ARIH1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARIH1
Entrez Gene ID	25820
Full Name	ariadne RBR E3 ubiquitin protein ligase 1
Synonyms	ARI,HARI,HHARI,UBCH7BP
General protein information	Preferred Names ariadne RBR E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase ARIH1 ARI-1 H7-AP2 MOP-6 ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 ariadne, Drosophila, homolog of monocyte protein 6 protein ariadne-1 homolog ubcH7-binding protein ubcM4-interacting protein ubiquitin-conjugating enzyme E2-binding protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q24.1
Disorder MIM:	605624

mRNA and Protein(s)

mRNA	Protein	Name
NM_005744.3	NP_005735.2	E3 ubiquitin-protein ligase ARIH1
XM_011521439.3	XP_011519741.1	E3 ubiquitin-protein ligase ARIH1 isoform X2
XM_011521438.2	XP_011519740.1	E3 ubiquitin-protein ligase ARIH1 isoform X1
NM_005744.5	NP_005735.2	E3 ubiquitin-protein ligase ARIH1
XM_011521439.2	XP_011519741.1	E3 ubiquitin-protein ligase ARIH1 isoform X2
NM_005744.4	NP_005735.2	E3 ubiquitin-protein ligase ARIH1
XM_011521438.3	XP_011519740.1	E3 ubiquitin-protein ligase ARIH1 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ARIH1	NP_001025558.1
Pan troglodytes (chimpanzee)	ARIH1	NP_001231574.1
	LOC702912	XP_001091222.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010891	XP_309801.3
Schizosaccharomyces pombe (fission yeast)	SPAC328.02	NP_594204.1
Neurospora crassa	NCU06882	XP_959229.2
Oryza sativa (rice)	Os03g0233500	NP_001049474.1
Bos taurus (cattle)	ARIH1	NP_001075183.1
Rattus norvegicus (Norway rat)	Arih1	NP_001013126.2
Arabidopsis thaliana (thale cress)	ARI1	NP_567966.1
Arabidopsis thaliana (thale cress)	ARI2	NP_179206.2
Homo sapiens (human)	ARIH1	NP_005735.2
Canis lupus familiaris (dog)	ARIH1	XP_535533.2
Mus musculus (house mouse)	Arih1	NP_064311.2
Magnaporthe oryzae (rice blast fungus)	MGG_00859	XP_003718113.1
Danio rerio (zebrafish)	arih1l	NP_998088.1
Danio rerio (zebrafish)	arih1	NP_956052.1
Drosophila melanogaster (fruit fly)	ari-1	NP_728145.1
Xenopus tropicalis (tropical clawed frog)	arih1	NP_001116952.1

Gene Ontology
Bibliography

Related articles in PubMed

Parkin-Independent Mitophagy Controls Chemotherapeutic Response in Cancer Cells.
Villa E, Pro?cs E, Rubio-Pati?o C, Obba S, Zunino B, Bossowski JP, Rozier RM, Chiche J, Mondrag?n L, Riley JS, Marchetti S, Verhoeyen E, Tait SWG, Ricci JE
Cell reports20(12)2846-2859(2017 Sep)

Structural insights into the mechanism and E2 specificity of the RBR E3 ubiquitin ligase HHARI.
Yuan L, Lv Z, Atkison JH, Olsen SK
Nature communications8(1)211(2017 08)

Structural Studies of HHARI/UbcH7?Ub Reveal Unique E2?Ub Conformational Restriction by RBR RING1.
Dove KK, Olszewski JL, Martino L, Duda DM, Wu XS, Miller DJ, Reiter KH, Rittinger K, Schulman BA, Klevit RE
Structure (London, England : 1993)25(6)890-900.e5(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HHARI prevents spurious discharge of Ub from E2 to lysine residues by harboring structural elements that block E2 ~ Ub from adopting a 'closed' conformation.
Title: Structural insights into the mechanism and E2 specificity of the RBR E3 ubiquitin ligase HHARI.

DNA damage induces an increase in ARIH1 protein levels and association of ARIH1 with 4EHP. In turn, this causes 4EHP recruitment to the mRNA cap, where it is known to compete with eIF4E.
Title: The E3 ubiquitin ligase ARIH1 protects against genotoxic stress by initiating a 4EHP-mediated mRNA translation arrest.

TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes.
Title: TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes.

HHARI is a marker of cellular proliferation associated with nuclear bodies.
Title: Human Homolog of Drosophila Ariadne (HHARI) is a marker of cellular proliferation associated with nuclear bodies.

Determined is the three-dimensional solution structure of the catalytic RING2 domain from HHARI. It shows glimpses of a HECT E3 ligase.
Title: Structure of the HHARI catalytic domain shows glimpses of a HECT E3 ligase.

The following ARIH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARIH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30640	NM_005744.5 Latest version!	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu66508	XM_011521439.3 Latest version!	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu66507	XM_011521438.3 Latest version!	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu30640	NM_005744.3	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu66508	XM_011521439.2	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu30640	NM_005744.4	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu66507	XM_011521438.2	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARIH1 ( NM_005744.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005735.2 Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), mRNA.

Price & Availability↑

CloneID	OHu30640
Clone ID Related Accession (Same CDS sequence)	NM_005744.3 , NM_005744.5 , NM_005744.4
Accession Version	NM_005744.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase ARIH1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ243190.1, BC051877.1, AJ009771.1, AC100827.2 and AL117650.1. On May 8, 2008 this sequence version replaced NM_005744.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ243190.1, AJ130976.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCGG ACGAGGGCTA CAACTACGAG TTCGACGAGG ACGAGGAGTG CAGTGAGGAG 
GACAGCGGCG CCGAGGAGGA GGAGGACGAA GACGACGACG AGCCGGACGA TGATACCCTG 
GATCTGGGCG AGGTGGAGCT GGTGGAGCCC GGGCTGGGCG TCGGCGGGGA GCGGGACGGA 
CTGCTGTGCG GGGAGACGGG CGGTGGCGGC GGCAGCGCTC TGGGGCCCGG CGGTGGCGGC 
GGCGGCGGCG GCGGCGGTGG TGGTGGCGGG CCGGGGCATG AGCAGGAGGA GGATTACCGC 
TACGAGGTGC TCACGGCCGA GCAGATTCTA CAACACATGG TGGAATGTAT CCGGGAGGTC 
AACGAGGTCA TCCAGAATCC AGCAACTATC ACAAGAATAC TCCTTAGCCA CTTCAATTGG 
GATAAAGAGA AGCTAATGGA AAGGTACTTT GATGGAAACC TGGAGAAGCT CTTTGCTGAG 
TGTCATGTAA TTAATCCAAG TAAAAAGTCT CGAACACGCC AGATGAATAC AAGGTCATCA 
GCACAGGATA TGCCTTGTCA GATCTGCTAC TTGAACTACC CTAACTCGTA TTTCACTGGC 
CTTGAATGTG GACATAAGTT TTGTATGCAG TGCTGGAGTG AATATTTAAC TACCAAAATA 
ATGGAAGAAG GCATGGGTCA GACTATTTCG TGTCCTGCTC ATGGTTGTGA TATCTTAGTG 
GATGACAACA CAGTTATGCG CCTGATCACA GATTCAAAAG TTAAATTAAA GTATCAGCAT 
TTAATAACAA ATAGCTTTGT AGAGTGCAAT CGACTGTTAA AGTGGTGTCC TGCCCCAGAT 
TGCCACCATG TTGTTAAAGT CCAATATCCT GATGCTAAAC CTGTTCGCTG CAAATGTGGG 
CGCCAATTTT GCTTTAACTG TGGAGAAAAT TGGCATGATC CTGTTAAATG TAAGTGGTTA 
AAGAAATGGA TTAAAAAGTG TGATGATGAC AGTGAAACCT CCAATTGGAT TGCAGCCAAC 
ACAAAGGAAT GTCCCAAATG CCATGTCACA ATTGAGAAGG ATGGTGGTTG TAATCACATG 
GTCTGTCGTA ACCAGAATTG TAAAGCAGAG TTTTGCTGGG TGTGTCTTGG CCCATGGGAA 
CCACATGGAT CTGCCTGGTA CAACTGTAAC CGCTATAATG AGGATGATGC AAAGGCAGCA 
AGAGATGCAC AGGAGCGATC TAGGGCAGCC CTGCAGAGGT ACCTGTTCTA CTGTAATCGC 
TATATGAACC ACATGCAGAG CCTGCGCTTT GAGCACAAAC TATATGCTCA GGTGAAACAG 
AAAATGGAGG AGATGCAGCA GCACAACATG TCCTGGATTG AGGTGCAGTT CCTGAAGAAG 
GCAGTTGATG TCCTCTGCCA GTGTCGTGCC ACACTCATGT ACACTTATGT CTTCGCTTTC 
TACCTCAAAA AGAATAACCA GTCCATTATC TTTGAGAATA ACCAAGCAGA TCTAGAGAAT 
GCCACAGAGG TGCTCTCGGG CTACCTTGAA CGAGATATTT CCCAAGATTC TCTGCAGGAT 
ATAAAGCAGA AAGTACAAGA CAAGTACAGA TACTGTGAGA GTCGACGAAG GGTTTTGTTA 
CAGCATGTGC ATGAAGGCTA TGAAAAAGAT CTGTGGGAGT ACATTGAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005735.2
CDS	315..1988
Translation	MDSDEGYNYEFDEDEECSEEDSGAEEEEDEDDDEPDDDTLDLGEVELVEPGLGVGGERDGLLCGETGGGGGSALGPGGGGGGGGGGGGGGPGHEQEEDYRYEVLTAEQILQHMVECIREVNEVIQNPATITRILLSHFNWDKEKLMERYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIED

