNGTLKQVLLVGAPTYDDVSKVAFLTVTLHQGGATRMYALTSDAQPLLLSTFSGDRRFSRFGGVLHLSDLDDDGLDEIIMAAPLRIADVTSGLIGGEDGRVYVYNGKETTLGDMTGKCKSWITPCPEEKAQYVLISPEASSRFGSSLITVRSKAKNQVVIAAGRSSLGARLSGALHVYSLGSD

Target ORF information:

RefSeq Version	XM_017010753.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010753.1

ORF Insert Sequence:

ATGCAGGTCG CCATGGCCCG GGCAACGACG GCGACAGGAA ACAGGTTGTG GCCTGGCCTG 
CTGATCATGT TGGGTTCTCT CTGCCATAGA GGTTCACCGT GTGGCCTTTC AACACACGTA 
GAAATAGGAC ACAGAGCTCT GGAGTTTCTT CAGCTTCACA ATGGGCGTGT TAACTACAGA 
GAGCTGTTAC TAGAACACCA GGATGCGTAT CAGGCTGGAA TCGTGTTTCC TGATTGTTTT 
TACCCTAGCA TCTGCAAAGG AGGAAAATTC CATGATGTGT CTGAGAGCAC TCACTGGACT 
CCGTTTCTTA ATGCAAGCGT TCATTATATC CGAGAGAACT ATCCCCTTCC CTGGGAGAAG 
GACACAGAGA AACTGGTAGC TTTCTTGTTT GGAATTACTT CTCACATGGC GGCAGATGTC 
AGCTGGCATA GTCTGGGCCT TGAACAAGGA TTCCTTAGGA CCATGGGAGC TATTGATTTT 
CACGGCTCCT ATTCAGAGGC TCATTCGGCT GGTGATTTTG GAGGAGATGT GTTGAGCCAG 
TTTGAATTTA ATTTTAATTA CCTTGCACGA CGCTGGTATG TGCCAGTCAA AGATCTACTG 
GGAATTTATG AGAAACTGTA TGGTCGAAAA GTCATCACCG AAAATGTAAT CGTTGATTGT 
TCACATATCC AGTTCTTAGA AATGTATGGT GAGATGCTAG CTGTTTCCAA GTTATATCCC 
ACTTACTCTA CAAAGTCCCC GTTTTTGGTG GAACAATTCC AAGAGTATTT TCTTGGAGGA 
CTGGATGATA TGGCATTTTG GTCCACTAAT ATTTACCATC TAACAAGCTT CATGTTGGAG 
AATGGGACCA GTGACTGCAA CCTGCCTGAG AACCCTCTGT TCATTGCATG TGGCGGCCAG 
CAAAACCACA CCCAGGGCTC AAAAATGCAG AAAAATGATT TTCACAGAAA TTTGACTACA 
TCCCTAACTG AAAGTGTTGA CAGGAATATA AACTATACTG AAAGAGGAGT GTTCTTTAGT 
GTAAATTCCT GGACCCCGGA TTCCATGTCC TTTATCTACA AGGCTTTGGA AAGGAACATA 
AGGACAATGT TCATAGGTGG CTCTCAGTTG TCACAAAAGC ACGTCTCCAG CCCCTTAGCA 
TCTTACTTCT TGTCATTTCC TTATGCGAGG CTTGGCTGGG CAATGACCTC AGCTGACCTC 
AACCAGGATG GGCACGGTGA CCTCGTGGTG GGCGCACCAG GCTACAGCCG CCCCGGCCAC 
ATCCACATCG GGCGCGTGTA CCTCATCTAC GGCAATGACC TGGGCCTGCC ACCTGTTGAC 
CTGGACCTGG ACAAGGAGGC CCACAGGATC CTTGAAGGCT TCCAGCCCTC AGGTCGGTTT 
GGCTCGGCCT TGGCTGTGTT GGACTTTAAC GTGGACGGCG TGCCTGACCT GGCCGTGGGA 
GCTCCCTCGG TGGGCTCCGA GCAGCTCACC TACAAAGGTG CCGTGTATGT CTACTTTGGT 
TCCAAACAAG GAGGAATGTC TTCTTCCCCT AACATCACCA TTTCTTGCCA GGACATCTAC 
TGTAACTTGG GCTGGACTCT CTTGGCTGCA GATGTGAATG GAGACAGTGA ACCCGATCTG 
GTCATCGGCT CCCCTTTTGC ACCAGGTGGA GGGAAGCAGA AGGGAATTGT GGCTGCGTTT 
TATTCTGGCC CCAGCCTGAG CGACAAAGAA AAACTGAACG TGGAGGCAGC CAACTGGACG 
GTGAGAGGCG AGGAAGACTT CTCCTGGTTT GGATATTCCC TTCACGGTGT CACTGTGGAC 
AACAGAACCT TGCTGTTGGT TGGGAGCCCG ACCTGGAAGA ATGCCAGCAG GCTGGGCCAT 
TTGTTACACA TCCGAGATGA GAAAAAGAGC CTTGGGAGGG TGTATGGCTA CTTCCCACCA 
