SMG1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011545769.3	XP_011544071.1	serine/threonine-protein kinase SMG1 isoform X2
XM_011545770.1	XP_011544072.1	serine/threonine-protein kinase SMG1 isoform X6
XM_005255182.3	XP_005255239.1	serine/threonine-protein kinase SMG1 isoform X1
XM_024450199.1	XP_024305967.1	serine/threonine-protein kinase SMG1 isoform X5
XM_011545769.2	XP_011544071.1	serine/threonine-protein kinase SMG1 isoform X2
XM_017023066.1	XP_016878555.1	serine/threonine-protein kinase SMG1 isoform X3
NM_015092.5	NP_055907.3	serine/threonine-protein kinase SMG1
XM_005255184.3	XP_005255241.1	serine/threonine-protein kinase SMG1 isoform X4
NM_015092.4	NP_055907.3	serine/threonine-protein kinase SMG1
XM_005255183.4	XP_005255240.1	serine/threonine-protein kinase SMG1 isoform X3
XM_024450198.1	XP_024305966.1	serine/threonine-protein kinase SMG1 isoform X5
XM_017023067.1	XP_016878556.1	serine/threonine-protein kinase SMG1 isoform X6
XM_005255183.3	XP_005255240.1	serine/threonine-protein kinase SMG1 isoform X3
XM_017023066.2	XP_016878555.1	serine/threonine-protein kinase SMG1 isoform X3
XM_005255184.4	XP_005255241.1	serine/threonine-protein kinase SMG1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu109356	NM_015092.5 Latest version!	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu26275	NM_015092.4 Latest version!	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu104104	XM_024450198.1 Latest version!	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu104104	XM_024450199.1 Latest version!	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu49088	XM_005255182.3 Latest version!	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu49089	XM_017023066.2 Latest version!	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu49089	XM_005255183.4 Latest version!	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu49090	XM_005255184.4 Latest version!	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu65357	XM_011545769.3 Latest version!	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu65358	XM_017023067.1 Latest version!	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu65358	XM_011545770.1 Latest version!	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu65357	XM_011545769.2	Homo sapiens SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu49089	XM_005255183.3	Homo sapiens SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu49089	XM_017023066.1	Homo sapiens SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu49090	XM_005255184.3	Homo sapiens SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

SMG1 ( NM_015092.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055907.3 Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), mRNA.

Price & Availability↑

CloneID	OHu109356
Clone ID Related Accession (Same CDS sequence)	NM_015092.5
Accession Version	NM_015092.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA433527.1, AW503964.1, DA854035.1, AB061371.1, BE079340.1, AY014957.1, AL833298.1, AC138811.3, BC037335.1, AK025794.1 and AW088221.1. On Nov 22, 2019 this sequence version replaced NM_015092.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB061371.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGGGTT AACAATGTGA CCAGCCCTGA GTTCACCAGT 
GTTCAGCATG GCAGTCGTGC TTTAGCCACC AAAGACATGA GGAAATCACA GGAGAGATCG 
ATGTCTTATT CTGATGAGTC TCGACTGTCG AATCTTCTTC GGAGGATCAC CCGGGAAGAC 
GACAGAGACC GAAGATTGGC TACTGTAAAG CAGTTGAAAG AATTTATTCA GCAACCAGAA 
AATAAGCTGG TACTAGTTAA ACAATTGGAT AATATCTTGG CTGCTGTACA TGACGTGCTT 
AATGAAAGTA GCAAATTGCT TCAGGAGTTG AGACAGGAGG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTGAGCTA TGAGGCTGAG AAGATCTTCA AGTGGATTTT TAGCAAATTT 
AGCTCATCTG CAAAAGATGA AGTTAAACTC CTCTACTTAT GTGCCACCTA CAAAGCACTA 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTGTAATGC AGCTTGTAAT GACCAGCCTG 
CAGTCTATTC TTGAAAATGT GGATACACCA GAATTGCTTT GTAAATGTGT TAAGTGCATT 
CTTTTGGTGG CTCGATGTTA CCCTCATATT TTCAGCACTA ATTTTAGGGA TACAGTTGAT 
ATATTAGTTG GATGGCATAT AGATCATACT CAGAAACCTT CGCTCACGCA GCAGGTATCT 
GGGTGGTTGC AGAGTTTGGA GCCATTTTGG GTAGCTGATC TTGCATTTTC TACTACTCTT 
CTTGGTCAGT TTCTGGAAGA CATGGAAGCA TATGCTGAGG ACCTCAGCCA TGTGGCCTCT 
GGGGAATCAG TGGATGAAGA TGTCCCTCCT CCATCAGTGT CATTACCAAA GCTGGCTGCA 
CTTCTCCGGG TATTTAGTAC TGTGGTGAGG AGCATTGGGG AACGCTTCAG CCCAATTCGG 
GGTCCTCCAA TTACTGAGGC ATATGTAACA GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACGGCTGCAA ACCAGGTGTT TTTTTCTGAG GCTGTGTTGA CAGCTGCTAA TGAGTGTGTT 
GGTGTTTTGC TCGGCAGCTT GGATCCTAGC ATGACTATAC ATTGTGACAT GGTCATTACA 
TATGGATTAG ACCAACTGGA GAATTGCCAG ACTTGTGGTA CCGATTATAT CATCTCAGTC 
TTGAATTTAC TCACGCTGAT TGTTGAACAG ATAAATACGA AACTGCCATC ATCATTTGTA 
GAAAAACTGT TTATACCATC ATCTAAACTA CTATTCTTGC GTTATCATAA AGAAAAAGAG 
GTTGTTGCTG TAGCCCATGC TGTTTATCAA GCAGTGCTCA GCTTGAAGAA TATTCCTGTT 
TTGGAGACTG CCTATAAGTT AATATTGGGA GAAATGACTT GTGCCCTAAA CAACCTCCTA 
CACAGTCTAC AACTTCCTGA GGCCTGTTCT GAAATAAAAC ATGAGGCTTT TAAGAATCAT 
GTGTTCAATG TAGACAATGC AAAATTTGTA GTTATATTTG ACCTCAGTGC CCTGACTACA 
ATTGGAAATG CCAAAAACTC ACTAATAGGG ATGTGGGCGC TATCTCCAAC TGTCTTTGCA 
CTTCTGAGTA AGAATCTGAT GATTGTGCAC AGTGACCTGG CTGTTCACTT CCCTGCCATT 
CAGTATGCTG TGCTCTACAC ATTGTATTCT CATTGTACCA GGCATGATCA CTTTATCTCT 
AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG TTTGATGGAG CTGTGATTAG CACTGTAACT 
ACGGCTACAA AGAAACATTT CTCAATTATA TTAAATCTTC TGGGAATATT ACTTAAGAAA 
GATAACCTTA ACCAGGACAC GAGGAAACTG TTAATGACTT GGGCTTTGGA AGCAGCTGTT 
TTAATGAAGA AGTCTGAAAC ATACGCACCT TTATTCTCTC TTCCGTCTTT CCATAAATTT 
TGCAAAGGCC TTTTAGCCAA CACTCTCGTT GAAGATGTGA ATATCTGTCT GCAGGCATGC 
AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG CCAGATGATC TTTTACAGAG ATGTGTCGAT 
GTTTGCCGTG TTCAACTAGT GCACAGTGGA ACTCGTATTC GACAAGCATT TGGAAAACTG 
TTGAAATCAA TTCCTTTAGA TGTTGTCCTA AGCAATAACA ATCACACAGA AATTCAAGAA 
ATTTCTTTAG CATTAAGAAG TCACATGAGT AAAGCACCAA GTAATACATT CCACCCCCAA 
GATTTCTCTG ATGTTATTAG TTTTATTTTG TATGGGAACT CTCATAGAAC AGGGAAGGAC 
AATTGGTTGG AAAGACTGTT CTATAGCTGC CAGAGACTGG ATAAGCGTGA CCAGTCAACA 
ATTCCACGCA ATCTCCTGAA GACAGATGCT GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT 
GCACAATTCA CTGTTCTTTC TAAGCTGAGA ACCCCACTGG GCAGAGCTCA AGACACCTTC 
CAGACAATTG AAGGTATCAT TCGAAGTCTC GCAGCTCACA CATTAAACCC TGATCAGGAT 
GTTAGTCAGT GGACAACTGC AGACAATGAT GAAGGCCATG GTAACAACCA ACTTAGACTT 
GTTCTTCTTC TGCAGTATCT GGAAAATCTG GAGAAATTAA TGTATAATGC ATACGAGGGA 
TGTGCTAATG CATTAACTTC ACCTCCCAAG GTCATTAGAA CTTTTTTCTA TACCAATCGC 
CAAACTTGTC AGGACTGGCT AACGCGGATT CGACTCTCCA TCATGAGGGT AGGATTGTTG 
GCAGGCCAGC CTGCAGTGAC AGTGAGACAT GGCTTTGACT TGCTTACAGA GATGAAAACA 
ACCAGCCTAT CTCAGGGGAA TGAATTGGAA GTAACCATTA TGATGGTGGT AGAAGCATTA 
TGTGAACTTC ATTGTCCTGA AGCTATACAG GGAATTGCTG TCTGGTCATC ATCTATTGTT 
GGAAAAAATC TTCTGTGGAT TAACTCAGTG GCTCAACAGG CTGAAGGGAG GTTTGAAAAG 
GCCTCTGTGG AGTACCAGGA ACACCTGTGT GCCATGACAG GTGTTGATTG CTGCATCTCC 
AGCTTTGACA AATCGGTGCT CACCTTAGCC AATGCTGGGC GTAACAGTGC CAGCCCGAAA 
CATTCTCTGA ATGGTGAATC CAGAAAAACT GTGCTGTCCA AACCGACTGA CTCTTCCCCT 
GAGGTTATAA ATTATTTAGG AAATAAAGCA TGTGAGTGCT ACATCTCAAT TGCCGATTGG 
GCTGCTGTGC AGGAATGGCA GAACGCTATC CATGACTTGA AAAAGAGTAC CAGTAGCACT 
TCCCTCAACC TGAAAGCTGA CTTCAACTAT ATAAAATCAT TAAGCAGCTT TGAGTCTGGA 
AAATTTGTTG AATGTACCGA GCAGTTAGAA TTGTTACCAG GAGAAAATAT CAATCTACTT 
GCTGGAGGAT CAAAAGAAAA AATAGACATG AAAAAACTGC TTCCTAACAT GTTAAGTCCG 
GATCCGAGGG AACTTCAGAA ATCCATTGAA GTTCAATTGT TAAGAAGTTC TGTTTGTTTG 
GCAACTGCTT TAAACCCGAT AGAACAAGAT CAGAAGTGGC AGTCTATAAC TGAAAATGTG 
GTAAAGTACT TGAAGCAAAC ATCCCGCATC GCTATTGGAC CTCTGAGACT TTCTACTTTA 
ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT ACCTTGCAGC TGTATTGCTC ATCTGCTTTG 
GAGAACACAG TTTCTAACAG ACTTTCAACA GAGGACTGTC TTATTCCACT CTTCAGTGAA 
GCTTTACGTT CATGTAAACA GCATGACGTG AGGCCATGGA TGCAGGCATT AAGGTATACT 
ATGTACCAGA ATCAGTTGTT GGAGAAAATT AAAGAACAAA CAGTCCCAAT TAGAAGCCAT 
CTCATGGAAT TAGGTCTAAC AGCAGCAAAA TTTGCTAGAA AACGAGGGAA TGTGTCCCTT 
GCAACAAGAC TGCTGGCACA GTGCAGTGAA GTTCAGCTGG GAAAGACCAC CACTGCACAG 
GATTTAGTCC AACATTTTAA AAAACTATCA ACCCAAGGTC AAGTGGATGA AAAATGGGGG 
CCCGAACTTG ATATTGAAAA AACCAAATTG CTTTATACAG CAGGCCAGTC AACACATGCA 
ATGGAAATGT TGAGTTCTTG TGCCATATCT TTCTGCAAGT CTGTGAAAGC TGAATATGCA 
GTTGCTAAAT CAATTCTGAC ACTGGCTAAA TGGATCCAGG CAGAATGGAA AGAGATTTCA 
GGACAGCTGA AACAGGTTTA CAGAGCTCAG CACCAACAGA ACTTCACAGG TCTTTCTACT 
TTGTCTAAAA ACATACTCAC TCTAATAGAA CTGCCATCTG TTAATACGAT GGAAGAAGAG 
TATCCTCGGA TCGAGAGTGA ATCTACAGTG CATATTGGAG TTGGAGAACC TGACTTCATT 
TTGGGACAGT TGTATCACCT GTCTTCAGTA CAGGCACCTG AAGTAGCCAA ATCTTGGGCA 
GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC AGAAAGGTGG TTGACAATGC CAGTCAGGGA 
GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA TCTGAAGTTC AGAATCTACT TCCAGACACT 
ATAACTGAGG AAGAGAAAGA GAGAATATAT GGTATTCTTG GACAGGCTGT GTGTCGGCCG 
GCGGGGATTC AGGATGAAGA TATAACACTT CAGATAACTG AGAGTGAAGA CAACGAAGAA 
GATGACATGG TTGATGTTAT CTGGCGTCAG TTGATATCAA GCTGCCCATG GCTTTCAGAA 
CTTGATGAAA GTGCAACTGA AGGAGTTATT AAAGTGTGGA GGAAAGTTGT AGATAGAATA 
TTCAGCCTGT ACAAACTCTC TTGCAGTGCA TACTTTACTT TCCTTAAACT CAACGCTGGT 
CAAATTCCTT TAGATGAGGA TGACCCTAGG CTGCATTTAA GTCACAGAGT GGAACAGAGC 
ACTGATGACA TGATTGTGAT GGCCACATTG CGCCTGCTGC GGTTGCTCGT GAAGCATGCT 
GGTGAGCTTC GGCAGTATCT GGAGCACGGC TTGGAGACAA CACCCACTGC ACCATGGAGA 
GGAATTATTC CGCAACTTTT CTCACGCTTA AACCACCCTG AAGTGTATGT GCGCCAAAGT 
ATTTGTAACC TTCTCTGCCG TGTGGCTCAA GATTCCCCAC ATCTCATATT GTATCCTGCA 
ATAGTGGGTA CCATATCGCT TAGTAGTGAA TCCCAGGCTT CAGGAAATAA ATTTTCCACT 
GCAATTCCAA CTTTACTTGG CAATATTCAA GGAGAAGAAT TGCTGGTTTC TGAATGTGAG 
GGAGGAAGTC CTCCTGCATC TCAGGATAGC AATAAGGATG AACCTAAAAG TGGATTAAAT 
GAAGACCAAG CCATGATGCA GGATTGTTAC AGCAAAATTG TAGATAAGCT GTCCTCTGCA 
AACCCCACCA TGGTATTACA GGTTCAGATG CTCGTGGCTG AACTGCGCAG GGTCACTGTG 
CTCTGGGATG AGCTCTGGCT GGGAGTTTTG CTGCAACAAC ACATGTATGT CCTGAGACGA 
ATTCAGCAGC TTGAAGATGA GGTGAAGAGA GTCCAGAACA ACAACACCTT ACGCAAAGAA 
GAGAAAATTG CAATCATGAG GGAGAAGCAC ACAGCTTTGA TGAAGCCCAT CGTATTTGCT 
TTGGAGCATG TGAGGAGTAT CACAGCGGCT CCTGCAGAAA CACCTCATGA AAAATGGTTT 
CAGGATAACT ATGGTGATGC CATTGAAAAT GCCCTAGAAA AACTGAAGAC TCCATTGAAC 
CCTGCAAAGC CTGGGAGCAG CTGGATTCCA TTTAAAGAGA TAATGCTAAG TTTGCAACAG 
AGAGCACAGA AACGTGCAAG TTACATCTTG CGTCTTGAAG AAATCAGTCC ATGGTTGGCT 
GCCATGACTA ACACTGAAAT TGCTCTTCCT GGGGAAGTCT CAGCCAGAGA CACTGTCACA 
ATCCATAGTG TGGGCGGAAC CATCACAATC TTACCGACTA AAACCAAGCC AAAGAAACTT 
CTCTTTCTTG GATCAGATGG GAAGAGCTAT CCTTATCTTT TCAAAGGACT GGAGGATTTA 
CATCTGGATG AGAGAATAAT GCAGTTCCTA TCTATTGTGA ATACCATGTT TGCTACAATT 
AATCGCCAAG AAACACCCCG GTTCCATGCT CGACACTATT CTGTAACACC ACTAGGAACA 
AGATCAGGAC TAATCCAGTG GGTAGATGGA GCCACACCCT TATTTGGTCT TTACAAACGA 
TGGCAACAAC GGGAAGCTGC CTTACAAGCA CAAAAGGCCC AAGATTCCTA CCAAACTCCT 
CAGAATCCTG GAATTGTACC CCGTCCTAGT GAACTTTATT ACAGTAAAAT TGGCCCTGCT 
TTGAAAACAG TTGGGCTTAG CCTGGATGTG TCCCGTCGGG ATTGGCCTCT TCATGTAATG 
AAGGCAGTAT TGGAAGAGTT AATGGAGGCC ACACCCCCGA ATCTCCTTGC CAAAGAGCTC 
TGGTCATCTT GCACAACACC TGATGAATGG TGGAGAGTTA CGCAGTCTTA TGCAAGATCT 
ACTGCAGTCA TGTCTATGGT TGGATACATA ATTGGCCTTG GAGACAGACA TCTGGATAAT 
GTTCTTATAG ATATGACGAC TGGAGAAGTT GTTCACATAG ATTACAATGT TTGCTTTGAA 
AAAGGTAAAA GCCTTAGAGT TCCTGAGAAA GTACCTTTTC GAATGACACA AAACATTGAA 
ACAGCACTGG GTGTAACTGG AGTAGAAGGT GTATTTAGGC TTTCATGTGA GCAGGTTTTA 
CACATTATGC GGCGTGGCAG AGAGACCCTG CTGACGCTGC TGGAGGCCTT TGTGTACGAC 
CCTCTGGTGG ACTGGACAGC AGGAGGCGAG GCTGGGTTTG CTGGTGCTGT CTATGGTGGA 
GGTGGCCAGC AGGCCGAGAG CAAGCAGAGC AAGAGAGAGA TGGAGCGAGA GATCACCCGC 
AGCCTGTTTT CTTCTAGAGT AGCTGAGATT AAGGTGAACT GGTTTAAGAA TAGAGATGAG 
ATGCTGGTTG TGCTTCCCAA GTTGGACGGT AGCTTAGATG AATACCTAAG CTTGCAAGAG 
CAACTGACAG ATGTGGAAAA ACTGCAGGGC AAACTACTGG AGGAAATAGA GTTTCTAGAA 
GGAGCTGAAG GGGTGGATCA TCCTTCTCAT ACTCTGCAAC ACAGGTATTC TGAGCACACC 
CAACTACAGA CTCAGCAAAG AGCTGTTCAG GAAGCAATCC AGGTGAAGCT GAATGAATTT 
GAACAATGGA TAACACATTA TCAGGCTGCA TTCAATAATT TAGAAGCAAC ACAGCTTGCA 
AGCTTGCTTC AAGAGATAAG CACACAAATG GACCTTGGTC CTCCAAGTTA CGTGCCAGCA 
ACAGCCTTTC TGCAGAATGC TGGTCAGGCC CACTTGATTA GCCAGTGCGA GCAGCTGGAG 
GGGGAGGTTG GTGCTCTCCT GCAGCAGAGG CGCTCCGTGC TCCGTGGCTG TCTGGAGCAA 
CTGCATCACT ATGCAACCGT GGCCCTGCAG TATCCGAAGG CCATATTTCA GAAACATCGA 
ATTGAACAGT GGAAGACCTG GATGGAAGAG CTCATCTGTA ACACCACAGT AGAGCGTTGT 
CAAGAGCTCT ATAGGAAATA TGAAATGCAA TATGCTCCCC AGCCACCCCC AACAGTGTGT 
CAGTTCATCA CTGCCACTGA AATGACCCTG CAGCGATACG CAGCAGACAT CAACAGCAGA 
CTTATTAGAC AAGTGGAACG CTTGAAACAG GAAGCTGTCA CTGTGCCAGT TTGTGAAGAT 
CAGTTGAAAG AAATTGAACG TTGCATTAAA GTTTTCCTTC ATGAGAATGG AGAAGAAGGA 
TCTTTGAGTC TAGCAAGTGT TATTATTTCT GCCCTTTGTA CCCTTACAAG GCGTAACCTG 
ATGATGGAAG GTGCAGCGTC AAGTGCTGGA GAACAGCTGG TTGATCTGAC TTCTCGGGAT 
GGAGCCTGGT TCTTGGAGGA ACTCTGCAGT ATGAGCGGAA ACGTCACCTG CTTGGTTCAG 
TTACTGAAGC AGTGCCACCT GGTGCCACAG GACTTAGATA TCCCGAACCC CATGGAAGCG 
TCTGAGACAG TTCACTTAGC CAATGGAGTG TATACCTCAC TTCAGGAATT GAATTCGAAT 
TTCCGGCAAA TCATATTTCC AGAAGCACTT CGATGTTTAA TGAAAGGGGA ATACACGTTA 
GAAAGTATGC TGCATGAACT GGACGGTCTT ATTGAGCAGA CCACCGATGG CGTTCCCCTG 
CAGACTCTAG TGGAATCTCT TCAGGCCTAC TTAAGAAACG CAGCTATGGG ACTGGAAGAA 
GAAACACATG CTCATTACAT CGATGTTGCC AGACTACTAC ATGCTCAGTA CGGTGAATTA 
ATCCAACCGA GAAATGGTTC AGTTGATGAA ACACCCAAAA TGTCAGCTGG CCAGATGCTT 
TTGGTAGCAT TCGATGGCAT GTTTGCTCAA GTTGAAACTG CTTTCAGCTT ATTAGTTGAA 
AAGTTGAACA AGATGGAAAT TCCCATAGCT TGGCGAAAGA TTGACATCAT AAGGGAAGCC 
AGGAGTACTC AAGTTAATTT TTTTGATGAT GATAATCACC GGCAGGTGCT AGAAGAGATT 
TTCTTTCTAA AAAGACTACA GACTATTAAG GAGTTCTTCA GGCTCTGTGG TACCTTTTCT 
AAAACATTGT CAGGATCAAG TTCACTTGAA GATCAGAATA CTGTGAATGG GCCTGTACAG 
ATTGTCAATG TGAAAACCCT TTTTAGAAAC TCTTGTTTCA GTGAAGACCA AATGGCCAAA 
CCTATCAAGG CATTCACAGC TGACTTTGTG AGGCAGCTCT TGATAGGGCT ACCCAACCAA 
GCCCTCGGAC TCACACTGTG CAGTTTTATC AGTGCTCTGG GTGTAGACAT CATTGCTCAA 
GTAGAGGCAA AGGACTTTGG TGCCGAAAGC AAAGTTTCTG TTGATGATCT CTGTAAGAAA 
GCGGTGGAAC ATAACATCCA GATAGGGAAG TTCTCTCAGC TGGTTATGAA CAGGGCAACT 
GTGTTAGCAA GTTCTTACGA CACTGCCTGG AAGAAGCATG ACTTGGTGCG AAGGCTAGAA 
ACCAGTATTT CTTCTTGTAA GACAAGCCTG CAGCGGGTTC AGCTGCATAT TGCCATGTTT 
CAGTGGCAAC ATGAAGATCT ACTTATCAAT AGACCACAAG CCATGTCAGT CACACCTCCC 
CCACGGTCTG CTATCCTAAC CAGCATGAAA AAGAAGCTGC ATACCCTGAG CCAGATTGAA 
ACTTCTATTG CAACAGTTCA GGAGAAGCTA GCTGCACTTG AATCAAGTAT TGAACAGCGA 
CTCAAGTGGG CAGGTGGTGC CAACCCTGCA TTGGCCCCTG TACTACAAGA TTTTGAAGCA 
ACGATAGCTG AAAGAAGAAA TCTTGTCCTT AAAGAGAGCC AAAGAGCAAG TCAGGTCACA 
TTTCTCTGCA GCAATATCAT TCATTTTGAA AGTTTACGAA CAAGAACTGC AGAAGCCTTA 
AACCTGGATG CGGCGTTATT TGAACTAATC AAGCGATGTC AGCAGATGTG TTCGTTTGCA 
TCACAGTTTA ACAGTTCAGT GTCTGAGTTA GAGCTTCGTT TATTACAGAG AGTGGACACT 
GGTCTTGAAC ATCCTATTGG CAGCTCTGAA TGGCTTTTGT CAGCACACAA ACAGTTGACC 
CAGGATATGT CTACTCAGAG GGCAATTCAG ACAGAGAAAG AGCAGCAGAT AGAAACGGTC 
TGTGAAACAA TTCAGAATCT GGTTGATAAT ATAAAGACTG TGCTCACTGG TCATAACCGA 
CAGCTTGGAG ATGTCAAACA TCTCTTGAAA GCTATGGCTA AGGATGAAGA AGCTGCTCTG 
GCAGATGGTG AAGATGTTCC CTATGAGAAC AGTGTTAGGC AGTTTTTGGG TGAATATAAA 
TCATGGCAAG ACAACATTCA AACAGTTCTA TTTACATTAG TCCAGGCTAT GGGTCAGGTT 
CGAAGTCAAG AACACGTTGA AATGCTCCAG GAAATCACTC CCACCTTGAA AGAACTGAAA 
ACACAAAGTC AGAGTATCTA TAATAATTTA GTGAGTTTTG CATCACCCTT AGTCACCGAT 
GCAACAAATG AATGTTCGAG TCCAACGTCA TCTGCTACTT ATCAGCCATC CTTCGCTGCA 
GCAGTCCGGA GTAACACTGG CCAGAAGACT CAGCCTGATG TCATGTCACA GAATGCTAGA 
AAGCTGATCC AAAAAAATCT TGCTACATCA GCTGATACTC CACCAAGCAC CGTTCCAGGA 
ACTGGCAAGA GTGTTGCTTG TAGTCCTAAA AAGGCAGTCA GAGACCCTAA AACTGGGAAA 
GCGGTGCAAG AGAGAAACTC CTATGCAGTG AGTGTGTGGA AGAGAGTGAA AGCCAAGTTA 
GAGGGCCGAG ATGTTGATCC GAATAGGAGG ATGTCAGTTG CTGAACAGGT TGACTATGTC 
ATTAAGGAAG CAACTAATCT AGATAACTTG GCTCAGCTGT ATGAAGGTTG GACAGCCTGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055907.3
CDS	368..11353
Translation	MSRRAPGSRLSSGGGGGGTKYPRSWNDWQPRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSVQHGSRALATKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	NM_015092.5
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015092.5

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGGGTT AACAATGTGA CCAGCCCTGA GTTCACCAGT 
GTTCAGCATG GCAGTCGTGC TTTAGCCACC AAAGACATGA GGAAATCACA GGAGAGATCG 
ATGTCTTATT CTGATGAGTC TCGACTGTCG AATCTTCTTC GGAGGATCAC CCGGGAAGAC 
GACAGAGACC GAAGATTGGC TACTGTAAAG CAGTTGAAAG AATTTATTCA GCAACCAGAA 
AATAAGCTGG TACTAGTTAA ACAATTGGAT AATATCTTGG CTGCTGTACA TGACGTGCTT 
AATGAAAGTA GCAAATTGCT TCAGGAGTTG AGACAGGAGG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTGAGCTA TGAGGCTGAG AAGATCTTCA AGTGGATTTT TAGCAAATTT 
AGCTCATCTG CAAAAGATGA AGTTAAACTC CTCTACTTAT GTGCCACCTA CAAAGCACTA 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTGTAATGC AGCTTGTAAT GACCAGCCTG 
CAGTCTATTC TTGAAAATGT GGATACACCA GAATTGCTTT GTAAATGTGT TAAGTGCATT 
CTTTTGGTGG CTCGATGTTA CCCTCATATT TTCAGCACTA ATTTTAGGGA TACAGTTGAT 
ATATTAGTTG GATGGCATAT AGATCATACT CAGAAACCTT CGCTCACGCA GCAGGTATCT 
GGGTGGTTGC AGAGTTTGGA GCCATTTTGG GTAGCTGATC TTGCATTTTC TACTACTCTT 
CTTGGTCAGT TTCTGGAAGA CATGGAAGCA TATGCTGAGG ACCTCAGCCA TGTGGCCTCT 
GGGGAATCAG TGGATGAAGA TGTCCCTCCT CCATCAGTGT CATTACCAAA GCTGGCTGCA 
CTTCTCCGGG TATTTAGTAC TGTGGTGAGG AGCATTGGGG AACGCTTCAG CCCAATTCGG 
GGTCCTCCAA TTACTGAGGC ATATGTAACA GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACGGCTGCAA ACCAGGTGTT TTTTTCTGAG GCTGTGTTGA CAGCTGCTAA TGAGTGTGTT 
GGTGTTTTGC TCGGCAGCTT GGATCCTAGC ATGACTATAC ATTGTGACAT GGTCATTACA 
TATGGATTAG ACCAACTGGA GAATTGCCAG ACTTGTGGTA CCGATTATAT CATCTCAGTC 
TTGAATTTAC TCACGCTGAT TGTTGAACAG ATAAATACGA AACTGCCATC ATCATTTGTA 
GAAAAACTGT TTATACCATC ATCTAAACTA CTATTCTTGC GTTATCATAA AGAAAAAGAG 
GTTGTTGCTG TAGCCCATGC TGTTTATCAA GCAGTGCTCA GCTTGAAGAA TATTCCTGTT 
TTGGAGACTG CCTATAAGTT AATATTGGGA GAAATGACTT GTGCCCTAAA CAACCTCCTA 
CACAGTCTAC AACTTCCTGA GGCCTGTTCT GAAATAAAAC ATGAGGCTTT TAAGAATCAT 
GTGTTCAATG TAGACAATGC AAAATTTGTA GTTATATTTG ACCTCAGTGC CCTGACTACA 
ATTGGAAATG CCAAAAACTC ACTAATAGGG ATGTGGGCGC TATCTCCAAC TGTCTTTGCA 
CTTCTGAGTA AGAATCTGAT GATTGTGCAC AGTGACCTGG CTGTTCACTT CCCTGCCATT 
CAGTATGCTG TGCTCTACAC ATTGTATTCT CATTGTACCA GGCATGATCA CTTTATCTCT 
AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG TTTGATGGAG CTGTGATTAG CACTGTAACT 
ACGGCTACAA AGAAACATTT CTCAATTATA TTAAATCTTC TGGGAATATT ACTTAAGAAA 
GATAACCTTA ACCAGGACAC GAGGAAACTG TTAATGACTT GGGCTTTGGA AGCAGCTGTT 
TTAATGAAGA AGTCTGAAAC ATACGCACCT TTATTCTCTC TTCCGTCTTT CCATAAATTT 
TGCAAAGGCC TTTTAGCCAA CACTCTCGTT GAAGATGTGA ATATCTGTCT GCAGGCATGC 
AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG CCAGATGATC TTTTACAGAG ATGTGTCGAT 
GTTTGCCGTG TTCAACTAGT GCACAGTGGA ACTCGTATTC GACAAGCATT TGGAAAACTG 
TTGAAATCAA TTCCTTTAGA TGTTGTCCTA AGCAATAACA ATCACACAGA AATTCAAGAA 
ATTTCTTTAG CATTAAGAAG TCACATGAGT AAAGCACCAA GTAATACATT CCACCCCCAA 
GATTTCTCTG ATGTTATTAG TTTTATTTTG TATGGGAACT CTCATAGAAC AGGGAAGGAC 
AATTGGTTGG AAAGACTGTT CTATAGCTGC CAGAGACTGG ATAAGCGTGA CCAGTCAACA 
ATTCCACGCA ATCTCCTGAA GACAGATGCT GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT 
GCACAATTCA CTGTTCTTTC TAAGCTGAGA ACCCCACTGG GCAGAGCTCA AGACACCTTC 
CAGACAATTG AAGGTATCAT TCGAAGTCTC GCAGCTCACA CATTAAACCC TGATCAGGAT 
GTTAGTCAGT GGACAACTGC AGACAATGAT GAAGGCCATG GTAACAACCA ACTTAGACTT 
GTTCTTCTTC TGCAGTATCT GGAAAATCTG GAGAAATTAA TGTATAATGC ATACGAGGGA 
TGTGCTAATG CATTAACTTC ACCTCCCAAG GTCATTAGAA CTTTTTTCTA TACCAATCGC 
CAAACTTGTC AGGACTGGCT AACGCGGATT CGACTCTCCA TCATGAGGGT AGGATTGTTG 
GCAGGCCAGC CTGCAGTGAC AGTGAGACAT GGCTTTGACT TGCTTACAGA GATGAAAACA 
ACCAGCCTAT CTCAGGGGAA TGAATTGGAA GTAACCATTA TGATGGTGGT AGAAGCATTA 
TGTGAACTTC ATTGTCCTGA AGCTATACAG GGAATTGCTG TCTGGTCATC ATCTATTGTT 
GGAAAAAATC TTCTGTGGAT TAACTCAGTG GCTCAACAGG CTGAAGGGAG GTTTGAAAAG 
GCCTCTGTGG AGTACCAGGA ACACCTGTGT GCCATGACAG GTGTTGATTG CTGCATCTCC 
AGCTTTGACA AATCGGTGCT CACCTTAGCC AATGCTGGGC GTAACAGTGC CAGCCCGAAA 
CATTCTCTGA ATGGTGAATC CAGAAAAACT GTGCTGTCCA AACCGACTGA CTCTTCCCCT 
GAGGTTATAA ATTATTTAGG AAATAAAGCA TGTGAGTGCT ACATCTCAAT TGCCGATTGG 
GCTGCTGTGC AGGAATGGCA GAACGCTATC CATGACTTGA AAAAGAGTAC CAGTAGCACT 
TCCCTCAACC TGAAAGCTGA CTTCAACTAT ATAAAATCAT TAAGCAGCTT TGAGTCTGGA 
AAATTTGTTG AATGTACCGA GCAGTTAGAA TTGTTACCAG GAGAAAATAT CAATCTACTT 
GCTGGAGGAT CAAAAGAAAA AATAGACATG AAAAAACTGC TTCCTAACAT GTTAAGTCCG 
GATCCGAGGG AACTTCAGAA ATCCATTGAA GTTCAATTGT TAAGAAGTTC TGTTTGTTTG 
GCAACTGCTT TAAACCCGAT AGAACAAGAT CAGAAGTGGC AGTCTATAAC TGAAAATGTG 
GTAAAGTACT TGAAGCAAAC ATCCCGCATC GCTATTGGAC CTCTGAGACT TTCTACTTTA 
ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT ACCTTGCAGC TGTATTGCTC ATCTGCTTTG 
GAGAACACAG TTTCTAACAG ACTTTCAACA GAGGACTGTC TTATTCCACT CTTCAGTGAA 
GCTTTACGTT CATGTAAACA GCATGACGTG AGGCCATGGA TGCAGGCATT AAGGTATACT 
ATGTACCAGA ATCAGTTGTT GGAGAAAATT AAAGAACAAA CAGTCCCAAT TAGAAGCCAT 
CTCATGGAAT TAGGTCTAAC AGCAGCAAAA TTTGCTAGAA AACGAGGGAA TGTGTCCCTT 
GCAACAAGAC TGCTGGCACA GTGCAGTGAA GTTCAGCTGG GAAAGACCAC CACTGCACAG 
GATTTAGTCC AACATTTTAA AAAACTATCA ACCCAAGGTC AAGTGGATGA AAAATGGGGG 
CCCGAACTTG ATATTGAAAA AACCAAATTG CTTTATACAG CAGGCCAGTC AACACATGCA 
ATGGAAATGT TGAGTTCTTG TGCCATATCT TTCTGCAAGT CTGTGAAAGC TGAATATGCA 
GTTGCTAAAT CAATTCTGAC ACTGGCTAAA TGGATCCAGG CAGAATGGAA AGAGATTTCA 
GGACAGCTGA AACAGGTTTA CAGAGCTCAG CACCAACAGA ACTTCACAGG TCTTTCTACT 
TTGTCTAAAA ACATACTCAC TCTAATAGAA CTGCCATCTG TTAATACGAT GGAAGAAGAG 
TATCCTCGGA TCGAGAGTGA ATCTACAGTG CATATTGGAG TTGGAGAACC TGACTTCATT 
TTGGGACAGT TGTATCACCT GTCTTCAGTA CAGGCACCTG AAGTAGCCAA ATCTTGGGCA 
GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC AGAAAGGTGG TTGACAATGC CAGTCAGGGA 
GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA TCTGAAGTTC AGAATCTACT TCCAGACACT 
ATAACTGAGG AAGAGAAAGA GAGAATATAT GGTATTCTTG GACAGGCTGT GTGTCGGCCG 
GCGGGGATTC AGGATGAAGA TATAACACTT CAGATAACTG AGAGTGAAGA CAACGAAGAA 
GATGACATGG TTGATGTTAT CTGGCGTCAG TTGATATCAA GCTGCCCATG GCTTTCAGAA 
CTTGATGAAA GTGCAACTGA AGGAGTTATT AAAGTGTGGA GGAAAGTTGT AGATAGAATA 
TTCAGCCTGT ACAAACTCTC TTGCAGTGCA TACTTTACTT TCCTTAAACT CAACGCTGGT 
CAAATTCCTT TAGATGAGGA TGACCCTAGG CTGCATTTAA GTCACAGAGT GGAACAGAGC 
ACTGATGACA TGATTGTGAT GGCCACATTG CGCCTGCTGC GGTTGCTCGT GAAGCATGCT 
GGTGAGCTTC GGCAGTATCT GGAGCACGGC TTGGAGACAA CACCCACTGC ACCATGGAGA 
GGAATTATTC CGCAACTTTT CTCACGCTTA AACCACCCTG AAGTGTATGT GCGCCAAAGT 
ATTTGTAACC TTCTCTGCCG TGTGGCTCAA GATTCCCCAC ATCTCATATT GTATCCTGCA 
ATAGTGGGTA CCATATCGCT TAGTAGTGAA TCCCAGGCTT CAGGAAATAA ATTTTCCACT 
GCAATTCCAA CTTTACTTGG CAATATTCAA GGAGAAGAAT TGCTGGTTTC TGAATGTGAG 
GGAGGAAGTC CTCCTGCATC TCAGGATAGC AATAAGGATG AACCTAAAAG TGGATTAAAT 
GAAGACCAAG CCATGATGCA GGATTGTTAC AGCAAAATTG TAGATAAGCT GTCCTCTGCA 
AACCCCACCA TGGTATTACA GGTTCAGATG CTCGTGGCTG AACTGCGCAG GGTCACTGTG 
CTCTGGGATG AGCTCTGGCT GGGAGTTTTG CTGCAACAAC ACATGTATGT CCTGAGACGA 
ATTCAGCAGC TTGAAGATGA GGTGAAGAGA GTCCAGAACA ACAACACCTT ACGCAAAGAA 
GAGAAAATTG CAATCATGAG GGAGAAGCAC ACAGCTTTGA TGAAGCCCAT CGTATTTGCT 
TTGGAGCATG TGAGGAGTAT CACAGCGGCT CCTGCAGAAA CACCTCATGA AAAATGGTTT 
CAGGATAACT ATGGTGATGC CATTGAAAAT GCCCTAGAAA AACTGAAGAC TCCATTGAAC 
CCTGCAAAGC CTGGGAGCAG CTGGATTCCA TTTAAAGAGA TAATGCTAAG TTTGCAACAG 
AGAGCACAGA AACGTGCAAG TTACATCTTG CGTCTTGAAG AAATCAGTCC ATGGTTGGCT 
GCCATGACTA ACACTGAAAT TGCTCTTCCT GGGGAAGTCT CAGCCAGAGA CACTGTCACA 
ATCCATAGTG TGGGCGGAAC CATCACAATC TTACCGACTA AAACCAAGCC AAAGAAACTT 
CTCTTTCTTG GATCAGATGG GAAGAGCTAT CCTTATCTTT TCAAAGGACT GGAGGATTTA 
CATCTGGATG AGAGAATAAT GCAGTTCCTA TCTATTGTGA ATACCATGTT TGCTACAATT 
AATCGCCAAG AAACACCCCG GTTCCATGCT CGACACTATT CTGTAACACC ACTAGGAACA 
AGATCAGGAC TAATCCAGTG GGTAGATGGA GCCACACCCT TATTTGGTCT TTACAAACGA 
TGGCAACAAC GGGAAGCTGC CTTACAAGCA CAAAAGGCCC AAGATTCCTA CCAAACTCCT 
CAGAATCCTG GAATTGTACC CCGTCCTAGT GAACTTTATT ACAGTAAAAT TGGCCCTGCT 
TTGAAAACAG TTGGGCTTAG CCTGGATGTG TCCCGTCGGG ATTGGCCTCT TCATGTAATG 
AAGGCAGTAT TGGAAGAGTT AATGGAGGCC ACACCCCCGA ATCTCCTTGC CAAAGAGCTC 
TGGTCATCTT GCACAACACC TGATGAATGG TGGAGAGTTA CGCAGTCTTA TGCAAGATCT 
ACTGCAGTCA TGTCTATGGT TGGATACATA ATTGGCCTTG GAGACAGACA TCTGGATAAT 
GTTCTTATAG ATATGACGAC TGGAGAAGTT GTTCACATAG ATTACAATGT TTGCTTTGAA 
AAAGGTAAAA GCCTTAGAGT TCCTGAGAAA GTACCTTTTC GAATGACACA AAACATTGAA 
ACAGCACTGG GTGTAACTGG AGTAGAAGGT GTATTTAGGC TTTCATGTGA GCAGGTTTTA 
CACATTATGC GGCGTGGCAG AGAGACCCTG CTGACGCTGC TGGAGGCCTT TGTGTACGAC 
CCTCTGGTGG ACTGGACAGC AGGAGGCGAG GCTGGGTTTG CTGGTGCTGT CTATGGTGGA 
GGTGGCCAGC AGGCCGAGAG CAAGCAGAGC AAGAGAGAGA TGGAGCGAGA GATCACCCGC 
AGCCTGTTTT CTTCTAGAGT AGCTGAGATT AAGGTGAACT GGTTTAAGAA TAGAGATGAG 
ATGCTGGTTG TGCTTCCCAA GTTGGACGGT AGCTTAGATG AATACCTAAG CTTGCAAGAG 
CAACTGACAG ATGTGGAAAA ACTGCAGGGC AAACTACTGG AGGAAATAGA GTTTCTAGAA 
GGAGCTGAAG GGGTGGATCA TCCTTCTCAT ACTCTGCAAC ACAGGTATTC TGAGCACACC 
CAACTACAGA CTCAGCAAAG AGCTGTTCAG GAAGCAATCC AGGTGAAGCT GAATGAATTT 
GAACAATGGA TAACACATTA TCAGGCTGCA TTCAATAATT TAGAAGCAAC ACAGCTTGCA 
AGCTTGCTTC AAGAGATAAG CACACAAATG GACCTTGGTC CTCCAAGTTA CGTGCCAGCA 
ACAGCCTTTC TGCAGAATGC TGGTCAGGCC CACTTGATTA GCCAGTGCGA GCAGCTGGAG 
GGGGAGGTTG GTGCTCTCCT GCAGCAGAGG CGCTCCGTGC TCCGTGGCTG TCTGGAGCAA 
CTGCATCACT ATGCAACCGT GGCCCTGCAG TATCCGAAGG CCATATTTCA GAAACATCGA 
ATTGAACAGT GGAAGACCTG GATGGAAGAG CTCATCTGTA ACACCACAGT AGAGCGTTGT 
CAAGAGCTCT ATAGGAAATA TGAAATGCAA TATGCTCCCC AGCCACCCCC AACAGTGTGT 
CAGTTCATCA CTGCCACTGA AATGACCCTG CAGCGATACG CAGCAGACAT CAACAGCAGA 
CTTATTAGAC AAGTGGAACG CTTGAAACAG GAAGCTGTCA CTGTGCCAGT TTGTGAAGAT 
CAGTTGAAAG AAATTGAACG TTGCATTAAA GTTTTCCTTC ATGAGAATGG AGAAGAAGGA 
TCTTTGAGTC TAGCAAGTGT TATTATTTCT GCCCTTTGTA CCCTTACAAG GCGTAACCTG 
ATGATGGAAG GTGCAGCGTC AAGTGCTGGA GAACAGCTGG TTGATCTGAC TTCTCGGGAT 
GGAGCCTGGT TCTTGGAGGA ACTCTGCAGT ATGAGCGGAA ACGTCACCTG CTTGGTTCAG 
TTACTGAAGC AGTGCCACCT GGTGCCACAG GACTTAGATA TCCCGAACCC CATGGAAGCG 
TCTGAGACAG TTCACTTAGC CAATGGAGTG TATACCTCAC TTCAGGAATT GAATTCGAAT 
TTCCGGCAAA TCATATTTCC AGAAGCACTT CGATGTTTAA TGAAAGGGGA ATACACGTTA 
GAAAGTATGC TGCATGAACT GGACGGTCTT ATTGAGCAGA CCACCGATGG CGTTCCCCTG 
CAGACTCTAG TGGAATCTCT TCAGGCCTAC TTAAGAAACG CAGCTATGGG ACTGGAAGAA 
GAAACACATG CTCATTACAT CGATGTTGCC AGACTACTAC ATGCTCAGTA CGGTGAATTA 
ATCCAACCGA GAAATGGTTC AGTTGATGAA ACACCCAAAA TGTCAGCTGG CCAGATGCTT 
TTGGTAGCAT TCGATGGCAT GTTTGCTCAA GTTGAAACTG CTTTCAGCTT ATTAGTTGAA 
AAGTTGAACA AGATGGAAAT TCCCATAGCT TGGCGAAAGA TTGACATCAT AAGGGAAGCC 
AGGAGTACTC AAGTTAATTT TTTTGATGAT GATAATCACC GGCAGGTGCT AGAAGAGATT 
TTCTTTCTAA AAAGACTACA GACTATTAAG GAGTTCTTCA GGCTCTGTGG TACCTTTTCT 
AAAACATTGT CAGGATCAAG TTCACTTGAA GATCAGAATA CTGTGAATGG GCCTGTACAG 
ATTGTCAATG TGAAAACCCT TTTTAGAAAC TCTTGTTTCA GTGAAGACCA AATGGCCAAA 
CCTATCAAGG CATTCACAGC TGACTTTGTG AGGCAGCTCT TGATAGGGCT ACCCAACCAA 
GCCCTCGGAC TCACACTGTG CAGTTTTATC AGTGCTCTGG GTGTAGACAT CATTGCTCAA 
GTAGAGGCAA AGGACTTTGG TGCCGAAAGC AAAGTTTCTG TTGATGATCT CTGTAAGAAA 
GCGGTGGAAC ATAACATCCA GATAGGGAAG TTCTCTCAGC TGGTTATGAA CAGGGCAACT 
GTGTTAGCAA GTTCTTACGA CACTGCCTGG AAGAAGCATG ACTTGGTGCG AAGGCTAGAA 
ACCAGTATTT CTTCTTGTAA GACAAGCCTG CAGCGGGTTC AGCTGCATAT TGCCATGTTT 
CAGTGGCAAC ATGAAGATCT ACTTATCAAT AGACCACAAG CCATGTCAGT CACACCTCCC 
CCACGGTCTG CTATCCTAAC CAGCATGAAA AAGAAGCTGC ATACCCTGAG CCAGATTGAA 
ACTTCTATTG CAACAGTTCA GGAGAAGCTA GCTGCACTTG AATCAAGTAT TGAACAGCGA 
CTCAAGTGGG CAGGTGGTGC CAACCCTGCA TTGGCCCCTG TACTACAAGA TTTTGAAGCA 
ACGATAGCTG AAAGAAGAAA TCTTGTCCTT AAAGAGAGCC AAAGAGCAAG TCAGGTCACA 
TTTCTCTGCA GCAATATCAT TCATTTTGAA AGTTTACGAA CAAGAACTGC AGAAGCCTTA 
AACCTGGATG CGGCGTTATT TGAACTAATC AAGCGATGTC AGCAGATGTG TTCGTTTGCA 
TCACAGTTTA ACAGTTCAGT GTCTGAGTTA GAGCTTCGTT TATTACAGAG AGTGGACACT 
GGTCTTGAAC ATCCTATTGG CAGCTCTGAA TGGCTTTTGT CAGCACACAA ACAGTTGACC 
CAGGATATGT CTACTCAGAG GGCAATTCAG ACAGAGAAAG AGCAGCAGAT AGAAACGGTC 
TGTGAAACAA TTCAGAATCT GGTTGATAAT ATAAAGACTG TGCTCACTGG TCATAACCGA 
CAGCTTGGAG ATGTCAAACA TCTCTTGAAA GCTATGGCTA AGGATGAAGA AGCTGCTCTG 
GCAGATGGTG AAGATGTTCC CTATGAGAAC AGTGTTAGGC AGTTTTTGGG TGAATATAAA 
TCATGGCAAG ACAACATTCA AACAGTTCTA TTTACATTAG TCCAGGCTAT GGGTCAGGTT 
CGAAGTCAAG AACACGTTGA AATGCTCCAG GAAATCACTC CCACCTTGAA AGAACTGAAA 
ACACAAAGTC AGAGTATCTA TAATAATTTA GTGAGTTTTG CATCACCCTT AGTCACCGAT 
GCAACAAATG AATGTTCGAG TCCAACGTCA TCTGCTACTT ATCAGCCATC CTTCGCTGCA 
GCAGTCCGGA GTAACACTGG CCAGAAGACT CAGCCTGATG TCATGTCACA GAATGCTAGA 
AAGCTGATCC AAAAAAATCT TGCTACATCA GCTGATACTC CACCAAGCAC CGTTCCAGGA 
ACTGGCAAGA GTGTTGCTTG TAGTCCTAAA AAGGCAGTCA GAGACCCTAA AACTGGGAAA 
GCGGTGCAAG AGAGAAACTC CTATGCAGTG AGTGTGTGGA AGAGAGTGAA AGCCAAGTTA 
GAGGGCCGAG ATGTTGATCC GAATAGGAGG ATGTCAGTTG CTGAACAGGT TGACTATGTC 
ATTAAGGAAG CAACTAATCT AGATAACTTG GCTCAGCTGT ATGAAGGTTG GACAGCCTGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( NM_015092.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055907.3 Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), mRNA.

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CloneID	OHu26275
Clone ID Related Accession (Same CDS sequence)	NM_015092.4
Accession Version	NM_015092.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA433527.1, AW503964.1, DA854035.1, AB061371.1, BE079340.1, AY014957.1, AL833298.1, BC037335.1, AK025794.1 and AW088221.1. On Jan 1, 2009 this sequence version replaced NM_015092.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB061371.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGGGTT AACAATGTGA CCAGCCCTGA GTTCACCAGT 
GTTCAGCATG GCAGTCGTGC TTTAGCCACC AAAGACATGA GGAAATCACA GGAGAGATCG 
ATGTCTTATT CTGATGAGTC TCGACTGTCG AATCTTCTTC GGAGGATCAC CCGGGAAGAC 
GACAGAGACC GAAGATTGGC TACTGTAAAG CAGTTGAAAG AATTTATTCA GCAACCAGAA 
AATAAGCTGG TACTAGTTAA ACAATTGGAT AATATCTTGG CTGCTGTACA TGACGTGCTT 
AATGAAAGTA GCAAATTGCT TCAGGAGTTG AGACAGGAGG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTGAGCTA TGAGGCTGAG AAGATCTTCA AGTGGATTTT TAGCAAATTT 
AGCTCATCTG CAAAAGATGA AGTTAAACTC CTCTACTTAT GTGCCACCTA CAAAGCACTA 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTGTAATGC AGCTTGTAAT GACCAGCCTG 
CAGTCTATTC TTGAAAATGT GGATACACCA GAATTGCTTT GTAAATGTGT TAAGTGCATT 
CTTTTGGTGG CTCGATGTTA CCCTCATATT TTCAGCACTA ATTTTAGGGA TACAGTTGAT 
ATATTAGTTG GATGGCATAT AGATCATACT CAGAAACCTT CGCTCACGCA GCAGGTATCT 
GGGTGGTTGC AGAGTTTGGA GCCATTTTGG GTAGCTGATC TTGCATTTTC TACTACTCTT 
CTTGGTCAGT TTCTGGAAGA CATGGAAGCA TATGCTGAGG ACCTCAGCCA TGTGGCCTCT 
GGGGAATCAG TGGATGAAGA TGTCCCTCCT CCATCAGTGT CATTACCAAA GCTGGCTGCA 
CTTCTCCGGG TATTTAGTAC TGTGGTGAGG AGCATTGGGG AACGCTTCAG CCCAATTCGG 
GGTCCTCCAA TTACTGAGGC ATATGTAACA GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACGGCTGCAA ACCAGGTGTT TTTTTCTGAG GCTGTGTTGA CAGCTGCTAA TGAGTGTGTT 
GGTGTTTTGC TCGGCAGCTT GGATCCTAGC ATGACTATAC ATTGTGACAT GGTCATTACA 
TATGGATTAG ACCAACTGGA GAATTGCCAG ACTTGTGGTA CCGATTATAT CATCTCAGTC 
TTGAATTTAC TCACGCTGAT TGTTGAACAG ATAAATACGA AACTGCCATC ATCATTTGTA 
GAAAAACTGT TTATACCATC ATCTAAACTA CTATTCTTGC GTTATCATAA AGAAAAAGAG 
GTTGTTGCTG TAGCCCATGC TGTTTATCAA GCAGTGCTCA GCTTGAAGAA TATTCCTGTT 
TTGGAGACTG CCTATAAGTT AATATTGGGA GAAATGACTT GTGCCCTAAA CAACCTCCTA 
CACAGTCTAC AACTTCCTGA GGCCTGTTCT GAAATAAAAC ATGAGGCTTT TAAGAATCAT 
GTGTTCAATG TAGACAATGC AAAATTTGTA GTTATATTTG ACCTCAGTGC CCTGACTACA 
ATTGGAAATG CCAAAAACTC ACTAATAGGG ATGTGGGCGC TATCTCCAAC TGTCTTTGCA 
CTTCTGAGTA AGAATCTGAT GATTGTGCAC AGTGACCTGG CTGTTCACTT CCCTGCCATT 
CAGTATGCTG TGCTCTACAC ATTGTATTCT CATTGTACCA GGCATGATCA CTTTATCTCT 
AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG TTTGATGGAG CTGTGATTAG CACTGTAACT 
ACGGCTACAA AGAAACATTT CTCAATTATA TTAAATCTTC TGGGAATATT ACTTAAGAAA 
GATAACCTTA ACCAGGACAC GAGGAAACTG TTAATGACTT GGGCTTTGGA AGCAGCTGTT 
TTAATGAAGA AGTCTGAAAC ATACGCACCT TTATTCTCTC TTCCGTCTTT CCATAAATTT 
TGCAAAGGCC TTTTAGCCAA CACTCTCGTT GAAGATGTGA ATATCTGTCT GCAGGCATGC 
AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG CCAGATGATC TTTTACAGAG ATGTGTCGAT 
GTTTGCCGTG TTCAACTAGT GCACAGTGGA ACTCGTATTC GACAAGCATT TGGAAAACTG 
TTGAAATCAA TTCCTTTAGA TGTTGTCCTA AGCAATAACA ATCACACAGA AATTCAAGAA 
ATTTCTTTAG CATTAAGAAG TCACATGAGT AAAGCACCAA GTAATACATT CCACCCCCAA 
GATTTCTCTG ATGTTATTAG TTTTATTTTG TATGGGAACT CTCATAGAAC AGGGAAGGAC 
AATTGGTTGG AAAGACTGTT CTATAGCTGC CAGAGACTGG ATAAGCGTGA CCAGTCAACA 
ATTCCACGCA ATCTCCTGAA GACAGATGCT GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT 
GCACAATTCA CTGTTCTTTC TAAGCTGAGA ACCCCACTGG GCAGAGCTCA AGACACCTTC 
CAGACAATTG AAGGTATCAT TCGAAGTCTC GCAGCTCACA CATTAAACCC TGATCAGGAT 
GTTAGTCAGT GGACAACTGC AGACAATGAT GAAGGCCATG GTAACAACCA ACTTAGACTT 
GTTCTTCTTC TGCAGTATCT GGAAAATCTG GAGAAATTAA TGTATAATGC ATACGAGGGA 
TGTGCTAATG CATTAACTTC ACCTCCCAAG GTCATTAGAA CTTTTTTCTA TACCAATCGC 
CAAACTTGTC AGGACTGGCT AACGCGGATT CGACTCTCCA TCATGAGGGT AGGATTGTTG 
GCAGGCCAGC CTGCAGTGAC AGTGAGACAT GGCTTTGACT TGCTTACAGA GATGAAAACA 
ACCAGCCTAT CTCAGGGGAA TGAATTGGAA GTAACCATTA TGATGGTGGT AGAAGCATTA 
TGTGAACTTC ATTGTCCTGA AGCTATACAG GGAATTGCTG TCTGGTCATC ATCTATTGTT 
GGAAAAAATC TTCTGTGGAT TAACTCAGTG GCTCAACAGG CTGAAGGGAG GTTTGAAAAG 
GCCTCTGTGG AGTACCAGGA ACACCTGTGT GCCATGACAG GTGTTGATTG CTGCATCTCC 
AGCTTTGACA AATCGGTGCT CACCTTAGCC AATGCTGGGC GTAACAGTGC CAGCCCGAAA 
CATTCTCTGA ATGGTGAATC CAGAAAAACT GTGCTGTCCA AACCGACTGA CTCTTCCCCT 
GAGGTTATAA ATTATTTAGG AAATAAAGCA TGTGAGTGCT ACATCTCAAT TGCCGATTGG 
GCTGCTGTGC AGGAATGGCA GAACGCTATC CATGACTTGA AAAAGAGTAC CAGTAGCACT 
TCCCTCAACC TGAAAGCTGA CTTCAACTAT ATAAAATCAT TAAGCAGCTT TGAGTCTGGA 
AAATTTGTTG AATGTACCGA GCAGTTAGAA TTGTTACCAG GAGAAAATAT CAATCTACTT 
GCTGGAGGAT CAAAAGAAAA AATAGACATG AAAAAACTGC TTCCTAACAT GTTAAGTCCG 
GATCCGAGGG AACTTCAGAA ATCCATTGAA GTTCAATTGT TAAGAAGTTC TGTTTGTTTG 
GCAACTGCTT TAAACCCGAT AGAACAAGAT CAGAAGTGGC AGTCTATAAC TGAAAATGTG 
GTAAAGTACT TGAAGCAAAC ATCCCGCATC GCTATTGGAC CTCTGAGACT TTCTACTTTA 
ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT ACCTTGCAGC TGTATTGCTC ATCTGCTTTG 
GAGAACACAG TTTCTAACAG ACTTTCAACA GAGGACTGTC TTATTCCACT CTTCAGTGAA 
GCTTTACGTT CATGTAAACA GCATGACGTG AGGCCATGGA TGCAGGCATT AAGGTATACT 
ATGTACCAGA ATCAGTTGTT GGAGAAAATT AAAGAACAAA CAGTCCCAAT TAGAAGCCAT 
CTCATGGAAT TAGGTCTAAC AGCAGCAAAA TTTGCTAGAA AACGAGGGAA TGTGTCCCTT 
GCAACAAGAC TGCTGGCACA GTGCAGTGAA GTTCAGCTGG GAAAGACCAC CACTGCACAG 
GATTTAGTCC AACATTTTAA AAAACTATCA ACCCAAGGTC AAGTGGATGA AAAATGGGGG 
CCCGAACTTG ATATTGAAAA AACCAAATTG CTTTATACAG CAGGCCAGTC AACACATGCA 
ATGGAAATGT TGAGTTCTTG TGCCATATCT TTCTGCAAGT CTGTGAAAGC TGAATATGCA 
GTTGCTAAAT CAATTCTGAC ACTGGCTAAA TGGATCCAGG CAGAATGGAA AGAGATTTCA 
GGACAGCTGA AACAGGTTTA CAGAGCTCAG CACCAACAGA ACTTCACAGG TCTTTCTACT 
TTGTCTAAAA ACATACTCAC TCTAATAGAA CTGCCATCTG TTAATACGAT GGAAGAAGAG 
TATCCTCGGA TCGAGAGTGA ATCTACAGTG CATATTGGAG TTGGAGAACC TGACTTCATT 
TTGGGACAGT TGTATCACCT GTCTTCAGTA CAGGCACCTG AAGTAGCCAA ATCTTGGGCA 
GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC AGAAAGGTGG TTGACAATGC CAGTCAGGGA 
GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA TCTGAAGTTC AGAATCTACT TCCAGACACT 
ATAACTGAGG AAGAGAAAGA GAGAATATAT GGTATTCTTG GACAGGCTGT GTGTCGGCCG 
GCGGGGATTC AGGATGAAGA TATAACACTT CAGATAACTG AGAGTGAAGA CAACGAAGAA 
GATGACATGG TTGATGTTAT CTGGCGTCAG TTGATATCAA GCTGCCCATG GCTTTCAGAA 
CTTGATGAAA GTGCAACTGA AGGAGTTATT AAAGTGTGGA GGAAAGTTGT AGATAGAATA 
TTCAGCCTGT ACAAACTCTC TTGCAGTGCA TACTTTACTT TCCTTAAACT CAACGCTGGT 
CAAATTCCTT TAGATGAGGA TGACCCTAGG CTGCATTTAA GTCACAGAGT GGAACAGAGC 
ACTGATGACA TGATTGTGAT GGCCACATTG CGCCTGCTGC GGTTGCTCGT GAAGCATGCT 
GGTGAGCTTC GGCAGTATCT GGAGCACGGC TTGGAGACAA CACCCACTGC ACCATGGAGA 
GGAATTATTC CGCAACTTTT CTCACGCTTA AACCACCCTG AAGTGTATGT GCGCCAAAGT 
ATTTGTAACC TTCTCTGCCG TGTGGCTCAA GATTCCCCAC ATCTCATATT GTATCCTGCA 
ATAGTGGGTA CCATATCGCT TAGTAGTGAA TCCCAGGCTT CAGGAAATAA ATTTTCCACT 
GCAATTCCAA CTTTACTTGG CAATATTCAA GGAGAAGAAT TGCTGGTTTC TGAATGTGAG 
GGAGGAAGTC CTCCTGCATC TCAGGATAGC AATAAGGATG AACCTAAAAG TGGATTAAAT 
GAAGACCAAG CCATGATGCA GGATTGTTAC AGCAAAATTG TAGATAAGCT GTCCTCTGCA 
AACCCCACCA TGGTATTACA GGTTCAGATG CTCGTGGCTG AACTGCGCAG GGTCACTGTG 
CTCTGGGATG AGCTCTGGCT GGGAGTTTTG CTGCAACAAC ACATGTATGT CCTGAGACGA 
ATTCAGCAGC TTGAAGATGA GGTGAAGAGA GTCCAGAACA ACAACACCTT ACGCAAAGAA 
GAGAAAATTG CAATCATGAG GGAGAAGCAC ACAGCTTTGA TGAAGCCCAT CGTATTTGCT 
TTGGAGCATG TGAGGAGTAT CACAGCGGCT CCTGCAGAAA CACCTCATGA AAAATGGTTT 
CAGGATAACT ATGGTGATGC CATTGAAAAT GCCCTAGAAA AACTGAAGAC TCCATTGAAC 
CCTGCAAAGC CTGGGAGCAG CTGGATTCCA TTTAAAGAGA TAATGCTAAG TTTGCAACAG 
AGAGCACAGA AACGTGCAAG TTACATCTTG CGTCTTGAAG AAATCAGTCC ATGGTTGGCT 
GCCATGACTA ACACTGAAAT TGCTCTTCCT GGGGAAGTCT CAGCCAGAGA CACTGTCACA 
ATCCATAGTG TGGGCGGAAC CATCACAATC TTACCGACTA AAACCAAGCC AAAGAAACTT 
CTCTTTCTTG GATCAGATGG GAAGAGCTAT CCTTATCTTT TCAAAGGACT GGAGGATTTA 
CATCTGGATG AGAGAATAAT GCAGTTCCTA TCTATTGTGA ATACCATGTT TGCTACAATT 
AATCGCCAAG AAACACCCCG GTTCCATGCT CGACACTATT CTGTAACACC ACTAGGAACA 
AGATCAGGAC TAATCCAGTG GGTAGATGGA GCCACACCCT TATTTGGTCT TTACAAACGA 
TGGCAACAAC GGGAAGCTGC CTTACAAGCA CAAAAGGCCC AAGATTCCTA CCAAACTCCT 
CAGAATCCTG GAATTGTACC CCGTCCTAGT GAACTTTATT ACAGTAAAAT TGGCCCTGCT 
TTGAAAACAG TTGGGCTTAG CCTGGATGTG TCCCGTCGGG ATTGGCCTCT TCATGTAATG 
AAGGCAGTAT TGGAAGAGTT AATGGAGGCC ACACCCCCGA ATCTCCTTGC CAAAGAGCTC 
TGGTCATCTT GCACAACACC TGATGAATGG TGGAGAGTTA CGCAGTCTTA TGCAAGATCT 
ACTGCAGTCA TGTCTATGGT TGGATACATA ATTGGCCTTG GAGACAGACA TCTGGATAAT 
GTTCTTATAG ATATGACGAC TGGAGAAGTT GTTCACATAG ATTACAATGT TTGCTTTGAA 
AAAGGTAAAA GCCTTAGAGT TCCTGAGAAA GTACCTTTTC GAATGACACA AAACATTGAA 
ACAGCACTGG GTGTAACTGG AGTAGAAGGT GTATTTAGGC TTTCATGTGA GCAGGTTTTA 
CACATTATGC GGCGTGGCAG AGAGACCCTG CTGACGCTGC TGGAGGCCTT TGTGTACGAC 
CCTCTGGTGG ACTGGACAGC AGGAGGCGAG GCTGGGTTTG CTGGTGCTGT CTATGGTGGA 
GGTGGCCAGC AGGCCGAGAG CAAGCAGAGC AAGAGAGAGA TGGAGCGAGA GATCACCCGC 
AGCCTGTTTT CTTCTAGAGT AGCTGAGATT AAGGTGAACT GGTTTAAGAA TAGAGATGAG 
ATGCTGGTTG TGCTTCCCAA GTTGGACGGT AGCTTAGATG AATACCTAAG CTTGCAAGAG 
CAACTGACAG ATGTGGAAAA ACTGCAGGGC AAACTACTGG AGGAAATAGA GTTTCTAGAA 
GGAGCTGAAG GGGTGGATCA TCCTTCTCAT ACTCTGCAAC ACAGGTATTC TGAGCACACC 
CAACTACAGA CTCAGCAAAG AGCTGTTCAG GAAGCAATCC AGGTGAAGCT GAATGAATTT 
GAACAATGGA TAACACATTA TCAGGCTGCA TTCAATAATT TAGAAGCAAC ACAGCTTGCA 
AGCTTGCTTC AAGAGATAAG CACACAAATG GACCTTGGTC CTCCAAGTTA CGTGCCAGCA 
ACAGCCTTTC TGCAGAATGC TGGTCAGGCC CACTTGATTA GCCAGTGCGA GCAGCTGGAG 
GGGGAGGTTG GTGCTCTCCT GCAGCAGAGG CGCTCCGTGC TCCGTGGCTG TCTGGAGCAA 
CTGCATCACT ATGCAACCGT GGCCCTGCAG TATCCGAAGG CCATATTTCA GAAACATCGA 
ATTGAACAGT GGAAGACCTG GATGGAAGAG CTCATCTGTA ACACCACAGT AGAGCGTTGT 
CAAGAGCTCT ATAGGAAATA TGAAATGCAA TATGCTCCCC AGCCACCCCC AACAGTGTGT 
CAGTTCATCA CTGCCACTGA AATGACCCTG CAGCGATACG CAGCAGACAT CAACAGCAGA 
CTTATTAGAC AAGTGGAACG CTTGAAACAG GAAGCTGTCA CTGTGCCAGT TTGTGAAGAT 
CAGTTGAAAG AAATTGAACG TTGCATTAAA GTTTTCCTTC ATGAGAATGG AGAAGAAGGA 
TCTTTGAGTC TAGCAAGTGT TATTATTTCT GCCCTTTGTA CCCTTACAAG GCGTAACCTG 
ATGATGGAAG GTGCAGCGTC AAGTGCTGGA GAACAGCTGG TTGATCTGAC TTCTCGGGAT 
GGAGCCTGGT TCTTGGAGGA ACTCTGCAGT ATGAGCGGAA ACGTCACCTG CTTGGTTCAG 
TTACTGAAGC AGTGCCACCT GGTGCCACAG GACTTAGATA TCCCGAACCC CATGGAAGCG 
TCTGAGACAG TTCACTTAGC CAATGGAGTG TATACCTCAC TTCAGGAATT GAATTCGAAT 
TTCCGGCAAA TCATATTTCC AGAAGCACTT CGATGTTTAA TGAAAGGGGA ATACACGTTA 
GAAAGTATGC TGCATGAACT GGACGGTCTT ATTGAGCAGA CCACCGATGG CGTTCCCCTG 
CAGACTCTAG TGGAATCTCT TCAGGCCTAC TTAAGAAACG CAGCTATGGG ACTGGAAGAA 
GAAACACATG CTCATTACAT CGATGTTGCC AGACTACTAC ATGCTCAGTA CGGTGAATTA 
ATCCAACCGA GAAATGGTTC AGTTGATGAA ACACCCAAAA TGTCAGCTGG CCAGATGCTT 
TTGGTAGCAT TCGATGGCAT GTTTGCTCAA GTTGAAACTG CTTTCAGCTT ATTAGTTGAA 
AAGTTGAACA AGATGGAAAT TCCCATAGCT TGGCGAAAGA TTGACATCAT AAGGGAAGCC 
AGGAGTACTC AAGTTAATTT TTTTGATGAT GATAATCACC GGCAGGTGCT AGAAGAGATT 
TTCTTTCTAA AAAGACTACA GACTATTAAG GAGTTCTTCA GGCTCTGTGG TACCTTTTCT 
AAAACATTGT CAGGATCAAG TTCACTTGAA GATCAGAATA CTGTGAATGG GCCTGTACAG 
ATTGTCAATG TGAAAACCCT TTTTAGAAAC TCTTGTTTCA GTGAAGACCA AATGGCCAAA 
CCTATCAAGG CATTCACAGC TGACTTTGTG AGGCAGCTCT TGATAGGGCT ACCCAACCAA 
GCCCTCGGAC TCACACTGTG CAGTTTTATC AGTGCTCTGG GTGTAGACAT CATTGCTCAA 
GTAGAGGCAA AGGACTTTGG TGCCGAAAGC AAAGTTTCTG TTGATGATCT CTGTAAGAAA 
GCGGTGGAAC ATAACATCCA GATAGGGAAG TTCTCTCAGC TGGTTATGAA CAGGGCAACT 
GTGTTAGCAA GTTCTTACGA CACTGCCTGG AAGAAGCATG ACTTGGTGCG AAGGCTAGAA 
ACCAGTATTT CTTCTTGTAA GACAAGCCTG CAGCGGGTTC AGCTGCATAT TGCCATGTTT 
CAGTGGCAAC ATGAAGATCT ACTTATCAAT AGACCACAAG CCATGTCAGT CACACCTCCC 
CCACGGTCTG CTATCCTAAC CAGCATGAAA AAGAAGCTGC ATACCCTGAG CCAGATTGAA 
ACTTCTATTG CAACAGTTCA GGAGAAGCTA GCTGCACTTG AATCAAGTAT TGAACAGCGA 
CTCAAGTGGG CAGGTGGTGC CAACCCTGCA TTGGCCCCTG TACTACAAGA TTTTGAAGCA 
ACGATAGCTG AAAGAAGAAA TCTTGTCCTT AAAGAGAGCC AAAGAGCAAG TCAGGTCACA 
TTTCTCTGCA GCAATATCAT TCATTTTGAA AGTTTACGAA CAAGAACTGC AGAAGCCTTA 
AACCTGGATG CGGCGTTATT TGAACTAATC AAGCGATGTC AGCAGATGTG TTCGTTTGCA 
TCACAGTTTA ACAGTTCAGT GTCTGAGTTA GAGCTTCGTT TATTACAGAG AGTGGACACT 
GGTCTTGAAC ATCCTATTGG CAGCTCTGAA TGGCTTTTGT CAGCACACAA ACAGTTGACC 
CAGGATATGT CTACTCAGAG GGCAATTCAG ACAGAGAAAG AGCAGCAGAT AGAAACGGTC 
TGTGAAACAA TTCAGAATCT GGTTGATAAT ATAAAGACTG TGCTCACTGG TCATAACCGA 
CAGCTTGGAG ATGTCAAACA TCTCTTGAAA GCTATGGCTA AGGATGAAGA AGCTGCTCTG 
GCAGATGGTG AAGATGTTCC CTATGAGAAC AGTGTTAGGC AGTTTTTGGG TGAATATAAA 
TCATGGCAAG ACAACATTCA AACAGTTCTA TTTACATTAG TCCAGGCTAT GGGTCAGGTT 
CGAAGTCAAG AACACGTTGA AATGCTCCAG GAAATCACTC CCACCTTGAA AGAACTGAAA 
ACACAAAGTC AGAGTATCTA TAATAATTTA GTGAGTTTTG CATCACCCTT AGTCACCGAT 
GCAACAAATG AATGTTCGAG TCCAACGTCA TCTGCTACTT ATCAGCCATC CTTCGCTGCA 
GCAGTCCGGA GTAACACTGG CCAGAAGACT CAGCCTGATG TCATGTCACA GAATGCTAGA 
AAGCTGATCC AGAAAAATCT TGCTACATCA GCTGATACTC CACCAAGCAC CGTTCCAGGA 
ACTGGCAAGA GTGTTGCTTG TAGTCCTAAA AAGGCAGTCA GAGACCCTAA AACTGGGAAA 
GCGGTGCAAG AGAGAAACTC CTATGCAGTG AGTGTGTGGA AGAGAGTGAA AGCCAAGTTA 
GAGGGCCGAG ATGTTGATCC GAATAGGAGG ATGTCAGTTG CTGAACAGGT TGACTATGTC 
ATTAAGGAAG CAACTAATCT AGATAACTTG GCTCAGCTGT ATGAAGGTTG GACAGCCTGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055907.3
CDS	364..11349
Translation	MSRRAPGSRLSSGGGGGGTKYPRSWNDWQPRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSVQHGSRALATKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	NM_015092.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015092.4

