ALPI cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALPI
Entrez Gene ID	248
Full Name	alkaline phosphatase, intestinal
Synonyms	IAP
General protein information	Preferred Names alkaline phosphatase, intestinal Names intestinal-type alkaline phosphatase Kasahara isozyme alkaline phosphomonoesterase glycerophosphatase intestinal alkaline phosphatase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q37.1
Summary	There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. [provided by RefSeq, Dec 2014].
Disorder MIM:	171740

mRNA and Protein(s)

mRNA	Protein	Name
NM_001631.4	NP_001622.2	intestinal-type alkaline phosphatase precursor
NM_001631.5	NP_001622.2	intestinal-type alkaline phosphatase precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ALPP	XP_534605.5
Bos taurus (cattle)	LOC528262	XP_002685648.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011302	XP_309345.4
Homo sapiens (human)	ALPI	NP_001622.2
Pan troglodytes (chimpanzee)	ALPI	XP_003309566.1
Mus musculus (house mouse)	Akp3	NP_031458.2
Rattus norvegicus (Norway rat)	Akp3	NP_073171.1
Bos taurus (cattle)	LOC100125266	NP_001096792.1
Gallus gallus (chicken)	ALPP	XP_003641809.1
Danio rerio (zebrafish)	alpi.2	NP_001020359.1
Drosophila melanogaster (fruit fly)	CG5150	NP_647977.1
Drosophila melanogaster (fruit fly)	CG10827	NP_650931.1
Bos taurus (cattle)	ALPI	XP_005203102.1
Bos taurus (cattle)	LOC516378	XP_002685647.1
Xenopus tropicalis (tropical clawed frog)	alpi.1	XP_002937387.2

Gene Ontology
Bibliography

Related articles in PubMed

1-alpha,25-Dihydroxyvitamin D
Noda S, Yamada A, Nakaoka K, Goseki-Sone M
Nutrition research (New York, N.Y.)4659-67(2017 Oct)

Menaquinone-4 (vitamin K
Noda S, Yamada A, Tanabe R, Nakaoka K, Hosoi T, Goseki-Sone M
Nutrition research (New York, N.Y.)36(11)1269-1276(2016 Nov)

Intestinal Alkaline Phosphatase Regulates Tight Junction Protein Levels.
Liu W, Hu D, Huo H, Zhang W, Adiliaghdam F, Morrison S, Ramirez JM, Gul SS, Hamarneh SR, Hodin RA
Journal of the American College of Surgeons222(6)1009-17(2016 Jun)

Intestinal alkaline phosphatase: a summary of its role in clinical disease.
Fawley J, Gourlay DM
The Journal of surgical research202(1)225-34(2016 May)

Alkaline Phosphatase, Soluble Extracellular Adenine Nucleotides, and Adenosine Production after Infant Cardiopulmonary Bypass.
Davidson JA, Urban T, Tong S, Twite M, Woodruff A, Wischmeyer PE, Klawitter J
PloS one11(7)e0158981(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Intestinal alkaline phosphatase is a major regulator of gut mucosal permeability and appears to work at least partly through improving tight junction protein levels and localization.
Title: Intestinal Alkaline Phosphatase Regulates Tight Junction Protein Levels.

Review of the role of intestinal alkaline phosphatase in inflammatory diseases. Loss of IAP expression or function is associated with increased intestinal inflammation, dysbiosis, bacterial translocation and subsequently systemic inflammation.
Title: Intestinal alkaline phosphatase: a summary of its role in clinical disease.

Data indicate alkaline phosphatase (AP) as the primary soluble ectonucleotidase in infants undergoing cardiopulmonary bypass and show decreased capacity to clear AMP when AP activity decreases post-bypass.
Title: Alkaline Phosphatase, Soluble Extracellular Adenine Nucleotides, and Adenosine Production after Infant Cardiopulmonary Bypass.

