TSKU cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSKU
Entrez Gene ID	25987
Full Name	tsukushi, small leucine rich proteoglycan
Synonyms	E2IG4,LRRC54,TSK
General protein information	Preferred Names tsukushi, small leucine rich proteoglycan Names tsukushin E2-induced gene 4 protein leucine rich repeat containing 54 leucine-rich repeat-containing protein 54 tsukushi homolog tsukushi small leucine rich proteoglycan homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.5
Disorder MIM:	608015

mRNA and Protein(s)

mRNA	Protein	Name
NM_015516.3	NP_056331.2	tsukushin isoform b precursor
NM_001318479.1	NP_001305408.1	tsukushin isoform b precursor
NM_001318477.1	NP_001305406.1	tsukushin isoform a precursor
NM_015516.4	NP_056331.2	tsukushin isoform b precursor
NM_001318478.1	NP_001305407.1	tsukushin isoform b precursor
NM_001258210.1	NP_001245139.1	tsukushin isoform b precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tsku	NP_001009965.1
Gallus gallus (chicken)	TSKU	NP_001005346.1
Mus musculus (house mouse)	Tsku	NP_001162011.1
Canis lupus familiaris (dog)	TSKU	XP_005633522.1
Homo sapiens (human)	TSKU	NP_001245139.1
Xenopus tropicalis (tropical clawed frog)	tsku	NP_001011330.1
Pan troglodytes (chimpanzee)	TSKU	XP_508654.3
	TSKU	XP_002799755.1
Danio rerio (zebrafish)	tsku	NP_956027.1

Gene Ontology
Bibliography

Related articles in PubMed

Tsukushi controls the hair cycle by regulating TGF-?1 signaling.
Niimori D, Kawano R, Felemban A, Niimori-Kita K, Tanaka H, Ihn H, Ohta K
Developmental biology372(1)81-7(2012 Dec)

Stromal expression of decorin, Semaphorin6D, SPARC, Sprouty1 and Tsukushi in developing prostate and decreased levels of decorin in prostate cancer.
Henke A, Grace OC, Ashley GR, Stewart GD, Riddick AC, Yeun H, O'Donnell M, Anderson RA, Thomson AA
PloS one7(8)e42516(2012)

Comparative analysis of gene regulation by the transcription factor PPARalpha between mouse and human.
Rakhshandehroo M, Hooiveld G, M?ller M, Kersten S
PloS one4(8)e6796(2009 Aug)

Functional proteomics mapping of a human signaling pathway.
Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM
Genome research14(7)1324-32(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TSK controls the hair cycle by regulating TGF-beta1 signaling.
Title: Tsukushi controls the hair cycle by regulating TGF-?1 signaling.

The spatio-temporal expression of Decorin, Semaphorin6D, SPARC, Spry-1 and Tsukushi in developing prostate is described and observed similar mesenchymal expression patterns in rat and human.
Title: Stromal expression of decorin, Semaphorin6D, SPARC, Sprouty1 and Tsukushi in developing prostate and decreased levels of decorin in prostate cancer.