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGGGTT AACAATGTGA CCAGCCCTGA GTTCACCAGT 
GTTCAGCATG GCAGTCGTGC TTTAGCCACC AAAGACATGA GGAAATCACA GGAGAGATCG 
ATGTCTTATT CTGATGAGTC TCGACTGTCG AATCTTCTTC GGAGGATCAC CCGGGAAGAC 
GACAGAGACC GAAGATTGGC TACTGTAAAG CAGTTGAAAG AATTTATTCA GCAACCAGAA 
AATAAGCTGG TACTAGTTAA ACAATTGGAT AATATCTTGG CTGCTGTACA TGACGTGCTT 
AATGAAAGTA GCAAATTGCT TCAGGAGTTG AGACAGGAGG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTGAGCTA TGAGGCTGAG AAGATCTTCA AGTGGATTTT TAGCAAATTT 
AGCTCATCTG CAAAAGATGA AGTTAAACTC CTCTACTTAT GTGCCACCTA CAAAGCACTA 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTGTAATGC AGCTTGTAAT GACCAGCCTG 
CAGTCTATTC TTGAAAATGT GGATACACCA GAATTGCTTT GTAAATGTGT TAAGTGCATT 
CTTTTGGTGG CTCGATGTTA CCCTCATATT TTCAGCACTA ATTTTAGGGA TACAGTTGAT 
ATATTAGTTG GATGGCATAT AGATCATACT CAGAAACCTT CGCTCACGCA GCAGGTATCT 
GGGTGGTTGC AGAGTTTGGA GCCATTTTGG GTAGCTGATC TTGCATTTTC TACTACTCTT 
CTTGGTCAGT TTCTGGAAGA CATGGAAGCA TATGCTGAGG ACCTCAGCCA TGTGGCCTCT 
GGGGAATCAG TGGATGAAGA TGTCCCTCCT CCATCAGTGT CATTACCAAA GCTGGCTGCA 
CTTCTCCGGG TATTTAGTAC TGTGGTGAGG AGCATTGGGG AACGCTTCAG CCCAATTCGG 
GGTCCTCCAA TTACTGAGGC ATATGTAACA GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACGGCTGCAA ACCAGGTGTT TTTTTCTGAG GCTGTGTTGA CAGCTGCTAA TGAGTGTGTT 
GGTGTTTTGC TCGGCAGCTT GGATCCTAGC ATGACTATAC ATTGTGACAT GGTCATTACA 
TATGGATTAG ACCAACTGGA GAATTGCCAG ACTTGTGGTA CCGATTATAT CATCTCAGTC 
TTGAATTTAC TCACGCTGAT TGTTGAACAG ATAAATACGA AACTGCCATC ATCATTTGTA 
GAAAAACTGT TTATACCATC ATCTAAACTA CTATTCTTGC GTTATCATAA AGAAAAAGAG 
GTTGTTGCTG TAGCCCATGC TGTTTATCAA GCAGTGCTCA GCTTGAAGAA TATTCCTGTT 
TTGGAGACTG CCTATAAGTT AATATTGGGA GAAATGACTT GTGCCCTAAA CAACCTCCTA 
CACAGTCTAC AACTTCCTGA GGCCTGTTCT GAAATAAAAC ATGAGGCTTT TAAGAATCAT 
GTGTTCAATG TAGACAATGC AAAATTTGTA GTTATATTTG ACCTCAGTGC CCTGACTACA 
ATTGGAAATG CCAAAAACTC ACTAATAGGG ATGTGGGCGC TATCTCCAAC TGTCTTTGCA 
CTTCTGAGTA AGAATCTGAT GATTGTGCAC AGTGACCTGG CTGTTCACTT CCCTGCCATT 
CAGTATGCTG TGCTCTACAC ATTGTATTCT CATTGTACCA GGCATGATCA CTTTATCTCT 
AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG TTTGATGGAG CTGTGATTAG CACTGTAACT 
ACGGCTACAA AGAAACATTT CTCAATTATA TTAAATCTTC TGGGAATATT ACTTAAGAAA 
GATAACCTTA ACCAGGACAC GAGGAAACTG TTAATGACTT GGGCTTTGGA AGCAGCTGTT 
TTAATGAAGA AGTCTGAAAC ATACGCACCT TTATTCTCTC TTCCGTCTTT CCATAAATTT 
TGCAAAGGCC TTTTAGCCAA CACTCTCGTT GAAGATGTGA ATATCTGTCT GCAGGCATGC 
AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG CCAGATGATC TTTTACAGAG ATGTGTCGAT 
GTTTGCCGTG TTCAACTAGT GCACAGTGGA ACTCGTATTC GACAAGCATT TGGAAAACTG 
TTGAAATCAA TTCCTTTAGA TGTTGTCCTA AGCAATAACA ATCACACAGA AATTCAAGAA 
ATTTCTTTAG CATTAAGAAG TCACATGAGT AAAGCACCAA GTAATACATT CCACCCCCAA 
GATTTCTCTG ATGTTATTAG TTTTATTTTG TATGGGAACT CTCATAGAAC AGGGAAGGAC 
AATTGGTTGG AAAGACTGTT CTATAGCTGC CAGAGACTGG ATAAGCGTGA CCAGTCAACA 
ATTCCACGCA ATCTCCTGAA GACAGATGCT GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT 
GCACAATTCA CTGTTCTTTC TAAGCTGAGA ACCCCACTGG GCAGAGCTCA AGACACCTTC 
CAGACAATTG AAGGTATCAT TCGAAGTCTC GCAGCTCACA CATTAAACCC TGATCAGGAT 
GTTAGTCAGT GGACAACTGC AGACAATGAT GAAGGCCATG GTAACAACCA ACTTAGACTT 
GTTCTTCTTC TGCAGTATCT GGAAAATCTG GAGAAATTAA TGTATAATGC ATACGAGGGA 
TGTGCTAATG CATTAACTTC ACCTCCCAAG GTCATTAGAA CTTTTTTCTA TACCAATCGC 
CAAACTTGTC AGGACTGGCT AACGCGGATT CGACTCTCCA TCATGAGGGT AGGATTGTTG 
GCAGGCCAGC CTGCAGTGAC AGTGAGACAT GGCTTTGACT TGCTTACAGA GATGAAAACA 
ACCAGCCTAT CTCAGGGGAA TGAATTGGAA GTAACCATTA TGATGGTGGT AGAAGCATTA 
TGTGAACTTC ATTGTCCTGA AGCTATACAG GGAATTGCTG TCTGGTCATC ATCTATTGTT 
GGAAAAAATC TTCTGTGGAT TAACTCAGTG GCTCAACAGG CTGAAGGGAG GTTTGAAAAG 
GCCTCTGTGG AGTACCAGGA ACACCTGTGT GCCATGACAG GTGTTGATTG CTGCATCTCC 
AGCTTTGACA AATCGGTGCT CACCTTAGCC AATGCTGGGC GTAACAGTGC CAGCCCGAAA 
CATTCTCTGA ATGGTGAATC CAGAAAAACT GTGCTGTCCA AACCGACTGA CTCTTCCCCT 
GAGGTTATAA ATTATTTAGG AAATAAAGCA TGTGAGTGCT ACATCTCAAT TGCCGATTGG 
GCTGCTGTGC AGGAATGGCA GAACGCTATC CATGACTTGA AAAAGAGTAC CAGTAGCACT 
TCCCTCAACC TGAAAGCTGA CTTCAACTAT ATAAAATCAT TAAGCAGCTT TGAGTCTGGA 
AAATTTGTTG AATGTACCGA GCAGTTAGAA TTGTTACCAG GAGAAAATAT CAATCTACTT 
GCTGGAGGAT CAAAAGAAAA AATAGACATG AAAAAACTGC TTCCTAACAT GTTAAGTCCG 
GATCCGAGGG AACTTCAGAA ATCCATTGAA GTTCAATTGT TAAGAAGTTC TGTTTGTTTG 
GCAACTGCTT TAAACCCGAT AGAACAAGAT CAGAAGTGGC AGTCTATAAC TGAAAATGTG 
GTAAAGTACT TGAAGCAAAC ATCCCGCATC GCTATTGGAC CTCTGAGACT TTCTACTTTA 
ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT ACCTTGCAGC TGTATTGCTC ATCTGCTTTG 
GAGAACACAG TTTCTAACAG ACTTTCAACA GAGGACTGTC TTATTCCACT CTTCAGTGAA 
GCTTTACGTT CATGTAAACA GCATGACGTG AGGCCATGGA TGCAGGCATT AAGGTATACT 
ATGTACCAGA ATCAGTTGTT GGAGAAAATT AAAGAACAAA CAGTCCCAAT TAGAAGCCAT 
CTCATGGAAT TAGGTCTAAC AGCAGCAAAA TTTGCTAGAA AACGAGGGAA TGTGTCCCTT 
GCAACAAGAC TGCTGGCACA GTGCAGTGAA GTTCAGCTGG GAAAGACCAC CACTGCACAG 
GATTTAGTCC AACATTTTAA AAAACTATCA ACCCAAGGTC AAGTGGATGA AAAATGGGGG 
CCCGAACTTG ATATTGAAAA AACCAAATTG CTTTATACAG CAGGCCAGTC AACACATGCA 
ATGGAAATGT TGAGTTCTTG TGCCATATCT TTCTGCAAGT CTGTGAAAGC TGAATATGCA 
GTTGCTAAAT CAATTCTGAC ACTGGCTAAA TGGATCCAGG CAGAATGGAA AGAGATTTCA 
GGACAGCTGA AACAGGTTTA CAGAGCTCAG CACCAACAGA ACTTCACAGG TCTTTCTACT 
TTGTCTAAAA ACATACTCAC TCTAATAGAA CTGCCATCTG TTAATACGAT GGAAGAAGAG 
TATCCTCGGA TCGAGAGTGA ATCTACAGTG CATATTGGAG TTGGAGAACC TGACTTCATT 
TTGGGACAGT TGTATCACCT GTCTTCAGTA CAGGCACCTG AAGTAGCCAA ATCTTGGGCA 
GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC AGAAAGGTGG TTGACAATGC CAGTCAGGGA 
GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA TCTGAAGTTC AGAATCTACT TCCAGACACT 
ATAACTGAGG AAGAGAAAGA GAGAATATAT GGTATTCTTG GACAGGCTGT GTGTCGGCCG 
GCGGGGATTC AGGATGAAGA TATAACACTT CAGATAACTG AGAGTGAAGA CAACGAAGAA 
GATGACATGG TTGATGTTAT CTGGCGTCAG TTGATATCAA GCTGCCCATG GCTTTCAGAA 
CTTGATGAAA GTGCAACTGA AGGAGTTATT AAAGTGTGGA GGAAAGTTGT AGATAGAATA 
TTCAGCCTGT ACAAACTCTC TTGCAGTGCA TACTTTACTT TCCTTAAACT CAACGCTGGT 
CAAATTCCTT TAGATGAGGA TGACCCTAGG CTGCATTTAA GTCACAGAGT GGAACAGAGC 
ACTGATGACA TGATTGTGAT GGCCACATTG CGCCTGCTGC GGTTGCTCGT GAAGCATGCT 
GGTGAGCTTC GGCAGTATCT GGAGCACGGC TTGGAGACAA CACCCACTGC ACCATGGAGA 
GGAATTATTC CGCAACTTTT CTCACGCTTA AACCACCCTG AAGTGTATGT GCGCCAAAGT 
ATTTGTAACC TTCTCTGCCG TGTGGCTCAA GATTCCCCAC ATCTCATATT GTATCCTGCA 
ATAGTGGGTA CCATATCGCT TAGTAGTGAA TCCCAGGCTT CAGGAAATAA ATTTTCCACT 
GCAATTCCAA CTTTACTTGG CAATATTCAA GGAGAAGAAT TGCTGGTTTC TGAATGTGAG 
GGAGGAAGTC CTCCTGCATC TCAGGATAGC AATAAGGATG AACCTAAAAG TGGATTAAAT 
GAAGACCAAG CCATGATGCA GGATTGTTAC AGCAAAATTG TAGATAAGCT GTCCTCTGCA 
AACCCCACCA TGGTATTACA GGTTCAGATG CTCGTGGCTG AACTGCGCAG GGTCACTGTG 
CTCTGGGATG AGCTCTGGCT GGGAGTTTTG CTGCAACAAC ACATGTATGT CCTGAGACGA 
ATTCAGCAGC TTGAAGATGA GGTGAAGAGA GTCCAGAACA ACAACACCTT ACGCAAAGAA 
GAGAAAATTG CAATCATGAG GGAGAAGCAC ACAGCTTTGA TGAAGCCCAT CGTATTTGCT 
TTGGAGCATG TGAGGAGTAT CACAGCGGCT CCTGCAGAAA CACCTCATGA AAAATGGTTT 
CAGGATAACT ATGGTGATGC CATTGAAAAT GCCCTAGAAA AACTGAAGAC TCCATTGAAC 
CCTGCAAAGC CTGGGAGCAG CTGGATTCCA TTTAAAGAGA TAATGCTAAG TTTGCAACAG 
AGAGCACAGA AACGTGCAAG TTACATCTTG CGTCTTGAAG AAATCAGTCC ATGGTTGGCT 
GCCATGACTA ACACTGAAAT TGCTCTTCCT GGGGAAGTCT CAGCCAGAGA CACTGTCACA 
ATCCATAGTG TGGGCGGAAC CATCACAATC TTACCGACTA AAACCAAGCC AAAGAAACTT 
CTCTTTCTTG GATCAGATGG GAAGAGCTAT CCTTATCTTT TCAAAGGACT GGAGGATTTA 
CATCTGGATG AGAGAATAAT GCAGTTCCTA TCTATTGTGA ATACCATGTT TGCTACAATT 
AATCGCCAAG AAACACCCCG GTTCCATGCT CGACACTATT CTGTAACACC ACTAGGAACA 
AGATCAGGAC TAATCCAGTG GGTAGATGGA GCCACACCCT TATTTGGTCT TTACAAACGA 
TGGCAACAAC GGGAAGCTGC CTTACAAGCA CAAAAGGCCC AAGATTCCTA CCAAACTCCT 
CAGAATCCTG GAATTGTACC CCGTCCTAGT GAACTTTATT ACAGTAAAAT TGGCCCTGCT 
TTGAAAACAG TTGGGCTTAG CCTGGATGTG TCCCGTCGGG ATTGGCCTCT TCATGTAATG 
AAGGCAGTAT TGGAAGAGTT AATGGAGGCC ACACCCCCGA ATCTCCTTGC CAAAGAGCTC 
TGGTCATCTT GCACAACACC TGATGAATGG TGGAGAGTTA CGCAGTCTTA TGCAAGATCT 
ACTGCAGTCA TGTCTATGGT TGGATACATA ATTGGCCTTG GAGACAGACA TCTGGATAAT 
GTTCTTATAG ATATGACGAC TGGAGAAGTT GTTCACATAG ATTACAATGT TTGCTTTGAA 
AAAGGTAAAA GCCTTAGAGT TCCTGAGAAA GTACCTTTTC GAATGACACA AAACATTGAA 
ACAGCACTGG GTGTAACTGG AGTAGAAGGT GTATTTAGGC TTTCATGTGA GCAGGTTTTA 
CACATTATGC GGCGTGGCAG AGAGACCCTG CTGACGCTGC TGGAGGCCTT TGTGTACGAC 
CCTCTGGTGG ACTGGACAGC AGGAGGCGAG GCTGGGTTTG CTGGTGCTGT CTATGGTGGA 
GGTGGCCAGC AGGCCGAGAG CAAGCAGAGC AAGAGAGAGA TGGAGCGAGA GATCACCCGC 
AGCCTGTTTT CTTCTAGAGT AGCTGAGATT AAGGTGAACT GGTTTAAGAA TAGAGATGAG 
ATGCTGGTTG TGCTTCCCAA GTTGGACGGT AGCTTAGATG AATACCTAAG CTTGCAAGAG 
CAACTGACAG ATGTGGAAAA ACTGCAGGGC AAACTACTGG AGGAAATAGA GTTTCTAGAA 
GGAGCTGAAG GGGTGGATCA TCCTTCTCAT ACTCTGCAAC ACAGGTATTC TGAGCACACC 
CAACTACAGA CTCAGCAAAG AGCTGTTCAG GAAGCAATCC AGGTGAAGCT GAATGAATTT 
GAACAATGGA TAACACATTA TCAGGCTGCA TTCAATAATT TAGAAGCAAC ACAGCTTGCA 
AGCTTGCTTC AAGAGATAAG CACACAAATG GACCTTGGTC CTCCAAGTTA CGTGCCAGCA 
ACAGCCTTTC TGCAGAATGC TGGTCAGGCC CACTTGATTA GCCAGTGCGA GCAGCTGGAG 
GGGGAGGTTG GTGCTCTCCT GCAGCAGAGG CGCTCCGTGC TCCGTGGCTG TCTGGAGCAA 
CTGCATCACT ATGCAACCGT GGCCCTGCAG TATCCGAAGG CCATATTTCA GAAACATCGA 
ATTGAACAGT GGAAGACCTG GATGGAAGAG CTCATCTGTA ACACCACAGT AGAGCGTTGT 
CAAGAGCTCT ATAGGAAATA TGAAATGCAA TATGCTCCCC AGCCACCCCC AACAGTGTGT 
CAGTTCATCA CTGCCACTGA AATGACCCTG CAGCGATACG CAGCAGACAT CAACAGCAGA 
CTTATTAGAC AAGTGGAACG CTTGAAACAG GAAGCTGTCA CTGTGCCAGT TTGTGAAGAT 
CAGTTGAAAG AAATTGAACG TTGCATTAAA GTTTTCCTTC ATGAGAATGG AGAAGAAGGA 
TCTTTGAGTC TAGCAAGTGT TATTATTTCT GCCCTTTGTA CCCTTACAAG GCGTAACCTG 
ATGATGGAAG GTGCAGCGTC AAGTGCTGGA GAACAGCTGG TTGATCTGAC TTCTCGGGAT 
GGAGCCTGGT TCTTGGAGGA ACTCTGCAGT ATGAGCGGAA ACGTCACCTG CTTGGTTCAG 
TTACTGAAGC AGTGCCACCT GGTGCCACAG GACTTAGATA TCCCGAACCC CATGGAAGCG 
TCTGAGACAG TTCACTTAGC CAATGGAGTG TATACCTCAC TTCAGGAATT GAATTCGAAT 
TTCCGGCAAA TCATATTTCC AGAAGCACTT CGATGTTTAA TGAAAGGGGA ATACACGTTA 
GAAAGTATGC TGCATGAACT GGACGGTCTT ATTGAGCAGA CCACCGATGG CGTTCCCCTG 
CAGACTCTAG TGGAATCTCT TCAGGCCTAC TTAAGAAACG CAGCTATGGG ACTGGAAGAA 
GAAACACATG CTCATTACAT CGATGTTGCC AGACTACTAC ATGCTCAGTA CGGTGAATTA 
ATCCAACCGA GAAATGGTTC AGTTGATGAA ACACCCAAAA TGTCAGCTGG CCAGATGCTT 
TTGGTAGCAT TCGATGGCAT GTTTGCTCAA GTTGAAACTG CTTTCAGCTT ATTAGTTGAA 
AAGTTGAACA AGATGGAAAT TCCCATAGCT TGGCGAAAGA TTGACATCAT AAGGGAAGCC 
AGGAGTACTC AAGTTAATTT TTTTGATGAT GATAATCACC GGCAGGTGCT AGAAGAGATT 
TTCTTTCTAA AAAGACTACA GACTATTAAG GAGTTCTTCA GGCTCTGTGG TACCTTTTCT 
AAAACATTGT CAGGATCAAG TTCACTTGAA GATCAGAATA CTGTGAATGG GCCTGTACAG 
ATTGTCAATG TGAAAACCCT TTTTAGAAAC TCTTGTTTCA GTGAAGACCA AATGGCCAAA 
CCTATCAAGG CATTCACAGC TGACTTTGTG AGGCAGCTCT TGATAGGGCT ACCCAACCAA 
GCCCTCGGAC TCACACTGTG CAGTTTTATC AGTGCTCTGG GTGTAGACAT CATTGCTCAA 
GTAGAGGCAA AGGACTTTGG TGCCGAAAGC AAAGTTTCTG TTGATGATCT CTGTAAGAAA 
GCGGTGGAAC ATAACATCCA GATAGGGAAG TTCTCTCAGC TGGTTATGAA CAGGGCAACT 
GTGTTAGCAA GTTCTTACGA CACTGCCTGG AAGAAGCATG ACTTGGTGCG AAGGCTAGAA 
ACCAGTATTT CTTCTTGTAA GACAAGCCTG CAGCGGGTTC AGCTGCATAT TGCCATGTTT 
CAGTGGCAAC ATGAAGATCT ACTTATCAAT AGACCACAAG CCATGTCAGT CACACCTCCC 
CCACGGTCTG CTATCCTAAC CAGCATGAAA AAGAAGCTGC ATACCCTGAG CCAGATTGAA 
ACTTCTATTG CAACAGTTCA GGAGAAGCTA GCTGCACTTG AATCAAGTAT TGAACAGCGA 
CTCAAGTGGG CAGGTGGTGC CAACCCTGCA TTGGCCCCTG TACTACAAGA TTTTGAAGCA 
ACGATAGCTG AAAGAAGAAA TCTTGTCCTT AAAGAGAGCC AAAGAGCAAG TCAGGTCACA 
TTTCTCTGCA GCAATATCAT TCATTTTGAA AGTTTACGAA CAAGAACTGC AGAAGCCTTA 
AACCTGGATG CGGCGTTATT TGAACTAATC AAGCGATGTC AGCAGATGTG TTCGTTTGCA 
TCACAGTTTA ACAGTTCAGT GTCTGAGTTA GAGCTTCGTT TATTACAGAG AGTGGACACT 
GGTCTTGAAC ATCCTATTGG CAGCTCTGAA TGGCTTTTGT CAGCACACAA ACAGTTGACC 
CAGGATATGT CTACTCAGAG GGCAATTCAG ACAGAGAAAG AGCAGCAGAT AGAAACGGTC 
TGTGAAACAA TTCAGAATCT GGTTGATAAT ATAAAGACTG TGCTCACTGG TCATAACCGA 
CAGCTTGGAG ATGTCAAACA TCTCTTGAAA GCTATGGCTA AGGATGAAGA AGCTGCTCTG 
GCAGATGGTG AAGATGTTCC CTATGAGAAC AGTGTTAGGC AGTTTTTGGG TGAATATAAA 
TCATGGCAAG ACAACATTCA AACAGTTCTA TTTACATTAG TCCAGGCTAT GGGTCAGGTT 
CGAAGTCAAG AACACGTTGA AATGCTCCAG GAAATCACTC CCACCTTGAA AGAACTGAAA 
ACACAAAGTC AGAGTATCTA TAATAATTTA GTGAGTTTTG CATCACCCTT AGTCACCGAT 
GCAACAAATG AATGTTCGAG TCCAACGTCA TCTGCTACTT ATCAGCCATC CTTCGCTGCA 
GCAGTCCGGA GTAACACTGG CCAGAAGACT CAGCCTGATG TCATGTCACA GAATGCTAGA 
AAGCTGATCC AGAAAAATCT TGCTACATCA GCTGATACTC CACCAAGCAC CGTTCCAGGA 
ACTGGCAAGA GTGTTGCTTG TAGTCCTAAA AAGGCAGTCA GAGACCCTAA AACTGGGAAA 
GCGGTGCAAG AGAGAAACTC CTATGCAGTG AGTGTGTGGA AGAGAGTGAA AGCCAAGTTA 
GAGGGCCGAG ATGTTGATCC GAATAGGAGG ATGTCAGTTG CTGAACAGGT TGACTATGTC 
ATTAAGGAAG CAACTAATCT AGATAACTTG GCTCAGCTGT ATGAAGGTTG GACAGCCTGG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_024450199.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305967.1 Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X7, mRNA.

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CloneID	OHu104104
Clone ID Related Accession (Same CDS sequence)	XM_024450199.1 , XM_024450198.1
Accession Version	XM_024450199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATAATTTAAA ATATTCTTCA 
TCCAGAGATA GAGGTGGTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACGCTGACA TACAGAATGA CGAAAAGGAG 
AGATCGATGT CTTATTCTGA TGAGTCTCGA CTGTCGAATC TTCTTCGGAG GATCACCCGG 
GAAGACGACA GAGACCGAAG ATTGGCTACT GTAAAGCAGT TGAAAGAATT TATTCAGCAA 
CCAGAAAATA AGCTGGTACT AGTTAAACAA TTGGATAATA TCTTGGCTGC TGTACATGAC 
GTGCTTAATG AAAGTAGCAA ATTGCTTCAG GAGTTGAGAC AGGAGGGAGC TTGCTGTCTT 
GGCCTTCTTT GTGCTTCTCT GAGCTATGAG GCTGAGAAGA TCTTCAAGTG GATTTTTAGC 
AAATTTAGCT CATCTGCAAA AGATGAAGTT AAACTCCTCT ACTTATGTGC CACCTACAAA 
GCACTAGAGA CTGTAGGAGA AAAGAAAGCC TTTTCATCTG TAATGCAGCT TGTAATGACC 
AGCCTGCAGT CTATTCTTGA AAATGTGGAT ACACCAGAAT TGCTTTGTAA ATGTGTTAAG 
TGCATTCTTT TGGTGGCTCG ATGTTACCCT CATATTTTCA GCACTAATTT TAGGGATACA 
GTTGATATAT TAGTTGGATG GCATATAGAT CATACTCAGA AACCTTCGCT CACGCAGCAG 
GTATCTGGGT GGTTGCAGAG TTTGGAGCCA TTTTGGGTAG CTGATCTTGC ATTTTCTACT 
ACTCTTCTTG GTCAGTTTCT GGAAGACATG GAAGCATATG CTGAGGACCT CAGCCATGTG 
GCCTCTGGGG AATCAGTGGA TGAAGATGTC CCTCCTCCAT CAGTGTCATT ACCAAAGCTG 
GCTGCACTTC TCCGGGTATT TAGTACTGTG GTGAGGAGCA TTGGGGAACG CTTCAGCCCA 
ATTCGGGGTC CTCCAATTAC TGAGGCATAT GTAACAGATG TTCTGTACAG AGTAATGAGA 
TGTGTGACGG CTGCAAACCA GGTGTTTTTT TCTGAGGCTG TGTTGACAGC TGCTAATGAG 
TGTGTTGGTG TTTTGCTCGG CAGCTTGGAT CCTAGCATGA CTATACATTG TGACATGGTC 
ATTACATATG GATTAGACCA ACTGGAGAAT TGCCAGACTT GTGGTACCGA TTATATCATC 
TCAGTCTTGA ATTTACTCAC GCTGATTGTT GAACAGATAA ATACGAAACT GCCATCATCA 
TTTGTAGAAA AACTGTTTAT ACCATCATCT AAACTACTAT TCTTGCGTTA TCATAAAGAA 
AAAGAGGTTG TTGCTGTAGC CCATGCTGTT TATCAAGCAG TGCTCAGCTT GAAGAATATT 
CCTGTTTTGG AGACTGCCTA TAAGTTAATA TTGGGAGAAA TGACTTGTGC CCTAAACAAC 
CTCCTACACA GTCTACAACT TCCTGAGGCC TGTTCTGAAA TAAAACATGA GGCTTTTAAG 
AATCATGTGT TCAATGTAGA CAATGCAAAA TTTGTAGTTA TATTTGACCT CAGTGCCCTG 
ACTACAATTG GAAATGCCAA AAACTCACTA ATAGGGATGT GGGCGCTATC TCCAACTGTC 
TTTGCACTTC TGAGTAAGAA TCTGATGATT GTGCACAGTG ACCTGGCTGT TCACTTCCCT 
GCCATTCAGT ATGCTGTGCT CTACACATTG TATTCTCATT GTACCAGGCA TGATCACTTT 
ATCTCTAGTA GCCTCAGTTC TTCCTCTCCT TCTTTGTTTG ATGGAGCTGT GATTAGCACT 
GTAACTACGG CTACAAAGAA ACATTTCTCA ATTATATTAA ATCTTCTGGG AATATTACTT 
AAGAAAGATA ACCTTAACCA GGACACGAGG AAACTGTTAA TGACTTGGGC TTTGGAAGCA 
GCTGTTTTAA TGAAGAAGTC TGAAACATAC GCACCTTTAT TCTCTCTTCC GTCTTTCCAT 
AAATTTTGCA AAGGCCTTTT AGCCAACACT CTCGTTGAAG ATGTGAATAT CTGTCTGCAG 
GCATGCAGCA GTCTACATGC TCTGTCCTCT TCCTTGCCAG ATGATCTTTT ACAGAGATGT 
GTCGATGTTT GCCGTGTTCA ACTAGTGCAC AGTGGAACTC GTATTCGACA AGCATTTGGA 
AAACTGTTGA AATCAATTCC TTTAGATGTT GTCCTAAGCA ATAACAATCA CACAGAAATT 
CAAGAAATTT CTTTAGCATT AAGAAGTCAC ATGAGTAAAG CACCAAGTAA TACATTCCAC 
CCCCAAGATT TCTCTGATGT TATTAGTTTT ATTTTGTATG GGAACTCTCA TAGAACAGGG 
AAGGACAATT GGTTGGAAAG ACTGTTCTAT AGCTGCCAGA GACTGGATAA GCGTGACCAG 
TCAACAATTC CACGCAATCT CCTGAAGACA GATGCTGTCC TTTGGCAGTG GGCCATATGG 
GAAGCTGCAC AATTCACTGT TCTTTCTAAG CTGAGAACCC CACTGGGCAG AGCTCAAGAC 
ACCTTCCAGA CAATTGAAGG TATCATTCGA AGTCTCGCAG CTCACACATT AAACCCTGAT 
CAGGATGTTA GTCAGTGGAC AACTGCAGAC AATGATGAAG GCCATGGTAA CAACCAACTT 
AGACTTGTTC TTCTTCTGCA GTATCTGGAA AATCTGGAGA AATTAATGTA TAATGCATAC 
GAGGGATGTG CTAATGCATT AACTTCACCT CCCAAGGTCA TTAGAACTTT TTTCTATACC 
AATCGCCAAA CTTGTCAGGA CTGGCTAACG CGGATTCGAC TCTCCATCAT GAGGGTAGGA 
TTGTTGGCAG GCCAGCCTGC AGTGACAGTG AGACATGGCT TTGACTTGCT TACAGAGATG 
AAAACAACCA GCCTATCTCA GGGGAATGAA TTGGAAGTAA CCATTATGAT GGTGGTAGAA 
GCATTATGTG AACTTCATTG TCCTGAAGCT ATACAGGGAA TTGCTGTCTG GTCATCATCT 
ATTGTTGGAA AAAATCTTCT GTGGATTAAC TCAGTGGCTC AACAGGCTGA AGGGAGGTTT 
GAAAAGGCCT CTGTGGAGTA CCAGGAACAC CTGTGTGCCA TGACAGGTGT TGATTGCTGC 
ATCTCCAGCT TTGACAAATC GGTGCTCACC TTAGCCAATG CTGGGCGTAA CAGTGCCAGC 
CCGAAACATT CTCTGAATGG TGAATCCAGA AAAACTGTGC TGTCCAAACC GACTGACTCT 
TCCCCTGAGG TTATAAATTA TTTAGGAAAT AAAGCATGTG AGTGCTACAT CTCAATTGCC 
GATTGGGCTG CTGTGCAGGA ATGGCAGAAC GCTATCCATG ACTTGAAAAA GAGTACCAGT 
AGCACTTCCC TCAACCTGAA AGCTGACTTC AACTATATAA AATCATTAAG CAGCTTTGAG 
TCTGGAAAAT TTGTTGAATG TACCGAGCAG TTAGAATTGT TACCAGGAGA AAATATCAAT 
CTACTTGCTG GAGGATCAAA AGAAAAAATA GACATGAAAA AACTGCTTCC TAACATGTTA 
AGTCCGGATC CGAGGGAACT TCAGAAATCC ATTGAAGTTC AATTGTTAAG AAGTTCTGTT 
TGTTTGGCAA CTGCTTTAAA CCCGATAGAA CAAGATCAGA AGTGGCAGTC TATAACTGAA 
AATGTGGTAA AGTACTTGAA GCAAACATCC CGCATCGCTA TTGGACCTCT GAGACTTTCT 
ACTTTAACAG TTTCACAGTC TTTGCCAGTT CTAAGTACCT TGCAGCTGTA TTGCTCATCT 
GCTTTGGAGA ACACAGTTTC TAACAGACTT TCAACAGAGG ACTGTCTTAT TCCACTCTTC 
AGTGAAGCTT TACGTTCATG TAAACAGCAT GACGTGAGGC CATGGATGCA GGCATTAAGG 
TATACTATGT ACCAGAATCA GTTGTTGGAG AAAATTAAAG AACAAACAGT CCCAATTAGA 
AGCCATCTCA TGGAATTAGG TCTAACAGCA GCAAAATTTG CTAGAAAACG AGGGAATGTG 
TCCCTTGCAA CAAGACTGCT GGCACAGTGC AGTGAAGTTC AGCTGGGAAA GACCACCACT 
GCACAGGATT TAGTCCAACA TTTTAAAAAA CTATCAACCC AAGGTCAAGT GGATGAAAAA 
TGGGGGCCCG AACTTGATAT TGAAAAAACC AAATTGCTTT ATACAGCAGG CCAGTCAACA 
CATGCAATGG AAATGTTGAG TTCTTGTGCC ATATCTTTCT GCAAGTCTGT GAAAGCTGAA 
TATGCAGTTG CTAAATCAAT TCTGACACTG GCTAAATGGA TCCAGGCAGA ATGGAAAGAG 
ATTTCAGGAC AGCTGAAACA GGTTTACAGA GCTCAGCACC AACAGAACTT CACAGGTCTT 
TCTACTTTGT CTAAAAACAT ACTCACTCTA ATAGAACTGC CATCTGTTAA TACGATGGAA 
GAAGAGTATC CTCGGATCGA GAGTGAATCT ACAGTGCATA TTGGAGTTGG AGAACCTGAC 
TTCATTTTGG GACAGTTGTA TCACCTGTCT TCAGTACAGG CACCTGAAGT AGCCAAATCT 
TGGGCAGCGT TGGCCAGCTG GGCTTATAGG TGGGGCAGAA AGGTGGTTGA CAATGCCAGT 
CAGGGAGAAG GTGTTCGTCT GCTGCCTAGA GAAAAATCTG AAGTTCAGAA TCTACTTCCA 
GACACTATAA CTGAGGAAGA GAAAGAGAGA ATATATGGTA TTCTTGGACA GGCTGTGTGT 
CGGCCGGCGG GGATTCAGGA TGAAGATATA ACACTTCAGA TAACTGAGAG TGAAGACAAC 
GAAGAAGATG ACATGGTTGA TGTTATCTGG CGTCAGTTGA TATCAAGCTG CCCATGGCTT 
TCAGAACTTG ATGAAAGTGC AACTGAAGGA GTTATTAAAG TGTGGAGGAA AGTTGTAGAT 
AGAATATTCA GCCTGTACAA ACTCTCTTGC AGTGCATACT TTACTTTCCT TAAACTCAAC 
GCTGGTCAAA TTCCTTTAGA TGAGGATGAC CCTAGGCTGC ATTTAAGTCA CAGAGTGGAA 
CAGAGCACTG ATGACATGAT TGTGATGGCC ACATTGCGCC TGCTGCGGTT GCTCGTGAAG 
CATGCTGGTG AGCTTCGGCA GTATCTGGAG CACGGCTTGG AGACAACACC CACTGCACCA 
TGGAGAGGAA TTATTCCGCA ACTTTTCTCA CGCTTAAACC ACCCTGAAGT GTATGTGCGC 
CAAAGTATTT GTAACCTTCT CTGCCGTGTG GCTCAAGATT CCCCACATCT CATATTGTAT 
CCTGCAATAG TGGGTACCAT ATCGCTTAGT AGTGAATCCC AGGCTTCAGG AAATAAATTT 
TCCACTGCAA TTCCAACTTT ACTTGGCAAT ATTCAAGGAG AAGAATTGCT GGTTTCTGAA 
TGTGAGGGAG GAAGTCCTCC TGCATCTCAG GATAGCAATA AGGATGAACC TAAAAGTGGA 
TTAAATGAAG ACCAAGCCAT GATGCAGGAT TGTTACAGCA AAATTGTAGA TAAGCTGTCC 
TCTGCAAACC CCACCATGGT ATTACAGGTT CAGATGCTCG TGGCTGAACT GCGCAGGGTC 
ACTGTGCTCT GGGATGAGCT CTGGCTGGGA GTTTTGCTGC AACAACACAT GTATGTCCTG 
AGACGAATTC AGCAGCTTGA AGATGAGGTG AAGAGAGTCC AGAACAACAA CACCTTACGC 
AAAGAAGAGA AAATTGCAAT CATGAGGGAG AAGCACACAG CTTTGATGAA GCCCATCGTA 
TTTGCTTTGG AGCATGTGAG GAGTATCACA GCGGCTCCTG CAGAAACACC TCATGAAAAA 
TGGTTTCAGG ATAACTATGG TGATGCCATT GAAAATGCCC TAGAAAAACT GAAGACTCCA 
TTGAACCCTG CAAAGCCTGG GAGCAGCTGG ATTCCATTTA AAGAGATAAT GCTAAGTTTG 
CAACAGAGAG CACAGAAACG TGCAAGTTAC ATCTTGCGTC TTGAAGAAAT CAGTCCATGG 
TTGGCTGCCA TGACTAACAC TGAAATTGCT CTTCCTGGGG AAGTCTCAGC CAGAGACACT 
GTCACAATCC ATAGTGTGGG CGGAACCATC ACAATCTTAC CGACTAAAAC CAAGCCAAAG 
AAACTTCTCT TTCTTGGATC AGATGGGAAG AGCTATCCTT ATCTTTTCAA AGGACTGGAG 
GATTTACATC TGGATGAGAG AATAATGCAG TTCCTATCTA TTGTGAATAC CATGTTTGCT 
ACAATTAATC GCCAAGAAAC ACCCCGGTTC CATGCTCGAC ACTATTCTGT AACACCACTA 
GGAACAAGAT CAGGACTAAT CCAGTGGGTA GATGGAGCCA CACCCTTATT TGGTCTTTAC 
AAACGATGGC AACAACGGGA AGCTGCCTTA CAAGCACAAA AGGCCCAAGA TTCCTACCAA 
ACTCCTCAGA ATCCTGGAAT TGTACCCCGT CCTAGTGAAC TTTATTACAG TAAAATTGGC 
CCTGCTTTGA AAACAGTTGG GCTTAGCCTG GATGTGTCCC GTCGGGATTG GCCTCTTCAT 
GTAATGAAGG CAGTATTGGA AGAGTTAATG GAGGCCACAC CCCCGAATCT CCTTGCCAAA 
GAGCTCTGGT CATCTTGCAC AACACCTGAT GAATGGTGGA GAGTTACGCA GTCTTATGCA 
AGATCTACTG CAGTCATGTC TATGGTTGGA TACATAATTG GCCTTGGAGA CAGACATCTG 
GATAATGTTC TTATAGATAT GACGACTGGA GAAGTTGTTC ACATAGATTA CAATGTTTGC 
TTTGAAAAAG GTAATATAAA AGTAGACTTT ACATTTATGA GGGCCAAATG TTTTTTGGTG 
AAAGGGCAAA AATATAATGA TTTGTCTTTT CAAGGTAAAA GCCTTAGAGT TCCTGAGAAA 
GTACCTTTTC GAATGACACA AAACATTGAA ACAGCACTGG GTGTAACTGG AGTAGAAGGT 
GTATTTAGGC TTTCATGTGA GCAGGTTTTA CACATTATGC GGCGTGGCAG AGAGACCCTG 
CTGACGCTGC TGGAGGCCTT TGTGTACGAC CCTCTGGTGG ACTGGACAGC AGGAGGCGAG 
GCTGGGTTTG CTGGTGCTGT CTATGGTGGA GGTGGCCAGC AGGCCGAGAG CAAGCAGAGC 
AAGAGAGAGA TGGAGCGAGA GATCACCCGC AGCCTGTTTT CTTCTAGAGT AGCTGAGATT 
AAGGTGAACT GGTTTAAGAA TAGAGATGAG ATGCTGGTTG TGCTTCCCAA GTTGGACGGT 
AGCTTAGATG AATACCTAAG CTTGCAAGAG CAACTGACAG ATGTGGAAAA ACTGCAGGGC 
AAACTACTGG AGGAAATAGA GTTTCTAGAA GGAGCTGAAG GGGTGGATCA TCCTTCTCAT 
ACTCTGCAAC ACAGGTATTC TGAGCACACC CAACTACAGA CTCAGCAAAG AGCTGTTCAG 
GAAGCAATCC AGGTGAAGCT GAATGAATTT GAACAATGGA TAACACATTA TCAGGCTGCA 
TTCAATAATT TAGAAGCAAC ACAGCTTGCA AGCTTGCTTC AAGAGATAAG CACACAAATG 
GACCTTGGTC CTCCAAGTTA CGTGCCAGCA ACAGCCTTTC TGCAGAATGC TGGTCAGGCC 
CACTTGATTA GCCAGTGCGA GCAGCTGGAG GGGGAGGTTG GTGCTCTCCT GCAGCAGAGG 
CGCTCCGTGC TCCGTGGCTG TCTGGAGCAA CTGCATCACT ATGCAACCGT GGCCCTGCAG 
TATCCGAAGG CCATATTTCA GAAACATCGA ATTGAACAGT GGAAGACCTG GATGGAAGAG 
CTCATCTGTA ACACCACAGT AGAGCGTTGT CAAGAGCTCT ATAGGAAATA TGAAATGCAA 
TATGCTCCCC AGCCACCCCC AACAGTGTGT CAGTTCATCA CTGCCACTGA AATGACCCTG 
CAGCGATACG CAGCAGACAT CAACAGCAGA CTTATTAGAC AAGTGGAACG CTTGAAACAG 
GAAGCTGTCA CTGTGCCAGT TTGTGAAGAT CAGTTGAAAG AAATTGAACG TTGCATTAAA 
GTTTTCCTTC ATGAGAATGG AGAAGAAGGA TCTTTGAGTC TAGCAAGTGT TATTATTTCT 
GCCCTTTGTA CCCTTACAAG GCGTAACCTG ATGATGGAAG GTGCAGCGTC AAGTGCTGGA 
GAACAGCTGG TTGATCTGAC TTCTCGGGAT GGAGCCTGGT TCTTGGAGGA ACTCTGCAGT 
ATGAGCGGAA ACGTCACCTG CTTGGTTCAG TTACTGAAGC AGTGCCACCT GGTGCCACAG 
GACTTAGATA TCCCGAACCC CATGGAAGCG TCTGAGACAG TTCACTTAGC CAATGGAGTG 
TATACCTCAC TTCAGGAATT GAATTCGAAT TTCCGGCAAA TCATATTTCC AGAAGCACTT 
CGATGTTTAA TGAAAGGGGA ATACACGTTA GAAAGTATGC TGCATGAACT GGACGGTCTT 
ATTGAGCAGA CCACCGATGG CGTTCCCCTG CAGACTCTAG TGGAATCTCT TCAGGCCTAC 
TTAAGAAACG CAGCTATGGG ACTGGAAGAA GAAACACATG CTCATTACAT CGATGTTGCC 
AGACTACTAC ATGCTCAGTA CGGTGAATTA ATCCAACCGA GAAATGGTTC AGTTGATGAA 
ACACCCAAAA TGTCAGCTGG CCAGATGCTT TTGGTAGCAT TCGATGGCAT GTTTGCTCAA 
GTTGAAACTG CTTTCAGCTT ATTAGTTGAA AAGTTGAACA AGATGGAAAT TCCCATAGCT 
TGGCGAAAGA TTGACATCAT AAGGGAAGCC AGGAGTACTC AAGTTAATTT TTTTGATGAT 
GATAATCACC GGCAGGTGCT AGAAGAGATT TTCTTTCTAA AAAGACTACA GACTATTAAG 
GAGTTCTTCA GGCTCTGTGG TACCTTTTCT AAAACATTGT CAGGATCAAG TTCACTTGAA 
GATCAGAATA CTGTGAATGG GCCTGTACAG ATTGTCAATG TGAAAACCCT TTTTAGAAAC 
TCTTGTTTCA GTGAAGACCA AATGGCCAAA CCTATCAAGG CATTCACAGC TGACTTTGTG 
AGGCAGCTCT TGATAGGGCT ACCCAACCAA GCCCTCGGAC TCACACTGTG CAGTTTTATC 
AGTGCTCTGG GTGTAGACAT CATTGCTCAA GTAGAGGCAA AGGACTTTGG TGCCGAAAGC 
AAAGTTTCTG TTGATGATCT CTGTAAGAAA GCGGTGGAAC ATAACATCCA GATAGGGAAG 
TTCTCTCAGC TGGTTATGAA CAGGGCAACT GTGTTAGCAA GTTCTTACGA CACTGCCTGG 
AAGAAGCATG ACTTGGTGCG AAGGCTAGAA ACCAGTATTT CTTCTTGTAA GACAAGCCTG 
CAGCGGGTTC AGCTGCATAT TGCCATGTTT CAGTGGCAAC ATGAAGATCT ACTTATCAAT 
AGACCACAAG CCATGTCAGT CACACCTCCC CCACGGTCTG CTATCCTAAC CAGCATGAAA 
AAGAAGCTGC ATACCCTGAG CCAGATTGAA ACTTCTATTG CAACAGTTCA GGAGAAGCTA 
GCTGCACTTG AATCAAGTAT TGAACAGCGA CTCAAGTGGG CAGGTGGTGC CAACCCTGCA 
TTGGCCCCTG TACTACAAGA TTTTGAAGCA ACGATAGCTG AAAGAAGAAA TCTTGTCCTT 
AAAGAGAGCC AAAGAGCAAG TCAGGTCACA TTTCTCTGCA GCAATATCAT TCATTTTGAA 
AGTTTACGAA CAAGAACTGC AGAAGCCTTA AACCTGGATG CGGCGTTATT TGAACTAATC 
AAGCGATGTC AGCAGATGTG TTCGTTTGCA TCACAGTTTA ACAGTTCAGT GTCTGAGTTA 
GAGCTTCGTT TATTACAGAG AGTGGACACT GGTCTTGAAC ATCCTATTGG CAGCTCTGAA 
TGGCTTTTGT CAGCACACAA ACAGTTGACC CAGGATATGT CTACTCAGAG GGCAATTCAG 
ACAGAGAAAG AGCAGCAGAT AGAAACGGTC TGTGAAACAA TTCAGAATCT GGTTGATAAT 
ATAAAGACTG TGCTCACTGG TCATAACCGA CAGCTTGGAG ATGTCAAACA TCTCTTGAAA 
GCTATGGCTA AGGATGAAGA AGCTGCTCTG GCAGATGGTG AAGATGTTCC CTATGAGAAC 
AGTGTTAGGC AGTTTTTGGG TGAATATAAA TCATGGCAAG ACAACATTCA AACAGTTCTA 
TTTACATTAG TCCAGGCTAT GGGTCAGGTT CGAAGTCAAG AACACGTTGA AATGCTCCAG 
GAAATCACTC CCACCTTGAA AGAACTGAAA ACACAAAGTC AGAGTATCTA TAATAATTTA 
GTGAGTTTTG CATCACCCTT AGTCACCGAT GCAACAAATG AATGTTCGAG TCCAACGTCA 
TCTGCTACTT ATCAGCCATC CTTCGCTGCA GCAGTCCGGA GTAACACTGG CCAGAAGACT 
CAGCCTGATG TCATGTCACA GAATGCTAGA AAGCTGATCC AAAAAAATCT TGCTACATCA 
GCTGATACTC CACCAAGCAC CGTTCCAGGA ACTGGCAAGA GTGTTGCTTG TAGTCCTAAA 
AAGGCAGTCA GAGACCCTAA AACTGGGAAA GCGGTGCAAG AGAGAAACTC CTATGCAGTG 
AGTGTGTGGA AGAGAGTGAA AGCCAAGTTA GAGGGCCGAG ATGTTGATCC GAATAGGAGG 
ATGTCAGTTG CTGAACAGGT TGACTATGTC ATTAAGGAAG CAACTAATCT AGATAACTTG 
GCTCAGCTGT ATGAAGGTTG GACAGCCTGG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305967.1
CDS	158..10993
Translation	MRFLRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGNIKVDFTFMRAKCFLVKGQKYNDLSFQGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_024450199.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450199.1

ORF Insert Sequence:

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATAATTTAAA ATATTCTTCA 
TCCAGAGATA GAGGTGGTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACGCTGACA TACAGAATGA CGAAAAGGAG 
AGATCGATGT CTTATTCTGA TGAGTCTCGA CTGTCGAATC TTCTTCGGAG GATCACCCGG 
GAAGACGACA GAGACCGAAG ATTGGCTACT GTAAAGCAGT TGAAAGAATT TATTCAGCAA 
CCAGAAAATA AGCTGGTACT AGTTAAACAA TTGGATAATA TCTTGGCTGC TGTACATGAC 
GTGCTTAATG AAAGTAGCAA ATTGCTTCAG GAGTTGAGAC AGGAGGGAGC TTGCTGTCTT 
GGCCTTCTTT GTGCTTCTCT GAGCTATGAG GCTGAGAAGA TCTTCAAGTG GATTTTTAGC 
AAATTTAGCT CATCTGCAAA AGATGAAGTT AAACTCCTCT ACTTATGTGC CACCTACAAA 
GCACTAGAGA CTGTAGGAGA AAAGAAAGCC TTTTCATCTG TAATGCAGCT TGTAATGACC 
AGCCTGCAGT CTATTCTTGA AAATGTGGAT ACACCAGAAT TGCTTTGTAA ATGTGTTAAG 
TGCATTCTTT TGGTGGCTCG ATGTTACCCT CATATTTTCA GCACTAATTT TAGGGATACA 
GTTGATATAT TAGTTGGATG GCATATAGAT CATACTCAGA AACCTTCGCT CACGCAGCAG 
GTATCTGGGT GGTTGCAGAG TTTGGAGCCA TTTTGGGTAG CTGATCTTGC ATTTTCTACT 
ACTCTTCTTG GTCAGTTTCT GGAAGACATG GAAGCATATG CTGAGGACCT CAGCCATGTG 
GCCTCTGGGG AATCAGTGGA TGAAGATGTC CCTCCTCCAT CAGTGTCATT ACCAAAGCTG 
GCTGCACTTC TCCGGGTATT TAGTACTGTG GTGAGGAGCA TTGGGGAACG CTTCAGCCCA 
ATTCGGGGTC CTCCAATTAC TGAGGCATAT GTAACAGATG TTCTGTACAG AGTAATGAGA 
TGTGTGACGG CTGCAAACCA GGTGTTTTTT TCTGAGGCTG TGTTGACAGC TGCTAATGAG 
TGTGTTGGTG TTTTGCTCGG CAGCTTGGAT CCTAGCATGA CTATACATTG TGACATGGTC 
ATTACATATG GATTAGACCA ACTGGAGAAT TGCCAGACTT GTGGTACCGA TTATATCATC 
TCAGTCTTGA ATTTACTCAC GCTGATTGTT GAACAGATAA ATACGAAACT GCCATCATCA 
TTTGTAGAAA AACTGTTTAT ACCATCATCT AAACTACTAT TCTTGCGTTA TCATAAAGAA 
AAAGAGGTTG TTGCTGTAGC CCATGCTGTT TATCAAGCAG TGCTCAGCTT GAAGAATATT 
CCTGTTTTGG AGACTGCCTA TAAGTTAATA TTGGGAGAAA TGACTTGTGC CCTAAACAAC 
CTCCTACACA GTCTACAACT TCCTGAGGCC TGTTCTGAAA TAAAACATGA GGCTTTTAAG 
AATCATGTGT TCAATGTAGA CAATGCAAAA TTTGTAGTTA TATTTGACCT CAGTGCCCTG 
ACTACAATTG GAAATGCCAA AAACTCACTA ATAGGGATGT GGGCGCTATC TCCAACTGTC 
TTTGCACTTC TGAGTAAGAA TCTGATGATT GTGCACAGTG ACCTGGCTGT TCACTTCCCT 
GCCATTCAGT ATGCTGTGCT CTACACATTG TATTCTCATT GTACCAGGCA TGATCACTTT 
ATCTCTAGTA GCCTCAGTTC TTCCTCTCCT TCTTTGTTTG ATGGAGCTGT GATTAGCACT 
GTAACTACGG CTACAAAGAA ACATTTCTCA ATTATATTAA ATCTTCTGGG AATATTACTT 
AAGAAAGATA ACCTTAACCA GGACACGAGG AAACTGTTAA TGACTTGGGC TTTGGAAGCA 
GCTGTTTTAA TGAAGAAGTC TGAAACATAC GCACCTTTAT TCTCTCTTCC GTCTTTCCAT 
AAATTTTGCA AAGGCCTTTT AGCCAACACT CTCGTTGAAG ATGTGAATAT CTGTCTGCAG 
GCATGCAGCA GTCTACATGC TCTGTCCTCT TCCTTGCCAG ATGATCTTTT ACAGAGATGT 
GTCGATGTTT GCCGTGTTCA ACTAGTGCAC AGTGGAACTC GTATTCGACA AGCATTTGGA 
AAACTGTTGA AATCAATTCC TTTAGATGTT GTCCTAAGCA ATAACAATCA CACAGAAATT 
CAAGAAATTT CTTTAGCATT AAGAAGTCAC ATGAGTAAAG CACCAAGTAA TACATTCCAC 
CCCCAAGATT TCTCTGATGT TATTAGTTTT ATTTTGTATG GGAACTCTCA TAGAACAGGG 
AAGGACAATT GGTTGGAAAG ACTGTTCTAT AGCTGCCAGA GACTGGATAA GCGTGACCAG 
TCAACAATTC CACGCAATCT CCTGAAGACA GATGCTGTCC TTTGGCAGTG GGCCATATGG 
GAAGCTGCAC AATTCACTGT TCTTTCTAAG CTGAGAACCC CACTGGGCAG AGCTCAAGAC 
ACCTTCCAGA CAATTGAAGG TATCATTCGA AGTCTCGCAG CTCACACATT AAACCCTGAT 
CAGGATGTTA GTCAGTGGAC AACTGCAGAC AATGATGAAG GCCATGGTAA CAACCAACTT 
AGACTTGTTC TTCTTCTGCA GTATCTGGAA AATCTGGAGA AATTAATGTA TAATGCATAC 
GAGGGATGTG CTAATGCATT AACTTCACCT CCCAAGGTCA TTAGAACTTT TTTCTATACC 
AATCGCCAAA CTTGTCAGGA CTGGCTAACG CGGATTCGAC TCTCCATCAT GAGGGTAGGA 
TTGTTGGCAG GCCAGCCTGC AGTGACAGTG AGACATGGCT TTGACTTGCT TACAGAGATG 
AAAACAACCA GCCTATCTCA GGGGAATGAA TTGGAAGTAA CCATTATGAT GGTGGTAGAA 
GCATTATGTG AACTTCATTG TCCTGAAGCT ATACAGGGAA TTGCTGTCTG GTCATCATCT 
ATTGTTGGAA AAAATCTTCT GTGGATTAAC TCAGTGGCTC AACAGGCTGA AGGGAGGTTT 
GAAAAGGCCT CTGTGGAGTA CCAGGAACAC CTGTGTGCCA TGACAGGTGT TGATTGCTGC 
ATCTCCAGCT TTGACAAATC GGTGCTCACC TTAGCCAATG CTGGGCGTAA CAGTGCCAGC 
CCGAAACATT CTCTGAATGG TGAATCCAGA AAAACTGTGC TGTCCAAACC GACTGACTCT 
TCCCCTGAGG TTATAAATTA TTTAGGAAAT AAAGCATGTG AGTGCTACAT CTCAATTGCC 
GATTGGGCTG CTGTGCAGGA ATGGCAGAAC GCTATCCATG ACTTGAAAAA GAGTACCAGT 
AGCACTTCCC TCAACCTGAA AGCTGACTTC AACTATATAA AATCATTAAG CAGCTTTGAG 
TCTGGAAAAT TTGTTGAATG TACCGAGCAG TTAGAATTGT TACCAGGAGA AAATATCAAT 
CTACTTGCTG GAGGATCAAA AGAAAAAATA GACATGAAAA AACTGCTTCC TAACATGTTA 
AGTCCGGATC CGAGGGAACT TCAGAAATCC ATTGAAGTTC AATTGTTAAG AAGTTCTGTT 
TGTTTGGCAA CTGCTTTAAA CCCGATAGAA CAAGATCAGA AGTGGCAGTC TATAACTGAA 
AATGTGGTAA AGTACTTGAA GCAAACATCC CGCATCGCTA TTGGACCTCT GAGACTTTCT 
ACTTTAACAG TTTCACAGTC TTTGCCAGTT CTAAGTACCT TGCAGCTGTA TTGCTCATCT 
GCTTTGGAGA ACACAGTTTC TAACAGACTT TCAACAGAGG ACTGTCTTAT TCCACTCTTC 
AGTGAAGCTT TACGTTCATG TAAACAGCAT GACGTGAGGC CATGGATGCA GGCATTAAGG 
TATACTATGT ACCAGAATCA GTTGTTGGAG AAAATTAAAG AACAAACAGT CCCAATTAGA 
AGCCATCTCA TGGAATTAGG TCTAACAGCA GCAAAATTTG CTAGAAAACG AGGGAATGTG 
TCCCTTGCAA CAAGACTGCT GGCACAGTGC AGTGAAGTTC AGCTGGGAAA GACCACCACT 
GCACAGGATT TAGTCCAACA TTTTAAAAAA CTATCAACCC AAGGTCAAGT GGATGAAAAA 
TGGGGGCCCG AACTTGATAT TGAAAAAACC AAATTGCTTT ATACAGCAGG CCAGTCAACA 
CATGCAATGG AAATGTTGAG TTCTTGTGCC ATATCTTTCT GCAAGTCTGT GAAAGCTGAA 
TATGCAGTTG CTAAATCAAT TCTGACACTG GCTAAATGGA TCCAGGCAGA ATGGAAAGAG 
ATTTCAGGAC AGCTGAAACA GGTTTACAGA GCTCAGCACC AACAGAACTT CACAGGTCTT 
TCTACTTTGT CTAAAAACAT ACTCACTCTA ATAGAACTGC CATCTGTTAA TACGATGGAA 
GAAGAGTATC CTCGGATCGA GAGTGAATCT ACAGTGCATA TTGGAGTTGG AGAACCTGAC 
TTCATTTTGG GACAGTTGTA TCACCTGTCT TCAGTACAGG CACCTGAAGT AGCCAAATCT 
TGGGCAGCGT TGGCCAGCTG GGCTTATAGG TGGGGCAGAA AGGTGGTTGA CAATGCCAGT 
CAGGGAGAAG GTGTTCGTCT GCTGCCTAGA GAAAAATCTG AAGTTCAGAA TCTACTTCCA 
GACACTATAA CTGAGGAAGA GAAAGAGAGA ATATATGGTA TTCTTGGACA GGCTGTGTGT 
CGGCCGGCGG GGATTCAGGA TGAAGATATA ACACTTCAGA TAACTGAGAG TGAAGACAAC 
GAAGAAGATG ACATGGTTGA TGTTATCTGG CGTCAGTTGA TATCAAGCTG CCCATGGCTT 
TCAGAACTTG ATGAAAGTGC AACTGAAGGA GTTATTAAAG TGTGGAGGAA AGTTGTAGAT 
AGAATATTCA GCCTGTACAA ACTCTCTTGC AGTGCATACT TTACTTTCCT TAAACTCAAC 
GCTGGTCAAA TTCCTTTAGA TGAGGATGAC CCTAGGCTGC ATTTAAGTCA CAGAGTGGAA 
CAGAGCACTG ATGACATGAT TGTGATGGCC ACATTGCGCC TGCTGCGGTT GCTCGTGAAG 
CATGCTGGTG AGCTTCGGCA GTATCTGGAG CACGGCTTGG AGACAACACC CACTGCACCA 
TGGAGAGGAA TTATTCCGCA ACTTTTCTCA CGCTTAAACC ACCCTGAAGT GTATGTGCGC 
CAAAGTATTT GTAACCTTCT CTGCCGTGTG GCTCAAGATT CCCCACATCT CATATTGTAT 
CCTGCAATAG TGGGTACCAT ATCGCTTAGT AGTGAATCCC AGGCTTCAGG AAATAAATTT 
TCCACTGCAA TTCCAACTTT ACTTGGCAAT ATTCAAGGAG AAGAATTGCT GGTTTCTGAA 
TGTGAGGGAG GAAGTCCTCC TGCATCTCAG GATAGCAATA AGGATGAACC TAAAAGTGGA 
TTAAATGAAG ACCAAGCCAT GATGCAGGAT TGTTACAGCA AAATTGTAGA TAAGCTGTCC 
TCTGCAAACC CCACCATGGT ATTACAGGTT CAGATGCTCG TGGCTGAACT GCGCAGGGTC 
ACTGTGCTCT GGGATGAGCT CTGGCTGGGA GTTTTGCTGC AACAACACAT GTATGTCCTG 
AGACGAATTC AGCAGCTTGA AGATGAGGTG AAGAGAGTCC AGAACAACAA CACCTTACGC 
AAAGAAGAGA AAATTGCAAT CATGAGGGAG AAGCACACAG CTTTGATGAA GCCCATCGTA 
TTTGCTTTGG AGCATGTGAG GAGTATCACA GCGGCTCCTG CAGAAACACC TCATGAAAAA 
TGGTTTCAGG ATAACTATGG TGATGCCATT GAAAATGCCC TAGAAAAACT GAAGACTCCA 
TTGAACCCTG CAAAGCCTGG GAGCAGCTGG ATTCCATTTA AAGAGATAAT GCTAAGTTTG 
CAACAGAGAG CACAGAAACG TGCAAGTTAC ATCTTGCGTC TTGAAGAAAT CAGTCCATGG 
TTGGCTGCCA TGACTAACAC TGAAATTGCT CTTCCTGGGG AAGTCTCAGC CAGAGACACT 
GTCACAATCC ATAGTGTGGG CGGAACCATC ACAATCTTAC CGACTAAAAC CAAGCCAAAG 
AAACTTCTCT TTCTTGGATC AGATGGGAAG AGCTATCCTT ATCTTTTCAA AGGACTGGAG 
GATTTACATC TGGATGAGAG AATAATGCAG TTCCTATCTA TTGTGAATAC CATGTTTGCT 
ACAATTAATC GCCAAGAAAC ACCCCGGTTC CATGCTCGAC ACTATTCTGT AACACCACTA 
GGAACAAGAT CAGGACTAAT CCAGTGGGTA GATGGAGCCA CACCCTTATT TGGTCTTTAC 
AAACGATGGC AACAACGGGA AGCTGCCTTA CAAGCACAAA AGGCCCAAGA TTCCTACCAA 
ACTCCTCAGA ATCCTGGAAT TGTACCCCGT CCTAGTGAAC TTTATTACAG TAAAATTGGC 
CCTGCTTTGA AAACAGTTGG GCTTAGCCTG GATGTGTCCC GTCGGGATTG GCCTCTTCAT 
GTAATGAAGG CAGTATTGGA AGAGTTAATG GAGGCCACAC CCCCGAATCT CCTTGCCAAA 
GAGCTCTGGT CATCTTGCAC AACACCTGAT GAATGGTGGA GAGTTACGCA GTCTTATGCA 
AGATCTACTG CAGTCATGTC TATGGTTGGA TACATAATTG GCCTTGGAGA CAGACATCTG 
GATAATGTTC TTATAGATAT GACGACTGGA GAAGTTGTTC ACATAGATTA CAATGTTTGC 
TTTGAAAAAG GTAATATAAA AGTAGACTTT ACATTTATGA GGGCCAAATG TTTTTTGGTG 
AAAGGGCAAA AATATAATGA TTTGTCTTTT CAAGGTAAAA GCCTTAGAGT TCCTGAGAAA 
GTACCTTTTC GAATGACACA AAACATTGAA ACAGCACTGG GTGTAACTGG AGTAGAAGGT 
GTATTTAGGC TTTCATGTGA GCAGGTTTTA CACATTATGC GGCGTGGCAG AGAGACCCTG 
CTGACGCTGC TGGAGGCCTT TGTGTACGAC CCTCTGGTGG ACTGGACAGC AGGAGGCGAG 
GCTGGGTTTG CTGGTGCTGT CTATGGTGGA GGTGGCCAGC AGGCCGAGAG CAAGCAGAGC 
AAGAGAGAGA TGGAGCGAGA GATCACCCGC AGCCTGTTTT CTTCTAGAGT AGCTGAGATT 
AAGGTGAACT GGTTTAAGAA TAGAGATGAG ATGCTGGTTG TGCTTCCCAA GTTGGACGGT 
AGCTTAGATG AATACCTAAG CTTGCAAGAG CAACTGACAG ATGTGGAAAA ACTGCAGGGC 
AAACTACTGG AGGAAATAGA GTTTCTAGAA GGAGCTGAAG GGGTGGATCA TCCTTCTCAT 
ACTCTGCAAC ACAGGTATTC TGAGCACACC CAACTACAGA CTCAGCAAAG AGCTGTTCAG 
GAAGCAATCC AGGTGAAGCT GAATGAATTT GAACAATGGA TAACACATTA TCAGGCTGCA 
TTCAATAATT TAGAAGCAAC ACAGCTTGCA AGCTTGCTTC AAGAGATAAG CACACAAATG 
GACCTTGGTC CTCCAAGTTA CGTGCCAGCA ACAGCCTTTC TGCAGAATGC TGGTCAGGCC 
CACTTGATTA GCCAGTGCGA GCAGCTGGAG GGGGAGGTTG GTGCTCTCCT GCAGCAGAGG 
CGCTCCGTGC TCCGTGGCTG TCTGGAGCAA CTGCATCACT ATGCAACCGT GGCCCTGCAG 
TATCCGAAGG CCATATTTCA GAAACATCGA ATTGAACAGT GGAAGACCTG GATGGAAGAG 
CTCATCTGTA ACACCACAGT AGAGCGTTGT CAAGAGCTCT ATAGGAAATA TGAAATGCAA 
TATGCTCCCC AGCCACCCCC AACAGTGTGT CAGTTCATCA CTGCCACTGA AATGACCCTG 
CAGCGATACG CAGCAGACAT CAACAGCAGA CTTATTAGAC AAGTGGAACG CTTGAAACAG 
GAAGCTGTCA CTGTGCCAGT TTGTGAAGAT CAGTTGAAAG AAATTGAACG TTGCATTAAA 
GTTTTCCTTC ATGAGAATGG AGAAGAAGGA TCTTTGAGTC TAGCAAGTGT TATTATTTCT 
GCCCTTTGTA CCCTTACAAG GCGTAACCTG ATGATGGAAG GTGCAGCGTC AAGTGCTGGA 
GAACAGCTGG TTGATCTGAC TTCTCGGGAT GGAGCCTGGT TCTTGGAGGA ACTCTGCAGT 
ATGAGCGGAA ACGTCACCTG CTTGGTTCAG TTACTGAAGC AGTGCCACCT GGTGCCACAG 
GACTTAGATA TCCCGAACCC CATGGAAGCG TCTGAGACAG TTCACTTAGC CAATGGAGTG 
TATACCTCAC TTCAGGAATT GAATTCGAAT TTCCGGCAAA TCATATTTCC AGAAGCACTT 
CGATGTTTAA TGAAAGGGGA ATACACGTTA GAAAGTATGC TGCATGAACT GGACGGTCTT 
ATTGAGCAGA CCACCGATGG CGTTCCCCTG CAGACTCTAG TGGAATCTCT TCAGGCCTAC 
TTAAGAAACG CAGCTATGGG ACTGGAAGAA GAAACACATG CTCATTACAT CGATGTTGCC 
AGACTACTAC ATGCTCAGTA CGGTGAATTA ATCCAACCGA GAAATGGTTC AGTTGATGAA 
ACACCCAAAA TGTCAGCTGG CCAGATGCTT TTGGTAGCAT TCGATGGCAT GTTTGCTCAA 
GTTGAAACTG CTTTCAGCTT ATTAGTTGAA AAGTTGAACA AGATGGAAAT TCCCATAGCT 
TGGCGAAAGA TTGACATCAT AAGGGAAGCC AGGAGTACTC AAGTTAATTT TTTTGATGAT 
GATAATCACC GGCAGGTGCT AGAAGAGATT TTCTTTCTAA AAAGACTACA GACTATTAAG 
GAGTTCTTCA GGCTCTGTGG TACCTTTTCT AAAACATTGT CAGGATCAAG TTCACTTGAA 
GATCAGAATA CTGTGAATGG GCCTGTACAG ATTGTCAATG TGAAAACCCT TTTTAGAAAC 
TCTTGTTTCA GTGAAGACCA AATGGCCAAA CCTATCAAGG CATTCACAGC TGACTTTGTG 
AGGCAGCTCT TGATAGGGCT ACCCAACCAA GCCCTCGGAC TCACACTGTG CAGTTTTATC 
AGTGCTCTGG GTGTAGACAT CATTGCTCAA GTAGAGGCAA AGGACTTTGG TGCCGAAAGC 
AAAGTTTCTG TTGATGATCT CTGTAAGAAA GCGGTGGAAC ATAACATCCA GATAGGGAAG 
TTCTCTCAGC TGGTTATGAA CAGGGCAACT GTGTTAGCAA GTTCTTACGA CACTGCCTGG 
AAGAAGCATG ACTTGGTGCG AAGGCTAGAA ACCAGTATTT CTTCTTGTAA GACAAGCCTG 
CAGCGGGTTC AGCTGCATAT TGCCATGTTT CAGTGGCAAC ATGAAGATCT ACTTATCAAT 
AGACCACAAG CCATGTCAGT CACACCTCCC CCACGGTCTG CTATCCTAAC CAGCATGAAA 
AAGAAGCTGC ATACCCTGAG CCAGATTGAA ACTTCTATTG CAACAGTTCA GGAGAAGCTA 
GCTGCACTTG AATCAAGTAT TGAACAGCGA CTCAAGTGGG CAGGTGGTGC CAACCCTGCA 
TTGGCCCCTG TACTACAAGA TTTTGAAGCA ACGATAGCTG AAAGAAGAAA TCTTGTCCTT 
AAAGAGAGCC AAAGAGCAAG TCAGGTCACA TTTCTCTGCA GCAATATCAT TCATTTTGAA 
AGTTTACGAA CAAGAACTGC AGAAGCCTTA AACCTGGATG CGGCGTTATT TGAACTAATC 
AAGCGATGTC AGCAGATGTG TTCGTTTGCA TCACAGTTTA ACAGTTCAGT GTCTGAGTTA 
GAGCTTCGTT TATTACAGAG AGTGGACACT GGTCTTGAAC ATCCTATTGG CAGCTCTGAA 
TGGCTTTTGT CAGCACACAA ACAGTTGACC CAGGATATGT CTACTCAGAG GGCAATTCAG 
ACAGAGAAAG AGCAGCAGAT AGAAACGGTC TGTGAAACAA TTCAGAATCT GGTTGATAAT 
ATAAAGACTG TGCTCACTGG TCATAACCGA CAGCTTGGAG ATGTCAAACA TCTCTTGAAA 
GCTATGGCTA AGGATGAAGA AGCTGCTCTG GCAGATGGTG AAGATGTTCC CTATGAGAAC 
AGTGTTAGGC AGTTTTTGGG TGAATATAAA TCATGGCAAG ACAACATTCA AACAGTTCTA 
TTTACATTAG TCCAGGCTAT GGGTCAGGTT CGAAGTCAAG AACACGTTGA AATGCTCCAG 
GAAATCACTC CCACCTTGAA AGAACTGAAA ACACAAAGTC AGAGTATCTA TAATAATTTA 
GTGAGTTTTG CATCACCCTT AGTCACCGAT GCAACAAATG AATGTTCGAG TCCAACGTCA 
TCTGCTACTT ATCAGCCATC CTTCGCTGCA GCAGTCCGGA GTAACACTGG CCAGAAGACT 
CAGCCTGATG TCATGTCACA GAATGCTAGA AAGCTGATCC AAAAAAATCT TGCTACATCA 
GCTGATACTC CACCAAGCAC CGTTCCAGGA ACTGGCAAGA GTGTTGCTTG TAGTCCTAAA 
AAGGCAGTCA GAGACCCTAA AACTGGGAAA GCGGTGCAAG AGAGAAACTC CTATGCAGTG 
AGTGTGTGGA AGAGAGTGAA AGCCAAGTTA GAGGGCCGAG ATGTTGATCC GAATAGGAGG 
ATGTCAGTTG CTGAACAGGT TGACTATGTC ATTAAGGAAG CAACTAATCT AGATAACTTG 
GCTCAGCTGT ATGAAGGTTG GACAGCCTGG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_024450198.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305966.1 Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X6, mRNA.

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CloneID	OHu104104
Clone ID Related Accession (Same CDS sequence)	XM_024450199.1 , XM_024450198.1
Accession Version	XM_024450198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATAATTTAAA ATATTCTTCA 
TCCAGAGATA GAGGTGGTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACGCTGACA TACAGAATGA CGAAAAGGAG 
AGATCGATGT CTTATTCTGA TGAGTCTCGA CTGTCGAATC TTCTTCGGAG GATCACCCGG 
GAAGACGACA GAGACCGAAG ATTGGCTACT GTAAAGCAGT TGAAAGAATT TATTCAGCAA 
CCAGAAAATA AGCTGGTACT AGTTAAACAA TTGGATAATA TCTTGGCTGC TGTACATGAC 
GTGCTTAATG AAAGTAGCAA ATTGCTTCAG GAGTTGAGAC AGGAGGGAGC TTGCTGTCTT 
GGCCTTCTTT GTGCTTCTCT GAGCTATGAG GCTGAGAAGA TCTTCAAGTG GATTTTTAGC 
AAATTTAGCT CATCTGCAAA AGATGAAGTT AAACTCCTCT ACTTATGTGC CACCTACAAA 
GCACTAGAGA CTGTAGGAGA AAAGAAAGCC TTTTCATCTG TAATGCAGCT TGTAATGACC 
AGCCTGCAGT CTATTCTTGA AAATGTGGAT ACACCAGAAT TGCTTTGTAA ATGTGTTAAG 
TGCATTCTTT TGGTGGCTCG ATGTTACCCT CATATTTTCA GCACTAATTT TAGGGATACA 
GTTGATATAT TAGTTGGATG GCATATAGAT CATACTCAGA AACCTTCGCT CACGCAGCAG 
GTATCTGGGT GGTTGCAGAG TTTGGAGCCA TTTTGGGTAG CTGATCTTGC ATTTTCTACT 
ACTCTTCTTG GTCAGTTTCT GGAAGACATG GAAGCATATG CTGAGGACCT CAGCCATGTG 
GCCTCTGGGG AATCAGTGGA TGAAGATGTC CCTCCTCCAT CAGTGTCATT ACCAAAGCTG 
GCTGCACTTC TCCGGGTATT TAGTACTGTG GTGAGGAGCA TTGGGGAACG CTTCAGCCCA 
ATTCGGGGTC CTCCAATTAC TGAGGCATAT GTAACAGATG TTCTGTACAG AGTAATGAGA 
TGTGTGACGG CTGCAAACCA GGTGTTTTTT TCTGAGGCTG TGTTGACAGC TGCTAATGAG 
TGTGTTGGTG TTTTGCTCGG CAGCTTGGAT CCTAGCATGA CTATACATTG TGACATGGTC 
ATTACATATG GATTAGACCA ACTGGAGAAT TGCCAGACTT GTGGTACCGA TTATATCATC 
TCAGTCTTGA ATTTACTCAC GCTGATTGTT GAACAGATAA ATACGAAACT GCCATCATCA 
TTTGTAGAAA AACTGTTTAT ACCATCATCT AAACTACTAT TCTTGCGTTA TCATAAAGAA 
AAAGAGGTTG TTGCTGTAGC CCATGCTGTT TATCAAGCAG TGCTCAGCTT GAAGAATATT 
CCTGTTTTGG AGACTGCCTA TAAGTTAATA TTGGGAGAAA TGACTTGTGC CCTAAACAAC 
CTCCTACACA GTCTACAACT TCCTGAGGCC TGTTCTGAAA TAAAACATGA GGCTTTTAAG 
AATCATGTGT TCAATGTAGA CAATGCAAAA TTTGTAGTTA TATTTGACCT CAGTGCCCTG 
ACTACAATTG GAAATGCCAA AAACTCACTA ATAGGGATGT GGGCGCTATC TCCAACTGTC 
TTTGCACTTC TGAGTAAGAA TCTGATGATT GTGCACAGTG ACCTGGCTGT TCACTTCCCT 
GCCATTCAGT ATGCTGTGCT CTACACATTG TATTCTCATT GTACCAGGCA TGATCACTTT 
ATCTCTAGTA GCCTCAGTTC TTCCTCTCCT TCTTTGTTTG ATGGAGCTGT GATTAGCACT 
GTAACTACGG CTACAAAGAA ACATTTCTCA ATTATATTAA ATCTTCTGGG AATATTACTT 
AAGAAAGATA ACCTTAACCA GGACACGAGG AAACTGTTAA TGACTTGGGC TTTGGAAGCA 
GCTGTTTTAA TGAAGAAGTC TGAAACATAC GCACCTTTAT TCTCTCTTCC GTCTTTCCAT 
AAATTTTGCA AAGGCCTTTT AGCCAACACT CTCGTTGAAG ATGTGAATAT CTGTCTGCAG 
GCATGCAGCA GTCTACATGC TCTGTCCTCT TCCTTGCCAG ATGATCTTTT ACAGAGATGT 
GTCGATGTTT GCCGTGTTCA ACTAGTGCAC AGTGGAACTC GTATTCGACA AGCATTTGGA 
AAACTGTTGA AATCAATTCC TTTAGATGTT GTCCTAAGCA ATAACAATCA CACAGAAATT 
CAAGAAATTT CTTTAGCATT AAGAAGTCAC ATGAGTAAAG CACCAAGTAA TACATTCCAC 
CCCCAAGATT TCTCTGATGT TATTAGTTTT ATTTTGTATG GGAACTCTCA TAGAACAGGG 
AAGGACAATT GGTTGGAAAG ACTGTTCTAT AGCTGCCAGA GACTGGATAA GCGTGACCAG 
TCAACAATTC CACGCAATCT CCTGAAGACA GATGCTGTCC TTTGGCAGTG GGCCATATGG 
GAAGCTGCAC AATTCACTGT TCTTTCTAAG CTGAGAACCC CACTGGGCAG AGCTCAAGAC 
ACCTTCCAGA CAATTGAAGG TATCATTCGA AGTCTCGCAG CTCACACATT AAACCCTGAT 
CAGGATGTTA GTCAGTGGAC AACTGCAGAC AATGATGAAG GCCATGGTAA CAACCAACTT 
AGACTTGTTC TTCTTCTGCA GTATCTGGAA AATCTGGAGA AATTAATGTA TAATGCATAC 
GAGGGATGTG CTAATGCATT AACTTCACCT CCCAAGGTCA TTAGAACTTT TTTCTATACC 
AATCGCCAAA CTTGTCAGGA CTGGCTAACG CGGATTCGAC TCTCCATCAT GAGGGTAGGA 
TTGTTGGCAG GCCAGCCTGC AGTGACAGTG AGACATGGCT TTGACTTGCT TACAGAGATG 
AAAACAACCA GCCTATCTCA GGGGAATGAA TTGGAAGTAA CCATTATGAT GGTGGTAGAA 
GCATTATGTG AACTTCATTG TCCTGAAGCT ATACAGGGAA TTGCTGTCTG GTCATCATCT 
ATTGTTGGAA AAAATCTTCT GTGGATTAAC TCAGTGGCTC AACAGGCTGA AGGGAGGTTT 
GAAAAGGCCT CTGTGGAGTA CCAGGAACAC CTGTGTGCCA TGACAGGTGT TGATTGCTGC 
ATCTCCAGCT TTGACAAATC GGTGCTCACC TTAGCCAATG CTGGGCGTAA CAGTGCCAGC 
CCGAAACATT CTCTGAATGG TGAATCCAGA AAAACTGTGC TGTCCAAACC GACTGACTCT 
TCCCCTGAGG TTATAAATTA TTTAGGAAAT AAAGCATGTG AGTGCTACAT CTCAATTGCC 
GATTGGGCTG CTGTGCAGGA ATGGCAGAAC GCTATCCATG ACTTGAAAAA GAGTACCAGT 
AGCACTTCCC TCAACCTGAA AGCTGACTTC AACTATATAA AATCATTAAG CAGCTTTGAG 
TCTGGAAAAT TTGTTGAATG TACCGAGCAG TTAGAATTGT TACCAGGAGA AAATATCAAT 
CTACTTGCTG GAGGATCAAA AGAAAAAATA GACATGAAAA AACTGCTTCC TAACATGTTA 
AGTCCGGATC CGAGGGAACT TCAGAAATCC ATTGAAGTTC AATTGTTAAG AAGTTCTGTT 
TGTTTGGCAA CTGCTTTAAA CCCGATAGAA CAAGATCAGA AGTGGCAGTC TATAACTGAA 
AATGTGGTAA AGTACTTGAA GCAAACATCC CGCATCGCTA TTGGACCTCT GAGACTTTCT 
ACTTTAACAG TTTCACAGTC TTTGCCAGTT CTAAGTACCT TGCAGCTGTA TTGCTCATCT 
GCTTTGGAGA ACACAGTTTC TAACAGACTT TCAACAGAGG ACTGTCTTAT TCCACTCTTC 
AGTGAAGCTT TACGTTCATG TAAACAGCAT GACGTGAGGC CATGGATGCA GGCATTAAGG 
TATACTATGT ACCAGAATCA GTTGTTGGAG AAAATTAAAG AACAAACAGT CCCAATTAGA 
AGCCATCTCA TGGAATTAGG TCTAACAGCA GCAAAATTTG CTAGAAAACG AGGGAATGTG 
TCCCTTGCAA CAAGACTGCT GGCACAGTGC AGTGAAGTTC AGCTGGGAAA GACCACCACT 
GCACAGGATT TAGTCCAACA TTTTAAAAAA CTATCAACCC AAGGTCAAGT GGATGAAAAA 
TGGGGGCCCG AACTTGATAT TGAAAAAACC AAATTGCTTT ATACAGCAGG CCAGTCAACA 
CATGCAATGG AAATGTTGAG TTCTTGTGCC ATATCTTTCT GCAAGTCTGT GAAAGCTGAA 
TATGCAGTTG CTAAATCAAT TCTGACACTG GCTAAATGGA TCCAGGCAGA ATGGAAAGAG 
ATTTCAGGAC AGCTGAAACA GGTTTACAGA GCTCAGCACC AACAGAACTT CACAGGTCTT 
TCTACTTTGT CTAAAAACAT ACTCACTCTA ATAGAACTGC CATCTGTTAA TACGATGGAA 
GAAGAGTATC CTCGGATCGA GAGTGAATCT ACAGTGCATA TTGGAGTTGG AGAACCTGAC 
TTCATTTTGG GACAGTTGTA TCACCTGTCT TCAGTACAGG CACCTGAAGT AGCCAAATCT 
TGGGCAGCGT TGGCCAGCTG GGCTTATAGG TGGGGCAGAA AGGTGGTTGA CAATGCCAGT 
CAGGGAGAAG GTGTTCGTCT GCTGCCTAGA GAAAAATCTG AAGTTCAGAA TCTACTTCCA 
GACACTATAA CTGAGGAAGA GAAAGAGAGA ATATATGGTA TTCTTGGACA GGCTGTGTGT 
CGGCCGGCGG GGATTCAGGA TGAAGATATA ACACTTCAGA TAACTGAGAG TGAAGACAAC 
GAAGAAGATG ACATGGTTGA TGTTATCTGG CGTCAGTTGA TATCAAGCTG CCCATGGCTT 
TCAGAACTTG ATGAAAGTGC AACTGAAGGA GTTATTAAAG TGTGGAGGAA AGTTGTAGAT 
AGAATATTCA GCCTGTACAA ACTCTCTTGC AGTGCATACT TTACTTTCCT TAAACTCAAC 
GCTGGTCAAA TTCCTTTAGA TGAGGATGAC CCTAGGCTGC ATTTAAGTCA CAGAGTGGAA 
CAGAGCACTG ATGACATGAT TGTGATGGCC ACATTGCGCC TGCTGCGGTT GCTCGTGAAG 
CATGCTGGTG AGCTTCGGCA GTATCTGGAG CACGGCTTGG AGACAACACC CACTGCACCA 
TGGAGAGGAA TTATTCCGCA ACTTTTCTCA CGCTTAAACC ACCCTGAAGT GTATGTGCGC 
CAAAGTATTT GTAACCTTCT CTGCCGTGTG GCTCAAGATT CCCCACATCT CATATTGTAT 
CCTGCAATAG TGGGTACCAT ATCGCTTAGT AGTGAATCCC AGGCTTCAGG AAATAAATTT 
TCCACTGCAA TTCCAACTTT ACTTGGCAAT ATTCAAGGAG AAGAATTGCT GGTTTCTGAA 
TGTGAGGGAG GAAGTCCTCC TGCATCTCAG GATAGCAATA AGGATGAACC TAAAAGTGGA 
TTAAATGAAG ACCAAGCCAT GATGCAGGAT TGTTACAGCA AAATTGTAGA TAAGCTGTCC 
TCTGCAAACC CCACCATGGT ATTACAGGTT CAGATGCTCG TGGCTGAACT GCGCAGGGTC 
ACTGTGCTCT GGGATGAGCT CTGGCTGGGA GTTTTGCTGC AACAACACAT GTATGTCCTG 
AGACGAATTC AGCAGCTTGA AGATGAGGTG AAGAGAGTCC AGAACAACAA CACCTTACGC 
AAAGAAGAGA AAATTGCAAT CATGAGGGAG AAGCACACAG CTTTGATGAA GCCCATCGTA 
TTTGCTTTGG AGCATGTGAG GAGTATCACA GCGGCTCCTG CAGAAACACC TCATGAAAAA 
TGGTTTCAGG ATAACTATGG TGATGCCATT GAAAATGCCC TAGAAAAACT GAAGACTCCA 
TTGAACCCTG CAAAGCCTGG GAGCAGCTGG ATTCCATTTA AAGAGATAAT GCTAAGTTTG 
CAACAGAGAG CACAGAAACG TGCAAGTTAC ATCTTGCGTC TTGAAGAAAT CAGTCCATGG 
TTGGCTGCCA TGACTAACAC TGAAATTGCT CTTCCTGGGG AAGTCTCAGC CAGAGACACT 
GTCACAATCC ATAGTGTGGG CGGAACCATC ACAATCTTAC CGACTAAAAC CAAGCCAAAG 
AAACTTCTCT TTCTTGGATC AGATGGGAAG AGCTATCCTT ATCTTTTCAA AGGACTGGAG 
GATTTACATC TGGATGAGAG AATAATGCAG TTCCTATCTA TTGTGAATAC CATGTTTGCT 
ACAATTAATC GCCAAGAAAC ACCCCGGTTC CATGCTCGAC ACTATTCTGT AACACCACTA 
GGAACAAGAT CAGGACTAAT CCAGTGGGTA GATGGAGCCA CACCCTTATT TGGTCTTTAC 
AAACGATGGC AACAACGGGA AGCTGCCTTA CAAGCACAAA AGGCCCAAGA TTCCTACCAA 
ACTCCTCAGA ATCCTGGAAT TGTACCCCGT CCTAGTGAAC TTTATTACAG TAAAATTGGC 
CCTGCTTTGA AAACAGTTGG GCTTAGCCTG GATGTGTCCC GTCGGGATTG GCCTCTTCAT 
GTAATGAAGG CAGTATTGGA AGAGTTAATG GAGGCCACAC CCCCGAATCT CCTTGCCAAA 
GAGCTCTGGT CATCTTGCAC AACACCTGAT GAATGGTGGA GAGTTACGCA GTCTTATGCA 
AGATCTACTG CAGTCATGTC TATGGTTGGA TACATAATTG GCCTTGGAGA CAGACATCTG 
GATAATGTTC TTATAGATAT GACGACTGGA GAAGTTGTTC ACATAGATTA CAATGTTTGC 
TTTGAAAAAG GTAATATAAA AGTAGACTTT ACATTTATGA GGGCCAAATG TTTTTTGGTG 
AAAGGGCAAA AATATAATGA TTTGTCTTTT CAAGGTAAAA GCCTTAGAGT TCCTGAGAAA 
GTACCTTTTC GAATGACACA AAACATTGAA ACAGCACTGG GTGTAACTGG AGTAGAAGGT 
GTATTTAGGC TTTCATGTGA GCAGGTTTTA CACATTATGC GGCGTGGCAG AGAGACCCTG 
CTGACGCTGC TGGAGGCCTT TGTGTACGAC CCTCTGGTGG ACTGGACAGC AGGAGGCGAG 
GCTGGGTTTG CTGGTGCTGT CTATGGTGGA GGTGGCCAGC AGGCCGAGAG CAAGCAGAGC 
AAGAGAGAGA TGGAGCGAGA GATCACCCGC AGCCTGTTTT CTTCTAGAGT AGCTGAGATT 
AAGGTGAACT GGTTTAAGAA TAGAGATGAG ATGCTGGTTG TGCTTCCCAA GTTGGACGGT 
AGCTTAGATG AATACCTAAG CTTGCAAGAG CAACTGACAG ATGTGGAAAA ACTGCAGGGC 
AAACTACTGG AGGAAATAGA GTTTCTAGAA GGAGCTGAAG GGGTGGATCA TCCTTCTCAT 
ACTCTGCAAC ACAGGTATTC TGAGCACACC CAACTACAGA CTCAGCAAAG AGCTGTTCAG 
GAAGCAATCC AGGTGAAGCT GAATGAATTT GAACAATGGA TAACACATTA TCAGGCTGCA 
TTCAATAATT TAGAAGCAAC ACAGCTTGCA AGCTTGCTTC AAGAGATAAG CACACAAATG 
GACCTTGGTC CTCCAAGTTA CGTGCCAGCA ACAGCCTTTC TGCAGAATGC TGGTCAGGCC 
CACTTGATTA GCCAGTGCGA GCAGCTGGAG GGGGAGGTTG GTGCTCTCCT GCAGCAGAGG 
CGCTCCGTGC TCCGTGGCTG TCTGGAGCAA CTGCATCACT ATGCAACCGT GGCCCTGCAG 
TATCCGAAGG CCATATTTCA GAAACATCGA ATTGAACAGT GGAAGACCTG GATGGAAGAG 
CTCATCTGTA ACACCACAGT AGAGCGTTGT CAAGAGCTCT ATAGGAAATA TGAAATGCAA 
TATGCTCCCC AGCCACCCCC AACAGTGTGT CAGTTCATCA CTGCCACTGA AATGACCCTG 
CAGCGATACG CAGCAGACAT CAACAGCAGA CTTATTAGAC AAGTGGAACG CTTGAAACAG 
GAAGCTGTCA CTGTGCCAGT TTGTGAAGAT CAGTTGAAAG AAATTGAACG TTGCATTAAA 
GTTTTCCTTC ATGAGAATGG AGAAGAAGGA TCTTTGAGTC TAGCAAGTGT TATTATTTCT 
GCCCTTTGTA CCCTTACAAG GCGTAACCTG ATGATGGAAG GTGCAGCGTC AAGTGCTGGA 
GAACAGCTGG TTGATCTGAC TTCTCGGGAT GGAGCCTGGT TCTTGGAGGA ACTCTGCAGT 
ATGAGCGGAA ACGTCACCTG CTTGGTTCAG TTACTGAAGC AGTGCCACCT GGTGCCACAG 
GACTTAGATA TCCCGAACCC CATGGAAGCG TCTGAGACAG TTCACTTAGC CAATGGAGTG 
TATACCTCAC TTCAGGAATT GAATTCGAAT TTCCGGCAAA TCATATTTCC AGAAGCACTT 
CGATGTTTAA TGAAAGGGGA ATACACGTTA GAAAGTATGC TGCATGAACT GGACGGTCTT 
ATTGAGCAGA CCACCGATGG CGTTCCCCTG CAGACTCTAG TGGAATCTCT TCAGGCCTAC 
TTAAGAAACG CAGCTATGGG ACTGGAAGAA GAAACACATG CTCATTACAT CGATGTTGCC 
AGACTACTAC ATGCTCAGTA CGGTGAATTA ATCCAACCGA GAAATGGTTC AGTTGATGAA 
ACACCCAAAA TGTCAGCTGG CCAGATGCTT TTGGTAGCAT TCGATGGCAT GTTTGCTCAA 
GTTGAAACTG CTTTCAGCTT ATTAGTTGAA AAGTTGAACA AGATGGAAAT TCCCATAGCT 
TGGCGAAAGA TTGACATCAT AAGGGAAGCC AGGAGTACTC AAGTTAATTT TTTTGATGAT 
GATAATCACC GGCAGGTGCT AGAAGAGATT TTCTTTCTAA AAAGACTACA GACTATTAAG 
GAGTTCTTCA GGCTCTGTGG TACCTTTTCT AAAACATTGT CAGGATCAAG TTCACTTGAA 
GATCAGAATA CTGTGAATGG GCCTGTACAG ATTGTCAATG TGAAAACCCT TTTTAGAAAC 
TCTTGTTTCA GTGAAGACCA AATGGCCAAA CCTATCAAGG CATTCACAGC TGACTTTGTG 
AGGCAGCTCT TGATAGGGCT ACCCAACCAA GCCCTCGGAC TCACACTGTG CAGTTTTATC 
AGTGCTCTGG GTGTAGACAT CATTGCTCAA GTAGAGGCAA AGGACTTTGG TGCCGAAAGC 
AAAGTTTCTG TTGATGATCT CTGTAAGAAA GCGGTGGAAC ATAACATCCA GATAGGGAAG 
TTCTCTCAGC TGGTTATGAA CAGGGCAACT GTGTTAGCAA GTTCTTACGA CACTGCCTGG 
AAGAAGCATG ACTTGGTGCG AAGGCTAGAA ACCAGTATTT CTTCTTGTAA GACAAGCCTG 
CAGCGGGTTC AGCTGCATAT TGCCATGTTT CAGTGGCAAC ATGAAGATCT ACTTATCAAT 
AGACCACAAG CCATGTCAGT CACACCTCCC CCACGGTCTG CTATCCTAAC CAGCATGAAA 
AAGAAGCTGC ATACCCTGAG CCAGATTGAA ACTTCTATTG CAACAGTTCA GGAGAAGCTA 
GCTGCACTTG AATCAAGTAT TGAACAGCGA CTCAAGTGGG CAGGTGGTGC CAACCCTGCA 
TTGGCCCCTG TACTACAAGA TTTTGAAGCA ACGATAGCTG AAAGAAGAAA TCTTGTCCTT 
AAAGAGAGCC AAAGAGCAAG TCAGGTCACA TTTCTCTGCA GCAATATCAT TCATTTTGAA 
AGTTTACGAA CAAGAACTGC AGAAGCCTTA AACCTGGATG CGGCGTTATT TGAACTAATC 
AAGCGATGTC AGCAGATGTG TTCGTTTGCA TCACAGTTTA ACAGTTCAGT GTCTGAGTTA 
GAGCTTCGTT TATTACAGAG AGTGGACACT GGTCTTGAAC ATCCTATTGG CAGCTCTGAA 
TGGCTTTTGT CAGCACACAA ACAGTTGACC CAGGATATGT CTACTCAGAG GGCAATTCAG 
ACAGAGAAAG AGCAGCAGAT AGAAACGGTC TGTGAAACAA TTCAGAATCT GGTTGATAAT 
ATAAAGACTG TGCTCACTGG TCATAACCGA CAGCTTGGAG ATGTCAAACA TCTCTTGAAA 
GCTATGGCTA AGGATGAAGA AGCTGCTCTG GCAGATGGTG AAGATGTTCC CTATGAGAAC 
AGTGTTAGGC AGTTTTTGGG TGAATATAAA TCATGGCAAG ACAACATTCA AACAGTTCTA 
TTTACATTAG TCCAGGCTAT GGGTCAGGTT CGAAGTCAAG AACACGTTGA AATGCTCCAG 
GAAATCACTC CCACCTTGAA AGAACTGAAA ACACAAAGTC AGAGTATCTA TAATAATTTA 
GTGAGTTTTG CATCACCCTT AGTCACCGAT GCAACAAATG AATGTTCGAG TCCAACGTCA 
TCTGCTACTT ATCAGCCATC CTTCGCTGCA GCAGTCCGGA GTAACACTGG CCAGAAGACT 
CAGCCTGATG TCATGTCACA GAATGCTAGA AAGCTGATCC AAAAAAATCT TGCTACATCA 
GCTGATACTC CACCAAGCAC CGTTCCAGGA ACTGGCAAGA GTGTTGCTTG TAGTCCTAAA 
AAGGCAGTCA GAGACCCTAA AACTGGGAAA GCGGTGCAAG AGAGAAACTC CTATGCAGTG 
AGTGTGTGGA AGAGAGTGAA AGCCAAGTTA GAGGGCCGAG ATGTTGATCC GAATAGGAGG 
ATGTCAGTTG CTGAACAGGT TGACTATGTC ATTAAGGAAG CAACTAATCT AGATAACTTG 
GCTCAGCTGT ATGAAGGTTG GACAGCCTGG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305966.1
CDS	514..11349
Translation	MRFLRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGNIKVDFTFMRAKCFLVKGQKYNDLSFQGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_024450198.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450198.1

ORF Insert Sequence:

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATAATTTAAA ATATTCTTCA 
TCCAGAGATA GAGGTGGTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACGCTGACA TACAGAATGA CGAAAAGGAG 
AGATCGATGT CTTATTCTGA TGAGTCTCGA CTGTCGAATC TTCTTCGGAG GATCACCCGG 
GAAGACGACA GAGACCGAAG ATTGGCTACT GTAAAGCAGT TGAAAGAATT TATTCAGCAA 
CCAGAAAATA AGCTGGTACT AGTTAAACAA TTGGATAATA TCTTGGCTGC TGTACATGAC 
GTGCTTAATG AAAGTAGCAA ATTGCTTCAG GAGTTGAGAC AGGAGGGAGC TTGCTGTCTT 
GGCCTTCTTT GTGCTTCTCT GAGCTATGAG GCTGAGAAGA TCTTCAAGTG GATTTTTAGC 
AAATTTAGCT CATCTGCAAA AGATGAAGTT AAACTCCTCT ACTTATGTGC CACCTACAAA 
GCACTAGAGA CTGTAGGAGA AAAGAAAGCC TTTTCATCTG TAATGCAGCT TGTAATGACC 
AGCCTGCAGT CTATTCTTGA AAATGTGGAT ACACCAGAAT TGCTTTGTAA ATGTGTTAAG 
TGCATTCTTT TGGTGGCTCG ATGTTACCCT CATATTTTCA GCACTAATTT TAGGGATACA 
GTTGATATAT TAGTTGGATG GCATATAGAT CATACTCAGA AACCTTCGCT CACGCAGCAG 
GTATCTGGGT GGTTGCAGAG TTTGGAGCCA TTTTGGGTAG CTGATCTTGC ATTTTCTACT 
ACTCTTCTTG GTCAGTTTCT GGAAGACATG GAAGCATATG CTGAGGACCT CAGCCATGTG 
GCCTCTGGGG AATCAGTGGA TGAAGATGTC CCTCCTCCAT CAGTGTCATT ACCAAAGCTG 
GCTGCACTTC TCCGGGTATT TAGTACTGTG GTGAGGAGCA TTGGGGAACG CTTCAGCCCA 
ATTCGGGGTC CTCCAATTAC TGAGGCATAT GTAACAGATG TTCTGTACAG AGTAATGAGA 
TGTGTGACGG CTGCAAACCA GGTGTTTTTT TCTGAGGCTG TGTTGACAGC TGCTAATGAG 
TGTGTTGGTG TTTTGCTCGG CAGCTTGGAT CCTAGCATGA CTATACATTG TGACATGGTC 
ATTACATATG GATTAGACCA ACTGGAGAAT TGCCAGACTT GTGGTACCGA TTATATCATC 
TCAGTCTTGA ATTTACTCAC GCTGATTGTT GAACAGATAA ATACGAAACT GCCATCATCA 
TTTGTAGAAA AACTGTTTAT ACCATCATCT AAACTACTAT TCTTGCGTTA TCATAAAGAA 
AAAGAGGTTG TTGCTGTAGC CCATGCTGTT TATCAAGCAG TGCTCAGCTT GAAGAATATT 
CCTGTTTTGG AGACTGCCTA TAAGTTAATA TTGGGAGAAA TGACTTGTGC CCTAAACAAC 
CTCCTACACA GTCTACAACT TCCTGAGGCC TGTTCTGAAA TAAAACATGA GGCTTTTAAG 
AATCATGTGT TCAATGTAGA CAATGCAAAA TTTGTAGTTA TATTTGACCT CAGTGCCCTG 
ACTACAATTG GAAATGCCAA AAACTCACTA ATAGGGATGT GGGCGCTATC TCCAACTGTC 
TTTGCACTTC TGAGTAAGAA TCTGATGATT GTGCACAGTG ACCTGGCTGT TCACTTCCCT 
GCCATTCAGT ATGCTGTGCT CTACACATTG TATTCTCATT GTACCAGGCA TGATCACTTT 
ATCTCTAGTA GCCTCAGTTC TTCCTCTCCT TCTTTGTTTG ATGGAGCTGT GATTAGCACT 
GTAACTACGG CTACAAAGAA ACATTTCTCA ATTATATTAA ATCTTCTGGG AATATTACTT 
AAGAAAGATA ACCTTAACCA GGACACGAGG AAACTGTTAA TGACTTGGGC TTTGGAAGCA 
GCTGTTTTAA TGAAGAAGTC TGAAACATAC GCACCTTTAT TCTCTCTTCC GTCTTTCCAT 
AAATTTTGCA AAGGCCTTTT AGCCAACACT CTCGTTGAAG ATGTGAATAT CTGTCTGCAG 
GCATGCAGCA GTCTACATGC TCTGTCCTCT TCCTTGCCAG ATGATCTTTT ACAGAGATGT 
GTCGATGTTT GCCGTGTTCA ACTAGTGCAC AGTGGAACTC GTATTCGACA AGCATTTGGA 
AAACTGTTGA AATCAATTCC TTTAGATGTT GTCCTAAGCA ATAACAATCA CACAGAAATT 
CAAGAAATTT CTTTAGCATT AAGAAGTCAC ATGAGTAAAG CACCAAGTAA TACATTCCAC 
CCCCAAGATT TCTCTGATGT TATTAGTTTT ATTTTGTATG GGAACTCTCA TAGAACAGGG 
AAGGACAATT GGTTGGAAAG ACTGTTCTAT AGCTGCCAGA GACTGGATAA GCGTGACCAG 
TCAACAATTC CACGCAATCT CCTGAAGACA GATGCTGTCC TTTGGCAGTG GGCCATATGG 
GAAGCTGCAC AATTCACTGT TCTTTCTAAG CTGAGAACCC CACTGGGCAG AGCTCAAGAC 
ACCTTCCAGA CAATTGAAGG TATCATTCGA AGTCTCGCAG CTCACACATT AAACCCTGAT 
CAGGATGTTA GTCAGTGGAC AACTGCAGAC AATGATGAAG GCCATGGTAA CAACCAACTT 
AGACTTGTTC TTCTTCTGCA GTATCTGGAA AATCTGGAGA AATTAATGTA TAATGCATAC 
GAGGGATGTG CTAATGCATT AACTTCACCT CCCAAGGTCA TTAGAACTTT TTTCTATACC 
AATCGCCAAA CTTGTCAGGA CTGGCTAACG CGGATTCGAC TCTCCATCAT GAGGGTAGGA 
TTGTTGGCAG GCCAGCCTGC AGTGACAGTG AGACATGGCT TTGACTTGCT TACAGAGATG 
AAAACAACCA GCCTATCTCA GGGGAATGAA TTGGAAGTAA CCATTATGAT GGTGGTAGAA 
GCATTATGTG AACTTCATTG TCCTGAAGCT ATACAGGGAA TTGCTGTCTG GTCATCATCT 
ATTGTTGGAA AAAATCTTCT GTGGATTAAC TCAGTGGCTC AACAGGCTGA AGGGAGGTTT 
GAAAAGGCCT CTGTGGAGTA CCAGGAACAC CTGTGTGCCA TGACAGGTGT TGATTGCTGC 
ATCTCCAGCT TTGACAAATC GGTGCTCACC TTAGCCAATG CTGGGCGTAA CAGTGCCAGC 
CCGAAACATT CTCTGAATGG TGAATCCAGA AAAACTGTGC TGTCCAAACC GACTGACTCT 
TCCCCTGAGG TTATAAATTA TTTAGGAAAT AAAGCATGTG AGTGCTACAT CTCAATTGCC 
GATTGGGCTG CTGTGCAGGA ATGGCAGAAC GCTATCCATG ACTTGAAAAA GAGTACCAGT 
AGCACTTCCC TCAACCTGAA AGCTGACTTC AACTATATAA AATCATTAAG CAGCTTTGAG 
TCTGGAAAAT TTGTTGAATG TACCGAGCAG TTAGAATTGT TACCAGGAGA AAATATCAAT 
CTACTTGCTG GAGGATCAAA AGAAAAAATA GACATGAAAA AACTGCTTCC TAACATGTTA 
AGTCCGGATC CGAGGGAACT TCAGAAATCC ATTGAAGTTC AATTGTTAAG AAGTTCTGTT 
TGTTTGGCAA CTGCTTTAAA CCCGATAGAA CAAGATCAGA AGTGGCAGTC TATAACTGAA 
AATGTGGTAA AGTACTTGAA GCAAACATCC CGCATCGCTA TTGGACCTCT GAGACTTTCT 
ACTTTAACAG TTTCACAGTC TTTGCCAGTT CTAAGTACCT TGCAGCTGTA TTGCTCATCT 
GCTTTGGAGA ACACAGTTTC TAACAGACTT TCAACAGAGG ACTGTCTTAT TCCACTCTTC 
AGTGAAGCTT TACGTTCATG TAAACAGCAT GACGTGAGGC CATGGATGCA GGCATTAAGG 
TATACTATGT ACCAGAATCA GTTGTTGGAG AAAATTAAAG AACAAACAGT CCCAATTAGA 
AGCCATCTCA TGGAATTAGG TCTAACAGCA GCAAAATTTG CTAGAAAACG AGGGAATGTG 
TCCCTTGCAA CAAGACTGCT GGCACAGTGC AGTGAAGTTC AGCTGGGAAA GACCACCACT 
GCACAGGATT TAGTCCAACA TTTTAAAAAA CTATCAACCC AAGGTCAAGT GGATGAAAAA 
TGGGGGCCCG AACTTGATAT TGAAAAAACC AAATTGCTTT ATACAGCAGG CCAGTCAACA 
CATGCAATGG AAATGTTGAG TTCTTGTGCC ATATCTTTCT GCAAGTCTGT GAAAGCTGAA 
TATGCAGTTG CTAAATCAAT TCTGACACTG GCTAAATGGA TCCAGGCAGA ATGGAAAGAG 
ATTTCAGGAC AGCTGAAACA GGTTTACAGA GCTCAGCACC AACAGAACTT CACAGGTCTT 
TCTACTTTGT CTAAAAACAT ACTCACTCTA ATAGAACTGC CATCTGTTAA TACGATGGAA 
GAAGAGTATC CTCGGATCGA GAGTGAATCT ACAGTGCATA TTGGAGTTGG AGAACCTGAC 
TTCATTTTGG GACAGTTGTA TCACCTGTCT TCAGTACAGG CACCTGAAGT AGCCAAATCT 
TGGGCAGCGT TGGCCAGCTG GGCTTATAGG TGGGGCAGAA AGGTGGTTGA CAATGCCAGT 
CAGGGAGAAG GTGTTCGTCT GCTGCCTAGA GAAAAATCTG AAGTTCAGAA TCTACTTCCA 
GACACTATAA CTGAGGAAGA GAAAGAGAGA ATATATGGTA TTCTTGGACA GGCTGTGTGT 
CGGCCGGCGG GGATTCAGGA TGAAGATATA ACACTTCAGA TAACTGAGAG TGAAGACAAC 
GAAGAAGATG ACATGGTTGA TGTTATCTGG CGTCAGTTGA TATCAAGCTG CCCATGGCTT 
TCAGAACTTG ATGAAAGTGC AACTGAAGGA GTTATTAAAG TGTGGAGGAA AGTTGTAGAT 
AGAATATTCA GCCTGTACAA ACTCTCTTGC AGTGCATACT TTACTTTCCT TAAACTCAAC 
GCTGGTCAAA TTCCTTTAGA TGAGGATGAC CCTAGGCTGC ATTTAAGTCA CAGAGTGGAA 
CAGAGCACTG ATGACATGAT TGTGATGGCC ACATTGCGCC TGCTGCGGTT GCTCGTGAAG 
CATGCTGGTG AGCTTCGGCA GTATCTGGAG CACGGCTTGG AGACAACACC CACTGCACCA 
TGGAGAGGAA TTATTCCGCA ACTTTTCTCA CGCTTAAACC ACCCTGAAGT GTATGTGCGC 
CAAAGTATTT GTAACCTTCT CTGCCGTGTG GCTCAAGATT CCCCACATCT CATATTGTAT 
CCTGCAATAG TGGGTACCAT ATCGCTTAGT AGTGAATCCC AGGCTTCAGG AAATAAATTT 
TCCACTGCAA TTCCAACTTT ACTTGGCAAT ATTCAAGGAG AAGAATTGCT GGTTTCTGAA 
TGTGAGGGAG GAAGTCCTCC TGCATCTCAG GATAGCAATA AGGATGAACC TAAAAGTGGA 
TTAAATGAAG ACCAAGCCAT GATGCAGGAT TGTTACAGCA AAATTGTAGA TAAGCTGTCC 
TCTGCAAACC CCACCATGGT ATTACAGGTT CAGATGCTCG TGGCTGAACT GCGCAGGGTC 
ACTGTGCTCT GGGATGAGCT CTGGCTGGGA GTTTTGCTGC AACAACACAT GTATGTCCTG 
AGACGAATTC AGCAGCTTGA AGATGAGGTG AAGAGAGTCC AGAACAACAA CACCTTACGC 
AAAGAAGAGA AAATTGCAAT CATGAGGGAG AAGCACACAG CTTTGATGAA GCCCATCGTA 
TTTGCTTTGG AGCATGTGAG GAGTATCACA GCGGCTCCTG CAGAAACACC TCATGAAAAA 
TGGTTTCAGG ATAACTATGG TGATGCCATT GAAAATGCCC TAGAAAAACT GAAGACTCCA 
TTGAACCCTG CAAAGCCTGG GAGCAGCTGG ATTCCATTTA AAGAGATAAT GCTAAGTTTG 
CAACAGAGAG CACAGAAACG TGCAAGTTAC ATCTTGCGTC TTGAAGAAAT CAGTCCATGG 
TTGGCTGCCA TGACTAACAC TGAAATTGCT CTTCCTGGGG AAGTCTCAGC CAGAGACACT 
GTCACAATCC ATAGTGTGGG CGGAACCATC ACAATCTTAC CGACTAAAAC CAAGCCAAAG 
AAACTTCTCT TTCTTGGATC AGATGGGAAG AGCTATCCTT ATCTTTTCAA AGGACTGGAG 
GATTTACATC TGGATGAGAG AATAATGCAG TTCCTATCTA TTGTGAATAC CATGTTTGCT 
ACAATTAATC GCCAAGAAAC ACCCCGGTTC CATGCTCGAC ACTATTCTGT AACACCACTA 
GGAACAAGAT CAGGACTAAT CCAGTGGGTA GATGGAGCCA CACCCTTATT TGGTCTTTAC 
AAACGATGGC AACAACGGGA AGCTGCCTTA CAAGCACAAA AGGCCCAAGA TTCCTACCAA 
ACTCCTCAGA ATCCTGGAAT TGTACCCCGT CCTAGTGAAC TTTATTACAG TAAAATTGGC 
CCTGCTTTGA AAACAGTTGG GCTTAGCCTG GATGTGTCCC GTCGGGATTG GCCTCTTCAT 
GTAATGAAGG CAGTATTGGA AGAGTTAATG GAGGCCACAC CCCCGAATCT CCTTGCCAAA 
GAGCTCTGGT CATCTTGCAC AACACCTGAT GAATGGTGGA GAGTTACGCA GTCTTATGCA 
AGATCTACTG CAGTCATGTC TATGGTTGGA TACATAATTG GCCTTGGAGA CAGACATCTG 
GATAATGTTC TTATAGATAT GACGACTGGA GAAGTTGTTC ACATAGATTA CAATGTTTGC 
TTTGAAAAAG GTAATATAAA AGTAGACTTT ACATTTATGA GGGCCAAATG TTTTTTGGTG 
AAAGGGCAAA AATATAATGA TTTGTCTTTT CAAGGTAAAA GCCTTAGAGT TCCTGAGAAA 
GTACCTTTTC GAATGACACA AAACATTGAA ACAGCACTGG GTGTAACTGG AGTAGAAGGT 
GTATTTAGGC TTTCATGTGA GCAGGTTTTA CACATTATGC GGCGTGGCAG AGAGACCCTG 
CTGACGCTGC TGGAGGCCTT TGTGTACGAC CCTCTGGTGG ACTGGACAGC AGGAGGCGAG 
GCTGGGTTTG CTGGTGCTGT CTATGGTGGA GGTGGCCAGC AGGCCGAGAG CAAGCAGAGC 
AAGAGAGAGA TGGAGCGAGA GATCACCCGC AGCCTGTTTT CTTCTAGAGT AGCTGAGATT 
AAGGTGAACT GGTTTAAGAA TAGAGATGAG ATGCTGGTTG TGCTTCCCAA GTTGGACGGT 
AGCTTAGATG AATACCTAAG CTTGCAAGAG CAACTGACAG ATGTGGAAAA ACTGCAGGGC 
AAACTACTGG AGGAAATAGA GTTTCTAGAA GGAGCTGAAG GGGTGGATCA TCCTTCTCAT 
ACTCTGCAAC ACAGGTATTC TGAGCACACC CAACTACAGA CTCAGCAAAG AGCTGTTCAG 
GAAGCAATCC AGGTGAAGCT GAATGAATTT GAACAATGGA TAACACATTA TCAGGCTGCA 
TTCAATAATT TAGAAGCAAC ACAGCTTGCA AGCTTGCTTC AAGAGATAAG CACACAAATG 
GACCTTGGTC CTCCAAGTTA CGTGCCAGCA ACAGCCTTTC TGCAGAATGC TGGTCAGGCC 
CACTTGATTA GCCAGTGCGA GCAGCTGGAG GGGGAGGTTG GTGCTCTCCT GCAGCAGAGG 
CGCTCCGTGC TCCGTGGCTG TCTGGAGCAA CTGCATCACT ATGCAACCGT GGCCCTGCAG 
TATCCGAAGG CCATATTTCA GAAACATCGA ATTGAACAGT GGAAGACCTG GATGGAAGAG 
CTCATCTGTA ACACCACAGT AGAGCGTTGT CAAGAGCTCT ATAGGAAATA TGAAATGCAA 
TATGCTCCCC AGCCACCCCC AACAGTGTGT CAGTTCATCA CTGCCACTGA AATGACCCTG 
CAGCGATACG CAGCAGACAT CAACAGCAGA CTTATTAGAC AAGTGGAACG CTTGAAACAG 
GAAGCTGTCA CTGTGCCAGT TTGTGAAGAT CAGTTGAAAG AAATTGAACG TTGCATTAAA 
GTTTTCCTTC ATGAGAATGG AGAAGAAGGA TCTTTGAGTC TAGCAAGTGT TATTATTTCT 
GCCCTTTGTA CCCTTACAAG GCGTAACCTG ATGATGGAAG GTGCAGCGTC AAGTGCTGGA 
GAACAGCTGG TTGATCTGAC TTCTCGGGAT GGAGCCTGGT TCTTGGAGGA ACTCTGCAGT 
ATGAGCGGAA ACGTCACCTG CTTGGTTCAG TTACTGAAGC AGTGCCACCT GGTGCCACAG 
GACTTAGATA TCCCGAACCC CATGGAAGCG TCTGAGACAG TTCACTTAGC CAATGGAGTG 
TATACCTCAC TTCAGGAATT GAATTCGAAT TTCCGGCAAA TCATATTTCC AGAAGCACTT 
CGATGTTTAA TGAAAGGGGA ATACACGTTA GAAAGTATGC TGCATGAACT GGACGGTCTT 
ATTGAGCAGA CCACCGATGG CGTTCCCCTG CAGACTCTAG TGGAATCTCT TCAGGCCTAC 
TTAAGAAACG CAGCTATGGG ACTGGAAGAA GAAACACATG CTCATTACAT CGATGTTGCC 
AGACTACTAC ATGCTCAGTA CGGTGAATTA ATCCAACCGA GAAATGGTTC AGTTGATGAA 
ACACCCAAAA TGTCAGCTGG CCAGATGCTT TTGGTAGCAT TCGATGGCAT GTTTGCTCAA 
GTTGAAACTG CTTTCAGCTT ATTAGTTGAA AAGTTGAACA AGATGGAAAT TCCCATAGCT 
TGGCGAAAGA TTGACATCAT AAGGGAAGCC AGGAGTACTC AAGTTAATTT TTTTGATGAT 
GATAATCACC GGCAGGTGCT AGAAGAGATT TTCTTTCTAA AAAGACTACA GACTATTAAG 
GAGTTCTTCA GGCTCTGTGG TACCTTTTCT AAAACATTGT CAGGATCAAG TTCACTTGAA 
GATCAGAATA CTGTGAATGG GCCTGTACAG ATTGTCAATG TGAAAACCCT TTTTAGAAAC 
TCTTGTTTCA GTGAAGACCA AATGGCCAAA CCTATCAAGG CATTCACAGC TGACTTTGTG 
AGGCAGCTCT TGATAGGGCT ACCCAACCAA GCCCTCGGAC TCACACTGTG CAGTTTTATC 
AGTGCTCTGG GTGTAGACAT CATTGCTCAA GTAGAGGCAA AGGACTTTGG TGCCGAAAGC 
AAAGTTTCTG TTGATGATCT CTGTAAGAAA GCGGTGGAAC ATAACATCCA GATAGGGAAG 
TTCTCTCAGC TGGTTATGAA CAGGGCAACT GTGTTAGCAA GTTCTTACGA CACTGCCTGG 
AAGAAGCATG ACTTGGTGCG AAGGCTAGAA ACCAGTATTT CTTCTTGTAA GACAAGCCTG 
CAGCGGGTTC AGCTGCATAT TGCCATGTTT CAGTGGCAAC ATGAAGATCT ACTTATCAAT 
AGACCACAAG CCATGTCAGT CACACCTCCC CCACGGTCTG CTATCCTAAC CAGCATGAAA 
AAGAAGCTGC ATACCCTGAG CCAGATTGAA ACTTCTATTG CAACAGTTCA GGAGAAGCTA 
GCTGCACTTG AATCAAGTAT TGAACAGCGA CTCAAGTGGG CAGGTGGTGC CAACCCTGCA 
TTGGCCCCTG TACTACAAGA TTTTGAAGCA ACGATAGCTG AAAGAAGAAA TCTTGTCCTT 
AAAGAGAGCC AAAGAGCAAG TCAGGTCACA TTTCTCTGCA GCAATATCAT TCATTTTGAA 
AGTTTACGAA CAAGAACTGC AGAAGCCTTA AACCTGGATG CGGCGTTATT TGAACTAATC 
AAGCGATGTC AGCAGATGTG TTCGTTTGCA TCACAGTTTA ACAGTTCAGT GTCTGAGTTA 
GAGCTTCGTT TATTACAGAG AGTGGACACT GGTCTTGAAC ATCCTATTGG CAGCTCTGAA 
TGGCTTTTGT CAGCACACAA ACAGTTGACC CAGGATATGT CTACTCAGAG GGCAATTCAG 
ACAGAGAAAG AGCAGCAGAT AGAAACGGTC TGTGAAACAA TTCAGAATCT GGTTGATAAT 
ATAAAGACTG TGCTCACTGG TCATAACCGA CAGCTTGGAG ATGTCAAACA TCTCTTGAAA 
GCTATGGCTA AGGATGAAGA AGCTGCTCTG GCAGATGGTG AAGATGTTCC CTATGAGAAC 
AGTGTTAGGC AGTTTTTGGG TGAATATAAA TCATGGCAAG ACAACATTCA AACAGTTCTA 
TTTACATTAG TCCAGGCTAT GGGTCAGGTT CGAAGTCAAG AACACGTTGA AATGCTCCAG 
GAAATCACTC CCACCTTGAA AGAACTGAAA ACACAAAGTC AGAGTATCTA TAATAATTTA 
GTGAGTTTTG CATCACCCTT AGTCACCGAT GCAACAAATG AATGTTCGAG TCCAACGTCA 
TCTGCTACTT ATCAGCCATC CTTCGCTGCA GCAGTCCGGA GTAACACTGG CCAGAAGACT 
CAGCCTGATG TCATGTCACA GAATGCTAGA AAGCTGATCC AAAAAAATCT TGCTACATCA 
GCTGATACTC CACCAAGCAC CGTTCCAGGA ACTGGCAAGA GTGTTGCTTG TAGTCCTAAA 
AAGGCAGTCA GAGACCCTAA AACTGGGAAA GCGGTGCAAG AGAGAAACTC CTATGCAGTG 
AGTGTGTGGA AGAGAGTGAA AGCCAAGTTA GAGGGCCGAG ATGTTGATCC GAATAGGAGG 
ATGTCAGTTG CTGAACAGGT TGACTATGTC ATTAAGGAAG CAACTAATCT AGATAACTTG 
GCTCAGCTGT ATGAAGGTTG GACAGCCTGG GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_005255182.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255239.1 Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.