A High Level of Intestinal Alkaline Phosphatase Is Protective Against Type 2 Diabetes Mellitus Irrespective of Obesity
Title: A High Level of Intestinal Alkaline Phosphatase Is Protective Against Type 2 Diabetes Mellitus Irrespective of Obesity.

The expression of ALPi and MUC5AC in cocultures of Caco-2 and HT29 cells developed for permeability studies is reported.
Title: Optimization of Caco-2 and HT29 co-culture in vitro cell models for permeability studies.

The following ALPI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALPI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25845	NM_001631.5 Latest version!	Homo sapiens alkaline phosphatase, intestinal (ALPI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
View Old Accession Versions >>
OHu25845	NM_001631.4	Homo sapiens alkaline phosphatase, intestinal (ALPI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALPI ( NM_001631.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001622.2 Homo sapiens alkaline phosphatase, intestinal (ALPI), mRNA.

Price & Availability↑

CloneID	OHu25845
Clone ID Related Accession (Same CDS sequence)	NM_001631.4 , NM_001631.5
Accession Version	NM_001631.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Homo sapiens(human)
Product	intestinal-type alkaline phosphatase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA376676.1, BC132678.1 and AC068134.5. On Dec 30, 2014 this sequence version replaced NM_001631.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC132678.1, AH002601.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCAGGGGC CCTGGGTGCT GCTGCTGCTG GGCCTGAGGC TACAGCTCTC CCTGGGCGTC 
ATCCCAGCTG AGGAGGAGAA CCCGGCCTTC TGGAACCGCC AGGCAGCTGA GGCCCTGGAT 
GCTGCCAAGA AGCTGCAGCC CATCCAGAAG GTCGCCAAGA ACCTCATCCT CTTCCTGGGC 
GATGGGTTGG GGGTGCCCAC GGTGACAGCC ACCAGGATCC TAAAGGGGCA GAAGAATGGC 
AAACTGGGGC CTGAGACGCC CCTGGCCATG GACCGCTTCC CATACCTGGC TCTGTCCAAG 