Target ORF information:

RefSeq Version	NM_005744.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005744.3

ORF Insert Sequence:

ATGGACTCGG ACGAGGGCTA CAACTACGAG TTCGACGAGG ACGAGGAGTG CAGTGAGGAG 
GACAGCGGCG CCGAGGAGGA GGAGGACGAA GACGACGACG AGCCGGACGA TGATACCCTG 
GATCTGGGCG AGGTGGAGCT GGTGGAGCCC GGGCTGGGCG TCGGCGGGGA GCGGGACGGA 
CTGCTGTGCG GGGAGACGGG CGGTGGCGGC GGCAGCGCTC TGGGGCCCGG CGGTGGCGGC 
GGCGGCGGCG GCGGCGGTGG TGGTGGCGGG CCGGGGCATG AGCAGGAGGA GGATTACCGC 
TACGAGGTGC TCACGGCCGA GCAGATTCTA CAACACATGG TGGAATGTAT CCGGGAGGTC 
AACGAGGTCA TCCAGAATCC AGCAACTATC ACAAGAATAC TCCTTAGCCA CTTCAATTGG 
GATAAAGAGA AGCTAATGGA AAGGTACTTT GATGGAAACC TGGAGAAGCT CTTTGCTGAG 
TGTCATGTAA TTAATCCAAG TAAAAAGTCT CGAACACGCC AGATGAATAC AAGGTCATCA 
GCACAGGATA TGCCTTGTCA GATCTGCTAC TTGAACTACC CTAACTCGTA TTTCACTGGC 
CTTGAATGTG GACATAAGTT TTGTATGCAG TGCTGGAGTG AATATTTAAC TACCAAAATA 
ATGGAAGAAG GCATGGGTCA GACTATTTCG TGTCCTGCTC ATGGTTGTGA TATCTTAGTG 
GATGACAACA CAGTTATGCG CCTGATCACA GATTCAAAAG TTAAATTAAA GTATCAGCAT 
TTAATAACAA ATAGCTTTGT AGAGTGCAAT CGACTGTTAA AGTGGTGTCC TGCCCCAGAT 
TGCCACCATG TTGTTAAAGT CCAATATCCT GATGCTAAAC CTGTTCGCTG CAAATGTGGG 
CGCCAATTTT GCTTTAACTG TGGAGAAAAT TGGCATGATC CTGTTAAATG TAAGTGGTTA 
AAGAAATGGA TTAAAAAGTG TGATGATGAC AGTGAAACCT CCAATTGGAT TGCAGCCAAC 
ACAAAGGAAT GTCCCAAATG CCATGTCACA ATTGAGAAGG ATGGTGGTTG TAATCACATG 
GTCTGTCGTA ACCAGAATTG TAAAGCAGAG TTTTGCTGGG TGTGTCTTGG CCCATGGGAA 
CCACATGGAT CTGCCTGGTA CAACTGTAAC CGCTATAATG AGGATGATGC AAAGGCAGCA 
AGAGATGCAC AGGAGCGATC TAGGGCAGCC CTGCAGAGGT ACCTGTTCTA CTGTAATCGC 
TATATGAACC ACATGCAGAG CCTGCGCTTT GAGCACAAAC TATATGCTCA GGTGAAACAG 
AAAATGGAGG AGATGCAGCA GCACAACATG TCCTGGATTG AGGTGCAGTT CCTGAAGAAG 
GCAGTTGATG TCCTCTGCCA GTGTCGTGCC ACACTCATGT ACACTTATGT CTTCGCTTTC 
TACCTCAAAA AGAATAACCA GTCCATTATC TTTGAGAATA ACCAAGCAGA TCTAGAGAAT 
GCCACAGAGG TGCTCTCGGG CTACCTTGAA CGAGATATTT CCCAAGATTC TCTGCAGGAT 
ATAAAGCAGA AAGTACAAGA CAAGTACAGA TACTGTGAGA GTCGACGAAG GGTTTTGTTA 
CAGCATGTGC ATGAAGGCTA TGAAAAAGAT CTGTGGGAGT ACATTGAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARIH1 ( NM_005744.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005735.2 Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), mRNA.