AACGGCCAAA GCTGGTTTAC CATTTCTGGA GACAAGGCAA TGGGGAAACT GGGTACTTCC 
CTTTCCAGTG GCCACGTACT GATGAATGGG ACTCTGAAAC AAGTGCTGCT GGTTGGAGCC 
CCTACGTACG ATGACGTGTC TAAGGTGGCA TTCCTGACCG TGACCCTACA CCAAGGCGGA 
GCCACTCGCA TGTACGCACT CACATCTGAC GCGCAGCCTC TGCTGCTCAG CACCTTCAGC 
GGAGACCGCC GCTTCTCCCG ATTTGGTGGC GTTCTGCACT TGAGTGACCT GGATGATGAT 
GGCTTAGATG AAATCATCAT GGCAGCCCCC CTGAGGATAG CAGATGTAAC CTCTGGACTG 
ATTGGGGGAG AAGACGGCCG AGTATATGTA TATAATGGCA AAGAGACCAC CCTTGGTGAC 
ATGACTGGCA AATGCAAATC ATGGATAACT CCATGTCCAG AAGAAAAGGC CCAATATGTA 
TTGATTTCTC CTGAAGCCAG CTCAAGGTTT GGGAGCTCCC TCATCACCGT GAGGTCCAAG 
GCAAAGAACC AAGTCGTCAT TGCTGCTGGA AGGAGTTCTT TGGGAGCCCG ACTCTCCGGG 
GCACTTCACG TCTATAGCCT TGGCTCAGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPLD1 ( XM_017010753.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866242.1 Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu96547
Clone ID Related Accession (Same CDS sequence)	XM_017010753.1 , XM_017010753.2
Accession Version	XM_017010753.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	phosphatidylinositol-glycan-specific phospholipase D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017010753.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTCG CCATGGCCCG GGCAACGACG GCGACAGGAA ACAGGTTGTG GCCTGGCCTG 
CTGATCATGT TGGGTTCTCT CTGCCATAGA GGTTCACCGT GTGGCCTTTC AACACACGTA 
GAAATAGGAC ACAGAGCTCT GGAGTTTCTT CAGCTTCACA ATGGGCGTGT TAACTACAGA 
GAGCTGTTAC TAGAACACCA GGATGCGTAT CAGGCTGGAA TCGTGTTTCC TGATTGTTTT 
TACCCTAGCA TCTGCAAAGG AGGAAAATTC CATGATGTGT CTGAGAGCAC TCACTGGACT 
CCGTTTCTTA ATGCAAGCGT TCATTATATC CGAGAGAACT ATCCCCTTCC CTGGGAGAAG 
GACACAGAGA AACTGGTAGC TTTCTTGTTT GGAATTACTT CTCACATGGC GGCAGATGTC 
AGCTGGCATA GTCTGGGCCT TGAACAAGGA TTCCTTAGGA CCATGGGAGC TATTGATTTT 
CACGGCTCCT ATTCAGAGGC TCATTCGGCT GGTGATTTTG GAGGAGATGT GTTGAGCCAG 
TTTGAATTTA ATTTTAATTA CCTTGCACGA CGCTGGTATG TGCCAGTCAA AGATCTACTG 
GGAATTTATG AGAAACTGTA TGGTCGAAAA GTCATCACCG AAAATGTAAT CGTTGATTGT 
TCACATATCC AGTTCTTAGA AATGTATGGT GAGATGCTAG CTGTTTCCAA GTTATATCCC 
ACTTACTCTA CAAAGTCCCC GTTTTTGGTG GAACAATTCC AAGAGTATTT TCTTGGAGGA 
CTGGATGATA TGGCATTTTG GTCCACTAAT ATTTACCATC TAACAAGCTT CATGTTGGAG 
AATGGGACCA GTGACTGCAA CCTGCCTGAG AACCCTCTGT TCATTGCATG TGGCGGCCAG 
CAAAACCACA CCCAGGGCTC AAAAATGCAG AAAAATGATT TTCACAGAAA TTTGACTACA 
TCCCTAACTG AAAGTGTTGA CAGGAATATA AACTATACTG AAAGAGGAGT GTTCTTTAGT 
GTAAATTCCT GGACCCCGGA TTCCATGTCC TTTATCTACA AGGCTTTGGA AAGGAACATA 
AGGACAATGT TCATAGGTGG CTCTCAGTTG TCACAAAAGC ACGTCTCCAG CCCCTTAGCA 