ACATACAATG TGGACAGACA GGTGCCAGAC AGCGCAGCCA CAGCCACGGC CTACCTGTGC 
GGGGTCAAGG CCAACTTCCA GACCATCGGC TTGAGTGCAG CCGCCCGCTT TAACCAGTGC 
AACACGACAC GCGGCAATGA GGTCATCTCC GTGATGAACC GGGCCAAGCA AGCAGGAAAG 
TCAGTAGGAG TGGTGACCAC CACACGGGTG CAGCACGCCT CGCCAGCCGG CACCTACGCA 
CACACAGTGA ACCGCAACTG GTACTCAGAT GCTGACATGC CTGCCTCAGC CCGCCAGGAG 
GGGTGCCAGG ACATCGCCAC TCAGCTCATC TCCAACATGG ACATTGACGT GATCCTTGGC 
GGAGGCCGCA AGTACATGTT TCCCATGGGG ACCCCAGACC CTGAGTACCC AGCTGATGCC 
AGCCAGAATG GAATCAGGCT GGACGGGAAG AACCTGGTGC AGGAATGGCT GGCAAAGCAC 
CAGGGTGCCT GGTATGTGTG GAACCGCACT GAGCTCATGC AGGCGTCCCT GGACCAGTCT 
GTGACCCATC TCATGGGCCT CTTTGAGCCC GGAGACACGA AATATGAGAT CCACCGAGAC 
CCCACACTGG ACCCCTCCCT GATGGAGATG ACAGAGGCTG CCCTGCGCCT GCTGAGCAGG 
AACCCCCGCG GCTTCTACCT CTTTGTGGAG GGCGGCCGCA TCGACCATGG TCATCATGAG 
GGTGTGGCTT ACCAGGCACT CACTGAGGCG GTCATGTTCG ACGACGCCAT TGAGAGGGCG 
GGCCAGCTCA CCAGCGAGGA GGACACGCTG ACCCTCGTCA CCGCTGACCA CTCCCATGTC 
TTCTCCTTTG GTGGCTACAC CTTGCGAGGG AGCTCCATCT TCGGGTTGGC CCCCAGCAAG 
GCTCAGGACA GCAAAGCCTA CACGTCCATC CTGTACGGCA ATGGCCCGGG CTACGTGTTC 
AACTCAGGCG TGCGACCAGA CGTGAATGAG AGCGAGAGCG GGAGCCCCGA TTACCAGCAG 
CAGGCGGCGG TGCCCCTGTC GTCCGAGACC CACGGAGGCG AAGACGTGGC GGTGTTTGCG 
CGCGGCCCGC AGGCGCACCT GGTGCATGGT GTGCAGGAGC AGAGCTTCGT AGCGCATGTC 
ATGGCCTTCG CTGCCTGTCT GGAGCCCTAC ACGGCCTGCG ACCTGGCGCC TCCCGCCTGC 
ACCACCGACG CCGCGCACCC AGTTGCCGCG TCGCTGCCAC TGCTGGCCGG GACCCTGCTG 
CTGCTGGGGG CGTCCGCTGC TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001622.2
CDS	89..1675
Translation	MQGPWVLLLLGLRLQLSLGVIPAEEENPAFWNRQAAEALDAAKKLQPIQKVAKNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSAATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGDTKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQALTEAVMFDDAIERAGQLTSEEDTLTLVTADHSHVFSFGGYTLRGSSIFGLAPSKAQDSKAYTSILYGNGPGYVFNSGVRPDVNESESGSPDYQQQAAVPLSSETHGGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPACTTDAAHPVAASLPLLAGTLLLLGASAAP