The following TSKU gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSKU cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03186	NM_015516.4 Latest version!	Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu03186	NM_001318479.1 Latest version!	Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu03186	NM_001318478.1 Latest version!	Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu03186	NM_001258210.1 Latest version!	Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu45266	NM_001318477.1 Latest version!	Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu03186	NM_015516.3	Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSKU ( NM_015516.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056331.2 Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03186
Clone ID Related Accession (Same CDS sequence)	NM_015516.3 , NM_001318479.1 , NM_015516.4 , NM_001318478.1 , NM_001258210.1
Accession Version	NM_015516.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	tsukushin isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP003119.2, AK091475.1 and BC020975.1. On Nov 22, 2004 this sequence version replaced NM_015516.2. Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 1, 2, 4, and 5 all encode isoform b. ##Evidence-Data-START## Transcript exon combination :: BC020975.1, AK075476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGTGGC CCCTGCTGCT GCTGCTGGCC GTGAGTGGGG CCCAGACAAC CCGGCCATGC 
TTCCCCGGGT GCCAATGCGA GGTGGAGACC TTCGGCCTTT TCGACAGCTT CAGCCTGACT 
CGGGTGGATT GTAGCGGCCT GGGCCCCCAC ATCATGCCGG TGCCCATCCC TCTGGACACA 
GCCCACTTGG ACCTGTCCTC CAACCGGCTG GAGATGGTGA ATGAGTCGGT GTTGGCGGGG 
CCGGGCTACA CGACGTTGGC TGGCCTGGAT CTCAGCCACA ACCTGCTCAC CAGCATCTCA 
CCCACTGCCT TCTCCCGCCT TCGCTACCTG GAGTCGCTTG ACCTCAGCCA CAATGGCCTG 
ACAGCCCTGC CAGCCGAGAG CTTCACCAGC TCACCCCTGA GCGACGTGAA CCTTAGCCAC 
AACCAGCTCC GGGAGGTCTC AGTGTCTGCC TTCACGACGC ACAGTCAGGG CCGGGCACTA 
CACGTGGACC TCTCCCACAA CCTCATTCAC CGCCTCGTGC CCCACCCCAC GAGGGCCGGC 
CTGCCTGCGC CCACCATTCA GAGCCTGAAC CTGGCCTGGA ACCGGCTCCA TGCCGTGCCC 
AACCTCCGAG ACTTGCCCCT GCGCTACCTG AGCCTGGATG GGAACCCTCT AGCTGTCATT 
GGTCCGGGTG CCTTCGCGGG GCTGGGAGGC CTTACACACC TGTCTCTGGC CAGCCTGCAG 
AGGCTCCCTG AGCTGGCGCC CAGTGGCTTC CGTGAGCTAC CGGGCCTGCA GGTCCTGGAC 
CTGTCGGGCA ACCCCAAGCT TAACTGGGCA GGAGCTGAGG TGTTTTCAGG CCTGAGCTCC 
CTGCAGGAGC TGGACCTTTC GGGCACCAAC CTGGTGCCCC TGCCTGAGGC GCTGCTCCTC 
CACCTCCCGG CACTGCAGAG CGTCAGCGTG GGCCAGGATG TGCGGTGCCG GCGCCTGGTG 
CGGGAGGGCA CCTACCCCCG GAGGCCTGGC TCCAGCCCCA AGGTGGCCCT GCACTGCGTA 
GACACCCGGG ATTCTGCTGC CAGGGGCCCC ACCATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056331.2
CDS	173..1234
Translation	MPWPLLLLLAVSGAQTTRPCFPGCQCEVETFGLFDSFSLTRVDCSGLGPHIMPVPIPLDTAHLDLSSNRLEMVNESVLAGPGYTTLAGLDLSHNLLTSISPTAFSRLRYLESLDLSHNGLTALPAESFTSSPLSDVNLSHNQLREVSVSAFTTHSQGRALHVDLSHNLIHRLVPHPTRAGLPAPTIQSLNLAWNRLHAVPNLRDLPLRYLSLDGNPLAVIGPGAFAGLGGLTHLSLASLQRLPELAPSGFRELPGLQVLDLSGNPKLNWAGAEVFSGLSSLQELDLSGTNLVPLPEALLLHLPALQSVSVGQDVRCRRLVREGTYPRRPGSSPKVALHCVDTRDSAARGPTIL