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CloneID	OHu49088
Clone ID Related Accession (Same CDS sequence)	XM_005255182.3
Accession Version	XM_005255182.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005255182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGGGTT AACAATGTGA CCAGCCCTGA GTTCACCAGT 
GTTCAGCATG GCAGTCGTGC TTTAGCCACC AAAGACATGA GGAAATCACA GGAGAGATCG 
ATGTCTTATT CTGATGAGTC TCGACTGTCG AATCTTCTTC GGAGGATCAC CCGGGAAGAC 
GACAGAGACC GAAGATTGGC TACTGTAAAG CAGTTGAAAG AATTTATTCA GCAACCAGAA 
AATAAGCTGG TACTAGTTAA ACAATTGGAT AATATCTTGG CTGCTGTACA TGACGTGCTT 
AATGAAAGTA GCAAATTGCT TCAGGAGTTG AGACAGGAGG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTGAGCTA TGAGGCTGAG AAGATCTTCA AGTGGATTTT TAGCAAATTT 
AGCTCATCTG CAAAAGATGA AGTTAAACTC CTCTACTTAT GTGCCACCTA CAAAGCACTA 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTGTAATGC AGCTTGTAAT GACCAGCCTG 
CAGTCTATTC TTGAAAATGT GGATACACCA GAATTGCTTT GTAAATGTGT TAAGTGCATT 
CTTTTGGTGG CTCGATGTTA CCCTCATATT TTCAGCACTA ATTTTAGGGA TACAGTTGAT 
ATATTAGTTG GATGGCATAT AGATCATACT CAGAAACCTT CGCTCACGCA GCAGGTATCT 
GGGTGGTTGC AGAGTTTGGA GCCATTTTGG GTAGCTGATC TTGCATTTTC TACTACTCTT 
CTTGGTCAGT TTCTGGAAGA CATGGAAGCA TATGCTGAGG ACCTCAGCCA TGTGGCCTCT 
GGGGAATCAG TGGATGAAGA TGTCCCTCCT CCATCAGTGT CATTACCAAA GCTGGCTGCA 
CTTCTCCGGG TATTTAGTAC TGTGGTGAGG AGCATTGGGG AACGCTTCAG CCCAATTCGG 
GGTCCTCCAA TTACTGAGGC ATATGTAACA GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACGGCTGCAA ACCAGGTGTT TTTTTCTGAG GCTGTGTTGA CAGCTGCTAA TGAGTGTGTT 
GGTGTTTTGC TCGGCAGCTT GGATCCTAGC ATGACTATAC ATTGTGACAT GGTCATTACA 
TATGGATTAG ACCAACTGGA GAATTGCCAG ACTTGTGGTA CCGATTATAT CATCTCAGTC 
TTGAATTTAC TCACGCTGAT TGTTGAACAG ATAAATACGA AACTGCCATC ATCATTTGTA 
GAAAAACTGT TTATACCATC ATCTAAACTA CTATTCTTGC GTTATCATAA AGAAAAAGAG 
GTTGTTGCTG TAGCCCATGC TGTTTATCAA GCAGTGCTCA GCTTGAAGAA TATTCCTGTT 
TTGGAGACTG CCTATAAGTT AATATTGGGA GAAATGACTT GTGCCCTAAA CAACCTCCTA 
CACAGTCTAC AACTTCCTGA GGCCTGTTCT GAAATAAAAC ATGAGGCTTT TAAGAATCAT 
GTGTTCAATG TAGACAATGC AAAATTTGTA GTTATATTTG ACCTCAGTGC CCTGACTACA 
ATTGGAAATG CCAAAAACTC ACTAATAGGG ATGTGGGCGC TATCTCCAAC TGTCTTTGCA 
CTTCTGAGTA AGAATCTGAT GATTGTGCAC AGTGACCTGG CTGTTCACTT CCCTGCCATT 
CAGTATGCTG TGCTCTACAC ATTGTATTCT CATTGTACCA GGCATGATCA CTTTATCTCT 
AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG TTTGATGGAG CTGTGATTAG CACTGTAACT 
ACGGCTACAA AGAAACATTT CTCAATTATA TTAAATCTTC TGGGAATATT ACTTAAGAAA 
GATAACCTTA ACCAGGACAC GAGGAAACTG TTAATGACTT GGGCTTTGGA AGCAGCTGTT 
TTAATGAAGA AGTCTGAAAC ATACGCACCT TTATTCTCTC TTCCGTCTTT CCATAAATTT 
TGCAAAGGCC TTTTAGCCAA CACTCTCGTT GAAGATGTGA ATATCTGTCT GCAGGCATGC 
AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG CCAGATGATC TTTTACAGAG ATGTGTCGAT 
GTTTGCCGTG TTCAACTAGT GCACAGTGGA ACTCGTATTC GACAAGCATT TGGAAAACTG 
TTGAAATCAA TTCCTTTAGA TGTTGTCCTA AGCAATAACA ATCACACAGA AATTCAAGAA 
ATTTCTTTAG CATTAAGAAG TCACATGAGT AAAGCACCAA GTAATACATT CCACCCCCAA 
GATTTCTCTG ATGTTATTAG TTTTATTTTG TATGGGAACT CTCATAGAAC AGGGAAGGAC 
AATTGGTTGG AAAGACTGTT CTATAGCTGC CAGAGACTGG ATAAGCGTGA CCAGTCAACA 
ATTCCACGCA ATCTCCTGAA GACAGATGCT GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT 
GCACAATTCA CTGTTCTTTC TAAGCTGAGA ACCCCACTGG GCAGAGCTCA AGACACCTTC 
CAGACAATTG AAGGTATCAT TCGAAGTCTC GCAGCTCACA CATTAAACCC TGATCAGGAT 
GTTAGTCAGT GGACAACTGC AGACAATGAT GAAGGCCATG GTAACAACCA ACTTAGACTT 
GTTCTTCTTC TGCAGTATCT GGAAAATCTG GAGAAATTAA TGTATAATGC ATACGAGGGA 
TGTGCTAATG CATTAACTTC ACCTCCCAAG GTCATTAGAA CTTTTTTCTA TACCAATCGC 
CAAACTTGTC AGGACTGGCT AACGCGGATT CGACTCTCCA TCATGAGGGT AGGATTGTTG 
GCAGGCCAGC CTGCAGTGAC AGTGAGACAT GGCTTTGACT TGCTTACAGA GATGAAAACA 
ACCAGCCTAT CTCAGGGGAA TGAATTGGAA GTAACCATTA TGATGGTGGT AGAAGCATTA 
TGTGAACTTC ATTGTCCTGA AGCTATACAG GGAATTGCTG TCTGGTCATC ATCTATTGTT 
GGAAAAAATC TTCTGTGGAT TAACTCAGTG GCTCAACAGG CTGAAGGGAG GTTTGAAAAG 
GCCTCTGTGG AGTACCAGGA ACACCTGTGT GCCATGACAG GTGTTGATTG CTGCATCTCC 
AGCTTTGACA AATCGGTGCT CACCTTAGCC AATGCTGGGC GTAACAGTGC CAGCCCGAAA 
CATTCTCTGA ATGGTGAATC CAGAAAAACT GTGCTGTCCA AACCGACTGA CTCTTCCCCT 
GAGGTTATAA ATTATTTAGG AAATAAAGCA TGTGAGTGCT ACATCTCAAT TGCCGATTGG 
GCTGCTGTGC AGGAATGGCA GAACGCTATC CATGACTTGA AAAAGAGTAC CAGTAGCACT 
TCCCTCAACC TGAAAGCTGA CTTCAACTAT ATAAAATCAT TAAGCAGCTT TGAGTCTGGA 
AAATTTGTTG AATGTACCGA GCAGTTAGAA TTGTTACCAG GAGAAAATAT CAATCTACTT 
GCTGGAGGAT CAAAAGAAAA AATAGACATG AAAAAACTGC TTCCTAACAT GTTAAGTCCG 
GATCCGAGGG AACTTCAGAA ATCCATTGAA GTTCAATTGT TAAGAAGTTC TGTTTGTTTG 
GCAACTGCTT TAAACCCGAT AGAACAAGAT CAGAAGTGGC AGTCTATAAC TGAAAATGTG 
GTAAAGTACT TGAAGCAAAC ATCCCGCATC GCTATTGGAC CTCTGAGACT TTCTACTTTA 
ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT ACCTTGCAGC TGTATTGCTC ATCTGCTTTG 
GAGAACACAG TTTCTAACAG ACTTTCAACA GAGGACTGTC TTATTCCACT CTTCAGTGAA 
GCTTTACGTT CATGTAAACA GCATGACGTG AGGCCATGGA TGCAGGCATT AAGGTATACT 
ATGTACCAGA ATCAGTTGTT GGAGAAAATT AAAGAACAAA CAGTCCCAAT TAGAAGCCAT 
CTCATGGAAT TAGGTCTAAC AGCAGCAAAA TTTGCTAGAA AACGAGGGAA TGTGTCCCTT 
GCAACAAGAC TGCTGGCACA GTGCAGTGAA GTTCAGCTGG GAAAGACCAC CACTGCACAG 
GATTTAGTCC AACATTTTAA AAAACTATCA ACCCAAGGTC AAGTGGATGA AAAATGGGGG 
CCCGAACTTG ATATTGAAAA AACCAAATTG CTTTATACAG CAGGCCAGTC AACACATGCA 
ATGGAAATGT TGAGTTCTTG TGCCATATCT TTCTGCAAGT CTGTGAAAGC TGAATATGCA 
GTTGCTAAAT CAATTCTGAC ACTGGCTAAA TGGATCCAGG CAGAATGGAA AGAGATTTCA 
GGACAGCTGA AACAGGTTTA CAGAGCTCAG CACCAACAGA ACTTCACAGG TCTTTCTACT 
TTGTCTAAAA ACATACTCAC TCTAATAGAA CTGCCATCTG TTAATACGAT GGAAGAAGAG 
TATCCTCGGA TCGAGAGTGA ATCTACAGTG CATATTGGAG TTGGAGAACC TGACTTCATT 
TTGGGACAGT TGTATCACCT GTCTTCAGTA CAGGCACCTG AAGTAGCCAA ATCTTGGGCA 
GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC AGAAAGGTGG TTGACAATGC CAGTCAGGGA 
GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA TCTGAAGTTC AGAATCTACT TCCAGACACT 
ATAACTGAGG AAGAGAAAGA GAGAATATAT GGTATTCTTG GACAGGCTGT GTGTCGGCCG 
GCGGGGATTC AGGATGAAGA TATAACACTT CAGATAACTG AGAGTGAAGA CAACGAAGAA 
GATGACATGG TTGATGTTAT CTGGCGTCAG TTGATATCAA GCTGCCCATG GCTTTCAGAA 
CTTGATGAAA GTGCAACTGA AGGAGTTATT AAAGTGTGGA GGAAAGTTGT AGATAGAATA 
TTCAGCCTGT ACAAACTCTC TTGCAGTGCA TACTTTACTT TCCTTAAACT CAACGCTGGT 
CAAATTCCTT TAGATGAGGA TGACCCTAGG CTGCATTTAA GTCACAGAGT GGAACAGAGC 
ACTGATGACA TGATTGTGAT GGCCACATTG CGCCTGCTGC GGTTGCTCGT GAAGCATGCT 
GGTGAGCTTC GGCAGTATCT GGAGCACGGC TTGGAGACAA CACCCACTGC ACCATGGAGA 
GGAATTATTC CGCAACTTTT CTCACGCTTA AACCACCCTG AAGTGTATGT GCGCCAAAGT 
ATTTGTAACC TTCTCTGCCG TGTGGCTCAA GATTCCCCAC ATCTCATATT GTATCCTGCA 
ATAGTGGGTA CCATATCGCT TAGTAGTGAA TCCCAGGCTT CAGGAAATAA ATTTTCCACT 
GCAATTCCAA CTTTACTTGG CAATATTCAA GGAGAAGAAT TGCTGGTTTC TGAATGTGAG 
GGAGGAAGTC CTCCTGCATC TCAGGATAGC AATAAGGATG AACCTAAAAG TGGATTAAAT 
GAAGACCAAG CCATGATGCA GGATTGTTAC AGCAAAATTG TAGATAAGCT GTCCTCTGCA 
AACCCCACCA TGGTATTACA GGTTCAGATG CTCGTGGCTG AACTGCGCAG GGTCACTGTG 
CTCTGGGATG AGCTCTGGCT GGGAGTTTTG CTGCAACAAC ACATGTATGT CCTGAGACGA 
ATTCAGCAGC TTGAAGATGA GGTGAAGAGA GTCCAGAACA ACAACACCTT ACGCAAAGAA 
GAGAAAATTG CAATCATGAG GGAGAAGCAC ACAGCTTTGA TGAAGCCCAT CGTATTTGCT 
TTGGAGCATG TGAGGAGTAT CACAGCGGCT CCTGCAGAAA CACCTCATGA AAAATGGTTT 
CAGGATAACT ATGGTGATGC CATTGAAAAT GCCCTAGAAA AACTGAAGAC TCCATTGAAC 
CCTGCAAAGC CTGGGAGCAG CTGGATTCCA TTTAAAGAGA TAATGCTAAG TTTGCAACAG 
AGAGCACAGA AACGTGCAAG TTACATCTTG CGTCTTGAAG AAATCAGTCC ATGGTTGGCT 
GCCATGACTA ACACTGAAAT TGCTCTTCCT GGGGAAGTCT CAGCCAGAGA CACTGTCACA 
ATCCATAGTG TGGGCGGAAC CATCACAATC TTACCGACTA AAACCAAGCC AAAGAAACTT 
CTCTTTCTTG GATCAGATGG GAAGAGCTAT CCTTATCTTT TCAAAGGACT GGAGGATTTA 
CATCTGGATG AGAGAATAAT GCAGTTCCTA TCTATTGTGA ATACCATGTT TGCTACAATT 
AATCGCCAAG AAACACCCCG GTTCCATGCT CGACACTATT CTGTAACACC ACTAGGAACA 
AGATCAGGAC TAATCCAGTG GGTAGATGGA GCCACACCCT TATTTGGTCT TTACAAACGA 
TGGCAACAAC GGGAAGCTGC CTTACAAGCA CAAAAGGCCC AAGATTCCTA CCAAACTCCT 
CAGAATCCTG GAATTGTACC CCGTCCTAGT GAACTTTATT ACAGTAAAAT TGGCCCTGCT 
TTGAAAACAG TTGGGCTTAG CCTGGATGTG TCCCGTCGGG ATTGGCCTCT TCATGTAATG 
AAGGCAGTAT TGGAAGAGTT AATGGAGGCC ACACCCCCGA ATCTCCTTGC CAAAGAGCTC 
TGGTCATCTT GCACAACACC TGATGAATGG TGGAGAGTTA CGCAGTCTTA TGCAAGATCT 
ACTGCAGTCA TGTCTATGGT TGGATACATA ATTGGCCTTG GAGACAGACA TCTGGATAAT 
GTTCTTATAG ATATGACGAC TGGAGAAGTT GTTCACATAG ATTACAATGT TTGCTTTGAA 
AAAGGTAATA TAAAAGTAGA CTTTACATTT ATGAGGGCCA AATGTTTTTT GGTGAAAGGG 
CAAAAATATA ATGATTTGTC TTTTCAAGGT AAAAGCCTTA GAGTTCCTGA GAAAGTACCT 
TTTCGAATGA CACAAAACAT TGAAACAGCA CTGGGTGTAA CTGGAGTAGA AGGTGTATTT 
AGGCTTTCAT GTGAGCAGGT TTTACACATT ATGCGGCGTG GCAGAGAGAC CCTGCTGACG 
CTGCTGGAGG CCTTTGTGTA CGACCCTCTG GTGGACTGGA CAGCAGGAGG CGAGGCTGGG 
TTTGCTGGTG CTGTCTATGG TGGAGGTGGC CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA 
GAGATGGAGC GAGAGATCAC CCGCAGCCTG TTTTCTTCTA GAGTAGCTGA GATTAAGGTG 
AACTGGTTTA AGAATAGAGA TGAGATGCTG GTTGTGCTTC CCAAGTTGGA CGGTAGCTTA 
GATGAATACC TAAGCTTGCA AGAGCAACTG ACAGATGTGG AAAAACTGCA GGGCAAACTA 
CTGGAGGAAA TAGAGTTTCT AGAAGGAGCT GAAGGGGTGG ATCATCCTTC TCATACTCTG 
CAACACAGGT ATTCTGAGCA CACCCAACTA CAGACTCAGC AAAGAGCTGT TCAGGAAGCA 
ATCCAGGTGA AGCTGAATGA ATTTGAACAA TGGATAACAC ATTATCAGGC TGCATTCAAT 
AATTTAGAAG CAACACAGCT TGCAAGCTTG CTTCAAGAGA TAAGCACACA AATGGACCTT 
GGTCCTCCAA GTTACGTGCC AGCAACAGCC TTTCTGCAGA ATGCTGGTCA GGCCCACTTG 
ATTAGCCAGT GCGAGCAGCT GGAGGGGGAG GTTGGTGCTC TCCTGCAGCA GAGGCGCTCC 
GTGCTCCGTG GCTGTCTGGA GCAACTGCAT CACTATGCAA CCGTGGCCCT GCAGTATCCG 
AAGGCCATAT TTCAGAAACA TCGAATTGAA CAGTGGAAGA CCTGGATGGA AGAGCTCATC 
TGTAACACCA CAGTAGAGCG TTGTCAAGAG CTCTATAGGA AATATGAAAT GCAATATGCT 
CCCCAGCCAC CCCCAACAGT GTGTCAGTTC ATCACTGCCA CTGAAATGAC CCTGCAGCGA 
TACGCAGCAG ACATCAACAG CAGACTTATT AGACAAGTGG AACGCTTGAA ACAGGAAGCT 
GTCACTGTGC CAGTTTGTGA AGATCAGTTG AAAGAAATTG AACGTTGCAT TAAAGTTTTC 
CTTCATGAGA ATGGAGAAGA AGGATCTTTG AGTCTAGCAA GTGTTATTAT TTCTGCCCTT 
TGTACCCTTA CAAGGCGTAA CCTGATGATG GAAGGTGCAG CGTCAAGTGC TGGAGAACAG 
CTGGTTGATC TGACTTCTCG GGATGGAGCC TGGTTCTTGG AGGAACTCTG CAGTATGAGC 
GGAAACGTCA CCTGCTTGGT TCAGTTACTG AAGCAGTGCC ACCTGGTGCC ACAGGACTTA 
GATATCCCGA ACCCCATGGA AGCGTCTGAG ACAGTTCACT TAGCCAATGG AGTGTATACC 
TCACTTCAGG AATTGAATTC GAATTTCCGG CAAATCATAT TTCCAGAAGC ACTTCGATGT 
TTAATGAAAG GGGAATACAC GTTAGAAAGT ATGCTGCATG AACTGGACGG TCTTATTGAG 
CAGACCACCG ATGGCGTTCC CCTGCAGACT CTAGTGGAAT CTCTTCAGGC CTACTTAAGA 
AACGCAGCTA TGGGACTGGA AGAAGAAACA CATGCTCATT ACATCGATGT TGCCAGACTA 
CTACATGCTC AGTACGGTGA ATTAATCCAA CCGAGAAATG GTTCAGTTGA TGAAACACCC 
AAAATGTCAG CTGGCCAGAT GCTTTTGGTA GCATTCGATG GCATGTTTGC TCAAGTTGAA 
ACTGCTTTCA GCTTATTAGT TGAAAAGTTG AACAAGATGG AAATTCCCAT AGCTTGGCGA 
AAGATTGACA TCATAAGGGA AGCCAGGAGT ACTCAAGTTA ATTTTTTTGA TGATGATAAT 
CACCGGCAGG TGCTAGAAGA GATTTTCTTT CTAAAAAGAC TACAGACTAT TAAGGAGTTC 
TTCAGGCTCT GTGGTACCTT TTCTAAAACA TTGTCAGGAT CAAGTTCACT TGAAGATCAG 
AATACTGTGA ATGGGCCTGT ACAGATTGTC AATGTGAAAA CCCTTTTTAG AAACTCTTGT 
TTCAGTGAAG ACCAAATGGC CAAACCTATC AAGGCATTCA CAGCTGACTT TGTGAGGCAG 
CTCTTGATAG GGCTACCCAA CCAAGCCCTC GGACTCACAC TGTGCAGTTT TATCAGTGCT 
CTGGGTGTAG ACATCATTGC TCAAGTAGAG GCAAAGGACT TTGGTGCCGA AAGCAAAGTT 
TCTGTTGATG ATCTCTGTAA GAAAGCGGTG GAACATAACA TCCAGATAGG GAAGTTCTCT 
CAGCTGGTTA TGAACAGGGC AACTGTGTTA GCAAGTTCTT ACGACACTGC CTGGAAGAAG 
CATGACTTGG TGCGAAGGCT AGAAACCAGT ATTTCTTCTT GTAAGACAAG CCTGCAGCGG 
GTTCAGCTGC ATATTGCCAT GTTTCAGTGG CAACATGAAG ATCTACTTAT CAATAGACCA 
CAAGCCATGT CAGTCACACC TCCCCCACGG TCTGCTATCC TAACCAGCAT GAAAAAGAAG 
CTGCATACCC TGAGCCAGAT TGAAACTTCT ATTGCAACAG TTCAGGAGAA GCTAGCTGCA 
CTTGAATCAA GTATTGAACA GCGACTCAAG TGGGCAGGTG GTGCCAACCC TGCATTGGCC 
CCTGTACTAC AAGATTTTGA AGCAACGATA GCTGAAAGAA GAAATCTTGT CCTTAAAGAG 
AGCCAAAGAG CAAGTCAGGT CACATTTCTC TGCAGCAATA TCATTCATTT TGAAAGTTTA 
CGAACAAGAA CTGCAGAAGC CTTAAACCTG GATGCGGCGT TATTTGAACT AATCAAGCGA 
TGTCAGCAGA TGTGTTCGTT TGCATCACAG TTTAACAGTT CAGTGTCTGA GTTAGAGCTT 
CGTTTATTAC AGAGAGTGGA CACTGGTCTT GAACATCCTA TTGGCAGCTC TGAATGGCTT 
TTGTCAGCAC ACAAACAGTT GACCCAGGAT ATGTCTACTC AGAGGGCAAT TCAGACAGAG 
AAAGAGCAGC AGATAGAAAC GGTCTGTGAA ACAATTCAGA ATCTGGTTGA TAATATAAAG 
ACTGTGCTCA CTGGTCATAA CCGACAGCTT GGAGATGTCA AACATCTCTT GAAAGCTATG 
GCTAAGGATG AAGAAGCTGC TCTGGCAGAT GGTGAAGATG TTCCCTATGA GAACAGTGTT 
AGGCAGTTTT TGGGTGAATA TAAATCATGG CAAGACAACA TTCAAACAGT TCTATTTACA 
TTAGTCCAGG CTATGGGTCA GGTTCGAAGT CAAGAACACG TTGAAATGCT CCAGGAAATC 
ACTCCCACCT TGAAAGAACT GAAAACACAA AGTCAGAGTA TCTATAATAA TTTAGTGAGT 
TTTGCATCAC CCTTAGTCAC CGATGCAACA AATGAATGTT CGAGTCCAAC GTCATCTGCT 
ACTTATCAGC CATCCTTCGC TGCAGCAGTC CGGAGTAACA CTGGCCAGAA GACTCAGCCT 
GATGTCATGT CACAGAATGC TAGAAAGCTG ATCCAAAAAA ATCTTGCTAC ATCAGCTGAT 
ACTCCACCAA GCACCGTTCC AGGAACTGGC AAGAGTGTTG CTTGTAGTCC TAAAAAGGCA 
GTCAGAGACC CTAAAACTGG GAAAGCGGTG CAAGAGAGAA ACTCCTATGC AGTGAGTGTG 
TGGAAGAGAG TGAAAGCCAA GTTAGAGGGC CGAGATGTTG ATCCGAATAG GAGGATGTCA 
GTTGCTGAAC AGGTTGACTA TGTCATTAAG GAAGCAACTA ATCTAGATAA CTTGGCTCAG 
CTGTATGAAG GTTGGACAGC CTGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255239.1
CDS	377..11446
Translation	MSRRAPGSRLSSGGGGGGTKYPRSWNDWQPRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSVQHGSRALATKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGNIKVDFTFMRAKCFLVKGQKYNDLSFQGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_005255182.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255182.3

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGGGTT AACAATGTGA CCAGCCCTGA GTTCACCAGT 
GTTCAGCATG GCAGTCGTGC TTTAGCCACC AAAGACATGA GGAAATCACA GGAGAGATCG 
ATGTCTTATT CTGATGAGTC TCGACTGTCG AATCTTCTTC GGAGGATCAC CCGGGAAGAC 
GACAGAGACC GAAGATTGGC TACTGTAAAG CAGTTGAAAG AATTTATTCA GCAACCAGAA 
AATAAGCTGG TACTAGTTAA ACAATTGGAT AATATCTTGG CTGCTGTACA TGACGTGCTT 
AATGAAAGTA GCAAATTGCT TCAGGAGTTG AGACAGGAGG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTGAGCTA TGAGGCTGAG AAGATCTTCA AGTGGATTTT TAGCAAATTT 
AGCTCATCTG CAAAAGATGA AGTTAAACTC CTCTACTTAT GTGCCACCTA CAAAGCACTA 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTGTAATGC AGCTTGTAAT GACCAGCCTG 
CAGTCTATTC TTGAAAATGT GGATACACCA GAATTGCTTT GTAAATGTGT TAAGTGCATT 
CTTTTGGTGG CTCGATGTTA CCCTCATATT TTCAGCACTA ATTTTAGGGA TACAGTTGAT 
ATATTAGTTG GATGGCATAT AGATCATACT CAGAAACCTT CGCTCACGCA GCAGGTATCT 
GGGTGGTTGC AGAGTTTGGA GCCATTTTGG GTAGCTGATC TTGCATTTTC TACTACTCTT 
CTTGGTCAGT TTCTGGAAGA CATGGAAGCA TATGCTGAGG ACCTCAGCCA TGTGGCCTCT 
GGGGAATCAG TGGATGAAGA TGTCCCTCCT CCATCAGTGT CATTACCAAA GCTGGCTGCA 
CTTCTCCGGG TATTTAGTAC TGTGGTGAGG AGCATTGGGG AACGCTTCAG CCCAATTCGG 
GGTCCTCCAA TTACTGAGGC ATATGTAACA GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACGGCTGCAA ACCAGGTGTT TTTTTCTGAG GCTGTGTTGA CAGCTGCTAA TGAGTGTGTT 
GGTGTTTTGC TCGGCAGCTT GGATCCTAGC ATGACTATAC ATTGTGACAT GGTCATTACA 
TATGGATTAG ACCAACTGGA GAATTGCCAG ACTTGTGGTA CCGATTATAT CATCTCAGTC 
TTGAATTTAC TCACGCTGAT TGTTGAACAG ATAAATACGA AACTGCCATC ATCATTTGTA 
GAAAAACTGT TTATACCATC ATCTAAACTA CTATTCTTGC GTTATCATAA AGAAAAAGAG 
GTTGTTGCTG TAGCCCATGC TGTTTATCAA GCAGTGCTCA GCTTGAAGAA TATTCCTGTT 
TTGGAGACTG CCTATAAGTT AATATTGGGA GAAATGACTT GTGCCCTAAA CAACCTCCTA 
CACAGTCTAC AACTTCCTGA GGCCTGTTCT GAAATAAAAC ATGAGGCTTT TAAGAATCAT 
GTGTTCAATG TAGACAATGC AAAATTTGTA GTTATATTTG ACCTCAGTGC CCTGACTACA 
ATTGGAAATG CCAAAAACTC ACTAATAGGG ATGTGGGCGC TATCTCCAAC TGTCTTTGCA 
CTTCTGAGTA AGAATCTGAT GATTGTGCAC AGTGACCTGG CTGTTCACTT CCCTGCCATT 
CAGTATGCTG TGCTCTACAC ATTGTATTCT CATTGTACCA GGCATGATCA CTTTATCTCT 
AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG TTTGATGGAG CTGTGATTAG CACTGTAACT 
ACGGCTACAA AGAAACATTT CTCAATTATA TTAAATCTTC TGGGAATATT ACTTAAGAAA 
GATAACCTTA ACCAGGACAC GAGGAAACTG TTAATGACTT GGGCTTTGGA AGCAGCTGTT 
TTAATGAAGA AGTCTGAAAC ATACGCACCT TTATTCTCTC TTCCGTCTTT CCATAAATTT 
TGCAAAGGCC TTTTAGCCAA CACTCTCGTT GAAGATGTGA ATATCTGTCT GCAGGCATGC 
AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG CCAGATGATC TTTTACAGAG ATGTGTCGAT 
GTTTGCCGTG TTCAACTAGT GCACAGTGGA ACTCGTATTC GACAAGCATT TGGAAAACTG 
TTGAAATCAA TTCCTTTAGA TGTTGTCCTA AGCAATAACA ATCACACAGA AATTCAAGAA 
ATTTCTTTAG CATTAAGAAG TCACATGAGT AAAGCACCAA GTAATACATT CCACCCCCAA 
GATTTCTCTG ATGTTATTAG TTTTATTTTG TATGGGAACT CTCATAGAAC AGGGAAGGAC 
AATTGGTTGG AAAGACTGTT CTATAGCTGC CAGAGACTGG ATAAGCGTGA CCAGTCAACA 
ATTCCACGCA ATCTCCTGAA GACAGATGCT GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT 
GCACAATTCA CTGTTCTTTC TAAGCTGAGA ACCCCACTGG GCAGAGCTCA AGACACCTTC 
CAGACAATTG AAGGTATCAT TCGAAGTCTC GCAGCTCACA CATTAAACCC TGATCAGGAT 
GTTAGTCAGT GGACAACTGC AGACAATGAT GAAGGCCATG GTAACAACCA ACTTAGACTT 
GTTCTTCTTC TGCAGTATCT GGAAAATCTG GAGAAATTAA TGTATAATGC ATACGAGGGA 
TGTGCTAATG CATTAACTTC ACCTCCCAAG GTCATTAGAA CTTTTTTCTA TACCAATCGC 
CAAACTTGTC AGGACTGGCT AACGCGGATT CGACTCTCCA TCATGAGGGT AGGATTGTTG 
GCAGGCCAGC CTGCAGTGAC AGTGAGACAT GGCTTTGACT TGCTTACAGA GATGAAAACA 
ACCAGCCTAT CTCAGGGGAA TGAATTGGAA GTAACCATTA TGATGGTGGT AGAAGCATTA 
TGTGAACTTC ATTGTCCTGA AGCTATACAG GGAATTGCTG TCTGGTCATC ATCTATTGTT 
GGAAAAAATC TTCTGTGGAT TAACTCAGTG GCTCAACAGG CTGAAGGGAG GTTTGAAAAG 
GCCTCTGTGG AGTACCAGGA ACACCTGTGT GCCATGACAG GTGTTGATTG CTGCATCTCC 
AGCTTTGACA AATCGGTGCT CACCTTAGCC AATGCTGGGC GTAACAGTGC CAGCCCGAAA 
CATTCTCTGA ATGGTGAATC CAGAAAAACT GTGCTGTCCA AACCGACTGA CTCTTCCCCT 
GAGGTTATAA ATTATTTAGG AAATAAAGCA TGTGAGTGCT ACATCTCAAT TGCCGATTGG 
GCTGCTGTGC AGGAATGGCA GAACGCTATC CATGACTTGA AAAAGAGTAC CAGTAGCACT 
TCCCTCAACC TGAAAGCTGA CTTCAACTAT ATAAAATCAT TAAGCAGCTT TGAGTCTGGA 
AAATTTGTTG AATGTACCGA GCAGTTAGAA TTGTTACCAG GAGAAAATAT CAATCTACTT 
GCTGGAGGAT CAAAAGAAAA AATAGACATG AAAAAACTGC TTCCTAACAT GTTAAGTCCG 
GATCCGAGGG AACTTCAGAA ATCCATTGAA GTTCAATTGT TAAGAAGTTC TGTTTGTTTG 
GCAACTGCTT TAAACCCGAT AGAACAAGAT CAGAAGTGGC AGTCTATAAC TGAAAATGTG 
GTAAAGTACT TGAAGCAAAC ATCCCGCATC GCTATTGGAC CTCTGAGACT TTCTACTTTA 
ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT ACCTTGCAGC TGTATTGCTC ATCTGCTTTG 
GAGAACACAG TTTCTAACAG ACTTTCAACA GAGGACTGTC TTATTCCACT CTTCAGTGAA 
GCTTTACGTT CATGTAAACA GCATGACGTG AGGCCATGGA TGCAGGCATT AAGGTATACT 
ATGTACCAGA ATCAGTTGTT GGAGAAAATT AAAGAACAAA CAGTCCCAAT TAGAAGCCAT 
CTCATGGAAT TAGGTCTAAC AGCAGCAAAA TTTGCTAGAA AACGAGGGAA TGTGTCCCTT 
GCAACAAGAC TGCTGGCACA GTGCAGTGAA GTTCAGCTGG GAAAGACCAC CACTGCACAG 
GATTTAGTCC AACATTTTAA AAAACTATCA ACCCAAGGTC AAGTGGATGA AAAATGGGGG 
CCCGAACTTG ATATTGAAAA AACCAAATTG CTTTATACAG CAGGCCAGTC AACACATGCA 
ATGGAAATGT TGAGTTCTTG TGCCATATCT TTCTGCAAGT CTGTGAAAGC TGAATATGCA 
GTTGCTAAAT CAATTCTGAC ACTGGCTAAA TGGATCCAGG CAGAATGGAA AGAGATTTCA 
GGACAGCTGA AACAGGTTTA CAGAGCTCAG CACCAACAGA ACTTCACAGG TCTTTCTACT 
TTGTCTAAAA ACATACTCAC TCTAATAGAA CTGCCATCTG TTAATACGAT GGAAGAAGAG 
TATCCTCGGA TCGAGAGTGA ATCTACAGTG CATATTGGAG TTGGAGAACC TGACTTCATT 
TTGGGACAGT TGTATCACCT GTCTTCAGTA CAGGCACCTG AAGTAGCCAA ATCTTGGGCA 
GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC AGAAAGGTGG TTGACAATGC CAGTCAGGGA 
GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA TCTGAAGTTC AGAATCTACT TCCAGACACT 
ATAACTGAGG AAGAGAAAGA GAGAATATAT GGTATTCTTG GACAGGCTGT GTGTCGGCCG 
GCGGGGATTC AGGATGAAGA TATAACACTT CAGATAACTG AGAGTGAAGA CAACGAAGAA 
GATGACATGG TTGATGTTAT CTGGCGTCAG TTGATATCAA GCTGCCCATG GCTTTCAGAA 
CTTGATGAAA GTGCAACTGA AGGAGTTATT AAAGTGTGGA GGAAAGTTGT AGATAGAATA 
TTCAGCCTGT ACAAACTCTC TTGCAGTGCA TACTTTACTT TCCTTAAACT CAACGCTGGT 
CAAATTCCTT TAGATGAGGA TGACCCTAGG CTGCATTTAA GTCACAGAGT GGAACAGAGC 
ACTGATGACA TGATTGTGAT GGCCACATTG CGCCTGCTGC GGTTGCTCGT GAAGCATGCT 
GGTGAGCTTC GGCAGTATCT GGAGCACGGC TTGGAGACAA CACCCACTGC ACCATGGAGA 
GGAATTATTC CGCAACTTTT CTCACGCTTA AACCACCCTG AAGTGTATGT GCGCCAAAGT 
ATTTGTAACC TTCTCTGCCG TGTGGCTCAA GATTCCCCAC ATCTCATATT GTATCCTGCA 
ATAGTGGGTA CCATATCGCT TAGTAGTGAA TCCCAGGCTT CAGGAAATAA ATTTTCCACT 
GCAATTCCAA CTTTACTTGG CAATATTCAA GGAGAAGAAT TGCTGGTTTC TGAATGTGAG 
GGAGGAAGTC CTCCTGCATC TCAGGATAGC AATAAGGATG AACCTAAAAG TGGATTAAAT 
GAAGACCAAG CCATGATGCA GGATTGTTAC AGCAAAATTG TAGATAAGCT GTCCTCTGCA 
AACCCCACCA TGGTATTACA GGTTCAGATG CTCGTGGCTG AACTGCGCAG GGTCACTGTG 
CTCTGGGATG AGCTCTGGCT GGGAGTTTTG CTGCAACAAC ACATGTATGT CCTGAGACGA 
ATTCAGCAGC TTGAAGATGA GGTGAAGAGA GTCCAGAACA ACAACACCTT ACGCAAAGAA 
GAGAAAATTG CAATCATGAG GGAGAAGCAC ACAGCTTTGA TGAAGCCCAT CGTATTTGCT 
TTGGAGCATG TGAGGAGTAT CACAGCGGCT CCTGCAGAAA CACCTCATGA AAAATGGTTT 
CAGGATAACT ATGGTGATGC CATTGAAAAT GCCCTAGAAA AACTGAAGAC TCCATTGAAC 
CCTGCAAAGC CTGGGAGCAG CTGGATTCCA TTTAAAGAGA TAATGCTAAG TTTGCAACAG 
AGAGCACAGA AACGTGCAAG TTACATCTTG CGTCTTGAAG AAATCAGTCC ATGGTTGGCT 
GCCATGACTA ACACTGAAAT TGCTCTTCCT GGGGAAGTCT CAGCCAGAGA CACTGTCACA 
ATCCATAGTG TGGGCGGAAC CATCACAATC TTACCGACTA AAACCAAGCC AAAGAAACTT 
CTCTTTCTTG GATCAGATGG GAAGAGCTAT CCTTATCTTT TCAAAGGACT GGAGGATTTA 
CATCTGGATG AGAGAATAAT GCAGTTCCTA TCTATTGTGA ATACCATGTT TGCTACAATT 
AATCGCCAAG AAACACCCCG GTTCCATGCT CGACACTATT CTGTAACACC ACTAGGAACA 
AGATCAGGAC TAATCCAGTG GGTAGATGGA GCCACACCCT TATTTGGTCT TTACAAACGA 
TGGCAACAAC GGGAAGCTGC CTTACAAGCA CAAAAGGCCC AAGATTCCTA CCAAACTCCT 
CAGAATCCTG GAATTGTACC CCGTCCTAGT GAACTTTATT ACAGTAAAAT TGGCCCTGCT 
TTGAAAACAG TTGGGCTTAG CCTGGATGTG TCCCGTCGGG ATTGGCCTCT TCATGTAATG 
AAGGCAGTAT TGGAAGAGTT AATGGAGGCC ACACCCCCGA ATCTCCTTGC CAAAGAGCTC 
TGGTCATCTT GCACAACACC TGATGAATGG TGGAGAGTTA CGCAGTCTTA TGCAAGATCT 
ACTGCAGTCA TGTCTATGGT TGGATACATA ATTGGCCTTG GAGACAGACA TCTGGATAAT 
GTTCTTATAG ATATGACGAC TGGAGAAGTT GTTCACATAG ATTACAATGT TTGCTTTGAA 
AAAGGTAATA TAAAAGTAGA CTTTACATTT ATGAGGGCCA AATGTTTTTT GGTGAAAGGG 
CAAAAATATA ATGATTTGTC TTTTCAAGGT AAAAGCCTTA GAGTTCCTGA GAAAGTACCT 
TTTCGAATGA CACAAAACAT TGAAACAGCA CTGGGTGTAA CTGGAGTAGA AGGTGTATTT 
AGGCTTTCAT GTGAGCAGGT TTTACACATT ATGCGGCGTG GCAGAGAGAC CCTGCTGACG 
CTGCTGGAGG CCTTTGTGTA CGACCCTCTG GTGGACTGGA CAGCAGGAGG CGAGGCTGGG 
TTTGCTGGTG CTGTCTATGG TGGAGGTGGC CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA 
GAGATGGAGC GAGAGATCAC CCGCAGCCTG TTTTCTTCTA GAGTAGCTGA GATTAAGGTG 
AACTGGTTTA AGAATAGAGA TGAGATGCTG GTTGTGCTTC CCAAGTTGGA CGGTAGCTTA 
GATGAATACC TAAGCTTGCA AGAGCAACTG ACAGATGTGG AAAAACTGCA GGGCAAACTA 
CTGGAGGAAA TAGAGTTTCT AGAAGGAGCT GAAGGGGTGG ATCATCCTTC TCATACTCTG 
CAACACAGGT ATTCTGAGCA CACCCAACTA CAGACTCAGC AAAGAGCTGT TCAGGAAGCA 
ATCCAGGTGA AGCTGAATGA ATTTGAACAA TGGATAACAC ATTATCAGGC TGCATTCAAT 
AATTTAGAAG CAACACAGCT TGCAAGCTTG CTTCAAGAGA TAAGCACACA AATGGACCTT 
GGTCCTCCAA GTTACGTGCC AGCAACAGCC TTTCTGCAGA ATGCTGGTCA GGCCCACTTG 
ATTAGCCAGT GCGAGCAGCT GGAGGGGGAG GTTGGTGCTC TCCTGCAGCA GAGGCGCTCC 
GTGCTCCGTG GCTGTCTGGA GCAACTGCAT CACTATGCAA CCGTGGCCCT GCAGTATCCG 
AAGGCCATAT TTCAGAAACA TCGAATTGAA CAGTGGAAGA CCTGGATGGA AGAGCTCATC 
TGTAACACCA CAGTAGAGCG TTGTCAAGAG CTCTATAGGA AATATGAAAT GCAATATGCT 
CCCCAGCCAC CCCCAACAGT GTGTCAGTTC ATCACTGCCA CTGAAATGAC CCTGCAGCGA 
TACGCAGCAG ACATCAACAG CAGACTTATT AGACAAGTGG AACGCTTGAA ACAGGAAGCT 
GTCACTGTGC CAGTTTGTGA AGATCAGTTG AAAGAAATTG AACGTTGCAT TAAAGTTTTC 
CTTCATGAGA ATGGAGAAGA AGGATCTTTG AGTCTAGCAA GTGTTATTAT TTCTGCCCTT 
TGTACCCTTA CAAGGCGTAA CCTGATGATG GAAGGTGCAG CGTCAAGTGC TGGAGAACAG 
CTGGTTGATC TGACTTCTCG GGATGGAGCC TGGTTCTTGG AGGAACTCTG CAGTATGAGC 
GGAAACGTCA CCTGCTTGGT TCAGTTACTG AAGCAGTGCC ACCTGGTGCC ACAGGACTTA 
GATATCCCGA ACCCCATGGA AGCGTCTGAG ACAGTTCACT TAGCCAATGG AGTGTATACC 
TCACTTCAGG AATTGAATTC GAATTTCCGG CAAATCATAT TTCCAGAAGC ACTTCGATGT 
TTAATGAAAG GGGAATACAC GTTAGAAAGT ATGCTGCATG AACTGGACGG TCTTATTGAG 
CAGACCACCG ATGGCGTTCC CCTGCAGACT CTAGTGGAAT CTCTTCAGGC CTACTTAAGA 
AACGCAGCTA TGGGACTGGA AGAAGAAACA CATGCTCATT ACATCGATGT TGCCAGACTA 
CTACATGCTC AGTACGGTGA ATTAATCCAA CCGAGAAATG GTTCAGTTGA TGAAACACCC 
AAAATGTCAG CTGGCCAGAT GCTTTTGGTA GCATTCGATG GCATGTTTGC TCAAGTTGAA 
ACTGCTTTCA GCTTATTAGT TGAAAAGTTG AACAAGATGG AAATTCCCAT AGCTTGGCGA 
AAGATTGACA TCATAAGGGA AGCCAGGAGT ACTCAAGTTA ATTTTTTTGA TGATGATAAT 
CACCGGCAGG TGCTAGAAGA GATTTTCTTT CTAAAAAGAC TACAGACTAT TAAGGAGTTC 
TTCAGGCTCT GTGGTACCTT TTCTAAAACA TTGTCAGGAT CAAGTTCACT TGAAGATCAG 
AATACTGTGA ATGGGCCTGT ACAGATTGTC AATGTGAAAA CCCTTTTTAG AAACTCTTGT 
TTCAGTGAAG ACCAAATGGC CAAACCTATC AAGGCATTCA CAGCTGACTT TGTGAGGCAG 
CTCTTGATAG GGCTACCCAA CCAAGCCCTC GGACTCACAC TGTGCAGTTT TATCAGTGCT 
CTGGGTGTAG ACATCATTGC TCAAGTAGAG GCAAAGGACT TTGGTGCCGA AAGCAAAGTT 
TCTGTTGATG ATCTCTGTAA GAAAGCGGTG GAACATAACA TCCAGATAGG GAAGTTCTCT 
CAGCTGGTTA TGAACAGGGC AACTGTGTTA GCAAGTTCTT ACGACACTGC CTGGAAGAAG 
CATGACTTGG TGCGAAGGCT AGAAACCAGT ATTTCTTCTT GTAAGACAAG CCTGCAGCGG 
GTTCAGCTGC ATATTGCCAT GTTTCAGTGG CAACATGAAG ATCTACTTAT CAATAGACCA 
CAAGCCATGT CAGTCACACC TCCCCCACGG TCTGCTATCC TAACCAGCAT GAAAAAGAAG 
CTGCATACCC TGAGCCAGAT TGAAACTTCT ATTGCAACAG TTCAGGAGAA GCTAGCTGCA 
CTTGAATCAA GTATTGAACA GCGACTCAAG TGGGCAGGTG GTGCCAACCC TGCATTGGCC 
CCTGTACTAC AAGATTTTGA AGCAACGATA GCTGAAAGAA GAAATCTTGT CCTTAAAGAG 
AGCCAAAGAG CAAGTCAGGT CACATTTCTC TGCAGCAATA TCATTCATTT TGAAAGTTTA 
CGAACAAGAA CTGCAGAAGC CTTAAACCTG GATGCGGCGT TATTTGAACT AATCAAGCGA 
TGTCAGCAGA TGTGTTCGTT TGCATCACAG TTTAACAGTT CAGTGTCTGA GTTAGAGCTT 
CGTTTATTAC AGAGAGTGGA CACTGGTCTT GAACATCCTA TTGGCAGCTC TGAATGGCTT 
TTGTCAGCAC ACAAACAGTT GACCCAGGAT ATGTCTACTC AGAGGGCAAT TCAGACAGAG 
AAAGAGCAGC AGATAGAAAC GGTCTGTGAA ACAATTCAGA ATCTGGTTGA TAATATAAAG 
ACTGTGCTCA CTGGTCATAA CCGACAGCTT GGAGATGTCA AACATCTCTT GAAAGCTATG 
GCTAAGGATG AAGAAGCTGC TCTGGCAGAT GGTGAAGATG TTCCCTATGA GAACAGTGTT 
AGGCAGTTTT TGGGTGAATA TAAATCATGG CAAGACAACA TTCAAACAGT TCTATTTACA 
TTAGTCCAGG CTATGGGTCA GGTTCGAAGT CAAGAACACG TTGAAATGCT CCAGGAAATC 
ACTCCCACCT TGAAAGAACT GAAAACACAA AGTCAGAGTA TCTATAATAA TTTAGTGAGT 
TTTGCATCAC CCTTAGTCAC CGATGCAACA AATGAATGTT CGAGTCCAAC GTCATCTGCT 
ACTTATCAGC CATCCTTCGC TGCAGCAGTC CGGAGTAACA CTGGCCAGAA GACTCAGCCT 
GATGTCATGT CACAGAATGC TAGAAAGCTG ATCCAAAAAA ATCTTGCTAC ATCAGCTGAT 
ACTCCACCAA GCACCGTTCC AGGAACTGGC AAGAGTGTTG CTTGTAGTCC TAAAAAGGCA 
GTCAGAGACC CTAAAACTGG GAAAGCGGTG CAAGAGAGAA ACTCCTATGC AGTGAGTGTG 
TGGAAGAGAG TGAAAGCCAA GTTAGAGGGC CGAGATGTTG ATCCGAATAG GAGGATGTCA 
GTTGCTGAAC AGGTTGACTA TGTCATTAAG GAAGCAACTA ATCTAGATAA CTTGGCTCAG 
CTGTATGAAG GTTGGACAGC CTGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_017023066.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878555.1 Homo sapiens SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X4, mRNA.

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CloneID	OHu49089
Clone ID Related Accession (Same CDS sequence)	XM_017023066.1 , XM_005255183.4 , XM_005255183.3 , XM_017023066.2
Accession Version	XM_017023066.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATAATTTAAA ATATTCTTCA 
TCCAGAGATA GAGGTGGTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACGCTGACA TACAGAATGA CGAAAAGGGT 
GGCTACAGTG TCAATGGAGG ATCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAACAATGT GACCAGCCCT GAGTTCACCA GTGTTCAGCA TGGCAGTCGT 
GCTTTAGCCA CCAAAGACAT GAGGAAATCA CAGGAGAGAT CGATGTCTTA TTCTGATGAG 
TCTCGACTGT CGAATCTTCT TCGGAGGATC ACCCGGGAAG ACGACAGAGA CCGAAGATTG 
GCTACTGTAA AGCAGTTGAA AGAATTTATT CAGCAACCAG AAAATAAGCT GGTACTAGTT 
AAACAATTGG ATAATATCTT GGCTGCTGTA CATGACGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAGT TGAGACAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTCTGAGC 
TATGAGGCTG AGAAGATCTT CAAGTGGATT TTTAGCAAAT TTAGCTCATC TGCAAAAGAT 
GAAGTTAAAC TCCTCTACTT ATGTGCCACC TACAAAGCAC TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCTGTAAT GCAGCTTGTA ATGACCAGCC TGCAGTCTAT TCTTGAAAAT 
GTGGATACAC CAGAATTGCT TTGTAAATGT GTTAAGTGCA TTCTTTTGGT GGCTCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACAGTTG ATATATTAGT TGGATGGCAT 
ATAGATCATA CTCAGAAACC TTCGCTCACG CAGCAGGTAT CTGGGTGGTT GCAGAGTTTG 
GAGCCATTTT GGGTAGCTGA TCTTGCATTT TCTACTACTC TTCTTGGTCA GTTTCTGGAA 
GACATGGAAG CATATGCTGA GGACCTCAGC CATGTGGCCT CTGGGGAATC AGTGGATGAA 
GATGTCCCTC CTCCATCAGT GTCATTACCA AAGCTGGCTG CACTTCTCCG GGTATTTAGT 
ACTGTGGTGA GGAGCATTGG GGAACGCTTC AGCCCAATTC GGGGTCCTCC AATTACTGAG 
GCATATGTAA CAGATGTTCT GTACAGAGTA ATGAGATGTG TGACGGCTGC AAACCAGGTG 
TTTTTTTCTG AGGCTGTGTT GACAGCTGCT AATGAGTGTG TTGGTGTTTT GCTCGGCAGC 
TTGGATCCTA GCATGACTAT ACATTGTGAC ATGGTCATTA CATATGGATT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACCGATTAT ATCATCTCAG TCTTGAATTT ACTCACGCTG 
ATTGTTGAAC AGATAAATAC GAAACTGCCA TCATCATTTG TAGAAAAACT GTTTATACCA 
TCATCTAAAC TACTATTCTT GCGTTATCAT AAAGAAAAAG AGGTTGTTGC TGTAGCCCAT 
GCTGTTTATC AAGCAGTGCT CAGCTTGAAG AATATTCCTG TTTTGGAGAC TGCCTATAAG 
TTAATATTGG GAGAAATGAC TTGTGCCCTA AACAACCTCC TACACAGTCT ACAACTTCCT 
GAGGCCTGTT CTGAAATAAA ACATGAGGCT TTTAAGAATC ATGTGTTCAA TGTAGACAAT 
GCAAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACTA CAATTGGAAA TGCCAAAAAC 
TCACTAATAG GGATGTGGGC GCTATCTCCA ACTGTCTTTG CACTTCTGAG TAAGAATCTG 
ATGATTGTGC ACAGTGACCT GGCTGTTCAC TTCCCTGCCA TTCAGTATGC TGTGCTCTAC 
ACATTGTATT CTCATTGTAC CAGGCATGAT CACTTTATCT CTAGTAGCCT CAGTTCTTCC 
TCTCCTTCTT TGTTTGATGG AGCTGTGATT AGCACTGTAA CTACGGCTAC AAAGAAACAT 
TTCTCAATTA TATTAAATCT TCTGGGAATA TTACTTAAGA AAGATAACCT TAACCAGGAC 
ACGAGGAAAC TGTTAATGAC TTGGGCTTTG GAAGCAGCTG TTTTAATGAA GAAGTCTGAA 
ACATACGCAC CTTTATTCTC TCTTCCGTCT TTCCATAAAT TTTGCAAAGG CCTTTTAGCC 
AACACTCTCG TTGAAGATGT GAATATCTGT CTGCAGGCAT GCAGCAGTCT ACATGCTCTG 
TCCTCTTCCT TGCCAGATGA TCTTTTACAG AGATGTGTCG ATGTTTGCCG TGTTCAACTA 
GTGCACAGTG GAACTCGTAT TCGACAAGCA TTTGGAAAAC TGTTGAAATC AATTCCTTTA 
GATGTTGTCC TAAGCAATAA CAATCACACA GAAATTCAAG AAATTTCTTT AGCATTAAGA 
AGTCACATGA GTAAAGCACC AAGTAATACA TTCCACCCCC AAGATTTCTC TGATGTTATT 
AGTTTTATTT TGTATGGGAA CTCTCATAGA ACAGGGAAGG ACAATTGGTT GGAAAGACTG 
TTCTATAGCT GCCAGAGACT GGATAAGCGT GACCAGTCAA CAATTCCACG CAATCTCCTG 
AAGACAGATG CTGTCCTTTG GCAGTGGGCC ATATGGGAAG CTGCACAATT CACTGTTCTT 
TCTAAGCTGA GAACCCCACT GGGCAGAGCT CAAGACACCT TCCAGACAAT TGAAGGTATC 
ATTCGAAGTC TCGCAGCTCA CACATTAAAC CCTGATCAGG ATGTTAGTCA GTGGACAACT 
GCAGACAATG ATGAAGGCCA TGGTAACAAC CAACTTAGAC TTGTTCTTCT TCTGCAGTAT 
CTGGAAAATC TGGAGAAATT AATGTATAAT GCATACGAGG GATGTGCTAA TGCATTAACT 
TCACCTCCCA AGGTCATTAG AACTTTTTTC TATACCAATC GCCAAACTTG TCAGGACTGG 
CTAACGCGGA TTCGACTCTC CATCATGAGG GTAGGATTGT TGGCAGGCCA GCCTGCAGTG 
ACAGTGAGAC ATGGCTTTGA CTTGCTTACA GAGATGAAAA CAACCAGCCT ATCTCAGGGG 
AATGAATTGG AAGTAACCAT TATGATGGTG GTAGAAGCAT TATGTGAACT TCATTGTCCT 
GAAGCTATAC AGGGAATTGC TGTCTGGTCA TCATCTATTG TTGGAAAAAA TCTTCTGTGG 
ATTAACTCAG TGGCTCAACA GGCTGAAGGG AGGTTTGAAA AGGCCTCTGT GGAGTACCAG 
GAACACCTGT GTGCCATGAC AGGTGTTGAT TGCTGCATCT CCAGCTTTGA CAAATCGGTG 
CTCACCTTAG CCAATGCTGG GCGTAACAGT GCCAGCCCGA AACATTCTCT GAATGGTGAA 
TCCAGAAAAA CTGTGCTGTC CAAACCGACT GACTCTTCCC CTGAGGTTAT AAATTATTTA 
GGAAATAAAG CATGTGAGTG CTACATCTCA ATTGCCGATT GGGCTGCTGT GCAGGAATGG 
CAGAACGCTA TCCATGACTT GAAAAAGAGT ACCAGTAGCA CTTCCCTCAA CCTGAAAGCT 
GACTTCAACT ATATAAAATC ATTAAGCAGC TTTGAGTCTG GAAAATTTGT TGAATGTACC 
GAGCAGTTAG AATTGTTACC AGGAGAAAAT ATCAATCTAC TTGCTGGAGG ATCAAAAGAA 
AAAATAGACA TGAAAAAACT GCTTCCTAAC ATGTTAAGTC CGGATCCGAG GGAACTTCAG 
AAATCCATTG AAGTTCAATT GTTAAGAAGT TCTGTTTGTT TGGCAACTGC TTTAAACCCG 
ATAGAACAAG ATCAGAAGTG GCAGTCTATA ACTGAAAATG TGGTAAAGTA CTTGAAGCAA 
ACATCCCGCA TCGCTATTGG ACCTCTGAGA CTTTCTACTT TAACAGTTTC ACAGTCTTTG 
CCAGTTCTAA GTACCTTGCA GCTGTATTGC TCATCTGCTT TGGAGAACAC AGTTTCTAAC 
AGACTTTCAA CAGAGGACTG TCTTATTCCA CTCTTCAGTG AAGCTTTACG TTCATGTAAA 
CAGCATGACG TGAGGCCATG GATGCAGGCA TTAAGGTATA CTATGTACCA GAATCAGTTG 
TTGGAGAAAA TTAAAGAACA AACAGTCCCA ATTAGAAGCC ATCTCATGGA ATTAGGTCTA 
ACAGCAGCAA AATTTGCTAG AAAACGAGGG AATGTGTCCC TTGCAACAAG ACTGCTGGCA 
CAGTGCAGTG AAGTTCAGCT GGGAAAGACC ACCACTGCAC AGGATTTAGT CCAACATTTT 
AAAAAACTAT CAACCCAAGG TCAAGTGGAT GAAAAATGGG GGCCCGAACT TGATATTGAA 
AAAACCAAAT TGCTTTATAC AGCAGGCCAG TCAACACATG CAATGGAAAT GTTGAGTTCT 
TGTGCCATAT CTTTCTGCAA GTCTGTGAAA GCTGAATATG CAGTTGCTAA ATCAATTCTG 
ACACTGGCTA AATGGATCCA GGCAGAATGG AAAGAGATTT CAGGACAGCT GAAACAGGTT 
TACAGAGCTC AGCACCAACA GAACTTCACA GGTCTTTCTA CTTTGTCTAA AAACATACTC 
ACTCTAATAG AACTGCCATC TGTTAATACG ATGGAAGAAG AGTATCCTCG GATCGAGAGT 
GAATCTACAG TGCATATTGG AGTTGGAGAA CCTGACTTCA TTTTGGGACA GTTGTATCAC 
CTGTCTTCAG TACAGGCACC TGAAGTAGCC AAATCTTGGG CAGCGTTGGC CAGCTGGGCT 
TATAGGTGGG GCAGAAAGGT GGTTGACAAT GCCAGTCAGG GAGAAGGTGT TCGTCTGCTG 
CCTAGAGAAA AATCTGAAGT TCAGAATCTA CTTCCAGACA CTATAACTGA GGAAGAGAAA 
GAGAGAATAT ATGGTATTCT TGGACAGGCT GTGTGTCGGC CGGCGGGGAT TCAGGATGAA 
GATATAACAC TTCAGATAAC TGAGAGTGAA GACAACGAAG AAGATGACAT GGTTGATGTT 
ATCTGGCGTC AGTTGATATC AAGCTGCCCA TGGCTTTCAG AACTTGATGA AAGTGCAACT 
GAAGGAGTTA TTAAAGTGTG GAGGAAAGTT GTAGATAGAA TATTCAGCCT GTACAAACTC 
TCTTGCAGTG CATACTTTAC TTTCCTTAAA CTCAACGCTG GTCAAATTCC TTTAGATGAG 
GATGACCCTA GGCTGCATTT AAGTCACAGA GTGGAACAGA GCACTGATGA CATGATTGTG 
ATGGCCACAT TGCGCCTGCT GCGGTTGCTC GTGAAGCATG CTGGTGAGCT TCGGCAGTAT 
CTGGAGCACG GCTTGGAGAC AACACCCACT GCACCATGGA GAGGAATTAT TCCGCAACTT 
TTCTCACGCT TAAACCACCC TGAAGTGTAT GTGCGCCAAA GTATTTGTAA CCTTCTCTGC 
CGTGTGGCTC AAGATTCCCC ACATCTCATA TTGTATCCTG CAATAGTGGG TACCATATCG 
CTTAGTAGTG AATCCCAGGC TTCAGGAAAT AAATTTTCCA CTGCAATTCC AACTTTACTT 
GGCAATATTC AAGGAGAAGA ATTGCTGGTT TCTGAATGTG AGGGAGGAAG TCCTCCTGCA 
TCTCAGGATA GCAATAAGGA TGAACCTAAA AGTGGATTAA ATGAAGACCA AGCCATGATG 
CAGGATTGTT ACAGCAAAAT TGTAGATAAG CTGTCCTCTG CAAACCCCAC CATGGTATTA 
CAGGTTCAGA TGCTCGTGGC TGAACTGCGC AGGGTCACTG TGCTCTGGGA TGAGCTCTGG 
CTGGGAGTTT TGCTGCAACA ACACATGTAT GTCCTGAGAC GAATTCAGCA GCTTGAAGAT 
GAGGTGAAGA GAGTCCAGAA CAACAACACC TTACGCAAAG AAGAGAAAAT TGCAATCATG 
AGGGAGAAGC ACACAGCTTT GATGAAGCCC ATCGTATTTG CTTTGGAGCA TGTGAGGAGT 
ATCACAGCGG CTCCTGCAGA AACACCTCAT GAAAAATGGT TTCAGGATAA CTATGGTGAT 
GCCATTGAAA ATGCCCTAGA AAAACTGAAG ACTCCATTGA ACCCTGCAAA GCCTGGGAGC 
AGCTGGATTC CATTTAAAGA GATAATGCTA AGTTTGCAAC AGAGAGCACA GAAACGTGCA 
AGTTACATCT TGCGTCTTGA AGAAATCAGT CCATGGTTGG CTGCCATGAC TAACACTGAA 
ATTGCTCTTC CTGGGGAAGT CTCAGCCAGA GACACTGTCA CAATCCATAG TGTGGGCGGA 
ACCATCACAA TCTTACCGAC TAAAACCAAG CCAAAGAAAC TTCTCTTTCT TGGATCAGAT 
GGGAAGAGCT ATCCTTATCT TTTCAAAGGA CTGGAGGATT TACATCTGGA TGAGAGAATA 
ATGCAGTTCC TATCTATTGT GAATACCATG TTTGCTACAA TTAATCGCCA AGAAACACCC 
CGGTTCCATG CTCGACACTA TTCTGTAACA CCACTAGGAA CAAGATCAGG ACTAATCCAG 
TGGGTAGATG GAGCCACACC CTTATTTGGT CTTTACAAAC GATGGCAACA ACGGGAAGCT 
GCCTTACAAG CACAAAAGGC CCAAGATTCC TACCAAACTC CTCAGAATCC TGGAATTGTA 
CCCCGTCCTA GTGAACTTTA TTACAGTAAA ATTGGCCCTG CTTTGAAAAC AGTTGGGCTT 
AGCCTGGATG TGTCCCGTCG GGATTGGCCT CTTCATGTAA TGAAGGCAGT ATTGGAAGAG 
TTAATGGAGG CCACACCCCC GAATCTCCTT GCCAAAGAGC TCTGGTCATC TTGCACAACA 
CCTGATGAAT GGTGGAGAGT TACGCAGTCT TATGCAAGAT CTACTGCAGT CATGTCTATG 
GTTGGATACA TAATTGGCCT TGGAGACAGA CATCTGGATA ATGTTCTTAT AGATATGACG 
ACTGGAGAAG TTGTTCACAT AGATTACAAT GTTTGCTTTG AAAAAGGTAA TATAAAAGTA 
GACTTTACAT TTATGAGGGC CAAATGTTTT TTGGTGAAAG GGCAAAAATA TAATGATTTG 
TCTTTTCAAG GTAAAAGCCT TAGAGTTCCT GAGAAAGTAC CTTTTCGAAT GACACAAAAC 
ATTGAAACAG CACTGGGTGT AACTGGAGTA GAAGGTGTAT TTAGGCTTTC ATGTGAGCAG 
GTTTTACACA TTATGCGGCG TGGCAGAGAG ACCCTGCTGA CGCTGCTGGA GGCCTTTGTG 
TACGACCCTC TGGTGGACTG GACAGCAGGA GGCGAGGCTG GGTTTGCTGG TGCTGTCTAT 
GGTGGAGGTG GCCAGCAGGC CGAGAGCAAG CAGAGCAAGA GAGAGATGGA GCGAGAGATC 
ACCCGCAGCC TGTTTTCTTC TAGAGTAGCT GAGATTAAGG TGAACTGGTT TAAGAATAGA 
GATGAGATGC TGGTTGTGCT TCCCAAGTTG GACGGTAGCT TAGATGAATA CCTAAGCTTG 
CAAGAGCAAC TGACAGATGT GGAAAAACTG CAGGGCAAAC TACTGGAGGA AATAGAGTTT 
CTAGAAGGAG CTGAAGGGGT GGATCATCCT TCTCATACTC TGCAACACAG GTATTCTGAG 
CACACCCAAC TACAGACTCA GCAAAGAGCT GTTCAGGAAG CAATCCAGGT GAAGCTGAAT 
GAATTTGAAC AATGGATAAC ACATTATCAG GCTGCATTCA ATAATTTAGA AGCAACACAG 
CTTGCAAGCT TGCTTCAAGA GATAAGCACA CAAATGGACC TTGGTCCTCC AAGTTACGTG 
CCAGCAACAG CCTTTCTGCA GAATGCTGGT CAGGCCCACT TGATTAGCCA GTGCGAGCAG 
CTGGAGGGGG AGGTTGGTGC TCTCCTGCAG CAGAGGCGCT CCGTGCTCCG TGGCTGTCTG 
GAGCAACTGC ATCACTATGC AACCGTGGCC CTGCAGTATC CGAAGGCCAT ATTTCAGAAA 
CATCGAATTG AACAGTGGAA GACCTGGATG GAAGAGCTCA TCTGTAACAC CACAGTAGAG 
CGTTGTCAAG AGCTCTATAG GAAATATGAA ATGCAATATG CTCCCCAGCC ACCCCCAACA 
GTGTGTCAGT TCATCACTGC CACTGAAATG ACCCTGCAGC GATACGCAGC AGACATCAAC 
AGCAGACTTA TTAGACAAGT GGAACGCTTG AAACAGGAAG CTGTCACTGT GCCAGTTTGT 
GAAGATCAGT TGAAAGAAAT TGAACGTTGC ATTAAAGTTT TCCTTCATGA GAATGGAGAA 
GAAGGATCTT TGAGTCTAGC AAGTGTTATT ATTTCTGCCC TTTGTACCCT TACAAGGCGT 
AACCTGATGA TGGAAGGTGC AGCGTCAAGT GCTGGAGAAC AGCTGGTTGA TCTGACTTCT 
CGGGATGGAG CCTGGTTCTT GGAGGAACTC TGCAGTATGA GCGGAAACGT CACCTGCTTG 
GTTCAGTTAC TGAAGCAGTG CCACCTGGTG CCACAGGACT TAGATATCCC GAACCCCATG 
GAAGCGTCTG AGACAGTTCA CTTAGCCAAT GGAGTGTATA CCTCACTTCA GGAATTGAAT 
TCGAATTTCC GGCAAATCAT ATTTCCAGAA GCACTTCGAT GTTTAATGAA AGGGGAATAC 
ACGTTAGAAA GTATGCTGCA TGAACTGGAC GGTCTTATTG AGCAGACCAC CGATGGCGTT 
CCCCTGCAGA CTCTAGTGGA ATCTCTTCAG GCCTACTTAA GAAACGCAGC TATGGGACTG 
GAAGAAGAAA CACATGCTCA TTACATCGAT GTTGCCAGAC TACTACATGC TCAGTACGGT 
GAATTAATCC AACCGAGAAA TGGTTCAGTT GATGAAACAC CCAAAATGTC AGCTGGCCAG 
ATGCTTTTGG TAGCATTCGA TGGCATGTTT GCTCAAGTTG AAACTGCTTT CAGCTTATTA 
GTTGAAAAGT TGAACAAGAT GGAAATTCCC ATAGCTTGGC GAAAGATTGA CATCATAAGG 
GAAGCCAGGA GTACTCAAGT TAATTTTTTT GATGATGATA ATCACCGGCA GGTGCTAGAA 
GAGATTTTCT TTCTAAAAAG ACTACAGACT ATTAAGGAGT TCTTCAGGCT CTGTGGTACC 
TTTTCTAAAA CATTGTCAGG ATCAAGTTCA CTTGAAGATC AGAATACTGT GAATGGGCCT 
GTACAGATTG TCAATGTGAA AACCCTTTTT AGAAACTCTT GTTTCAGTGA AGACCAAATG 
GCCAAACCTA TCAAGGCATT CACAGCTGAC TTTGTGAGGC AGCTCTTGAT AGGGCTACCC 
AACCAAGCCC TCGGACTCAC ACTGTGCAGT TTTATCAGTG CTCTGGGTGT AGACATCATT 
GCTCAAGTAG AGGCAAAGGA CTTTGGTGCC GAAAGCAAAG TTTCTGTTGA TGATCTCTGT 
AAGAAAGCGG TGGAACATAA CATCCAGATA GGGAAGTTCT CTCAGCTGGT TATGAACAGG 
GCAACTGTGT TAGCAAGTTC TTACGACACT GCCTGGAAGA AGCATGACTT GGTGCGAAGG 
CTAGAAACCA GTATTTCTTC TTGTAAGACA AGCCTGCAGC GGGTTCAGCT GCATATTGCC 
ATGTTTCAGT GGCAACATGA AGATCTACTT ATCAATAGAC CACAAGCCAT GTCAGTCACA 
CCTCCCCCAC GGTCTGCTAT CCTAACCAGC ATGAAAAAGA AGCTGCATAC CCTGAGCCAG 
ATTGAAACTT CTATTGCAAC AGTTCAGGAG AAGCTAGCTG CACTTGAATC AAGTATTGAA 
CAGCGACTCA AGTGGGCAGG TGGTGCCAAC CCTGCATTGG CCCCTGTACT ACAAGATTTT 
GAAGCAACGA TAGCTGAAAG AAGAAATCTT GTCCTTAAAG AGAGCCAAAG AGCAAGTCAG 
GTCACATTTC TCTGCAGCAA TATCATTCAT TTTGAAAGTT TACGAACAAG AACTGCAGAA 
GCCTTAAACC TGGATGCGGC GTTATTTGAA CTAATCAAGC GATGTCAGCA GATGTGTTCG 
TTTGCATCAC AGTTTAACAG TTCAGTGTCT GAGTTAGAGC TTCGTTTATT ACAGAGAGTG 
GACACTGGTC TTGAACATCC TATTGGCAGC TCTGAATGGC TTTTGTCAGC ACACAAACAG 
TTGACCCAGG ATATGTCTAC TCAGAGGGCA ATTCAGACAG AGAAAGAGCA GCAGATAGAA 
ACGGTCTGTG AAACAATTCA GAATCTGGTT GATAATATAA AGACTGTGCT CACTGGTCAT 
AACCGACAGC TTGGAGATGT CAAACATCTC TTGAAAGCTA TGGCTAAGGA TGAAGAAGCT 
GCTCTGGCAG ATGGTGAAGA TGTTCCCTAT GAGAACAGTG TTAGGCAGTT TTTGGGTGAA 
TATAAATCAT GGCAAGACAA CATTCAAACA GTTCTATTTA CATTAGTCCA GGCTATGGGT 
CAGGTTCGAA GTCAAGAACA CGTTGAAATG CTCCAGGAAA TCACTCCCAC CTTGAAAGAA 
CTGAAAACAC AAAGTCAGAG TATCTATAAT AATTTAGTGA GTTTTGCATC ACCCTTAGTC 
ACCGATGCAA CAAATGAATG TTCGAGTCCA ACGTCATCTG CTACTTATCA GCCATCCTTC 
GCTGCAGCAG TCCGGAGTAA CACTGGCCAG AAGACTCAGC CTGATGTCAT GTCACAGAAT 
GCTAGAAAGC TGATCCAAAA AAATCTTGCT ACATCAGCTG ATACTCCACC AAGCACCGTT 
CCAGGAACTG GCAAGAGTGT TGCTTGTAGT CCTAAAAAGG CAGTCAGAGA CCCTAAAACT 
GGGAAAGCGG TGCAAGAGAG AAACTCCTAT GCAGTGAGTG TGTGGAAGAG AGTGAAAGCC 
AAGTTAGAGG GCCGAGATGT TGATCCGAAT AGGAGGATGT CAGTTGCTGA ACAGGTTGAC 
TATGTCATTA AGGAAGCAAC TAATCTAGAT AACTTGGCTC AGCTGTATGA AGGTTGGACA 
GCCTGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878555.1
CDS	265..11256
Translation	MRFLRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSVQHGSRALATKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGNIKVDFTFMRAKCFLVKGQKYNDLSFQGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_017023066.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023066.1