Price & Availability↑

CloneID	OHu30640
Clone ID Related Accession (Same CDS sequence)	NM_005744.3 , NM_005744.5 , NM_005744.4
Accession Version	NM_005744.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-29
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase ARIH1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC079322.5 and AC100827.2. On May 17, 2019 this sequence version replaced NM_005744.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ243190.1, AJ130976.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000379887.9/ ENSP00000369217.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACTCGG ACGAGGGCTA CAACTACGAG TTCGACGAGG ACGAGGAGTG CAGTGAGGAG 
GACAGCGGCG CCGAGGAGGA GGAGGACGAA GACGACGACG AGCCGGACGA TGATACCCTG 
GATCTGGGCG AGGTGGAGCT GGTGGAGCCC GGGCTGGGCG TCGGCGGGGA GCGGGACGGA 
CTGCTGTGCG GGGAGACGGG CGGTGGCGGC GGCAGCGCTC TGGGGCCCGG CGGTGGCGGC 
GGCGGCGGCG GCGGCGGTGG TGGTGGCGGG CCGGGGCATG AGCAGGAGGA GGATTACCGC 
TACGAGGTGC TCACGGCCGA GCAGATTCTA CAACACATGG TGGAATGTAT CCGGGAGGTC 
AACGAGGTCA TCCAGAATCC AGCAACTATC ACAAGAATAC TCCTTAGCCA CTTCAATTGG 
GATAAAGAGA AGCTAATGGA AAGGTACTTT GATGGAAACC TGGAGAAGCT CTTTGCTGAG 
TGTCATGTAA TTAATCCAAG TAAAAAGTCT CGAACACGCC AGATGAATAC AAGGTCATCA 
GCACAGGATA TGCCTTGTCA GATCTGCTAC TTGAACTACC CTAACTCGTA TTTCACTGGC 
CTTGAATGTG GACATAAGTT TTGTATGCAG TGCTGGAGTG AATATTTAAC TACCAAAATA 
ATGGAAGAAG GCATGGGTCA GACTATTTCG TGTCCTGCTC ATGGTTGTGA TATCTTAGTG 
GATGACAACA CAGTTATGCG CCTGATCACA GATTCAAAAG TTAAATTAAA GTATCAGCAT 
TTAATAACAA ATAGCTTTGT AGAGTGCAAT CGACTGTTAA AGTGGTGTCC TGCCCCAGAT 
TGCCACCATG TTGTTAAAGT CCAATATCCT GATGCTAAAC CTGTTCGCTG CAAATGTGGG 
CGCCAATTTT GCTTTAACTG TGGAGAAAAT TGGCATGATC CTGTTAAATG TAAGTGGTTA 
AAGAAATGGA TTAAAAAGTG TGATGATGAC AGTGAAACCT CCAATTGGAT TGCAGCCAAC 
ACAAAGGAAT GTCCCAAATG CCATGTCACA ATTGAGAAGG ATGGTGGTTG TAATCACATG 
GTCTGTCGTA ACCAGAATTG TAAAGCAGAG TTTTGCTGGG TGTGTCTTGG CCCATGGGAA 
CCACATGGAT CTGCCTGGTA CAACTGTAAC CGCTATAATG AGGATGATGC AAAGGCAGCA 
AGAGATGCAC AGGAGCGATC TAGGGCAGCC CTGCAGAGGT ACCTGTTCTA CTGTAATCGC 
TATATGAACC ACATGCAGAG CCTGCGCTTT GAGCACAAAC TATATGCTCA GGTGAAACAG 
AAAATGGAGG AGATGCAGCA GCACAACATG TCCTGGATTG AGGTGCAGTT CCTGAAGAAG 
GCAGTTGATG TCCTCTGCCA GTGTCGTGCC ACACTCATGT ACACTTATGT CTTCGCTTTC 
TACCTCAAAA AGAATAACCA GTCCATTATC TTTGAGAATA ACCAAGCAGA TCTAGAGAAT 
GCCACAGAGG TGCTCTCGGG CTACCTTGAA CGAGATATTT CCCAAGATTC TCTGCAGGAT 
ATAAAGCAGA AAGTACAAGA CAAGTACAGA TACTGTGAGA GTCGACGAAG GGTTTTGTTA 
CAGCATGTGC ATGAAGGCTA TGAAAAAGAT CTGTGGGAGT ACATTGAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005735.2
CDS	311..1984
Translation	MDSDEGYNYEFDEDEECSEEDSGAEEEEDEDDDEPDDDTLDLGEVELVEPGLGVGGERDGLLCGETGGGGGSALGPGGGGGGGGGGGGGGPGHEQEEDYRYEVLTAEQILQHMVECIREVNEVIQNPATITRILLSHFNWDKEKLMERYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIED

Target ORF information:

RefSeq Version	NM_005744.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005744.5

ORF Insert Sequence:

ATGGACTCGG ACGAGGGCTA CAACTACGAG TTCGACGAGG ACGAGGAGTG CAGTGAGGAG 
GACAGCGGCG CCGAGGAGGA GGAGGACGAA GACGACGACG AGCCGGACGA TGATACCCTG 
GATCTGGGCG AGGTGGAGCT GGTGGAGCCC GGGCTGGGCG TCGGCGGGGA GCGGGACGGA 
CTGCTGTGCG GGGAGACGGG CGGTGGCGGC GGCAGCGCTC TGGGGCCCGG CGGTGGCGGC 
GGCGGCGGCG GCGGCGGTGG TGGTGGCGGG CCGGGGCATG AGCAGGAGGA GGATTACCGC 
TACGAGGTGC TCACGGCCGA GCAGATTCTA CAACACATGG TGGAATGTAT CCGGGAGGTC 
AACGAGGTCA TCCAGAATCC AGCAACTATC ACAAGAATAC TCCTTAGCCA CTTCAATTGG 
GATAAAGAGA AGCTAATGGA AAGGTACTTT GATGGAAACC TGGAGAAGCT CTTTGCTGAG 
TGTCATGTAA TTAATCCAAG TAAAAAGTCT CGAACACGCC AGATGAATAC AAGGTCATCA 
GCACAGGATA TGCCTTGTCA GATCTGCTAC TTGAACTACC CTAACTCGTA TTTCACTGGC 
CTTGAATGTG GACATAAGTT TTGTATGCAG TGCTGGAGTG AATATTTAAC TACCAAAATA 
ATGGAAGAAG GCATGGGTCA GACTATTTCG TGTCCTGCTC ATGGTTGTGA TATCTTAGTG 
GATGACAACA CAGTTATGCG CCTGATCACA GATTCAAAAG TTAAATTAAA GTATCAGCAT 
TTAATAACAA ATAGCTTTGT AGAGTGCAAT CGACTGTTAA AGTGGTGTCC TGCCCCAGAT 
TGCCACCATG TTGTTAAAGT CCAATATCCT GATGCTAAAC CTGTTCGCTG CAAATGTGGG 
CGCCAATTTT GCTTTAACTG TGGAGAAAAT TGGCATGATC CTGTTAAATG TAAGTGGTTA 
AAGAAATGGA TTAAAAAGTG TGATGATGAC AGTGAAACCT CCAATTGGAT TGCAGCCAAC 
ACAAAGGAAT GTCCCAAATG CCATGTCACA ATTGAGAAGG ATGGTGGTTG TAATCACATG 
GTCTGTCGTA ACCAGAATTG TAAAGCAGAG TTTTGCTGGG TGTGTCTTGG CCCATGGGAA 
CCACATGGAT CTGCCTGGTA CAACTGTAAC CGCTATAATG AGGATGATGC AAAGGCAGCA 
AGAGATGCAC AGGAGCGATC TAGGGCAGCC CTGCAGAGGT ACCTGTTCTA CTGTAATCGC 
TATATGAACC ACATGCAGAG CCTGCGCTTT GAGCACAAAC TATATGCTCA GGTGAAACAG 
AAAATGGAGG AGATGCAGCA GCACAACATG TCCTGGATTG AGGTGCAGTT CCTGAAGAAG 
GCAGTTGATG TCCTCTGCCA GTGTCGTGCC ACACTCATGT ACACTTATGT CTTCGCTTTC 
TACCTCAAAA AGAATAACCA GTCCATTATC TTTGAGAATA ACCAAGCAGA TCTAGAGAAT 
GCCACAGAGG TGCTCTCGGG CTACCTTGAA CGAGATATTT CCCAAGATTC TCTGCAGGAT 
ATAAAGCAGA AAGTACAAGA CAAGTACAGA TACTGTGAGA GTCGACGAAG GGTTTTGTTA 
CAGCATGTGC ATGAAGGCTA TGAAAAAGAT CTGTGGGAGT ACATTGAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARIH1 ( NM_005744.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005735.2 Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), mRNA.