TCTTACTTCT TGTCATTTCC TTATGCGAGG CTTGGCTGGG CAATGACCTC AGCTGACCTC 
AACCAGGATG GGCACGGTGA CCTCGTGGTG GGCGCACCAG GCTACAGCCG CCCCGGCCAC 
ATCCACATCG GGCGCGTGTA CCTCATCTAC GGCAATGACC TGGGCCTGCC ACCTGTTGAC 
CTGGACCTGG ACAAGGAGGC CCACAGGATC CTTGAAGGCT TCCAGCCCTC AGGTCGGTTT 
GGCTCGGCCT TGGCTGTGTT GGACTTTAAC GTGGACGGCG TGCCTGACCT GGCCGTGGGA 
GCTCCCTCGG TGGGCTCCGA GCAGCTCACC TACAAAGGTG CCGTGTATGT CTACTTTGGT 
TCCAAACAAG GAGGAATGTC TTCTTCCCCT AACATCACCA TTTCTTGCCA GGACATCTAC 
TGTAACTTGG GCTGGACTCT CTTGGCTGCA GATGTGAATG GAGACAGTGA ACCCGATCTG 
GTCATCGGCT CCCCTTTTGC ACCAGGTGGA GGGAAGCAGA AGGGAATTGT GGCTGCGTTT 
TATTCTGGCC CCAGCCTGAG CGACAAAGAA AAACTGAACG TGGAGGCAGC CAACTGGACG 
GTGAGAGGCG AGGAAGACTT CTCCTGGTTT GGATATTCCC TTCACGGTGT CACTGTGGAC 
AACAGAACCT TGCTGTTGGT TGGGAGCCCG ACCTGGAAGA ATGCCAGCAG GCTGGGCCAT 
TTGTTACACA TCCGAGATGA GAAAAAGAGC CTTGGGAGGG TGTATGGCTA CTTCCCACCA 
AACGGCCAAA GCTGGTTTAC CATTTCTGGA GACAAGGCAA TGGGGAAACT GGGTACTTCC 
CTTTCCAGTG GCCACGTACT GATGAATGGG ACTCTGAAAC AAGTGCTGCT GGTTGGAGCC 
CCTACGTACG ATGACGTGTC TAAGGTGGCA TTCCTGACCG TGACCCTACA CCAAGGCGGA 
GCCACTCGCA TGTACGCACT CACATCTGAC GCGCAGCCTC TGCTGCTCAG CACCTTCAGC 
GGAGACCGCC GCTTCTCCCG ATTTGGTGGC GTTCTGCACT TGAGTGACCT GGATGATGAT 
GGCTTAGATG AAATCATCAT GGCAGCCCCC CTGAGGATAG CAGATGTAAC CTCTGGACTG 
ATTGGGGGAG AAGACGGCCG AGTATATGTA TATAATGGCA AAGAGACCAC CCTTGGTGAC 
ATGACTGGCA AATGCAAATC ATGGATAACT CCATGTCCAG AAGAAAAGGC CCAATATGTA 
TTGATTTCTC CTGAAGCCAG CTCAAGGTTT GGGAGCTCCC TCATCACCGT GAGGTCCAAG 
GCAAAGAACC AAGTCGTCAT TGCTGCTGGA AGGAGTTCTT TGGGAGCCCG ACTCTCCGGG 
GCACTTCACG TCTATAGCCT TGGCTCAGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866242.1
CDS	223..2775
Translation	MQVAMARATTATGNRLWPGLLIMLGSLCHRGSPCGLSTHVEIGHRALEFLQLHNGRVNYRELLLEHQDAYQAGIVFPDCFYPSICKGGKFHDVSESTHWTPFLNASVHYIRENYPLPWEKDTEKLVAFLFGITSHMAADVSWHSLGLEQGFLRTMGAIDFHGSYSEAHSAGDFGGDVLSQFEFNFNYLARRWYVPVKDLLGIYEKLYGRKVITENVIVDCSHIQFLEMYGEMLAVSKLYPTYSTKSPFLVEQFQEYFLGGLDDMAFWSTNIYHLTSFMLENGTSDCNLPENPLFIACGGQQNHTQGSKMQKNDFHRNLTTSLTESVDRNINYTERGVFFSVNSWTPDSMSFIYKALERNIRTMFIGGSQLSQKHVSSPLASYFLSFPYARLGWAMTSADLNQDGHGDLVVGAPGYSRPGHIHIGRVYLIYGNDLGLPPVDLDLDKEAHRILEGFQPSGRFGSALAVLDFNVDGVPDLAVGAPSVGSEQLTYKGAVYVYFGSKQGGMSSSPNITISCQDIYCNLGWTLLAADVNGDSEPDLVIGSPFAPGGGKQKGIVAAFYSGPSLSDKEKLNVEAANWTVRGEEDFSWFGYSLHGVTVDNRTLLLVGSPTWKNASRLGHLLHIRDEKKSLGRVYGYFPPNGQSWFTISGDKAMGKLGTSLSSGHVLMNGTLKQVLLVGAPTYDDVSKVAFLTVTLHQGGATRMYALTSDAQPLLLSTFSGDRRFSRFGGVLHLSDLDDDGLDEIIMAAPLRIADVTSGLIGGEDGRVYVYNGKETTLGDMTGKCKSWITPCPEEKAQYVLISPEASSRFGSSLITVRSKAKNQVVIAAGRSSLGARLSGALHVYSLGSD