Target ORF information:

RefSeq Version	NM_001631.4
Organism	Homo sapiens(human)
Definition	Homo sapiens alkaline phosphatase, intestinal (ALPI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001631.4

ORF Insert Sequence:

ATGCAGGGGC CCTGGGTGCT GCTGCTGCTG GGCCTGAGGC TACAGCTCTC CCTGGGCGTC 
ATCCCAGCTG AGGAGGAGAA CCCGGCCTTC TGGAACCGCC AGGCAGCTGA GGCCCTGGAT 
GCTGCCAAGA AGCTGCAGCC CATCCAGAAG GTCGCCAAGA ACCTCATCCT CTTCCTGGGC 
GATGGGTTGG GGGTGCCCAC GGTGACAGCC ACCAGGATCC TAAAGGGGCA GAAGAATGGC 
AAACTGGGGC CTGAGACGCC CCTGGCCATG GACCGCTTCC CATACCTGGC TCTGTCCAAG 
ACATACAATG TGGACAGACA GGTGCCAGAC AGCGCAGCCA CAGCCACGGC CTACCTGTGC 
GGGGTCAAGG CCAACTTCCA GACCATCGGC TTGAGTGCAG CCGCCCGCTT TAACCAGTGC 
AACACGACAC GCGGCAATGA GGTCATCTCC GTGATGAACC GGGCCAAGCA AGCAGGAAAG 
TCAGTAGGAG TGGTGACCAC CACACGGGTG CAGCACGCCT CGCCAGCCGG CACCTACGCA 
CACACAGTGA ACCGCAACTG GTACTCAGAT GCTGACATGC CTGCCTCAGC CCGCCAGGAG 
GGGTGCCAGG ACATCGCCAC TCAGCTCATC TCCAACATGG ACATTGACGT GATCCTTGGC 
GGAGGCCGCA AGTACATGTT TCCCATGGGG ACCCCAGACC CTGAGTACCC AGCTGATGCC 
AGCCAGAATG GAATCAGGCT GGACGGGAAG AACCTGGTGC AGGAATGGCT GGCAAAGCAC 
CAGGGTGCCT GGTATGTGTG GAACCGCACT GAGCTCATGC AGGCGTCCCT GGACCAGTCT 
GTGACCCATC TCATGGGCCT CTTTGAGCCC GGAGACACGA AATATGAGAT CCACCGAGAC 
CCCACACTGG ACCCCTCCCT GATGGAGATG ACAGAGGCTG CCCTGCGCCT GCTGAGCAGG 
AACCCCCGCG GCTTCTACCT CTTTGTGGAG GGCGGCCGCA TCGACCATGG TCATCATGAG 
GGTGTGGCTT ACCAGGCACT CACTGAGGCG GTCATGTTCG ACGACGCCAT TGAGAGGGCG 
GGCCAGCTCA CCAGCGAGGA GGACACGCTG ACCCTCGTCA CCGCTGACCA CTCCCATGTC 
TTCTCCTTTG GTGGCTACAC CTTGCGAGGG AGCTCCATCT TCGGGTTGGC CCCCAGCAAG 
GCTCAGGACA GCAAAGCCTA CACGTCCATC CTGTACGGCA ATGGCCCGGG CTACGTGTTC 
AACTCAGGCG TGCGACCAGA CGTGAATGAG AGCGAGAGCG GGAGCCCCGA TTACCAGCAG 
CAGGCGGCGG TGCCCCTGTC GTCCGAGACC CACGGAGGCG AAGACGTGGC GGTGTTTGCG 
CGCGGCCCGC AGGCGCACCT GGTGCATGGT GTGCAGGAGC AGAGCTTCGT AGCGCATGTC 
ATGGCCTTCG CTGCCTGTCT GGAGCCCTAC ACGGCCTGCG ACCTGGCGCC TCCCGCCTGC 
ACCACCGACG CCGCGCACCC AGTTGCCGCG TCGCTGCCAC TGCTGGCCGG GACCCTGCTG 
CTGCTGGGGG CGTCCGCTGC TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALPI ( NM_001631.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001622.2 Homo sapiens alkaline phosphatase, intestinal (ALPI), mRNA.