Price & Availability↑

CloneID	OHu30640
Clone ID Related Accession (Same CDS sequence)	NM_005744.3 , NM_005744.5 , NM_005744.4
Accession Version	NM_005744.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase ARIH1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC079322.5 and AC100827.2. On Nov 30, 2018 this sequence version replaced NM_005744.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ130976.1, AJ243190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCGG ACGAGGGCTA CAACTACGAG TTCGACGAGG ACGAGGAGTG CAGTGAGGAG 
GACAGCGGCG CCGAGGAGGA GGAGGACGAA GACGACGACG AGCCGGACGA TGATACCCTG 
GATCTGGGCG AGGTGGAGCT GGTGGAGCCC GGGCTGGGCG TCGGCGGGGA GCGGGACGGA 
CTGCTGTGCG GGGAGACGGG CGGTGGCGGC GGCAGCGCTC TGGGGCCCGG CGGTGGCGGC 
GGCGGCGGCG GCGGCGGTGG TGGTGGCGGG CCGGGGCATG AGCAGGAGGA GGATTACCGC 
TACGAGGTGC TCACGGCCGA GCAGATTCTA CAACACATGG TGGAATGTAT CCGGGAGGTC 
AACGAGGTCA TCCAGAATCC AGCAACTATC ACAAGAATAC TCCTTAGCCA CTTCAATTGG 
GATAAAGAGA AGCTAATGGA AAGGTACTTT GATGGAAACC TGGAGAAGCT CTTTGCTGAG 
TGTCATGTAA TTAATCCAAG TAAAAAGTCT CGAACACGCC AGATGAATAC AAGGTCATCA 
GCACAGGATA TGCCTTGTCA GATCTGCTAC TTGAACTACC CTAACTCGTA TTTCACTGGC 
CTTGAATGTG GACATAAGTT TTGTATGCAG TGCTGGAGTG AATATTTAAC TACCAAAATA 
ATGGAAGAAG GCATGGGTCA GACTATTTCG TGTCCTGCTC ATGGTTGTGA TATCTTAGTG 
GATGACAACA CAGTTATGCG CCTGATCACA GATTCAAAAG TTAAATTAAA GTATCAGCAT 
TTAATAACAA ATAGCTTTGT AGAGTGCAAT CGACTGTTAA AGTGGTGTCC TGCCCCAGAT 
TGCCACCATG TTGTTAAAGT CCAATATCCT GATGCTAAAC CTGTTCGCTG CAAATGTGGG 
CGCCAATTTT GCTTTAACTG TGGAGAAAAT TGGCATGATC CTGTTAAATG TAAGTGGTTA 
AAGAAATGGA TTAAAAAGTG TGATGATGAC AGTGAAACCT CCAATTGGAT TGCAGCCAAC 
ACAAAGGAAT GTCCCAAATG CCATGTCACA ATTGAGAAGG ATGGTGGTTG TAATCACATG 
GTCTGTCGTA ACCAGAATTG TAAAGCAGAG TTTTGCTGGG TGTGTCTTGG CCCATGGGAA 
CCACATGGAT CTGCCTGGTA CAACTGTAAC CGCTATAATG AGGATGATGC AAAGGCAGCA 
AGAGATGCAC AGGAGCGATC TAGGGCAGCC CTGCAGAGGT ACCTGTTCTA CTGTAATCGC 
TATATGAACC ACATGCAGAG CCTGCGCTTT GAGCACAAAC TATATGCTCA GGTGAAACAG 
AAAATGGAGG AGATGCAGCA GCACAACATG TCCTGGATTG AGGTGCAGTT CCTGAAGAAG 
GCAGTTGATG TCCTCTGCCA GTGTCGTGCC ACACTCATGT ACACTTATGT CTTCGCTTTC 
TACCTCAAAA AGAATAACCA GTCCATTATC TTTGAGAATA ACCAAGCAGA TCTAGAGAAT 
GCCACAGAGG TGCTCTCGGG CTACCTTGAA CGAGATATTT CCCAAGATTC TCTGCAGGAT 
ATAAAGCAGA AAGTACAAGA CAAGTACAGA TACTGTGAGA GTCGACGAAG GGTTTTGTTA 
CAGCATGTGC ATGAAGGCTA TGAAAAAGAT CTGTGGGAGT ACATTGAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005735.2
CDS	311..1984
Translation	MDSDEGYNYEFDEDEECSEEDSGAEEEEDEDDDEPDDDTLDLGEVELVEPGLGVGGERDGLLCGETGGGGGSALGPGGGGGGGGGGGGGGPGHEQEEDYRYEVLTAEQILQHMVECIREVNEVIQNPATITRILLSHFNWDKEKLMERYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIED

Target ORF information:

RefSeq Version	NM_005744.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005744.4

ORF Insert Sequence:

ATGGACTCGG ACGAGGGCTA CAACTACGAG TTCGACGAGG ACGAGGAGTG CAGTGAGGAG 
GACAGCGGCG CCGAGGAGGA GGAGGACGAA GACGACGACG AGCCGGACGA TGATACCCTG 
GATCTGGGCG AGGTGGAGCT GGTGGAGCCC GGGCTGGGCG TCGGCGGGGA GCGGGACGGA 
CTGCTGTGCG GGGAGACGGG CGGTGGCGGC GGCAGCGCTC TGGGGCCCGG CGGTGGCGGC 
GGCGGCGGCG GCGGCGGTGG TGGTGGCGGG CCGGGGCATG AGCAGGAGGA GGATTACCGC 
TACGAGGTGC TCACGGCCGA GCAGATTCTA CAACACATGG TGGAATGTAT CCGGGAGGTC 
AACGAGGTCA TCCAGAATCC AGCAACTATC ACAAGAATAC TCCTTAGCCA CTTCAATTGG 
GATAAAGAGA AGCTAATGGA AAGGTACTTT GATGGAAACC TGGAGAAGCT CTTTGCTGAG 
TGTCATGTAA TTAATCCAAG TAAAAAGTCT CGAACACGCC AGATGAATAC AAGGTCATCA 
GCACAGGATA TGCCTTGTCA GATCTGCTAC TTGAACTACC CTAACTCGTA TTTCACTGGC 
CTTGAATGTG GACATAAGTT TTGTATGCAG TGCTGGAGTG AATATTTAAC TACCAAAATA 
ATGGAAGAAG GCATGGGTCA GACTATTTCG TGTCCTGCTC ATGGTTGTGA TATCTTAGTG 
GATGACAACA CAGTTATGCG CCTGATCACA GATTCAAAAG TTAAATTAAA GTATCAGCAT 
TTAATAACAA ATAGCTTTGT AGAGTGCAAT CGACTGTTAA AGTGGTGTCC TGCCCCAGAT 
TGCCACCATG TTGTTAAAGT CCAATATCCT GATGCTAAAC CTGTTCGCTG CAAATGTGGG 
CGCCAATTTT GCTTTAACTG TGGAGAAAAT TGGCATGATC CTGTTAAATG TAAGTGGTTA 
AAGAAATGGA TTAAAAAGTG TGATGATGAC AGTGAAACCT CCAATTGGAT TGCAGCCAAC 
ACAAAGGAAT GTCCCAAATG CCATGTCACA ATTGAGAAGG ATGGTGGTTG TAATCACATG 
GTCTGTCGTA ACCAGAATTG TAAAGCAGAG TTTTGCTGGG TGTGTCTTGG CCCATGGGAA 
CCACATGGAT CTGCCTGGTA CAACTGTAAC CGCTATAATG AGGATGATGC AAAGGCAGCA 
AGAGATGCAC AGGAGCGATC TAGGGCAGCC CTGCAGAGGT ACCTGTTCTA CTGTAATCGC 
TATATGAACC ACATGCAGAG CCTGCGCTTT GAGCACAAAC TATATGCTCA GGTGAAACAG 
AAAATGGAGG AGATGCAGCA GCACAACATG TCCTGGATTG AGGTGCAGTT CCTGAAGAAG 
GCAGTTGATG TCCTCTGCCA GTGTCGTGCC ACACTCATGT ACACTTATGT CTTCGCTTTC 
TACCTCAAAA AGAATAACCA GTCCATTATC TTTGAGAATA ACCAAGCAGA TCTAGAGAAT 
GCCACAGAGG TGCTCTCGGG CTACCTTGAA CGAGATATTT CCCAAGATTC TCTGCAGGAT 
ATAAAGCAGA AAGTACAAGA CAAGTACAGA TACTGTGAGA GTCGACGAAG GGTTTTGTTA 
CAGCATGTGC ATGAAGGCTA TGAAAAAGAT CTGTGGGAGT ACATTGAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARIH1 ( XM_011521439.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519741.1 Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), transcript variant X2, mRNA.