Target ORF information:

RefSeq Version	XM_017010753.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010753.2

ORF Insert Sequence:

ATGCAGGTCG CCATGGCCCG GGCAACGACG GCGACAGGAA ACAGGTTGTG GCCTGGCCTG 
CTGATCATGT TGGGTTCTCT CTGCCATAGA GGTTCACCGT GTGGCCTTTC AACACACGTA 
GAAATAGGAC ACAGAGCTCT GGAGTTTCTT CAGCTTCACA ATGGGCGTGT TAACTACAGA 
GAGCTGTTAC TAGAACACCA GGATGCGTAT CAGGCTGGAA TCGTGTTTCC TGATTGTTTT 
TACCCTAGCA TCTGCAAAGG AGGAAAATTC CATGATGTGT CTGAGAGCAC TCACTGGACT 
CCGTTTCTTA ATGCAAGCGT TCATTATATC CGAGAGAACT ATCCCCTTCC CTGGGAGAAG 
GACACAGAGA AACTGGTAGC TTTCTTGTTT GGAATTACTT CTCACATGGC GGCAGATGTC 
AGCTGGCATA GTCTGGGCCT TGAACAAGGA TTCCTTAGGA CCATGGGAGC TATTGATTTT 
CACGGCTCCT ATTCAGAGGC TCATTCGGCT GGTGATTTTG GAGGAGATGT GTTGAGCCAG 
TTTGAATTTA ATTTTAATTA CCTTGCACGA CGCTGGTATG TGCCAGTCAA AGATCTACTG 
GGAATTTATG AGAAACTGTA TGGTCGAAAA GTCATCACCG AAAATGTAAT CGTTGATTGT 
TCACATATCC AGTTCTTAGA AATGTATGGT GAGATGCTAG CTGTTTCCAA GTTATATCCC 
ACTTACTCTA CAAAGTCCCC GTTTTTGGTG GAACAATTCC AAGAGTATTT TCTTGGAGGA 
CTGGATGATA TGGCATTTTG GTCCACTAAT ATTTACCATC TAACAAGCTT CATGTTGGAG 
AATGGGACCA GTGACTGCAA CCTGCCTGAG AACCCTCTGT TCATTGCATG TGGCGGCCAG 
CAAAACCACA CCCAGGGCTC AAAAATGCAG AAAAATGATT TTCACAGAAA TTTGACTACA 
TCCCTAACTG AAAGTGTTGA CAGGAATATA AACTATACTG AAAGAGGAGT GTTCTTTAGT 
GTAAATTCCT GGACCCCGGA TTCCATGTCC TTTATCTACA AGGCTTTGGA AAGGAACATA 
AGGACAATGT TCATAGGTGG CTCTCAGTTG TCACAAAAGC ACGTCTCCAG CCCCTTAGCA 
TCTTACTTCT TGTCATTTCC TTATGCGAGG CTTGGCTGGG CAATGACCTC AGCTGACCTC 
AACCAGGATG GGCACGGTGA CCTCGTGGTG GGCGCACCAG GCTACAGCCG CCCCGGCCAC 
ATCCACATCG GGCGCGTGTA CCTCATCTAC GGCAATGACC TGGGCCTGCC ACCTGTTGAC 
CTGGACCTGG ACAAGGAGGC CCACAGGATC CTTGAAGGCT TCCAGCCCTC AGGTCGGTTT 
GGCTCGGCCT TGGCTGTGTT GGACTTTAAC GTGGACGGCG TGCCTGACCT GGCCGTGGGA 
GCTCCCTCGG TGGGCTCCGA GCAGCTCACC TACAAAGGTG CCGTGTATGT CTACTTTGGT 
TCCAAACAAG GAGGAATGTC TTCTTCCCCT AACATCACCA TTTCTTGCCA GGACATCTAC 
TGTAACTTGG GCTGGACTCT CTTGGCTGCA GATGTGAATG GAGACAGTGA ACCCGATCTG 
GTCATCGGCT CCCCTTTTGC ACCAGGTGGA GGGAAGCAGA AGGGAATTGT GGCTGCGTTT 
TATTCTGGCC CCAGCCTGAG CGACAAAGAA AAACTGAACG TGGAGGCAGC CAACTGGACG 
