EDRF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EDRF1
Entrez Gene ID	26098
Full Name	erythroid differentiation regulatory factor 1
Synonyms	C10orf137
General protein information	Preferred Names erythroid differentiation regulatory factor 1 Names erythroid differentiation-related factor 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q26.2
Summary	This gene may play a role in erythroid cell differentiation. The encoded protein inhibits DNA binding of the erythroid transcription factor GATA-1 and may regulate the expression of alpha-globin and gamma-globin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_015608.2	NP_056423.2	erythroid differentiation-related factor 1 isoform 2
XM_017016066.1	XP_016871555.1	erythroid differentiation-related factor 1 isoform X1
NM_001202438.1	NP_001189367.1	erythroid differentiation-related factor 1 isoform 1
NM_001202438.2	NP_001189367.1	erythroid differentiation-related factor 1 isoform 1
XM_017016066.2	XP_016871555.1	erythroid differentiation-related factor 1 isoform X1
XM_011539622.1	XP_011537924.1	erythroid differentiation-related factor 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	EDRF1	NP_001189367.1
Drosophila melanogaster (fruit fly)	CG6511	NP_648252.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001324	XP_321822.5
	LOC695685	XP_002805895.1
Canis lupus familiaris (dog)	C28H10orf137	XP_003640049.1
Danio rerio (zebrafish)	LOC566404	XP_694764.4
Xenopus tropicalis (tropical clawed frog)	LOC100497935	XP_004912778.1
Pan troglodytes (chimpanzee)	C10H10orf137	XP_001137992.1
Mus musculus (house mouse)	Edrf1	NP_835216.3
Rattus norvegicus (Norway rat)	RGD1306820	NP_001101027.1
Gallus gallus (chicken)	C6H10ORF137	XP_428227.4
Bos taurus (cattle)	C26H10orf137	XP_005225922.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel erythroid differentiation related gene EDRF1 upregulating globin gene expression in HEL cells.
Wang D, Li Y, Shen B
Chinese medical journal115(11)1701-5(2002 Nov)

Antisense EDRF1 gene inhibited GATA-1 transcription factor DNA-binding activity in K562 cells.
Wang D, Ma S, Wang S, Shen B
Science in China. Series C, Life sciences45(3)289-97(2002 Jun)

Initial function analysis of a novel erythroid differentiation related gene EDRF1.
Wang D, Li Y, Shen B
Science in China. Series C, Life sciences44(5)489-96(2001 Oct)

cDNA cloning and function analysis of two novel erythroid differentiation related genes.
Wang X, Wang D, Chen X, Hu M, Wang J, Li Y, Guo N, Shen B
Science in China. Series C, Life sciences44(1)99-105(2001 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

EDRF1 regulated alpha- and gamma-globin gene synthesis by modulating DNA-binding activity of GATA-1 transcription factor.
Title: A novel erythroid differentiation related gene EDRF1 upregulating globin gene expression in HEL cells.

The following EDRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu66815	XM_017016066.2 Latest version!	Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu66815	XM_011539622.1 Latest version!	Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu08826	NM_015608.2 Latest version!	Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu09248	NM_001202438.2 Latest version!	Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu09248	NM_001202438.1	Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu66815	XM_017016066.1	Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EDRF1 ( XM_017016066.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871555.1 Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu66815
Clone ID Related Accession (Same CDS sequence)	XM_017016066.1 , XM_017016066.2 , XM_011539622.1
Accession Version	XM_017016066.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	erythroid differentiation-related factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGT ACAGCTACCT CACATGCTGT TATTCCTATT TGATGGAAAG AGAGACTGAG 
GATCAAGGAG CTCAAAAATA TGAAATGATA AAGACAGAAG AAATTCCCAA TTTGGAAAAT 
TCTAATTTTT CTACTAAAGT CATAAAAGAC ATTGCACAGA ATATTTTATC ATTTTTGAAA 
TCTAATTGTA CCAAAGAAGG ACATACCTAT TGGCTCTTTA AAGCAAGTGG CAGCGATATA 
GTGAAGCTCT ATGACCTCAC TACTCTTTGT GAAGAAACTG AAGACAAATA CCAAAATCCA 
TTCACAATGC CGGTAGCCAT TCTCTTGTAC AAGGTTGCTT GCAACATGAT GATGAAGAAG 
AATCAAAATA AGAAACACTA TGGAACTATT AGAACATTGC TTCTTAATTG TCTGAAATTA 
CTGGACAAAA GTAGGCATCC TCAAATTATT GCTTCCGCCA ATTACATGCT TTCAGAACTT 
TTTCAATTGG ATGAACCTAA AAAGGAAGAA AATTCAGAAT CTCCTTTAAA TGAGAATTCT 
GATGAAAGTT ATAGTGAAGA GGAGGAAGAG ATGCCCGACA GTGATGAAAA TGGATCCTAT 
AGCACCAGCT CTGATCCATC AGATGATAGC AAAGCAGTAG CTATAATCAA GTCTGTTGGA 
GAACTATCAG TACCAGAAAA ATACAAATCT ATTCATCAAA TCAGACCCAG TTGTGCATTT 
CCAGTTTGCC ATGACACAGA AGAGCGCTGT AGACTTGTGC TTAGCTATGT TCTAGAGGGT 
TTAAAATCTG TCGATAGCAG CATCAAAAAA GAAAGCGACC TTCCAGCAGC TGACCCCAGC 
ACTCCAATCC CGTTAAAATA TGAAGATGAA TCCTCAAGAG GGGGTCCCGA GGGGCTAGAG 
AAGCAGATGG CCTTGTTTTT GGACAAAATG GGCTCCCTTC AGAAGGGCAA TTATTCCAGT 
CAATCTGGAA TGATCCCTGG CTCTTGGCAA CATAAAATGA AACTTCAGCT GATTCTCAAG 
TCATCAAAGG CCTATTATGT TTTGTCCGAT GCTGCCATGA GTCTTCAGAA ATACGGAAGA 
GCATTACGAT ACATTAAATT AGCTTTGCAA AGCCATGATA CTTATTGCTG CCTCTGCACC 
AATATGCTTT CCGAAGTGCT GTTGTTTCTC TCTCAATATT TGACACTTTG TGGTGATATC 
CAACTAATGC TGGCCCAGAA TGCAAATAAT AGAGCAGCAC ACCTTGAAGA GTTTCATTAC 
CAAACAAAAG AAGACCAGGA GATCCTGCAT AGCCTTCACA GAGAGTCCAG TTGCCAAGGA 
TTTGCATGGG CAACTGATTT GTCTACAGAC TTAGAAAGTC AACTCTCTGT TAGTTGTAAA 
TGTTATGAGG CTGCTAATGA AATCTTGCAG TTTAGTGACT TGAAAAGCCA AAATCCAGAA 
CACTATGTAC AAGTATTAAA GAGAATGGGT AACATTAGAA ATGAAATTGG TGTGTTTTAC 
ATGAATCAGG CTGCTGCATT ACAGAGTGAG AGACTAGTGA GCAAATCTGT GTCTGCTGCC 
GAGCAACAGT TGTGGAAAAA AAGCTTTTCT TGTTTTGAAA AGGGAATTCA CAACTTTGAA 
TCAATTGAGG ATGCCACCAA TGCCGCCCTT TTATTATGTA ACACGGGAAG GCTCATGCGG 
ATTTGTGCGC AGGCCCACTG TGGTGCAGGG GATGAACTGA AACGTGAATT TTCACCAGAA 
GAAGGCTTGT ATTATAATAA GGCTATTGAT TACTATTTGA AAGCGCTAAG GTCATTGGGA 
ACACGAGACA TACACCCAGC TGTTTGGGAT TCAGTGAACT GGGAATTGTC CACTACTTAC 
TTTACTATGG CAACTCTACA GCAAGATTAT GCTCCGTTAT CTAGAAAAGC TCAGGAGCAG 
ATTGAGAAAG AAGTCAGTGA GGCCATGATG AAGTCCCTAA AATACTGCGA TGTGGATTCA 
GTGTCTGCTC GACAGCCCCT TTGTCAGTAT CGAGCTGCAA CCATCCATCA CAGGCTGGCC 
TCCATGTACC ACAGCTGTCT GAGGAATCAG GTTGGTGATG AACACCTTAG GAAACAACAC 
CGGGTGCTGG CAGATCTTCA TTACAGCAAG GCCGCAAAGC TGTTTCAGCT GCTGAAAGAT 
GCTCCCTGCG AACTGCTTAG AGTACAGCTA GAGAGAGTAG CATTTGCTGA ATTTCAGATG 
ACCAGTCAGA ATAGCAATGT TGGAAAGTTG AAAACACTAT CTGGGGCTCT TGATATAATG 
GTGAGAACTG AGCACGCATT CCAGCTTATC CAGAAGGAGC TTATAGAAGA ATTTGGCCAG 
CCTAAGAGTG GTGACGCCGC TGCAGCTGCT GATGCTTCTC CTAGTCTCAA TCGAGAAGAA 
GTGATGAAAC TCCTCAGTAT ATTTGAGTCT CGGTTGTCAT TTCTTCTCCT TCAGTCCATT 
AAACTGCTAT CTTCAACTAA AAAGAAAACA AGCAATAACA TCGAAGATGA CACAATTCTC 
AAAACCAACA AGCACATTTA CTCCCAGCTT TTGAGAGCAA CTGCAAATAA AACCGCGACT 
CTTCTGGAAA GAATCAACGT TATCGTCCAC CTGCTGGGCC AGCTTGCCGC CGGCAGTGCA 
GCGAGCAGCA ATGCCGTTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871555.1
CDS	1091..3754
Translation	MELYSYLTCCYSYLMERETEDQGAQKYEMIKTEEIPNLENSNFSTKVIKDIAQNILSFLKSNCTKEGHTYWLFKASGSDIVKLYDLTTLCEETEDKYQNPFTMPVAILLYKVACNMMMKKNQNKKHYGTIRTLLLNCLKLLDKSRHPQIIASANYMLSELFQLDEPKKEENSESPLNENSDESYSEEEEEMPDSDENGSYSTSSDPSDDSKAVAIIKSVGELSVPEKYKSIHQIRPSCAFPVCHDTEERCRLVLSYVLEGLKSVDSSIKKESDLPAADPSTPIPLKYEDESSRGGPEGLEKQMALFLDKMGSLQKGNYSSQSGMIPGSWQHKMKLQLILKSSKAYYVLSDAAMSLQKYGRALRYIKLALQSHDTYCCLCTNMLSEVLLFLSQYLTLCGDIQLMLAQNANNRAAHLEEFHYQTKEDQEILHSLHRESSCQGFAWATDLSTDLESQLSVSCKCYEAANEILQFSDLKSQNPEHYVQVLKRMGNIRNEIGVFYMNQAAALQSERLVSKSVSAAEQQLWKKSFSCFEKGIHNFESIEDATNAALLLCNTGRLMRICAQAHCGAGDELKREFSPEEGLYYNKAIDYYLKALRSLGTRDIHPAVWDSVNWELSTTYFTMATLQQDYAPLSRKAQEQIEKEVSEAMMKSLKYCDVDSVSARQPLCQYRAATIHHRLASMYHSCLRNQVGDEHLRKQHRVLADLHYSKAAKLFQLLKDAPCELLRVQLERVAFAEFQMTSQNSNVGKLKTLSGALDIMVRTEHAFQLIQKELIEEFGQPKSGDAAAAADASPSLNREEVMKLLSIFESRLSFLLLQSIKLLSSTKKKTSNNIEDDTILKTNKHIYSQLLRATANKTATLLERINVIVHLLGQLAAGSAASSNAVQ

Target ORF information:

RefSeq Version	XM_017016066.1
Organism	Homo sapiens(human)
Definition	Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016066.1

ORF Insert Sequence:

ATGGAATTGT ACAGCTACCT CACATGCTGT TATTCCTATT TGATGGAAAG AGAGACTGAG 
GATCAAGGAG CTCAAAAATA TGAAATGATA AAGACAGAAG AAATTCCCAA TTTGGAAAAT 
TCTAATTTTT CTACTAAAGT CATAAAAGAC ATTGCACAGA ATATTTTATC ATTTTTGAAA 
TCTAATTGTA CCAAAGAAGG ACATACCTAT TGGCTCTTTA AAGCAAGTGG CAGCGATATA 
GTGAAGCTCT ATGACCTCAC TACTCTTTGT GAAGAAACTG AAGACAAATA CCAAAATCCA 
TTCACAATGC CGGTAGCCAT TCTCTTGTAC AAGGTTGCTT GCAACATGAT GATGAAGAAG 
AATCAAAATA AGAAACACTA TGGAACTATT AGAACATTGC TTCTTAATTG TCTGAAATTA 
CTGGACAAAA GTAGGCATCC TCAAATTATT GCTTCCGCCA ATTACATGCT TTCAGAACTT 
TTTCAATTGG ATGAACCTAA AAAGGAAGAA AATTCAGAAT CTCCTTTAAA TGAGAATTCT 
GATGAAAGTT ATAGTGAAGA GGAGGAAGAG ATGCCCGACA GTGATGAAAA TGGATCCTAT 
AGCACCAGCT CTGATCCATC AGATGATAGC AAAGCAGTAG CTATAATCAA GTCTGTTGGA 
GAACTATCAG TACCAGAAAA ATACAAATCT ATTCATCAAA TCAGACCCAG TTGTGCATTT 
CCAGTTTGCC ATGACACAGA AGAGCGCTGT AGACTTGTGC TTAGCTATGT TCTAGAGGGT 
TTAAAATCTG TCGATAGCAG CATCAAAAAA GAAAGCGACC TTCCAGCAGC TGACCCCAGC 
ACTCCAATCC CGTTAAAATA TGAAGATGAA TCCTCAAGAG GGGGTCCCGA GGGGCTAGAG 
AAGCAGATGG CCTTGTTTTT GGACAAAATG GGCTCCCTTC AGAAGGGCAA TTATTCCAGT 
CAATCTGGAA TGATCCCTGG CTCTTGGCAA CATAAAATGA AACTTCAGCT GATTCTCAAG 
TCATCAAAGG CCTATTATGT TTTGTCCGAT GCTGCCATGA GTCTTCAGAA ATACGGAAGA 
GCATTACGAT ACATTAAATT AGCTTTGCAA AGCCATGATA CTTATTGCTG CCTCTGCACC 
AATATGCTTT CCGAAGTGCT GTTGTTTCTC TCTCAATATT TGACACTTTG TGGTGATATC 
CAACTAATGC TGGCCCAGAA TGCAAATAAT AGAGCAGCAC ACCTTGAAGA GTTTCATTAC 
CAAACAAAAG AAGACCAGGA GATCCTGCAT AGCCTTCACA GAGAGTCCAG TTGCCAAGGA 
TTTGCATGGG CAACTGATTT GTCTACAGAC TTAGAAAGTC AACTCTCTGT TAGTTGTAAA 
TGTTATGAGG CTGCTAATGA AATCTTGCAG TTTAGTGACT TGAAAAGCCA AAATCCAGAA 
CACTATGTAC AAGTATTAAA GAGAATGGGT AACATTAGAA ATGAAATTGG TGTGTTTTAC 
ATGAATCAGG CTGCTGCATT ACAGAGTGAG AGACTAGTGA GCAAATCTGT GTCTGCTGCC 
GAGCAACAGT TGTGGAAAAA AAGCTTTTCT TGTTTTGAAA AGGGAATTCA CAACTTTGAA 
TCAATTGAGG ATGCCACCAA TGCCGCCCTT TTATTATGTA ACACGGGAAG GCTCATGCGG 
ATTTGTGCGC AGGCCCACTG TGGTGCAGGG GATGAACTGA AACGTGAATT TTCACCAGAA 
GAAGGCTTGT ATTATAATAA GGCTATTGAT TACTATTTGA AAGCGCTAAG GTCATTGGGA 
ACACGAGACA TACACCCAGC TGTTTGGGAT TCAGTGAACT GGGAATTGTC CACTACTTAC 
TTTACTATGG CAACTCTACA GCAAGATTAT GCTCCGTTAT CTAGAAAAGC TCAGGAGCAG 
ATTGAGAAAG AAGTCAGTGA GGCCATGATG AAGTCCCTAA AATACTGCGA TGTGGATTCA 
GTGTCTGCTC GACAGCCCCT TTGTCAGTAT CGAGCTGCAA CCATCCATCA CAGGCTGGCC 
TCCATGTACC ACAGCTGTCT GAGGAATCAG GTTGGTGATG AACACCTTAG GAAACAACAC 
CGGGTGCTGG CAGATCTTCA TTACAGCAAG GCCGCAAAGC TGTTTCAGCT GCTGAAAGAT 
GCTCCCTGCG AACTGCTTAG AGTACAGCTA GAGAGAGTAG CATTTGCTGA ATTTCAGATG 
ACCAGTCAGA ATAGCAATGT TGGAAAGTTG AAAACACTAT CTGGGGCTCT TGATATAATG 
GTGAGAACTG AGCACGCATT CCAGCTTATC CAGAAGGAGC TTATAGAAGA ATTTGGCCAG 
CCTAAGAGTG GTGACGCCGC TGCAGCTGCT GATGCTTCTC CTAGTCTCAA TCGAGAAGAA 
GTGATGAAAC TCCTCAGTAT ATTTGAGTCT CGGTTGTCAT TTCTTCTCCT TCAGTCCATT 
AAACTGCTAT CTTCAACTAA AAAGAAAACA AGCAATAACA TCGAAGATGA CACAATTCTC 
AAAACCAACA AGCACATTTA CTCCCAGCTT TTGAGAGCAA CTGCAAATAA AACCGCGACT 
CTTCTGGAAA GAATCAACGT TATCGTCCAC CTGCTGGGCC AGCTTGCCGC CGGCAGTGCA 
GCGAGCAGCA ATGCCGTTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDRF1 ( XM_017016066.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871555.1 Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu66815
Clone ID Related Accession (Same CDS sequence)	XM_017016066.1 , XM_017016066.2 , XM_011539622.1
Accession Version	XM_017016066.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	erythroid differentiation-related factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016066.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGT ACAGCTACCT CACATGCTGT TATTCCTATT TGATGGAAAG AGAGACTGAG 
GATCAAGGAG CTCAAAAATA TGAAATGATA AAGACAGAAG AAATTCCCAA TTTGGAAAAT 
TCTAATTTTT CTACTAAAGT CATAAAAGAC ATTGCACAGA ATATTTTATC ATTTTTGAAA 
TCTAATTGTA CCAAAGAAGG ACATACCTAT TGGCTCTTTA AAGCAAGTGG CAGCGATATA 
GTGAAGCTCT ATGACCTCAC TACTCTTTGT GAAGAAACTG AAGACAAATA CCAAAATCCA 
TTCACAATGC CGGTAGCCAT TCTCTTGTAC AAGGTTGCTT GCAACATGAT GATGAAGAAG 
AATCAAAATA AGAAACACTA TGGAACTATT AGAACATTGC TTCTTAATTG TCTGAAATTA 
CTGGACAAAA GTAGGCATCC TCAAATTATT GCTTCCGCCA ATTACATGCT TTCAGAACTT 
TTTCAATTGG ATGAACCTAA AAAGGAAGAA AATTCAGAAT CTCCTTTAAA TGAGAATTCT 
GATGAAAGTT ATAGTGAAGA GGAGGAAGAG ATGCCCGACA GTGATGAAAA TGGATCCTAT 
AGCACCAGCT CTGATCCATC AGATGATAGC AAAGCAGTAG CTATAATCAA GTCTGTTGGA 
GAACTATCAG TACCAGAAAA ATACAAATCT ATTCATCAAA TCAGACCCAG TTGTGCATTT 
CCAGTTTGCC ATGACACAGA AGAGCGCTGT AGACTTGTGC TTAGCTATGT TCTAGAGGGT 
TTAAAATCTG TCGATAGCAG CATCAAAAAA GAAAGCGACC TTCCAGCAGC TGACCCCAGC 
ACTCCAATCC CGTTAAAATA TGAAGATGAA TCCTCAAGAG GGGGTCCCGA GGGGCTAGAG 
AAGCAGATGG CCTTGTTTTT GGACAAAATG GGCTCCCTTC AGAAGGGCAA TTATTCCAGT 
CAATCTGGAA TGATCCCTGG CTCTTGGCAA CATAAAATGA AACTTCAGCT GATTCTCAAG 
TCATCAAAGG CCTATTATGT TTTGTCCGAT GCTGCCATGA GTCTTCAGAA ATACGGAAGA 
GCATTACGAT ACATTAAATT AGCTTTGCAA AGCCATGATA CTTATTGCTG CCTCTGCACC 
AATATGCTTT CCGAAGTGCT GTTGTTTCTC TCTCAATATT TGACACTTTG TGGTGATATC 
CAACTAATGC TGGCCCAGAA TGCAAATAAT AGAGCAGCAC ACCTTGAAGA GTTTCATTAC 
CAAACAAAAG AAGACCAGGA GATCCTGCAT AGCCTTCACA GAGAGTCCAG TTGCCAAGGA 
TTTGCATGGG CAACTGATTT GTCTACAGAC TTAGAAAGTC AACTCTCTGT TAGTTGTAAA 
TGTTATGAGG CTGCTAATGA AATCTTGCAG TTTAGTGACT TGAAAAGCCA AAATCCAGAA 
CACTATGTAC AAGTATTAAA GAGAATGGGT AACATTAGAA ATGAAATTGG TGTGTTTTAC 
ATGAATCAGG CTGCTGCATT ACAGAGTGAG AGACTAGTGA GCAAATCTGT GTCTGCTGCC 
GAGCAACAGT TGTGGAAAAA AAGCTTTTCT TGTTTTGAAA AGGGAATTCA CAACTTTGAA 
TCAATTGAGG ATGCCACCAA TGCCGCCCTT TTATTATGTA ACACGGGAAG GCTCATGCGG 
ATTTGTGCGC AGGCCCACTG TGGTGCAGGG GATGAACTGA AACGTGAATT TTCACCAGAA 
GAAGGCTTGT ATTATAATAA GGCTATTGAT TACTATTTGA AAGCGCTAAG GTCATTGGGA 
ACACGAGACA TACACCCAGC TGTTTGGGAT TCAGTGAACT GGGAATTGTC CACTACTTAC 
TTTACTATGG CAACTCTACA GCAAGATTAT GCTCCGTTAT CTAGAAAAGC TCAGGAGCAG 
ATTGAGAAAG AAGTCAGTGA GGCCATGATG AAGTCCCTAA AATACTGCGA TGTGGATTCA 
GTGTCTGCTC GACAGCCCCT TTGTCAGTAT CGAGCTGCAA CCATCCATCA CAGGCTGGCC 
TCCATGTACC ACAGCTGTCT GAGGAATCAG GTTGGTGATG AACACCTTAG GAAACAACAC 
CGGGTGCTGG CAGATCTTCA TTACAGCAAG GCCGCAAAGC TGTTTCAGCT GCTGAAAGAT 
GCTCCCTGCG AACTGCTTAG AGTACAGCTA GAGAGAGTAG CATTTGCTGA ATTTCAGATG 
ACCAGTCAGA ATAGCAATGT TGGAAAGTTG AAAACACTAT CTGGGGCTCT TGATATAATG 
GTGAGAACTG AGCACGCATT CCAGCTTATC CAGAAGGAGC TTATAGAAGA ATTTGGCCAG 
CCTAAGAGTG GTGACGCCGC TGCAGCTGCT GATGCTTCTC CTAGTCTCAA TCGAGAAGAA 
GTGATGAAAC TCCTCAGTAT ATTTGAGTCT CGGTTGTCAT TTCTTCTCCT TCAGTCCATT 
AAACTGCTAT CTTCAACTAA AAAGAAAACA AGCAATAACA TCGAAGATGA CACAATTCTC 
AAAACCAACA AGCACATTTA CTCCCAGCTT TTGAGAGCAA CTGCAAATAA AACCGCGACT 
CTTCTGGAAA GAATCAACGT TATCGTCCAC CTGCTGGGCC AGCTTGCCGC CGGCAGTGCA 
GCGAGCAGCA ATGCCGTTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871555.1
CDS	1100..3763
Translation	MELYSYLTCCYSYLMERETEDQGAQKYEMIKTEEIPNLENSNFSTKVIKDIAQNILSFLKSNCTKEGHTYWLFKASGSDIVKLYDLTTLCEETEDKYQNPFTMPVAILLYKVACNMMMKKNQNKKHYGTIRTLLLNCLKLLDKSRHPQIIASANYMLSELFQLDEPKKEENSESPLNENSDESYSEEEEEMPDSDENGSYSTSSDPSDDSKAVAIIKSVGELSVPEKYKSIHQIRPSCAFPVCHDTEERCRLVLSYVLEGLKSVDSSIKKESDLPAADPSTPIPLKYEDESSRGGPEGLEKQMALFLDKMGSLQKGNYSSQSGMIPGSWQHKMKLQLILKSSKAYYVLSDAAMSLQKYGRALRYIKLALQSHDTYCCLCTNMLSEVLLFLSQYLTLCGDIQLMLAQNANNRAAHLEEFHYQTKEDQEILHSLHRESSCQGFAWATDLSTDLESQLSVSCKCYEAANEILQFSDLKSQNPEHYVQVLKRMGNIRNEIGVFYMNQAAALQSERLVSKSVSAAEQQLWKKSFSCFEKGIHNFESIEDATNAALLLCNTGRLMRICAQAHCGAGDELKREFSPEEGLYYNKAIDYYLKALRSLGTRDIHPAVWDSVNWELSTTYFTMATLQQDYAPLSRKAQEQIEKEVSEAMMKSLKYCDVDSVSARQPLCQYRAATIHHRLASMYHSCLRNQVGDEHLRKQHRVLADLHYSKAAKLFQLLKDAPCELLRVQLERVAFAEFQMTSQNSNVGKLKTLSGALDIMVRTEHAFQLIQKELIEEFGQPKSGDAAAAADASPSLNREEVMKLLSIFESRLSFLLLQSIKLLSSTKKKTSNNIEDDTILKTNKHIYSQLLRATANKTATLLERINVIVHLLGQLAAGSAASSNAVQ

Target ORF information:

RefSeq Version	XM_017016066.2
Organism	Homo sapiens(human)
Definition	Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016066.2

ORF Insert Sequence:

ATGGAATTGT ACAGCTACCT CACATGCTGT TATTCCTATT TGATGGAAAG AGAGACTGAG 
GATCAAGGAG CTCAAAAATA TGAAATGATA AAGACAGAAG AAATTCCCAA TTTGGAAAAT 
TCTAATTTTT CTACTAAAGT CATAAAAGAC ATTGCACAGA ATATTTTATC ATTTTTGAAA 
TCTAATTGTA CCAAAGAAGG ACATACCTAT TGGCTCTTTA AAGCAAGTGG CAGCGATATA 
GTGAAGCTCT ATGACCTCAC TACTCTTTGT GAAGAAACTG AAGACAAATA CCAAAATCCA 
TTCACAATGC CGGTAGCCAT TCTCTTGTAC AAGGTTGCTT GCAACATGAT GATGAAGAAG 
AATCAAAATA AGAAACACTA TGGAACTATT AGAACATTGC TTCTTAATTG TCTGAAATTA 
CTGGACAAAA GTAGGCATCC TCAAATTATT GCTTCCGCCA ATTACATGCT TTCAGAACTT 
TTTCAATTGG ATGAACCTAA AAAGGAAGAA AATTCAGAAT CTCCTTTAAA TGAGAATTCT 
GATGAAAGTT ATAGTGAAGA GGAGGAAGAG ATGCCCGACA GTGATGAAAA TGGATCCTAT 
AGCACCAGCT CTGATCCATC AGATGATAGC AAAGCAGTAG CTATAATCAA GTCTGTTGGA 
GAACTATCAG TACCAGAAAA ATACAAATCT ATTCATCAAA TCAGACCCAG TTGTGCATTT 
CCAGTTTGCC ATGACACAGA AGAGCGCTGT AGACTTGTGC TTAGCTATGT TCTAGAGGGT 
TTAAAATCTG TCGATAGCAG CATCAAAAAA GAAAGCGACC TTCCAGCAGC TGACCCCAGC 
ACTCCAATCC CGTTAAAATA TGAAGATGAA TCCTCAAGAG GGGGTCCCGA GGGGCTAGAG 
AAGCAGATGG CCTTGTTTTT GGACAAAATG GGCTCCCTTC AGAAGGGCAA TTATTCCAGT 
CAATCTGGAA TGATCCCTGG CTCTTGGCAA CATAAAATGA AACTTCAGCT GATTCTCAAG 
TCATCAAAGG CCTATTATGT TTTGTCCGAT GCTGCCATGA GTCTTCAGAA ATACGGAAGA 
GCATTACGAT ACATTAAATT AGCTTTGCAA AGCCATGATA CTTATTGCTG CCTCTGCACC 
AATATGCTTT CCGAAGTGCT GTTGTTTCTC TCTCAATATT TGACACTTTG TGGTGATATC 
CAACTAATGC TGGCCCAGAA TGCAAATAAT AGAGCAGCAC ACCTTGAAGA GTTTCATTAC 
CAAACAAAAG AAGACCAGGA GATCCTGCAT AGCCTTCACA GAGAGTCCAG TTGCCAAGGA 
TTTGCATGGG CAACTGATTT GTCTACAGAC TTAGAAAGTC AACTCTCTGT TAGTTGTAAA 
TGTTATGAGG CTGCTAATGA AATCTTGCAG TTTAGTGACT TGAAAAGCCA AAATCCAGAA 
CACTATGTAC AAGTATTAAA GAGAATGGGT AACATTAGAA ATGAAATTGG TGTGTTTTAC 
ATGAATCAGG CTGCTGCATT ACAGAGTGAG AGACTAGTGA GCAAATCTGT GTCTGCTGCC 
GAGCAACAGT TGTGGAAAAA AAGCTTTTCT TGTTTTGAAA AGGGAATTCA CAACTTTGAA 
TCAATTGAGG ATGCCACCAA TGCCGCCCTT TTATTATGTA ACACGGGAAG GCTCATGCGG 
ATTTGTGCGC AGGCCCACTG TGGTGCAGGG GATGAACTGA AACGTGAATT TTCACCAGAA 
GAAGGCTTGT ATTATAATAA GGCTATTGAT TACTATTTGA AAGCGCTAAG GTCATTGGGA 
ACACGAGACA TACACCCAGC TGTTTGGGAT TCAGTGAACT GGGAATTGTC CACTACTTAC 
TTTACTATGG CAACTCTACA GCAAGATTAT GCTCCGTTAT CTAGAAAAGC TCAGGAGCAG 
ATTGAGAAAG AAGTCAGTGA GGCCATGATG AAGTCCCTAA AATACTGCGA TGTGGATTCA 
GTGTCTGCTC GACAGCCCCT TTGTCAGTAT CGAGCTGCAA CCATCCATCA CAGGCTGGCC 
TCCATGTACC ACAGCTGTCT GAGGAATCAG GTTGGTGATG AACACCTTAG GAAACAACAC 
CGGGTGCTGG CAGATCTTCA TTACAGCAAG GCCGCAAAGC TGTTTCAGCT GCTGAAAGAT 
GCTCCCTGCG AACTGCTTAG AGTACAGCTA GAGAGAGTAG CATTTGCTGA ATTTCAGATG 
ACCAGTCAGA ATAGCAATGT TGGAAAGTTG AAAACACTAT CTGGGGCTCT TGATATAATG 
GTGAGAACTG AGCACGCATT CCAGCTTATC CAGAAGGAGC TTATAGAAGA ATTTGGCCAG 
CCTAAGAGTG GTGACGCCGC TGCAGCTGCT GATGCTTCTC CTAGTCTCAA TCGAGAAGAA 
GTGATGAAAC TCCTCAGTAT ATTTGAGTCT CGGTTGTCAT TTCTTCTCCT TCAGTCCATT 
AAACTGCTAT CTTCAACTAA AAAGAAAACA AGCAATAACA TCGAAGATGA CACAATTCTC 
AAAACCAACA AGCACATTTA CTCCCAGCTT TTGAGAGCAA CTGCAAATAA AACCGCGACT 
CTTCTGGAAA GAATCAACGT TATCGTCCAC CTGCTGGGCC AGCTTGCCGC CGGCAGTGCA 
GCGAGCAGCA ATGCCGTTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDRF1 ( XM_011539622.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537924.1 Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X5, mRNA.

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CloneID	OHu66815
Clone ID Related Accession (Same CDS sequence)	XM_017016066.1 , XM_017016066.2 , XM_011539622.1
Accession Version	XM_011539622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	erythroid differentiation-related factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGT ACAGCTACCT CACATGCTGT TATTCCTATT TGATGGAAAG AGAGACTGAG 
GATCAAGGAG CTCAAAAATA TGAAATGATA AAGACAGAAG AAATTCCCAA TTTGGAAAAT 
TCTAATTTTT CTACTAAAGT CATAAAAGAC ATTGCACAGA ATATTTTATC ATTTTTGAAA 
TCTAATTGTA CCAAAGAAGG ACATACCTAT TGGCTCTTTA AAGCAAGTGG CAGCGATATA 
GTGAAGCTCT ATGACCTCAC TACTCTTTGT GAAGAAACTG AAGACAAATA CCAAAATCCA 
TTCACAATGC CGGTAGCCAT TCTCTTGTAC AAGGTTGCTT GCAACATGAT GATGAAGAAG 
AATCAAAATA AGAAACACTA TGGAACTATT AGAACATTGC TTCTTAATTG TCTGAAATTA 
CTGGACAAAA GTAGGCATCC TCAAATTATT GCTTCCGCCA ATTACATGCT TTCAGAACTT 
TTTCAATTGG ATGAACCTAA AAAGGAAGAA AATTCAGAAT CTCCTTTAAA TGAGAATTCT 
GATGAAAGTT ATAGTGAAGA GGAGGAAGAG ATGCCCGACA GTGATGAAAA TGGATCCTAT 
AGCACCAGCT CTGATCCATC AGATGATAGC AAAGCAGTAG CTATAATCAA GTCTGTTGGA 
GAACTATCAG TACCAGAAAA ATACAAATCT ATTCATCAAA TCAGACCCAG TTGTGCATTT 
CCAGTTTGCC ATGACACAGA AGAGCGCTGT AGACTTGTGC TTAGCTATGT TCTAGAGGGT 
TTAAAATCTG TCGATAGCAG CATCAAAAAA GAAAGCGACC TTCCAGCAGC TGACCCCAGC 
ACTCCAATCC CGTTAAAATA TGAAGATGAA TCCTCAAGAG GGGGTCCCGA GGGGCTAGAG 
AAGCAGATGG CCTTGTTTTT GGACAAAATG GGCTCCCTTC AGAAGGGCAA TTATTCCAGT 
CAATCTGGAA TGATCCCTGG CTCTTGGCAA CATAAAATGA AACTTCAGCT GATTCTCAAG 
TCATCAAAGG CCTATTATGT TTTGTCCGAT GCTGCCATGA GTCTTCAGAA ATACGGAAGA 
GCATTACGAT ACATTAAATT AGCTTTGCAA AGCCATGATA CTTATTGCTG CCTCTGCACC 
AATATGCTTT CCGAAGTGCT GTTGTTTCTC TCTCAATATT TGACACTTTG TGGTGATATC 
CAACTAATGC TGGCCCAGAA TGCAAATAAT AGAGCAGCAC ACCTTGAAGA GTTTCATTAC 
CAAACAAAAG AAGACCAGGA GATCCTGCAT AGCCTTCACA GAGAGTCCAG TTGCCAAGGA 
TTTGCATGGG CAACTGATTT GTCTACAGAC TTAGAAAGTC AACTCTCTGT TAGTTGTAAA 
TGTTATGAGG CTGCTAATGA AATCTTGCAG TTTAGTGACT TGAAAAGCCA AAATCCAGAA 
CACTATGTAC AAGTATTAAA GAGAATGGGT AACATTAGAA ATGAAATTGG TGTGTTTTAC 
ATGAATCAGG CTGCTGCATT ACAGAGTGAG AGACTAGTGA GCAAATCTGT GTCTGCTGCC 
GAGCAACAGT TGTGGAAAAA AAGCTTTTCT TGTTTTGAAA AGGGAATTCA CAACTTTGAA 
TCAATTGAGG ATGCCACCAA TGCCGCCCTT TTATTATGTA ACACGGGAAG GCTCATGCGG 
ATTTGTGCGC AGGCCCACTG TGGTGCAGGG GATGAACTGA AACGTGAATT TTCACCAGAA 
GAAGGCTTGT ATTATAATAA GGCTATTGAT TACTATTTGA AAGCGCTAAG GTCATTGGGA 
ACACGAGACA TACACCCAGC TGTTTGGGAT TCAGTGAACT GGGAATTGTC CACTACTTAC 
TTTACTATGG CAACTCTACA GCAAGATTAT GCTCCGTTAT CTAGAAAAGC TCAGGAGCAG 
ATTGAGAAAG AAGTCAGTGA GGCCATGATG AAGTCCCTAA AATACTGCGA TGTGGATTCA 
GTGTCTGCTC GACAGCCCCT TTGTCAGTAT CGAGCTGCAA CCATCCATCA CAGGCTGGCC 
TCCATGTACC ACAGCTGTCT GAGGAATCAG GTTGGTGATG AACACCTTAG GAAACAACAC 
CGGGTGCTGG CAGATCTTCA TTACAGCAAG GCCGCAAAGC TGTTTCAGCT GCTGAAAGAT 
GCTCCCTGCG AACTGCTTAG AGTACAGCTA GAGAGAGTAG CATTTGCTGA ATTTCAGATG 
ACCAGTCAGA ATAGCAATGT TGGAAAGTTG AAAACACTAT CTGGGGCTCT TGATATAATG 
GTGAGAACTG AGCACGCATT CCAGCTTATC CAGAAGGAGC TTATAGAAGA ATTTGGCCAG 
CCTAAGAGTG GTGACGCCGC TGCAGCTGCT GATGCTTCTC CTAGTCTCAA TCGAGAAGAA 
GTGATGAAAC TCCTCAGTAT ATTTGAGTCT CGGTTGTCAT TTCTTCTCCT TCAGTCCATT 
AAACTGCTAT CTTCAACTAA AAAGAAAACA AGCAATAACA TCGAAGATGA CACAATTCTC 
AAAACCAACA AGCACATTTA CTCCCAGCTT TTGAGAGCAA CTGCAAATAA AACCGCGACT 
CTTCTGGAAA GAATCAACGT TATCGTCCAC CTGCTGGGCC AGCTTGCCGC CGGCAGTGCA 
GCGAGCAGCA ATGCCGTTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537924.1
CDS	535..3198
Translation	MELYSYLTCCYSYLMERETEDQGAQKYEMIKTEEIPNLENSNFSTKVIKDIAQNILSFLKSNCTKEGHTYWLFKASGSDIVKLYDLTTLCEETEDKYQNPFTMPVAILLYKVACNMMMKKNQNKKHYGTIRTLLLNCLKLLDKSRHPQIIASANYMLSELFQLDEPKKEENSESPLNENSDESYSEEEEEMPDSDENGSYSTSSDPSDDSKAVAIIKSVGELSVPEKYKSIHQIRPSCAFPVCHDTEERCRLVLSYVLEGLKSVDSSIKKESDLPAADPSTPIPLKYEDESSRGGPEGLEKQMALFLDKMGSLQKGNYSSQSGMIPGSWQHKMKLQLILKSSKAYYVLSDAAMSLQKYGRALRYIKLALQSHDTYCCLCTNMLSEVLLFLSQYLTLCGDIQLMLAQNANNRAAHLEEFHYQTKEDQEILHSLHRESSCQGFAWATDLSTDLESQLSVSCKCYEAANEILQFSDLKSQNPEHYVQVLKRMGNIRNEIGVFYMNQAAALQSERLVSKSVSAAEQQLWKKSFSCFEKGIHNFESIEDATNAALLLCNTGRLMRICAQAHCGAGDELKREFSPEEGLYYNKAIDYYLKALRSLGTRDIHPAVWDSVNWELSTTYFTMATLQQDYAPLSRKAQEQIEKEVSEAMMKSLKYCDVDSVSARQPLCQYRAATIHHRLASMYHSCLRNQVGDEHLRKQHRVLADLHYSKAAKLFQLLKDAPCELLRVQLERVAFAEFQMTSQNSNVGKLKTLSGALDIMVRTEHAFQLIQKELIEEFGQPKSGDAAAAADASPSLNREEVMKLLSIFESRLSFLLLQSIKLLSSTKKKTSNNIEDDTILKTNKHIYSQLLRATANKTATLLERINVIVHLLGQLAAGSAASSNAVQ