Price & Availability↑

CloneID	OHu25845
Clone ID Related Accession (Same CDS sequence)	NM_001631.4 , NM_001631.5
Accession Version	NM_001631.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-14
Organism	Homo sapiens(human)
Product	intestinal-type alkaline phosphatase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA376676.1, BC132678.1 and AC068134.5. On Nov 29, 2018 this sequence version replaced NM_001631.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC132678.1, AH002601.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000295463.4/ ENSP00000295463.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGGGGC CCTGGGTGCT GCTGCTGCTG GGCCTGAGGC TACAGCTCTC CCTGGGCGTC 
ATCCCAGCTG AGGAGGAGAA CCCGGCCTTC TGGAACCGCC AGGCAGCTGA GGCCCTGGAT 
GCTGCCAAGA AGCTGCAGCC CATCCAGAAG GTCGCCAAGA ACCTCATCCT CTTCCTGGGC 
GATGGGTTGG GGGTGCCCAC GGTGACAGCC ACCAGGATCC TAAAGGGGCA GAAGAATGGC 
AAACTGGGGC CTGAGACGCC CCTGGCCATG GACCGCTTCC CATACCTGGC TCTGTCCAAG 
ACATACAATG TGGACAGACA GGTGCCAGAC AGCGCAGCCA CAGCCACGGC CTACCTGTGC 
GGGGTCAAGG CCAACTTCCA GACCATCGGC TTGAGTGCAG CCGCCCGCTT TAACCAGTGC 
AACACGACAC GCGGCAATGA GGTCATCTCC GTGATGAACC GGGCCAAGCA AGCAGGAAAG 
TCAGTAGGAG TGGTGACCAC CACACGGGTG CAGCACGCCT CGCCAGCCGG CACCTACGCA 
CACACAGTGA ACCGCAACTG GTACTCAGAT GCTGACATGC CTGCCTCAGC CCGCCAGGAG 
GGGTGCCAGG ACATCGCCAC TCAGCTCATC TCCAACATGG ACATTGACGT GATCCTTGGC 
GGAGGCCGCA AGTACATGTT TCCCATGGGG ACCCCAGACC CTGAGTACCC AGCTGATGCC 
AGCCAGAATG GAATCAGGCT GGACGGGAAG AACCTGGTGC AGGAATGGCT GGCAAAGCAC 
CAGGGTGCCT GGTATGTGTG GAACCGCACT GAGCTCATGC AGGCGTCCCT GGACCAGTCT 
GTGACCCATC TCATGGGCCT CTTTGAGCCC GGAGACACGA AATATGAGAT CCACCGAGAC 
CCCACACTGG ACCCCTCCCT GATGGAGATG ACAGAGGCTG CCCTGCGCCT GCTGAGCAGG 
AACCCCCGCG GCTTCTACCT CTTTGTGGAG GGCGGCCGCA TCGACCATGG TCATCATGAG 
GGTGTGGCTT ACCAGGCACT CACTGAGGCG GTCATGTTCG ACGACGCCAT TGAGAGGGCG 
GGCCAGCTCA CCAGCGAGGA GGACACGCTG ACCCTCGTCA CCGCTGACCA CTCCCATGTC 
TTCTCCTTTG GTGGCTACAC CTTGCGAGGG AGCTCCATCT TCGGGTTGGC CCCCAGCAAG 
GCTCAGGACA GCAAAGCCTA CACGTCCATC CTGTACGGCA ATGGCCCGGG CTACGTGTTC 
AACTCAGGCG TGCGACCAGA CGTGAATGAG AGCGAGAGCG GGAGCCCCGA TTACCAGCAG 
CAGGCGGCGG TGCCCCTGTC GTCCGAGACC CACGGAGGCG AAGACGTGGC GGTGTTTGCG 
CGCGGCCCGC AGGCGCACCT GGTGCATGGT GTGCAGGAGC AGAGCTTCGT AGCGCATGTC 
ATGGCCTTCG CTGCCTGTCT GGAGCCCTAC ACGGCCTGCG ACCTGGCGCC TCCCGCCTGC 
ACCACCGACG CCGCGCACCC AGTTGCCGCG TCGCTGCCAC TGCTGGCCGG GACCCTGCTG 
CTGCTGGGGG CGTCCGCTGC TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001622.2
CDS	48..1634
Translation	MQGPWVLLLLGLRLQLSLGVIPAEEENPAFWNRQAAEALDAAKKLQPIQKVAKNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSAATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGDTKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQALTEAVMFDDAIERAGQLTSEEDTLTLVTADHSHVFSFGGYTLRGSSIFGLAPSKAQDSKAYTSILYGNGPGYVFNSGVRPDVNESESGSPDYQQQAAVPLSSETHGGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPACTTDAAHPVAASLPLLAGTLLLLGASAAP

Target ORF information:

RefSeq Version	NM_001631.5
Organism	Homo sapiens(human)
Definition	Homo sapiens alkaline phosphatase, intestinal (ALPI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001631.5

ORF Insert Sequence:

ATGCAGGGGC CCTGGGTGCT GCTGCTGCTG GGCCTGAGGC TACAGCTCTC CCTGGGCGTC 
ATCCCAGCTG AGGAGGAGAA CCCGGCCTTC TGGAACCGCC AGGCAGCTGA GGCCCTGGAT 
GCTGCCAAGA AGCTGCAGCC CATCCAGAAG GTCGCCAAGA ACCTCATCCT CTTCCTGGGC 
GATGGGTTGG GGGTGCCCAC GGTGACAGCC ACCAGGATCC TAAAGGGGCA GAAGAATGGC 
AAACTGGGGC CTGAGACGCC CCTGGCCATG GACCGCTTCC CATACCTGGC TCTGTCCAAG 
ACATACAATG TGGACAGACA GGTGCCAGAC AGCGCAGCCA CAGCCACGGC CTACCTGTGC 
GGGGTCAAGG CCAACTTCCA GACCATCGGC TTGAGTGCAG CCGCCCGCTT TAACCAGTGC 
AACACGACAC GCGGCAATGA GGTCATCTCC GTGATGAACC GGGCCAAGCA AGCAGGAAAG 
TCAGTAGGAG TGGTGACCAC CACACGGGTG CAGCACGCCT CGCCAGCCGG CACCTACGCA 
CACACAGTGA ACCGCAACTG GTACTCAGAT GCTGACATGC CTGCCTCAGC CCGCCAGGAG 
GGGTGCCAGG ACATCGCCAC TCAGCTCATC TCCAACATGG ACATTGACGT GATCCTTGGC 
GGAGGCCGCA AGTACATGTT TCCCATGGGG ACCCCAGACC CTGAGTACCC AGCTGATGCC 
AGCCAGAATG GAATCAGGCT GGACGGGAAG AACCTGGTGC AGGAATGGCT GGCAAAGCAC 
CAGGGTGCCT GGTATGTGTG GAACCGCACT GAGCTCATGC AGGCGTCCCT GGACCAGTCT 
GTGACCCATC TCATGGGCCT CTTTGAGCCC GGAGACACGA AATATGAGAT CCACCGAGAC 
CCCACACTGG ACCCCTCCCT GATGGAGATG ACAGAGGCTG CCCTGCGCCT GCTGAGCAGG 
AACCCCCGCG GCTTCTACCT CTTTGTGGAG GGCGGCCGCA TCGACCATGG TCATCATGAG 
GGTGTGGCTT ACCAGGCACT CACTGAGGCG GTCATGTTCG ACGACGCCAT TGAGAGGGCG 
GGCCAGCTCA CCAGCGAGGA GGACACGCTG ACCCTCGTCA CCGCTGACCA CTCCCATGTC 
TTCTCCTTTG GTGGCTACAC CTTGCGAGGG AGCTCCATCT TCGGGTTGGC CCCCAGCAAG 
GCTCAGGACA GCAAAGCCTA CACGTCCATC CTGTACGGCA ATGGCCCGGG CTACGTGTTC 
AACTCAGGCG TGCGACCAGA CGTGAATGAG AGCGAGAGCG GGAGCCCCGA TTACCAGCAG 
CAGGCGGCGG TGCCCCTGTC GTCCGAGACC CACGGAGGCG AAGACGTGGC GGTGTTTGCG 
CGCGGCCCGC AGGCGCACCT GGTGCATGGT GTGCAGGAGC AGAGCTTCGT AGCGCATGTC 
ATGGCCTTCG CTGCCTGTCT GGAGCCCTAC ACGGCCTGCG ACCTGGCGCC TCCCGCCTGC 
ACCACCGACG CCGCGCACCC AGTTGCCGCG TCGCTGCCAC TGCTGGCCGG GACCCTGCTG 
CTGCTGGGGG CGTCCGCTGC TCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

1-alpha,25-Dihydroxyvitamin D
Nutrition research (New York, N.Y.)4659-67(2017 Oct)
Noda S,Yamada A,Nakaoka K,Goseki-Sone M

Menaquinone-4 (vitamin K
Nutrition research (New York, N.Y.)36(11)1269-1276(2016 Nov)
Noda S,Yamada A,Tanabe R,Nakaoka K,Hosoi T,Goseki-Sone M

Intestinal Alkaline Phosphatase Regulates Tight Junction Protein Levels.
Journal of the American College of Surgeons222(6)1009-17(2016 Jun)
Liu W,Hu D,Huo H,Zhang W,Adiliaghdam F,Morrison S,Ramirez JM,Gul SS,Hamarneh SR,Hodin RA

Intestinal alkaline phosphatase: a summary of its role in clinical disease.
The Journal of surgical research202(1)225-34(2016 May)
Fawley J,Gourlay DM

Alkaline Phosphatase, Soluble Extracellular Adenine Nucleotides, and Adenosine Production after Infant Cardiopulmonary Bypass.
PloS one11(7)e0158981(2016)
Davidson JA,Urban T,Tong S,Twite M,Woodruff A,Wischmeyer PE,Klawitter J