Target ORF information:

RefSeq Version	NM_015516.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015516.3

ORF Insert Sequence:

ATGCCGTGGC CCCTGCTGCT GCTGCTGGCC GTGAGTGGGG CCCAGACAAC CCGGCCATGC 
TTCCCCGGGT GCCAATGCGA GGTGGAGACC TTCGGCCTTT TCGACAGCTT CAGCCTGACT 
CGGGTGGATT GTAGCGGCCT GGGCCCCCAC ATCATGCCGG TGCCCATCCC TCTGGACACA 
GCCCACTTGG ACCTGTCCTC CAACCGGCTG GAGATGGTGA ATGAGTCGGT GTTGGCGGGG 
CCGGGCTACA CGACGTTGGC TGGCCTGGAT CTCAGCCACA ACCTGCTCAC CAGCATCTCA 
CCCACTGCCT TCTCCCGCCT TCGCTACCTG GAGTCGCTTG ACCTCAGCCA CAATGGCCTG 
ACAGCCCTGC CAGCCGAGAG CTTCACCAGC TCACCCCTGA GCGACGTGAA CCTTAGCCAC 
AACCAGCTCC GGGAGGTCTC AGTGTCTGCC TTCACGACGC ACAGTCAGGG CCGGGCACTA 
CACGTGGACC TCTCCCACAA CCTCATTCAC CGCCTCGTGC CCCACCCCAC GAGGGCCGGC 
CTGCCTGCGC CCACCATTCA GAGCCTGAAC CTGGCCTGGA ACCGGCTCCA TGCCGTGCCC 
AACCTCCGAG ACTTGCCCCT GCGCTACCTG AGCCTGGATG GGAACCCTCT AGCTGTCATT 
GGTCCGGGTG CCTTCGCGGG GCTGGGAGGC CTTACACACC TGTCTCTGGC CAGCCTGCAG 
AGGCTCCCTG AGCTGGCGCC CAGTGGCTTC CGTGAGCTAC CGGGCCTGCA GGTCCTGGAC 
CTGTCGGGCA ACCCCAAGCT TAACTGGGCA GGAGCTGAGG TGTTTTCAGG CCTGAGCTCC 
CTGCAGGAGC TGGACCTTTC GGGCACCAAC CTGGTGCCCC TGCCTGAGGC GCTGCTCCTC 
CACCTCCCGG CACTGCAGAG CGTCAGCGTG GGCCAGGATG TGCGGTGCCG GCGCCTGGTG 
CGGGAGGGCA CCTACCCCCG GAGGCCTGGC TCCAGCCCCA AGGTGGCCCT GCACTGCGTA 
GACACCCGGG ATTCTGCTGC CAGGGGCCCC ACCATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSKU ( NM_001318479.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305408.1 Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu03186
Clone ID Related Accession (Same CDS sequence)	NM_015516.3 , NM_001318479.1 , NM_015516.4 , NM_001318478.1 , NM_001258210.1
Accession Version	NM_001318479.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Homo sapiens(human)
Product	tsukushin isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA879213.1 and BC020975.1. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 1, 2, 4, and 5 all encode isoform b. ##Evidence-Data-START## Transcript exon combination :: DA879213.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGTGGC CCCTGCTGCT GCTGCTGGCC GTGAGTGGGG CCCAGACAAC CCGGCCATGC 
TTCCCCGGGT GCCAATGCGA GGTGGAGACC TTCGGCCTTT TCGACAGCTT CAGCCTGACT 
CGGGTGGATT GTAGCGGCCT GGGCCCCCAC ATCATGCCGG TGCCCATCCC TCTGGACACA 
GCCCACTTGG ACCTGTCCTC CAACCGGCTG GAGATGGTGA ATGAGTCGGT GTTGGCGGGG 
CCGGGCTACA CGACGTTGGC TGGCCTGGAT CTCAGCCACA ACCTGCTCAC CAGCATCTCA 
CCCACTGCCT TCTCCCGCCT TCGCTACCTG GAGTCGCTTG ACCTCAGCCA CAATGGCCTG 
ACAGCCCTGC CAGCCGAGAG CTTCACCAGC TCACCCCTGA GCGACGTGAA CCTTAGCCAC 
AACCAGCTCC GGGAGGTCTC AGTGTCTGCC TTCACGACGC ACAGTCAGGG CCGGGCACTA 
CACGTGGACC TCTCCCACAA CCTCATTCAC CGCCTCGTGC CCCACCCCAC GAGGGCCGGC 
CTGCCTGCGC CCACCATTCA GAGCCTGAAC CTGGCCTGGA ACCGGCTCCA TGCCGTGCCC 
AACCTCCGAG ACTTGCCCCT GCGCTACCTG AGCCTGGATG GGAACCCTCT AGCTGTCATT 
GGTCCGGGTG CCTTCGCGGG GCTGGGAGGC CTTACACACC TGTCTCTGGC CAGCCTGCAG 
AGGCTCCCTG AGCTGGCGCC CAGTGGCTTC CGTGAGCTAC CGGGCCTGCA GGTCCTGGAC 
CTGTCGGGCA ACCCCAAGCT TAACTGGGCA GGAGCTGAGG TGTTTTCAGG CCTGAGCTCC 
CTGCAGGAGC TGGACCTTTC GGGCACCAAC CTGGTGCCCC TGCCTGAGGC GCTGCTCCTC 
CACCTCCCGG CACTGCAGAG CGTCAGCGTG GGCCAGGATG TGCGGTGCCG GCGCCTGGTG 
CGGGAGGGCA CCTACCCCCG GAGGCCTGGC TCCAGCCCCA AGGTGGCCCT GCACTGCGTA 
GACACCCGGG ATTCTGCTGC CAGGGGCCCC ACCATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305408.1
CDS	38..1099
Translation	MPWPLLLLLAVSGAQTTRPCFPGCQCEVETFGLFDSFSLTRVDCSGLGPHIMPVPIPLDTAHLDLSSNRLEMVNESVLAGPGYTTLAGLDLSHNLLTSISPTAFSRLRYLESLDLSHNGLTALPAESFTSSPLSDVNLSHNQLREVSVSAFTTHSQGRALHVDLSHNLIHRLVPHPTRAGLPAPTIQSLNLAWNRLHAVPNLRDLPLRYLSLDGNPLAVIGPGAFAGLGGLTHLSLASLQRLPELAPSGFRELPGLQVLDLSGNPKLNWAGAEVFSGLSSLQELDLSGTNLVPLPEALLLHLPALQSVSVGQDVRCRRLVREGTYPRRPGSSPKVALHCVDTRDSAARGPTIL