Target ORF information:

RefSeq Version	XM_011539622.1
Organism	Homo sapiens(human)
Definition	Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539622.1

ORF Insert Sequence:

ATGGAATTGT ACAGCTACCT CACATGCTGT TATTCCTATT TGATGGAAAG AGAGACTGAG 
GATCAAGGAG CTCAAAAATA TGAAATGATA AAGACAGAAG AAATTCCCAA TTTGGAAAAT 
TCTAATTTTT CTACTAAAGT CATAAAAGAC ATTGCACAGA ATATTTTATC ATTTTTGAAA 
TCTAATTGTA CCAAAGAAGG ACATACCTAT TGGCTCTTTA AAGCAAGTGG CAGCGATATA 
GTGAAGCTCT ATGACCTCAC TACTCTTTGT GAAGAAACTG AAGACAAATA CCAAAATCCA 
TTCACAATGC CGGTAGCCAT TCTCTTGTAC AAGGTTGCTT GCAACATGAT GATGAAGAAG 
AATCAAAATA AGAAACACTA TGGAACTATT AGAACATTGC TTCTTAATTG TCTGAAATTA 
CTGGACAAAA GTAGGCATCC TCAAATTATT GCTTCCGCCA ATTACATGCT TTCAGAACTT 
TTTCAATTGG ATGAACCTAA AAAGGAAGAA AATTCAGAAT CTCCTTTAAA TGAGAATTCT 
GATGAAAGTT ATAGTGAAGA GGAGGAAGAG ATGCCCGACA GTGATGAAAA TGGATCCTAT 
AGCACCAGCT CTGATCCATC AGATGATAGC AAAGCAGTAG CTATAATCAA GTCTGTTGGA 
GAACTATCAG TACCAGAAAA ATACAAATCT ATTCATCAAA TCAGACCCAG TTGTGCATTT 
CCAGTTTGCC ATGACACAGA AGAGCGCTGT AGACTTGTGC TTAGCTATGT TCTAGAGGGT 
TTAAAATCTG TCGATAGCAG CATCAAAAAA GAAAGCGACC TTCCAGCAGC TGACCCCAGC 
ACTCCAATCC CGTTAAAATA TGAAGATGAA TCCTCAAGAG GGGGTCCCGA GGGGCTAGAG 
AAGCAGATGG CCTTGTTTTT GGACAAAATG GGCTCCCTTC AGAAGGGCAA TTATTCCAGT 
CAATCTGGAA TGATCCCTGG CTCTTGGCAA CATAAAATGA AACTTCAGCT GATTCTCAAG 
TCATCAAAGG CCTATTATGT TTTGTCCGAT GCTGCCATGA GTCTTCAGAA ATACGGAAGA 
GCATTACGAT ACATTAAATT AGCTTTGCAA AGCCATGATA CTTATTGCTG CCTCTGCACC 
AATATGCTTT CCGAAGTGCT GTTGTTTCTC TCTCAATATT TGACACTTTG TGGTGATATC 
CAACTAATGC TGGCCCAGAA TGCAAATAAT AGAGCAGCAC ACCTTGAAGA GTTTCATTAC 
CAAACAAAAG AAGACCAGGA GATCCTGCAT AGCCTTCACA GAGAGTCCAG TTGCCAAGGA 
TTTGCATGGG CAACTGATTT GTCTACAGAC TTAGAAAGTC AACTCTCTGT TAGTTGTAAA 
TGTTATGAGG CTGCTAATGA AATCTTGCAG TTTAGTGACT TGAAAAGCCA AAATCCAGAA 
CACTATGTAC AAGTATTAAA GAGAATGGGT AACATTAGAA ATGAAATTGG TGTGTTTTAC 
ATGAATCAGG CTGCTGCATT ACAGAGTGAG AGACTAGTGA GCAAATCTGT GTCTGCTGCC 
GAGCAACAGT TGTGGAAAAA AAGCTTTTCT TGTTTTGAAA AGGGAATTCA CAACTTTGAA 
TCAATTGAGG ATGCCACCAA TGCCGCCCTT TTATTATGTA ACACGGGAAG GCTCATGCGG 
ATTTGTGCGC AGGCCCACTG TGGTGCAGGG GATGAACTGA AACGTGAATT TTCACCAGAA 
GAAGGCTTGT ATTATAATAA GGCTATTGAT TACTATTTGA AAGCGCTAAG GTCATTGGGA 
ACACGAGACA TACACCCAGC TGTTTGGGAT TCAGTGAACT GGGAATTGTC CACTACTTAC 
TTTACTATGG CAACTCTACA GCAAGATTAT GCTCCGTTAT CTAGAAAAGC TCAGGAGCAG 
ATTGAGAAAG AAGTCAGTGA GGCCATGATG AAGTCCCTAA AATACTGCGA TGTGGATTCA 
GTGTCTGCTC GACAGCCCCT TTGTCAGTAT CGAGCTGCAA CCATCCATCA CAGGCTGGCC 
TCCATGTACC ACAGCTGTCT GAGGAATCAG GTTGGTGATG AACACCTTAG GAAACAACAC 
CGGGTGCTGG CAGATCTTCA TTACAGCAAG GCCGCAAAGC TGTTTCAGCT GCTGAAAGAT 
GCTCCCTGCG AACTGCTTAG AGTACAGCTA GAGAGAGTAG CATTTGCTGA ATTTCAGATG 
ACCAGTCAGA ATAGCAATGT TGGAAAGTTG AAAACACTAT CTGGGGCTCT TGATATAATG 
GTGAGAACTG AGCACGCATT CCAGCTTATC CAGAAGGAGC TTATAGAAGA ATTTGGCCAG 
CCTAAGAGTG GTGACGCCGC TGCAGCTGCT GATGCTTCTC CTAGTCTCAA TCGAGAAGAA 
GTGATGAAAC TCCTCAGTAT ATTTGAGTCT CGGTTGTCAT TTCTTCTCCT TCAGTCCATT 
AAACTGCTAT CTTCAACTAA AAAGAAAACA AGCAATAACA TCGAAGATGA CACAATTCTC 
AAAACCAACA AGCACATTTA CTCCCAGCTT TTGAGAGCAA CTGCAAATAA AACCGCGACT 
CTTCTGGAAA GAATCAACGT TATCGTCCAC CTGCTGGGCC AGCTTGCCGC CGGCAGTGCA 
GCGAGCAGCA ATGCCGTTCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDRF1 ( NM_015608.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_056423.2 Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08826
Clone ID Related Accession (Same CDS sequence)	NM_015608.2
Accession Version	NM_015608.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	erythroid differentiation-related factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL050102.1, AK091507.1 and BC026172.1. On Jun 13, 2003 this sequence version replaced NM_015608.1. Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.74298.1, SRR1660809.83545.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGATG CCAAGGAGGC CGGAGCCGAG GGTCCGCCGG CCGGGGCCGC CGCTCGAGGA 
GGGCTCAGCC TCCTGTCCCA GGGAGAATCC GAGGAATCTT CTGCACAGGG ATCAGCTTTA 
TTTCTTGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT 
CGAACAGCAT TTGCACGCCT TGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG 
TTACGAGAGA GTGCCAAACT AGGGCCAGCA GGAACTACCA TTCTTGGCAA CAGCAAGAAA 
AGCAAGCCAT TTTCAAGCTT TGGCATGGCA TATGACTTTA TTGATTCAGT GGGAAATGAT 
GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAACTCC TGAAAATTCC CTACAGCAAG 
TCGCACGTGA GCATGGCAGT ACACCGCATA GGAAGGACTC TCTTACTAGA TGAGCTAGAT 
ATTCAAGAAC TCTTTATGAG ATCGTCTCAG ACTGGTGACT GGACATGGTT GAAAGAGTTT 
TATCAAAGAC TCATTGATCA GAAGTGGCAG AGGAAGAAAA AGAGCAAAGA GCACTGGTAT 
CAAAAGGCAA TTCTTTCCAA GTTTTTATAT TACAGTATCA ATGGTGATGG AGCCGCTCAG 
CCTGTCTCAT CCACCGCAGA ACAGCAGGAA TCGTCCAGTT CAGATCAGAC AAATGATTCG 
GAAGGGGCTT CATGGCCTGC TCCCTTCGAA ATGCCTTCTT CAGTTTCTGA AGATCCCAGT 
GCTTCCAGTC AGGGTCTTAA AAATGATTTT GTTCGGAATA TTCTATGGAC ATTTGAAGAT 
ATCCATATGT TGGTCGGCTC CAACATGCCC ATATTTGGAG GAGGCAGATA CCCAGCAGTC 
AGCTTACGTC TCAGGGATAA CAACAAACCA ATTAATGTGC TAACTGGAAT TGACTATTGG 
TTGGACAACT TGATATGCAA TGTGCCAGAG CTTGTGATGT GTTTTCATGT AAATGGAATT 
GTACAGAAAT ATGAAATGAT AAAGACAGAA GAAATTCCCA ATTTGGAAAA TTCTAATTTT 
TCTACTAAAG TCATAAAAGA CATTGCACAG AATATTTTAT CATTTTTGAA ATCTAATTGT 
ACCAAAGAAG GACATACCTA TTGGCTCTTT AAAGCAAGTG GCAGCGATAT AGTGAAGCTC 
TATGACCTCA CTACTCTTTG TGAAGAAACT GAAGACAAAT ACCAAAATCC ATTCACAATG 
CCGGTAGCCA TTCTCTTGTA CAAGGTTGCT TGCAACATGA TGATGAAGAA GAATCAAAAT 
AAGAAACACT ATGGAACTAT TAGAACATTG CTTCTTAATT GTCTGAAATT ACTGGACAAA 
AGTAGGCATC CTCAAATTAT TGCTTCCGCC AATTACATGC TTTCAGAACT TTTTCAATTG 
GATGAACCTA AAAAGGAAGA AAATTCAGAA TCTCCTTTAA ATGAGAATTC TGATGAAAGT 
TATAGTGAAG AGGAGGAAGA GATGCCCGAC AGTGATGAAA ATGGATCCTA TAGCACCAGC 
TCTGATCCAT CAGATGATAG CAAAGCAGTA GCTATAATCA AGTCTGTTGG AGAACTATCA 
GTACCAGAAA AATACAAATC TATTCATCAA ATCAGACCCA GTTGTGCATT TCCAGTTTGC 
CATGACACAG AAGAGCGCTG TAGACTTGTG CTTAGCTATG TTCTAGAGGG TTTAAAATCT 
GTCGATAGCA GCATCAAAAA AGAAAGCGAC CTTCCAGCAG CTGACCCCAG CACTCCAATC 
CCGTTAAAAT ATGAAGATGA ATCCTCAAGA GGGGGTCCCG AGGGGCTAGA GAAGCAGATG 
GCCTTGTTTT TGGACAAAAT GGGCTCCCTT CAGAAGGGCA ATTATTCCAG TCAATCTGGA 
ATGATCCCTG GCTCTTGGCA ACATAAAATG AAACTTCAGC TGATTCTCAA GTCATCAAAG 
GCCTATTATG TTTTGTCCGA TGCTGCCATG AGTCTTCAGA AATACGGAAG AGCATTACGA 
TACATTAAAT TAGCTTTGCA AAGCCATGAT ACTTATTGCT GCCTCTGCAC CAATATGCTT 
TCCGAAGTGC TGTTGTTTCT CTCTCAATAT TTGACACTTT GTGGTGATAT CCAACTAATG 
CTGGCCCAGA ATGCAAATAA TAGAGCAGCA CACCTTGAAG AGTTTCATTA CCAAACAAAA 
GAAGACCAGG AGATCCTGCA TAGCCTTCAC AGAGAGTCCA GTTGCCAAGG ATTTGCATGG 
GCAACTGATT TGTCTACAGA CTTAGAAAGT CAACTCTCTG TTAGTTGTAA ATGTTATGAG 
GCTGCTAATG AAATCTTGCA GTTTAGTGAC TTGAAAAGCC AAAATCCAGA ACACTATGTA 
CAAGTATTAA AGAGAATGGG TAACATTAGA AATGAAATTG GTGTGTTTTA CATGAATCAG 
GCTGCTGCAT TACAGAGTGA GAGACTAGTG AGCAAATCTG TGTCTGCTGC CGAGCAACAG 
TTGTGGAAAA AAAGCTTTTC TTGTTTTGAA AAGGGAATTC ACAACTTTGA ATCAATTGAG 
GATGCCACCA ATGCCGCCCT TTTATTATGT AACACGGGAA GGCTCATGCG GATTTGTGCG 
CAGGCCCACT GTGGTGCAGG GGATGAACTG AAACGTGAAT TTTCACCAGA AGAAGGCTTG 
TATTATAATA AGGCTATTGA TTACTATTTG AAAGCGCTAA GGTCATTGGG AACACGAGAC 
ATACACCCAG CTGTTTGGGA TTCAGTGAAC TGGGAATTGT CCACTACTTA CTTTACTATG 
GCAACTCTAC AGCAAGATTA TGCTCCGTTA TCTAGAAAAG CTCAGGAGCA GATTGAGAAA 
GAAGTCAGTG AGGCCATGAT GAAGTCCCTA AAATACTGCG ATGTGGATTC AGTGTCTGCT 
CGACAGCCCC TTTGTCAGTA TCGAGCTGCA ACCATCCATC ACAGGCTGGC CTCCATGTAC 
CACAGCTGTC TGAGGAATCA GGTTGGTGAT GAACACCTTA GGAAACAACA CCGGGTGCTG 
GCAGATCTTC ATTACAGCAA GGCCGCAAAG CTGTTTCAGC TGCTGAAAGA TGCTCCCTGC 
GAACTGCTTA GAGTACAGCT AGAGAGAGTA GCATTTGCTG AATTTCAGAT GACCAGTCAG 
AATAGCAATG TTGGAAAGTT GAAAACACTA TCTGGGGCTC TTGATATAAT GGTGAGAACT 
GAGCACGCAT TCCAGCTTAT CCAGAAGGAG CTTATAGAAG AATTTGGCCA GCCTAAGAGT 
GGTGACGCCG CTGCAGCTGC TGATGCTTCT CCTAGTCTCA ATCGAGAAGA AGTGATGAAA 
CTCCTCAGTA TATTTGAGTC TCGGTTGTCA TTTCTTCTCC TTCAGTCCAT TAAACTGCTA 
TCTTCAACTA AAAAGAAAAC AAGCAATAAC ATCGAAGATG ACACAATTCT CAAAACCAAC 
AAGCACATTT ACTCCCAGCT TTTGAGAGCA ACTGCAAATA AAACCGCGAC TCTTCTGGAA 
AGAATCAACG TTATCGTCCA CCTGCTGGGC CAGCTTGCCG CCGGCAGTGC AGCGAGCAGC 
AATGCCGTTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056423.2
CDS	294..3908
Translation	MGDAKEAGAEGPPAGAAARGGLSLLSQGESEESSAQGSALFLGGNEVKSRAVVKYSSAPPRTAFARLEEKTDLKLPPANWLRESAKLGPAGTTILGNSKKSKPFSSFGMAYDFIDSVGNDVDVVSDSENIKKLLKIPYSKSHVSMAVHRIGRTLLLDELDIQELFMRSSQTGDWTWLKEFYQRLIDQKWQRKKKSKEHWYQKAILSKFLYYSINGDGAAQPVSSTAEQQESSSSDQTNDSEGASWPAPFEMPSSVSEDPSASSQGLKNDFVRNILWTFEDIHMLVGSNMPIFGGGRYPAVSLRLRDNNKPINVLTGIDYWLDNLICNVPELVMCFHVNGIVQKYEMIKTEEIPNLENSNFSTKVIKDIAQNILSFLKSNCTKEGHTYWLFKASGSDIVKLYDLTTLCEETEDKYQNPFTMPVAILLYKVACNMMMKKNQNKKHYGTIRTLLLNCLKLLDKSRHPQIIASANYMLSELFQLDEPKKEENSESPLNENSDESYSEEEEEMPDSDENGSYSTSSDPSDDSKAVAIIKSVGELSVPEKYKSIHQIRPSCAFPVCHDTEERCRLVLSYVLEGLKSVDSSIKKESDLPAADPSTPIPLKYEDESSRGGPEGLEKQMALFLDKMGSLQKGNYSSQSGMIPGSWQHKMKLQLILKSSKAYYVLSDAAMSLQKYGRALRYIKLALQSHDTYCCLCTNMLSEVLLFLSQYLTLCGDIQLMLAQNANNRAAHLEEFHYQTKEDQEILHSLHRESSCQGFAWATDLSTDLESQLSVSCKCYEAANEILQFSDLKSQNPEHYVQVLKRMGNIRNEIGVFYMNQAAALQSERLVSKSVSAAEQQLWKKSFSCFEKGIHNFESIEDATNAALLLCNTGRLMRICAQAHCGAGDELKREFSPEEGLYYNKAIDYYLKALRSLGTRDIHPAVWDSVNWELSTTYFTMATLQQDYAPLSRKAQEQIEKEVSEAMMKSLKYCDVDSVSARQPLCQYRAATIHHRLASMYHSCLRNQVGDEHLRKQHRVLADLHYSKAAKLFQLLKDAPCELLRVQLERVAFAEFQMTSQNSNVGKLKTLSGALDIMVRTEHAFQLIQKELIEEFGQPKSGDAAAAADASPSLNREEVMKLLSIFESRLSFLLLQSIKLLSSTKKKTSNNIEDDTILKTNKHIYSQLLRATANKTATLLERINVIVHLLGQLAAGSAASSNAVQ

