GCHFR cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCHFR
Entrez Gene ID	2644
Full Name	GTP cyclohydrolase I feedback regulator
Synonyms	GFRP,HsT16933,P35
General protein information	Preferred Names GTP cyclohydrolase I feedback regulator Names GTP cyclohydrolase 1 feedback regulatory protein GTP cyclohydrolase I feedback regulatory protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q15.1
Summary	GTP cyclohydrolase I feedback regulatory protein binds to and mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase I. The regulatory protein, GCHFR, consists of a homodimer. It is postulated that GCHFR may play a role in regulating phenylalanine metabolism in the liver and in the production of biogenic amine neurotransmitters and nitric oxide. [provided by RefSeq, Jul 2008].
Disorder MIM:	602437

mRNA and Protein(s)

mRNA	Protein	Name
NM_005258.2	NP_005249.1	GTP cyclohydrolase 1 feedback regulatory protein
NM_005258.3	NP_005249.1	GTP cyclohydrolase 1 feedback regulatory protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	GCHFR	NP_005249.1
Canis lupus familiaris (dog)	GCHFR	XP_544622.2
Mus musculus (house mouse)	Gchfr	NP_796131.1
Gallus gallus (chicken)	GCHFR	NP_001186451.1
Pan troglodytes (chimpanzee)	GCHFR	XP_001146591.1
Xenopus tropicalis (tropical clawed frog)	gchfr	NP_998880.1
	GCHFR	XP_001097382.2
Bos taurus (cattle)	GCHFR	NP_001107998.1
Rattus norvegicus (Norway rat)	Gchfr	NP_598279.1
Danio rerio (zebrafish)	gchfr	NP_957056.1
Caenorhabditis elegans (roundworm)	gfrp-1	NP_001041021.2

Gene Ontology
Bibliography

Related articles in PubMed

A polymorphism of the GTP-cyclohydrolase I feedback regulator gene alters transcriptional activity and may affect response to SSRI antidepressants.
McHugh PC, Joyce PR, Deng X, Kennedy MA
The pharmacogenomics journal11(3)207-13(2011 Jun)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

GTP cyclohydrolase I phosphorylation and interaction with GTP cyclohydrolase feedback regulatory protein provide novel regulation of endothelial tetrahydrobiopterin and nitric oxide.
Li L, Rezvan A, Salerno JC, Husain A, Kwon K, Jo H, Harrison DG, Chen W
Circulation research106(2)328-36(2010 Feb)

Examination of tetrahydrobiopterin pathway genes in autism.
Schnetz-Boutaud NC, Anderson BM, Brown KD, Wright HH, Abramson RK, Cuccaro ML, Gilbert JR, Pericak-Vance MA, Haines JL
Genes, brain, and behavior8(8)753-7(2009 Nov)

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
Talmud PJ, Drenos F, Shah S, Shah T, Palmen J, Verzilli C, Gaunt TR, Pallas J, Lovering R, Li K, Casas JP, Sofat R, Kumari M, Rodriguez S, Johnson T, Newhouse SJ, Dominiczak A, Samani NJ, Caulfield M, Sever P, Stanton A, Shields DC, Padmanabhan S, Melander O, Hastie C, Delles C, Ebrahim S, Marmot MG, Smith GD, Lawlor DA, Munroe PB, Day IN, Kivimaki M, Whittaker J, Humphries SE, Hingorani AD, , ,
American journal of human genetics85(5)628-42(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: A polymorphism of the GTP-cyclohydrolase I feedback regulator gene alters transcriptional activity and may affect response to SSRI antidepressants.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Studies provide a new mechanism for regulation of endothelial GTPCH-1 by its phosphorylation and interplay with GFRP.
Title: GTP cyclohydrolase I phosphorylation and interaction with GTP cyclohydrolase feedback regulatory protein provide novel regulation of endothelial tetrahydrobiopterin and nitric oxide.

Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
Title: Examination of tetrahydrobiopterin pathway genes in autism.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following GCHFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCHFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29455	NM_005258.3 Latest version!	Homo sapiens GTP cyclohydrolase I feedback regulator (GCHFR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu29455	NM_005258.2	Homo sapiens GTP cyclohydrolase I feedback regulator (GCHFR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCHFR ( NM_005258.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005249.1 Homo sapiens GTP cyclohydrolase I feedback regulator (GCHFR), mRNA.