Target ORF information:

RefSeq Version	NM_001318479.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318479.1

ORF Insert Sequence:

ATGCCGTGGC CCCTGCTGCT GCTGCTGGCC GTGAGTGGGG CCCAGACAAC CCGGCCATGC 
TTCCCCGGGT GCCAATGCGA GGTGGAGACC TTCGGCCTTT TCGACAGCTT CAGCCTGACT 
CGGGTGGATT GTAGCGGCCT GGGCCCCCAC ATCATGCCGG TGCCCATCCC TCTGGACACA 
GCCCACTTGG ACCTGTCCTC CAACCGGCTG GAGATGGTGA ATGAGTCGGT GTTGGCGGGG 
CCGGGCTACA CGACGTTGGC TGGCCTGGAT CTCAGCCACA ACCTGCTCAC CAGCATCTCA 
CCCACTGCCT TCTCCCGCCT TCGCTACCTG GAGTCGCTTG ACCTCAGCCA CAATGGCCTG 
ACAGCCCTGC CAGCCGAGAG CTTCACCAGC TCACCCCTGA GCGACGTGAA CCTTAGCCAC 
AACCAGCTCC GGGAGGTCTC AGTGTCTGCC TTCACGACGC ACAGTCAGGG CCGGGCACTA 
CACGTGGACC TCTCCCACAA CCTCATTCAC CGCCTCGTGC CCCACCCCAC GAGGGCCGGC 
CTGCCTGCGC CCACCATTCA GAGCCTGAAC CTGGCCTGGA ACCGGCTCCA TGCCGTGCCC 
AACCTCCGAG ACTTGCCCCT GCGCTACCTG AGCCTGGATG GGAACCCTCT AGCTGTCATT 
GGTCCGGGTG CCTTCGCGGG GCTGGGAGGC CTTACACACC TGTCTCTGGC CAGCCTGCAG 
AGGCTCCCTG AGCTGGCGCC CAGTGGCTTC CGTGAGCTAC CGGGCCTGCA GGTCCTGGAC 
CTGTCGGGCA ACCCCAAGCT TAACTGGGCA GGAGCTGAGG TGTTTTCAGG CCTGAGCTCC 
CTGCAGGAGC TGGACCTTTC GGGCACCAAC CTGGTGCCCC TGCCTGAGGC GCTGCTCCTC 
CACCTCCCGG CACTGCAGAG CGTCAGCGTG GGCCAGGATG TGCGGTGCCG GCGCCTGGTG 
CGGGAGGGCA CCTACCCCCG GAGGCCTGGC TCCAGCCCCA AGGTGGCCCT GCACTGCGTA 
GACACCCGGG ATTCTGCTGC CAGGGGCCCC ACCATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSKU ( NM_015516.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056331.2 Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03186
Clone ID Related Accession (Same CDS sequence)	NM_015516.3 , NM_001318479.1 , NM_015516.4 , NM_001318478.1 , NM_001258210.1
Accession Version	NM_015516.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Homo sapiens(human)
Product	tsukushin isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK091475.1 and BC020975.1. On Nov 22, 2018 this sequence version replaced NM_015516.3. Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 1, 2, 4, and 5 all encode isoform b. ##Evidence-Data-START## Transcript exon combination :: AY358317.1, AK075476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000333090.5/ ENSP00000332668.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGTGGC CCCTGCTGCT GCTGCTGGCC GTGAGTGGGG CCCAGACAAC CCGGCCATGC 
TTCCCCGGGT GCCAATGCGA GGTGGAGACC TTCGGCCTTT TCGACAGCTT CAGCCTGACT 
CGGGTGGATT GTAGCGGCCT GGGCCCCCAC ATCATGCCGG TGCCCATCCC TCTGGACACA 
GCCCACTTGG ACCTGTCCTC CAACCGGCTG GAGATGGTGA ATGAGTCGGT GTTGGCGGGG 
CCGGGCTACA CGACGTTGGC TGGCCTGGAT CTCAGCCACA ACCTGCTCAC CAGCATCTCA 
CCCACTGCCT TCTCCCGCCT TCGCTACCTG GAGTCGCTTG ACCTCAGCCA CAATGGCCTG 
ACAGCCCTGC CAGCCGAGAG CTTCACCAGC TCACCCCTGA GCGACGTGAA CCTTAGCCAC 
AACCAGCTCC GGGAGGTCTC AGTGTCTGCC TTCACGACGC ACAGTCAGGG CCGGGCACTA 
CACGTGGACC TCTCCCACAA CCTCATTCAC CGCCTCGTGC CCCACCCCAC GAGGGCCGGC 
CTGCCTGCGC CCACCATTCA GAGCCTGAAC CTGGCCTGGA ACCGGCTCCA TGCCGTGCCC 
AACCTCCGAG ACTTGCCCCT GCGCTACCTG AGCCTGGATG GGAACCCTCT AGCTGTCATT 
GGTCCGGGTG CCTTCGCGGG GCTGGGAGGC CTTACACACC TGTCTCTGGC CAGCCTGCAG 
AGGCTCCCTG AGCTGGCGCC CAGTGGCTTC CGTGAGCTAC CGGGCCTGCA GGTCCTGGAC 
CTGTCGGGCA ACCCCAAGCT TAACTGGGCA GGAGCTGAGG TGTTTTCAGG CCTGAGCTCC 
CTGCAGGAGC TGGACCTTTC GGGCACCAAC CTGGTGCCCC TGCCTGAGGC GCTGCTCCTC 
CACCTCCCGG CACTGCAGAG CGTCAGCGTG GGCCAGGATG TGCGGTGCCG GCGCCTGGTG 
CGGGAGGGCA CCTACCCCCG GAGGCCTGGC TCCAGCCCCA AGGTGGCCCT GCACTGCGTA 
GACACCCGGG ATTCTGCTGC CAGGGGCCCC ACCATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056331.2
CDS	58..1119
Translation	MPWPLLLLLAVSGAQTTRPCFPGCQCEVETFGLFDSFSLTRVDCSGLGPHIMPVPIPLDTAHLDLSSNRLEMVNESVLAGPGYTTLAGLDLSHNLLTSISPTAFSRLRYLESLDLSHNGLTALPAESFTSSPLSDVNLSHNQLREVSVSAFTTHSQGRALHVDLSHNLIHRLVPHPTRAGLPAPTIQSLNLAWNRLHAVPNLRDLPLRYLSLDGNPLAVIGPGAFAGLGGLTHLSLASLQRLPELAPSGFRELPGLQVLDLSGNPKLNWAGAEVFSGLSSLQELDLSGTNLVPLPEALLLHLPALQSVSVGQDVRCRRLVREGTYPRRPGSSPKVALHCVDTRDSAARGPTIL

Target ORF information:

RefSeq Version	NM_015516.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015516.4

ORF Insert Sequence:

ATGCCGTGGC CCCTGCTGCT GCTGCTGGCC GTGAGTGGGG CCCAGACAAC CCGGCCATGC 
TTCCCCGGGT GCCAATGCGA GGTGGAGACC TTCGGCCTTT TCGACAGCTT CAGCCTGACT 
CGGGTGGATT GTAGCGGCCT GGGCCCCCAC ATCATGCCGG TGCCCATCCC TCTGGACACA 
GCCCACTTGG ACCTGTCCTC CAACCGGCTG GAGATGGTGA ATGAGTCGGT GTTGGCGGGG 
CCGGGCTACA CGACGTTGGC TGGCCTGGAT CTCAGCCACA ACCTGCTCAC CAGCATCTCA 
CCCACTGCCT TCTCCCGCCT TCGCTACCTG GAGTCGCTTG ACCTCAGCCA CAATGGCCTG 
ACAGCCCTGC CAGCCGAGAG CTTCACCAGC TCACCCCTGA GCGACGTGAA CCTTAGCCAC 
AACCAGCTCC GGGAGGTCTC AGTGTCTGCC TTCACGACGC ACAGTCAGGG CCGGGCACTA 
CACGTGGACC TCTCCCACAA CCTCATTCAC CGCCTCGTGC CCCACCCCAC GAGGGCCGGC 
CTGCCTGCGC CCACCATTCA GAGCCTGAAC CTGGCCTGGA ACCGGCTCCA TGCCGTGCCC 
AACCTCCGAG ACTTGCCCCT GCGCTACCTG AGCCTGGATG GGAACCCTCT AGCTGTCATT 
GGTCCGGGTG CCTTCGCGGG GCTGGGAGGC CTTACACACC TGTCTCTGGC CAGCCTGCAG 
AGGCTCCCTG AGCTGGCGCC CAGTGGCTTC CGTGAGCTAC CGGGCCTGCA GGTCCTGGAC 
CTGTCGGGCA ACCCCAAGCT TAACTGGGCA GGAGCTGAGG TGTTTTCAGG CCTGAGCTCC 
CTGCAGGAGC TGGACCTTTC GGGCACCAAC CTGGTGCCCC TGCCTGAGGC GCTGCTCCTC 
CACCTCCCGG CACTGCAGAG CGTCAGCGTG GGCCAGGATG TGCGGTGCCG GCGCCTGGTG 
CGGGAGGGCA CCTACCCCCG GAGGCCTGGC TCCAGCCCCA AGGTGGCCCT GCACTGCGTA 
GACACCCGGG ATTCTGCTGC CAGGGGCCCC ACCATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSKU ( NM_001318478.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305407.1 Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu03186
Clone ID Related Accession (Same CDS sequence)	NM_015516.3 , NM_001318479.1 , NM_015516.4 , NM_001318478.1 , NM_001258210.1
Accession Version	NM_001318478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Homo sapiens(human)
Product	tsukushin isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA623614.1 and BC020975.1. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 1, 2, 4, and 5 all encode isoform b. ##Evidence-Data-START## Transcript exon combination :: DA623614.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGTGGC CCCTGCTGCT GCTGCTGGCC GTGAGTGGGG CCCAGACAAC CCGGCCATGC 
TTCCCCGGGT GCCAATGCGA GGTGGAGACC TTCGGCCTTT TCGACAGCTT CAGCCTGACT 
CGGGTGGATT GTAGCGGCCT GGGCCCCCAC ATCATGCCGG TGCCCATCCC TCTGGACACA 
GCCCACTTGG ACCTGTCCTC CAACCGGCTG GAGATGGTGA ATGAGTCGGT GTTGGCGGGG 
CCGGGCTACA CGACGTTGGC TGGCCTGGAT CTCAGCCACA ACCTGCTCAC CAGCATCTCA 
CCCACTGCCT TCTCCCGCCT TCGCTACCTG GAGTCGCTTG ACCTCAGCCA CAATGGCCTG 
ACAGCCCTGC CAGCCGAGAG CTTCACCAGC TCACCCCTGA GCGACGTGAA CCTTAGCCAC 
AACCAGCTCC GGGAGGTCTC AGTGTCTGCC TTCACGACGC ACAGTCAGGG CCGGGCACTA 
CACGTGGACC TCTCCCACAA CCTCATTCAC CGCCTCGTGC CCCACCCCAC GAGGGCCGGC 
CTGCCTGCGC CCACCATTCA GAGCCTGAAC CTGGCCTGGA ACCGGCTCCA TGCCGTGCCC 
AACCTCCGAG ACTTGCCCCT GCGCTACCTG AGCCTGGATG GGAACCCTCT AGCTGTCATT 
GGTCCGGGTG CCTTCGCGGG GCTGGGAGGC CTTACACACC TGTCTCTGGC CAGCCTGCAG 
AGGCTCCCTG AGCTGGCGCC CAGTGGCTTC CGTGAGCTAC CGGGCCTGCA GGTCCTGGAC 
CTGTCGGGCA ACCCCAAGCT TAACTGGGCA GGAGCTGAGG TGTTTTCAGG CCTGAGCTCC 
CTGCAGGAGC TGGACCTTTC GGGCACCAAC CTGGTGCCCC TGCCTGAGGC GCTGCTCCTC 
CACCTCCCGG CACTGCAGAG CGTCAGCGTG GGCCAGGATG TGCGGTGCCG GCGCCTGGTG 
CGGGAGGGCA CCTACCCCCG GAGGCCTGGC TCCAGCCCCA AGGTGGCCCT GCACTGCGTA 
GACACCCGGG ATTCTGCTGC CAGGGGCCCC ACCATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305407.1
CDS	227..1288
Translation	MPWPLLLLLAVSGAQTTRPCFPGCQCEVETFGLFDSFSLTRVDCSGLGPHIMPVPIPLDTAHLDLSSNRLEMVNESVLAGPGYTTLAGLDLSHNLLTSISPTAFSRLRYLESLDLSHNGLTALPAESFTSSPLSDVNLSHNQLREVSVSAFTTHSQGRALHVDLSHNLIHRLVPHPTRAGLPAPTIQSLNLAWNRLHAVPNLRDLPLRYLSLDGNPLAVIGPGAFAGLGGLTHLSLASLQRLPELAPSGFRELPGLQVLDLSGNPKLNWAGAEVFSGLSSLQELDLSGTNLVPLPEALLLHLPALQSVSVGQDVRCRRLVREGTYPRRPGSSPKVALHCVDTRDSAARGPTIL

Target ORF information:

RefSeq Version	NM_001318478.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318478.1

ORF Insert Sequence:

ATGCCGTGGC CCCTGCTGCT GCTGCTGGCC GTGAGTGGGG CCCAGACAAC CCGGCCATGC 
TTCCCCGGGT GCCAATGCGA GGTGGAGACC TTCGGCCTTT TCGACAGCTT CAGCCTGACT 
CGGGTGGATT GTAGCGGCCT GGGCCCCCAC ATCATGCCGG TGCCCATCCC TCTGGACACA 
GCCCACTTGG ACCTGTCCTC CAACCGGCTG GAGATGGTGA ATGAGTCGGT GTTGGCGGGG 
CCGGGCTACA CGACGTTGGC TGGCCTGGAT CTCAGCCACA ACCTGCTCAC CAGCATCTCA 
CCCACTGCCT TCTCCCGCCT TCGCTACCTG GAGTCGCTTG ACCTCAGCCA CAATGGCCTG 
ACAGCCCTGC CAGCCGAGAG CTTCACCAGC TCACCCCTGA GCGACGTGAA CCTTAGCCAC 
AACCAGCTCC GGGAGGTCTC AGTGTCTGCC TTCACGACGC ACAGTCAGGG CCGGGCACTA 
CACGTGGACC TCTCCCACAA CCTCATTCAC CGCCTCGTGC CCCACCCCAC GAGGGCCGGC 
CTGCCTGCGC CCACCATTCA GAGCCTGAAC CTGGCCTGGA ACCGGCTCCA TGCCGTGCCC 
AACCTCCGAG ACTTGCCCCT GCGCTACCTG AGCCTGGATG GGAACCCTCT AGCTGTCATT 
GGTCCGGGTG CCTTCGCGGG GCTGGGAGGC CTTACACACC TGTCTCTGGC CAGCCTGCAG 
AGGCTCCCTG AGCTGGCGCC CAGTGGCTTC CGTGAGCTAC CGGGCCTGCA GGTCCTGGAC 
CTGTCGGGCA ACCCCAAGCT TAACTGGGCA GGAGCTGAGG TGTTTTCAGG CCTGAGCTCC 
CTGCAGGAGC TGGACCTTTC GGGCACCAAC CTGGTGCCCC TGCCTGAGGC GCTGCTCCTC 
CACCTCCCGG CACTGCAGAG CGTCAGCGTG GGCCAGGATG TGCGGTGCCG GCGCCTGGTG 
CGGGAGGGCA CCTACCCCCG GAGGCCTGGC TCCAGCCCCA AGGTGGCCCT GCACTGCGTA 
GACACCCGGG ATTCTGCTGC CAGGGGCCCC ACCATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSKU ( NM_001258210.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001245139.1 Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 2, mRNA.

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CloneID	OHu03186
Clone ID Related Accession (Same CDS sequence)	NM_015516.3 , NM_001318479.1 , NM_015516.4 , NM_001318478.1 , NM_001258210.1
Accession Version	NM_001258210.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Homo sapiens(human)
Product	tsukushin isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB199776.1 and BC020975.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 1, 2, 4, and 5 all encode isoform b. ##Evidence-Data-START## Transcript exon combination :: DA444888.1, DA866513.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGTGGC CCCTGCTGCT GCTGCTGGCC GTGAGTGGGG CCCAGACAAC CCGGCCATGC 
TTCCCCGGGT GCCAATGCGA GGTGGAGACC TTCGGCCTTT TCGACAGCTT CAGCCTGACT 
CGGGTGGATT GTAGCGGCCT GGGCCCCCAC ATCATGCCGG TGCCCATCCC TCTGGACACA 
GCCCACTTGG ACCTGTCCTC CAACCGGCTG GAGATGGTGA ATGAGTCGGT GTTGGCGGGG 
CCGGGCTACA CGACGTTGGC TGGCCTGGAT CTCAGCCACA ACCTGCTCAC CAGCATCTCA 
CCCACTGCCT TCTCCCGCCT TCGCTACCTG GAGTCGCTTG ACCTCAGCCA CAATGGCCTG 
ACAGCCCTGC CAGCCGAGAG CTTCACCAGC TCACCCCTGA GCGACGTGAA CCTTAGCCAC 
AACCAGCTCC GGGAGGTCTC AGTGTCTGCC TTCACGACGC ACAGTCAGGG CCGGGCACTA 
CACGTGGACC TCTCCCACAA CCTCATTCAC CGCCTCGTGC CCCACCCCAC GAGGGCCGGC 
CTGCCTGCGC CCACCATTCA GAGCCTGAAC CTGGCCTGGA ACCGGCTCCA TGCCGTGCCC 
AACCTCCGAG ACTTGCCCCT GCGCTACCTG AGCCTGGATG GGAACCCTCT AGCTGTCATT 
GGTCCGGGTG CCTTCGCGGG GCTGGGAGGC CTTACACACC TGTCTCTGGC CAGCCTGCAG 
AGGCTCCCTG AGCTGGCGCC CAGTGGCTTC CGTGAGCTAC CGGGCCTGCA GGTCCTGGAC 
CTGTCGGGCA ACCCCAAGCT TAACTGGGCA GGAGCTGAGG TGTTTTCAGG CCTGAGCTCC 
CTGCAGGAGC TGGACCTTTC GGGCACCAAC CTGGTGCCCC TGCCTGAGGC GCTGCTCCTC 
CACCTCCCGG CACTGCAGAG CGTCAGCGTG GGCCAGGATG TGCGGTGCCG GCGCCTGGTG 
CGGGAGGGCA CCTACCCCCG GAGGCCTGGC TCCAGCCCCA AGGTGGCCCT GCACTGCGTA 
GACACCCGGG ATTCTGCTGC CAGGGGCCCC ACCATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245139.1
CDS	130..1191
Translation	MPWPLLLLLAVSGAQTTRPCFPGCQCEVETFGLFDSFSLTRVDCSGLGPHIMPVPIPLDTAHLDLSSNRLEMVNESVLAGPGYTTLAGLDLSHNLLTSISPTAFSRLRYLESLDLSHNGLTALPAESFTSSPLSDVNLSHNQLREVSVSAFTTHSQGRALHVDLSHNLIHRLVPHPTRAGLPAPTIQSLNLAWNRLHAVPNLRDLPLRYLSLDGNPLAVIGPGAFAGLGGLTHLSLASLQRLPELAPSGFRELPGLQVLDLSGNPKLNWAGAEVFSGLSSLQELDLSGTNLVPLPEALLLHLPALQSVSVGQDVRCRRLVREGTYPRRPGSSPKVALHCVDTRDSAARGPTIL