Target ORF information:

RefSeq Version	NM_015608.2
Organism	Homo sapiens(human)
Definition	Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015608.2

ORF Insert Sequence:

ATGGGGGATG CCAAGGAGGC CGGAGCCGAG GGTCCGCCGG CCGGGGCCGC CGCTCGAGGA 
GGGCTCAGCC TCCTGTCCCA GGGAGAATCC GAGGAATCTT CTGCACAGGG ATCAGCTTTA 
TTTCTTGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT 
CGAACAGCAT TTGCACGCCT TGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG 
TTACGAGAGA GTGCCAAACT AGGGCCAGCA GGAACTACCA TTCTTGGCAA CAGCAAGAAA 
AGCAAGCCAT TTTCAAGCTT TGGCATGGCA TATGACTTTA TTGATTCAGT GGGAAATGAT 
GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAACTCC TGAAAATTCC CTACAGCAAG 
TCGCACGTGA GCATGGCAGT ACACCGCATA GGAAGGACTC TCTTACTAGA TGAGCTAGAT 
ATTCAAGAAC TCTTTATGAG ATCGTCTCAG ACTGGTGACT GGACATGGTT GAAAGAGTTT 
TATCAAAGAC TCATTGATCA GAAGTGGCAG AGGAAGAAAA AGAGCAAAGA GCACTGGTAT 
CAAAAGGCAA TTCTTTCCAA GTTTTTATAT TACAGTATCA ATGGTGATGG AGCCGCTCAG 
CCTGTCTCAT CCACCGCAGA ACAGCAGGAA TCGTCCAGTT CAGATCAGAC AAATGATTCG 
GAAGGGGCTT CATGGCCTGC TCCCTTCGAA ATGCCTTCTT CAGTTTCTGA AGATCCCAGT 
GCTTCCAGTC AGGGTCTTAA AAATGATTTT GTTCGGAATA TTCTATGGAC ATTTGAAGAT 
ATCCATATGT TGGTCGGCTC CAACATGCCC ATATTTGGAG GAGGCAGATA CCCAGCAGTC 
AGCTTACGTC TCAGGGATAA CAACAAACCA ATTAATGTGC TAACTGGAAT TGACTATTGG 
TTGGACAACT TGATATGCAA TGTGCCAGAG CTTGTGATGT GTTTTCATGT AAATGGAATT 
GTACAGAAAT ATGAAATGAT AAAGACAGAA GAAATTCCCA ATTTGGAAAA TTCTAATTTT 
TCTACTAAAG TCATAAAAGA CATTGCACAG AATATTTTAT CATTTTTGAA ATCTAATTGT 
ACCAAAGAAG GACATACCTA TTGGCTCTTT AAAGCAAGTG GCAGCGATAT AGTGAAGCTC 
TATGACCTCA CTACTCTTTG TGAAGAAACT GAAGACAAAT ACCAAAATCC ATTCACAATG 
CCGGTAGCCA TTCTCTTGTA CAAGGTTGCT TGCAACATGA TGATGAAGAA GAATCAAAAT 
AAGAAACACT ATGGAACTAT TAGAACATTG CTTCTTAATT GTCTGAAATT ACTGGACAAA 
AGTAGGCATC CTCAAATTAT TGCTTCCGCC AATTACATGC TTTCAGAACT TTTTCAATTG 
GATGAACCTA AAAAGGAAGA AAATTCAGAA TCTCCTTTAA ATGAGAATTC TGATGAAAGT 
TATAGTGAAG AGGAGGAAGA GATGCCCGAC AGTGATGAAA ATGGATCCTA TAGCACCAGC 
TCTGATCCAT CAGATGATAG CAAAGCAGTA GCTATAATCA AGTCTGTTGG AGAACTATCA 
GTACCAGAAA AATACAAATC TATTCATCAA ATCAGACCCA GTTGTGCATT TCCAGTTTGC 
CATGACACAG AAGAGCGCTG TAGACTTGTG CTTAGCTATG TTCTAGAGGG TTTAAAATCT 
GTCGATAGCA GCATCAAAAA AGAAAGCGAC CTTCCAGCAG CTGACCCCAG CACTCCAATC 
CCGTTAAAAT ATGAAGATGA ATCCTCAAGA GGGGGTCCCG AGGGGCTAGA GAAGCAGATG 
GCCTTGTTTT TGGACAAAAT GGGCTCCCTT CAGAAGGGCA ATTATTCCAG TCAATCTGGA 
ATGATCCCTG GCTCTTGGCA ACATAAAATG AAACTTCAGC TGATTCTCAA GTCATCAAAG 
GCCTATTATG TTTTGTCCGA TGCTGCCATG AGTCTTCAGA AATACGGAAG AGCATTACGA 
TACATTAAAT TAGCTTTGCA AAGCCATGAT ACTTATTGCT GCCTCTGCAC CAATATGCTT 
TCCGAAGTGC TGTTGTTTCT CTCTCAATAT TTGACACTTT GTGGTGATAT CCAACTAATG 
CTGGCCCAGA ATGCAAATAA TAGAGCAGCA CACCTTGAAG AGTTTCATTA CCAAACAAAA 
GAAGACCAGG AGATCCTGCA TAGCCTTCAC AGAGAGTCCA GTTGCCAAGG ATTTGCATGG 
GCAACTGATT TGTCTACAGA CTTAGAAAGT CAACTCTCTG TTAGTTGTAA ATGTTATGAG 
GCTGCTAATG AAATCTTGCA GTTTAGTGAC TTGAAAAGCC AAAATCCAGA ACACTATGTA 
CAAGTATTAA AGAGAATGGG TAACATTAGA AATGAAATTG GTGTGTTTTA CATGAATCAG 
GCTGCTGCAT TACAGAGTGA GAGACTAGTG AGCAAATCTG TGTCTGCTGC CGAGCAACAG 
TTGTGGAAAA AAAGCTTTTC TTGTTTTGAA AAGGGAATTC ACAACTTTGA ATCAATTGAG 
GATGCCACCA ATGCCGCCCT TTTATTATGT AACACGGGAA GGCTCATGCG GATTTGTGCG 
CAGGCCCACT GTGGTGCAGG GGATGAACTG AAACGTGAAT TTTCACCAGA AGAAGGCTTG 
TATTATAATA AGGCTATTGA TTACTATTTG AAAGCGCTAA GGTCATTGGG AACACGAGAC 
ATACACCCAG CTGTTTGGGA TTCAGTGAAC TGGGAATTGT CCACTACTTA CTTTACTATG 
GCAACTCTAC AGCAAGATTA TGCTCCGTTA TCTAGAAAAG CTCAGGAGCA GATTGAGAAA 
GAAGTCAGTG AGGCCATGAT GAAGTCCCTA AAATACTGCG ATGTGGATTC AGTGTCTGCT 
CGACAGCCCC TTTGTCAGTA TCGAGCTGCA ACCATCCATC ACAGGCTGGC CTCCATGTAC 
CACAGCTGTC TGAGGAATCA GGTTGGTGAT GAACACCTTA GGAAACAACA CCGGGTGCTG 
GCAGATCTTC ATTACAGCAA GGCCGCAAAG CTGTTTCAGC TGCTGAAAGA TGCTCCCTGC 
GAACTGCTTA GAGTACAGCT AGAGAGAGTA GCATTTGCTG AATTTCAGAT GACCAGTCAG 
AATAGCAATG TTGGAAAGTT GAAAACACTA TCTGGGGCTC TTGATATAAT GGTGAGAACT 
GAGCACGCAT TCCAGCTTAT CCAGAAGGAG CTTATAGAAG AATTTGGCCA GCCTAAGAGT 
GGTGACGCCG CTGCAGCTGC TGATGCTTCT CCTAGTCTCA ATCGAGAAGA AGTGATGAAA 
CTCCTCAGTA TATTTGAGTC TCGGTTGTCA TTTCTTCTCC TTCAGTCCAT TAAACTGCTA 
TCTTCAACTA AAAAGAAAAC AAGCAATAAC ATCGAAGATG ACACAATTCT CAAAACCAAC 
AAGCACATTT ACTCCCAGCT TTTGAGAGCA ACTGCAAATA AAACCGCGAC TCTTCTGGAA 
AGAATCAACG TTATCGTCCA CCTGCTGGGC CAGCTTGCCG CCGGCAGTGC AGCGAGCAGC 
AATGCCGTTC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDRF1 ( NM_001202438.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001189367.1 Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09248
Clone ID Related Accession (Same CDS sequence)	NM_001202438.1 , NM_001202438.2
Accession Version	NM_001202438.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	erythroid differentiation-related factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC026172.1, AL158835.11, BC107479.1 and AL050102.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.189997.1, SRR1660805.125095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGATG CCAAGGAGGC CGGAGCCGAG GGTCCGCCGG CCGGGGCCGC CGCTCGAGGA 
GGGCTCAGCC TCCTGTCCCA GGGAGAATCC GAGGAATCTT CTGCACAGGG ATCAGCTTTA 
TTTCTTGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT 
CGAACAGCAT TTGCACGCCT TGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG 
TTACGAGAGA GTGCCAAACT AGGGCCAGCA GGAACTACCA TTCTTGGCAA CAGCAAGAAA 
AGCAAGCCAT TTTCAAGCTT TGGCATGGCA TATGACTTTA TTGATTCAGT GGGAAATGAT 
GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAACTCC TGAAAATTCC CTACAGCAAG 
TCGCACGTGA GCATGGCAGT ACACCGCATA GGAAGGACTC TCTTACTAGA TGAGCTAGAT 
ATTCAAGAAC TCTTTATGAG ATCGTCTCAG ACTGGTGACT GGACATGGTT GAAAGAGTTT 
TATCAAAGAC TCATTGATCA GAAGTGGCAG AGGAAGAAAA AGAGCAAAGA GCACTGGTAT 
CAAAAGGCAA TTCTTTCCAA GTTTTTATAT TACAGTATCA ATGGTGATGG AGCCGCTCAG 
CCTGTCTCAT CCACCGCAGA ACAGCAGGAA TCGTCCAGTT CAGATCAGAC AAATGATTCG 
GAAGGGGCTT CATGGCCTGC TCCCTTCGAA ATGCCTTCTT CAGTTTCTGA AGATCCCAGT 
GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA 
GCCCCCAAAG AACAAAACCT GATTACTTTA TTCAATGACG GGGAGCACAG TCAGGGTCTT 
AAAAATGATT TTGTTCGGAA TATTCTATGG ACATTTGAAG ATATCCATAT GTTGGTCGGC 
TCCAACATGC CCATATTTGG AGGAGGCAGA TACCCAGCAG TCAGCTTACG TCTCAGGGAT 
AACAACAAAC CAATTAATGT GCTAACTGGA ATTGACTATT GGTTGGACAA CTTGATATGC 
AATGTGCCAG AGCTTGTGAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA ATATGAAATG 
ATAAAGACAG AAGAAATTCC CAATTTGGAA AATTCTAATT TTTCTACTAA AGTCATAAAA 
GACATTGCAC AGAATATTTT ATCATTTTTG AAATCTAATT GTACCAAAGA AGGACATACC 
TATTGGCTCT TTAAAGCAAG TGGCAGCGAT ATAGTGAAGC TCTATGACCT CACTACTCTT 
TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCGGTAGC CATTCTCTTG 
TACAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTATGGAACT 
ATTAGAACAT TGCTTCTTAA TTGTCTGAAA TTACTGGACA AAAGTAGGCA TCCTCAAATT 
ATTGCTTCCG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAGGAA 
GAAAATTCAG AATCTCCTTT AAATGAGAAT TCTGATGAAA GTTATAGTGA AGAGGAGGAA 
GAGATGCCCG ACAGTGATGA AAATGGATCC TATAGCACCA GCTCTGATCC ATCAGATGAT 
AGCAAAGCAG TAGCTATAAT CAAGTCTGTT GGAGAACTAT CAGTACCAGA AAAATACAAA 
TCTATTCATC AAATCAGACC CAGTTGTGCA TTTCCAGTTT GCCATGACAC AGAAGAGCGC 
TGTAGACTTG TGCTTAGCTA TGTTCTAGAG GGTTTAAAAT CTGTCGATAG CAGCATCAAA 
AAAGAAAGCG ACCTTCCAGC AGCTGACCCC AGCACTCCAA TCCCGTTAAA ATATGAAGAT 
GAATCCTCAA GAGGGGGTCC CGAGGGGCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA 
ATGGGCTCCC TTCAGAAGGG CAATTATTCC AGTCAATCTG GAATGATCCC TGGCTCTTGG 
CAACATAAAA TGAAACTTCA GCTGATTCTC AAGTCATCAA AGGCCTATTA TGTTTTGTCC 
GATGCTGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG 
CAAAGCCATG ATACTTATTG CTGCCTCTGC ACCAATATGC TTTCCGAAGT GCTGTTGTTT 
CTCTCTCAAT ATTTGACACT TTGTGGTGAT ATCCAACTAA TGCTGGCCCA GAATGCAAAT 
AATAGAGCAG CACACCTTGA AGAGTTTCAT TACCAAACAA AAGAAGACCA GGAGATCCTG 
CATAGCCTTC ACAGAGAGTC CAGTTGCCAA GGATTTGCAT GGGCAACTGA TTTGTCTACA 
GACTTAGAAA GTCAACTCTC TGTTAGTTGT AAATGTTATG AGGCTGCTAA TGAAATCTTG 
CAGTTTAGTG ACTTGAAAAG CCAAAATCCA GAACACTATG TACAAGTATT AAAGAGAATG 
GGTAACATTA GAAATGAAAT TGGTGTGTTT TACATGAATC AGGCTGCTGC ATTACAGAGT 
GAGAGACTAG TGAGCAAATC TGTGTCTGCT GCCGAGCAAC AGTTGTGGAA AAAAAGCTTT 
TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAATCAATTG AGGATGCCAC CAATGCCGCC 
CTTTTATTAT GTAACACGGG AAGGCTCATG CGGATTTGTG CGCAGGCCCA CTGTGGTGCA 
GGGGATGAAC TGAAACGTGA ATTTTCACCA GAAGAAGGCT TGTATTATAA TAAGGCTATT 
GATTACTATT TGAAAGCGCT AAGGTCATTG GGAACACGAG ACATACACCC AGCTGTTTGG 
GATTCAGTGA ACTGGGAATT GTCCACTACT TACTTTACTA TGGCAACTCT ACAGCAAGAT 
TATGCTCCGT TATCTAGAAA AGCTCAGGAG CAGATTGAGA AAGAAGTCAG TGAGGCCATG 
ATGAAGTCCC TAAAATACTG CGATGTGGAT TCAGTGTCTG CTCGACAGCC CCTTTGTCAG 
TATCGAGCTG CAACCATCCA TCACAGGCTG GCCTCCATGT ACCACAGCTG TCTGAGGAAT 
CAGGTTGGTG ATGAACACCT TAGGAAACAA CACCGGGTGC TGGCAGATCT TCATTACAGC 
AAGGCCGCAA AGCTGTTTCA GCTGCTGAAA GATGCTCCCT GCGAACTGCT TAGAGTACAG 
CTAGAGAGAG TAGCATTTGC TGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGAAAG 
TTGAAAACAC TATCTGGGGC TCTTGATATA ATGGTGAGAA CTGAGCACGC ATTCCAGCTT 
ATCCAGAAGG AGCTTATAGA AGAATTTGGC CAGCCTAAGA GTGGTGACGC CGCTGCAGCT 
GCTGATGCTT CTCCTAGTCT CAATCGAGAA GAAGTGATGA AACTCCTCAG TATATTTGAG 
TCTCGGTTGT CATTTCTTCT CCTTCAGTCC ATTAAACTGC TATCTTCAAC TAAAAAGAAA 
ACAAGCAATA ACATCGAAGA TGACACAATT CTCAAAACCA ACAAGCACAT TTACTCCCAG 
CTTTTGAGAG CAACTGCAAA TAAAACCGCG ACTCTTCTGG AAAGAATCAA CGTTATCGTC 
CACCTGCTGG GCCAGCTTGC CGCCGGCAGT GCAGCGAGCA GCAATGCCGT TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189367.1
CDS	294..4010
Translation	MGDAKEAGAEGPPAGAAARGGLSLLSQGESEESSAQGSALFLGGNEVKSRAVVKYSSAPPRTAFARLEEKTDLKLPPANWLRESAKLGPAGTTILGNSKKSKPFSSFGMAYDFIDSVGNDVDVVSDSENIKKLLKIPYSKSHVSMAVHRIGRTLLLDELDIQELFMRSSQTGDWTWLKEFYQRLIDQKWQRKKKSKEHWYQKAILSKFLYYSINGDGAAQPVSSTAEQQESSSSDQTNDSEGASWPAPFEMPSSVSEDPSASSQGSEPLEPSYIVGHVASAPKEQNLITLFNDGEHSQGLKNDFVRNILWTFEDIHMLVGSNMPIFGGGRYPAVSLRLRDNNKPINVLTGIDYWLDNLICNVPELVMCFHVNGIVQKYEMIKTEEIPNLENSNFSTKVIKDIAQNILSFLKSNCTKEGHTYWLFKASGSDIVKLYDLTTLCEETEDKYQNPFTMPVAILLYKVACNMMMKKNQNKKHYGTIRTLLLNCLKLLDKSRHPQIIASANYMLSELFQLDEPKKEENSESPLNENSDESYSEEEEEMPDSDENGSYSTSSDPSDDSKAVAIIKSVGELSVPEKYKSIHQIRPSCAFPVCHDTEERCRLVLSYVLEGLKSVDSSIKKESDLPAADPSTPIPLKYEDESSRGGPEGLEKQMALFLDKMGSLQKGNYSSQSGMIPGSWQHKMKLQLILKSSKAYYVLSDAAMSLQKYGRALRYIKLALQSHDTYCCLCTNMLSEVLLFLSQYLTLCGDIQLMLAQNANNRAAHLEEFHYQTKEDQEILHSLHRESSCQGFAWATDLSTDLESQLSVSCKCYEAANEILQFSDLKSQNPEHYVQVLKRMGNIRNEIGVFYMNQAAALQSERLVSKSVSAAEQQLWKKSFSCFEKGIHNFESIEDATNAALLLCNTGRLMRICAQAHCGAGDELKREFSPEEGLYYNKAIDYYLKALRSLGTRDIHPAVWDSVNWELSTTYFTMATLQQDYAPLSRKAQEQIEKEVSEAMMKSLKYCDVDSVSARQPLCQYRAATIHHRLASMYHSCLRNQVGDEHLRKQHRVLADLHYSKAAKLFQLLKDAPCELLRVQLERVAFAEFQMTSQNSNVGKLKTLSGALDIMVRTEHAFQLIQKELIEEFGQPKSGDAAAAADASPSLNREEVMKLLSIFESRLSFLLLQSIKLLSSTKKKTSNNIEDDTILKTNKHIYSQLLRATANKTATLLERINVIVHLLGQLAAGSAASSNAVQ

Target ORF information:

RefSeq Version	NM_001202438.1
Organism	Homo sapiens(human)
Definition	Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202438.1

ORF Insert Sequence:

ATGGGGGATG CCAAGGAGGC CGGAGCCGAG GGTCCGCCGG CCGGGGCCGC CGCTCGAGGA 
GGGCTCAGCC TCCTGTCCCA GGGAGAATCC GAGGAATCTT CTGCACAGGG ATCAGCTTTA 
TTTCTTGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT 
CGAACAGCAT TTGCACGCCT TGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG 
TTACGAGAGA GTGCCAAACT AGGGCCAGCA GGAACTACCA TTCTTGGCAA CAGCAAGAAA 
AGCAAGCCAT TTTCAAGCTT TGGCATGGCA TATGACTTTA TTGATTCAGT GGGAAATGAT 
GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAACTCC TGAAAATTCC CTACAGCAAG 
TCGCACGTGA GCATGGCAGT ACACCGCATA GGAAGGACTC TCTTACTAGA TGAGCTAGAT 
ATTCAAGAAC TCTTTATGAG ATCGTCTCAG ACTGGTGACT GGACATGGTT GAAAGAGTTT 
TATCAAAGAC TCATTGATCA GAAGTGGCAG AGGAAGAAAA AGAGCAAAGA GCACTGGTAT 
CAAAAGGCAA TTCTTTCCAA GTTTTTATAT TACAGTATCA ATGGTGATGG AGCCGCTCAG 
CCTGTCTCAT CCACCGCAGA ACAGCAGGAA TCGTCCAGTT CAGATCAGAC AAATGATTCG 
GAAGGGGCTT CATGGCCTGC TCCCTTCGAA ATGCCTTCTT CAGTTTCTGA AGATCCCAGT 
GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA 
GCCCCCAAAG AACAAAACCT GATTACTTTA TTCAATGACG GGGAGCACAG TCAGGGTCTT 
AAAAATGATT TTGTTCGGAA TATTCTATGG ACATTTGAAG ATATCCATAT GTTGGTCGGC 
TCCAACATGC CCATATTTGG AGGAGGCAGA TACCCAGCAG TCAGCTTACG TCTCAGGGAT 
AACAACAAAC CAATTAATGT GCTAACTGGA ATTGACTATT GGTTGGACAA CTTGATATGC 
AATGTGCCAG AGCTTGTGAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA ATATGAAATG 
ATAAAGACAG AAGAAATTCC CAATTTGGAA AATTCTAATT TTTCTACTAA AGTCATAAAA 
GACATTGCAC AGAATATTTT ATCATTTTTG AAATCTAATT GTACCAAAGA AGGACATACC 
TATTGGCTCT TTAAAGCAAG TGGCAGCGAT ATAGTGAAGC TCTATGACCT CACTACTCTT 
TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCGGTAGC CATTCTCTTG 
TACAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTATGGAACT 
ATTAGAACAT TGCTTCTTAA TTGTCTGAAA TTACTGGACA AAAGTAGGCA TCCTCAAATT 
ATTGCTTCCG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAGGAA 
GAAAATTCAG AATCTCCTTT AAATGAGAAT TCTGATGAAA GTTATAGTGA AGAGGAGGAA 
GAGATGCCCG ACAGTGATGA AAATGGATCC TATAGCACCA GCTCTGATCC ATCAGATGAT 
AGCAAAGCAG TAGCTATAAT CAAGTCTGTT GGAGAACTAT CAGTACCAGA AAAATACAAA 
TCTATTCATC AAATCAGACC CAGTTGTGCA TTTCCAGTTT GCCATGACAC AGAAGAGCGC 
TGTAGACTTG TGCTTAGCTA TGTTCTAGAG GGTTTAAAAT CTGTCGATAG CAGCATCAAA 
AAAGAAAGCG ACCTTCCAGC AGCTGACCCC AGCACTCCAA TCCCGTTAAA ATATGAAGAT 
GAATCCTCAA GAGGGGGTCC CGAGGGGCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA 
ATGGGCTCCC TTCAGAAGGG CAATTATTCC AGTCAATCTG GAATGATCCC TGGCTCTTGG 
CAACATAAAA TGAAACTTCA GCTGATTCTC AAGTCATCAA AGGCCTATTA TGTTTTGTCC 
GATGCTGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG 
CAAAGCCATG ATACTTATTG CTGCCTCTGC ACCAATATGC TTTCCGAAGT GCTGTTGTTT 
CTCTCTCAAT ATTTGACACT TTGTGGTGAT ATCCAACTAA TGCTGGCCCA GAATGCAAAT 
AATAGAGCAG CACACCTTGA AGAGTTTCAT TACCAAACAA AAGAAGACCA GGAGATCCTG 
CATAGCCTTC ACAGAGAGTC CAGTTGCCAA GGATTTGCAT GGGCAACTGA TTTGTCTACA 
GACTTAGAAA GTCAACTCTC TGTTAGTTGT AAATGTTATG AGGCTGCTAA TGAAATCTTG 
CAGTTTAGTG ACTTGAAAAG CCAAAATCCA GAACACTATG TACAAGTATT AAAGAGAATG 
GGTAACATTA GAAATGAAAT TGGTGTGTTT TACATGAATC AGGCTGCTGC ATTACAGAGT 
GAGAGACTAG TGAGCAAATC TGTGTCTGCT GCCGAGCAAC AGTTGTGGAA AAAAAGCTTT 
TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAATCAATTG AGGATGCCAC CAATGCCGCC 
CTTTTATTAT GTAACACGGG AAGGCTCATG CGGATTTGTG CGCAGGCCCA CTGTGGTGCA 
GGGGATGAAC TGAAACGTGA ATTTTCACCA GAAGAAGGCT TGTATTATAA TAAGGCTATT 
GATTACTATT TGAAAGCGCT AAGGTCATTG GGAACACGAG ACATACACCC AGCTGTTTGG 
GATTCAGTGA ACTGGGAATT GTCCACTACT TACTTTACTA TGGCAACTCT ACAGCAAGAT 
TATGCTCCGT TATCTAGAAA AGCTCAGGAG CAGATTGAGA AAGAAGTCAG TGAGGCCATG 
ATGAAGTCCC TAAAATACTG CGATGTGGAT TCAGTGTCTG CTCGACAGCC CCTTTGTCAG 
TATCGAGCTG CAACCATCCA TCACAGGCTG GCCTCCATGT ACCACAGCTG TCTGAGGAAT 
CAGGTTGGTG ATGAACACCT TAGGAAACAA CACCGGGTGC TGGCAGATCT TCATTACAGC 
AAGGCCGCAA AGCTGTTTCA GCTGCTGAAA GATGCTCCCT GCGAACTGCT TAGAGTACAG 
CTAGAGAGAG TAGCATTTGC TGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGAAAG 
TTGAAAACAC TATCTGGGGC TCTTGATATA ATGGTGAGAA CTGAGCACGC ATTCCAGCTT 
ATCCAGAAGG AGCTTATAGA AGAATTTGGC CAGCCTAAGA GTGGTGACGC CGCTGCAGCT 
GCTGATGCTT CTCCTAGTCT CAATCGAGAA GAAGTGATGA AACTCCTCAG TATATTTGAG 
TCTCGGTTGT CATTTCTTCT CCTTCAGTCC ATTAAACTGC TATCTTCAAC TAAAAAGAAA 
ACAAGCAATA ACATCGAAGA TGACACAATT CTCAAAACCA ACAAGCACAT TTACTCCCAG 
CTTTTGAGAG CAACTGCAAA TAAAACCGCG ACTCTTCTGG AAAGAATCAA CGTTATCGTC 
CACCTGCTGG GCCAGCTTGC CGCCGGCAGT GCAGCGAGCA GCAATGCCGT TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDRF1 ( NM_001202438.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001189367.1 Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09248
Clone ID Related Accession (Same CDS sequence)	NM_001202438.1 , NM_001202438.2
Accession Version	NM_001202438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	erythroid differentiation-related factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC026172.1, BC107479.1 and AL050102.1. On Nov 22, 2018 this sequence version replaced NM_001202438.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.189997.1, SRR1660805.125095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000356792.9/ ENSP00000349244.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGGATG CCAAGGAGGC CGGAGCCGAG GGTCCGCCGG CCGGGGCCGC CGCTCGAGGA 
GGGCTCAGCC TCCTGTCCCA GGGAGAATCC GAGGAATCTT CTGCACAGGG ATCAGCTTTA 
TTTCTTGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT 
CGAACAGCAT TTGCACGCCT TGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG 
TTACGAGAGA GTGCCAAACT AGGGCCAGCA GGAACTACCA TTCTTGGCAA CAGCAAGAAA 
AGCAAGCCAT TTTCAAGCTT TGGCATGGCA TATGACTTTA TTGATTCAGT GGGAAATGAT 
GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAACTCC TGAAAATTCC CTACAGCAAG 
TCGCACGTGA GCATGGCAGT ACACCGCATA GGAAGGACTC TCTTACTAGA TGAGCTAGAT 
ATTCAAGAAC TCTTTATGAG ATCGTCTCAG ACTGGTGACT GGACATGGTT GAAAGAGTTT 
TATCAAAGAC TCATTGATCA GAAGTGGCAG AGGAAGAAAA AGAGCAAAGA GCACTGGTAT 
CAAAAGGCAA TTCTTTCCAA GTTTTTATAT TACAGTATCA ATGGTGATGG AGCCGCTCAG 
CCTGTCTCAT CCACCGCAGA ACAGCAGGAA TCGTCCAGTT CAGATCAGAC AAATGATTCG 
GAAGGGGCTT CATGGCCTGC TCCCTTCGAA ATGCCTTCTT CAGTTTCTGA AGATCCCAGT 
GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA 
GCCCCCAAAG AACAAAACCT GATTACTTTA TTCAATGACG GGGAGCACAG TCAGGGTCTT 
AAAAATGATT TTGTTCGGAA TATTCTATGG ACATTTGAAG ATATCCATAT GTTGGTCGGC 
TCCAACATGC CCATATTTGG AGGAGGCAGA TACCCAGCAG TCAGCTTACG TCTCAGGGAT 
AACAACAAAC CAATTAATGT GCTAACTGGA ATTGACTATT GGTTGGACAA CTTGATATGC 
AATGTGCCAG AGCTTGTGAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA ATATGAAATG 
ATAAAGACAG AAGAAATTCC CAATTTGGAA AATTCTAATT TTTCTACTAA AGTCATAAAA 
GACATTGCAC AGAATATTTT ATCATTTTTG AAATCTAATT GTACCAAAGA AGGACATACC 
TATTGGCTCT TTAAAGCAAG TGGCAGCGAT ATAGTGAAGC TCTATGACCT CACTACTCTT 
TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCGGTAGC CATTCTCTTG 
TACAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTATGGAACT 
ATTAGAACAT TGCTTCTTAA TTGTCTGAAA TTACTGGACA AAAGTAGGCA TCCTCAAATT 
ATTGCTTCCG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAGGAA 
GAAAATTCAG AATCTCCTTT AAATGAGAAT TCTGATGAAA GTTATAGTGA AGAGGAGGAA 
GAGATGCCCG ACAGTGATGA AAATGGATCC TATAGCACCA GCTCTGATCC ATCAGATGAT 
AGCAAAGCAG TAGCTATAAT CAAGTCTGTT GGAGAACTAT CAGTACCAGA AAAATACAAA 
TCTATTCATC AAATCAGACC CAGTTGTGCA TTTCCAGTTT GCCATGACAC AGAAGAGCGC 
TGTAGACTTG TGCTTAGCTA TGTTCTAGAG GGTTTAAAAT CTGTCGATAG CAGCATCAAA 
AAAGAAAGCG ACCTTCCAGC AGCTGACCCC AGCACTCCAA TCCCGTTAAA ATATGAAGAT 
GAATCCTCAA GAGGGGGTCC CGAGGGGCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA 
ATGGGCTCCC TTCAGAAGGG CAATTATTCC AGTCAATCTG GAATGATCCC TGGCTCTTGG 
CAACATAAAA TGAAACTTCA GCTGATTCTC AAGTCATCAA AGGCCTATTA TGTTTTGTCC 
GATGCTGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG 
CAAAGCCATG ATACTTATTG CTGCCTCTGC ACCAATATGC TTTCCGAAGT GCTGTTGTTT 
CTCTCTCAAT ATTTGACACT TTGTGGTGAT ATCCAACTAA TGCTGGCCCA GAATGCAAAT 
AATAGAGCAG CACACCTTGA AGAGTTTCAT TACCAAACAA AAGAAGACCA GGAGATCCTG 
CATAGCCTTC ACAGAGAGTC CAGTTGCCAA GGATTTGCAT GGGCAACTGA TTTGTCTACA 
GACTTAGAAA GTCAACTCTC TGTTAGTTGT AAATGTTATG AGGCTGCTAA TGAAATCTTG 
CAGTTTAGTG ACTTGAAAAG CCAAAATCCA GAACACTATG TACAAGTATT AAAGAGAATG 
GGTAACATTA GAAATGAAAT TGGTGTGTTT TACATGAATC AGGCTGCTGC ATTACAGAGT 
GAGAGACTAG TGAGCAAATC TGTGTCTGCT GCCGAGCAAC AGTTGTGGAA AAAAAGCTTT 
TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAATCAATTG AGGATGCCAC CAATGCCGCC 
CTTTTATTAT GTAACACGGG AAGGCTCATG CGGATTTGTG CGCAGGCCCA CTGTGGTGCA 
GGGGATGAAC TGAAACGTGA ATTTTCACCA GAAGAAGGCT TGTATTATAA TAAGGCTATT 
GATTACTATT TGAAAGCGCT AAGGTCATTG GGAACACGAG ACATACACCC AGCTGTTTGG 
GATTCAGTGA ACTGGGAATT GTCCACTACT TACTTTACTA TGGCAACTCT ACAGCAAGAT 
TATGCTCCGT TATCTAGAAA AGCTCAGGAG CAGATTGAGA AAGAAGTCAG TGAGGCCATG 
ATGAAGTCCC TAAAATACTG CGATGTGGAT TCAGTGTCTG CTCGACAGCC CCTTTGTCAG 
TATCGAGCTG CAACCATCCA TCACAGGCTG GCCTCCATGT ACCACAGCTG TCTGAGGAAT 
CAGGTTGGTG ATGAACACCT TAGGAAACAA CACCGGGTGC TGGCAGATCT TCATTACAGC 
AAGGCCGCAA AGCTGTTTCA GCTGCTGAAA GATGCTCCCT GCGAACTGCT TAGAGTACAG 
CTAGAGAGAG TAGCATTTGC TGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGAAAG 
TTGAAAACAC TATCTGGGGC TCTTGATATA ATGGTGAGAA CTGAGCACGC ATTCCAGCTT 
ATCCAGAAGG AGCTTATAGA AGAATTTGGC CAGCCTAAGA GTGGTGACGC CGCTGCAGCT 
GCTGATGCTT CTCCTAGTCT CAATCGAGAA GAAGTGATGA AACTCCTCAG TATATTTGAG 
TCTCGGTTGT CATTTCTTCT CCTTCAGTCC ATTAAACTGC TATCTTCAAC TAAAAAGAAA 
ACAAGCAATA ACATCGAAGA TGACACAATT CTCAAAACCA ACAAGCACAT TTACTCCCAG 
CTTTTGAGAG CAACTGCAAA TAAAACCGCG ACTCTTCTGG AAAGAATCAA CGTTATCGTC 
CACCTGCTGG GCCAGCTTGC CGCCGGCAGT GCAGCGAGCA GCAATGCCGT TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189367.1
CDS	100..3816
Translation	MGDAKEAGAEGPPAGAAARGGLSLLSQGESEESSAQGSALFLGGNEVKSRAVVKYSSAPPRTAFARLEEKTDLKLPPANWLRESAKLGPAGTTILGNSKKSKPFSSFGMAYDFIDSVGNDVDVVSDSENIKKLLKIPYSKSHVSMAVHRIGRTLLLDELDIQELFMRSSQTGDWTWLKEFYQRLIDQKWQRKKKSKEHWYQKAILSKFLYYSINGDGAAQPVSSTAEQQESSSSDQTNDSEGASWPAPFEMPSSVSEDPSASSQGSEPLEPSYIVGHVASAPKEQNLITLFNDGEHSQGLKNDFVRNILWTFEDIHMLVGSNMPIFGGGRYPAVSLRLRDNNKPINVLTGIDYWLDNLICNVPELVMCFHVNGIVQKYEMIKTEEIPNLENSNFSTKVIKDIAQNILSFLKSNCTKEGHTYWLFKASGSDIVKLYDLTTLCEETEDKYQNPFTMPVAILLYKVACNMMMKKNQNKKHYGTIRTLLLNCLKLLDKSRHPQIIASANYMLSELFQLDEPKKEENSESPLNENSDESYSEEEEEMPDSDENGSYSTSSDPSDDSKAVAIIKSVGELSVPEKYKSIHQIRPSCAFPVCHDTEERCRLVLSYVLEGLKSVDSSIKKESDLPAADPSTPIPLKYEDESSRGGPEGLEKQMALFLDKMGSLQKGNYSSQSGMIPGSWQHKMKLQLILKSSKAYYVLSDAAMSLQKYGRALRYIKLALQSHDTYCCLCTNMLSEVLLFLSQYLTLCGDIQLMLAQNANNRAAHLEEFHYQTKEDQEILHSLHRESSCQGFAWATDLSTDLESQLSVSCKCYEAANEILQFSDLKSQNPEHYVQVLKRMGNIRNEIGVFYMNQAAALQSERLVSKSVSAAEQQLWKKSFSCFEKGIHNFESIEDATNAALLLCNTGRLMRICAQAHCGAGDELKREFSPEEGLYYNKAIDYYLKALRSLGTRDIHPAVWDSVNWELSTTYFTMATLQQDYAPLSRKAQEQIEKEVSEAMMKSLKYCDVDSVSARQPLCQYRAATIHHRLASMYHSCLRNQVGDEHLRKQHRVLADLHYSKAAKLFQLLKDAPCELLRVQLERVAFAEFQMTSQNSNVGKLKTLSGALDIMVRTEHAFQLIQKELIEEFGQPKSGDAAAAADASPSLNREEVMKLLSIFESRLSFLLLQSIKLLSSTKKKTSNNIEDDTILKTNKHIYSQLLRATANKTATLLERINVIVHLLGQLAAGSAASSNAVQ