ORF Insert Sequence:

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATAATTTAAA ATATTCTTCA 
TCCAGAGATA GAGGTGGTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACGCTGACA TACAGAATGA CGAAAAGGGT 
GGCTACAGTG TCAATGGAGG ATCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAACAATGT GACCAGCCCT GAGTTCACCA GTGTTCAGCA TGGCAGTCGT 
GCTTTAGCCA CCAAAGACAT GAGGAAATCA CAGGAGAGAT CGATGTCTTA TTCTGATGAG 
TCTCGACTGT CGAATCTTCT TCGGAGGATC ACCCGGGAAG ACGACAGAGA CCGAAGATTG 
GCTACTGTAA AGCAGTTGAA AGAATTTATT CAGCAACCAG AAAATAAGCT GGTACTAGTT 
AAACAATTGG ATAATATCTT GGCTGCTGTA CATGACGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAGT TGAGACAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTCTGAGC 
TATGAGGCTG AGAAGATCTT CAAGTGGATT TTTAGCAAAT TTAGCTCATC TGCAAAAGAT 
GAAGTTAAAC TCCTCTACTT ATGTGCCACC TACAAAGCAC TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCTGTAAT GCAGCTTGTA ATGACCAGCC TGCAGTCTAT TCTTGAAAAT 
GTGGATACAC CAGAATTGCT TTGTAAATGT GTTAAGTGCA TTCTTTTGGT GGCTCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACAGTTG ATATATTAGT TGGATGGCAT 
ATAGATCATA CTCAGAAACC TTCGCTCACG CAGCAGGTAT CTGGGTGGTT GCAGAGTTTG 
GAGCCATTTT GGGTAGCTGA TCTTGCATTT TCTACTACTC TTCTTGGTCA GTTTCTGGAA 
GACATGGAAG CATATGCTGA GGACCTCAGC CATGTGGCCT CTGGGGAATC AGTGGATGAA 
GATGTCCCTC CTCCATCAGT GTCATTACCA AAGCTGGCTG CACTTCTCCG GGTATTTAGT 
ACTGTGGTGA GGAGCATTGG GGAACGCTTC AGCCCAATTC GGGGTCCTCC AATTACTGAG 
GCATATGTAA CAGATGTTCT GTACAGAGTA ATGAGATGTG TGACGGCTGC AAACCAGGTG 
TTTTTTTCTG AGGCTGTGTT GACAGCTGCT AATGAGTGTG TTGGTGTTTT GCTCGGCAGC 
TTGGATCCTA GCATGACTAT ACATTGTGAC ATGGTCATTA CATATGGATT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACCGATTAT ATCATCTCAG TCTTGAATTT ACTCACGCTG 
ATTGTTGAAC AGATAAATAC GAAACTGCCA TCATCATTTG TAGAAAAACT GTTTATACCA 
TCATCTAAAC TACTATTCTT GCGTTATCAT AAAGAAAAAG AGGTTGTTGC TGTAGCCCAT 
GCTGTTTATC AAGCAGTGCT CAGCTTGAAG AATATTCCTG TTTTGGAGAC TGCCTATAAG 
TTAATATTGG GAGAAATGAC TTGTGCCCTA AACAACCTCC TACACAGTCT ACAACTTCCT 
GAGGCCTGTT CTGAAATAAA ACATGAGGCT TTTAAGAATC ATGTGTTCAA TGTAGACAAT 
GCAAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACTA CAATTGGAAA TGCCAAAAAC 
TCACTAATAG GGATGTGGGC GCTATCTCCA ACTGTCTTTG CACTTCTGAG TAAGAATCTG 
ATGATTGTGC ACAGTGACCT GGCTGTTCAC TTCCCTGCCA TTCAGTATGC TGTGCTCTAC 
ACATTGTATT CTCATTGTAC CAGGCATGAT CACTTTATCT CTAGTAGCCT CAGTTCTTCC 
TCTCCTTCTT TGTTTGATGG AGCTGTGATT AGCACTGTAA CTACGGCTAC AAAGAAACAT 
TTCTCAATTA TATTAAATCT TCTGGGAATA TTACTTAAGA AAGATAACCT TAACCAGGAC 
ACGAGGAAAC TGTTAATGAC TTGGGCTTTG GAAGCAGCTG TTTTAATGAA GAAGTCTGAA 
ACATACGCAC CTTTATTCTC TCTTCCGTCT TTCCATAAAT TTTGCAAAGG CCTTTTAGCC 
AACACTCTCG TTGAAGATGT GAATATCTGT CTGCAGGCAT GCAGCAGTCT ACATGCTCTG 
TCCTCTTCCT TGCCAGATGA TCTTTTACAG AGATGTGTCG ATGTTTGCCG TGTTCAACTA 
GTGCACAGTG GAACTCGTAT TCGACAAGCA TTTGGAAAAC TGTTGAAATC AATTCCTTTA 
GATGTTGTCC TAAGCAATAA CAATCACACA GAAATTCAAG AAATTTCTTT AGCATTAAGA 
AGTCACATGA GTAAAGCACC AAGTAATACA TTCCACCCCC AAGATTTCTC TGATGTTATT 
AGTTTTATTT TGTATGGGAA CTCTCATAGA ACAGGGAAGG ACAATTGGTT GGAAAGACTG 
TTCTATAGCT GCCAGAGACT GGATAAGCGT GACCAGTCAA CAATTCCACG CAATCTCCTG 
AAGACAGATG CTGTCCTTTG GCAGTGGGCC ATATGGGAAG CTGCACAATT CACTGTTCTT 
TCTAAGCTGA GAACCCCACT GGGCAGAGCT CAAGACACCT TCCAGACAAT TGAAGGTATC 
ATTCGAAGTC TCGCAGCTCA CACATTAAAC CCTGATCAGG ATGTTAGTCA GTGGACAACT 
GCAGACAATG ATGAAGGCCA TGGTAACAAC CAACTTAGAC TTGTTCTTCT TCTGCAGTAT 
CTGGAAAATC TGGAGAAATT AATGTATAAT GCATACGAGG GATGTGCTAA TGCATTAACT 
TCACCTCCCA AGGTCATTAG AACTTTTTTC TATACCAATC GCCAAACTTG TCAGGACTGG 
CTAACGCGGA TTCGACTCTC CATCATGAGG GTAGGATTGT TGGCAGGCCA GCCTGCAGTG 
ACAGTGAGAC ATGGCTTTGA CTTGCTTACA GAGATGAAAA CAACCAGCCT ATCTCAGGGG 
AATGAATTGG AAGTAACCAT TATGATGGTG GTAGAAGCAT TATGTGAACT TCATTGTCCT 
GAAGCTATAC AGGGAATTGC TGTCTGGTCA TCATCTATTG TTGGAAAAAA TCTTCTGTGG 
ATTAACTCAG TGGCTCAACA GGCTGAAGGG AGGTTTGAAA AGGCCTCTGT GGAGTACCAG 
GAACACCTGT GTGCCATGAC AGGTGTTGAT TGCTGCATCT CCAGCTTTGA CAAATCGGTG 
CTCACCTTAG CCAATGCTGG GCGTAACAGT GCCAGCCCGA AACATTCTCT GAATGGTGAA 
TCCAGAAAAA CTGTGCTGTC CAAACCGACT GACTCTTCCC CTGAGGTTAT AAATTATTTA 
GGAAATAAAG CATGTGAGTG CTACATCTCA ATTGCCGATT GGGCTGCTGT GCAGGAATGG 
CAGAACGCTA TCCATGACTT GAAAAAGAGT ACCAGTAGCA CTTCCCTCAA CCTGAAAGCT 
GACTTCAACT ATATAAAATC ATTAAGCAGC TTTGAGTCTG GAAAATTTGT TGAATGTACC 
GAGCAGTTAG AATTGTTACC AGGAGAAAAT ATCAATCTAC TTGCTGGAGG ATCAAAAGAA 
AAAATAGACA TGAAAAAACT GCTTCCTAAC ATGTTAAGTC CGGATCCGAG GGAACTTCAG 
AAATCCATTG AAGTTCAATT GTTAAGAAGT TCTGTTTGTT TGGCAACTGC TTTAAACCCG 
ATAGAACAAG ATCAGAAGTG GCAGTCTATA ACTGAAAATG TGGTAAAGTA CTTGAAGCAA 
ACATCCCGCA TCGCTATTGG ACCTCTGAGA CTTTCTACTT TAACAGTTTC ACAGTCTTTG 
CCAGTTCTAA GTACCTTGCA GCTGTATTGC TCATCTGCTT TGGAGAACAC AGTTTCTAAC 
AGACTTTCAA CAGAGGACTG TCTTATTCCA CTCTTCAGTG AAGCTTTACG TTCATGTAAA 
CAGCATGACG TGAGGCCATG GATGCAGGCA TTAAGGTATA CTATGTACCA GAATCAGTTG 
TTGGAGAAAA TTAAAGAACA AACAGTCCCA ATTAGAAGCC ATCTCATGGA ATTAGGTCTA 
ACAGCAGCAA AATTTGCTAG AAAACGAGGG AATGTGTCCC TTGCAACAAG ACTGCTGGCA 
CAGTGCAGTG AAGTTCAGCT GGGAAAGACC ACCACTGCAC AGGATTTAGT CCAACATTTT 
AAAAAACTAT CAACCCAAGG TCAAGTGGAT GAAAAATGGG GGCCCGAACT TGATATTGAA 
AAAACCAAAT TGCTTTATAC AGCAGGCCAG TCAACACATG CAATGGAAAT GTTGAGTTCT 
TGTGCCATAT CTTTCTGCAA GTCTGTGAAA GCTGAATATG CAGTTGCTAA ATCAATTCTG 
ACACTGGCTA AATGGATCCA GGCAGAATGG AAAGAGATTT CAGGACAGCT GAAACAGGTT 
TACAGAGCTC AGCACCAACA GAACTTCACA GGTCTTTCTA CTTTGTCTAA AAACATACTC 
ACTCTAATAG AACTGCCATC TGTTAATACG ATGGAAGAAG AGTATCCTCG GATCGAGAGT 
GAATCTACAG TGCATATTGG AGTTGGAGAA CCTGACTTCA TTTTGGGACA GTTGTATCAC 
CTGTCTTCAG TACAGGCACC TGAAGTAGCC AAATCTTGGG CAGCGTTGGC CAGCTGGGCT 
TATAGGTGGG GCAGAAAGGT GGTTGACAAT GCCAGTCAGG GAGAAGGTGT TCGTCTGCTG 
CCTAGAGAAA AATCTGAAGT TCAGAATCTA CTTCCAGACA CTATAACTGA GGAAGAGAAA 
GAGAGAATAT ATGGTATTCT TGGACAGGCT GTGTGTCGGC CGGCGGGGAT TCAGGATGAA 
GATATAACAC TTCAGATAAC TGAGAGTGAA GACAACGAAG AAGATGACAT GGTTGATGTT 
ATCTGGCGTC AGTTGATATC AAGCTGCCCA TGGCTTTCAG AACTTGATGA AAGTGCAACT 
GAAGGAGTTA TTAAAGTGTG GAGGAAAGTT GTAGATAGAA TATTCAGCCT GTACAAACTC 
TCTTGCAGTG CATACTTTAC TTTCCTTAAA CTCAACGCTG GTCAAATTCC TTTAGATGAG 
GATGACCCTA GGCTGCATTT AAGTCACAGA GTGGAACAGA GCACTGATGA CATGATTGTG 
ATGGCCACAT TGCGCCTGCT GCGGTTGCTC GTGAAGCATG CTGGTGAGCT TCGGCAGTAT 
CTGGAGCACG GCTTGGAGAC AACACCCACT GCACCATGGA GAGGAATTAT TCCGCAACTT 
TTCTCACGCT TAAACCACCC TGAAGTGTAT GTGCGCCAAA GTATTTGTAA CCTTCTCTGC 
CGTGTGGCTC AAGATTCCCC ACATCTCATA TTGTATCCTG CAATAGTGGG TACCATATCG 
CTTAGTAGTG AATCCCAGGC TTCAGGAAAT AAATTTTCCA CTGCAATTCC AACTTTACTT 
GGCAATATTC AAGGAGAAGA ATTGCTGGTT TCTGAATGTG AGGGAGGAAG TCCTCCTGCA 
TCTCAGGATA GCAATAAGGA TGAACCTAAA AGTGGATTAA ATGAAGACCA AGCCATGATG 
CAGGATTGTT ACAGCAAAAT TGTAGATAAG CTGTCCTCTG CAAACCCCAC CATGGTATTA 
CAGGTTCAGA TGCTCGTGGC TGAACTGCGC AGGGTCACTG TGCTCTGGGA TGAGCTCTGG 
CTGGGAGTTT TGCTGCAACA ACACATGTAT GTCCTGAGAC GAATTCAGCA GCTTGAAGAT 
GAGGTGAAGA GAGTCCAGAA CAACAACACC TTACGCAAAG AAGAGAAAAT TGCAATCATG 
AGGGAGAAGC ACACAGCTTT GATGAAGCCC ATCGTATTTG CTTTGGAGCA TGTGAGGAGT 
ATCACAGCGG CTCCTGCAGA AACACCTCAT GAAAAATGGT TTCAGGATAA CTATGGTGAT 
GCCATTGAAA ATGCCCTAGA AAAACTGAAG ACTCCATTGA ACCCTGCAAA GCCTGGGAGC 
AGCTGGATTC CATTTAAAGA GATAATGCTA AGTTTGCAAC AGAGAGCACA GAAACGTGCA 
AGTTACATCT TGCGTCTTGA AGAAATCAGT CCATGGTTGG CTGCCATGAC TAACACTGAA 
ATTGCTCTTC CTGGGGAAGT CTCAGCCAGA GACACTGTCA CAATCCATAG TGTGGGCGGA 
ACCATCACAA TCTTACCGAC TAAAACCAAG CCAAAGAAAC TTCTCTTTCT TGGATCAGAT 
GGGAAGAGCT ATCCTTATCT TTTCAAAGGA CTGGAGGATT TACATCTGGA TGAGAGAATA 
ATGCAGTTCC TATCTATTGT GAATACCATG TTTGCTACAA TTAATCGCCA AGAAACACCC 
CGGTTCCATG CTCGACACTA TTCTGTAACA CCACTAGGAA CAAGATCAGG ACTAATCCAG 
TGGGTAGATG GAGCCACACC CTTATTTGGT CTTTACAAAC GATGGCAACA ACGGGAAGCT 
GCCTTACAAG CACAAAAGGC CCAAGATTCC TACCAAACTC CTCAGAATCC TGGAATTGTA 
CCCCGTCCTA GTGAACTTTA TTACAGTAAA ATTGGCCCTG CTTTGAAAAC AGTTGGGCTT 
AGCCTGGATG TGTCCCGTCG GGATTGGCCT CTTCATGTAA TGAAGGCAGT ATTGGAAGAG 
TTAATGGAGG CCACACCCCC GAATCTCCTT GCCAAAGAGC TCTGGTCATC TTGCACAACA 
CCTGATGAAT GGTGGAGAGT TACGCAGTCT TATGCAAGAT CTACTGCAGT CATGTCTATG 
GTTGGATACA TAATTGGCCT TGGAGACAGA CATCTGGATA ATGTTCTTAT AGATATGACG 
ACTGGAGAAG TTGTTCACAT AGATTACAAT GTTTGCTTTG AAAAAGGTAA TATAAAAGTA 
GACTTTACAT TTATGAGGGC CAAATGTTTT TTGGTGAAAG GGCAAAAATA TAATGATTTG 
TCTTTTCAAG GTAAAAGCCT TAGAGTTCCT GAGAAAGTAC CTTTTCGAAT GACACAAAAC 
ATTGAAACAG CACTGGGTGT AACTGGAGTA GAAGGTGTAT TTAGGCTTTC ATGTGAGCAG 
GTTTTACACA TTATGCGGCG TGGCAGAGAG ACCCTGCTGA CGCTGCTGGA GGCCTTTGTG 
TACGACCCTC TGGTGGACTG GACAGCAGGA GGCGAGGCTG GGTTTGCTGG TGCTGTCTAT 
GGTGGAGGTG GCCAGCAGGC CGAGAGCAAG CAGAGCAAGA GAGAGATGGA GCGAGAGATC 
ACCCGCAGCC TGTTTTCTTC TAGAGTAGCT GAGATTAAGG TGAACTGGTT TAAGAATAGA 
GATGAGATGC TGGTTGTGCT TCCCAAGTTG GACGGTAGCT TAGATGAATA CCTAAGCTTG 
CAAGAGCAAC TGACAGATGT GGAAAAACTG CAGGGCAAAC TACTGGAGGA AATAGAGTTT 
CTAGAAGGAG CTGAAGGGGT GGATCATCCT TCTCATACTC TGCAACACAG GTATTCTGAG 
CACACCCAAC TACAGACTCA GCAAAGAGCT GTTCAGGAAG CAATCCAGGT GAAGCTGAAT 
GAATTTGAAC AATGGATAAC ACATTATCAG GCTGCATTCA ATAATTTAGA AGCAACACAG 
CTTGCAAGCT TGCTTCAAGA GATAAGCACA CAAATGGACC TTGGTCCTCC AAGTTACGTG 
CCAGCAACAG CCTTTCTGCA GAATGCTGGT CAGGCCCACT TGATTAGCCA GTGCGAGCAG 
CTGGAGGGGG AGGTTGGTGC TCTCCTGCAG CAGAGGCGCT CCGTGCTCCG TGGCTGTCTG 
GAGCAACTGC ATCACTATGC AACCGTGGCC CTGCAGTATC CGAAGGCCAT ATTTCAGAAA 
CATCGAATTG AACAGTGGAA GACCTGGATG GAAGAGCTCA TCTGTAACAC CACAGTAGAG 
CGTTGTCAAG AGCTCTATAG GAAATATGAA ATGCAATATG CTCCCCAGCC ACCCCCAACA 
GTGTGTCAGT TCATCACTGC CACTGAAATG ACCCTGCAGC GATACGCAGC AGACATCAAC 
AGCAGACTTA TTAGACAAGT GGAACGCTTG AAACAGGAAG CTGTCACTGT GCCAGTTTGT 
GAAGATCAGT TGAAAGAAAT TGAACGTTGC ATTAAAGTTT TCCTTCATGA GAATGGAGAA 
GAAGGATCTT TGAGTCTAGC AAGTGTTATT ATTTCTGCCC TTTGTACCCT TACAAGGCGT 
AACCTGATGA TGGAAGGTGC AGCGTCAAGT GCTGGAGAAC AGCTGGTTGA TCTGACTTCT 
CGGGATGGAG CCTGGTTCTT GGAGGAACTC TGCAGTATGA GCGGAAACGT CACCTGCTTG 
GTTCAGTTAC TGAAGCAGTG CCACCTGGTG CCACAGGACT TAGATATCCC GAACCCCATG 
GAAGCGTCTG AGACAGTTCA CTTAGCCAAT GGAGTGTATA CCTCACTTCA GGAATTGAAT 
TCGAATTTCC GGCAAATCAT ATTTCCAGAA GCACTTCGAT GTTTAATGAA AGGGGAATAC 
ACGTTAGAAA GTATGCTGCA TGAACTGGAC GGTCTTATTG AGCAGACCAC CGATGGCGTT 
CCCCTGCAGA CTCTAGTGGA ATCTCTTCAG GCCTACTTAA GAAACGCAGC TATGGGACTG 
GAAGAAGAAA CACATGCTCA TTACATCGAT GTTGCCAGAC TACTACATGC TCAGTACGGT 
GAATTAATCC AACCGAGAAA TGGTTCAGTT GATGAAACAC CCAAAATGTC AGCTGGCCAG 
ATGCTTTTGG TAGCATTCGA TGGCATGTTT GCTCAAGTTG AAACTGCTTT CAGCTTATTA 
GTTGAAAAGT TGAACAAGAT GGAAATTCCC ATAGCTTGGC GAAAGATTGA CATCATAAGG 
GAAGCCAGGA GTACTCAAGT TAATTTTTTT GATGATGATA ATCACCGGCA GGTGCTAGAA 
GAGATTTTCT TTCTAAAAAG ACTACAGACT ATTAAGGAGT TCTTCAGGCT CTGTGGTACC 
TTTTCTAAAA CATTGTCAGG ATCAAGTTCA CTTGAAGATC AGAATACTGT GAATGGGCCT 
GTACAGATTG TCAATGTGAA AACCCTTTTT AGAAACTCTT GTTTCAGTGA AGACCAAATG 
GCCAAACCTA TCAAGGCATT CACAGCTGAC TTTGTGAGGC AGCTCTTGAT AGGGCTACCC 
AACCAAGCCC TCGGACTCAC ACTGTGCAGT TTTATCAGTG CTCTGGGTGT AGACATCATT 
GCTCAAGTAG AGGCAAAGGA CTTTGGTGCC GAAAGCAAAG TTTCTGTTGA TGATCTCTGT 
AAGAAAGCGG TGGAACATAA CATCCAGATA GGGAAGTTCT CTCAGCTGGT TATGAACAGG 
GCAACTGTGT TAGCAAGTTC TTACGACACT GCCTGGAAGA AGCATGACTT GGTGCGAAGG 
CTAGAAACCA GTATTTCTTC TTGTAAGACA AGCCTGCAGC GGGTTCAGCT GCATATTGCC 
ATGTTTCAGT GGCAACATGA AGATCTACTT ATCAATAGAC CACAAGCCAT GTCAGTCACA 
CCTCCCCCAC GGTCTGCTAT CCTAACCAGC ATGAAAAAGA AGCTGCATAC CCTGAGCCAG 
ATTGAAACTT CTATTGCAAC AGTTCAGGAG AAGCTAGCTG CACTTGAATC AAGTATTGAA 
CAGCGACTCA AGTGGGCAGG TGGTGCCAAC CCTGCATTGG CCCCTGTACT ACAAGATTTT 
GAAGCAACGA TAGCTGAAAG AAGAAATCTT GTCCTTAAAG AGAGCCAAAG AGCAAGTCAG 
GTCACATTTC TCTGCAGCAA TATCATTCAT TTTGAAAGTT TACGAACAAG AACTGCAGAA 
GCCTTAAACC TGGATGCGGC GTTATTTGAA CTAATCAAGC GATGTCAGCA GATGTGTTCG 
TTTGCATCAC AGTTTAACAG TTCAGTGTCT GAGTTAGAGC TTCGTTTATT ACAGAGAGTG 
GACACTGGTC TTGAACATCC TATTGGCAGC TCTGAATGGC TTTTGTCAGC ACACAAACAG 
TTGACCCAGG ATATGTCTAC TCAGAGGGCA ATTCAGACAG AGAAAGAGCA GCAGATAGAA 
ACGGTCTGTG AAACAATTCA GAATCTGGTT GATAATATAA AGACTGTGCT CACTGGTCAT 
AACCGACAGC TTGGAGATGT CAAACATCTC TTGAAAGCTA TGGCTAAGGA TGAAGAAGCT 
GCTCTGGCAG ATGGTGAAGA TGTTCCCTAT GAGAACAGTG TTAGGCAGTT TTTGGGTGAA 
TATAAATCAT GGCAAGACAA CATTCAAACA GTTCTATTTA CATTAGTCCA GGCTATGGGT 
CAGGTTCGAA GTCAAGAACA CGTTGAAATG CTCCAGGAAA TCACTCCCAC CTTGAAAGAA 
CTGAAAACAC AAAGTCAGAG TATCTATAAT AATTTAGTGA GTTTTGCATC ACCCTTAGTC 
ACCGATGCAA CAAATGAATG TTCGAGTCCA ACGTCATCTG CTACTTATCA GCCATCCTTC 
GCTGCAGCAG TCCGGAGTAA CACTGGCCAG AAGACTCAGC CTGATGTCAT GTCACAGAAT 
GCTAGAAAGC TGATCCAAAA AAATCTTGCT ACATCAGCTG ATACTCCACC AAGCACCGTT 
CCAGGAACTG GCAAGAGTGT TGCTTGTAGT CCTAAAAAGG CAGTCAGAGA CCCTAAAACT 
GGGAAAGCGG TGCAAGAGAG AAACTCCTAT GCAGTGAGTG TGTGGAAGAG AGTGAAAGCC 
AAGTTAGAGG GCCGAGATGT TGATCCGAAT AGGAGGATGT CAGTTGCTGA ACAGGTTGAC 
TATGTCATTA AGGAAGCAAC TAATCTAGAT AACTTGGCTC AGCTGTATGA AGGTTGGACA 
GCCTGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_005255183.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255240.1 Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X3, mRNA.

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CloneID	OHu49089
Clone ID Related Accession (Same CDS sequence)	XM_017023066.1 , XM_005255183.4 , XM_005255183.3 , XM_017023066.2
Accession Version	XM_005255183.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005255183.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATAATTTAAA ATATTCTTCA 
TCCAGAGATA GAGGTGGTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACGCTGACA TACAGAATGA CGAAAAGGGT 
GGCTACAGTG TCAATGGAGG ATCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAACAATGT GACCAGCCCT GAGTTCACCA GTGTTCAGCA TGGCAGTCGT 
GCTTTAGCCA CCAAAGACAT GAGGAAATCA CAGGAGAGAT CGATGTCTTA TTCTGATGAG 
TCTCGACTGT CGAATCTTCT TCGGAGGATC ACCCGGGAAG ACGACAGAGA CCGAAGATTG 
GCTACTGTAA AGCAGTTGAA AGAATTTATT CAGCAACCAG AAAATAAGCT GGTACTAGTT 
AAACAATTGG ATAATATCTT GGCTGCTGTA CATGACGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAGT TGAGACAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTCTGAGC 
TATGAGGCTG AGAAGATCTT CAAGTGGATT TTTAGCAAAT TTAGCTCATC TGCAAAAGAT 
GAAGTTAAAC TCCTCTACTT ATGTGCCACC TACAAAGCAC TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCTGTAAT GCAGCTTGTA ATGACCAGCC TGCAGTCTAT TCTTGAAAAT 
GTGGATACAC CAGAATTGCT TTGTAAATGT GTTAAGTGCA TTCTTTTGGT GGCTCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACAGTTG ATATATTAGT TGGATGGCAT 
ATAGATCATA CTCAGAAACC TTCGCTCACG CAGCAGGTAT CTGGGTGGTT GCAGAGTTTG 
GAGCCATTTT GGGTAGCTGA TCTTGCATTT TCTACTACTC TTCTTGGTCA GTTTCTGGAA 
GACATGGAAG CATATGCTGA GGACCTCAGC CATGTGGCCT CTGGGGAATC AGTGGATGAA 
GATGTCCCTC CTCCATCAGT GTCATTACCA AAGCTGGCTG CACTTCTCCG GGTATTTAGT 
ACTGTGGTGA GGAGCATTGG GGAACGCTTC AGCCCAATTC GGGGTCCTCC AATTACTGAG 
GCATATGTAA CAGATGTTCT GTACAGAGTA ATGAGATGTG TGACGGCTGC AAACCAGGTG 
TTTTTTTCTG AGGCTGTGTT GACAGCTGCT AATGAGTGTG TTGGTGTTTT GCTCGGCAGC 
TTGGATCCTA GCATGACTAT ACATTGTGAC ATGGTCATTA CATATGGATT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACCGATTAT ATCATCTCAG TCTTGAATTT ACTCACGCTG 
ATTGTTGAAC AGATAAATAC GAAACTGCCA TCATCATTTG TAGAAAAACT GTTTATACCA 
TCATCTAAAC TACTATTCTT GCGTTATCAT AAAGAAAAAG AGGTTGTTGC TGTAGCCCAT 
GCTGTTTATC AAGCAGTGCT CAGCTTGAAG AATATTCCTG TTTTGGAGAC TGCCTATAAG 
TTAATATTGG GAGAAATGAC TTGTGCCCTA AACAACCTCC TACACAGTCT ACAACTTCCT 
GAGGCCTGTT CTGAAATAAA ACATGAGGCT TTTAAGAATC ATGTGTTCAA TGTAGACAAT 
GCAAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACTA CAATTGGAAA TGCCAAAAAC 
TCACTAATAG GGATGTGGGC GCTATCTCCA ACTGTCTTTG CACTTCTGAG TAAGAATCTG 
ATGATTGTGC ACAGTGACCT GGCTGTTCAC TTCCCTGCCA TTCAGTATGC TGTGCTCTAC 
ACATTGTATT CTCATTGTAC CAGGCATGAT CACTTTATCT CTAGTAGCCT CAGTTCTTCC 
TCTCCTTCTT TGTTTGATGG AGCTGTGATT AGCACTGTAA CTACGGCTAC AAAGAAACAT 
TTCTCAATTA TATTAAATCT TCTGGGAATA TTACTTAAGA AAGATAACCT TAACCAGGAC 
ACGAGGAAAC TGTTAATGAC TTGGGCTTTG GAAGCAGCTG TTTTAATGAA GAAGTCTGAA 
ACATACGCAC CTTTATTCTC TCTTCCGTCT TTCCATAAAT TTTGCAAAGG CCTTTTAGCC 
AACACTCTCG TTGAAGATGT GAATATCTGT CTGCAGGCAT GCAGCAGTCT ACATGCTCTG 
TCCTCTTCCT TGCCAGATGA TCTTTTACAG AGATGTGTCG ATGTTTGCCG TGTTCAACTA 
GTGCACAGTG GAACTCGTAT TCGACAAGCA TTTGGAAAAC TGTTGAAATC AATTCCTTTA 
GATGTTGTCC TAAGCAATAA CAATCACACA GAAATTCAAG AAATTTCTTT AGCATTAAGA 
AGTCACATGA GTAAAGCACC AAGTAATACA TTCCACCCCC AAGATTTCTC TGATGTTATT 
AGTTTTATTT TGTATGGGAA CTCTCATAGA ACAGGGAAGG ACAATTGGTT GGAAAGACTG 
TTCTATAGCT GCCAGAGACT GGATAAGCGT GACCAGTCAA CAATTCCACG CAATCTCCTG 
AAGACAGATG CTGTCCTTTG GCAGTGGGCC ATATGGGAAG CTGCACAATT CACTGTTCTT 
TCTAAGCTGA GAACCCCACT GGGCAGAGCT CAAGACACCT TCCAGACAAT TGAAGGTATC 
ATTCGAAGTC TCGCAGCTCA CACATTAAAC CCTGATCAGG ATGTTAGTCA GTGGACAACT 
GCAGACAATG ATGAAGGCCA TGGTAACAAC CAACTTAGAC TTGTTCTTCT TCTGCAGTAT 
CTGGAAAATC TGGAGAAATT AATGTATAAT GCATACGAGG GATGTGCTAA TGCATTAACT 
TCACCTCCCA AGGTCATTAG AACTTTTTTC TATACCAATC GCCAAACTTG TCAGGACTGG 
CTAACGCGGA TTCGACTCTC CATCATGAGG GTAGGATTGT TGGCAGGCCA GCCTGCAGTG 
ACAGTGAGAC ATGGCTTTGA CTTGCTTACA GAGATGAAAA CAACCAGCCT ATCTCAGGGG 
AATGAATTGG AAGTAACCAT TATGATGGTG GTAGAAGCAT TATGTGAACT TCATTGTCCT 
GAAGCTATAC AGGGAATTGC TGTCTGGTCA TCATCTATTG TTGGAAAAAA TCTTCTGTGG 
ATTAACTCAG TGGCTCAACA GGCTGAAGGG AGGTTTGAAA AGGCCTCTGT GGAGTACCAG 
GAACACCTGT GTGCCATGAC AGGTGTTGAT TGCTGCATCT CCAGCTTTGA CAAATCGGTG 
CTCACCTTAG CCAATGCTGG GCGTAACAGT GCCAGCCCGA AACATTCTCT GAATGGTGAA 
TCCAGAAAAA CTGTGCTGTC CAAACCGACT GACTCTTCCC CTGAGGTTAT AAATTATTTA 
GGAAATAAAG CATGTGAGTG CTACATCTCA ATTGCCGATT GGGCTGCTGT GCAGGAATGG 
CAGAACGCTA TCCATGACTT GAAAAAGAGT ACCAGTAGCA CTTCCCTCAA CCTGAAAGCT 
GACTTCAACT ATATAAAATC ATTAAGCAGC TTTGAGTCTG GAAAATTTGT TGAATGTACC 
GAGCAGTTAG AATTGTTACC AGGAGAAAAT ATCAATCTAC TTGCTGGAGG ATCAAAAGAA 
AAAATAGACA TGAAAAAACT GCTTCCTAAC ATGTTAAGTC CGGATCCGAG GGAACTTCAG 
AAATCCATTG AAGTTCAATT GTTAAGAAGT TCTGTTTGTT TGGCAACTGC TTTAAACCCG 
ATAGAACAAG ATCAGAAGTG GCAGTCTATA ACTGAAAATG TGGTAAAGTA CTTGAAGCAA 
ACATCCCGCA TCGCTATTGG ACCTCTGAGA CTTTCTACTT TAACAGTTTC ACAGTCTTTG 
CCAGTTCTAA GTACCTTGCA GCTGTATTGC TCATCTGCTT TGGAGAACAC AGTTTCTAAC 
AGACTTTCAA CAGAGGACTG TCTTATTCCA CTCTTCAGTG AAGCTTTACG TTCATGTAAA 
CAGCATGACG TGAGGCCATG GATGCAGGCA TTAAGGTATA CTATGTACCA GAATCAGTTG 
TTGGAGAAAA TTAAAGAACA AACAGTCCCA ATTAGAAGCC ATCTCATGGA ATTAGGTCTA 
ACAGCAGCAA AATTTGCTAG AAAACGAGGG AATGTGTCCC TTGCAACAAG ACTGCTGGCA 
CAGTGCAGTG AAGTTCAGCT GGGAAAGACC ACCACTGCAC AGGATTTAGT CCAACATTTT 
AAAAAACTAT CAACCCAAGG TCAAGTGGAT GAAAAATGGG GGCCCGAACT TGATATTGAA 
AAAACCAAAT TGCTTTATAC AGCAGGCCAG TCAACACATG CAATGGAAAT GTTGAGTTCT 
TGTGCCATAT CTTTCTGCAA GTCTGTGAAA GCTGAATATG CAGTTGCTAA ATCAATTCTG 
ACACTGGCTA AATGGATCCA GGCAGAATGG AAAGAGATTT CAGGACAGCT GAAACAGGTT 
TACAGAGCTC AGCACCAACA GAACTTCACA GGTCTTTCTA CTTTGTCTAA AAACATACTC 
ACTCTAATAG AACTGCCATC TGTTAATACG ATGGAAGAAG AGTATCCTCG GATCGAGAGT 
GAATCTACAG TGCATATTGG AGTTGGAGAA CCTGACTTCA TTTTGGGACA GTTGTATCAC 
CTGTCTTCAG TACAGGCACC TGAAGTAGCC AAATCTTGGG CAGCGTTGGC CAGCTGGGCT 
TATAGGTGGG GCAGAAAGGT GGTTGACAAT GCCAGTCAGG GAGAAGGTGT TCGTCTGCTG 
CCTAGAGAAA AATCTGAAGT TCAGAATCTA CTTCCAGACA CTATAACTGA GGAAGAGAAA 
GAGAGAATAT ATGGTATTCT TGGACAGGCT GTGTGTCGGC CGGCGGGGAT TCAGGATGAA 
GATATAACAC TTCAGATAAC TGAGAGTGAA GACAACGAAG AAGATGACAT GGTTGATGTT 
ATCTGGCGTC AGTTGATATC AAGCTGCCCA TGGCTTTCAG AACTTGATGA AAGTGCAACT 
GAAGGAGTTA TTAAAGTGTG GAGGAAAGTT GTAGATAGAA TATTCAGCCT GTACAAACTC 
TCTTGCAGTG CATACTTTAC TTTCCTTAAA CTCAACGCTG GTCAAATTCC TTTAGATGAG 
GATGACCCTA GGCTGCATTT AAGTCACAGA GTGGAACAGA GCACTGATGA CATGATTGTG 
ATGGCCACAT TGCGCCTGCT GCGGTTGCTC GTGAAGCATG CTGGTGAGCT TCGGCAGTAT 
CTGGAGCACG GCTTGGAGAC AACACCCACT GCACCATGGA GAGGAATTAT TCCGCAACTT 
TTCTCACGCT TAAACCACCC TGAAGTGTAT GTGCGCCAAA GTATTTGTAA CCTTCTCTGC 
CGTGTGGCTC AAGATTCCCC ACATCTCATA TTGTATCCTG CAATAGTGGG TACCATATCG 
CTTAGTAGTG AATCCCAGGC TTCAGGAAAT AAATTTTCCA CTGCAATTCC AACTTTACTT 
GGCAATATTC AAGGAGAAGA ATTGCTGGTT TCTGAATGTG AGGGAGGAAG TCCTCCTGCA 
TCTCAGGATA GCAATAAGGA TGAACCTAAA AGTGGATTAA ATGAAGACCA AGCCATGATG 
CAGGATTGTT ACAGCAAAAT TGTAGATAAG CTGTCCTCTG CAAACCCCAC CATGGTATTA 
CAGGTTCAGA TGCTCGTGGC TGAACTGCGC AGGGTCACTG TGCTCTGGGA TGAGCTCTGG 
CTGGGAGTTT TGCTGCAACA ACACATGTAT GTCCTGAGAC GAATTCAGCA GCTTGAAGAT 
GAGGTGAAGA GAGTCCAGAA CAACAACACC TTACGCAAAG AAGAGAAAAT TGCAATCATG 
AGGGAGAAGC ACACAGCTTT GATGAAGCCC ATCGTATTTG CTTTGGAGCA TGTGAGGAGT 
ATCACAGCGG CTCCTGCAGA AACACCTCAT GAAAAATGGT TTCAGGATAA CTATGGTGAT 
GCCATTGAAA ATGCCCTAGA AAAACTGAAG ACTCCATTGA ACCCTGCAAA GCCTGGGAGC 
AGCTGGATTC CATTTAAAGA GATAATGCTA AGTTTGCAAC AGAGAGCACA GAAACGTGCA 
AGTTACATCT TGCGTCTTGA AGAAATCAGT CCATGGTTGG CTGCCATGAC TAACACTGAA 
ATTGCTCTTC CTGGGGAAGT CTCAGCCAGA GACACTGTCA CAATCCATAG TGTGGGCGGA 
ACCATCACAA TCTTACCGAC TAAAACCAAG CCAAAGAAAC TTCTCTTTCT TGGATCAGAT 
GGGAAGAGCT ATCCTTATCT TTTCAAAGGA CTGGAGGATT TACATCTGGA TGAGAGAATA 
ATGCAGTTCC TATCTATTGT GAATACCATG TTTGCTACAA TTAATCGCCA AGAAACACCC 
CGGTTCCATG CTCGACACTA TTCTGTAACA CCACTAGGAA CAAGATCAGG ACTAATCCAG 
TGGGTAGATG GAGCCACACC CTTATTTGGT CTTTACAAAC GATGGCAACA ACGGGAAGCT 
GCCTTACAAG CACAAAAGGC CCAAGATTCC TACCAAACTC CTCAGAATCC TGGAATTGTA 
CCCCGTCCTA GTGAACTTTA TTACAGTAAA ATTGGCCCTG CTTTGAAAAC AGTTGGGCTT 
AGCCTGGATG TGTCCCGTCG GGATTGGCCT CTTCATGTAA TGAAGGCAGT ATTGGAAGAG 
TTAATGGAGG CCACACCCCC GAATCTCCTT GCCAAAGAGC TCTGGTCATC TTGCACAACA 
CCTGATGAAT GGTGGAGAGT TACGCAGTCT TATGCAAGAT CTACTGCAGT CATGTCTATG 
GTTGGATACA TAATTGGCCT TGGAGACAGA CATCTGGATA ATGTTCTTAT AGATATGACG 
ACTGGAGAAG TTGTTCACAT AGATTACAAT GTTTGCTTTG AAAAAGGTAA TATAAAAGTA 
GACTTTACAT TTATGAGGGC CAAATGTTTT TTGGTGAAAG GGCAAAAATA TAATGATTTG 
TCTTTTCAAG GTAAAAGCCT TAGAGTTCCT GAGAAAGTAC CTTTTCGAAT GACACAAAAC 
ATTGAAACAG CACTGGGTGT AACTGGAGTA GAAGGTGTAT TTAGGCTTTC ATGTGAGCAG 
GTTTTACACA TTATGCGGCG TGGCAGAGAG ACCCTGCTGA CGCTGCTGGA GGCCTTTGTG 
TACGACCCTC TGGTGGACTG GACAGCAGGA GGCGAGGCTG GGTTTGCTGG TGCTGTCTAT 
GGTGGAGGTG GCCAGCAGGC CGAGAGCAAG CAGAGCAAGA GAGAGATGGA GCGAGAGATC 
ACCCGCAGCC TGTTTTCTTC TAGAGTAGCT GAGATTAAGG TGAACTGGTT TAAGAATAGA 
GATGAGATGC TGGTTGTGCT TCCCAAGTTG GACGGTAGCT TAGATGAATA CCTAAGCTTG 
CAAGAGCAAC TGACAGATGT GGAAAAACTG CAGGGCAAAC TACTGGAGGA AATAGAGTTT 
CTAGAAGGAG CTGAAGGGGT GGATCATCCT TCTCATACTC TGCAACACAG GTATTCTGAG 
CACACCCAAC TACAGACTCA GCAAAGAGCT GTTCAGGAAG CAATCCAGGT GAAGCTGAAT 
GAATTTGAAC AATGGATAAC ACATTATCAG GCTGCATTCA ATAATTTAGA AGCAACACAG 
CTTGCAAGCT TGCTTCAAGA GATAAGCACA CAAATGGACC TTGGTCCTCC AAGTTACGTG 
CCAGCAACAG CCTTTCTGCA GAATGCTGGT CAGGCCCACT TGATTAGCCA GTGCGAGCAG 
CTGGAGGGGG AGGTTGGTGC TCTCCTGCAG CAGAGGCGCT CCGTGCTCCG TGGCTGTCTG 
GAGCAACTGC ATCACTATGC AACCGTGGCC CTGCAGTATC CGAAGGCCAT ATTTCAGAAA 
CATCGAATTG AACAGTGGAA GACCTGGATG GAAGAGCTCA TCTGTAACAC CACAGTAGAG 
CGTTGTCAAG AGCTCTATAG GAAATATGAA ATGCAATATG CTCCCCAGCC ACCCCCAACA 
GTGTGTCAGT TCATCACTGC CACTGAAATG ACCCTGCAGC GATACGCAGC AGACATCAAC 
AGCAGACTTA TTAGACAAGT GGAACGCTTG AAACAGGAAG CTGTCACTGT GCCAGTTTGT 
GAAGATCAGT TGAAAGAAAT TGAACGTTGC ATTAAAGTTT TCCTTCATGA GAATGGAGAA 
GAAGGATCTT TGAGTCTAGC AAGTGTTATT ATTTCTGCCC TTTGTACCCT TACAAGGCGT 
AACCTGATGA TGGAAGGTGC AGCGTCAAGT GCTGGAGAAC AGCTGGTTGA TCTGACTTCT 
CGGGATGGAG CCTGGTTCTT GGAGGAACTC TGCAGTATGA GCGGAAACGT CACCTGCTTG 
GTTCAGTTAC TGAAGCAGTG CCACCTGGTG CCACAGGACT TAGATATCCC GAACCCCATG 
GAAGCGTCTG AGACAGTTCA CTTAGCCAAT GGAGTGTATA CCTCACTTCA GGAATTGAAT 
TCGAATTTCC GGCAAATCAT ATTTCCAGAA GCACTTCGAT GTTTAATGAA AGGGGAATAC 
ACGTTAGAAA GTATGCTGCA TGAACTGGAC GGTCTTATTG AGCAGACCAC CGATGGCGTT 
CCCCTGCAGA CTCTAGTGGA ATCTCTTCAG GCCTACTTAA GAAACGCAGC TATGGGACTG 
GAAGAAGAAA CACATGCTCA TTACATCGAT GTTGCCAGAC TACTACATGC TCAGTACGGT 
GAATTAATCC AACCGAGAAA TGGTTCAGTT GATGAAACAC CCAAAATGTC AGCTGGCCAG 
ATGCTTTTGG TAGCATTCGA TGGCATGTTT GCTCAAGTTG AAACTGCTTT CAGCTTATTA 
GTTGAAAAGT TGAACAAGAT GGAAATTCCC ATAGCTTGGC GAAAGATTGA CATCATAAGG 
GAAGCCAGGA GTACTCAAGT TAATTTTTTT GATGATGATA ATCACCGGCA GGTGCTAGAA 
GAGATTTTCT TTCTAAAAAG ACTACAGACT ATTAAGGAGT TCTTCAGGCT CTGTGGTACC 
TTTTCTAAAA CATTGTCAGG ATCAAGTTCA CTTGAAGATC AGAATACTGT GAATGGGCCT 
GTACAGATTG TCAATGTGAA AACCCTTTTT AGAAACTCTT GTTTCAGTGA AGACCAAATG 
GCCAAACCTA TCAAGGCATT CACAGCTGAC TTTGTGAGGC AGCTCTTGAT AGGGCTACCC 
AACCAAGCCC TCGGACTCAC ACTGTGCAGT TTTATCAGTG CTCTGGGTGT AGACATCATT 
GCTCAAGTAG AGGCAAAGGA CTTTGGTGCC GAAAGCAAAG TTTCTGTTGA TGATCTCTGT 
AAGAAAGCGG TGGAACATAA CATCCAGATA GGGAAGTTCT CTCAGCTGGT TATGAACAGG 
GCAACTGTGT TAGCAAGTTC TTACGACACT GCCTGGAAGA AGCATGACTT GGTGCGAAGG 
CTAGAAACCA GTATTTCTTC TTGTAAGACA AGCCTGCAGC GGGTTCAGCT GCATATTGCC 
ATGTTTCAGT GGCAACATGA AGATCTACTT ATCAATAGAC CACAAGCCAT GTCAGTCACA 
CCTCCCCCAC GGTCTGCTAT CCTAACCAGC ATGAAAAAGA AGCTGCATAC CCTGAGCCAG 
ATTGAAACTT CTATTGCAAC AGTTCAGGAG AAGCTAGCTG CACTTGAATC AAGTATTGAA 
CAGCGACTCA AGTGGGCAGG TGGTGCCAAC CCTGCATTGG CCCCTGTACT ACAAGATTTT 
GAAGCAACGA TAGCTGAAAG AAGAAATCTT GTCCTTAAAG AGAGCCAAAG AGCAAGTCAG 
GTCACATTTC TCTGCAGCAA TATCATTCAT TTTGAAAGTT TACGAACAAG AACTGCAGAA 
GCCTTAAACC TGGATGCGGC GTTATTTGAA CTAATCAAGC GATGTCAGCA GATGTGTTCG 
TTTGCATCAC AGTTTAACAG TTCAGTGTCT GAGTTAGAGC TTCGTTTATT ACAGAGAGTG 
GACACTGGTC TTGAACATCC TATTGGCAGC TCTGAATGGC TTTTGTCAGC ACACAAACAG 
TTGACCCAGG ATATGTCTAC TCAGAGGGCA ATTCAGACAG AGAAAGAGCA GCAGATAGAA 
ACGGTCTGTG AAACAATTCA GAATCTGGTT GATAATATAA AGACTGTGCT CACTGGTCAT 
AACCGACAGC TTGGAGATGT CAAACATCTC TTGAAAGCTA TGGCTAAGGA TGAAGAAGCT 
GCTCTGGCAG ATGGTGAAGA TGTTCCCTAT GAGAACAGTG TTAGGCAGTT TTTGGGTGAA 
TATAAATCAT GGCAAGACAA CATTCAAACA GTTCTATTTA CATTAGTCCA GGCTATGGGT 
CAGGTTCGAA GTCAAGAACA CGTTGAAATG CTCCAGGAAA TCACTCCCAC CTTGAAAGAA 
CTGAAAACAC AAAGTCAGAG TATCTATAAT AATTTAGTGA GTTTTGCATC ACCCTTAGTC 
ACCGATGCAA CAAATGAATG TTCGAGTCCA ACGTCATCTG CTACTTATCA GCCATCCTTC 
GCTGCAGCAG TCCGGAGTAA CACTGGCCAG AAGACTCAGC CTGATGTCAT GTCACAGAAT 
GCTAGAAAGC TGATCCAAAA AAATCTTGCT ACATCAGCTG ATACTCCACC AAGCACCGTT 
CCAGGAACTG GCAAGAGTGT TGCTTGTAGT CCTAAAAAGG CAGTCAGAGA CCCTAAAACT 
GGGAAAGCGG TGCAAGAGAG AAACTCCTAT GCAGTGAGTG TGTGGAAGAG AGTGAAAGCC 
AAGTTAGAGG GCCGAGATGT TGATCCGAAT AGGAGGATGT CAGTTGCTGA ACAGGTTGAC 
TATGTCATTA AGGAAGCAAC TAATCTAGAT AACTTGGCTC AGCTGTATGA AGGTTGGACA 
GCCTGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255240.1
CDS	524..11515
Translation	MRFLRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSVQHGSRALATKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGNIKVDFTFMRAKCFLVKGQKYNDLSFQGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_005255183.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255183.4

ORF Insert Sequence:

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATAATTTAAA ATATTCTTCA 
TCCAGAGATA GAGGTGGTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACGCTGACA TACAGAATGA CGAAAAGGGT 
GGCTACAGTG TCAATGGAGG ATCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAACAATGT GACCAGCCCT GAGTTCACCA GTGTTCAGCA TGGCAGTCGT 
GCTTTAGCCA CCAAAGACAT GAGGAAATCA CAGGAGAGAT CGATGTCTTA TTCTGATGAG 
TCTCGACTGT CGAATCTTCT TCGGAGGATC ACCCGGGAAG ACGACAGAGA CCGAAGATTG 
GCTACTGTAA AGCAGTTGAA AGAATTTATT CAGCAACCAG AAAATAAGCT GGTACTAGTT 
AAACAATTGG ATAATATCTT GGCTGCTGTA CATGACGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAGT TGAGACAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTCTGAGC 
TATGAGGCTG AGAAGATCTT CAAGTGGATT TTTAGCAAAT TTAGCTCATC TGCAAAAGAT 
GAAGTTAAAC TCCTCTACTT ATGTGCCACC TACAAAGCAC TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCTGTAAT GCAGCTTGTA ATGACCAGCC TGCAGTCTAT TCTTGAAAAT 
GTGGATACAC CAGAATTGCT TTGTAAATGT GTTAAGTGCA TTCTTTTGGT GGCTCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACAGTTG ATATATTAGT TGGATGGCAT 
ATAGATCATA CTCAGAAACC TTCGCTCACG CAGCAGGTAT CTGGGTGGTT GCAGAGTTTG 
GAGCCATTTT GGGTAGCTGA TCTTGCATTT TCTACTACTC TTCTTGGTCA GTTTCTGGAA 
GACATGGAAG CATATGCTGA GGACCTCAGC CATGTGGCCT CTGGGGAATC AGTGGATGAA 
GATGTCCCTC CTCCATCAGT GTCATTACCA AAGCTGGCTG CACTTCTCCG GGTATTTAGT 
ACTGTGGTGA GGAGCATTGG GGAACGCTTC AGCCCAATTC GGGGTCCTCC AATTACTGAG 
GCATATGTAA CAGATGTTCT GTACAGAGTA ATGAGATGTG TGACGGCTGC AAACCAGGTG 
TTTTTTTCTG AGGCTGTGTT GACAGCTGCT AATGAGTGTG TTGGTGTTTT GCTCGGCAGC 
TTGGATCCTA GCATGACTAT ACATTGTGAC ATGGTCATTA CATATGGATT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACCGATTAT ATCATCTCAG TCTTGAATTT ACTCACGCTG 
ATTGTTGAAC AGATAAATAC GAAACTGCCA TCATCATTTG TAGAAAAACT GTTTATACCA 
TCATCTAAAC TACTATTCTT GCGTTATCAT AAAGAAAAAG AGGTTGTTGC TGTAGCCCAT 
GCTGTTTATC AAGCAGTGCT CAGCTTGAAG AATATTCCTG TTTTGGAGAC TGCCTATAAG 
TTAATATTGG GAGAAATGAC TTGTGCCCTA AACAACCTCC TACACAGTCT ACAACTTCCT 
GAGGCCTGTT CTGAAATAAA ACATGAGGCT TTTAAGAATC ATGTGTTCAA TGTAGACAAT 
GCAAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACTA CAATTGGAAA TGCCAAAAAC 
TCACTAATAG GGATGTGGGC GCTATCTCCA ACTGTCTTTG CACTTCTGAG TAAGAATCTG 
ATGATTGTGC ACAGTGACCT GGCTGTTCAC TTCCCTGCCA TTCAGTATGC TGTGCTCTAC 
ACATTGTATT CTCATTGTAC CAGGCATGAT CACTTTATCT CTAGTAGCCT CAGTTCTTCC 
TCTCCTTCTT TGTTTGATGG AGCTGTGATT AGCACTGTAA CTACGGCTAC AAAGAAACAT 
TTCTCAATTA TATTAAATCT TCTGGGAATA TTACTTAAGA AAGATAACCT TAACCAGGAC 
ACGAGGAAAC TGTTAATGAC TTGGGCTTTG GAAGCAGCTG TTTTAATGAA GAAGTCTGAA 
ACATACGCAC CTTTATTCTC TCTTCCGTCT TTCCATAAAT TTTGCAAAGG CCTTTTAGCC 
AACACTCTCG TTGAAGATGT GAATATCTGT CTGCAGGCAT GCAGCAGTCT ACATGCTCTG 
TCCTCTTCCT TGCCAGATGA TCTTTTACAG AGATGTGTCG ATGTTTGCCG TGTTCAACTA 
GTGCACAGTG GAACTCGTAT TCGACAAGCA TTTGGAAAAC TGTTGAAATC AATTCCTTTA 
GATGTTGTCC TAAGCAATAA CAATCACACA GAAATTCAAG AAATTTCTTT AGCATTAAGA 
AGTCACATGA GTAAAGCACC AAGTAATACA TTCCACCCCC AAGATTTCTC TGATGTTATT 
AGTTTTATTT TGTATGGGAA CTCTCATAGA ACAGGGAAGG ACAATTGGTT GGAAAGACTG 
TTCTATAGCT GCCAGAGACT GGATAAGCGT GACCAGTCAA CAATTCCACG CAATCTCCTG 
AAGACAGATG CTGTCCTTTG GCAGTGGGCC ATATGGGAAG CTGCACAATT CACTGTTCTT 
TCTAAGCTGA GAACCCCACT GGGCAGAGCT CAAGACACCT TCCAGACAAT TGAAGGTATC 
ATTCGAAGTC TCGCAGCTCA CACATTAAAC CCTGATCAGG ATGTTAGTCA GTGGACAACT 
GCAGACAATG ATGAAGGCCA TGGTAACAAC CAACTTAGAC TTGTTCTTCT TCTGCAGTAT 
CTGGAAAATC TGGAGAAATT AATGTATAAT GCATACGAGG GATGTGCTAA TGCATTAACT 
TCACCTCCCA AGGTCATTAG AACTTTTTTC TATACCAATC GCCAAACTTG TCAGGACTGG 
CTAACGCGGA TTCGACTCTC CATCATGAGG GTAGGATTGT TGGCAGGCCA GCCTGCAGTG 
ACAGTGAGAC ATGGCTTTGA CTTGCTTACA GAGATGAAAA CAACCAGCCT ATCTCAGGGG 
AATGAATTGG AAGTAACCAT TATGATGGTG GTAGAAGCAT TATGTGAACT TCATTGTCCT 
GAAGCTATAC AGGGAATTGC TGTCTGGTCA TCATCTATTG TTGGAAAAAA TCTTCTGTGG 
ATTAACTCAG TGGCTCAACA GGCTGAAGGG AGGTTTGAAA AGGCCTCTGT GGAGTACCAG 
GAACACCTGT GTGCCATGAC AGGTGTTGAT TGCTGCATCT CCAGCTTTGA CAAATCGGTG 
CTCACCTTAG CCAATGCTGG GCGTAACAGT GCCAGCCCGA AACATTCTCT GAATGGTGAA 
TCCAGAAAAA CTGTGCTGTC CAAACCGACT GACTCTTCCC CTGAGGTTAT AAATTATTTA 
GGAAATAAAG CATGTGAGTG CTACATCTCA ATTGCCGATT GGGCTGCTGT GCAGGAATGG 
CAGAACGCTA TCCATGACTT GAAAAAGAGT ACCAGTAGCA CTTCCCTCAA CCTGAAAGCT 
GACTTCAACT ATATAAAATC ATTAAGCAGC TTTGAGTCTG GAAAATTTGT TGAATGTACC 
GAGCAGTTAG AATTGTTACC AGGAGAAAAT ATCAATCTAC TTGCTGGAGG ATCAAAAGAA 
AAAATAGACA TGAAAAAACT GCTTCCTAAC ATGTTAAGTC CGGATCCGAG GGAACTTCAG 
AAATCCATTG AAGTTCAATT GTTAAGAAGT TCTGTTTGTT TGGCAACTGC TTTAAACCCG 
ATAGAACAAG ATCAGAAGTG GCAGTCTATA ACTGAAAATG TGGTAAAGTA CTTGAAGCAA 
ACATCCCGCA TCGCTATTGG ACCTCTGAGA CTTTCTACTT TAACAGTTTC ACAGTCTTTG 
CCAGTTCTAA GTACCTTGCA GCTGTATTGC TCATCTGCTT TGGAGAACAC AGTTTCTAAC 
AGACTTTCAA CAGAGGACTG TCTTATTCCA CTCTTCAGTG AAGCTTTACG TTCATGTAAA 
CAGCATGACG TGAGGCCATG GATGCAGGCA TTAAGGTATA CTATGTACCA GAATCAGTTG 
TTGGAGAAAA TTAAAGAACA AACAGTCCCA ATTAGAAGCC ATCTCATGGA ATTAGGTCTA 
ACAGCAGCAA AATTTGCTAG AAAACGAGGG AATGTGTCCC TTGCAACAAG ACTGCTGGCA 
CAGTGCAGTG AAGTTCAGCT GGGAAAGACC ACCACTGCAC AGGATTTAGT CCAACATTTT 
AAAAAACTAT CAACCCAAGG TCAAGTGGAT GAAAAATGGG GGCCCGAACT TGATATTGAA 
AAAACCAAAT TGCTTTATAC AGCAGGCCAG TCAACACATG CAATGGAAAT GTTGAGTTCT 
TGTGCCATAT CTTTCTGCAA GTCTGTGAAA GCTGAATATG CAGTTGCTAA ATCAATTCTG 
ACACTGGCTA AATGGATCCA GGCAGAATGG AAAGAGATTT CAGGACAGCT GAAACAGGTT 
TACAGAGCTC AGCACCAACA GAACTTCACA GGTCTTTCTA CTTTGTCTAA AAACATACTC 
ACTCTAATAG AACTGCCATC TGTTAATACG ATGGAAGAAG AGTATCCTCG GATCGAGAGT 
GAATCTACAG TGCATATTGG AGTTGGAGAA CCTGACTTCA TTTTGGGACA GTTGTATCAC 
CTGTCTTCAG TACAGGCACC TGAAGTAGCC AAATCTTGGG CAGCGTTGGC CAGCTGGGCT 
TATAGGTGGG GCAGAAAGGT GGTTGACAAT GCCAGTCAGG GAGAAGGTGT TCGTCTGCTG 
CCTAGAGAAA AATCTGAAGT TCAGAATCTA CTTCCAGACA CTATAACTGA GGAAGAGAAA 
GAGAGAATAT ATGGTATTCT TGGACAGGCT GTGTGTCGGC CGGCGGGGAT TCAGGATGAA 
GATATAACAC TTCAGATAAC TGAGAGTGAA GACAACGAAG AAGATGACAT GGTTGATGTT 
ATCTGGCGTC AGTTGATATC AAGCTGCCCA TGGCTTTCAG AACTTGATGA AAGTGCAACT 
GAAGGAGTTA TTAAAGTGTG GAGGAAAGTT GTAGATAGAA TATTCAGCCT GTACAAACTC 
TCTTGCAGTG CATACTTTAC TTTCCTTAAA CTCAACGCTG GTCAAATTCC TTTAGATGAG 
GATGACCCTA GGCTGCATTT AAGTCACAGA GTGGAACAGA GCACTGATGA CATGATTGTG 
ATGGCCACAT TGCGCCTGCT GCGGTTGCTC GTGAAGCATG CTGGTGAGCT TCGGCAGTAT 
CTGGAGCACG GCTTGGAGAC AACACCCACT GCACCATGGA GAGGAATTAT TCCGCAACTT 
TTCTCACGCT TAAACCACCC TGAAGTGTAT GTGCGCCAAA GTATTTGTAA CCTTCTCTGC 
CGTGTGGCTC AAGATTCCCC ACATCTCATA TTGTATCCTG CAATAGTGGG TACCATATCG 
CTTAGTAGTG AATCCCAGGC TTCAGGAAAT AAATTTTCCA CTGCAATTCC AACTTTACTT 
GGCAATATTC AAGGAGAAGA ATTGCTGGTT TCTGAATGTG AGGGAGGAAG TCCTCCTGCA 
TCTCAGGATA GCAATAAGGA TGAACCTAAA AGTGGATTAA ATGAAGACCA AGCCATGATG 
CAGGATTGTT ACAGCAAAAT TGTAGATAAG CTGTCCTCTG CAAACCCCAC CATGGTATTA 
CAGGTTCAGA TGCTCGTGGC TGAACTGCGC AGGGTCACTG TGCTCTGGGA TGAGCTCTGG 
CTGGGAGTTT TGCTGCAACA ACACATGTAT GTCCTGAGAC GAATTCAGCA GCTTGAAGAT 
GAGGTGAAGA GAGTCCAGAA CAACAACACC TTACGCAAAG AAGAGAAAAT TGCAATCATG 
AGGGAGAAGC ACACAGCTTT GATGAAGCCC ATCGTATTTG CTTTGGAGCA TGTGAGGAGT 
ATCACAGCGG CTCCTGCAGA AACACCTCAT GAAAAATGGT TTCAGGATAA CTATGGTGAT 
GCCATTGAAA ATGCCCTAGA AAAACTGAAG ACTCCATTGA ACCCTGCAAA GCCTGGGAGC 
AGCTGGATTC CATTTAAAGA GATAATGCTA AGTTTGCAAC AGAGAGCACA GAAACGTGCA 
AGTTACATCT TGCGTCTTGA AGAAATCAGT CCATGGTTGG CTGCCATGAC TAACACTGAA 
ATTGCTCTTC CTGGGGAAGT CTCAGCCAGA GACACTGTCA CAATCCATAG TGTGGGCGGA 
ACCATCACAA TCTTACCGAC TAAAACCAAG CCAAAGAAAC TTCTCTTTCT TGGATCAGAT 
GGGAAGAGCT ATCCTTATCT TTTCAAAGGA CTGGAGGATT TACATCTGGA TGAGAGAATA 
ATGCAGTTCC TATCTATTGT GAATACCATG TTTGCTACAA TTAATCGCCA AGAAACACCC 
CGGTTCCATG CTCGACACTA TTCTGTAACA CCACTAGGAA CAAGATCAGG ACTAATCCAG 
TGGGTAGATG GAGCCACACC CTTATTTGGT CTTTACAAAC GATGGCAACA ACGGGAAGCT 
GCCTTACAAG CACAAAAGGC CCAAGATTCC TACCAAACTC CTCAGAATCC TGGAATTGTA 
CCCCGTCCTA GTGAACTTTA TTACAGTAAA ATTGGCCCTG CTTTGAAAAC AGTTGGGCTT 
AGCCTGGATG TGTCCCGTCG GGATTGGCCT CTTCATGTAA TGAAGGCAGT ATTGGAAGAG 
TTAATGGAGG CCACACCCCC GAATCTCCTT GCCAAAGAGC TCTGGTCATC TTGCACAACA 
CCTGATGAAT GGTGGAGAGT TACGCAGTCT TATGCAAGAT CTACTGCAGT CATGTCTATG 
GTTGGATACA TAATTGGCCT TGGAGACAGA CATCTGGATA ATGTTCTTAT AGATATGACG 
ACTGGAGAAG TTGTTCACAT AGATTACAAT GTTTGCTTTG AAAAAGGTAA TATAAAAGTA 
GACTTTACAT TTATGAGGGC CAAATGTTTT TTGGTGAAAG GGCAAAAATA TAATGATTTG 
TCTTTTCAAG GTAAAAGCCT TAGAGTTCCT GAGAAAGTAC CTTTTCGAAT GACACAAAAC 
ATTGAAACAG CACTGGGTGT AACTGGAGTA GAAGGTGTAT TTAGGCTTTC ATGTGAGCAG 
GTTTTACACA TTATGCGGCG TGGCAGAGAG ACCCTGCTGA CGCTGCTGGA GGCCTTTGTG 
TACGACCCTC TGGTGGACTG GACAGCAGGA GGCGAGGCTG GGTTTGCTGG TGCTGTCTAT 
GGTGGAGGTG GCCAGCAGGC CGAGAGCAAG CAGAGCAAGA GAGAGATGGA GCGAGAGATC 
ACCCGCAGCC TGTTTTCTTC TAGAGTAGCT GAGATTAAGG TGAACTGGTT TAAGAATAGA 
GATGAGATGC TGGTTGTGCT TCCCAAGTTG GACGGTAGCT TAGATGAATA CCTAAGCTTG 
CAAGAGCAAC TGACAGATGT GGAAAAACTG CAGGGCAAAC TACTGGAGGA AATAGAGTTT 
CTAGAAGGAG CTGAAGGGGT GGATCATCCT TCTCATACTC TGCAACACAG GTATTCTGAG 
CACACCCAAC TACAGACTCA GCAAAGAGCT GTTCAGGAAG CAATCCAGGT GAAGCTGAAT 
GAATTTGAAC AATGGATAAC ACATTATCAG GCTGCATTCA ATAATTTAGA AGCAACACAG 
CTTGCAAGCT TGCTTCAAGA GATAAGCACA CAAATGGACC TTGGTCCTCC AAGTTACGTG 
CCAGCAACAG CCTTTCTGCA GAATGCTGGT CAGGCCCACT TGATTAGCCA GTGCGAGCAG 
CTGGAGGGGG AGGTTGGTGC TCTCCTGCAG CAGAGGCGCT CCGTGCTCCG TGGCTGTCTG 
GAGCAACTGC ATCACTATGC AACCGTGGCC CTGCAGTATC CGAAGGCCAT ATTTCAGAAA 
CATCGAATTG AACAGTGGAA GACCTGGATG GAAGAGCTCA TCTGTAACAC CACAGTAGAG 
CGTTGTCAAG AGCTCTATAG GAAATATGAA ATGCAATATG CTCCCCAGCC ACCCCCAACA 
GTGTGTCAGT TCATCACTGC CACTGAAATG ACCCTGCAGC GATACGCAGC AGACATCAAC 
AGCAGACTTA TTAGACAAGT GGAACGCTTG AAACAGGAAG CTGTCACTGT GCCAGTTTGT 
GAAGATCAGT TGAAAGAAAT TGAACGTTGC ATTAAAGTTT TCCTTCATGA GAATGGAGAA 
GAAGGATCTT TGAGTCTAGC AAGTGTTATT ATTTCTGCCC TTTGTACCCT TACAAGGCGT 
AACCTGATGA TGGAAGGTGC AGCGTCAAGT GCTGGAGAAC AGCTGGTTGA TCTGACTTCT 
CGGGATGGAG CCTGGTTCTT GGAGGAACTC TGCAGTATGA GCGGAAACGT CACCTGCTTG 
GTTCAGTTAC TGAAGCAGTG CCACCTGGTG CCACAGGACT TAGATATCCC GAACCCCATG 
GAAGCGTCTG AGACAGTTCA CTTAGCCAAT GGAGTGTATA CCTCACTTCA GGAATTGAAT 
TCGAATTTCC GGCAAATCAT ATTTCCAGAA GCACTTCGAT GTTTAATGAA AGGGGAATAC 
ACGTTAGAAA GTATGCTGCA TGAACTGGAC GGTCTTATTG AGCAGACCAC CGATGGCGTT 
CCCCTGCAGA CTCTAGTGGA ATCTCTTCAG GCCTACTTAA GAAACGCAGC TATGGGACTG 
GAAGAAGAAA CACATGCTCA TTACATCGAT GTTGCCAGAC TACTACATGC TCAGTACGGT 
GAATTAATCC AACCGAGAAA TGGTTCAGTT GATGAAACAC CCAAAATGTC AGCTGGCCAG 
ATGCTTTTGG TAGCATTCGA TGGCATGTTT GCTCAAGTTG AAACTGCTTT CAGCTTATTA 
GTTGAAAAGT TGAACAAGAT GGAAATTCCC ATAGCTTGGC GAAAGATTGA CATCATAAGG 
GAAGCCAGGA GTACTCAAGT TAATTTTTTT GATGATGATA ATCACCGGCA GGTGCTAGAA 
GAGATTTTCT TTCTAAAAAG ACTACAGACT ATTAAGGAGT TCTTCAGGCT CTGTGGTACC 
TTTTCTAAAA CATTGTCAGG ATCAAGTTCA CTTGAAGATC AGAATACTGT GAATGGGCCT 
GTACAGATTG TCAATGTGAA AACCCTTTTT AGAAACTCTT GTTTCAGTGA AGACCAAATG 
GCCAAACCTA TCAAGGCATT CACAGCTGAC TTTGTGAGGC AGCTCTTGAT AGGGCTACCC 
AACCAAGCCC TCGGACTCAC ACTGTGCAGT TTTATCAGTG CTCTGGGTGT AGACATCATT 
GCTCAAGTAG AGGCAAAGGA CTTTGGTGCC GAAAGCAAAG TTTCTGTTGA TGATCTCTGT 
AAGAAAGCGG TGGAACATAA CATCCAGATA GGGAAGTTCT CTCAGCTGGT TATGAACAGG 
GCAACTGTGT TAGCAAGTTC TTACGACACT GCCTGGAAGA AGCATGACTT GGTGCGAAGG 
CTAGAAACCA GTATTTCTTC TTGTAAGACA AGCCTGCAGC GGGTTCAGCT GCATATTGCC 
ATGTTTCAGT GGCAACATGA AGATCTACTT ATCAATAGAC CACAAGCCAT GTCAGTCACA 
CCTCCCCCAC GGTCTGCTAT CCTAACCAGC ATGAAAAAGA AGCTGCATAC CCTGAGCCAG 
ATTGAAACTT CTATTGCAAC AGTTCAGGAG AAGCTAGCTG CACTTGAATC AAGTATTGAA 
CAGCGACTCA AGTGGGCAGG TGGTGCCAAC CCTGCATTGG CCCCTGTACT ACAAGATTTT 
GAAGCAACGA TAGCTGAAAG AAGAAATCTT GTCCTTAAAG AGAGCCAAAG AGCAAGTCAG 
GTCACATTTC TCTGCAGCAA TATCATTCAT TTTGAAAGTT TACGAACAAG AACTGCAGAA 
GCCTTAAACC TGGATGCGGC GTTATTTGAA CTAATCAAGC GATGTCAGCA GATGTGTTCG 
TTTGCATCAC AGTTTAACAG TTCAGTGTCT GAGTTAGAGC TTCGTTTATT ACAGAGAGTG 
GACACTGGTC TTGAACATCC TATTGGCAGC TCTGAATGGC TTTTGTCAGC ACACAAACAG 
TTGACCCAGG ATATGTCTAC TCAGAGGGCA ATTCAGACAG AGAAAGAGCA GCAGATAGAA 
ACGGTCTGTG AAACAATTCA GAATCTGGTT GATAATATAA AGACTGTGCT CACTGGTCAT 
AACCGACAGC TTGGAGATGT CAAACATCTC TTGAAAGCTA TGGCTAAGGA TGAAGAAGCT 
GCTCTGGCAG ATGGTGAAGA TGTTCCCTAT GAGAACAGTG TTAGGCAGTT TTTGGGTGAA 
TATAAATCAT GGCAAGACAA CATTCAAACA GTTCTATTTA CATTAGTCCA GGCTATGGGT 
CAGGTTCGAA GTCAAGAACA CGTTGAAATG CTCCAGGAAA TCACTCCCAC CTTGAAAGAA 
CTGAAAACAC AAAGTCAGAG TATCTATAAT AATTTAGTGA GTTTTGCATC ACCCTTAGTC 
ACCGATGCAA CAAATGAATG TTCGAGTCCA ACGTCATCTG CTACTTATCA GCCATCCTTC 
GCTGCAGCAG TCCGGAGTAA CACTGGCCAG AAGACTCAGC CTGATGTCAT GTCACAGAAT 
GCTAGAAAGC TGATCCAAAA AAATCTTGCT ACATCAGCTG ATACTCCACC AAGCACCGTT 
CCAGGAACTG GCAAGAGTGT TGCTTGTAGT CCTAAAAAGG CAGTCAGAGA CCCTAAAACT 
GGGAAAGCGG TGCAAGAGAG AAACTCCTAT GCAGTGAGTG TGTGGAAGAG AGTGAAAGCC 
AAGTTAGAGG GCCGAGATGT TGATCCGAAT AGGAGGATGT CAGTTGCTGA ACAGGTTGAC 
TATGTCATTA AGGAAGCAAC TAATCTAGAT AACTTGGCTC AGCTGTATGA AGGTTGGACA 
GCCTGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_005255183.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255240.1 Homo sapiens SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X3, mRNA.

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CloneID	OHu49089
Clone ID Related Accession (Same CDS sequence)	XM_017023066.1 , XM_005255183.4 , XM_005255183.3 , XM_017023066.2
Accession Version	XM_005255183.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005255183.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATAATTTAAA ATATTCTTCA 
TCCAGAGATA GAGGTGGTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACGCTGACA TACAGAATGA CGAAAAGGGT 
GGCTACAGTG TCAATGGAGG ATCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAACAATGT GACCAGCCCT GAGTTCACCA GTGTTCAGCA TGGCAGTCGT 
GCTTTAGCCA CCAAAGACAT GAGGAAATCA CAGGAGAGAT CGATGTCTTA TTCTGATGAG 
TCTCGACTGT CGAATCTTCT TCGGAGGATC ACCCGGGAAG ACGACAGAGA CCGAAGATTG 
GCTACTGTAA AGCAGTTGAA AGAATTTATT CAGCAACCAG AAAATAAGCT GGTACTAGTT 
AAACAATTGG ATAATATCTT GGCTGCTGTA CATGACGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAGT TGAGACAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTCTGAGC 
TATGAGGCTG AGAAGATCTT CAAGTGGATT TTTAGCAAAT TTAGCTCATC TGCAAAAGAT 
GAAGTTAAAC TCCTCTACTT ATGTGCCACC TACAAAGCAC TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCTGTAAT GCAGCTTGTA ATGACCAGCC TGCAGTCTAT TCTTGAAAAT 
GTGGATACAC CAGAATTGCT TTGTAAATGT GTTAAGTGCA TTCTTTTGGT GGCTCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACAGTTG ATATATTAGT TGGATGGCAT 
ATAGATCATA CTCAGAAACC TTCGCTCACG CAGCAGGTAT CTGGGTGGTT GCAGAGTTTG 
GAGCCATTTT GGGTAGCTGA TCTTGCATTT TCTACTACTC TTCTTGGTCA GTTTCTGGAA 
GACATGGAAG CATATGCTGA GGACCTCAGC CATGTGGCCT CTGGGGAATC AGTGGATGAA 
GATGTCCCTC CTCCATCAGT GTCATTACCA AAGCTGGCTG CACTTCTCCG GGTATTTAGT 
ACTGTGGTGA GGAGCATTGG GGAACGCTTC AGCCCAATTC GGGGTCCTCC AATTACTGAG 
GCATATGTAA CAGATGTTCT GTACAGAGTA ATGAGATGTG TGACGGCTGC AAACCAGGTG 
TTTTTTTCTG AGGCTGTGTT GACAGCTGCT AATGAGTGTG TTGGTGTTTT GCTCGGCAGC 
TTGGATCCTA GCATGACTAT ACATTGTGAC ATGGTCATTA CATATGGATT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACCGATTAT ATCATCTCAG TCTTGAATTT ACTCACGCTG 
ATTGTTGAAC AGATAAATAC GAAACTGCCA TCATCATTTG TAGAAAAACT GTTTATACCA 
TCATCTAAAC TACTATTCTT GCGTTATCAT AAAGAAAAAG AGGTTGTTGC TGTAGCCCAT 
GCTGTTTATC AAGCAGTGCT CAGCTTGAAG AATATTCCTG TTTTGGAGAC TGCCTATAAG 
TTAATATTGG GAGAAATGAC TTGTGCCCTA AACAACCTCC TACACAGTCT ACAACTTCCT 
GAGGCCTGTT CTGAAATAAA ACATGAGGCT TTTAAGAATC ATGTGTTCAA TGTAGACAAT 
GCAAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACTA CAATTGGAAA TGCCAAAAAC 
TCACTAATAG GGATGTGGGC GCTATCTCCA ACTGTCTTTG CACTTCTGAG TAAGAATCTG 
ATGATTGTGC ACAGTGACCT GGCTGTTCAC TTCCCTGCCA TTCAGTATGC TGTGCTCTAC 
ACATTGTATT CTCATTGTAC CAGGCATGAT CACTTTATCT CTAGTAGCCT CAGTTCTTCC 
TCTCCTTCTT TGTTTGATGG AGCTGTGATT AGCACTGTAA CTACGGCTAC AAAGAAACAT 
TTCTCAATTA TATTAAATCT TCTGGGAATA TTACTTAAGA AAGATAACCT TAACCAGGAC 
ACGAGGAAAC TGTTAATGAC TTGGGCTTTG GAAGCAGCTG TTTTAATGAA GAAGTCTGAA 
ACATACGCAC CTTTATTCTC TCTTCCGTCT TTCCATAAAT TTTGCAAAGG CCTTTTAGCC 
AACACTCTCG TTGAAGATGT GAATATCTGT CTGCAGGCAT GCAGCAGTCT ACATGCTCTG 
TCCTCTTCCT TGCCAGATGA TCTTTTACAG AGATGTGTCG ATGTTTGCCG TGTTCAACTA 
GTGCACAGTG GAACTCGTAT TCGACAAGCA TTTGGAAAAC TGTTGAAATC AATTCCTTTA 
GATGTTGTCC TAAGCAATAA CAATCACACA GAAATTCAAG AAATTTCTTT AGCATTAAGA 
AGTCACATGA GTAAAGCACC AAGTAATACA TTCCACCCCC AAGATTTCTC TGATGTTATT 
AGTTTTATTT TGTATGGGAA CTCTCATAGA ACAGGGAAGG ACAATTGGTT GGAAAGACTG 
TTCTATAGCT GCCAGAGACT GGATAAGCGT GACCAGTCAA CAATTCCACG CAATCTCCTG 
AAGACAGATG CTGTCCTTTG GCAGTGGGCC ATATGGGAAG CTGCACAATT CACTGTTCTT 
TCTAAGCTGA GAACCCCACT GGGCAGAGCT CAAGACACCT TCCAGACAAT TGAAGGTATC 
ATTCGAAGTC TCGCAGCTCA CACATTAAAC CCTGATCAGG ATGTTAGTCA GTGGACAACT 
GCAGACAATG ATGAAGGCCA TGGTAACAAC CAACTTAGAC TTGTTCTTCT TCTGCAGTAT 
CTGGAAAATC TGGAGAAATT AATGTATAAT GCATACGAGG GATGTGCTAA TGCATTAACT 
TCACCTCCCA AGGTCATTAG AACTTTTTTC TATACCAATC GCCAAACTTG TCAGGACTGG 
CTAACGCGGA TTCGACTCTC CATCATGAGG GTAGGATTGT TGGCAGGCCA GCCTGCAGTG 
ACAGTGAGAC ATGGCTTTGA CTTGCTTACA GAGATGAAAA CAACCAGCCT ATCTCAGGGG 
AATGAATTGG AAGTAACCAT TATGATGGTG GTAGAAGCAT TATGTGAACT TCATTGTCCT 
GAAGCTATAC AGGGAATTGC TGTCTGGTCA TCATCTATTG TTGGAAAAAA TCTTCTGTGG 
ATTAACTCAG TGGCTCAACA GGCTGAAGGG AGGTTTGAAA AGGCCTCTGT GGAGTACCAG 
GAACACCTGT GTGCCATGAC AGGTGTTGAT TGCTGCATCT CCAGCTTTGA CAAATCGGTG 
CTCACCTTAG CCAATGCTGG GCGTAACAGT GCCAGCCCGA AACATTCTCT GAATGGTGAA 
TCCAGAAAAA CTGTGCTGTC CAAACCGACT GACTCTTCCC CTGAGGTTAT AAATTATTTA 
GGAAATAAAG CATGTGAGTG CTACATCTCA ATTGCCGATT GGGCTGCTGT GCAGGAATGG 
CAGAACGCTA TCCATGACTT GAAAAAGAGT ACCAGTAGCA CTTCCCTCAA CCTGAAAGCT 
GACTTCAACT ATATAAAATC ATTAAGCAGC TTTGAGTCTG GAAAATTTGT TGAATGTACC 
GAGCAGTTAG AATTGTTACC AGGAGAAAAT ATCAATCTAC TTGCTGGAGG ATCAAAAGAA 
AAAATAGACA TGAAAAAACT GCTTCCTAAC ATGTTAAGTC CGGATCCGAG GGAACTTCAG 
AAATCCATTG AAGTTCAATT GTTAAGAAGT TCTGTTTGTT TGGCAACTGC TTTAAACCCG 
ATAGAACAAG ATCAGAAGTG GCAGTCTATA ACTGAAAATG TGGTAAAGTA CTTGAAGCAA 
ACATCCCGCA TCGCTATTGG ACCTCTGAGA CTTTCTACTT TAACAGTTTC ACAGTCTTTG 
CCAGTTCTAA GTACCTTGCA GCTGTATTGC TCATCTGCTT TGGAGAACAC AGTTTCTAAC 
AGACTTTCAA CAGAGGACTG TCTTATTCCA CTCTTCAGTG AAGCTTTACG TTCATGTAAA 
CAGCATGACG TGAGGCCATG GATGCAGGCA TTAAGGTATA CTATGTACCA GAATCAGTTG 
TTGGAGAAAA TTAAAGAACA AACAGTCCCA ATTAGAAGCC ATCTCATGGA ATTAGGTCTA 
ACAGCAGCAA AATTTGCTAG AAAACGAGGG AATGTGTCCC TTGCAACAAG ACTGCTGGCA 
CAGTGCAGTG AAGTTCAGCT GGGAAAGACC ACCACTGCAC AGGATTTAGT CCAACATTTT 
AAAAAACTAT CAACCCAAGG TCAAGTGGAT GAAAAATGGG GGCCCGAACT TGATATTGAA 
AAAACCAAAT TGCTTTATAC AGCAGGCCAG TCAACACATG CAATGGAAAT GTTGAGTTCT 
TGTGCCATAT CTTTCTGCAA GTCTGTGAAA GCTGAATATG CAGTTGCTAA ATCAATTCTG 
ACACTGGCTA AATGGATCCA GGCAGAATGG AAAGAGATTT CAGGACAGCT GAAACAGGTT 
TACAGAGCTC AGCACCAACA GAACTTCACA GGTCTTTCTA CTTTGTCTAA AAACATACTC 
ACTCTAATAG AACTGCCATC TGTTAATACG ATGGAAGAAG AGTATCCTCG GATCGAGAGT 
GAATCTACAG TGCATATTGG AGTTGGAGAA CCTGACTTCA TTTTGGGACA GTTGTATCAC 
CTGTCTTCAG TACAGGCACC TGAAGTAGCC AAATCTTGGG CAGCGTTGGC CAGCTGGGCT 
TATAGGTGGG GCAGAAAGGT GGTTGACAAT GCCAGTCAGG GAGAAGGTGT TCGTCTGCTG 
CCTAGAGAAA AATCTGAAGT TCAGAATCTA CTTCCAGACA CTATAACTGA GGAAGAGAAA 
GAGAGAATAT ATGGTATTCT TGGACAGGCT GTGTGTCGGC CGGCGGGGAT TCAGGATGAA 
GATATAACAC TTCAGATAAC TGAGAGTGAA GACAACGAAG AAGATGACAT GGTTGATGTT 
ATCTGGCGTC AGTTGATATC AAGCTGCCCA TGGCTTTCAG AACTTGATGA AAGTGCAACT 
GAAGGAGTTA TTAAAGTGTG GAGGAAAGTT GTAGATAGAA TATTCAGCCT GTACAAACTC 
TCTTGCAGTG CATACTTTAC TTTCCTTAAA CTCAACGCTG GTCAAATTCC TTTAGATGAG 
GATGACCCTA GGCTGCATTT AAGTCACAGA GTGGAACAGA GCACTGATGA CATGATTGTG 
ATGGCCACAT TGCGCCTGCT GCGGTTGCTC GTGAAGCATG CTGGTGAGCT TCGGCAGTAT 
CTGGAGCACG GCTTGGAGAC AACACCCACT GCACCATGGA GAGGAATTAT TCCGCAACTT 
TTCTCACGCT TAAACCACCC TGAAGTGTAT GTGCGCCAAA GTATTTGTAA CCTTCTCTGC 
CGTGTGGCTC AAGATTCCCC ACATCTCATA TTGTATCCTG CAATAGTGGG TACCATATCG 
CTTAGTAGTG AATCCCAGGC TTCAGGAAAT AAATTTTCCA CTGCAATTCC AACTTTACTT 
GGCAATATTC AAGGAGAAGA ATTGCTGGTT TCTGAATGTG AGGGAGGAAG TCCTCCTGCA 
TCTCAGGATA GCAATAAGGA TGAACCTAAA AGTGGATTAA ATGAAGACCA AGCCATGATG 
CAGGATTGTT ACAGCAAAAT TGTAGATAAG CTGTCCTCTG CAAACCCCAC CATGGTATTA 
CAGGTTCAGA TGCTCGTGGC TGAACTGCGC AGGGTCACTG TGCTCTGGGA TGAGCTCTGG 
CTGGGAGTTT TGCTGCAACA ACACATGTAT GTCCTGAGAC GAATTCAGCA GCTTGAAGAT 
GAGGTGAAGA GAGTCCAGAA CAACAACACC TTACGCAAAG AAGAGAAAAT TGCAATCATG 
AGGGAGAAGC ACACAGCTTT GATGAAGCCC ATCGTATTTG CTTTGGAGCA TGTGAGGAGT 
ATCACAGCGG CTCCTGCAGA AACACCTCAT GAAAAATGGT TTCAGGATAA CTATGGTGAT 
GCCATTGAAA ATGCCCTAGA AAAACTGAAG ACTCCATTGA ACCCTGCAAA GCCTGGGAGC 
AGCTGGATTC CATTTAAAGA GATAATGCTA AGTTTGCAAC AGAGAGCACA GAAACGTGCA 
AGTTACATCT TGCGTCTTGA AGAAATCAGT CCATGGTTGG CTGCCATGAC TAACACTGAA 
ATTGCTCTTC CTGGGGAAGT CTCAGCCAGA GACACTGTCA CAATCCATAG TGTGGGCGGA 
ACCATCACAA TCTTACCGAC TAAAACCAAG CCAAAGAAAC TTCTCTTTCT TGGATCAGAT 
GGGAAGAGCT ATCCTTATCT TTTCAAAGGA CTGGAGGATT TACATCTGGA TGAGAGAATA 
ATGCAGTTCC TATCTATTGT GAATACCATG TTTGCTACAA TTAATCGCCA AGAAACACCC 
CGGTTCCATG CTCGACACTA TTCTGTAACA CCACTAGGAA CAAGATCAGG ACTAATCCAG 
TGGGTAGATG GAGCCACACC CTTATTTGGT CTTTACAAAC GATGGCAACA ACGGGAAGCT 
GCCTTACAAG CACAAAAGGC CCAAGATTCC TACCAAACTC CTCAGAATCC TGGAATTGTA 
CCCCGTCCTA GTGAACTTTA TTACAGTAAA ATTGGCCCTG CTTTGAAAAC AGTTGGGCTT 
AGCCTGGATG TGTCCCGTCG GGATTGGCCT CTTCATGTAA TGAAGGCAGT ATTGGAAGAG 
TTAATGGAGG CCACACCCCC GAATCTCCTT GCCAAAGAGC TCTGGTCATC TTGCACAACA 
CCTGATGAAT GGTGGAGAGT TACGCAGTCT TATGCAAGAT CTACTGCAGT CATGTCTATG 
GTTGGATACA TAATTGGCCT TGGAGACAGA CATCTGGATA ATGTTCTTAT AGATATGACG 
ACTGGAGAAG TTGTTCACAT AGATTACAAT GTTTGCTTTG AAAAAGGTAA TATAAAAGTA 
GACTTTACAT TTATGAGGGC CAAATGTTTT TTGGTGAAAG GGCAAAAATA TAATGATTTG 
TCTTTTCAAG GTAAAAGCCT TAGAGTTCCT GAGAAAGTAC CTTTTCGAAT GACACAAAAC 
ATTGAAACAG CACTGGGTGT AACTGGAGTA GAAGGTGTAT TTAGGCTTTC ATGTGAGCAG 
GTTTTACACA TTATGCGGCG TGGCAGAGAG ACCCTGCTGA CGCTGCTGGA GGCCTTTGTG 
TACGACCCTC TGGTGGACTG GACAGCAGGA GGCGAGGCTG GGTTTGCTGG TGCTGTCTAT 
GGTGGAGGTG GCCAGCAGGC CGAGAGCAAG CAGAGCAAGA GAGAGATGGA GCGAGAGATC 
ACCCGCAGCC TGTTTTCTTC TAGAGTAGCT GAGATTAAGG TGAACTGGTT TAAGAATAGA 
GATGAGATGC TGGTTGTGCT TCCCAAGTTG GACGGTAGCT TAGATGAATA CCTAAGCTTG 
CAAGAGCAAC TGACAGATGT GGAAAAACTG CAGGGCAAAC TACTGGAGGA AATAGAGTTT 
CTAGAAGGAG CTGAAGGGGT GGATCATCCT TCTCATACTC TGCAACACAG GTATTCTGAG 
CACACCCAAC TACAGACTCA GCAAAGAGCT GTTCAGGAAG CAATCCAGGT GAAGCTGAAT 
GAATTTGAAC AATGGATAAC ACATTATCAG GCTGCATTCA ATAATTTAGA AGCAACACAG 
CTTGCAAGCT TGCTTCAAGA GATAAGCACA CAAATGGACC TTGGTCCTCC AAGTTACGTG 
CCAGCAACAG CCTTTCTGCA GAATGCTGGT CAGGCCCACT TGATTAGCCA GTGCGAGCAG 
CTGGAGGGGG AGGTTGGTGC TCTCCTGCAG CAGAGGCGCT CCGTGCTCCG TGGCTGTCTG 
GAGCAACTGC ATCACTATGC AACCGTGGCC CTGCAGTATC CGAAGGCCAT ATTTCAGAAA 
CATCGAATTG AACAGTGGAA GACCTGGATG GAAGAGCTCA TCTGTAACAC CACAGTAGAG 
CGTTGTCAAG AGCTCTATAG GAAATATGAA ATGCAATATG CTCCCCAGCC ACCCCCAACA 
GTGTGTCAGT TCATCACTGC CACTGAAATG ACCCTGCAGC GATACGCAGC AGACATCAAC 
AGCAGACTTA TTAGACAAGT GGAACGCTTG AAACAGGAAG CTGTCACTGT GCCAGTTTGT 
GAAGATCAGT TGAAAGAAAT TGAACGTTGC ATTAAAGTTT TCCTTCATGA GAATGGAGAA 
GAAGGATCTT TGAGTCTAGC AAGTGTTATT ATTTCTGCCC TTTGTACCCT TACAAGGCGT 
AACCTGATGA TGGAAGGTGC AGCGTCAAGT GCTGGAGAAC AGCTGGTTGA TCTGACTTCT 
CGGGATGGAG CCTGGTTCTT GGAGGAACTC TGCAGTATGA GCGGAAACGT CACCTGCTTG 
GTTCAGTTAC TGAAGCAGTG CCACCTGGTG CCACAGGACT TAGATATCCC GAACCCCATG 
GAAGCGTCTG AGACAGTTCA CTTAGCCAAT GGAGTGTATA CCTCACTTCA GGAATTGAAT 
TCGAATTTCC GGCAAATCAT ATTTCCAGAA GCACTTCGAT GTTTAATGAA AGGGGAATAC 
ACGTTAGAAA GTATGCTGCA TGAACTGGAC GGTCTTATTG AGCAGACCAC CGATGGCGTT 
CCCCTGCAGA CTCTAGTGGA ATCTCTTCAG GCCTACTTAA GAAACGCAGC TATGGGACTG 
GAAGAAGAAA CACATGCTCA TTACATCGAT GTTGCCAGAC TACTACATGC TCAGTACGGT 
GAATTAATCC AACCGAGAAA TGGTTCAGTT GATGAAACAC CCAAAATGTC AGCTGGCCAG 
ATGCTTTTGG TAGCATTCGA TGGCATGTTT GCTCAAGTTG AAACTGCTTT CAGCTTATTA 
GTTGAAAAGT TGAACAAGAT GGAAATTCCC ATAGCTTGGC GAAAGATTGA CATCATAAGG 
GAAGCCAGGA GTACTCAAGT TAATTTTTTT GATGATGATA ATCACCGGCA GGTGCTAGAA 
GAGATTTTCT TTCTAAAAAG ACTACAGACT ATTAAGGAGT TCTTCAGGCT CTGTGGTACC 
TTTTCTAAAA CATTGTCAGG ATCAAGTTCA CTTGAAGATC AGAATACTGT GAATGGGCCT 
GTACAGATTG TCAATGTGAA AACCCTTTTT AGAAACTCTT GTTTCAGTGA AGACCAAATG 
GCCAAACCTA TCAAGGCATT CACAGCTGAC TTTGTGAGGC AGCTCTTGAT AGGGCTACCC 
AACCAAGCCC TCGGACTCAC ACTGTGCAGT TTTATCAGTG CTCTGGGTGT AGACATCATT 
GCTCAAGTAG AGGCAAAGGA CTTTGGTGCC GAAAGCAAAG TTTCTGTTGA TGATCTCTGT 
AAGAAAGCGG TGGAACATAA CATCCAGATA GGGAAGTTCT CTCAGCTGGT TATGAACAGG 
GCAACTGTGT TAGCAAGTTC TTACGACACT GCCTGGAAGA AGCATGACTT GGTGCGAAGG 
CTAGAAACCA GTATTTCTTC TTGTAAGACA AGCCTGCAGC GGGTTCAGCT GCATATTGCC 
ATGTTTCAGT GGCAACATGA AGATCTACTT ATCAATAGAC CACAAGCCAT GTCAGTCACA 
CCTCCCCCAC GGTCTGCTAT CCTAACCAGC ATGAAAAAGA AGCTGCATAC CCTGAGCCAG 
ATTGAAACTT CTATTGCAAC AGTTCAGGAG AAGCTAGCTG CACTTGAATC AAGTATTGAA 
CAGCGACTCA AGTGGGCAGG TGGTGCCAAC CCTGCATTGG CCCCTGTACT ACAAGATTTT 
GAAGCAACGA TAGCTGAAAG AAGAAATCTT GTCCTTAAAG AGAGCCAAAG AGCAAGTCAG 
GTCACATTTC TCTGCAGCAA TATCATTCAT TTTGAAAGTT TACGAACAAG AACTGCAGAA 
GCCTTAAACC TGGATGCGGC GTTATTTGAA CTAATCAAGC GATGTCAGCA GATGTGTTCG 
TTTGCATCAC AGTTTAACAG TTCAGTGTCT GAGTTAGAGC TTCGTTTATT ACAGAGAGTG 
GACACTGGTC TTGAACATCC TATTGGCAGC TCTGAATGGC TTTTGTCAGC ACACAAACAG 
TTGACCCAGG ATATGTCTAC TCAGAGGGCA ATTCAGACAG AGAAAGAGCA GCAGATAGAA 
ACGGTCTGTG AAACAATTCA GAATCTGGTT GATAATATAA AGACTGTGCT CACTGGTCAT 
AACCGACAGC TTGGAGATGT CAAACATCTC TTGAAAGCTA TGGCTAAGGA TGAAGAAGCT 
GCTCTGGCAG ATGGTGAAGA TGTTCCCTAT GAGAACAGTG TTAGGCAGTT TTTGGGTGAA 
TATAAATCAT GGCAAGACAA CATTCAAACA GTTCTATTTA CATTAGTCCA GGCTATGGGT 
CAGGTTCGAA GTCAAGAACA CGTTGAAATG CTCCAGGAAA TCACTCCCAC CTTGAAAGAA 
CTGAAAACAC AAAGTCAGAG TATCTATAAT AATTTAGTGA GTTTTGCATC ACCCTTAGTC 
ACCGATGCAA CAAATGAATG TTCGAGTCCA ACGTCATCTG CTACTTATCA GCCATCCTTC 
GCTGCAGCAG TCCGGAGTAA CACTGGCCAG AAGACTCAGC CTGATGTCAT GTCACAGAAT 
GCTAGAAAGC TGATCCAAAA AAATCTTGCT ACATCAGCTG ATACTCCACC AAGCACCGTT 
CCAGGAACTG GCAAGAGTGT TGCTTGTAGT CCTAAAAAGG CAGTCAGAGA CCCTAAAACT 
GGGAAAGCGG TGCAAGAGAG AAACTCCTAT GCAGTGAGTG TGTGGAAGAG AGTGAAAGCC 
AAGTTAGAGG GCCGAGATGT TGATCCGAAT AGGAGGATGT CAGTTGCTGA ACAGGTTGAC 
TATGTCATTA AGGAAGCAAC TAATCTAGAT AACTTGGCTC AGCTGTATGA AGGTTGGACA 
GCCTGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255240.1
CDS	513..11504
Translation	MRFLRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSVQHGSRALATKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGNIKVDFTFMRAKCFLVKGQKYNDLSFQGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_005255183.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255183.3

ORF Insert Sequence:

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATAATTTAAA ATATTCTTCA 
TCCAGAGATA GAGGTGGTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACGCTGACA TACAGAATGA CGAAAAGGGT 
GGCTACAGTG TCAATGGAGG ATCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAACAATGT GACCAGCCCT GAGTTCACCA GTGTTCAGCA TGGCAGTCGT 
GCTTTAGCCA CCAAAGACAT GAGGAAATCA CAGGAGAGAT CGATGTCTTA TTCTGATGAG 
TCTCGACTGT CGAATCTTCT TCGGAGGATC ACCCGGGAAG ACGACAGAGA CCGAAGATTG 
GCTACTGTAA AGCAGTTGAA AGAATTTATT CAGCAACCAG AAAATAAGCT GGTACTAGTT 
AAACAATTGG ATAATATCTT GGCTGCTGTA CATGACGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAGT TGAGACAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTCTGAGC 
TATGAGGCTG AGAAGATCTT CAAGTGGATT TTTAGCAAAT TTAGCTCATC TGCAAAAGAT 
GAAGTTAAAC TCCTCTACTT ATGTGCCACC TACAAAGCAC TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCTGTAAT GCAGCTTGTA ATGACCAGCC TGCAGTCTAT TCTTGAAAAT 
GTGGATACAC CAGAATTGCT TTGTAAATGT GTTAAGTGCA TTCTTTTGGT GGCTCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACAGTTG ATATATTAGT TGGATGGCAT 
ATAGATCATA CTCAGAAACC TTCGCTCACG CAGCAGGTAT CTGGGTGGTT GCAGAGTTTG 
GAGCCATTTT GGGTAGCTGA TCTTGCATTT TCTACTACTC TTCTTGGTCA GTTTCTGGAA 
GACATGGAAG CATATGCTGA GGACCTCAGC CATGTGGCCT CTGGGGAATC AGTGGATGAA 
GATGTCCCTC CTCCATCAGT GTCATTACCA AAGCTGGCTG CACTTCTCCG GGTATTTAGT 
ACTGTGGTGA GGAGCATTGG GGAACGCTTC AGCCCAATTC GGGGTCCTCC AATTACTGAG 
GCATATGTAA CAGATGTTCT GTACAGAGTA ATGAGATGTG TGACGGCTGC AAACCAGGTG 
TTTTTTTCTG AGGCTGTGTT GACAGCTGCT AATGAGTGTG TTGGTGTTTT GCTCGGCAGC 
TTGGATCCTA GCATGACTAT ACATTGTGAC ATGGTCATTA CATATGGATT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACCGATTAT ATCATCTCAG TCTTGAATTT ACTCACGCTG 
ATTGTTGAAC AGATAAATAC GAAACTGCCA TCATCATTTG TAGAAAAACT GTTTATACCA 
TCATCTAAAC TACTATTCTT GCGTTATCAT AAAGAAAAAG AGGTTGTTGC TGTAGCCCAT 
GCTGTTTATC AAGCAGTGCT CAGCTTGAAG AATATTCCTG TTTTGGAGAC TGCCTATAAG 
TTAATATTGG GAGAAATGAC TTGTGCCCTA AACAACCTCC TACACAGTCT ACAACTTCCT 
GAGGCCTGTT CTGAAATAAA ACATGAGGCT TTTAAGAATC ATGTGTTCAA TGTAGACAAT 
GCAAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACTA CAATTGGAAA TGCCAAAAAC 
TCACTAATAG GGATGTGGGC GCTATCTCCA ACTGTCTTTG CACTTCTGAG TAAGAATCTG 
ATGATTGTGC ACAGTGACCT GGCTGTTCAC TTCCCTGCCA TTCAGTATGC TGTGCTCTAC 
ACATTGTATT CTCATTGTAC CAGGCATGAT CACTTTATCT CTAGTAGCCT CAGTTCTTCC 
TCTCCTTCTT TGTTTGATGG AGCTGTGATT AGCACTGTAA CTACGGCTAC AAAGAAACAT 
TTCTCAATTA TATTAAATCT TCTGGGAATA TTACTTAAGA AAGATAACCT TAACCAGGAC 
ACGAGGAAAC TGTTAATGAC TTGGGCTTTG GAAGCAGCTG TTTTAATGAA GAAGTCTGAA 
ACATACGCAC CTTTATTCTC TCTTCCGTCT TTCCATAAAT TTTGCAAAGG CCTTTTAGCC 
AACACTCTCG TTGAAGATGT GAATATCTGT CTGCAGGCAT GCAGCAGTCT ACATGCTCTG 
TCCTCTTCCT TGCCAGATGA TCTTTTACAG AGATGTGTCG ATGTTTGCCG TGTTCAACTA 
GTGCACAGTG GAACTCGTAT TCGACAAGCA TTTGGAAAAC TGTTGAAATC AATTCCTTTA 
GATGTTGTCC TAAGCAATAA CAATCACACA GAAATTCAAG AAATTTCTTT AGCATTAAGA 
AGTCACATGA GTAAAGCACC AAGTAATACA TTCCACCCCC AAGATTTCTC TGATGTTATT 
AGTTTTATTT TGTATGGGAA CTCTCATAGA ACAGGGAAGG ACAATTGGTT GGAAAGACTG 
TTCTATAGCT GCCAGAGACT GGATAAGCGT GACCAGTCAA CAATTCCACG CAATCTCCTG 
AAGACAGATG CTGTCCTTTG GCAGTGGGCC ATATGGGAAG CTGCACAATT CACTGTTCTT 
TCTAAGCTGA GAACCCCACT GGGCAGAGCT CAAGACACCT TCCAGACAAT TGAAGGTATC 
ATTCGAAGTC TCGCAGCTCA CACATTAAAC CCTGATCAGG ATGTTAGTCA GTGGACAACT 
GCAGACAATG ATGAAGGCCA TGGTAACAAC CAACTTAGAC TTGTTCTTCT TCTGCAGTAT 
CTGGAAAATC TGGAGAAATT AATGTATAAT GCATACGAGG GATGTGCTAA TGCATTAACT 
TCACCTCCCA AGGTCATTAG AACTTTTTTC TATACCAATC GCCAAACTTG TCAGGACTGG 
CTAACGCGGA TTCGACTCTC CATCATGAGG GTAGGATTGT TGGCAGGCCA GCCTGCAGTG 
ACAGTGAGAC ATGGCTTTGA CTTGCTTACA GAGATGAAAA CAACCAGCCT ATCTCAGGGG 
AATGAATTGG AAGTAACCAT TATGATGGTG GTAGAAGCAT TATGTGAACT TCATTGTCCT 
GAAGCTATAC AGGGAATTGC TGTCTGGTCA TCATCTATTG TTGGAAAAAA TCTTCTGTGG 
ATTAACTCAG TGGCTCAACA GGCTGAAGGG AGGTTTGAAA AGGCCTCTGT GGAGTACCAG 
GAACACCTGT GTGCCATGAC AGGTGTTGAT TGCTGCATCT CCAGCTTTGA CAAATCGGTG 
CTCACCTTAG CCAATGCTGG GCGTAACAGT GCCAGCCCGA AACATTCTCT GAATGGTGAA 
TCCAGAAAAA CTGTGCTGTC CAAACCGACT GACTCTTCCC CTGAGGTTAT AAATTATTTA 
GGAAATAAAG CATGTGAGTG CTACATCTCA ATTGCCGATT GGGCTGCTGT GCAGGAATGG 
CAGAACGCTA TCCATGACTT GAAAAAGAGT ACCAGTAGCA CTTCCCTCAA CCTGAAAGCT 
GACTTCAACT ATATAAAATC ATTAAGCAGC TTTGAGTCTG GAAAATTTGT TGAATGTACC 
GAGCAGTTAG AATTGTTACC AGGAGAAAAT ATCAATCTAC TTGCTGGAGG ATCAAAAGAA 
AAAATAGACA TGAAAAAACT GCTTCCTAAC ATGTTAAGTC CGGATCCGAG GGAACTTCAG 
AAATCCATTG AAGTTCAATT GTTAAGAAGT TCTGTTTGTT TGGCAACTGC TTTAAACCCG 
ATAGAACAAG ATCAGAAGTG GCAGTCTATA ACTGAAAATG TGGTAAAGTA CTTGAAGCAA 
ACATCCCGCA TCGCTATTGG ACCTCTGAGA CTTTCTACTT TAACAGTTTC ACAGTCTTTG 
CCAGTTCTAA GTACCTTGCA GCTGTATTGC TCATCTGCTT TGGAGAACAC AGTTTCTAAC 
AGACTTTCAA CAGAGGACTG TCTTATTCCA CTCTTCAGTG AAGCTTTACG TTCATGTAAA 
CAGCATGACG TGAGGCCATG GATGCAGGCA TTAAGGTATA CTATGTACCA GAATCAGTTG 
TTGGAGAAAA TTAAAGAACA AACAGTCCCA ATTAGAAGCC ATCTCATGGA ATTAGGTCTA 
ACAGCAGCAA AATTTGCTAG AAAACGAGGG AATGTGTCCC TTGCAACAAG ACTGCTGGCA 
CAGTGCAGTG AAGTTCAGCT GGGAAAGACC ACCACTGCAC AGGATTTAGT CCAACATTTT 
AAAAAACTAT CAACCCAAGG TCAAGTGGAT GAAAAATGGG GGCCCGAACT TGATATTGAA 
AAAACCAAAT TGCTTTATAC AGCAGGCCAG TCAACACATG CAATGGAAAT GTTGAGTTCT 
TGTGCCATAT CTTTCTGCAA GTCTGTGAAA GCTGAATATG CAGTTGCTAA ATCAATTCTG 
ACACTGGCTA AATGGATCCA GGCAGAATGG AAAGAGATTT CAGGACAGCT GAAACAGGTT 
TACAGAGCTC AGCACCAACA GAACTTCACA GGTCTTTCTA CTTTGTCTAA AAACATACTC 
ACTCTAATAG AACTGCCATC TGTTAATACG ATGGAAGAAG AGTATCCTCG GATCGAGAGT 
GAATCTACAG TGCATATTGG AGTTGGAGAA CCTGACTTCA TTTTGGGACA GTTGTATCAC 
CTGTCTTCAG TACAGGCACC TGAAGTAGCC AAATCTTGGG CAGCGTTGGC CAGCTGGGCT 
TATAGGTGGG GCAGAAAGGT GGTTGACAAT GCCAGTCAGG GAGAAGGTGT TCGTCTGCTG 
CCTAGAGAAA AATCTGAAGT TCAGAATCTA CTTCCAGACA CTATAACTGA GGAAGAGAAA 
GAGAGAATAT ATGGTATTCT TGGACAGGCT GTGTGTCGGC CGGCGGGGAT TCAGGATGAA 
GATATAACAC TTCAGATAAC TGAGAGTGAA GACAACGAAG AAGATGACAT GGTTGATGTT 
ATCTGGCGTC AGTTGATATC AAGCTGCCCA TGGCTTTCAG AACTTGATGA AAGTGCAACT 
GAAGGAGTTA TTAAAGTGTG GAGGAAAGTT GTAGATAGAA TATTCAGCCT GTACAAACTC 
TCTTGCAGTG CATACTTTAC TTTCCTTAAA CTCAACGCTG GTCAAATTCC TTTAGATGAG 
GATGACCCTA GGCTGCATTT AAGTCACAGA GTGGAACAGA GCACTGATGA CATGATTGTG 
ATGGCCACAT TGCGCCTGCT GCGGTTGCTC GTGAAGCATG CTGGTGAGCT TCGGCAGTAT 
CTGGAGCACG GCTTGGAGAC AACACCCACT GCACCATGGA GAGGAATTAT TCCGCAACTT 
TTCTCACGCT TAAACCACCC TGAAGTGTAT GTGCGCCAAA GTATTTGTAA CCTTCTCTGC 
CGTGTGGCTC AAGATTCCCC ACATCTCATA TTGTATCCTG CAATAGTGGG TACCATATCG 
CTTAGTAGTG AATCCCAGGC TTCAGGAAAT AAATTTTCCA CTGCAATTCC AACTTTACTT 
GGCAATATTC AAGGAGAAGA ATTGCTGGTT TCTGAATGTG AGGGAGGAAG TCCTCCTGCA 
TCTCAGGATA GCAATAAGGA TGAACCTAAA AGTGGATTAA ATGAAGACCA AGCCATGATG 
CAGGATTGTT ACAGCAAAAT TGTAGATAAG CTGTCCTCTG CAAACCCCAC CATGGTATTA 
CAGGTTCAGA TGCTCGTGGC TGAACTGCGC AGGGTCACTG TGCTCTGGGA TGAGCTCTGG 
CTGGGAGTTT TGCTGCAACA ACACATGTAT GTCCTGAGAC GAATTCAGCA GCTTGAAGAT 
GAGGTGAAGA GAGTCCAGAA CAACAACACC TTACGCAAAG AAGAGAAAAT TGCAATCATG 
AGGGAGAAGC ACACAGCTTT GATGAAGCCC ATCGTATTTG CTTTGGAGCA TGTGAGGAGT 
ATCACAGCGG CTCCTGCAGA AACACCTCAT GAAAAATGGT TTCAGGATAA CTATGGTGAT 
GCCATTGAAA ATGCCCTAGA AAAACTGAAG ACTCCATTGA ACCCTGCAAA GCCTGGGAGC 
AGCTGGATTC CATTTAAAGA GATAATGCTA AGTTTGCAAC AGAGAGCACA GAAACGTGCA 
AGTTACATCT TGCGTCTTGA AGAAATCAGT CCATGGTTGG CTGCCATGAC TAACACTGAA 
ATTGCTCTTC CTGGGGAAGT CTCAGCCAGA GACACTGTCA CAATCCATAG TGTGGGCGGA 
ACCATCACAA TCTTACCGAC TAAAACCAAG CCAAAGAAAC TTCTCTTTCT TGGATCAGAT 
GGGAAGAGCT ATCCTTATCT TTTCAAAGGA CTGGAGGATT TACATCTGGA TGAGAGAATA 
ATGCAGTTCC TATCTATTGT GAATACCATG TTTGCTACAA TTAATCGCCA AGAAACACCC 
CGGTTCCATG CTCGACACTA TTCTGTAACA CCACTAGGAA CAAGATCAGG ACTAATCCAG 
TGGGTAGATG GAGCCACACC CTTATTTGGT CTTTACAAAC GATGGCAACA ACGGGAAGCT 
GCCTTACAAG CACAAAAGGC CCAAGATTCC TACCAAACTC CTCAGAATCC TGGAATTGTA 
CCCCGTCCTA GTGAACTTTA TTACAGTAAA ATTGGCCCTG CTTTGAAAAC AGTTGGGCTT 
AGCCTGGATG TGTCCCGTCG GGATTGGCCT CTTCATGTAA TGAAGGCAGT ATTGGAAGAG 
TTAATGGAGG CCACACCCCC GAATCTCCTT GCCAAAGAGC TCTGGTCATC TTGCACAACA 
CCTGATGAAT GGTGGAGAGT TACGCAGTCT TATGCAAGAT CTACTGCAGT CATGTCTATG 
GTTGGATACA TAATTGGCCT TGGAGACAGA CATCTGGATA ATGTTCTTAT AGATATGACG 
ACTGGAGAAG TTGTTCACAT AGATTACAAT GTTTGCTTTG AAAAAGGTAA TATAAAAGTA 
GACTTTACAT TTATGAGGGC CAAATGTTTT TTGGTGAAAG GGCAAAAATA TAATGATTTG 
TCTTTTCAAG GTAAAAGCCT TAGAGTTCCT GAGAAAGTAC CTTTTCGAAT GACACAAAAC 
ATTGAAACAG CACTGGGTGT AACTGGAGTA GAAGGTGTAT TTAGGCTTTC ATGTGAGCAG 
GTTTTACACA TTATGCGGCG TGGCAGAGAG ACCCTGCTGA CGCTGCTGGA GGCCTTTGTG 
TACGACCCTC TGGTGGACTG GACAGCAGGA GGCGAGGCTG GGTTTGCTGG TGCTGTCTAT 
GGTGGAGGTG GCCAGCAGGC CGAGAGCAAG CAGAGCAAGA GAGAGATGGA GCGAGAGATC 
ACCCGCAGCC TGTTTTCTTC TAGAGTAGCT GAGATTAAGG TGAACTGGTT TAAGAATAGA 
GATGAGATGC TGGTTGTGCT TCCCAAGTTG GACGGTAGCT TAGATGAATA CCTAAGCTTG 
CAAGAGCAAC TGACAGATGT GGAAAAACTG CAGGGCAAAC TACTGGAGGA AATAGAGTTT 
CTAGAAGGAG CTGAAGGGGT GGATCATCCT TCTCATACTC TGCAACACAG GTATTCTGAG 
CACACCCAAC TACAGACTCA GCAAAGAGCT GTTCAGGAAG CAATCCAGGT GAAGCTGAAT 
GAATTTGAAC AATGGATAAC ACATTATCAG GCTGCATTCA ATAATTTAGA AGCAACACAG 
CTTGCAAGCT TGCTTCAAGA GATAAGCACA CAAATGGACC TTGGTCCTCC AAGTTACGTG 
CCAGCAACAG CCTTTCTGCA GAATGCTGGT CAGGCCCACT TGATTAGCCA GTGCGAGCAG 
CTGGAGGGGG AGGTTGGTGC TCTCCTGCAG CAGAGGCGCT CCGTGCTCCG TGGCTGTCTG 
GAGCAACTGC ATCACTATGC AACCGTGGCC CTGCAGTATC CGAAGGCCAT ATTTCAGAAA 
CATCGAATTG AACAGTGGAA GACCTGGATG GAAGAGCTCA TCTGTAACAC CACAGTAGAG 
CGTTGTCAAG AGCTCTATAG GAAATATGAA ATGCAATATG CTCCCCAGCC ACCCCCAACA 
GTGTGTCAGT TCATCACTGC CACTGAAATG ACCCTGCAGC GATACGCAGC AGACATCAAC 
AGCAGACTTA TTAGACAAGT GGAACGCTTG AAACAGGAAG CTGTCACTGT GCCAGTTTGT 
GAAGATCAGT TGAAAGAAAT TGAACGTTGC ATTAAAGTTT TCCTTCATGA GAATGGAGAA 
GAAGGATCTT TGAGTCTAGC AAGTGTTATT ATTTCTGCCC TTTGTACCCT TACAAGGCGT 
AACCTGATGA TGGAAGGTGC AGCGTCAAGT GCTGGAGAAC AGCTGGTTGA TCTGACTTCT 
CGGGATGGAG CCTGGTTCTT GGAGGAACTC TGCAGTATGA GCGGAAACGT CACCTGCTTG 
GTTCAGTTAC TGAAGCAGTG CCACCTGGTG CCACAGGACT TAGATATCCC GAACCCCATG 
GAAGCGTCTG AGACAGTTCA CTTAGCCAAT GGAGTGTATA CCTCACTTCA GGAATTGAAT 
TCGAATTTCC GGCAAATCAT ATTTCCAGAA GCACTTCGAT GTTTAATGAA AGGGGAATAC 
ACGTTAGAAA GTATGCTGCA TGAACTGGAC GGTCTTATTG AGCAGACCAC CGATGGCGTT 
CCCCTGCAGA CTCTAGTGGA ATCTCTTCAG GCCTACTTAA GAAACGCAGC TATGGGACTG 
GAAGAAGAAA CACATGCTCA TTACATCGAT GTTGCCAGAC TACTACATGC TCAGTACGGT 
GAATTAATCC AACCGAGAAA TGGTTCAGTT GATGAAACAC CCAAAATGTC AGCTGGCCAG 
ATGCTTTTGG TAGCATTCGA TGGCATGTTT GCTCAAGTTG AAACTGCTTT CAGCTTATTA 
GTTGAAAAGT TGAACAAGAT GGAAATTCCC ATAGCTTGGC GAAAGATTGA CATCATAAGG 
GAAGCCAGGA GTACTCAAGT TAATTTTTTT GATGATGATA ATCACCGGCA GGTGCTAGAA 
GAGATTTTCT TTCTAAAAAG ACTACAGACT ATTAAGGAGT TCTTCAGGCT CTGTGGTACC 
TTTTCTAAAA CATTGTCAGG ATCAAGTTCA CTTGAAGATC AGAATACTGT GAATGGGCCT 
GTACAGATTG TCAATGTGAA AACCCTTTTT AGAAACTCTT GTTTCAGTGA AGACCAAATG 
GCCAAACCTA TCAAGGCATT CACAGCTGAC TTTGTGAGGC AGCTCTTGAT AGGGCTACCC 
AACCAAGCCC TCGGACTCAC ACTGTGCAGT TTTATCAGTG CTCTGGGTGT AGACATCATT 
GCTCAAGTAG AGGCAAAGGA CTTTGGTGCC GAAAGCAAAG TTTCTGTTGA TGATCTCTGT 
AAGAAAGCGG TGGAACATAA CATCCAGATA GGGAAGTTCT CTCAGCTGGT TATGAACAGG 
GCAACTGTGT TAGCAAGTTC TTACGACACT GCCTGGAAGA AGCATGACTT GGTGCGAAGG 
CTAGAAACCA GTATTTCTTC TTGTAAGACA AGCCTGCAGC GGGTTCAGCT GCATATTGCC 
ATGTTTCAGT GGCAACATGA AGATCTACTT ATCAATAGAC CACAAGCCAT GTCAGTCACA 
CCTCCCCCAC GGTCTGCTAT CCTAACCAGC ATGAAAAAGA AGCTGCATAC CCTGAGCCAG 
ATTGAAACTT CTATTGCAAC AGTTCAGGAG AAGCTAGCTG CACTTGAATC AAGTATTGAA 
CAGCGACTCA AGTGGGCAGG TGGTGCCAAC CCTGCATTGG CCCCTGTACT ACAAGATTTT 
GAAGCAACGA TAGCTGAAAG AAGAAATCTT GTCCTTAAAG AGAGCCAAAG AGCAAGTCAG 
GTCACATTTC TCTGCAGCAA TATCATTCAT TTTGAAAGTT TACGAACAAG AACTGCAGAA 
GCCTTAAACC TGGATGCGGC GTTATTTGAA CTAATCAAGC GATGTCAGCA GATGTGTTCG 
TTTGCATCAC AGTTTAACAG TTCAGTGTCT GAGTTAGAGC TTCGTTTATT ACAGAGAGTG 
GACACTGGTC TTGAACATCC TATTGGCAGC TCTGAATGGC TTTTGTCAGC ACACAAACAG 
TTGACCCAGG ATATGTCTAC TCAGAGGGCA ATTCAGACAG AGAAAGAGCA GCAGATAGAA 
ACGGTCTGTG AAACAATTCA GAATCTGGTT GATAATATAA AGACTGTGCT CACTGGTCAT 
AACCGACAGC TTGGAGATGT CAAACATCTC TTGAAAGCTA TGGCTAAGGA TGAAGAAGCT 
GCTCTGGCAG ATGGTGAAGA TGTTCCCTAT GAGAACAGTG TTAGGCAGTT TTTGGGTGAA 
TATAAATCAT GGCAAGACAA CATTCAAACA GTTCTATTTA CATTAGTCCA GGCTATGGGT 
CAGGTTCGAA GTCAAGAACA CGTTGAAATG CTCCAGGAAA TCACTCCCAC CTTGAAAGAA 
CTGAAAACAC AAAGTCAGAG TATCTATAAT AATTTAGTGA GTTTTGCATC ACCCTTAGTC 
ACCGATGCAA CAAATGAATG TTCGAGTCCA ACGTCATCTG CTACTTATCA GCCATCCTTC 
GCTGCAGCAG TCCGGAGTAA CACTGGCCAG AAGACTCAGC CTGATGTCAT GTCACAGAAT 
GCTAGAAAGC TGATCCAAAA AAATCTTGCT ACATCAGCTG ATACTCCACC AAGCACCGTT 
CCAGGAACTG GCAAGAGTGT TGCTTGTAGT CCTAAAAAGG CAGTCAGAGA CCCTAAAACT 
GGGAAAGCGG TGCAAGAGAG AAACTCCTAT GCAGTGAGTG TGTGGAAGAG AGTGAAAGCC 
AAGTTAGAGG GCCGAGATGT TGATCCGAAT AGGAGGATGT CAGTTGCTGA ACAGGTTGAC 
TATGTCATTA AGGAAGCAAC TAATCTAGAT AACTTGGCTC AGCTGTATGA AGGTTGGACA 
GCCTGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_017023066.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878555.1 Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X4, mRNA.