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CloneID	OHu66508
Clone ID Related Accession (Same CDS sequence)	XM_011521439.3 , XM_011521439.2
Accession Version	XM_011521439.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase ARIH1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521439.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCCGG GAGGTCAACG AGGTCATCCA GTGCATATTG GGTACTTCCA GAAGTATGAA 
TTCAGTGATG CAAAGGACAG GCGGGCTGCA GCAGCAAAGA ATCCAGCAAC TATCACAAGA 
ATACTCCTTA GCCACTTCAA TTGGGATAAA GAGAAGCTAA TGGAAAGGTA CTTTGATGGA 
AACCTGGAGA AGCTCTTTGC TGAGTGTCAT GTAATTAATC CAAGTAAAAA GTCTCGAACA 
CGCCAGATGA ATACAAGGTC ATCAGCACAG GATATGCCTT GTCAGATCTG CTACTTGAAC 
TACCCTAACT CGTATTTCAC TGGCCTTGAA TGTGGACATA AGTTTTGTAT GCAGTGCTGG 
AGTGAATATT TAACTACCAA AATAATGGAA GAAGGCATGG GTCAGACTAT TTCGTGTCCT 
GCTCATGGTT GTGATATCTT AGTGGATGAC AACACAGTTA TGCGCCTGAT CACAGATTCA 
AAAGTTAAAT TAAAGTATCA GCATTTAATA ACAAATAGCT TTGTAGAGTG CAATCGACTG 
TTAAAGTGGT GTCCTGCCCC AGATTGCCAC CATGTTGTTA AAGTCCAATA TCCTGATGCT 
AAACCTGTTC GCTGCAAATG TGGGCGCCAA TTTTGCTTTA ACTGTGGAGA AAATTGGCAT 
GATCCTGTTA AATGTAAGTG GTTAAAGAAA TGGATTAAAA AGTGTGATGA TGACAGTGAA 
ACCTCCAATT GGATTGCAGC CAACACAAAG GAATGTCCCA AATGCCATGT CACAATTGAG 
AAGGATGGTG GTTGTAATCA CATGGTCTGT CGTAACCAGA ATTGTAAAGC AGAGTTTTGC 
TGGGTGTGTC TTGGCCCATG GGAACCACAT GGATCTGCCT GGTACAACTG TAACCGCTAT 
AATGAGGATG ATGCAAAGGC AGCAAGAGAT GCACAGGAGC GATCTAGGGC AGCCCTGCAG 
AGGTACCTGT TCTACTGTAA TCGCTATATG AACCACATGC AGAGCCTGCG CTTTGAGCAC 
AAACTATATG CTCAGGTGAA ACAGAAAATG GAGGAGATGC AGCAGCACAA CATGTCCTGG 
ATTGAGGTGC AGTTCCTGAA GAAGGCAGTT GATGTCCTCT GCCAGTGTCG TGCCACACTC 
ATGTACACTT ATGTCTTCGC TTTCTACCTC AAAAAGAATA ACCAGTCCAT TATCTTTGAG 
AATAACCAAG CAGATCTAGA GAATGCCACA GAGGTGCTCT CGGGCTACCT TGAACGAGAT 
ATTTCCCAAG ATTCTCTGCA GGATATAAAG CAGAAAGTAC AAGACAAGTA CAGATACTGT 
GAGAGTCGAC GAAGGGTTTT GTTACAGCAT GTGCATGAAG GCTATGAAAA AGATCTGTGG 
GAGTACATTG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519741.1
CDS	105..1502
Translation	MYPGGQRGHPVHIGYFQKYEFSDAKDRRAAAAKNPATITRILLSHFNWDKEKLMERYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIED

Target ORF information:

RefSeq Version	XM_011521439.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521439.3

ORF Insert Sequence:

ATGTATCCGG GAGGTCAACG AGGTCATCCA GTGCATATTG GGTACTTCCA GAAGTATGAA 
TTCAGTGATG CAAAGGACAG GCGGGCTGCA GCAGCAAAGA ATCCAGCAAC TATCACAAGA 
ATACTCCTTA GCCACTTCAA TTGGGATAAA GAGAAGCTAA TGGAAAGGTA CTTTGATGGA 
AACCTGGAGA AGCTCTTTGC TGAGTGTCAT GTAATTAATC CAAGTAAAAA GTCTCGAACA 
CGCCAGATGA ATACAAGGTC ATCAGCACAG GATATGCCTT GTCAGATCTG CTACTTGAAC 
TACCCTAACT CGTATTTCAC TGGCCTTGAA TGTGGACATA AGTTTTGTAT GCAGTGCTGG 
AGTGAATATT TAACTACCAA AATAATGGAA GAAGGCATGG GTCAGACTAT TTCGTGTCCT 
GCTCATGGTT GTGATATCTT AGTGGATGAC AACACAGTTA TGCGCCTGAT CACAGATTCA 
AAAGTTAAAT TAAAGTATCA GCATTTAATA ACAAATAGCT TTGTAGAGTG CAATCGACTG 
TTAAAGTGGT GTCCTGCCCC AGATTGCCAC CATGTTGTTA AAGTCCAATA TCCTGATGCT 
AAACCTGTTC GCTGCAAATG TGGGCGCCAA TTTTGCTTTA ACTGTGGAGA AAATTGGCAT 
GATCCTGTTA AATGTAAGTG GTTAAAGAAA TGGATTAAAA AGTGTGATGA TGACAGTGAA 
ACCTCCAATT GGATTGCAGC CAACACAAAG GAATGTCCCA AATGCCATGT CACAATTGAG 
AAGGATGGTG GTTGTAATCA CATGGTCTGT CGTAACCAGA ATTGTAAAGC AGAGTTTTGC 
TGGGTGTGTC TTGGCCCATG GGAACCACAT GGATCTGCCT GGTACAACTG TAACCGCTAT 
AATGAGGATG ATGCAAAGGC AGCAAGAGAT GCACAGGAGC GATCTAGGGC AGCCCTGCAG 
AGGTACCTGT TCTACTGTAA TCGCTATATG AACCACATGC AGAGCCTGCG CTTTGAGCAC 
AAACTATATG CTCAGGTGAA ACAGAAAATG GAGGAGATGC AGCAGCACAA CATGTCCTGG 
ATTGAGGTGC AGTTCCTGAA GAAGGCAGTT GATGTCCTCT GCCAGTGTCG TGCCACACTC 
ATGTACACTT ATGTCTTCGC TTTCTACCTC AAAAAGAATA ACCAGTCCAT TATCTTTGAG 
AATAACCAAG CAGATCTAGA GAATGCCACA GAGGTGCTCT CGGGCTACCT TGAACGAGAT 
ATTTCCCAAG ATTCTCTGCA GGATATAAAG CAGAAAGTAC AAGACAAGTA CAGATACTGT 
GAGAGTCGAC GAAGGGTTTT GTTACAGCAT GTGCATGAAG GCTATGAAAA AGATCTGTGG 
GAGTACATTG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARIH1 ( XM_011521439.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519741.1 Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), transcript variant X2, mRNA.