Target ORF information:

RefSeq Version	NM_001202438.2
Organism	Homo sapiens(human)
Definition	Homo sapiens erythroid differentiation regulatory factor 1 (EDRF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202438.2

ORF Insert Sequence:

ATGGGGGATG CCAAGGAGGC CGGAGCCGAG GGTCCGCCGG CCGGGGCCGC CGCTCGAGGA 
GGGCTCAGCC TCCTGTCCCA GGGAGAATCC GAGGAATCTT CTGCACAGGG ATCAGCTTTA 
TTTCTTGGAG GCAATGAAGT GAAGAGCCGA GCTGTGGTGA AATACTCTTC TGCCCCTCCT 
CGAACAGCAT TTGCACGCCT TGAAGAGAAA ACAGACTTGA AACTCCCACC TGCCAACTGG 
TTACGAGAGA GTGCCAAACT AGGGCCAGCA GGAACTACCA TTCTTGGCAA CAGCAAGAAA 
AGCAAGCCAT TTTCAAGCTT TGGCATGGCA TATGACTTTA TTGATTCAGT GGGAAATGAT 
GTGGATGTTG TCTCTGACTC TGAAAACATA AAAAAACTCC TGAAAATTCC CTACAGCAAG 
TCGCACGTGA GCATGGCAGT ACACCGCATA GGAAGGACTC TCTTACTAGA TGAGCTAGAT 
ATTCAAGAAC TCTTTATGAG ATCGTCTCAG ACTGGTGACT GGACATGGTT GAAAGAGTTT 
TATCAAAGAC TCATTGATCA GAAGTGGCAG AGGAAGAAAA AGAGCAAAGA GCACTGGTAT 
CAAAAGGCAA TTCTTTCCAA GTTTTTATAT TACAGTATCA ATGGTGATGG AGCCGCTCAG 
CCTGTCTCAT CCACCGCAGA ACAGCAGGAA TCGTCCAGTT CAGATCAGAC AAATGATTCG 
GAAGGGGCTT CATGGCCTGC TCCCTTCGAA ATGCCTTCTT CAGTTTCTGA AGATCCCAGT 
GCTTCCAGTC AGGGAAGTGA GCCTCTTGAA CCCTCATACA TAGTGGGGCA TGTGGCCTCA 
GCCCCCAAAG AACAAAACCT GATTACTTTA TTCAATGACG GGGAGCACAG TCAGGGTCTT 
AAAAATGATT TTGTTCGGAA TATTCTATGG ACATTTGAAG ATATCCATAT GTTGGTCGGC 
TCCAACATGC CCATATTTGG AGGAGGCAGA TACCCAGCAG TCAGCTTACG TCTCAGGGAT 
AACAACAAAC CAATTAATGT GCTAACTGGA ATTGACTATT GGTTGGACAA CTTGATATGC 
AATGTGCCAG AGCTTGTGAT GTGTTTTCAT GTAAATGGAA TTGTACAGAA ATATGAAATG 
ATAAAGACAG AAGAAATTCC CAATTTGGAA AATTCTAATT TTTCTACTAA AGTCATAAAA 
GACATTGCAC AGAATATTTT ATCATTTTTG AAATCTAATT GTACCAAAGA AGGACATACC 
TATTGGCTCT TTAAAGCAAG TGGCAGCGAT ATAGTGAAGC TCTATGACCT CACTACTCTT 
TGTGAAGAAA CTGAAGACAA ATACCAAAAT CCATTCACAA TGCCGGTAGC CATTCTCTTG 
TACAAGGTTG CTTGCAACAT GATGATGAAG AAGAATCAAA ATAAGAAACA CTATGGAACT 
ATTAGAACAT TGCTTCTTAA TTGTCTGAAA TTACTGGACA AAAGTAGGCA TCCTCAAATT 
ATTGCTTCCG CCAATTACAT GCTTTCAGAA CTTTTTCAAT TGGATGAACC TAAAAAGGAA 
GAAAATTCAG AATCTCCTTT AAATGAGAAT TCTGATGAAA GTTATAGTGA AGAGGAGGAA 
GAGATGCCCG ACAGTGATGA AAATGGATCC TATAGCACCA GCTCTGATCC ATCAGATGAT 
AGCAAAGCAG TAGCTATAAT CAAGTCTGTT GGAGAACTAT CAGTACCAGA AAAATACAAA 
TCTATTCATC AAATCAGACC CAGTTGTGCA TTTCCAGTTT GCCATGACAC AGAAGAGCGC 
TGTAGACTTG TGCTTAGCTA TGTTCTAGAG GGTTTAAAAT CTGTCGATAG CAGCATCAAA 
AAAGAAAGCG ACCTTCCAGC AGCTGACCCC AGCACTCCAA TCCCGTTAAA ATATGAAGAT 
GAATCCTCAA GAGGGGGTCC CGAGGGGCTA GAGAAGCAGA TGGCCTTGTT TTTGGACAAA 
ATGGGCTCCC TTCAGAAGGG CAATTATTCC AGTCAATCTG GAATGATCCC TGGCTCTTGG 
CAACATAAAA TGAAACTTCA GCTGATTCTC AAGTCATCAA AGGCCTATTA TGTTTTGTCC 
GATGCTGCCA TGAGTCTTCA GAAATACGGA AGAGCATTAC GATACATTAA ATTAGCTTTG 
CAAAGCCATG ATACTTATTG CTGCCTCTGC ACCAATATGC TTTCCGAAGT GCTGTTGTTT 
CTCTCTCAAT ATTTGACACT TTGTGGTGAT ATCCAACTAA TGCTGGCCCA GAATGCAAAT 
AATAGAGCAG CACACCTTGA AGAGTTTCAT TACCAAACAA AAGAAGACCA GGAGATCCTG 
CATAGCCTTC ACAGAGAGTC CAGTTGCCAA GGATTTGCAT GGGCAACTGA TTTGTCTACA 
GACTTAGAAA GTCAACTCTC TGTTAGTTGT AAATGTTATG AGGCTGCTAA TGAAATCTTG 
CAGTTTAGTG ACTTGAAAAG CCAAAATCCA GAACACTATG TACAAGTATT AAAGAGAATG 
GGTAACATTA GAAATGAAAT TGGTGTGTTT TACATGAATC AGGCTGCTGC ATTACAGAGT 
GAGAGACTAG TGAGCAAATC TGTGTCTGCT GCCGAGCAAC AGTTGTGGAA AAAAAGCTTT 
TCTTGTTTTG AAAAGGGAAT TCACAACTTT GAATCAATTG AGGATGCCAC CAATGCCGCC 
CTTTTATTAT GTAACACGGG AAGGCTCATG CGGATTTGTG CGCAGGCCCA CTGTGGTGCA 
GGGGATGAAC TGAAACGTGA ATTTTCACCA GAAGAAGGCT TGTATTATAA TAAGGCTATT 
GATTACTATT TGAAAGCGCT AAGGTCATTG GGAACACGAG ACATACACCC AGCTGTTTGG 
GATTCAGTGA ACTGGGAATT GTCCACTACT TACTTTACTA TGGCAACTCT ACAGCAAGAT 
TATGCTCCGT TATCTAGAAA AGCTCAGGAG CAGATTGAGA AAGAAGTCAG TGAGGCCATG 
ATGAAGTCCC TAAAATACTG CGATGTGGAT TCAGTGTCTG CTCGACAGCC CCTTTGTCAG 
TATCGAGCTG CAACCATCCA TCACAGGCTG GCCTCCATGT ACCACAGCTG TCTGAGGAAT 
CAGGTTGGTG ATGAACACCT TAGGAAACAA CACCGGGTGC TGGCAGATCT TCATTACAGC 
AAGGCCGCAA AGCTGTTTCA GCTGCTGAAA GATGCTCCCT GCGAACTGCT TAGAGTACAG 
CTAGAGAGAG TAGCATTTGC TGAATTTCAG ATGACCAGTC AGAATAGCAA TGTTGGAAAG 
TTGAAAACAC TATCTGGGGC TCTTGATATA ATGGTGAGAA CTGAGCACGC ATTCCAGCTT 
ATCCAGAAGG AGCTTATAGA AGAATTTGGC CAGCCTAAGA GTGGTGACGC CGCTGCAGCT 
GCTGATGCTT CTCCTAGTCT CAATCGAGAA GAAGTGATGA AACTCCTCAG TATATTTGAG 
TCTCGGTTGT CATTTCTTCT CCTTCAGTCC ATTAAACTGC TATCTTCAAC TAAAAAGAAA 
ACAAGCAATA ACATCGAAGA TGACACAATT CTCAAAACCA ACAAGCACAT TTACTCCCAG 
CTTTTGAGAG CAACTGCAAA TAAAACCGCG ACTCTTCTGG AAAGAATCAA CGTTATCGTC 
CACCTGCTGG GCCAGCTTGC CGCCGGCAGT GCAGCGAGCA GCAATGCCGT TCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel erythroid differentiation related gene EDRF1 upregulating globin gene expression in HEL cells.
Chinese medical journal115(11)1701-5(2002 Nov)
Wang D,Li Y,Shen B

Antisense EDRF1 gene inhibited GATA-1 transcription factor DNA-binding activity in K562 cells.
Science in China. Series C, Life sciences45(3)289-97(2002 Jun)
Wang D,Ma S,Wang S,Shen B

Initial function analysis of a novel erythroid differentiation related gene EDRF1.
Science in China. Series C, Life sciences44(5)489-96(2001 Oct)
Wang D,Li Y,Shen B

cDNA cloning and function analysis of two novel erythroid differentiation related genes.
Science in China. Series C, Life sciences44(1)99-105(2001 Feb)
Wang X,Wang D,Chen X,Hu M,Wang J,Li Y,Guo N,Shen B

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オンライン注文

EDRF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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