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CloneID	OHu49089
Clone ID Related Accession (Same CDS sequence)	XM_017023066.1 , XM_005255183.4 , XM_005255183.3 , XM_017023066.2
Accession Version	XM_017023066.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023066.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATAATTTAAA ATATTCTTCA 
TCCAGAGATA GAGGTGGTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACGCTGACA TACAGAATGA CGAAAAGGGT 
GGCTACAGTG TCAATGGAGG ATCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAACAATGT GACCAGCCCT GAGTTCACCA GTGTTCAGCA TGGCAGTCGT 
GCTTTAGCCA CCAAAGACAT GAGGAAATCA CAGGAGAGAT CGATGTCTTA TTCTGATGAG 
TCTCGACTGT CGAATCTTCT TCGGAGGATC ACCCGGGAAG ACGACAGAGA CCGAAGATTG 
GCTACTGTAA AGCAGTTGAA AGAATTTATT CAGCAACCAG AAAATAAGCT GGTACTAGTT 
AAACAATTGG ATAATATCTT GGCTGCTGTA CATGACGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAGT TGAGACAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTCTGAGC 
TATGAGGCTG AGAAGATCTT CAAGTGGATT TTTAGCAAAT TTAGCTCATC TGCAAAAGAT 
GAAGTTAAAC TCCTCTACTT ATGTGCCACC TACAAAGCAC TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCTGTAAT GCAGCTTGTA ATGACCAGCC TGCAGTCTAT TCTTGAAAAT 
GTGGATACAC CAGAATTGCT TTGTAAATGT GTTAAGTGCA TTCTTTTGGT GGCTCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACAGTTG ATATATTAGT TGGATGGCAT 
ATAGATCATA CTCAGAAACC TTCGCTCACG CAGCAGGTAT CTGGGTGGTT GCAGAGTTTG 
GAGCCATTTT GGGTAGCTGA TCTTGCATTT TCTACTACTC TTCTTGGTCA GTTTCTGGAA 
GACATGGAAG CATATGCTGA GGACCTCAGC CATGTGGCCT CTGGGGAATC AGTGGATGAA 
GATGTCCCTC CTCCATCAGT GTCATTACCA AAGCTGGCTG CACTTCTCCG GGTATTTAGT 
ACTGTGGTGA GGAGCATTGG GGAACGCTTC AGCCCAATTC GGGGTCCTCC AATTACTGAG 
GCATATGTAA CAGATGTTCT GTACAGAGTA ATGAGATGTG TGACGGCTGC AAACCAGGTG 
TTTTTTTCTG AGGCTGTGTT GACAGCTGCT AATGAGTGTG TTGGTGTTTT GCTCGGCAGC 
TTGGATCCTA GCATGACTAT ACATTGTGAC ATGGTCATTA CATATGGATT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACCGATTAT ATCATCTCAG TCTTGAATTT ACTCACGCTG 
ATTGTTGAAC AGATAAATAC GAAACTGCCA TCATCATTTG TAGAAAAACT GTTTATACCA 
TCATCTAAAC TACTATTCTT GCGTTATCAT AAAGAAAAAG AGGTTGTTGC TGTAGCCCAT 
GCTGTTTATC AAGCAGTGCT CAGCTTGAAG AATATTCCTG TTTTGGAGAC TGCCTATAAG 
TTAATATTGG GAGAAATGAC TTGTGCCCTA AACAACCTCC TACACAGTCT ACAACTTCCT 
GAGGCCTGTT CTGAAATAAA ACATGAGGCT TTTAAGAATC ATGTGTTCAA TGTAGACAAT 
GCAAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACTA CAATTGGAAA TGCCAAAAAC 
TCACTAATAG GGATGTGGGC GCTATCTCCA ACTGTCTTTG CACTTCTGAG TAAGAATCTG 
ATGATTGTGC ACAGTGACCT GGCTGTTCAC TTCCCTGCCA TTCAGTATGC TGTGCTCTAC 
ACATTGTATT CTCATTGTAC CAGGCATGAT CACTTTATCT CTAGTAGCCT CAGTTCTTCC 
TCTCCTTCTT TGTTTGATGG AGCTGTGATT AGCACTGTAA CTACGGCTAC AAAGAAACAT 
TTCTCAATTA TATTAAATCT TCTGGGAATA TTACTTAAGA AAGATAACCT TAACCAGGAC 
ACGAGGAAAC TGTTAATGAC TTGGGCTTTG GAAGCAGCTG TTTTAATGAA GAAGTCTGAA 
ACATACGCAC CTTTATTCTC TCTTCCGTCT TTCCATAAAT TTTGCAAAGG CCTTTTAGCC 
AACACTCTCG TTGAAGATGT GAATATCTGT CTGCAGGCAT GCAGCAGTCT ACATGCTCTG 
TCCTCTTCCT TGCCAGATGA TCTTTTACAG AGATGTGTCG ATGTTTGCCG TGTTCAACTA 
GTGCACAGTG GAACTCGTAT TCGACAAGCA TTTGGAAAAC TGTTGAAATC AATTCCTTTA 
GATGTTGTCC TAAGCAATAA CAATCACACA GAAATTCAAG AAATTTCTTT AGCATTAAGA 
AGTCACATGA GTAAAGCACC AAGTAATACA TTCCACCCCC AAGATTTCTC TGATGTTATT 
AGTTTTATTT TGTATGGGAA CTCTCATAGA ACAGGGAAGG ACAATTGGTT GGAAAGACTG 
TTCTATAGCT GCCAGAGACT GGATAAGCGT GACCAGTCAA CAATTCCACG CAATCTCCTG 
AAGACAGATG CTGTCCTTTG GCAGTGGGCC ATATGGGAAG CTGCACAATT CACTGTTCTT 
TCTAAGCTGA GAACCCCACT GGGCAGAGCT CAAGACACCT TCCAGACAAT TGAAGGTATC 
ATTCGAAGTC TCGCAGCTCA CACATTAAAC CCTGATCAGG ATGTTAGTCA GTGGACAACT 
GCAGACAATG ATGAAGGCCA TGGTAACAAC CAACTTAGAC TTGTTCTTCT TCTGCAGTAT 
CTGGAAAATC TGGAGAAATT AATGTATAAT GCATACGAGG GATGTGCTAA TGCATTAACT 
TCACCTCCCA AGGTCATTAG AACTTTTTTC TATACCAATC GCCAAACTTG TCAGGACTGG 
CTAACGCGGA TTCGACTCTC CATCATGAGG GTAGGATTGT TGGCAGGCCA GCCTGCAGTG 
ACAGTGAGAC ATGGCTTTGA CTTGCTTACA GAGATGAAAA CAACCAGCCT ATCTCAGGGG 
AATGAATTGG AAGTAACCAT TATGATGGTG GTAGAAGCAT TATGTGAACT TCATTGTCCT 
GAAGCTATAC AGGGAATTGC TGTCTGGTCA TCATCTATTG TTGGAAAAAA TCTTCTGTGG 
ATTAACTCAG TGGCTCAACA GGCTGAAGGG AGGTTTGAAA AGGCCTCTGT GGAGTACCAG 
GAACACCTGT GTGCCATGAC AGGTGTTGAT TGCTGCATCT CCAGCTTTGA CAAATCGGTG 
CTCACCTTAG CCAATGCTGG GCGTAACAGT GCCAGCCCGA AACATTCTCT GAATGGTGAA 
TCCAGAAAAA CTGTGCTGTC CAAACCGACT GACTCTTCCC CTGAGGTTAT AAATTATTTA 
GGAAATAAAG CATGTGAGTG CTACATCTCA ATTGCCGATT GGGCTGCTGT GCAGGAATGG 
CAGAACGCTA TCCATGACTT GAAAAAGAGT ACCAGTAGCA CTTCCCTCAA CCTGAAAGCT 
GACTTCAACT ATATAAAATC ATTAAGCAGC TTTGAGTCTG GAAAATTTGT TGAATGTACC 
GAGCAGTTAG AATTGTTACC AGGAGAAAAT ATCAATCTAC TTGCTGGAGG ATCAAAAGAA 
AAAATAGACA TGAAAAAACT GCTTCCTAAC ATGTTAAGTC CGGATCCGAG GGAACTTCAG 
AAATCCATTG AAGTTCAATT GTTAAGAAGT TCTGTTTGTT TGGCAACTGC TTTAAACCCG 
ATAGAACAAG ATCAGAAGTG GCAGTCTATA ACTGAAAATG TGGTAAAGTA CTTGAAGCAA 
ACATCCCGCA TCGCTATTGG ACCTCTGAGA CTTTCTACTT TAACAGTTTC ACAGTCTTTG 
CCAGTTCTAA GTACCTTGCA GCTGTATTGC TCATCTGCTT TGGAGAACAC AGTTTCTAAC 
AGACTTTCAA CAGAGGACTG TCTTATTCCA CTCTTCAGTG AAGCTTTACG TTCATGTAAA 
CAGCATGACG TGAGGCCATG GATGCAGGCA TTAAGGTATA CTATGTACCA GAATCAGTTG 
TTGGAGAAAA TTAAAGAACA AACAGTCCCA ATTAGAAGCC ATCTCATGGA ATTAGGTCTA 
ACAGCAGCAA AATTTGCTAG AAAACGAGGG AATGTGTCCC TTGCAACAAG ACTGCTGGCA 
CAGTGCAGTG AAGTTCAGCT GGGAAAGACC ACCACTGCAC AGGATTTAGT CCAACATTTT 
AAAAAACTAT CAACCCAAGG TCAAGTGGAT GAAAAATGGG GGCCCGAACT TGATATTGAA 
AAAACCAAAT TGCTTTATAC AGCAGGCCAG TCAACACATG CAATGGAAAT GTTGAGTTCT 
TGTGCCATAT CTTTCTGCAA GTCTGTGAAA GCTGAATATG CAGTTGCTAA ATCAATTCTG 
ACACTGGCTA AATGGATCCA GGCAGAATGG AAAGAGATTT CAGGACAGCT GAAACAGGTT 
TACAGAGCTC AGCACCAACA GAACTTCACA GGTCTTTCTA CTTTGTCTAA AAACATACTC 
ACTCTAATAG AACTGCCATC TGTTAATACG ATGGAAGAAG AGTATCCTCG GATCGAGAGT 
GAATCTACAG TGCATATTGG AGTTGGAGAA CCTGACTTCA TTTTGGGACA GTTGTATCAC 
CTGTCTTCAG TACAGGCACC TGAAGTAGCC AAATCTTGGG CAGCGTTGGC CAGCTGGGCT 
TATAGGTGGG GCAGAAAGGT GGTTGACAAT GCCAGTCAGG GAGAAGGTGT TCGTCTGCTG 
CCTAGAGAAA AATCTGAAGT TCAGAATCTA CTTCCAGACA CTATAACTGA GGAAGAGAAA 
GAGAGAATAT ATGGTATTCT TGGACAGGCT GTGTGTCGGC CGGCGGGGAT TCAGGATGAA 
GATATAACAC TTCAGATAAC TGAGAGTGAA GACAACGAAG AAGATGACAT GGTTGATGTT 
ATCTGGCGTC AGTTGATATC AAGCTGCCCA TGGCTTTCAG AACTTGATGA AAGTGCAACT 
GAAGGAGTTA TTAAAGTGTG GAGGAAAGTT GTAGATAGAA TATTCAGCCT GTACAAACTC 
TCTTGCAGTG CATACTTTAC TTTCCTTAAA CTCAACGCTG GTCAAATTCC TTTAGATGAG 
GATGACCCTA GGCTGCATTT AAGTCACAGA GTGGAACAGA GCACTGATGA CATGATTGTG 
ATGGCCACAT TGCGCCTGCT GCGGTTGCTC GTGAAGCATG CTGGTGAGCT TCGGCAGTAT 
CTGGAGCACG GCTTGGAGAC AACACCCACT GCACCATGGA GAGGAATTAT TCCGCAACTT 
TTCTCACGCT TAAACCACCC TGAAGTGTAT GTGCGCCAAA GTATTTGTAA CCTTCTCTGC 
CGTGTGGCTC AAGATTCCCC ACATCTCATA TTGTATCCTG CAATAGTGGG TACCATATCG 
CTTAGTAGTG AATCCCAGGC TTCAGGAAAT AAATTTTCCA CTGCAATTCC AACTTTACTT 
GGCAATATTC AAGGAGAAGA ATTGCTGGTT TCTGAATGTG AGGGAGGAAG TCCTCCTGCA 
TCTCAGGATA GCAATAAGGA TGAACCTAAA AGTGGATTAA ATGAAGACCA AGCCATGATG 
CAGGATTGTT ACAGCAAAAT TGTAGATAAG CTGTCCTCTG CAAACCCCAC CATGGTATTA 
CAGGTTCAGA TGCTCGTGGC TGAACTGCGC AGGGTCACTG TGCTCTGGGA TGAGCTCTGG 
CTGGGAGTTT TGCTGCAACA ACACATGTAT GTCCTGAGAC GAATTCAGCA GCTTGAAGAT 
GAGGTGAAGA GAGTCCAGAA CAACAACACC TTACGCAAAG AAGAGAAAAT TGCAATCATG 
AGGGAGAAGC ACACAGCTTT GATGAAGCCC ATCGTATTTG CTTTGGAGCA TGTGAGGAGT 
ATCACAGCGG CTCCTGCAGA AACACCTCAT GAAAAATGGT TTCAGGATAA CTATGGTGAT 
GCCATTGAAA ATGCCCTAGA AAAACTGAAG ACTCCATTGA ACCCTGCAAA GCCTGGGAGC 
AGCTGGATTC CATTTAAAGA GATAATGCTA AGTTTGCAAC AGAGAGCACA GAAACGTGCA 
AGTTACATCT TGCGTCTTGA AGAAATCAGT CCATGGTTGG CTGCCATGAC TAACACTGAA 
ATTGCTCTTC CTGGGGAAGT CTCAGCCAGA GACACTGTCA CAATCCATAG TGTGGGCGGA 
ACCATCACAA TCTTACCGAC TAAAACCAAG CCAAAGAAAC TTCTCTTTCT TGGATCAGAT 
GGGAAGAGCT ATCCTTATCT TTTCAAAGGA CTGGAGGATT TACATCTGGA TGAGAGAATA 
ATGCAGTTCC TATCTATTGT GAATACCATG TTTGCTACAA TTAATCGCCA AGAAACACCC 
CGGTTCCATG CTCGACACTA TTCTGTAACA CCACTAGGAA CAAGATCAGG ACTAATCCAG 
TGGGTAGATG GAGCCACACC CTTATTTGGT CTTTACAAAC GATGGCAACA ACGGGAAGCT 
GCCTTACAAG CACAAAAGGC CCAAGATTCC TACCAAACTC CTCAGAATCC TGGAATTGTA 
CCCCGTCCTA GTGAACTTTA TTACAGTAAA ATTGGCCCTG CTTTGAAAAC AGTTGGGCTT 
AGCCTGGATG TGTCCCGTCG GGATTGGCCT CTTCATGTAA TGAAGGCAGT ATTGGAAGAG 
TTAATGGAGG CCACACCCCC GAATCTCCTT GCCAAAGAGC TCTGGTCATC TTGCACAACA 
CCTGATGAAT GGTGGAGAGT TACGCAGTCT TATGCAAGAT CTACTGCAGT CATGTCTATG 
GTTGGATACA TAATTGGCCT TGGAGACAGA CATCTGGATA ATGTTCTTAT AGATATGACG 
ACTGGAGAAG TTGTTCACAT AGATTACAAT GTTTGCTTTG AAAAAGGTAA TATAAAAGTA 
GACTTTACAT TTATGAGGGC CAAATGTTTT TTGGTGAAAG GGCAAAAATA TAATGATTTG 
TCTTTTCAAG GTAAAAGCCT TAGAGTTCCT GAGAAAGTAC CTTTTCGAAT GACACAAAAC 
ATTGAAACAG CACTGGGTGT AACTGGAGTA GAAGGTGTAT TTAGGCTTTC ATGTGAGCAG 
GTTTTACACA TTATGCGGCG TGGCAGAGAG ACCCTGCTGA CGCTGCTGGA GGCCTTTGTG 
TACGACCCTC TGGTGGACTG GACAGCAGGA GGCGAGGCTG GGTTTGCTGG TGCTGTCTAT 
GGTGGAGGTG GCCAGCAGGC CGAGAGCAAG CAGAGCAAGA GAGAGATGGA GCGAGAGATC 
ACCCGCAGCC TGTTTTCTTC TAGAGTAGCT GAGATTAAGG TGAACTGGTT TAAGAATAGA 
GATGAGATGC TGGTTGTGCT TCCCAAGTTG GACGGTAGCT TAGATGAATA CCTAAGCTTG 
CAAGAGCAAC TGACAGATGT GGAAAAACTG CAGGGCAAAC TACTGGAGGA AATAGAGTTT 
CTAGAAGGAG CTGAAGGGGT GGATCATCCT TCTCATACTC TGCAACACAG GTATTCTGAG 
CACACCCAAC TACAGACTCA GCAAAGAGCT GTTCAGGAAG CAATCCAGGT GAAGCTGAAT 
GAATTTGAAC AATGGATAAC ACATTATCAG GCTGCATTCA ATAATTTAGA AGCAACACAG 
CTTGCAAGCT TGCTTCAAGA GATAAGCACA CAAATGGACC TTGGTCCTCC AAGTTACGTG 
CCAGCAACAG CCTTTCTGCA GAATGCTGGT CAGGCCCACT TGATTAGCCA GTGCGAGCAG 
CTGGAGGGGG AGGTTGGTGC TCTCCTGCAG CAGAGGCGCT CCGTGCTCCG TGGCTGTCTG 
GAGCAACTGC ATCACTATGC AACCGTGGCC CTGCAGTATC CGAAGGCCAT ATTTCAGAAA 
CATCGAATTG AACAGTGGAA GACCTGGATG GAAGAGCTCA TCTGTAACAC CACAGTAGAG 
CGTTGTCAAG AGCTCTATAG GAAATATGAA ATGCAATATG CTCCCCAGCC ACCCCCAACA 
GTGTGTCAGT TCATCACTGC CACTGAAATG ACCCTGCAGC GATACGCAGC AGACATCAAC 
AGCAGACTTA TTAGACAAGT GGAACGCTTG AAACAGGAAG CTGTCACTGT GCCAGTTTGT 
GAAGATCAGT TGAAAGAAAT TGAACGTTGC ATTAAAGTTT TCCTTCATGA GAATGGAGAA 
GAAGGATCTT TGAGTCTAGC AAGTGTTATT ATTTCTGCCC TTTGTACCCT TACAAGGCGT 
AACCTGATGA TGGAAGGTGC AGCGTCAAGT GCTGGAGAAC AGCTGGTTGA TCTGACTTCT 
CGGGATGGAG CCTGGTTCTT GGAGGAACTC TGCAGTATGA GCGGAAACGT CACCTGCTTG 
GTTCAGTTAC TGAAGCAGTG CCACCTGGTG CCACAGGACT TAGATATCCC GAACCCCATG 
GAAGCGTCTG AGACAGTTCA CTTAGCCAAT GGAGTGTATA CCTCACTTCA GGAATTGAAT 
TCGAATTTCC GGCAAATCAT ATTTCCAGAA GCACTTCGAT GTTTAATGAA AGGGGAATAC 
ACGTTAGAAA GTATGCTGCA TGAACTGGAC GGTCTTATTG AGCAGACCAC CGATGGCGTT 
CCCCTGCAGA CTCTAGTGGA ATCTCTTCAG GCCTACTTAA GAAACGCAGC TATGGGACTG 
GAAGAAGAAA CACATGCTCA TTACATCGAT GTTGCCAGAC TACTACATGC TCAGTACGGT 
GAATTAATCC AACCGAGAAA TGGTTCAGTT GATGAAACAC CCAAAATGTC AGCTGGCCAG 
ATGCTTTTGG TAGCATTCGA TGGCATGTTT GCTCAAGTTG AAACTGCTTT CAGCTTATTA 
GTTGAAAAGT TGAACAAGAT GGAAATTCCC ATAGCTTGGC GAAAGATTGA CATCATAAGG 
GAAGCCAGGA GTACTCAAGT TAATTTTTTT GATGATGATA ATCACCGGCA GGTGCTAGAA 
GAGATTTTCT TTCTAAAAAG ACTACAGACT ATTAAGGAGT TCTTCAGGCT CTGTGGTACC 
TTTTCTAAAA CATTGTCAGG ATCAAGTTCA CTTGAAGATC AGAATACTGT GAATGGGCCT 
GTACAGATTG TCAATGTGAA AACCCTTTTT AGAAACTCTT GTTTCAGTGA AGACCAAATG 
GCCAAACCTA TCAAGGCATT CACAGCTGAC TTTGTGAGGC AGCTCTTGAT AGGGCTACCC 
AACCAAGCCC TCGGACTCAC ACTGTGCAGT TTTATCAGTG CTCTGGGTGT AGACATCATT 
GCTCAAGTAG AGGCAAAGGA CTTTGGTGCC GAAAGCAAAG TTTCTGTTGA TGATCTCTGT 
AAGAAAGCGG TGGAACATAA CATCCAGATA GGGAAGTTCT CTCAGCTGGT TATGAACAGG 
GCAACTGTGT TAGCAAGTTC TTACGACACT GCCTGGAAGA AGCATGACTT GGTGCGAAGG 
CTAGAAACCA GTATTTCTTC TTGTAAGACA AGCCTGCAGC GGGTTCAGCT GCATATTGCC 
ATGTTTCAGT GGCAACATGA AGATCTACTT ATCAATAGAC CACAAGCCAT GTCAGTCACA 
CCTCCCCCAC GGTCTGCTAT CCTAACCAGC ATGAAAAAGA AGCTGCATAC CCTGAGCCAG 
ATTGAAACTT CTATTGCAAC AGTTCAGGAG AAGCTAGCTG CACTTGAATC AAGTATTGAA 
CAGCGACTCA AGTGGGCAGG TGGTGCCAAC CCTGCATTGG CCCCTGTACT ACAAGATTTT 
GAAGCAACGA TAGCTGAAAG AAGAAATCTT GTCCTTAAAG AGAGCCAAAG AGCAAGTCAG 
GTCACATTTC TCTGCAGCAA TATCATTCAT TTTGAAAGTT TACGAACAAG AACTGCAGAA 
GCCTTAAACC TGGATGCGGC GTTATTTGAA CTAATCAAGC GATGTCAGCA GATGTGTTCG 
TTTGCATCAC AGTTTAACAG TTCAGTGTCT GAGTTAGAGC TTCGTTTATT ACAGAGAGTG 
GACACTGGTC TTGAACATCC TATTGGCAGC TCTGAATGGC TTTTGTCAGC ACACAAACAG 
TTGACCCAGG ATATGTCTAC TCAGAGGGCA ATTCAGACAG AGAAAGAGCA GCAGATAGAA 
ACGGTCTGTG AAACAATTCA GAATCTGGTT GATAATATAA AGACTGTGCT CACTGGTCAT 
AACCGACAGC TTGGAGATGT CAAACATCTC TTGAAAGCTA TGGCTAAGGA TGAAGAAGCT 
GCTCTGGCAG ATGGTGAAGA TGTTCCCTAT GAGAACAGTG TTAGGCAGTT TTTGGGTGAA 
TATAAATCAT GGCAAGACAA CATTCAAACA GTTCTATTTA CATTAGTCCA GGCTATGGGT 
CAGGTTCGAA GTCAAGAACA CGTTGAAATG CTCCAGGAAA TCACTCCCAC CTTGAAAGAA 
CTGAAAACAC AAAGTCAGAG TATCTATAAT AATTTAGTGA GTTTTGCATC ACCCTTAGTC 
ACCGATGCAA CAAATGAATG TTCGAGTCCA ACGTCATCTG CTACTTATCA GCCATCCTTC 
GCTGCAGCAG TCCGGAGTAA CACTGGCCAG AAGACTCAGC CTGATGTCAT GTCACAGAAT 
GCTAGAAAGC TGATCCAAAA AAATCTTGCT ACATCAGCTG ATACTCCACC AAGCACCGTT 
CCAGGAACTG GCAAGAGTGT TGCTTGTAGT CCTAAAAAGG CAGTCAGAGA CCCTAAAACT 
GGGAAAGCGG TGCAAGAGAG AAACTCCTAT GCAGTGAGTG TGTGGAAGAG AGTGAAAGCC 
AAGTTAGAGG GCCGAGATGT TGATCCGAAT AGGAGGATGT CAGTTGCTGA ACAGGTTGAC 
TATGTCATTA AGGAAGCAAC TAATCTAGAT AACTTGGCTC AGCTGTATGA AGGTTGGACA 
GCCTGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878555.1
CDS	268..11259
Translation	MRFLRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSVQHGSRALATKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGNIKVDFTFMRAKCFLVKGQKYNDLSFQGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_017023066.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023066.2

ORF Insert Sequence:

ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATAATTTAAA ATATTCTTCA 
TCCAGAGATA GAGGTGGTTC TTCCTCTTAT GGACTGCAAC CTTCAAATTC AGCTGTGGTG 
TCTCGGCAAA GGCACGATGA TACCAGAGTC CACGCTGACA TACAGAATGA CGAAAAGGGT 
GGCTACAGTG TCAATGGAGG ATCTGGGGAA AATACTTATG GTCGGAAGTC GTTGGGGCAA 
GAGCTGAGGG TTAACAATGT GACCAGCCCT GAGTTCACCA GTGTTCAGCA TGGCAGTCGT 
GCTTTAGCCA CCAAAGACAT GAGGAAATCA CAGGAGAGAT CGATGTCTTA TTCTGATGAG 
TCTCGACTGT CGAATCTTCT TCGGAGGATC ACCCGGGAAG ACGACAGAGA CCGAAGATTG 
GCTACTGTAA AGCAGTTGAA AGAATTTATT CAGCAACCAG AAAATAAGCT GGTACTAGTT 
AAACAATTGG ATAATATCTT GGCTGCTGTA CATGACGTGC TTAATGAAAG TAGCAAATTG 
CTTCAGGAGT TGAGACAGGA GGGAGCTTGC TGTCTTGGCC TTCTTTGTGC TTCTCTGAGC 
TATGAGGCTG AGAAGATCTT CAAGTGGATT TTTAGCAAAT TTAGCTCATC TGCAAAAGAT 
GAAGTTAAAC TCCTCTACTT ATGTGCCACC TACAAAGCAC TAGAGACTGT AGGAGAAAAG 
AAAGCCTTTT CATCTGTAAT GCAGCTTGTA ATGACCAGCC TGCAGTCTAT TCTTGAAAAT 
GTGGATACAC CAGAATTGCT TTGTAAATGT GTTAAGTGCA TTCTTTTGGT GGCTCGATGT 
TACCCTCATA TTTTCAGCAC TAATTTTAGG GATACAGTTG ATATATTAGT TGGATGGCAT 
ATAGATCATA CTCAGAAACC TTCGCTCACG CAGCAGGTAT CTGGGTGGTT GCAGAGTTTG 
GAGCCATTTT GGGTAGCTGA TCTTGCATTT TCTACTACTC TTCTTGGTCA GTTTCTGGAA 
GACATGGAAG CATATGCTGA GGACCTCAGC CATGTGGCCT CTGGGGAATC AGTGGATGAA 
GATGTCCCTC CTCCATCAGT GTCATTACCA AAGCTGGCTG CACTTCTCCG GGTATTTAGT 
ACTGTGGTGA GGAGCATTGG GGAACGCTTC AGCCCAATTC GGGGTCCTCC AATTACTGAG 
GCATATGTAA CAGATGTTCT GTACAGAGTA ATGAGATGTG TGACGGCTGC AAACCAGGTG 
TTTTTTTCTG AGGCTGTGTT GACAGCTGCT AATGAGTGTG TTGGTGTTTT GCTCGGCAGC 
TTGGATCCTA GCATGACTAT ACATTGTGAC ATGGTCATTA CATATGGATT AGACCAACTG 
GAGAATTGCC AGACTTGTGG TACCGATTAT ATCATCTCAG TCTTGAATTT ACTCACGCTG 
ATTGTTGAAC AGATAAATAC GAAACTGCCA TCATCATTTG TAGAAAAACT GTTTATACCA 
TCATCTAAAC TACTATTCTT GCGTTATCAT AAAGAAAAAG AGGTTGTTGC TGTAGCCCAT 
GCTGTTTATC AAGCAGTGCT CAGCTTGAAG AATATTCCTG TTTTGGAGAC TGCCTATAAG 
TTAATATTGG GAGAAATGAC TTGTGCCCTA AACAACCTCC TACACAGTCT ACAACTTCCT 
GAGGCCTGTT CTGAAATAAA ACATGAGGCT TTTAAGAATC ATGTGTTCAA TGTAGACAAT 
GCAAAATTTG TAGTTATATT TGACCTCAGT GCCCTGACTA CAATTGGAAA TGCCAAAAAC 
TCACTAATAG GGATGTGGGC GCTATCTCCA ACTGTCTTTG CACTTCTGAG TAAGAATCTG 
ATGATTGTGC ACAGTGACCT GGCTGTTCAC TTCCCTGCCA TTCAGTATGC TGTGCTCTAC 
ACATTGTATT CTCATTGTAC CAGGCATGAT CACTTTATCT CTAGTAGCCT CAGTTCTTCC 
TCTCCTTCTT TGTTTGATGG AGCTGTGATT AGCACTGTAA CTACGGCTAC AAAGAAACAT 
TTCTCAATTA TATTAAATCT TCTGGGAATA TTACTTAAGA AAGATAACCT TAACCAGGAC 
ACGAGGAAAC TGTTAATGAC TTGGGCTTTG GAAGCAGCTG TTTTAATGAA GAAGTCTGAA 
ACATACGCAC CTTTATTCTC TCTTCCGTCT TTCCATAAAT TTTGCAAAGG CCTTTTAGCC 
AACACTCTCG TTGAAGATGT GAATATCTGT CTGCAGGCAT GCAGCAGTCT ACATGCTCTG 
TCCTCTTCCT TGCCAGATGA TCTTTTACAG AGATGTGTCG ATGTTTGCCG TGTTCAACTA 
GTGCACAGTG GAACTCGTAT TCGACAAGCA TTTGGAAAAC TGTTGAAATC AATTCCTTTA 
GATGTTGTCC TAAGCAATAA CAATCACACA GAAATTCAAG AAATTTCTTT AGCATTAAGA 
AGTCACATGA GTAAAGCACC AAGTAATACA TTCCACCCCC AAGATTTCTC TGATGTTATT 
AGTTTTATTT TGTATGGGAA CTCTCATAGA ACAGGGAAGG ACAATTGGTT GGAAAGACTG 
TTCTATAGCT GCCAGAGACT GGATAAGCGT GACCAGTCAA CAATTCCACG CAATCTCCTG 
AAGACAGATG CTGTCCTTTG GCAGTGGGCC ATATGGGAAG CTGCACAATT CACTGTTCTT 
TCTAAGCTGA GAACCCCACT GGGCAGAGCT CAAGACACCT TCCAGACAAT TGAAGGTATC 
ATTCGAAGTC TCGCAGCTCA CACATTAAAC CCTGATCAGG ATGTTAGTCA GTGGACAACT 
GCAGACAATG ATGAAGGCCA TGGTAACAAC CAACTTAGAC TTGTTCTTCT TCTGCAGTAT 
CTGGAAAATC TGGAGAAATT AATGTATAAT GCATACGAGG GATGTGCTAA TGCATTAACT 
TCACCTCCCA AGGTCATTAG AACTTTTTTC TATACCAATC GCCAAACTTG TCAGGACTGG 
CTAACGCGGA TTCGACTCTC CATCATGAGG GTAGGATTGT TGGCAGGCCA GCCTGCAGTG 
ACAGTGAGAC ATGGCTTTGA CTTGCTTACA GAGATGAAAA CAACCAGCCT ATCTCAGGGG 
AATGAATTGG AAGTAACCAT TATGATGGTG GTAGAAGCAT TATGTGAACT TCATTGTCCT 
GAAGCTATAC AGGGAATTGC TGTCTGGTCA TCATCTATTG TTGGAAAAAA TCTTCTGTGG 
ATTAACTCAG TGGCTCAACA GGCTGAAGGG AGGTTTGAAA AGGCCTCTGT GGAGTACCAG 
GAACACCTGT GTGCCATGAC AGGTGTTGAT TGCTGCATCT CCAGCTTTGA CAAATCGGTG 
CTCACCTTAG CCAATGCTGG GCGTAACAGT GCCAGCCCGA AACATTCTCT GAATGGTGAA 
TCCAGAAAAA CTGTGCTGTC CAAACCGACT GACTCTTCCC CTGAGGTTAT AAATTATTTA 
GGAAATAAAG CATGTGAGTG CTACATCTCA ATTGCCGATT GGGCTGCTGT GCAGGAATGG 
CAGAACGCTA TCCATGACTT GAAAAAGAGT ACCAGTAGCA CTTCCCTCAA CCTGAAAGCT 
GACTTCAACT ATATAAAATC ATTAAGCAGC TTTGAGTCTG GAAAATTTGT TGAATGTACC 
GAGCAGTTAG AATTGTTACC AGGAGAAAAT ATCAATCTAC TTGCTGGAGG ATCAAAAGAA 
AAAATAGACA TGAAAAAACT GCTTCCTAAC ATGTTAAGTC CGGATCCGAG GGAACTTCAG 
AAATCCATTG AAGTTCAATT GTTAAGAAGT TCTGTTTGTT TGGCAACTGC TTTAAACCCG 
ATAGAACAAG ATCAGAAGTG GCAGTCTATA ACTGAAAATG TGGTAAAGTA CTTGAAGCAA 
ACATCCCGCA TCGCTATTGG ACCTCTGAGA CTTTCTACTT TAACAGTTTC ACAGTCTTTG 
CCAGTTCTAA GTACCTTGCA GCTGTATTGC TCATCTGCTT TGGAGAACAC AGTTTCTAAC 
AGACTTTCAA CAGAGGACTG TCTTATTCCA CTCTTCAGTG AAGCTTTACG TTCATGTAAA 
CAGCATGACG TGAGGCCATG GATGCAGGCA TTAAGGTATA CTATGTACCA GAATCAGTTG 
TTGGAGAAAA TTAAAGAACA AACAGTCCCA ATTAGAAGCC ATCTCATGGA ATTAGGTCTA 
ACAGCAGCAA AATTTGCTAG AAAACGAGGG AATGTGTCCC TTGCAACAAG ACTGCTGGCA 
CAGTGCAGTG AAGTTCAGCT GGGAAAGACC ACCACTGCAC AGGATTTAGT CCAACATTTT 
AAAAAACTAT CAACCCAAGG TCAAGTGGAT GAAAAATGGG GGCCCGAACT TGATATTGAA 
AAAACCAAAT TGCTTTATAC AGCAGGCCAG TCAACACATG CAATGGAAAT GTTGAGTTCT 
TGTGCCATAT CTTTCTGCAA GTCTGTGAAA GCTGAATATG CAGTTGCTAA ATCAATTCTG 
ACACTGGCTA AATGGATCCA GGCAGAATGG AAAGAGATTT CAGGACAGCT GAAACAGGTT 
TACAGAGCTC AGCACCAACA GAACTTCACA GGTCTTTCTA CTTTGTCTAA AAACATACTC 
ACTCTAATAG AACTGCCATC TGTTAATACG ATGGAAGAAG AGTATCCTCG GATCGAGAGT 
GAATCTACAG TGCATATTGG AGTTGGAGAA CCTGACTTCA TTTTGGGACA GTTGTATCAC 
CTGTCTTCAG TACAGGCACC TGAAGTAGCC AAATCTTGGG CAGCGTTGGC CAGCTGGGCT 
TATAGGTGGG GCAGAAAGGT GGTTGACAAT GCCAGTCAGG GAGAAGGTGT TCGTCTGCTG 
CCTAGAGAAA AATCTGAAGT TCAGAATCTA CTTCCAGACA CTATAACTGA GGAAGAGAAA 
GAGAGAATAT ATGGTATTCT TGGACAGGCT GTGTGTCGGC CGGCGGGGAT TCAGGATGAA 
GATATAACAC TTCAGATAAC TGAGAGTGAA GACAACGAAG AAGATGACAT GGTTGATGTT 
ATCTGGCGTC AGTTGATATC AAGCTGCCCA TGGCTTTCAG AACTTGATGA AAGTGCAACT 
GAAGGAGTTA TTAAAGTGTG GAGGAAAGTT GTAGATAGAA TATTCAGCCT GTACAAACTC 
TCTTGCAGTG CATACTTTAC TTTCCTTAAA CTCAACGCTG GTCAAATTCC TTTAGATGAG 
GATGACCCTA GGCTGCATTT AAGTCACAGA GTGGAACAGA GCACTGATGA CATGATTGTG 
ATGGCCACAT TGCGCCTGCT GCGGTTGCTC GTGAAGCATG CTGGTGAGCT TCGGCAGTAT 
CTGGAGCACG GCTTGGAGAC AACACCCACT GCACCATGGA GAGGAATTAT TCCGCAACTT 
TTCTCACGCT TAAACCACCC TGAAGTGTAT GTGCGCCAAA GTATTTGTAA CCTTCTCTGC 
CGTGTGGCTC AAGATTCCCC ACATCTCATA TTGTATCCTG CAATAGTGGG TACCATATCG 
CTTAGTAGTG AATCCCAGGC TTCAGGAAAT AAATTTTCCA CTGCAATTCC AACTTTACTT 
GGCAATATTC AAGGAGAAGA ATTGCTGGTT TCTGAATGTG AGGGAGGAAG TCCTCCTGCA 
TCTCAGGATA GCAATAAGGA TGAACCTAAA AGTGGATTAA ATGAAGACCA AGCCATGATG 
CAGGATTGTT ACAGCAAAAT TGTAGATAAG CTGTCCTCTG CAAACCCCAC CATGGTATTA 
CAGGTTCAGA TGCTCGTGGC TGAACTGCGC AGGGTCACTG TGCTCTGGGA TGAGCTCTGG 
CTGGGAGTTT TGCTGCAACA ACACATGTAT GTCCTGAGAC GAATTCAGCA GCTTGAAGAT 
GAGGTGAAGA GAGTCCAGAA CAACAACACC TTACGCAAAG AAGAGAAAAT TGCAATCATG 
AGGGAGAAGC ACACAGCTTT GATGAAGCCC ATCGTATTTG CTTTGGAGCA TGTGAGGAGT 
ATCACAGCGG CTCCTGCAGA AACACCTCAT GAAAAATGGT TTCAGGATAA CTATGGTGAT 
GCCATTGAAA ATGCCCTAGA AAAACTGAAG ACTCCATTGA ACCCTGCAAA GCCTGGGAGC 
AGCTGGATTC CATTTAAAGA GATAATGCTA AGTTTGCAAC AGAGAGCACA GAAACGTGCA 
AGTTACATCT TGCGTCTTGA AGAAATCAGT CCATGGTTGG CTGCCATGAC TAACACTGAA 
ATTGCTCTTC CTGGGGAAGT CTCAGCCAGA GACACTGTCA CAATCCATAG TGTGGGCGGA 
ACCATCACAA TCTTACCGAC TAAAACCAAG CCAAAGAAAC TTCTCTTTCT TGGATCAGAT 
GGGAAGAGCT ATCCTTATCT TTTCAAAGGA CTGGAGGATT TACATCTGGA TGAGAGAATA 
ATGCAGTTCC TATCTATTGT GAATACCATG TTTGCTACAA TTAATCGCCA AGAAACACCC 
CGGTTCCATG CTCGACACTA TTCTGTAACA CCACTAGGAA CAAGATCAGG ACTAATCCAG 
TGGGTAGATG GAGCCACACC CTTATTTGGT CTTTACAAAC GATGGCAACA ACGGGAAGCT 
GCCTTACAAG CACAAAAGGC CCAAGATTCC TACCAAACTC CTCAGAATCC TGGAATTGTA 
CCCCGTCCTA GTGAACTTTA TTACAGTAAA ATTGGCCCTG CTTTGAAAAC AGTTGGGCTT 
AGCCTGGATG TGTCCCGTCG GGATTGGCCT CTTCATGTAA TGAAGGCAGT ATTGGAAGAG 
TTAATGGAGG CCACACCCCC GAATCTCCTT GCCAAAGAGC TCTGGTCATC TTGCACAACA 
CCTGATGAAT GGTGGAGAGT TACGCAGTCT TATGCAAGAT CTACTGCAGT CATGTCTATG 
GTTGGATACA TAATTGGCCT TGGAGACAGA CATCTGGATA ATGTTCTTAT AGATATGACG 
ACTGGAGAAG TTGTTCACAT AGATTACAAT GTTTGCTTTG AAAAAGGTAA TATAAAAGTA 
GACTTTACAT TTATGAGGGC CAAATGTTTT TTGGTGAAAG GGCAAAAATA TAATGATTTG 
TCTTTTCAAG GTAAAAGCCT TAGAGTTCCT GAGAAAGTAC CTTTTCGAAT GACACAAAAC 
ATTGAAACAG CACTGGGTGT AACTGGAGTA GAAGGTGTAT TTAGGCTTTC ATGTGAGCAG 
GTTTTACACA TTATGCGGCG TGGCAGAGAG ACCCTGCTGA CGCTGCTGGA GGCCTTTGTG 
TACGACCCTC TGGTGGACTG GACAGCAGGA GGCGAGGCTG GGTTTGCTGG TGCTGTCTAT 
GGTGGAGGTG GCCAGCAGGC CGAGAGCAAG CAGAGCAAGA GAGAGATGGA GCGAGAGATC 
ACCCGCAGCC TGTTTTCTTC TAGAGTAGCT GAGATTAAGG TGAACTGGTT TAAGAATAGA 
GATGAGATGC TGGTTGTGCT TCCCAAGTTG GACGGTAGCT TAGATGAATA CCTAAGCTTG 
CAAGAGCAAC TGACAGATGT GGAAAAACTG CAGGGCAAAC TACTGGAGGA AATAGAGTTT 
CTAGAAGGAG CTGAAGGGGT GGATCATCCT TCTCATACTC TGCAACACAG GTATTCTGAG 
CACACCCAAC TACAGACTCA GCAAAGAGCT GTTCAGGAAG CAATCCAGGT GAAGCTGAAT 
GAATTTGAAC AATGGATAAC ACATTATCAG GCTGCATTCA ATAATTTAGA AGCAACACAG 
CTTGCAAGCT TGCTTCAAGA GATAAGCACA CAAATGGACC TTGGTCCTCC AAGTTACGTG 
CCAGCAACAG CCTTTCTGCA GAATGCTGGT CAGGCCCACT TGATTAGCCA GTGCGAGCAG 
CTGGAGGGGG AGGTTGGTGC TCTCCTGCAG CAGAGGCGCT CCGTGCTCCG TGGCTGTCTG 
GAGCAACTGC ATCACTATGC AACCGTGGCC CTGCAGTATC CGAAGGCCAT ATTTCAGAAA 
CATCGAATTG AACAGTGGAA GACCTGGATG GAAGAGCTCA TCTGTAACAC CACAGTAGAG 
CGTTGTCAAG AGCTCTATAG GAAATATGAA ATGCAATATG CTCCCCAGCC ACCCCCAACA 
GTGTGTCAGT TCATCACTGC CACTGAAATG ACCCTGCAGC GATACGCAGC AGACATCAAC 
AGCAGACTTA TTAGACAAGT GGAACGCTTG AAACAGGAAG CTGTCACTGT GCCAGTTTGT 
GAAGATCAGT TGAAAGAAAT TGAACGTTGC ATTAAAGTTT TCCTTCATGA GAATGGAGAA 
GAAGGATCTT TGAGTCTAGC AAGTGTTATT ATTTCTGCCC TTTGTACCCT TACAAGGCGT 
AACCTGATGA TGGAAGGTGC AGCGTCAAGT GCTGGAGAAC AGCTGGTTGA TCTGACTTCT 
CGGGATGGAG CCTGGTTCTT GGAGGAACTC TGCAGTATGA GCGGAAACGT CACCTGCTTG 
GTTCAGTTAC TGAAGCAGTG CCACCTGGTG CCACAGGACT TAGATATCCC GAACCCCATG 
GAAGCGTCTG AGACAGTTCA CTTAGCCAAT GGAGTGTATA CCTCACTTCA GGAATTGAAT 
TCGAATTTCC GGCAAATCAT ATTTCCAGAA GCACTTCGAT GTTTAATGAA AGGGGAATAC 
ACGTTAGAAA GTATGCTGCA TGAACTGGAC GGTCTTATTG AGCAGACCAC CGATGGCGTT 
CCCCTGCAGA CTCTAGTGGA ATCTCTTCAG GCCTACTTAA GAAACGCAGC TATGGGACTG 
GAAGAAGAAA CACATGCTCA TTACATCGAT GTTGCCAGAC TACTACATGC TCAGTACGGT 
GAATTAATCC AACCGAGAAA TGGTTCAGTT GATGAAACAC CCAAAATGTC AGCTGGCCAG 
ATGCTTTTGG TAGCATTCGA TGGCATGTTT GCTCAAGTTG AAACTGCTTT CAGCTTATTA 
GTTGAAAAGT TGAACAAGAT GGAAATTCCC ATAGCTTGGC GAAAGATTGA CATCATAAGG 
GAAGCCAGGA GTACTCAAGT TAATTTTTTT GATGATGATA ATCACCGGCA GGTGCTAGAA 
GAGATTTTCT TTCTAAAAAG ACTACAGACT ATTAAGGAGT TCTTCAGGCT CTGTGGTACC 
TTTTCTAAAA CATTGTCAGG ATCAAGTTCA CTTGAAGATC AGAATACTGT GAATGGGCCT 
GTACAGATTG TCAATGTGAA AACCCTTTTT AGAAACTCTT GTTTCAGTGA AGACCAAATG 
GCCAAACCTA TCAAGGCATT CACAGCTGAC TTTGTGAGGC AGCTCTTGAT AGGGCTACCC 
AACCAAGCCC TCGGACTCAC ACTGTGCAGT TTTATCAGTG CTCTGGGTGT AGACATCATT 
GCTCAAGTAG AGGCAAAGGA CTTTGGTGCC GAAAGCAAAG TTTCTGTTGA TGATCTCTGT 
AAGAAAGCGG TGGAACATAA CATCCAGATA GGGAAGTTCT CTCAGCTGGT TATGAACAGG 
GCAACTGTGT TAGCAAGTTC TTACGACACT GCCTGGAAGA AGCATGACTT GGTGCGAAGG 
CTAGAAACCA GTATTTCTTC TTGTAAGACA AGCCTGCAGC GGGTTCAGCT GCATATTGCC 
ATGTTTCAGT GGCAACATGA AGATCTACTT ATCAATAGAC CACAAGCCAT GTCAGTCACA 
CCTCCCCCAC GGTCTGCTAT CCTAACCAGC ATGAAAAAGA AGCTGCATAC CCTGAGCCAG 
ATTGAAACTT CTATTGCAAC AGTTCAGGAG AAGCTAGCTG CACTTGAATC AAGTATTGAA 
CAGCGACTCA AGTGGGCAGG TGGTGCCAAC CCTGCATTGG CCCCTGTACT ACAAGATTTT 
GAAGCAACGA TAGCTGAAAG AAGAAATCTT GTCCTTAAAG AGAGCCAAAG AGCAAGTCAG 
GTCACATTTC TCTGCAGCAA TATCATTCAT TTTGAAAGTT TACGAACAAG AACTGCAGAA 
GCCTTAAACC TGGATGCGGC GTTATTTGAA CTAATCAAGC GATGTCAGCA GATGTGTTCG 
TTTGCATCAC AGTTTAACAG TTCAGTGTCT GAGTTAGAGC TTCGTTTATT ACAGAGAGTG 
GACACTGGTC TTGAACATCC TATTGGCAGC TCTGAATGGC TTTTGTCAGC ACACAAACAG 
TTGACCCAGG ATATGTCTAC TCAGAGGGCA ATTCAGACAG AGAAAGAGCA GCAGATAGAA 
ACGGTCTGTG AAACAATTCA GAATCTGGTT GATAATATAA AGACTGTGCT CACTGGTCAT 
AACCGACAGC TTGGAGATGT CAAACATCTC TTGAAAGCTA TGGCTAAGGA TGAAGAAGCT 
GCTCTGGCAG ATGGTGAAGA TGTTCCCTAT GAGAACAGTG TTAGGCAGTT TTTGGGTGAA 
TATAAATCAT GGCAAGACAA CATTCAAACA GTTCTATTTA CATTAGTCCA GGCTATGGGT 
CAGGTTCGAA GTCAAGAACA CGTTGAAATG CTCCAGGAAA TCACTCCCAC CTTGAAAGAA 
CTGAAAACAC AAAGTCAGAG TATCTATAAT AATTTAGTGA GTTTTGCATC ACCCTTAGTC 
ACCGATGCAA CAAATGAATG TTCGAGTCCA ACGTCATCTG CTACTTATCA GCCATCCTTC 
GCTGCAGCAG TCCGGAGTAA CACTGGCCAG AAGACTCAGC CTGATGTCAT GTCACAGAAT 
GCTAGAAAGC TGATCCAAAA AAATCTTGCT ACATCAGCTG ATACTCCACC AAGCACCGTT 
CCAGGAACTG GCAAGAGTGT TGCTTGTAGT CCTAAAAAGG CAGTCAGAGA CCCTAAAACT 
GGGAAAGCGG TGCAAGAGAG AAACTCCTAT GCAGTGAGTG TGTGGAAGAG AGTGAAAGCC 
AAGTTAGAGG GCCGAGATGT TGATCCGAAT AGGAGGATGT CAGTTGCTGA ACAGGTTGAC 
TATGTCATTA AGGAAGCAAC TAATCTAGAT AACTTGGCTC AGCTGTATGA AGGTTGGACA 
GCCTGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_005255184.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255241.1 Homo sapiens SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X5, mRNA.

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CloneID	OHu49090
Clone ID Related Accession (Same CDS sequence)	XM_005255184.3 , XM_005255184.4
Accession Version	XM_005255184.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005255184.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGAGAG ATCGATGTCT TATTCTGATG AGTCTCGACT GTCGAATCTT 
CTTCGGAGGA TCACCCGGGA AGACGACAGA GACCGAAGAT TGGCTACTGT AAAGCAGTTG 
AAAGAATTTA TTCAGCAACC AGAAAATAAG CTGGTACTAG TTAAACAATT GGATAATATC 
TTGGCTGCTG TACATGACGT GCTTAATGAA AGTAGCAAAT TGCTTCAGGA GTTGAGACAG 
GAGGGAGCTT GCTGTCTTGG CCTTCTTTGT GCTTCTCTGA GCTATGAGGC TGAGAAGATC 
TTCAAGTGGA TTTTTAGCAA ATTTAGCTCA TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC 
TTATGTGCCA CCTACAAAGC ACTAGAGACT GTAGGAGAAA AGAAAGCCTT TTCATCTGTA 
ATGCAGCTTG TAATGACCAG CCTGCAGTCT ATTCTTGAAA ATGTGGATAC ACCAGAATTG 
CTTTGTAAAT GTGTTAAGTG CATTCTTTTG GTGGCTCGAT GTTACCCTCA TATTTTCAGC 
ACTAATTTTA GGGATACAGT TGATATATTA GTTGGATGGC ATATAGATCA TACTCAGAAA 
CCTTCGCTCA CGCAGCAGGT ATCTGGGTGG TTGCAGAGTT TGGAGCCATT TTGGGTAGCT 
GATCTTGCAT TTTCTACTAC TCTTCTTGGT CAGTTTCTGG AAGACATGGA AGCATATGCT 
GAGGACCTCA GCCATGTGGC CTCTGGGGAA TCAGTGGATG AAGATGTCCC TCCTCCATCA 
GTGTCATTAC CAAAGCTGGC TGCACTTCTC CGGGTATTTA GTACTGTGGT GAGGAGCATT 
GGGGAACGCT TCAGCCCAAT TCGGGGTCCT CCAATTACTG AGGCATATGT AACAGATGTT 
CTGTACAGAG TAATGAGATG TGTGACGGCT GCAAACCAGG TGTTTTTTTC TGAGGCTGTG 
TTGACAGCTG CTAATGAGTG TGTTGGTGTT TTGCTCGGCA GCTTGGATCC TAGCATGACT 
ATACATTGTG ACATGGTCAT TACATATGGA TTAGACCAAC TGGAGAATTG CCAGACTTGT 
GGTACCGATT ATATCATCTC AGTCTTGAAT TTACTCACGC TGATTGTTGA ACAGATAAAT 
ACGAAACTGC CATCATCATT TGTAGAAAAA CTGTTTATAC CATCATCTAA ACTACTATTC 
TTGCGTTATC ATAAAGAAAA AGAGGTTGTT GCTGTAGCCC ATGCTGTTTA TCAAGCAGTG 
CTCAGCTTGA AGAATATTCC TGTTTTGGAG ACTGCCTATA AGTTAATATT GGGAGAAATG 
ACTTGTGCCC TAAACAACCT CCTACACAGT CTACAACTTC CTGAGGCCTG TTCTGAAATA 
AAACATGAGG CTTTTAAGAA TCATGTGTTC AATGTAGACA ATGCAAAATT TGTAGTTATA 
TTTGACCTCA GTGCCCTGAC TACAATTGGA AATGCCAAAA ACTCACTAAT AGGGATGTGG 
GCGCTATCTC CAACTGTCTT TGCACTTCTG AGTAAGAATC TGATGATTGT GCACAGTGAC 
CTGGCTGTTC ACTTCCCTGC CATTCAGTAT GCTGTGCTCT ACACATTGTA TTCTCATTGT 
ACCAGGCATG ATCACTTTAT CTCTAGTAGC CTCAGTTCTT CCTCTCCTTC TTTGTTTGAT 
GGAGCTGTGA TTAGCACTGT AACTACGGCT ACAAAGAAAC ATTTCTCAAT TATATTAAAT 
CTTCTGGGAA TATTACTTAA GAAAGATAAC CTTAACCAGG ACACGAGGAA ACTGTTAATG 
ACTTGGGCTT TGGAAGCAGC TGTTTTAATG AAGAAGTCTG AAACATACGC ACCTTTATTC 
TCTCTTCCGT CTTTCCATAA ATTTTGCAAA GGCCTTTTAG CCAACACTCT CGTTGAAGAT 
GTGAATATCT GTCTGCAGGC ATGCAGCAGT CTACATGCTC TGTCCTCTTC CTTGCCAGAT 
GATCTTTTAC AGAGATGTGT CGATGTTTGC CGTGTTCAAC TAGTGCACAG TGGAACTCGT 
ATTCGACAAG CATTTGGAAA ACTGTTGAAA TCAATTCCTT TAGATGTTGT CCTAAGCAAT 
AACAATCACA CAGAAATTCA AGAAATTTCT TTAGCATTAA GAAGTCACAT GAGTAAAGCA 
CCAAGTAATA CATTCCACCC CCAAGATTTC TCTGATGTTA TTAGTTTTAT TTTGTATGGG 
AACTCTCATA GAACAGGGAA GGACAATTGG TTGGAAAGAC TGTTCTATAG CTGCCAGAGA 
CTGGATAAGC GTGACCAGTC AACAATTCCA CGCAATCTCC TGAAGACAGA TGCTGTCCTT 
TGGCAGTGGG CCATATGGGA AGCTGCACAA TTCACTGTTC TTTCTAAGCT GAGAACCCCA 
CTGGGCAGAG CTCAAGACAC CTTCCAGACA ATTGAAGGTA TCATTCGAAG TCTCGCAGCT 
CACACATTAA ACCCTGATCA GGATGTTAGT CAGTGGACAA CTGCAGACAA TGATGAAGGC 
CATGGTAACA ACCAACTTAG ACTTGTTCTT CTTCTGCAGT ATCTGGAAAA TCTGGAGAAA 
TTAATGTATA ATGCATACGA GGGATGTGCT AATGCATTAA CTTCACCTCC CAAGGTCATT 
AGAACTTTTT TCTATACCAA TCGCCAAACT TGTCAGGACT GGCTAACGCG GATTCGACTC 
TCCATCATGA GGGTAGGATT GTTGGCAGGC CAGCCTGCAG TGACAGTGAG ACATGGCTTT 
GACTTGCTTA CAGAGATGAA AACAACCAGC CTATCTCAGG GGAATGAATT GGAAGTAACC 
ATTATGATGG TGGTAGAAGC ATTATGTGAA CTTCATTGTC CTGAAGCTAT ACAGGGAATT 
GCTGTCTGGT CATCATCTAT TGTTGGAAAA AATCTTCTGT GGATTAACTC AGTGGCTCAA 
CAGGCTGAAG GGAGGTTTGA AAAGGCCTCT GTGGAGTACC AGGAACACCT GTGTGCCATG 
ACAGGTGTTG ATTGCTGCAT CTCCAGCTTT GACAAATCGG TGCTCACCTT AGCCAATGCT 
GGGCGTAACA GTGCCAGCCC GAAACATTCT CTGAATGGTG AATCCAGAAA AACTGTGCTG 
TCCAAACCGA CTGACTCTTC CCCTGAGGTT ATAAATTATT TAGGAAATAA AGCATGTGAG 
TGCTACATCT CAATTGCCGA TTGGGCTGCT GTGCAGGAAT GGCAGAACGC TATCCATGAC 
TTGAAAAAGA GTACCAGTAG CACTTCCCTC AACCTGAAAG CTGACTTCAA CTATATAAAA 
TCATTAAGCA GCTTTGAGTC TGGAAAATTT GTTGAATGTA CCGAGCAGTT AGAATTGTTA 
CCAGGAGAAA ATATCAATCT ACTTGCTGGA GGATCAAAAG AAAAAATAGA CATGAAAAAA 
CTGCTTCCTA ACATGTTAAG TCCGGATCCG AGGGAACTTC AGAAATCCAT TGAAGTTCAA 
TTGTTAAGAA GTTCTGTTTG TTTGGCAACT GCTTTAAACC CGATAGAACA AGATCAGAAG 
TGGCAGTCTA TAACTGAAAA TGTGGTAAAG TACTTGAAGC AAACATCCCG CATCGCTATT 
GGACCTCTGA GACTTTCTAC TTTAACAGTT TCACAGTCTT TGCCAGTTCT AAGTACCTTG 
CAGCTGTATT GCTCATCTGC TTTGGAGAAC ACAGTTTCTA ACAGACTTTC AACAGAGGAC 
TGTCTTATTC CACTCTTCAG TGAAGCTTTA CGTTCATGTA AACAGCATGA CGTGAGGCCA 
TGGATGCAGG CATTAAGGTA TACTATGTAC CAGAATCAGT TGTTGGAGAA AATTAAAGAA 
CAAACAGTCC CAATTAGAAG CCATCTCATG GAATTAGGTC TAACAGCAGC AAAATTTGCT 
AGAAAACGAG GGAATGTGTC CCTTGCAACA AGACTGCTGG CACAGTGCAG TGAAGTTCAG 
CTGGGAAAGA CCACCACTGC ACAGGATTTA GTCCAACATT TTAAAAAACT ATCAACCCAA 
GGTCAAGTGG ATGAAAAATG GGGGCCCGAA CTTGATATTG AAAAAACCAA ATTGCTTTAT 
ACAGCAGGCC AGTCAACACA TGCAATGGAA ATGTTGAGTT CTTGTGCCAT ATCTTTCTGC 
AAGTCTGTGA AAGCTGAATA TGCAGTTGCT AAATCAATTC TGACACTGGC TAAATGGATC 
CAGGCAGAAT GGAAAGAGAT TTCAGGACAG CTGAAACAGG TTTACAGAGC TCAGCACCAA 
CAGAACTTCA CAGGTCTTTC TACTTTGTCT AAAAACATAC TCACTCTAAT AGAACTGCCA 
TCTGTTAATA CGATGGAAGA AGAGTATCCT CGGATCGAGA GTGAATCTAC AGTGCATATT 
GGAGTTGGAG AACCTGACTT CATTTTGGGA CAGTTGTATC ACCTGTCTTC AGTACAGGCA 
CCTGAAGTAG CCAAATCTTG GGCAGCGTTG GCCAGCTGGG CTTATAGGTG GGGCAGAAAG 
GTGGTTGACA ATGCCAGTCA GGGAGAAGGT GTTCGTCTGC TGCCTAGAGA AAAATCTGAA 
GTTCAGAATC TACTTCCAGA CACTATAACT GAGGAAGAGA AAGAGAGAAT ATATGGTATT 
CTTGGACAGG CTGTGTGTCG GCCGGCGGGG ATTCAGGATG AAGATATAAC ACTTCAGATA 
ACTGAGAGTG AAGACAACGA AGAAGATGAC ATGGTTGATG TTATCTGGCG TCAGTTGATA 
TCAAGCTGCC CATGGCTTTC AGAACTTGAT GAAAGTGCAA CTGAAGGAGT TATTAAAGTG 
TGGAGGAAAG TTGTAGATAG AATATTCAGC CTGTACAAAC TCTCTTGCAG TGCATACTTT 
ACTTTCCTTA AACTCAACGC TGGTCAAATT CCTTTAGATG AGGATGACCC TAGGCTGCAT 
TTAAGTCACA GAGTGGAACA GAGCACTGAT GACATGATTG TGATGGCCAC ATTGCGCCTG 
CTGCGGTTGC TCGTGAAGCA TGCTGGTGAG CTTCGGCAGT ATCTGGAGCA CGGCTTGGAG 
ACAACACCCA CTGCACCATG GAGAGGAATT ATTCCGCAAC TTTTCTCACG CTTAAACCAC 
CCTGAAGTGT ATGTGCGCCA AAGTATTTGT AACCTTCTCT GCCGTGTGGC TCAAGATTCC 
CCACATCTCA TATTGTATCC TGCAATAGTG GGTACCATAT CGCTTAGTAG TGAATCCCAG 
GCTTCAGGAA ATAAATTTTC CACTGCAATT CCAACTTTAC TTGGCAATAT TCAAGGAGAA 
GAATTGCTGG TTTCTGAATG TGAGGGAGGA AGTCCTCCTG CATCTCAGGA TAGCAATAAG 
GATGAACCTA AAAGTGGATT AAATGAAGAC CAAGCCATGA TGCAGGATTG TTACAGCAAA 
ATTGTAGATA AGCTGTCCTC TGCAAACCCC ACCATGGTAT TACAGGTTCA GATGCTCGTG 
GCTGAACTGC GCAGGGTCAC TGTGCTCTGG GATGAGCTCT GGCTGGGAGT TTTGCTGCAA 
CAACACATGT ATGTCCTGAG ACGAATTCAG CAGCTTGAAG ATGAGGTGAA GAGAGTCCAG 
AACAACAACA CCTTACGCAA AGAAGAGAAA ATTGCAATCA TGAGGGAGAA GCACACAGCT 
TTGATGAAGC CCATCGTATT TGCTTTGGAG CATGTGAGGA GTATCACAGC GGCTCCTGCA 
GAAACACCTC ATGAAAAATG GTTTCAGGAT AACTATGGTG ATGCCATTGA AAATGCCCTA 
GAAAAACTGA AGACTCCATT GAACCCTGCA AAGCCTGGGA GCAGCTGGAT TCCATTTAAA 
GAGATAATGC TAAGTTTGCA ACAGAGAGCA CAGAAACGTG CAAGTTACAT CTTGCGTCTT 
GAAGAAATCA GTCCATGGTT GGCTGCCATG ACTAACACTG AAATTGCTCT TCCTGGGGAA 
GTCTCAGCCA GAGACACTGT CACAATCCAT AGTGTGGGCG GAACCATCAC AATCTTACCG 
ACTAAAACCA AGCCAAAGAA ACTTCTCTTT CTTGGATCAG ATGGGAAGAG CTATCCTTAT 
CTTTTCAAAG GACTGGAGGA TTTACATCTG GATGAGAGAA TAATGCAGTT CCTATCTATT 
GTGAATACCA TGTTTGCTAC AATTAATCGC CAAGAAACAC CCCGGTTCCA TGCTCGACAC 
TATTCTGTAA CACCACTAGG AACAAGATCA GGACTAATCC AGTGGGTAGA TGGAGCCACA 
CCCTTATTTG GTCTTTACAA ACGATGGCAA CAACGGGAAG CTGCCTTACA AGCACAAAAG 
GCCCAAGATT CCTACCAAAC TCCTCAGAAT CCTGGAATTG TACCCCGTCC TAGTGAACTT 
TATTACAGTA AAATTGGCCC TGCTTTGAAA ACAGTTGGGC TTAGCCTGGA TGTGTCCCGT 
CGGGATTGGC CTCTTCATGT AATGAAGGCA GTATTGGAAG AGTTAATGGA GGCCACACCC 
CCGAATCTCC TTGCCAAAGA GCTCTGGTCA TCTTGCACAA CACCTGATGA ATGGTGGAGA 
GTTACGCAGT CTTATGCAAG ATCTACTGCA GTCATGTCTA TGGTTGGATA CATAATTGGC 
CTTGGAGACA GACATCTGGA TAATGTTCTT ATAGATATGA CGACTGGAGA AGTTGTTCAC 
ATAGATTACA ATGTTTGCTT TGAAAAAGGT AATATAAAAG TAGACTTTAC ATTTATGAGG 
GCCAAATGTT TTTTGGTGAA AGGGCAAAAA TATAATGATT TGTCTTTTCA AGGTAAAAGC 
CTTAGAGTTC CTGAGAAAGT ACCTTTTCGA ATGACACAAA ACATTGAAAC AGCACTGGGT 
GTAACTGGAG TAGAAGGTGT ATTTAGGCTT TCATGTGAGC AGGTTTTACA CATTATGCGG 
CGTGGCAGAG AGACCCTGCT GACGCTGCTG GAGGCCTTTG TGTACGACCC TCTGGTGGAC 
TGGACAGCAG GAGGCGAGGC TGGGTTTGCT GGTGCTGTCT ATGGTGGAGG TGGCCAGCAG 
GCCGAGAGCA AGCAGAGCAA GAGAGAGATG GAGCGAGAGA TCACCCGCAG CCTGTTTTCT 
TCTAGAGTAG CTGAGATTAA GGTGAACTGG TTTAAGAATA GAGATGAGAT GCTGGTTGTG 
CTTCCCAAGT TGGACGGTAG CTTAGATGAA TACCTAAGCT TGCAAGAGCA ACTGACAGAT 
GTGGAAAAAC TGCAGGGCAA ACTACTGGAG GAAATAGAGT TTCTAGAAGG AGCTGAAGGG 
GTGGATCATC CTTCTCATAC TCTGCAACAC AGGTATTCTG AGCACACCCA ACTACAGACT 
CAGCAAAGAG CTGTTCAGGA AGCAATCCAG GTGAAGCTGA ATGAATTTGA ACAATGGATA 
ACACATTATC AGGCTGCATT CAATAATTTA GAAGCAACAC AGCTTGCAAG CTTGCTTCAA 
GAGATAAGCA CACAAATGGA CCTTGGTCCT CCAAGTTACG TGCCAGCAAC AGCCTTTCTG 
CAGAATGCTG GTCAGGCCCA CTTGATTAGC CAGTGCGAGC AGCTGGAGGG GGAGGTTGGT 
GCTCTCCTGC AGCAGAGGCG CTCCGTGCTC CGTGGCTGTC TGGAGCAACT GCATCACTAT 
GCAACCGTGG CCCTGCAGTA TCCGAAGGCC ATATTTCAGA AACATCGAAT TGAACAGTGG 
AAGACCTGGA TGGAAGAGCT CATCTGTAAC ACCACAGTAG AGCGTTGTCA AGAGCTCTAT 
AGGAAATATG AAATGCAATA TGCTCCCCAG CCACCCCCAA CAGTGTGTCA GTTCATCACT 
GCCACTGAAA TGACCCTGCA GCGATACGCA GCAGACATCA ACAGCAGACT TATTAGACAA 
GTGGAACGCT TGAAACAGGA AGCTGTCACT GTGCCAGTTT GTGAAGATCA GTTGAAAGAA 
ATTGAACGTT GCATTAAAGT TTTCCTTCAT GAGAATGGAG AAGAAGGATC TTTGAGTCTA 
GCAAGTGTTA TTATTTCTGC CCTTTGTACC CTTACAAGGC GTAACCTGAT GATGGAAGGT 
GCAGCGTCAA GTGCTGGAGA ACAGCTGGTT GATCTGACTT CTCGGGATGG AGCCTGGTTC 
TTGGAGGAAC TCTGCAGTAT GAGCGGAAAC GTCACCTGCT TGGTTCAGTT ACTGAAGCAG 
TGCCACCTGG TGCCACAGGA CTTAGATATC CCGAACCCCA TGGAAGCGTC TGAGACAGTT 
CACTTAGCCA ATGGAGTGTA TACCTCACTT CAGGAATTGA ATTCGAATTT CCGGCAAATC 
ATATTTCCAG AAGCACTTCG ATGTTTAATG AAAGGGGAAT ACACGTTAGA AAGTATGCTG 
CATGAACTGG ACGGTCTTAT TGAGCAGACC ACCGATGGCG TTCCCCTGCA GACTCTAGTG 
GAATCTCTTC AGGCCTACTT AAGAAACGCA GCTATGGGAC TGGAAGAAGA AACACATGCT 
CATTACATCG ATGTTGCCAG ACTACTACAT GCTCAGTACG GTGAATTAAT CCAACCGAGA 
AATGGTTCAG TTGATGAAAC ACCCAAAATG TCAGCTGGCC AGATGCTTTT GGTAGCATTC 
GATGGCATGT TTGCTCAAGT TGAAACTGCT TTCAGCTTAT TAGTTGAAAA GTTGAACAAG 
ATGGAAATTC CCATAGCTTG GCGAAAGATT GACATCATAA GGGAAGCCAG GAGTACTCAA 
GTTAATTTTT TTGATGATGA TAATCACCGG CAGGTGCTAG AAGAGATTTT CTTTCTAAAA 
AGACTACAGA CTATTAAGGA GTTCTTCAGG CTCTGTGGTA CCTTTTCTAA AACATTGTCA 
GGATCAAGTT CACTTGAAGA TCAGAATACT GTGAATGGGC CTGTACAGAT TGTCAATGTG 
AAAACCCTTT TTAGAAACTC TTGTTTCAGT GAAGACCAAA TGGCCAAACC TATCAAGGCA 
TTCACAGCTG ACTTTGTGAG GCAGCTCTTG ATAGGGCTAC CCAACCAAGC CCTCGGACTC 
ACACTGTGCA GTTTTATCAG TGCTCTGGGT GTAGACATCA TTGCTCAAGT AGAGGCAAAG 
GACTTTGGTG CCGAAAGCAA AGTTTCTGTT GATGATCTCT GTAAGAAAGC GGTGGAACAT 
AACATCCAGA TAGGGAAGTT CTCTCAGCTG GTTATGAACA GGGCAACTGT GTTAGCAAGT 
TCTTACGACA CTGCCTGGAA GAAGCATGAC TTGGTGCGAA GGCTAGAAAC CAGTATTTCT 
TCTTGTAAGA CAAGCCTGCA GCGGGTTCAG CTGCATATTG CCATGTTTCA GTGGCAACAT 
GAAGATCTAC TTATCAATAG ACCACAAGCC ATGTCAGTCA CACCTCCCCC ACGGTCTGCT 
ATCCTAACCA GCATGAAAAA GAAGCTGCAT ACCCTGAGCC AGATTGAAAC TTCTATTGCA 
ACAGTTCAGG AGAAGCTAGC TGCACTTGAA TCAAGTATTG AACAGCGACT CAAGTGGGCA 
GGTGGTGCCA ACCCTGCATT GGCCCCTGTA CTACAAGATT TTGAAGCAAC GATAGCTGAA 
AGAAGAAATC TTGTCCTTAA AGAGAGCCAA AGAGCAAGTC AGGTCACATT TCTCTGCAGC 
AATATCATTC ATTTTGAAAG TTTACGAACA AGAACTGCAG AAGCCTTAAA CCTGGATGCG 
GCGTTATTTG AACTAATCAA GCGATGTCAG CAGATGTGTT CGTTTGCATC ACAGTTTAAC 
AGTTCAGTGT CTGAGTTAGA GCTTCGTTTA TTACAGAGAG TGGACACTGG TCTTGAACAT 
CCTATTGGCA GCTCTGAATG GCTTTTGTCA GCACACAAAC AGTTGACCCA GGATATGTCT 
ACTCAGAGGG CAATTCAGAC AGAGAAAGAG CAGCAGATAG AAACGGTCTG TGAAACAATT 
CAGAATCTGG TTGATAATAT AAAGACTGTG CTCACTGGTC ATAACCGACA GCTTGGAGAT 
GTCAAACATC TCTTGAAAGC TATGGCTAAG GATGAAGAAG CTGCTCTGGC AGATGGTGAA 
GATGTTCCCT ATGAGAACAG TGTTAGGCAG TTTTTGGGTG AATATAAATC ATGGCAAGAC 
AACATTCAAA CAGTTCTATT TACATTAGTC CAGGCTATGG GTCAGGTTCG AAGTCAAGAA 
CACGTTGAAA TGCTCCAGGA AATCACTCCC ACCTTGAAAG AACTGAAAAC ACAAAGTCAG 
AGTATCTATA ATAATTTAGT GAGTTTTGCA TCACCCTTAG TCACCGATGC AACAAATGAA 
TGTTCGAGTC CAACGTCATC TGCTACTTAT CAGCCATCCT TCGCTGCAGC AGTCCGGAGT 
AACACTGGCC AGAAGACTCA GCCTGATGTC ATGTCACAGA ATGCTAGAAA GCTGATCCAA 
AAAAATCTTG CTACATCAGC TGATACTCCA CCAAGCACCG TTCCAGGAAC TGGCAAGAGT 
GTTGCTTGTA GTCCTAAAAA GGCAGTCAGA GACCCTAAAA CTGGGAAAGC GGTGCAAGAG 
AGAAACTCCT ATGCAGTGAG TGTGTGGAAG AGAGTGAAAG CCAAGTTAGA GGGCCGAGAT 
GTTGATCCGA ATAGGAGGAT GTCAGTTGCT GAACAGGTTG ACTATGTCAT TAAGGAAGCA 
ACTAATCTAG ATAACTTGGC TCAGCTGTAT GAAGGTTGGA CAGCCTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255241.1
CDS	377..11290
Translation	MSRRAPGSRLSSGGGGGGTKYPRSWNDWQPRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGNIKVDFTFMRAKCFLVKGQKYNDLSFQGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_005255184.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255184.3