Target ORF information:

RefSeq Version	NM_001258210.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258210.1

ORF Insert Sequence:

ATGCCGTGGC CCCTGCTGCT GCTGCTGGCC GTGAGTGGGG CCCAGACAAC CCGGCCATGC 
TTCCCCGGGT GCCAATGCGA GGTGGAGACC TTCGGCCTTT TCGACAGCTT CAGCCTGACT 
CGGGTGGATT GTAGCGGCCT GGGCCCCCAC ATCATGCCGG TGCCCATCCC TCTGGACACA 
GCCCACTTGG ACCTGTCCTC CAACCGGCTG GAGATGGTGA ATGAGTCGGT GTTGGCGGGG 
CCGGGCTACA CGACGTTGGC TGGCCTGGAT CTCAGCCACA ACCTGCTCAC CAGCATCTCA 
CCCACTGCCT TCTCCCGCCT TCGCTACCTG GAGTCGCTTG ACCTCAGCCA CAATGGCCTG 
ACAGCCCTGC CAGCCGAGAG CTTCACCAGC TCACCCCTGA GCGACGTGAA CCTTAGCCAC 
AACCAGCTCC GGGAGGTCTC AGTGTCTGCC TTCACGACGC ACAGTCAGGG CCGGGCACTA 
CACGTGGACC TCTCCCACAA CCTCATTCAC CGCCTCGTGC CCCACCCCAC GAGGGCCGGC 
CTGCCTGCGC CCACCATTCA GAGCCTGAAC CTGGCCTGGA ACCGGCTCCA TGCCGTGCCC 
AACCTCCGAG ACTTGCCCCT GCGCTACCTG AGCCTGGATG GGAACCCTCT AGCTGTCATT 
GGTCCGGGTG CCTTCGCGGG GCTGGGAGGC CTTACACACC TGTCTCTGGC CAGCCTGCAG 
AGGCTCCCTG AGCTGGCGCC CAGTGGCTTC CGTGAGCTAC CGGGCCTGCA GGTCCTGGAC 
CTGTCGGGCA ACCCCAAGCT TAACTGGGCA GGAGCTGAGG TGTTTTCAGG CCTGAGCTCC 
CTGCAGGAGC TGGACCTTTC GGGCACCAAC CTGGTGCCCC TGCCTGAGGC GCTGCTCCTC 
CACCTCCCGG CACTGCAGAG CGTCAGCGTG GGCCAGGATG TGCGGTGCCG GCGCCTGGTG 
CGGGAGGGCA CCTACCCCCG GAGGCCTGGC TCCAGCCCCA AGGTGGCCCT GCACTGCGTA 
GACACCCGGG ATTCTGCTGC CAGGGGCCCC ACCATCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSKU ( NM_001318477.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305406.1 Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu45266
Clone ID Related Accession (Same CDS sequence)	NM_001318477.1
Accession Version	NM_001318477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Homo sapiens(human)
Product	tsukushin isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK126536.1, AP003119.2 and BC020975.1. On Jan 8, 2016 this sequence version replaced XM_005273892.2. Transcript Variant: This variant (3) represents the longest transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: AK126536.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTGTTA GACCTTCCAC AGCCACGTGG AATTCCCCCA CCATGCCGTG GCCCCTGCTG 
CTGCTGCTGG CCGTGAGTGG GGCCCAGACA ACCCGGCCAT GCTTCCCCGG GTGCCAATGC 
GAGGTGGAGA CCTTCGGCCT TTTCGACAGC TTCAGCCTGA CTCGGGTGGA TTGTAGCGGC 
CTGGGCCCCC ACATCATGCC GGTGCCCATC CCTCTGGACA CAGCCCACTT GGACCTGTCC 
TCCAACCGGC TGGAGATGGT GAATGAGTCG GTGTTGGCGG GGCCGGGCTA CACGACGTTG 
GCTGGCCTGG ATCTCAGCCA CAACCTGCTC ACCAGCATCT CACCCACTGC CTTCTCCCGC 
CTTCGCTACC TGGAGTCGCT TGACCTCAGC CACAATGGCC TGACAGCCCT GCCAGCCGAG 
AGCTTCACCA GCTCACCCCT GAGCGACGTG AACCTTAGCC ACAACCAGCT CCGGGAGGTC 
TCAGTGTCTG CCTTCACGAC GCACAGTCAG GGCCGGGCAC TACACGTGGA CCTCTCCCAC 
AACCTCATTC ACCGCCTCGT GCCCCACCCC ACGAGGGCCG GCCTGCCTGC GCCCACCATT 
CAGAGCCTGA ACCTGGCCTG GAACCGGCTC CATGCCGTGC CCAACCTCCG AGACTTGCCC 
CTGCGCTACC TGAGCCTGGA TGGGAACCCT CTAGCTGTCA TTGGTCCGGG TGCCTTCGCG 
GGGCTGGGAG GCCTTACACA CCTGTCTCTG GCCAGCCTGC AGAGGCTCCC TGAGCTGGCG 
CCCAGTGGCT TCCGTGAGCT ACCGGGCCTG CAGGTCCTGG ACCTGTCGGG CAACCCCAAG 
CTTAACTGGG CAGGAGCTGA GGTGTTTTCA GGCCTGAGCT CCCTGCAGGA GCTGGACCTT 
TCGGGCACCA ACCTGGTGCC CCTGCCTGAG GCGCTGCTCC TCCACCTCCC GGCACTGCAG 
AGCGTCAGCG TGGGCCAGGA TGTGCGGTGC CGGCGCCTGG TGCGGGAGGG CACCTACCCC 
CGGAGGCCTG GCTCCAGCCC CAAGGTGGCC CTGCACTGCG TAGACACCCG GGATTCTGCT 
GCCAGGGGCC CCACCATCTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305406.1
CDS	985..2088
Translation	MIVRPSTATWNSPTMPWPLLLLLAVSGAQTTRPCFPGCQCEVETFGLFDSFSLTRVDCSGLGPHIMPVPIPLDTAHLDLSSNRLEMVNESVLAGPGYTTLAGLDLSHNLLTSISPTAFSRLRYLESLDLSHNGLTALPAESFTSSPLSDVNLSHNQLREVSVSAFTTHSQGRALHVDLSHNLIHRLVPHPTRAGLPAPTIQSLNLAWNRLHAVPNLRDLPLRYLSLDGNPLAVIGPGAFAGLGGLTHLSLASLQRLPELAPSGFRELPGLQVLDLSGNPKLNWAGAEVFSGLSSLQELDLSGTNLVPLPEALLLHLPALQSVSVGQDVRCRRLVREGTYPRRPGSSPKVALHCVDTRDSAARGPTIL