GTGAGAGGCG AGGAAGACTT CTCCTGGTTT GGATATTCCC TTCACGGTGT CACTGTGGAC 
AACAGAACCT TGCTGTTGGT TGGGAGCCCG ACCTGGAAGA ATGCCAGCAG GCTGGGCCAT 
TTGTTACACA TCCGAGATGA GAAAAAGAGC CTTGGGAGGG TGTATGGCTA CTTCCCACCA 
AACGGCCAAA GCTGGTTTAC CATTTCTGGA GACAAGGCAA TGGGGAAACT GGGTACTTCC 
CTTTCCAGTG GCCACGTACT GATGAATGGG ACTCTGAAAC AAGTGCTGCT GGTTGGAGCC 
CCTACGTACG ATGACGTGTC TAAGGTGGCA TTCCTGACCG TGACCCTACA CCAAGGCGGA 
GCCACTCGCA TGTACGCACT CACATCTGAC GCGCAGCCTC TGCTGCTCAG CACCTTCAGC 
GGAGACCGCC GCTTCTCCCG ATTTGGTGGC GTTCTGCACT TGAGTGACCT GGATGATGAT 
GGCTTAGATG AAATCATCAT GGCAGCCCCC CTGAGGATAG CAGATGTAAC CTCTGGACTG 
ATTGGGGGAG AAGACGGCCG AGTATATGTA TATAATGGCA AAGAGACCAC CCTTGGTGAC 
ATGACTGGCA AATGCAAATC ATGGATAACT CCATGTCCAG AAGAAAAGGC CCAATATGTA 
TTGATTTCTC CTGAAGCCAG CTCAAGGTTT GGGAGCTCCC TCATCACCGT GAGGTCCAAG 
GCAAAGAACC AAGTCGTCAT TGCTGCTGGA AGGAGTTCTT TGGGAGCCCG ACTCTCCGGG 
GCACTTCACG TCTATAGCCT TGGCTCAGAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of curcumin in PLD activation by Arf6-cytohesin1 signaling axis in U46619-stimulated pulmonary artery smooth muscle cells.
Molecular and cellular biochemistry438(1-2)97-109(2018 Jan)
Chakraborti S,Sarkar J,Bhuyan R,Chakraborti T

Regulation of glycosylphosphatidylinositol-anchored proteins and GPI-phospholipase D in a c-Myc transgenic mouse model of hepatocellular carcinoma and human HCC.
Biological chemistry397(11)1147-1162(2016 Nov)
Ritorto MS,Rhode H,Vogel A,Borlak J

MASP1, THBS1, GPLD1 and ApoA-IV are novel biomarkers associated with prediabetes: the KORA F4 study.
Diabetologia59(9)1882-92(2016 Sep)
von Toerne C,Huth C,de Las Heras Gala T,Kronenberg F,Herder C,Koenig W,Meisinger C,Rathmann W,Waldenberger M,Roden M,Peters A,Thorand B,Hauck SM

Down-regulation of MicroRNAs (MiRs) 203, 887, 3619 and 182 Prevents Vimentin-triggered, Phospholipase D (PLD)-mediated Cancer Cell Invasion.
The Journal of biological chemistry291(2)719-30(2016 Jan)
Fite K,Gomez-Cambronero J

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GPLD1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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