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CloneID	OHu66508
Clone ID Related Accession (Same CDS sequence)	XM_011521439.3 , XM_011521439.2
Accession Version	XM_011521439.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase ARIH1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCCGG GAGGTCAACG AGGTCATCCA GTGCATATTG GGTACTTCCA GAAGTATGAA 
TTCAGTGATG CAAAGGACAG GCGGGCTGCA GCAGCAAAGA ATCCAGCAAC TATCACAAGA 
ATACTCCTTA GCCACTTCAA TTGGGATAAA GAGAAGCTAA TGGAAAGGTA CTTTGATGGA 
AACCTGGAGA AGCTCTTTGC TGAGTGTCAT GTAATTAATC CAAGTAAAAA GTCTCGAACA 
CGCCAGATGA ATACAAGGTC ATCAGCACAG GATATGCCTT GTCAGATCTG CTACTTGAAC 
TACCCTAACT CGTATTTCAC TGGCCTTGAA TGTGGACATA AGTTTTGTAT GCAGTGCTGG 
AGTGAATATT TAACTACCAA AATAATGGAA GAAGGCATGG GTCAGACTAT TTCGTGTCCT 
GCTCATGGTT GTGATATCTT AGTGGATGAC AACACAGTTA TGCGCCTGAT CACAGATTCA 
AAAGTTAAAT TAAAGTATCA GCATTTAATA ACAAATAGCT TTGTAGAGTG CAATCGACTG 
TTAAAGTGGT GTCCTGCCCC AGATTGCCAC CATGTTGTTA AAGTCCAATA TCCTGATGCT 
AAACCTGTTC GCTGCAAATG TGGGCGCCAA TTTTGCTTTA ACTGTGGAGA AAATTGGCAT 
GATCCTGTTA AATGTAAGTG GTTAAAGAAA TGGATTAAAA AGTGTGATGA TGACAGTGAA 
ACCTCCAATT GGATTGCAGC CAACACAAAG GAATGTCCCA AATGCCATGT CACAATTGAG 
AAGGATGGTG GTTGTAATCA CATGGTCTGT CGTAACCAGA ATTGTAAAGC AGAGTTTTGC 
TGGGTGTGTC TTGGCCCATG GGAACCACAT GGATCTGCCT GGTACAACTG TAACCGCTAT 
AATGAGGATG ATGCAAAGGC AGCAAGAGAT GCACAGGAGC GATCTAGGGC AGCCCTGCAG 
AGGTACCTGT TCTACTGTAA TCGCTATATG AACCACATGC AGAGCCTGCG CTTTGAGCAC 
AAACTATATG CTCAGGTGAA ACAGAAAATG GAGGAGATGC AGCAGCACAA CATGTCCTGG 
ATTGAGGTGC AGTTCCTGAA GAAGGCAGTT GATGTCCTCT GCCAGTGTCG TGCCACACTC 
ATGTACACTT ATGTCTTCGC TTTCTACCTC AAAAAGAATA ACCAGTCCAT TATCTTTGAG 
AATAACCAAG CAGATCTAGA GAATGCCACA GAGGTGCTCT CGGGCTACCT TGAACGAGAT 
ATTTCCCAAG ATTCTCTGCA GGATATAAAG CAGAAAGTAC AAGACAAGTA CAGATACTGT 
GAGAGTCGAC GAAGGGTTTT GTTACAGCAT GTGCATGAAG GCTATGAAAA AGATCTGTGG 
GAGTACATTG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519741.1
CDS	105..1502
Translation	MYPGGQRGHPVHIGYFQKYEFSDAKDRRAAAAKNPATITRILLSHFNWDKEKLMERYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIED

Target ORF information:

RefSeq Version	XM_011521439.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521439.2

ORF Insert Sequence:

ATGTATCCGG GAGGTCAACG AGGTCATCCA GTGCATATTG GGTACTTCCA GAAGTATGAA 
TTCAGTGATG CAAAGGACAG GCGGGCTGCA GCAGCAAAGA ATCCAGCAAC TATCACAAGA 
ATACTCCTTA GCCACTTCAA TTGGGATAAA GAGAAGCTAA TGGAAAGGTA CTTTGATGGA 
AACCTGGAGA AGCTCTTTGC TGAGTGTCAT GTAATTAATC CAAGTAAAAA GTCTCGAACA 
CGCCAGATGA ATACAAGGTC ATCAGCACAG GATATGCCTT GTCAGATCTG CTACTTGAAC 
TACCCTAACT CGTATTTCAC TGGCCTTGAA TGTGGACATA AGTTTTGTAT GCAGTGCTGG 
AGTGAATATT TAACTACCAA AATAATGGAA GAAGGCATGG GTCAGACTAT TTCGTGTCCT 
GCTCATGGTT GTGATATCTT AGTGGATGAC AACACAGTTA TGCGCCTGAT CACAGATTCA 
AAAGTTAAAT TAAAGTATCA GCATTTAATA ACAAATAGCT TTGTAGAGTG CAATCGACTG 
TTAAAGTGGT GTCCTGCCCC AGATTGCCAC CATGTTGTTA AAGTCCAATA TCCTGATGCT 
AAACCTGTTC GCTGCAAATG TGGGCGCCAA TTTTGCTTTA ACTGTGGAGA AAATTGGCAT 
GATCCTGTTA AATGTAAGTG GTTAAAGAAA TGGATTAAAA AGTGTGATGA TGACAGTGAA 
ACCTCCAATT GGATTGCAGC CAACACAAAG GAATGTCCCA AATGCCATGT CACAATTGAG 
AAGGATGGTG GTTGTAATCA CATGGTCTGT CGTAACCAGA ATTGTAAAGC AGAGTTTTGC 
TGGGTGTGTC TTGGCCCATG GGAACCACAT GGATCTGCCT GGTACAACTG TAACCGCTAT 
AATGAGGATG ATGCAAAGGC AGCAAGAGAT GCACAGGAGC GATCTAGGGC AGCCCTGCAG 
AGGTACCTGT TCTACTGTAA TCGCTATATG AACCACATGC AGAGCCTGCG CTTTGAGCAC 
AAACTATATG CTCAGGTGAA ACAGAAAATG GAGGAGATGC AGCAGCACAA CATGTCCTGG 
ATTGAGGTGC AGTTCCTGAA GAAGGCAGTT GATGTCCTCT GCCAGTGTCG TGCCACACTC 
ATGTACACTT ATGTCTTCGC TTTCTACCTC AAAAAGAATA ACCAGTCCAT TATCTTTGAG 
AATAACCAAG CAGATCTAGA GAATGCCACA GAGGTGCTCT CGGGCTACCT TGAACGAGAT 
ATTTCCCAAG ATTCTCTGCA GGATATAAAG CAGAAAGTAC AAGACAAGTA CAGATACTGT 
GAGAGTCGAC GAAGGGTTTT GTTACAGCAT GTGCATGAAG GCTATGAAAA AGATCTGTGG 
GAGTACATTG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARIH1 ( XM_011521438.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519740.1 Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu66507
Clone ID Related Accession (Same CDS sequence)	XM_011521438.2 , XM_011521438.3
Accession Version	XM_011521438.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase ARIH1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521438.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATACCCT GGATCTGGGC GAGGTGGAGC TGGTGGAGCC CGGGCTGGGC GTCGGCGGGG 
AGCGGGACGG ACTGCTGTGC GGGGAGACGG GCGGTGGCGG CGGCAGCGCT CTGGGGCCCG 
GCGGTGGCGG CGGCGGCGGC GGCGGCGGTG GTGGTGGCGG GCCGGGGCAT GAGCAGGAGG 
AGGATTACCG CTACGAGGTG CTCACGGCCG AGCAGATTCT ACAACACATG GTGGAATGTA 
TCCGGGAGGT CAACGAGGTC ATCCAGGTAC TTTGATGGAA ACCTGGAGAA GCTCTTTGCT 
GAGTGTCATG TAATTAATCC AAGTAAAAAG TCTCGAACAC GCCAGATGAA TACAAGGTCA 
TCAGCACAGG ATATGCCTTG TCAGATCTGC TACTTGAACT ACCCTAACTC GTATTTCACT 
GGCCTTGAAT GTGGACATAA GTTTTGTATG CAGTGCTGGA GTGAATATTT AACTACCAAA 
ATAATGGAAG AAGGCATGGG TCAGACTATT TCGTGTCCTG CTCATGGTTG TGATATCTTA 
GTGGATGACA ACACAGTTAT GCGCCTGATC ACAGATTCAA AAGTTAAATT AAAGTATCAG 
CATTTAATAA CAAATAGCTT TGTAGAGTGC AATCGACTGT TAAAGTGGTG TCCTGCCCCA 
GATTGCCACC ATGTTGTTAA AGTCCAATAT CCTGATGCTA AACCTGTTCG CTGCAAATGT 
GGGCGCCAAT TTTGCTTTAA CTGTGGAGAA AATTGGCATG ATCCTGTTAA ATGTAAGTGG 
TTAAAGAAAT GGATTAAAAA GTGTGATGAT GACAGTGAAA CCTCCAATTG GATTGCAGCC 
AACACAAAGG AATGTCCCAA ATGCCATGTC ACAATTGAGA AGGATGGTGG TTGTAATCAC 
ATGGTCTGTC GTAACCAGAA TTGTAAAGCA GAGTTTTGCT GGGTGTGTCT TGGCCCATGG 
GAACCACATG GATCTGCCTG GTACAACTGT AACCGCTATA ATGAGGATGA TGCAAAGGCA 
GCAAGAGATG CACAGGAGCG ATCTAGGGCA GCCCTGCAGA GGTACCTGTT CTACTGTAAT 
CGCTATATGA ACCACATGCA GAGCCTGCGC TTTGAGCACA AACTATATGC TCAGGTGAAA 
CAGAAAATGG AGGAGATGCA GCAGCACAAC ATGTCCTGGA TTGAGGTGCA GTTCCTGAAG 
AAGGCAGTTG ATGTCCTCTG CCAGTGTCGT GCCACACTCA TGTACACTTA TGTCTTCGCT 
TTCTACCTCA AAAAGAATAA CCAGTCCATT ATCTTTGAGA ATAACCAAGC AGATCTAGAG 
AATGCCACAG AGGTGCTCTC GGGCTACCTT GAACGAGATA TTTCCCAAGA TTCTCTGCAG 
GATATAAAGC AGAAAGTACA AGACAAGTAC AGATACTGTG AGAGTCGACG AAGGGTTTTG 
TTACAGCATG TGCATGAAGG CTATGAAAAA GATCTGTGGG AGTACATTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519740.1
CDS	468..1964
Translation	MIPWIWARWSWWSPGWASAGSGTDCCAGRRAVAAAALWGPAVAAAAAAAVVVAGRGMSRRRITATRCSRPSRFYNTWWNVSGRSTRSSRYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIED

Target ORF information:

RefSeq Version	XM_011521438.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521438.2

ORF Insert Sequence:

ATGATACCCT GGATCTGGGC GAGGTGGAGC TGGTGGAGCC CGGGCTGGGC GTCGGCGGGG 
AGCGGGACGG ACTGCTGTGC GGGGAGACGG GCGGTGGCGG CGGCAGCGCT CTGGGGCCCG 
GCGGTGGCGG CGGCGGCGGC GGCGGCGGTG GTGGTGGCGG GCCGGGGCAT GAGCAGGAGG 
AGGATTACCG CTACGAGGTG CTCACGGCCG AGCAGATTCT ACAACACATG GTGGAATGTA 
TCCGGGAGGT CAACGAGGTC ATCCAGGTAC TTTGATGGAA ACCTGGAGAA GCTCTTTGCT 
GAGTGTCATG TAATTAATCC AAGTAAAAAG TCTCGAACAC GCCAGATGAA TACAAGGTCA 
TCAGCACAGG ATATGCCTTG TCAGATCTGC TACTTGAACT ACCCTAACTC GTATTTCACT 
GGCCTTGAAT GTGGACATAA GTTTTGTATG CAGTGCTGGA GTGAATATTT AACTACCAAA 
ATAATGGAAG AAGGCATGGG TCAGACTATT TCGTGTCCTG CTCATGGTTG TGATATCTTA 
GTGGATGACA ACACAGTTAT GCGCCTGATC ACAGATTCAA AAGTTAAATT AAAGTATCAG 
CATTTAATAA CAAATAGCTT TGTAGAGTGC AATCGACTGT TAAAGTGGTG TCCTGCCCCA 
GATTGCCACC ATGTTGTTAA AGTCCAATAT CCTGATGCTA AACCTGTTCG CTGCAAATGT 
GGGCGCCAAT TTTGCTTTAA CTGTGGAGAA AATTGGCATG ATCCTGTTAA ATGTAAGTGG 
TTAAAGAAAT GGATTAAAAA GTGTGATGAT GACAGTGAAA CCTCCAATTG GATTGCAGCC 
AACACAAAGG AATGTCCCAA ATGCCATGTC ACAATTGAGA AGGATGGTGG TTGTAATCAC 
ATGGTCTGTC GTAACCAGAA TTGTAAAGCA GAGTTTTGCT GGGTGTGTCT TGGCCCATGG 
GAACCACATG GATCTGCCTG GTACAACTGT AACCGCTATA ATGAGGATGA TGCAAAGGCA 
GCAAGAGATG CACAGGAGCG ATCTAGGGCA GCCCTGCAGA GGTACCTGTT CTACTGTAAT 
CGCTATATGA ACCACATGCA GAGCCTGCGC TTTGAGCACA AACTATATGC TCAGGTGAAA 
CAGAAAATGG AGGAGATGCA GCAGCACAAC ATGTCCTGGA TTGAGGTGCA GTTCCTGAAG 
AAGGCAGTTG ATGTCCTCTG CCAGTGTCGT GCCACACTCA TGTACACTTA TGTCTTCGCT 
TTCTACCTCA AAAAGAATAA CCAGTCCATT ATCTTTGAGA ATAACCAAGC AGATCTAGAG 
AATGCCACAG AGGTGCTCTC GGGCTACCTT GAACGAGATA TTTCCCAAGA TTCTCTGCAG 
GATATAAAGC AGAAAGTACA AGACAAGTAC AGATACTGTG AGAGTCGACG AAGGGTTTTG 
TTACAGCATG TGCATGAAGG CTATGAAAAA GATCTGTGGG AGTACATTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARIH1 ( XM_011521438.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519740.1 Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), transcript variant X1, mRNA.