Price & Availability↑

CloneID	OHu29455
Clone ID Related Accession (Same CDS sequence)	NM_005258.2 , NM_005258.3
Accession Version	NM_005258.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	GTP cyclohydrolase 1 feedback regulatory protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U78190.1. On Nov 12, 1999 this sequence version replaced NM_005258.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AA100037.1, SRR1163655.660530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTACC TGCTCATCAG CACCCAGATC CGCATGGAGG TGGGCCCCAC TATGGTGGGC 
GATGAACAGT CGGATCCAGA GCTGATGCAG CATCTGGGGG CTTCAAAGAG AAGAGCCTTG 
GGAAACAACT TTTATGAATA CTACGTCGAT GACCCTCCCC GCATAGTCCT GGACAAGCTG 
GAACGCAGGG GCTTCCGTGT GCTGAGCATG ACGGGGGTGG GCCAGACGCT GGTGTGGTGT 
CTGCACAAGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005249.1
CDS	95..349
Translation	MPYLLISTQIRMEVGPTMVGDEQSDPELMQHLGASKRRALGNNFYEYYVDDPPRIVLDKLERRGFRVLSMTGVGQTLVWCLHKE

Target ORF information:

RefSeq Version	NM_005258.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP cyclohydrolase I feedback regulator (GCHFR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005258.2

ORF Insert Sequence:

ATGCCCTACC TGCTCATCAG CACCCAGATC CGCATGGAGG TGGGCCCCAC TATGGTGGGC 
GATGAACAGT CGGATCCAGA GCTGATGCAG CATCTGGGGG CTTCAAAGAG AAGAGCCTTG 
GGAAACAACT TTTATGAATA CTACGTCGAT GACCCTCCCC GCATAGTCCT GGACAAGCTG 
GAACGCAGGG GCTTCCGTGT GCTGAGCATG ACGGGGGTGG GCCAGACGCT GGTGTGGTGT 
CTGCACAAGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCHFR ( NM_005258.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005249.1 Homo sapiens GTP cyclohydrolase I feedback regulator (GCHFR), mRNA.

Price & Availability↑

CloneID	OHu29455
Clone ID Related Accession (Same CDS sequence)	NM_005258.2 , NM_005258.3
Accession Version	NM_005258.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	GTP cyclohydrolase 1 feedback regulatory protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012476.8. On Nov 26, 2018 this sequence version replaced NM_005258.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223430.1, SRR1163657.441672.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000260447.6/ ENSP00000260447.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCTACC TGCTCATCAG CACCCAGATC CGCATGGAGG TGGGCCCCAC TATGGTGGGC 
GATGAACAGT CGGATCCAGA GCTGATGCAG CATCTGGGGG CTTCAAAGAG AAGAGCCTTG 
GGAAACAACT TTTATGAATA CTACGTCGAT GACCCTCCCC GCATAGTCCT GGACAAGCTG 
GAACGCAGGG GCTTCCGTGT GCTGAGCATG ACGGGGGTGG GCCAGACGCT GGTGTGGTGT 
CTGCACAAGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005249.1
CDS	114..368
Translation	MPYLLISTQIRMEVGPTMVGDEQSDPELMQHLGASKRRALGNNFYEYYVDDPPRIVLDKLERRGFRVLSMTGVGQTLVWCLHKE

Target ORF information:

RefSeq Version	NM_005258.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP cyclohydrolase I feedback regulator (GCHFR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005258.3

ORF Insert Sequence:

ATGCCCTACC TGCTCATCAG CACCCAGATC CGCATGGAGG TGGGCCCCAC TATGGTGGGC 
GATGAACAGT CGGATCCAGA GCTGATGCAG CATCTGGGGG CTTCAAAGAG AAGAGCCTTG 
GGAAACAACT TTTATGAATA CTACGTCGAT GACCCTCCCC GCATAGTCCT GGACAAGCTG 
GAACGCAGGG GCTTCCGTGT GCTGAGCATG ACGGGGGTGG GCCAGACGCT GGTGTGGTGT 
CTGCACAAGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A polymorphism of the GTP-cyclohydrolase I feedback regulator gene alters transcriptional activity and may affect response to SSRI antidepressants.
The pharmacogenomics journal11(3)207-13(2011 Jun)
McHugh PC,Joyce PR,Deng X,Kennedy MA

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,

GTP cyclohydrolase I phosphorylation and interaction with GTP cyclohydrolase feedback regulatory protein provide novel regulation of endothelial tetrahydrobiopterin and nitric oxide.
Circulation research106(2)328-36(2010 Feb)
Li L,Rezvan A,Salerno JC,Husain A,Kwon K,Jo H,Harrison DG,Chen W

Examination of tetrahydrobiopterin pathway genes in autism.
Genes, brain, and behavior8(8)753-7(2009 Nov)
Schnetz-Boutaud NC,Anderson BM,Brown KD,Wright HH,Abramson RK,Cuccaro ML,Gilbert JR,Pericak-Vance MA,Haines JL

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
American journal of human genetics85(5)628-42(2009 Nov)
Talmud PJ,Drenos F,Shah S,Shah T,Palmen J,Verzilli C,Gaunt TR,Pallas J,Lovering R,Li K,Casas JP,Sofat R,Kumari M,Rodriguez S,Johnson T,Newhouse SJ,Dominiczak A,Samani NJ,Caulfield M,Sever P,Stanton A,Shields DC,Padmanabhan S,Melander O,Hastie C,Delles C,Ebrahim S,Marmot MG,Smith GD,Lawlor DA,Munroe PB,Day IN,Kivimaki M,Whittaker J,Humphries SE,Hingorani AD,,,

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GCHFR cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

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電話

FAX

オンラインフォーム