Target ORF information:

RefSeq Version	NM_001318477.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tsukushi, small leucine rich proteoglycan (TSKU), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318477.1

ORF Insert Sequence:

ATGATTGTTA GACCTTCCAC AGCCACGTGG AATTCCCCCA CCATGCCGTG GCCCCTGCTG 
CTGCTGCTGG CCGTGAGTGG GGCCCAGACA ACCCGGCCAT GCTTCCCCGG GTGCCAATGC 
GAGGTGGAGA CCTTCGGCCT TTTCGACAGC TTCAGCCTGA CTCGGGTGGA TTGTAGCGGC 
CTGGGCCCCC ACATCATGCC GGTGCCCATC CCTCTGGACA CAGCCCACTT GGACCTGTCC 
TCCAACCGGC TGGAGATGGT GAATGAGTCG GTGTTGGCGG GGCCGGGCTA CACGACGTTG 
GCTGGCCTGG ATCTCAGCCA CAACCTGCTC ACCAGCATCT CACCCACTGC CTTCTCCCGC 
CTTCGCTACC TGGAGTCGCT TGACCTCAGC CACAATGGCC TGACAGCCCT GCCAGCCGAG 
AGCTTCACCA GCTCACCCCT GAGCGACGTG AACCTTAGCC ACAACCAGCT CCGGGAGGTC 
TCAGTGTCTG CCTTCACGAC GCACAGTCAG GGCCGGGCAC TACACGTGGA CCTCTCCCAC 
AACCTCATTC ACCGCCTCGT GCCCCACCCC ACGAGGGCCG GCCTGCCTGC GCCCACCATT 
CAGAGCCTGA ACCTGGCCTG GAACCGGCTC CATGCCGTGC CCAACCTCCG AGACTTGCCC 
CTGCGCTACC TGAGCCTGGA TGGGAACCCT CTAGCTGTCA TTGGTCCGGG TGCCTTCGCG 
GGGCTGGGAG GCCTTACACA CCTGTCTCTG GCCAGCCTGC AGAGGCTCCC TGAGCTGGCG 
CCCAGTGGCT TCCGTGAGCT ACCGGGCCTG CAGGTCCTGG ACCTGTCGGG CAACCCCAAG 
CTTAACTGGG CAGGAGCTGA GGTGTTTTCA GGCCTGAGCT CCCTGCAGGA GCTGGACCTT 
TCGGGCACCA ACCTGGTGCC CCTGCCTGAG GCGCTGCTCC TCCACCTCCC GGCACTGCAG 
AGCGTCAGCG TGGGCCAGGA TGTGCGGTGC CGGCGCCTGG TGCGGGAGGG CACCTACCCC 
CGGAGGCCTG GCTCCAGCCC CAAGGTGGCC CTGCACTGCG TAGACACCCG GGATTCTGCT 
GCCAGGGGCC CCACCATCTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tsukushi controls the hair cycle by regulating TGF-?1 signaling.
Developmental biology372(1)81-7(2012 Dec)
Niimori D,Kawano R,Felemban A,Niimori-Kita K,Tanaka H,Ihn H,Ohta K

Stromal expression of decorin, Semaphorin6D, SPARC, Sprouty1 and Tsukushi in developing prostate and decreased levels of decorin in prostate cancer.
PloS one7(8)e42516(2012)
Henke A,Grace OC,Ashley GR,Stewart GD,Riddick AC,Yeun H,O'Donnell M,Anderson RA,Thomson AA

Comparative analysis of gene regulation by the transcription factor PPARalpha between mouse and human.
PloS one4(8)e6796(2009 Aug)
Rakhshandehroo M,Hooiveld G,M?ller M,Kersten S

Functional proteomics mapping of a human signaling pathway.
Genome research14(7)1324-32(2004 Jul)
Colland F,Jacq X,Trouplin V,Mougin C,Groizeleau C,Hamburger A,Meil A,Wojcik J,Legrain P,Gauthier JM

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TSKU cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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FAX

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