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CloneID	OHu66507
Clone ID Related Accession (Same CDS sequence)	XM_011521438.2 , XM_011521438.3
Accession Version	XM_011521438.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase ARIH1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521438.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATACCCT GGATCTGGGC GAGGTGGAGC TGGTGGAGCC CGGGCTGGGC GTCGGCGGGG 
AGCGGGACGG ACTGCTGTGC GGGGAGACGG GCGGTGGCGG CGGCAGCGCT CTGGGGCCCG 
GCGGTGGCGG CGGCGGCGGC GGCGGCGGTG GTGGTGGCGG GCCGGGGCAT GAGCAGGAGG 
AGGATTACCG CTACGAGGTG CTCACGGCCG AGCAGATTCT ACAACACATG GTGGAATGTA 
TCCGGGAGGT CAACGAGGTC ATCCAGGTAC TTTGATGGAA ACCTGGAGAA GCTCTTTGCT 
GAGTGTCATG TAATTAATCC AAGTAAAAAG TCTCGAACAC GCCAGATGAA TACAAGGTCA 
TCAGCACAGG ATATGCCTTG TCAGATCTGC TACTTGAACT ACCCTAACTC GTATTTCACT 
GGCCTTGAAT GTGGACATAA GTTTTGTATG CAGTGCTGGA GTGAATATTT AACTACCAAA 
ATAATGGAAG AAGGCATGGG TCAGACTATT TCGTGTCCTG CTCATGGTTG TGATATCTTA 
GTGGATGACA ACACAGTTAT GCGCCTGATC ACAGATTCAA AAGTTAAATT AAAGTATCAG 
CATTTAATAA CAAATAGCTT TGTAGAGTGC AATCGACTGT TAAAGTGGTG TCCTGCCCCA 
GATTGCCACC ATGTTGTTAA AGTCCAATAT CCTGATGCTA AACCTGTTCG CTGCAAATGT 
GGGCGCCAAT TTTGCTTTAA CTGTGGAGAA AATTGGCATG ATCCTGTTAA ATGTAAGTGG 
TTAAAGAAAT GGATTAAAAA GTGTGATGAT GACAGTGAAA CCTCCAATTG GATTGCAGCC 
AACACAAAGG AATGTCCCAA ATGCCATGTC ACAATTGAGA AGGATGGTGG TTGTAATCAC 
ATGGTCTGTC GTAACCAGAA TTGTAAAGCA GAGTTTTGCT GGGTGTGTCT TGGCCCATGG 
GAACCACATG GATCTGCCTG GTACAACTGT AACCGCTATA ATGAGGATGA TGCAAAGGCA 
GCAAGAGATG CACAGGAGCG ATCTAGGGCA GCCCTGCAGA GGTACCTGTT CTACTGTAAT 
CGCTATATGA ACCACATGCA GAGCCTGCGC TTTGAGCACA AACTATATGC TCAGGTGAAA 
CAGAAAATGG AGGAGATGCA GCAGCACAAC ATGTCCTGGA TTGAGGTGCA GTTCCTGAAG 
AAGGCAGTTG ATGTCCTCTG CCAGTGTCGT GCCACACTCA TGTACACTTA TGTCTTCGCT 
TTCTACCTCA AAAAGAATAA CCAGTCCATT ATCTTTGAGA ATAACCAAGC AGATCTAGAG 
AATGCCACAG AGGTGCTCTC GGGCTACCTT GAACGAGATA TTTCCCAAGA TTCTCTGCAG 
GATATAAAGC AGAAAGTACA AGACAAGTAC AGATACTGTG AGAGTCGACG AAGGGTTTTG 
TTACAGCATG TGCATGAAGG CTATGAAAAA GATCTGTGGG AGTACATTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519740.1
CDS	422..1918
Translation	MIPWIWARWSWWSPGWASAGSGTDCCAGRRAVAAAALWGPAVAAAAAAAVVVAGRGMSRRRITATRCSRPSRFYNTWWNVSGRSTRSSRYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQDMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYCESRRRVLLQHVHEGYEKDLWEYIED

Target ORF information:

RefSeq Version	XM_011521438.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ariadne RBR E3 ubiquitin protein ligase 1 (ARIH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521438.3

ORF Insert Sequence:

ATGATACCCT GGATCTGGGC GAGGTGGAGC TGGTGGAGCC CGGGCTGGGC GTCGGCGGGG 
AGCGGGACGG ACTGCTGTGC GGGGAGACGG GCGGTGGCGG CGGCAGCGCT CTGGGGCCCG 
GCGGTGGCGG CGGCGGCGGC GGCGGCGGTG GTGGTGGCGG GCCGGGGCAT GAGCAGGAGG 
AGGATTACCG CTACGAGGTG CTCACGGCCG AGCAGATTCT ACAACACATG GTGGAATGTA 
TCCGGGAGGT CAACGAGGTC ATCCAGGTAC TTTGATGGAA ACCTGGAGAA GCTCTTTGCT 
GAGTGTCATG TAATTAATCC AAGTAAAAAG TCTCGAACAC GCCAGATGAA TACAAGGTCA 
TCAGCACAGG ATATGCCTTG TCAGATCTGC TACTTGAACT ACCCTAACTC GTATTTCACT 
GGCCTTGAAT GTGGACATAA GTTTTGTATG CAGTGCTGGA GTGAATATTT AACTACCAAA 
ATAATGGAAG AAGGCATGGG TCAGACTATT TCGTGTCCTG CTCATGGTTG TGATATCTTA 
GTGGATGACA ACACAGTTAT GCGCCTGATC ACAGATTCAA AAGTTAAATT AAAGTATCAG 
CATTTAATAA CAAATAGCTT TGTAGAGTGC AATCGACTGT TAAAGTGGTG TCCTGCCCCA 
GATTGCCACC ATGTTGTTAA AGTCCAATAT CCTGATGCTA AACCTGTTCG CTGCAAATGT 
GGGCGCCAAT TTTGCTTTAA CTGTGGAGAA AATTGGCATG ATCCTGTTAA ATGTAAGTGG 
TTAAAGAAAT GGATTAAAAA GTGTGATGAT GACAGTGAAA CCTCCAATTG GATTGCAGCC 
AACACAAAGG AATGTCCCAA ATGCCATGTC ACAATTGAGA AGGATGGTGG TTGTAATCAC 
ATGGTCTGTC GTAACCAGAA TTGTAAAGCA GAGTTTTGCT GGGTGTGTCT TGGCCCATGG 
GAACCACATG GATCTGCCTG GTACAACTGT AACCGCTATA ATGAGGATGA TGCAAAGGCA 
GCAAGAGATG CACAGGAGCG ATCTAGGGCA GCCCTGCAGA GGTACCTGTT CTACTGTAAT 
CGCTATATGA ACCACATGCA GAGCCTGCGC TTTGAGCACA AACTATATGC TCAGGTGAAA 
CAGAAAATGG AGGAGATGCA GCAGCACAAC ATGTCCTGGA TTGAGGTGCA GTTCCTGAAG 
AAGGCAGTTG ATGTCCTCTG CCAGTGTCGT GCCACACTCA TGTACACTTA TGTCTTCGCT 
TTCTACCTCA AAAAGAATAA CCAGTCCATT ATCTTTGAGA ATAACCAAGC AGATCTAGAG 
AATGCCACAG AGGTGCTCTC GGGCTACCTT GAACGAGATA TTTCCCAAGA TTCTCTGCAG 
GATATAAAGC AGAAAGTACA AGACAAGTAC AGATACTGTG AGAGTCGACG AAGGGTTTTG 
TTACAGCATG TGCATGAAGG CTATGAAAAA GATCTGTGGG AGTACATTGA GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Parkin-Independent Mitophagy Controls Chemotherapeutic Response in Cancer Cells.
Cell reports20(12)2846-2859(2017 Sep)
Villa E,Pro?cs E,Rubio-Pati?o C,Obba S,Zunino B,Bossowski JP,Rozier RM,Chiche J,Mondrag?n L,Riley JS,Marchetti S,Verhoeyen E,Tait SWG,Ricci JE

Structural insights into the mechanism and E2 specificity of the RBR E3 ubiquitin ligase HHARI.
Nature communications8(1)211(2017 08)
Yuan L,Lv Z,Atkison JH,Olsen SK

Structural Studies of HHARI/UbcH7?Ub Reveal Unique E2?Ub Conformational Restriction by RBR RING1.
Structure (London, England : 1993)25(6)890-900.e5(2017 Jun)
Dove KK,Olszewski JL,Martino L,Duda DM,Wu XS,Miller DJ,Reiter KH,Rittinger K,Schulman BA,Klevit RE

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ARIH1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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