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGAGAG ATCGATGTCT TATTCTGATG AGTCTCGACT GTCGAATCTT 
CTTCGGAGGA TCACCCGGGA AGACGACAGA GACCGAAGAT TGGCTACTGT AAAGCAGTTG 
AAAGAATTTA TTCAGCAACC AGAAAATAAG CTGGTACTAG TTAAACAATT GGATAATATC 
TTGGCTGCTG TACATGACGT GCTTAATGAA AGTAGCAAAT TGCTTCAGGA GTTGAGACAG 
GAGGGAGCTT GCTGTCTTGG CCTTCTTTGT GCTTCTCTGA GCTATGAGGC TGAGAAGATC 
TTCAAGTGGA TTTTTAGCAA ATTTAGCTCA TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC 
TTATGTGCCA CCTACAAAGC ACTAGAGACT GTAGGAGAAA AGAAAGCCTT TTCATCTGTA 
ATGCAGCTTG TAATGACCAG CCTGCAGTCT ATTCTTGAAA ATGTGGATAC ACCAGAATTG 
CTTTGTAAAT GTGTTAAGTG CATTCTTTTG GTGGCTCGAT GTTACCCTCA TATTTTCAGC 
ACTAATTTTA GGGATACAGT TGATATATTA GTTGGATGGC ATATAGATCA TACTCAGAAA 
CCTTCGCTCA CGCAGCAGGT ATCTGGGTGG TTGCAGAGTT TGGAGCCATT TTGGGTAGCT 
GATCTTGCAT TTTCTACTAC TCTTCTTGGT CAGTTTCTGG AAGACATGGA AGCATATGCT 
GAGGACCTCA GCCATGTGGC CTCTGGGGAA TCAGTGGATG AAGATGTCCC TCCTCCATCA 
GTGTCATTAC CAAAGCTGGC TGCACTTCTC CGGGTATTTA GTACTGTGGT GAGGAGCATT 
GGGGAACGCT TCAGCCCAAT TCGGGGTCCT CCAATTACTG AGGCATATGT AACAGATGTT 
CTGTACAGAG TAATGAGATG TGTGACGGCT GCAAACCAGG TGTTTTTTTC TGAGGCTGTG 
TTGACAGCTG CTAATGAGTG TGTTGGTGTT TTGCTCGGCA GCTTGGATCC TAGCATGACT 
ATACATTGTG ACATGGTCAT TACATATGGA TTAGACCAAC TGGAGAATTG CCAGACTTGT 
GGTACCGATT ATATCATCTC AGTCTTGAAT TTACTCACGC TGATTGTTGA ACAGATAAAT 
ACGAAACTGC CATCATCATT TGTAGAAAAA CTGTTTATAC CATCATCTAA ACTACTATTC 
TTGCGTTATC ATAAAGAAAA AGAGGTTGTT GCTGTAGCCC ATGCTGTTTA TCAAGCAGTG 
CTCAGCTTGA AGAATATTCC TGTTTTGGAG ACTGCCTATA AGTTAATATT GGGAGAAATG 
ACTTGTGCCC TAAACAACCT CCTACACAGT CTACAACTTC CTGAGGCCTG TTCTGAAATA 
AAACATGAGG CTTTTAAGAA TCATGTGTTC AATGTAGACA ATGCAAAATT TGTAGTTATA 
TTTGACCTCA GTGCCCTGAC TACAATTGGA AATGCCAAAA ACTCACTAAT AGGGATGTGG 
GCGCTATCTC CAACTGTCTT TGCACTTCTG AGTAAGAATC TGATGATTGT GCACAGTGAC 
CTGGCTGTTC ACTTCCCTGC CATTCAGTAT GCTGTGCTCT ACACATTGTA TTCTCATTGT 
ACCAGGCATG ATCACTTTAT CTCTAGTAGC CTCAGTTCTT CCTCTCCTTC TTTGTTTGAT 
GGAGCTGTGA TTAGCACTGT AACTACGGCT ACAAAGAAAC ATTTCTCAAT TATATTAAAT 
CTTCTGGGAA TATTACTTAA GAAAGATAAC CTTAACCAGG ACACGAGGAA ACTGTTAATG 
ACTTGGGCTT TGGAAGCAGC TGTTTTAATG AAGAAGTCTG AAACATACGC ACCTTTATTC 
TCTCTTCCGT CTTTCCATAA ATTTTGCAAA GGCCTTTTAG CCAACACTCT CGTTGAAGAT 
GTGAATATCT GTCTGCAGGC ATGCAGCAGT CTACATGCTC TGTCCTCTTC CTTGCCAGAT 
GATCTTTTAC AGAGATGTGT CGATGTTTGC CGTGTTCAAC TAGTGCACAG TGGAACTCGT 
ATTCGACAAG CATTTGGAAA ACTGTTGAAA TCAATTCCTT TAGATGTTGT CCTAAGCAAT 
AACAATCACA CAGAAATTCA AGAAATTTCT TTAGCATTAA GAAGTCACAT GAGTAAAGCA 
CCAAGTAATA CATTCCACCC CCAAGATTTC TCTGATGTTA TTAGTTTTAT TTTGTATGGG 
AACTCTCATA GAACAGGGAA GGACAATTGG TTGGAAAGAC TGTTCTATAG CTGCCAGAGA 
CTGGATAAGC GTGACCAGTC AACAATTCCA CGCAATCTCC TGAAGACAGA TGCTGTCCTT 
TGGCAGTGGG CCATATGGGA AGCTGCACAA TTCACTGTTC TTTCTAAGCT GAGAACCCCA 
CTGGGCAGAG CTCAAGACAC CTTCCAGACA ATTGAAGGTA TCATTCGAAG TCTCGCAGCT 
CACACATTAA ACCCTGATCA GGATGTTAGT CAGTGGACAA CTGCAGACAA TGATGAAGGC 
CATGGTAACA ACCAACTTAG ACTTGTTCTT CTTCTGCAGT ATCTGGAAAA TCTGGAGAAA 
TTAATGTATA ATGCATACGA GGGATGTGCT AATGCATTAA CTTCACCTCC CAAGGTCATT 
AGAACTTTTT TCTATACCAA TCGCCAAACT TGTCAGGACT GGCTAACGCG GATTCGACTC 
TCCATCATGA GGGTAGGATT GTTGGCAGGC CAGCCTGCAG TGACAGTGAG ACATGGCTTT 
GACTTGCTTA CAGAGATGAA AACAACCAGC CTATCTCAGG GGAATGAATT GGAAGTAACC 
ATTATGATGG TGGTAGAAGC ATTATGTGAA CTTCATTGTC CTGAAGCTAT ACAGGGAATT 
GCTGTCTGGT CATCATCTAT TGTTGGAAAA AATCTTCTGT GGATTAACTC AGTGGCTCAA 
CAGGCTGAAG GGAGGTTTGA AAAGGCCTCT GTGGAGTACC AGGAACACCT GTGTGCCATG 
ACAGGTGTTG ATTGCTGCAT CTCCAGCTTT GACAAATCGG TGCTCACCTT AGCCAATGCT 
GGGCGTAACA GTGCCAGCCC GAAACATTCT CTGAATGGTG AATCCAGAAA AACTGTGCTG 
TCCAAACCGA CTGACTCTTC CCCTGAGGTT ATAAATTATT TAGGAAATAA AGCATGTGAG 
TGCTACATCT CAATTGCCGA TTGGGCTGCT GTGCAGGAAT GGCAGAACGC TATCCATGAC 
TTGAAAAAGA GTACCAGTAG CACTTCCCTC AACCTGAAAG CTGACTTCAA CTATATAAAA 
TCATTAAGCA GCTTTGAGTC TGGAAAATTT GTTGAATGTA CCGAGCAGTT AGAATTGTTA 
CCAGGAGAAA ATATCAATCT ACTTGCTGGA GGATCAAAAG AAAAAATAGA CATGAAAAAA 
CTGCTTCCTA ACATGTTAAG TCCGGATCCG AGGGAACTTC AGAAATCCAT TGAAGTTCAA 
TTGTTAAGAA GTTCTGTTTG TTTGGCAACT GCTTTAAACC CGATAGAACA AGATCAGAAG 
TGGCAGTCTA TAACTGAAAA TGTGGTAAAG TACTTGAAGC AAACATCCCG CATCGCTATT 
GGACCTCTGA GACTTTCTAC TTTAACAGTT TCACAGTCTT TGCCAGTTCT AAGTACCTTG 
CAGCTGTATT GCTCATCTGC TTTGGAGAAC ACAGTTTCTA ACAGACTTTC AACAGAGGAC 
TGTCTTATTC CACTCTTCAG TGAAGCTTTA CGTTCATGTA AACAGCATGA CGTGAGGCCA 
TGGATGCAGG CATTAAGGTA TACTATGTAC CAGAATCAGT TGTTGGAGAA AATTAAAGAA 
CAAACAGTCC CAATTAGAAG CCATCTCATG GAATTAGGTC TAACAGCAGC AAAATTTGCT 
AGAAAACGAG GGAATGTGTC CCTTGCAACA AGACTGCTGG CACAGTGCAG TGAAGTTCAG 
CTGGGAAAGA CCACCACTGC ACAGGATTTA GTCCAACATT TTAAAAAACT ATCAACCCAA 
GGTCAAGTGG ATGAAAAATG GGGGCCCGAA CTTGATATTG AAAAAACCAA ATTGCTTTAT 
ACAGCAGGCC AGTCAACACA TGCAATGGAA ATGTTGAGTT CTTGTGCCAT ATCTTTCTGC 
AAGTCTGTGA AAGCTGAATA TGCAGTTGCT AAATCAATTC TGACACTGGC TAAATGGATC 
CAGGCAGAAT GGAAAGAGAT TTCAGGACAG CTGAAACAGG TTTACAGAGC TCAGCACCAA 
CAGAACTTCA CAGGTCTTTC TACTTTGTCT AAAAACATAC TCACTCTAAT AGAACTGCCA 
TCTGTTAATA CGATGGAAGA AGAGTATCCT CGGATCGAGA GTGAATCTAC AGTGCATATT 
GGAGTTGGAG AACCTGACTT CATTTTGGGA CAGTTGTATC ACCTGTCTTC AGTACAGGCA 
CCTGAAGTAG CCAAATCTTG GGCAGCGTTG GCCAGCTGGG CTTATAGGTG GGGCAGAAAG 
GTGGTTGACA ATGCCAGTCA GGGAGAAGGT GTTCGTCTGC TGCCTAGAGA AAAATCTGAA 
GTTCAGAATC TACTTCCAGA CACTATAACT GAGGAAGAGA AAGAGAGAAT ATATGGTATT 
CTTGGACAGG CTGTGTGTCG GCCGGCGGGG ATTCAGGATG AAGATATAAC ACTTCAGATA 
ACTGAGAGTG AAGACAACGA AGAAGATGAC ATGGTTGATG TTATCTGGCG TCAGTTGATA 
TCAAGCTGCC CATGGCTTTC AGAACTTGAT GAAAGTGCAA CTGAAGGAGT TATTAAAGTG 
TGGAGGAAAG TTGTAGATAG AATATTCAGC CTGTACAAAC TCTCTTGCAG TGCATACTTT 
ACTTTCCTTA AACTCAACGC TGGTCAAATT CCTTTAGATG AGGATGACCC TAGGCTGCAT 
TTAAGTCACA GAGTGGAACA GAGCACTGAT GACATGATTG TGATGGCCAC ATTGCGCCTG 
CTGCGGTTGC TCGTGAAGCA TGCTGGTGAG CTTCGGCAGT ATCTGGAGCA CGGCTTGGAG 
ACAACACCCA CTGCACCATG GAGAGGAATT ATTCCGCAAC TTTTCTCACG CTTAAACCAC 
CCTGAAGTGT ATGTGCGCCA AAGTATTTGT AACCTTCTCT GCCGTGTGGC TCAAGATTCC 
CCACATCTCA TATTGTATCC TGCAATAGTG GGTACCATAT CGCTTAGTAG TGAATCCCAG 
GCTTCAGGAA ATAAATTTTC CACTGCAATT CCAACTTTAC TTGGCAATAT TCAAGGAGAA 
GAATTGCTGG TTTCTGAATG TGAGGGAGGA AGTCCTCCTG CATCTCAGGA TAGCAATAAG 
GATGAACCTA AAAGTGGATT AAATGAAGAC CAAGCCATGA TGCAGGATTG TTACAGCAAA 
ATTGTAGATA AGCTGTCCTC TGCAAACCCC ACCATGGTAT TACAGGTTCA GATGCTCGTG 
GCTGAACTGC GCAGGGTCAC TGTGCTCTGG GATGAGCTCT GGCTGGGAGT TTTGCTGCAA 
CAACACATGT ATGTCCTGAG ACGAATTCAG CAGCTTGAAG ATGAGGTGAA GAGAGTCCAG 
AACAACAACA CCTTACGCAA AGAAGAGAAA ATTGCAATCA TGAGGGAGAA GCACACAGCT 
TTGATGAAGC CCATCGTATT TGCTTTGGAG CATGTGAGGA GTATCACAGC GGCTCCTGCA 
GAAACACCTC ATGAAAAATG GTTTCAGGAT AACTATGGTG ATGCCATTGA AAATGCCCTA 
GAAAAACTGA AGACTCCATT GAACCCTGCA AAGCCTGGGA GCAGCTGGAT TCCATTTAAA 
GAGATAATGC TAAGTTTGCA ACAGAGAGCA CAGAAACGTG CAAGTTACAT CTTGCGTCTT 
GAAGAAATCA GTCCATGGTT GGCTGCCATG ACTAACACTG AAATTGCTCT TCCTGGGGAA 
GTCTCAGCCA GAGACACTGT CACAATCCAT AGTGTGGGCG GAACCATCAC AATCTTACCG 
ACTAAAACCA AGCCAAAGAA ACTTCTCTTT CTTGGATCAG ATGGGAAGAG CTATCCTTAT 
CTTTTCAAAG GACTGGAGGA TTTACATCTG GATGAGAGAA TAATGCAGTT CCTATCTATT 
GTGAATACCA TGTTTGCTAC AATTAATCGC CAAGAAACAC CCCGGTTCCA TGCTCGACAC 
TATTCTGTAA CACCACTAGG AACAAGATCA GGACTAATCC AGTGGGTAGA TGGAGCCACA 
CCCTTATTTG GTCTTTACAA ACGATGGCAA CAACGGGAAG CTGCCTTACA AGCACAAAAG 
GCCCAAGATT CCTACCAAAC TCCTCAGAAT CCTGGAATTG TACCCCGTCC TAGTGAACTT 
TATTACAGTA AAATTGGCCC TGCTTTGAAA ACAGTTGGGC TTAGCCTGGA TGTGTCCCGT 
CGGGATTGGC CTCTTCATGT AATGAAGGCA GTATTGGAAG AGTTAATGGA GGCCACACCC 
CCGAATCTCC TTGCCAAAGA GCTCTGGTCA TCTTGCACAA CACCTGATGA ATGGTGGAGA 
GTTACGCAGT CTTATGCAAG ATCTACTGCA GTCATGTCTA TGGTTGGATA CATAATTGGC 
CTTGGAGACA GACATCTGGA TAATGTTCTT ATAGATATGA CGACTGGAGA AGTTGTTCAC 
ATAGATTACA ATGTTTGCTT TGAAAAAGGT AATATAAAAG TAGACTTTAC ATTTATGAGG 
GCCAAATGTT TTTTGGTGAA AGGGCAAAAA TATAATGATT TGTCTTTTCA AGGTAAAAGC 
CTTAGAGTTC CTGAGAAAGT ACCTTTTCGA ATGACACAAA ACATTGAAAC AGCACTGGGT 
GTAACTGGAG TAGAAGGTGT ATTTAGGCTT TCATGTGAGC AGGTTTTACA CATTATGCGG 
CGTGGCAGAG AGACCCTGCT GACGCTGCTG GAGGCCTTTG TGTACGACCC TCTGGTGGAC 
TGGACAGCAG GAGGCGAGGC TGGGTTTGCT GGTGCTGTCT ATGGTGGAGG TGGCCAGCAG 
GCCGAGAGCA AGCAGAGCAA GAGAGAGATG GAGCGAGAGA TCACCCGCAG CCTGTTTTCT 
TCTAGAGTAG CTGAGATTAA GGTGAACTGG TTTAAGAATA GAGATGAGAT GCTGGTTGTG 
CTTCCCAAGT TGGACGGTAG CTTAGATGAA TACCTAAGCT TGCAAGAGCA ACTGACAGAT 
GTGGAAAAAC TGCAGGGCAA ACTACTGGAG GAAATAGAGT TTCTAGAAGG AGCTGAAGGG 
GTGGATCATC CTTCTCATAC TCTGCAACAC AGGTATTCTG AGCACACCCA ACTACAGACT 
CAGCAAAGAG CTGTTCAGGA AGCAATCCAG GTGAAGCTGA ATGAATTTGA ACAATGGATA 
ACACATTATC AGGCTGCATT CAATAATTTA GAAGCAACAC AGCTTGCAAG CTTGCTTCAA 
GAGATAAGCA CACAAATGGA CCTTGGTCCT CCAAGTTACG TGCCAGCAAC AGCCTTTCTG 
CAGAATGCTG GTCAGGCCCA CTTGATTAGC CAGTGCGAGC AGCTGGAGGG GGAGGTTGGT 
GCTCTCCTGC AGCAGAGGCG CTCCGTGCTC CGTGGCTGTC TGGAGCAACT GCATCACTAT 
GCAACCGTGG CCCTGCAGTA TCCGAAGGCC ATATTTCAGA AACATCGAAT TGAACAGTGG 
AAGACCTGGA TGGAAGAGCT CATCTGTAAC ACCACAGTAG AGCGTTGTCA AGAGCTCTAT 
AGGAAATATG AAATGCAATA TGCTCCCCAG CCACCCCCAA CAGTGTGTCA GTTCATCACT 
GCCACTGAAA TGACCCTGCA GCGATACGCA GCAGACATCA ACAGCAGACT TATTAGACAA 
GTGGAACGCT TGAAACAGGA AGCTGTCACT GTGCCAGTTT GTGAAGATCA GTTGAAAGAA 
ATTGAACGTT GCATTAAAGT TTTCCTTCAT GAGAATGGAG AAGAAGGATC TTTGAGTCTA 
GCAAGTGTTA TTATTTCTGC CCTTTGTACC CTTACAAGGC GTAACCTGAT GATGGAAGGT 
GCAGCGTCAA GTGCTGGAGA ACAGCTGGTT GATCTGACTT CTCGGGATGG AGCCTGGTTC 
TTGGAGGAAC TCTGCAGTAT GAGCGGAAAC GTCACCTGCT TGGTTCAGTT ACTGAAGCAG 
TGCCACCTGG TGCCACAGGA CTTAGATATC CCGAACCCCA TGGAAGCGTC TGAGACAGTT 
CACTTAGCCA ATGGAGTGTA TACCTCACTT CAGGAATTGA ATTCGAATTT CCGGCAAATC 
ATATTTCCAG AAGCACTTCG ATGTTTAATG AAAGGGGAAT ACACGTTAGA AAGTATGCTG 
CATGAACTGG ACGGTCTTAT TGAGCAGACC ACCGATGGCG TTCCCCTGCA GACTCTAGTG 
GAATCTCTTC AGGCCTACTT AAGAAACGCA GCTATGGGAC TGGAAGAAGA AACACATGCT 
CATTACATCG ATGTTGCCAG ACTACTACAT GCTCAGTACG GTGAATTAAT CCAACCGAGA 
AATGGTTCAG TTGATGAAAC ACCCAAAATG TCAGCTGGCC AGATGCTTTT GGTAGCATTC 
GATGGCATGT TTGCTCAAGT TGAAACTGCT TTCAGCTTAT TAGTTGAAAA GTTGAACAAG 
ATGGAAATTC CCATAGCTTG GCGAAAGATT GACATCATAA GGGAAGCCAG GAGTACTCAA 
GTTAATTTTT TTGATGATGA TAATCACCGG CAGGTGCTAG AAGAGATTTT CTTTCTAAAA 
AGACTACAGA CTATTAAGGA GTTCTTCAGG CTCTGTGGTA CCTTTTCTAA AACATTGTCA 
GGATCAAGTT CACTTGAAGA TCAGAATACT GTGAATGGGC CTGTACAGAT TGTCAATGTG 
AAAACCCTTT TTAGAAACTC TTGTTTCAGT GAAGACCAAA TGGCCAAACC TATCAAGGCA 
TTCACAGCTG ACTTTGTGAG GCAGCTCTTG ATAGGGCTAC CCAACCAAGC CCTCGGACTC 
ACACTGTGCA GTTTTATCAG TGCTCTGGGT GTAGACATCA TTGCTCAAGT AGAGGCAAAG 
GACTTTGGTG CCGAAAGCAA AGTTTCTGTT GATGATCTCT GTAAGAAAGC GGTGGAACAT 
AACATCCAGA TAGGGAAGTT CTCTCAGCTG GTTATGAACA GGGCAACTGT GTTAGCAAGT 
TCTTACGACA CTGCCTGGAA GAAGCATGAC TTGGTGCGAA GGCTAGAAAC CAGTATTTCT 
TCTTGTAAGA CAAGCCTGCA GCGGGTTCAG CTGCATATTG CCATGTTTCA GTGGCAACAT 
GAAGATCTAC TTATCAATAG ACCACAAGCC ATGTCAGTCA CACCTCCCCC ACGGTCTGCT 
ATCCTAACCA GCATGAAAAA GAAGCTGCAT ACCCTGAGCC AGATTGAAAC TTCTATTGCA 
ACAGTTCAGG AGAAGCTAGC TGCACTTGAA TCAAGTATTG AACAGCGACT CAAGTGGGCA 
GGTGGTGCCA ACCCTGCATT GGCCCCTGTA CTACAAGATT TTGAAGCAAC GATAGCTGAA 
AGAAGAAATC TTGTCCTTAA AGAGAGCCAA AGAGCAAGTC AGGTCACATT TCTCTGCAGC 
AATATCATTC ATTTTGAAAG TTTACGAACA AGAACTGCAG AAGCCTTAAA CCTGGATGCG 
GCGTTATTTG AACTAATCAA GCGATGTCAG CAGATGTGTT CGTTTGCATC ACAGTTTAAC 
AGTTCAGTGT CTGAGTTAGA GCTTCGTTTA TTACAGAGAG TGGACACTGG TCTTGAACAT 
CCTATTGGCA GCTCTGAATG GCTTTTGTCA GCACACAAAC AGTTGACCCA GGATATGTCT 
ACTCAGAGGG CAATTCAGAC AGAGAAAGAG CAGCAGATAG AAACGGTCTG TGAAACAATT 
CAGAATCTGG TTGATAATAT AAAGACTGTG CTCACTGGTC ATAACCGACA GCTTGGAGAT 
GTCAAACATC TCTTGAAAGC TATGGCTAAG GATGAAGAAG CTGCTCTGGC AGATGGTGAA 
GATGTTCCCT ATGAGAACAG TGTTAGGCAG TTTTTGGGTG AATATAAATC ATGGCAAGAC 
AACATTCAAA CAGTTCTATT TACATTAGTC CAGGCTATGG GTCAGGTTCG AAGTCAAGAA 
CACGTTGAAA TGCTCCAGGA AATCACTCCC ACCTTGAAAG AACTGAAAAC ACAAAGTCAG 
AGTATCTATA ATAATTTAGT GAGTTTTGCA TCACCCTTAG TCACCGATGC AACAAATGAA 
TGTTCGAGTC CAACGTCATC TGCTACTTAT CAGCCATCCT TCGCTGCAGC AGTCCGGAGT 
AACACTGGCC AGAAGACTCA GCCTGATGTC ATGTCACAGA ATGCTAGAAA GCTGATCCAA 
AAAAATCTTG CTACATCAGC TGATACTCCA CCAAGCACCG TTCCAGGAAC TGGCAAGAGT 
GTTGCTTGTA GTCCTAAAAA GGCAGTCAGA GACCCTAAAA CTGGGAAAGC GGTGCAAGAG 
AGAAACTCCT ATGCAGTGAG TGTGTGGAAG AGAGTGAAAG CCAAGTTAGA GGGCCGAGAT 
GTTGATCCGA ATAGGAGGAT GTCAGTTGCT GAACAGGTTG ACTATGTCAT TAAGGAAGCA 
ACTAATCTAG ATAACTTGGC TCAGCTGTAT GAAGGTTGGA CAGCCTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_005255184.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005255241.1 Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X5, mRNA.

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CloneID	OHu49090
Clone ID Related Accession (Same CDS sequence)	XM_005255184.3 , XM_005255184.4
Accession Version	XM_005255184.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005255184.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGAGAG ATCGATGTCT TATTCTGATG AGTCTCGACT GTCGAATCTT 
CTTCGGAGGA TCACCCGGGA AGACGACAGA GACCGAAGAT TGGCTACTGT AAAGCAGTTG 
AAAGAATTTA TTCAGCAACC AGAAAATAAG CTGGTACTAG TTAAACAATT GGATAATATC 
TTGGCTGCTG TACATGACGT GCTTAATGAA AGTAGCAAAT TGCTTCAGGA GTTGAGACAG 
GAGGGAGCTT GCTGTCTTGG CCTTCTTTGT GCTTCTCTGA GCTATGAGGC TGAGAAGATC 
TTCAAGTGGA TTTTTAGCAA ATTTAGCTCA TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC 
TTATGTGCCA CCTACAAAGC ACTAGAGACT GTAGGAGAAA AGAAAGCCTT TTCATCTGTA 
ATGCAGCTTG TAATGACCAG CCTGCAGTCT ATTCTTGAAA ATGTGGATAC ACCAGAATTG 
CTTTGTAAAT GTGTTAAGTG CATTCTTTTG GTGGCTCGAT GTTACCCTCA TATTTTCAGC 
ACTAATTTTA GGGATACAGT TGATATATTA GTTGGATGGC ATATAGATCA TACTCAGAAA 
CCTTCGCTCA CGCAGCAGGT ATCTGGGTGG TTGCAGAGTT TGGAGCCATT TTGGGTAGCT 
GATCTTGCAT TTTCTACTAC TCTTCTTGGT CAGTTTCTGG AAGACATGGA AGCATATGCT 
GAGGACCTCA GCCATGTGGC CTCTGGGGAA TCAGTGGATG AAGATGTCCC TCCTCCATCA 
GTGTCATTAC CAAAGCTGGC TGCACTTCTC CGGGTATTTA GTACTGTGGT GAGGAGCATT 
GGGGAACGCT TCAGCCCAAT TCGGGGTCCT CCAATTACTG AGGCATATGT AACAGATGTT 
CTGTACAGAG TAATGAGATG TGTGACGGCT GCAAACCAGG TGTTTTTTTC TGAGGCTGTG 
TTGACAGCTG CTAATGAGTG TGTTGGTGTT TTGCTCGGCA GCTTGGATCC TAGCATGACT 
ATACATTGTG ACATGGTCAT TACATATGGA TTAGACCAAC TGGAGAATTG CCAGACTTGT 
GGTACCGATT ATATCATCTC AGTCTTGAAT TTACTCACGC TGATTGTTGA ACAGATAAAT 
ACGAAACTGC CATCATCATT TGTAGAAAAA CTGTTTATAC CATCATCTAA ACTACTATTC 
TTGCGTTATC ATAAAGAAAA AGAGGTTGTT GCTGTAGCCC ATGCTGTTTA TCAAGCAGTG 
CTCAGCTTGA AGAATATTCC TGTTTTGGAG ACTGCCTATA AGTTAATATT GGGAGAAATG 
ACTTGTGCCC TAAACAACCT CCTACACAGT CTACAACTTC CTGAGGCCTG TTCTGAAATA 
AAACATGAGG CTTTTAAGAA TCATGTGTTC AATGTAGACA ATGCAAAATT TGTAGTTATA 
TTTGACCTCA GTGCCCTGAC TACAATTGGA AATGCCAAAA ACTCACTAAT AGGGATGTGG 
GCGCTATCTC CAACTGTCTT TGCACTTCTG AGTAAGAATC TGATGATTGT GCACAGTGAC 
CTGGCTGTTC ACTTCCCTGC CATTCAGTAT GCTGTGCTCT ACACATTGTA TTCTCATTGT 
ACCAGGCATG ATCACTTTAT CTCTAGTAGC CTCAGTTCTT CCTCTCCTTC TTTGTTTGAT 
GGAGCTGTGA TTAGCACTGT AACTACGGCT ACAAAGAAAC ATTTCTCAAT TATATTAAAT 
CTTCTGGGAA TATTACTTAA GAAAGATAAC CTTAACCAGG ACACGAGGAA ACTGTTAATG 
ACTTGGGCTT TGGAAGCAGC TGTTTTAATG AAGAAGTCTG AAACATACGC ACCTTTATTC 
TCTCTTCCGT CTTTCCATAA ATTTTGCAAA GGCCTTTTAG CCAACACTCT CGTTGAAGAT 
GTGAATATCT GTCTGCAGGC ATGCAGCAGT CTACATGCTC TGTCCTCTTC CTTGCCAGAT 
GATCTTTTAC AGAGATGTGT CGATGTTTGC CGTGTTCAAC TAGTGCACAG TGGAACTCGT 
ATTCGACAAG CATTTGGAAA ACTGTTGAAA TCAATTCCTT TAGATGTTGT CCTAAGCAAT 
AACAATCACA CAGAAATTCA AGAAATTTCT TTAGCATTAA GAAGTCACAT GAGTAAAGCA 
CCAAGTAATA CATTCCACCC CCAAGATTTC TCTGATGTTA TTAGTTTTAT TTTGTATGGG 
AACTCTCATA GAACAGGGAA GGACAATTGG TTGGAAAGAC TGTTCTATAG CTGCCAGAGA 
CTGGATAAGC GTGACCAGTC AACAATTCCA CGCAATCTCC TGAAGACAGA TGCTGTCCTT 
TGGCAGTGGG CCATATGGGA AGCTGCACAA TTCACTGTTC TTTCTAAGCT GAGAACCCCA 
CTGGGCAGAG CTCAAGACAC CTTCCAGACA ATTGAAGGTA TCATTCGAAG TCTCGCAGCT 
CACACATTAA ACCCTGATCA GGATGTTAGT CAGTGGACAA CTGCAGACAA TGATGAAGGC 
CATGGTAACA ACCAACTTAG ACTTGTTCTT CTTCTGCAGT ATCTGGAAAA TCTGGAGAAA 
TTAATGTATA ATGCATACGA GGGATGTGCT AATGCATTAA CTTCACCTCC CAAGGTCATT 
AGAACTTTTT TCTATACCAA TCGCCAAACT TGTCAGGACT GGCTAACGCG GATTCGACTC 
TCCATCATGA GGGTAGGATT GTTGGCAGGC CAGCCTGCAG TGACAGTGAG ACATGGCTTT 
GACTTGCTTA CAGAGATGAA AACAACCAGC CTATCTCAGG GGAATGAATT GGAAGTAACC 
ATTATGATGG TGGTAGAAGC ATTATGTGAA CTTCATTGTC CTGAAGCTAT ACAGGGAATT 
GCTGTCTGGT CATCATCTAT TGTTGGAAAA AATCTTCTGT GGATTAACTC AGTGGCTCAA 
CAGGCTGAAG GGAGGTTTGA AAAGGCCTCT GTGGAGTACC AGGAACACCT GTGTGCCATG 
ACAGGTGTTG ATTGCTGCAT CTCCAGCTTT GACAAATCGG TGCTCACCTT AGCCAATGCT 
GGGCGTAACA GTGCCAGCCC GAAACATTCT CTGAATGGTG AATCCAGAAA AACTGTGCTG 
TCCAAACCGA CTGACTCTTC CCCTGAGGTT ATAAATTATT TAGGAAATAA AGCATGTGAG 
TGCTACATCT CAATTGCCGA TTGGGCTGCT GTGCAGGAAT GGCAGAACGC TATCCATGAC 
TTGAAAAAGA GTACCAGTAG CACTTCCCTC AACCTGAAAG CTGACTTCAA CTATATAAAA 
TCATTAAGCA GCTTTGAGTC TGGAAAATTT GTTGAATGTA CCGAGCAGTT AGAATTGTTA 
CCAGGAGAAA ATATCAATCT ACTTGCTGGA GGATCAAAAG AAAAAATAGA CATGAAAAAA 
CTGCTTCCTA ACATGTTAAG TCCGGATCCG AGGGAACTTC AGAAATCCAT TGAAGTTCAA 
TTGTTAAGAA GTTCTGTTTG TTTGGCAACT GCTTTAAACC CGATAGAACA AGATCAGAAG 
TGGCAGTCTA TAACTGAAAA TGTGGTAAAG TACTTGAAGC AAACATCCCG CATCGCTATT 
GGACCTCTGA GACTTTCTAC TTTAACAGTT TCACAGTCTT TGCCAGTTCT AAGTACCTTG 
CAGCTGTATT GCTCATCTGC TTTGGAGAAC ACAGTTTCTA ACAGACTTTC AACAGAGGAC 
TGTCTTATTC CACTCTTCAG TGAAGCTTTA CGTTCATGTA AACAGCATGA CGTGAGGCCA 
TGGATGCAGG CATTAAGGTA TACTATGTAC CAGAATCAGT TGTTGGAGAA AATTAAAGAA 
CAAACAGTCC CAATTAGAAG CCATCTCATG GAATTAGGTC TAACAGCAGC AAAATTTGCT 
AGAAAACGAG GGAATGTGTC CCTTGCAACA AGACTGCTGG CACAGTGCAG TGAAGTTCAG 
CTGGGAAAGA CCACCACTGC ACAGGATTTA GTCCAACATT TTAAAAAACT ATCAACCCAA 
GGTCAAGTGG ATGAAAAATG GGGGCCCGAA CTTGATATTG AAAAAACCAA ATTGCTTTAT 
ACAGCAGGCC AGTCAACACA TGCAATGGAA ATGTTGAGTT CTTGTGCCAT ATCTTTCTGC 
AAGTCTGTGA AAGCTGAATA TGCAGTTGCT AAATCAATTC TGACACTGGC TAAATGGATC 
CAGGCAGAAT GGAAAGAGAT TTCAGGACAG CTGAAACAGG TTTACAGAGC TCAGCACCAA 
CAGAACTTCA CAGGTCTTTC TACTTTGTCT AAAAACATAC TCACTCTAAT AGAACTGCCA 
TCTGTTAATA CGATGGAAGA AGAGTATCCT CGGATCGAGA GTGAATCTAC AGTGCATATT 
GGAGTTGGAG AACCTGACTT CATTTTGGGA CAGTTGTATC ACCTGTCTTC AGTACAGGCA 
CCTGAAGTAG CCAAATCTTG GGCAGCGTTG GCCAGCTGGG CTTATAGGTG GGGCAGAAAG 
GTGGTTGACA ATGCCAGTCA GGGAGAAGGT GTTCGTCTGC TGCCTAGAGA AAAATCTGAA 
GTTCAGAATC TACTTCCAGA CACTATAACT GAGGAAGAGA AAGAGAGAAT ATATGGTATT 
CTTGGACAGG CTGTGTGTCG GCCGGCGGGG ATTCAGGATG AAGATATAAC ACTTCAGATA 
ACTGAGAGTG AAGACAACGA AGAAGATGAC ATGGTTGATG TTATCTGGCG TCAGTTGATA 
TCAAGCTGCC CATGGCTTTC AGAACTTGAT GAAAGTGCAA CTGAAGGAGT TATTAAAGTG 
TGGAGGAAAG TTGTAGATAG AATATTCAGC CTGTACAAAC TCTCTTGCAG TGCATACTTT 
ACTTTCCTTA AACTCAACGC TGGTCAAATT CCTTTAGATG AGGATGACCC TAGGCTGCAT 
TTAAGTCACA GAGTGGAACA GAGCACTGAT GACATGATTG TGATGGCCAC ATTGCGCCTG 
CTGCGGTTGC TCGTGAAGCA TGCTGGTGAG CTTCGGCAGT ATCTGGAGCA CGGCTTGGAG 
ACAACACCCA CTGCACCATG GAGAGGAATT ATTCCGCAAC TTTTCTCACG CTTAAACCAC 
CCTGAAGTGT ATGTGCGCCA AAGTATTTGT AACCTTCTCT GCCGTGTGGC TCAAGATTCC 
CCACATCTCA TATTGTATCC TGCAATAGTG GGTACCATAT CGCTTAGTAG TGAATCCCAG 
GCTTCAGGAA ATAAATTTTC CACTGCAATT CCAACTTTAC TTGGCAATAT TCAAGGAGAA 
GAATTGCTGG TTTCTGAATG TGAGGGAGGA AGTCCTCCTG CATCTCAGGA TAGCAATAAG 
GATGAACCTA AAAGTGGATT AAATGAAGAC CAAGCCATGA TGCAGGATTG TTACAGCAAA 
ATTGTAGATA AGCTGTCCTC TGCAAACCCC ACCATGGTAT TACAGGTTCA GATGCTCGTG 
GCTGAACTGC GCAGGGTCAC TGTGCTCTGG GATGAGCTCT GGCTGGGAGT TTTGCTGCAA 
CAACACATGT ATGTCCTGAG ACGAATTCAG CAGCTTGAAG ATGAGGTGAA GAGAGTCCAG 
AACAACAACA CCTTACGCAA AGAAGAGAAA ATTGCAATCA TGAGGGAGAA GCACACAGCT 
TTGATGAAGC CCATCGTATT TGCTTTGGAG CATGTGAGGA GTATCACAGC GGCTCCTGCA 
GAAACACCTC ATGAAAAATG GTTTCAGGAT AACTATGGTG ATGCCATTGA AAATGCCCTA 
GAAAAACTGA AGACTCCATT GAACCCTGCA AAGCCTGGGA GCAGCTGGAT TCCATTTAAA 
GAGATAATGC TAAGTTTGCA ACAGAGAGCA CAGAAACGTG CAAGTTACAT CTTGCGTCTT 
GAAGAAATCA GTCCATGGTT GGCTGCCATG ACTAACACTG AAATTGCTCT TCCTGGGGAA 
GTCTCAGCCA GAGACACTGT CACAATCCAT AGTGTGGGCG GAACCATCAC AATCTTACCG 
ACTAAAACCA AGCCAAAGAA ACTTCTCTTT CTTGGATCAG ATGGGAAGAG CTATCCTTAT 
CTTTTCAAAG GACTGGAGGA TTTACATCTG GATGAGAGAA TAATGCAGTT CCTATCTATT 
GTGAATACCA TGTTTGCTAC AATTAATCGC CAAGAAACAC CCCGGTTCCA TGCTCGACAC 
TATTCTGTAA CACCACTAGG AACAAGATCA GGACTAATCC AGTGGGTAGA TGGAGCCACA 
CCCTTATTTG GTCTTTACAA ACGATGGCAA CAACGGGAAG CTGCCTTACA AGCACAAAAG 
GCCCAAGATT CCTACCAAAC TCCTCAGAAT CCTGGAATTG TACCCCGTCC TAGTGAACTT 
TATTACAGTA AAATTGGCCC TGCTTTGAAA ACAGTTGGGC TTAGCCTGGA TGTGTCCCGT 
CGGGATTGGC CTCTTCATGT AATGAAGGCA GTATTGGAAG AGTTAATGGA GGCCACACCC 
CCGAATCTCC TTGCCAAAGA GCTCTGGTCA TCTTGCACAA CACCTGATGA ATGGTGGAGA 
GTTACGCAGT CTTATGCAAG ATCTACTGCA GTCATGTCTA TGGTTGGATA CATAATTGGC 
CTTGGAGACA GACATCTGGA TAATGTTCTT ATAGATATGA CGACTGGAGA AGTTGTTCAC 
ATAGATTACA ATGTTTGCTT TGAAAAAGGT AATATAAAAG TAGACTTTAC ATTTATGAGG 
GCCAAATGTT TTTTGGTGAA AGGGCAAAAA TATAATGATT TGTCTTTTCA AGGTAAAAGC 
CTTAGAGTTC CTGAGAAAGT ACCTTTTCGA ATGACACAAA ACATTGAAAC AGCACTGGGT 
GTAACTGGAG TAGAAGGTGT ATTTAGGCTT TCATGTGAGC AGGTTTTACA CATTATGCGG 
CGTGGCAGAG AGACCCTGCT GACGCTGCTG GAGGCCTTTG TGTACGACCC TCTGGTGGAC 
TGGACAGCAG GAGGCGAGGC TGGGTTTGCT GGTGCTGTCT ATGGTGGAGG TGGCCAGCAG 
GCCGAGAGCA AGCAGAGCAA GAGAGAGATG GAGCGAGAGA TCACCCGCAG CCTGTTTTCT 
TCTAGAGTAG CTGAGATTAA GGTGAACTGG TTTAAGAATA GAGATGAGAT GCTGGTTGTG 
CTTCCCAAGT TGGACGGTAG CTTAGATGAA TACCTAAGCT TGCAAGAGCA ACTGACAGAT 
GTGGAAAAAC TGCAGGGCAA ACTACTGGAG GAAATAGAGT TTCTAGAAGG AGCTGAAGGG 
GTGGATCATC CTTCTCATAC TCTGCAACAC AGGTATTCTG AGCACACCCA ACTACAGACT 
CAGCAAAGAG CTGTTCAGGA AGCAATCCAG GTGAAGCTGA ATGAATTTGA ACAATGGATA 
ACACATTATC AGGCTGCATT CAATAATTTA GAAGCAACAC AGCTTGCAAG CTTGCTTCAA 
GAGATAAGCA CACAAATGGA CCTTGGTCCT CCAAGTTACG TGCCAGCAAC AGCCTTTCTG 
CAGAATGCTG GTCAGGCCCA CTTGATTAGC CAGTGCGAGC AGCTGGAGGG GGAGGTTGGT 
GCTCTCCTGC AGCAGAGGCG CTCCGTGCTC CGTGGCTGTC TGGAGCAACT GCATCACTAT 
GCAACCGTGG CCCTGCAGTA TCCGAAGGCC ATATTTCAGA AACATCGAAT TGAACAGTGG 
AAGACCTGGA TGGAAGAGCT CATCTGTAAC ACCACAGTAG AGCGTTGTCA AGAGCTCTAT 
AGGAAATATG AAATGCAATA TGCTCCCCAG CCACCCCCAA CAGTGTGTCA GTTCATCACT 
GCCACTGAAA TGACCCTGCA GCGATACGCA GCAGACATCA ACAGCAGACT TATTAGACAA 
GTGGAACGCT TGAAACAGGA AGCTGTCACT GTGCCAGTTT GTGAAGATCA GTTGAAAGAA 
ATTGAACGTT GCATTAAAGT TTTCCTTCAT GAGAATGGAG AAGAAGGATC TTTGAGTCTA 
GCAAGTGTTA TTATTTCTGC CCTTTGTACC CTTACAAGGC GTAACCTGAT GATGGAAGGT 
GCAGCGTCAA GTGCTGGAGA ACAGCTGGTT GATCTGACTT CTCGGGATGG AGCCTGGTTC 
TTGGAGGAAC TCTGCAGTAT GAGCGGAAAC GTCACCTGCT TGGTTCAGTT ACTGAAGCAG 
TGCCACCTGG TGCCACAGGA CTTAGATATC CCGAACCCCA TGGAAGCGTC TGAGACAGTT 
CACTTAGCCA ATGGAGTGTA TACCTCACTT CAGGAATTGA ATTCGAATTT CCGGCAAATC 
ATATTTCCAG AAGCACTTCG ATGTTTAATG AAAGGGGAAT ACACGTTAGA AAGTATGCTG 
CATGAACTGG ACGGTCTTAT TGAGCAGACC ACCGATGGCG TTCCCCTGCA GACTCTAGTG 
GAATCTCTTC AGGCCTACTT AAGAAACGCA GCTATGGGAC TGGAAGAAGA AACACATGCT 
CATTACATCG ATGTTGCCAG ACTACTACAT GCTCAGTACG GTGAATTAAT CCAACCGAGA 
AATGGTTCAG TTGATGAAAC ACCCAAAATG TCAGCTGGCC AGATGCTTTT GGTAGCATTC 
GATGGCATGT TTGCTCAAGT TGAAACTGCT TTCAGCTTAT TAGTTGAAAA GTTGAACAAG 
ATGGAAATTC CCATAGCTTG GCGAAAGATT GACATCATAA GGGAAGCCAG GAGTACTCAA 
GTTAATTTTT TTGATGATGA TAATCACCGG CAGGTGCTAG AAGAGATTTT CTTTCTAAAA 
AGACTACAGA CTATTAAGGA GTTCTTCAGG CTCTGTGGTA CCTTTTCTAA AACATTGTCA 
GGATCAAGTT CACTTGAAGA TCAGAATACT GTGAATGGGC CTGTACAGAT TGTCAATGTG 
AAAACCCTTT TTAGAAACTC TTGTTTCAGT GAAGACCAAA TGGCCAAACC TATCAAGGCA 
TTCACAGCTG ACTTTGTGAG GCAGCTCTTG ATAGGGCTAC CCAACCAAGC CCTCGGACTC 
ACACTGTGCA GTTTTATCAG TGCTCTGGGT GTAGACATCA TTGCTCAAGT AGAGGCAAAG 
GACTTTGGTG CCGAAAGCAA AGTTTCTGTT GATGATCTCT GTAAGAAAGC GGTGGAACAT 
AACATCCAGA TAGGGAAGTT CTCTCAGCTG GTTATGAACA GGGCAACTGT GTTAGCAAGT 
TCTTACGACA CTGCCTGGAA GAAGCATGAC TTGGTGCGAA GGCTAGAAAC CAGTATTTCT 
TCTTGTAAGA CAAGCCTGCA GCGGGTTCAG CTGCATATTG CCATGTTTCA GTGGCAACAT 
GAAGATCTAC TTATCAATAG ACCACAAGCC ATGTCAGTCA CACCTCCCCC ACGGTCTGCT 
ATCCTAACCA GCATGAAAAA GAAGCTGCAT ACCCTGAGCC AGATTGAAAC TTCTATTGCA 
ACAGTTCAGG AGAAGCTAGC TGCACTTGAA TCAAGTATTG AACAGCGACT CAAGTGGGCA 
GGTGGTGCCA ACCCTGCATT GGCCCCTGTA CTACAAGATT TTGAAGCAAC GATAGCTGAA 
AGAAGAAATC TTGTCCTTAA AGAGAGCCAA AGAGCAAGTC AGGTCACATT TCTCTGCAGC 
AATATCATTC ATTTTGAAAG TTTACGAACA AGAACTGCAG AAGCCTTAAA CCTGGATGCG 
GCGTTATTTG AACTAATCAA GCGATGTCAG CAGATGTGTT CGTTTGCATC ACAGTTTAAC 
AGTTCAGTGT CTGAGTTAGA GCTTCGTTTA TTACAGAGAG TGGACACTGG TCTTGAACAT 
CCTATTGGCA GCTCTGAATG GCTTTTGTCA GCACACAAAC AGTTGACCCA GGATATGTCT 
ACTCAGAGGG CAATTCAGAC AGAGAAAGAG CAGCAGATAG AAACGGTCTG TGAAACAATT 
CAGAATCTGG TTGATAATAT AAAGACTGTG CTCACTGGTC ATAACCGACA GCTTGGAGAT 
GTCAAACATC TCTTGAAAGC TATGGCTAAG GATGAAGAAG CTGCTCTGGC AGATGGTGAA 
GATGTTCCCT ATGAGAACAG TGTTAGGCAG TTTTTGGGTG AATATAAATC ATGGCAAGAC 
AACATTCAAA CAGTTCTATT TACATTAGTC CAGGCTATGG GTCAGGTTCG AAGTCAAGAA 
CACGTTGAAA TGCTCCAGGA AATCACTCCC ACCTTGAAAG AACTGAAAAC ACAAAGTCAG 
AGTATCTATA ATAATTTAGT GAGTTTTGCA TCACCCTTAG TCACCGATGC AACAAATGAA 
TGTTCGAGTC CAACGTCATC TGCTACTTAT CAGCCATCCT TCGCTGCAGC AGTCCGGAGT 
AACACTGGCC AGAAGACTCA GCCTGATGTC ATGTCACAGA ATGCTAGAAA GCTGATCCAA 
AAAAATCTTG CTACATCAGC TGATACTCCA CCAAGCACCG TTCCAGGAAC TGGCAAGAGT 
GTTGCTTGTA GTCCTAAAAA GGCAGTCAGA GACCCTAAAA CTGGGAAAGC GGTGCAAGAG 
AGAAACTCCT ATGCAGTGAG TGTGTGGAAG AGAGTGAAAG CCAAGTTAGA GGGCCGAGAT 
GTTGATCCGA ATAGGAGGAT GTCAGTTGCT GAACAGGTTG ACTATGTCAT TAAGGAAGCA 
ACTAATCTAG ATAACTTGGC TCAGCTGTAT GAAGGTTGGA CAGCCTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005255241.1
CDS	378..11291
Translation	MSRRAPGSRLSSGGGGGGTKYPRSWNDWQPRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGNIKVDFTFMRAKCFLVKGQKYNDLSFQGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_005255184.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005255184.4

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGAGAG ATCGATGTCT TATTCTGATG AGTCTCGACT GTCGAATCTT 
CTTCGGAGGA TCACCCGGGA AGACGACAGA GACCGAAGAT TGGCTACTGT AAAGCAGTTG 
AAAGAATTTA TTCAGCAACC AGAAAATAAG CTGGTACTAG TTAAACAATT GGATAATATC 
TTGGCTGCTG TACATGACGT GCTTAATGAA AGTAGCAAAT TGCTTCAGGA GTTGAGACAG 
GAGGGAGCTT GCTGTCTTGG CCTTCTTTGT GCTTCTCTGA GCTATGAGGC TGAGAAGATC 
TTCAAGTGGA TTTTTAGCAA ATTTAGCTCA TCTGCAAAAG ATGAAGTTAA ACTCCTCTAC 
TTATGTGCCA CCTACAAAGC ACTAGAGACT GTAGGAGAAA AGAAAGCCTT TTCATCTGTA 
ATGCAGCTTG TAATGACCAG CCTGCAGTCT ATTCTTGAAA ATGTGGATAC ACCAGAATTG 
CTTTGTAAAT GTGTTAAGTG CATTCTTTTG GTGGCTCGAT GTTACCCTCA TATTTTCAGC 
ACTAATTTTA GGGATACAGT TGATATATTA GTTGGATGGC ATATAGATCA TACTCAGAAA 
CCTTCGCTCA CGCAGCAGGT ATCTGGGTGG TTGCAGAGTT TGGAGCCATT TTGGGTAGCT 
GATCTTGCAT TTTCTACTAC TCTTCTTGGT CAGTTTCTGG AAGACATGGA AGCATATGCT 
GAGGACCTCA GCCATGTGGC CTCTGGGGAA TCAGTGGATG AAGATGTCCC TCCTCCATCA 
GTGTCATTAC CAAAGCTGGC TGCACTTCTC CGGGTATTTA GTACTGTGGT GAGGAGCATT 
GGGGAACGCT TCAGCCCAAT TCGGGGTCCT CCAATTACTG AGGCATATGT AACAGATGTT 
CTGTACAGAG TAATGAGATG TGTGACGGCT GCAAACCAGG TGTTTTTTTC TGAGGCTGTG 
TTGACAGCTG CTAATGAGTG TGTTGGTGTT TTGCTCGGCA GCTTGGATCC TAGCATGACT 
ATACATTGTG ACATGGTCAT TACATATGGA TTAGACCAAC TGGAGAATTG CCAGACTTGT 
GGTACCGATT ATATCATCTC AGTCTTGAAT TTACTCACGC TGATTGTTGA ACAGATAAAT 
ACGAAACTGC CATCATCATT TGTAGAAAAA CTGTTTATAC CATCATCTAA ACTACTATTC 
TTGCGTTATC ATAAAGAAAA AGAGGTTGTT GCTGTAGCCC ATGCTGTTTA TCAAGCAGTG 
CTCAGCTTGA AGAATATTCC TGTTTTGGAG ACTGCCTATA AGTTAATATT GGGAGAAATG 
ACTTGTGCCC TAAACAACCT CCTACACAGT CTACAACTTC CTGAGGCCTG TTCTGAAATA 
AAACATGAGG CTTTTAAGAA TCATGTGTTC AATGTAGACA ATGCAAAATT TGTAGTTATA 
TTTGACCTCA GTGCCCTGAC TACAATTGGA AATGCCAAAA ACTCACTAAT AGGGATGTGG 
GCGCTATCTC CAACTGTCTT TGCACTTCTG AGTAAGAATC TGATGATTGT GCACAGTGAC 
CTGGCTGTTC ACTTCCCTGC CATTCAGTAT GCTGTGCTCT ACACATTGTA TTCTCATTGT 
ACCAGGCATG ATCACTTTAT CTCTAGTAGC CTCAGTTCTT CCTCTCCTTC TTTGTTTGAT 
GGAGCTGTGA TTAGCACTGT AACTACGGCT ACAAAGAAAC ATTTCTCAAT TATATTAAAT 
CTTCTGGGAA TATTACTTAA GAAAGATAAC CTTAACCAGG ACACGAGGAA ACTGTTAATG 
ACTTGGGCTT TGGAAGCAGC TGTTTTAATG AAGAAGTCTG AAACATACGC ACCTTTATTC 
TCTCTTCCGT CTTTCCATAA ATTTTGCAAA GGCCTTTTAG CCAACACTCT CGTTGAAGAT 
GTGAATATCT GTCTGCAGGC ATGCAGCAGT CTACATGCTC TGTCCTCTTC CTTGCCAGAT 
GATCTTTTAC AGAGATGTGT CGATGTTTGC CGTGTTCAAC TAGTGCACAG TGGAACTCGT 
ATTCGACAAG CATTTGGAAA ACTGTTGAAA TCAATTCCTT TAGATGTTGT CCTAAGCAAT 
AACAATCACA CAGAAATTCA AGAAATTTCT TTAGCATTAA GAAGTCACAT GAGTAAAGCA 
CCAAGTAATA CATTCCACCC CCAAGATTTC TCTGATGTTA TTAGTTTTAT TTTGTATGGG 
AACTCTCATA GAACAGGGAA GGACAATTGG TTGGAAAGAC TGTTCTATAG CTGCCAGAGA 
CTGGATAAGC GTGACCAGTC AACAATTCCA CGCAATCTCC TGAAGACAGA TGCTGTCCTT 
TGGCAGTGGG CCATATGGGA AGCTGCACAA TTCACTGTTC TTTCTAAGCT GAGAACCCCA 
CTGGGCAGAG CTCAAGACAC CTTCCAGACA ATTGAAGGTA TCATTCGAAG TCTCGCAGCT 
CACACATTAA ACCCTGATCA GGATGTTAGT CAGTGGACAA CTGCAGACAA TGATGAAGGC 
CATGGTAACA ACCAACTTAG ACTTGTTCTT CTTCTGCAGT ATCTGGAAAA TCTGGAGAAA 
TTAATGTATA ATGCATACGA GGGATGTGCT AATGCATTAA CTTCACCTCC CAAGGTCATT 
AGAACTTTTT TCTATACCAA TCGCCAAACT TGTCAGGACT GGCTAACGCG GATTCGACTC 
TCCATCATGA GGGTAGGATT GTTGGCAGGC CAGCCTGCAG TGACAGTGAG ACATGGCTTT 
GACTTGCTTA CAGAGATGAA AACAACCAGC CTATCTCAGG GGAATGAATT GGAAGTAACC 
ATTATGATGG TGGTAGAAGC ATTATGTGAA CTTCATTGTC CTGAAGCTAT ACAGGGAATT 
GCTGTCTGGT CATCATCTAT TGTTGGAAAA AATCTTCTGT GGATTAACTC AGTGGCTCAA 
CAGGCTGAAG GGAGGTTTGA AAAGGCCTCT GTGGAGTACC AGGAACACCT GTGTGCCATG 
ACAGGTGTTG ATTGCTGCAT CTCCAGCTTT GACAAATCGG TGCTCACCTT AGCCAATGCT 
GGGCGTAACA GTGCCAGCCC GAAACATTCT CTGAATGGTG AATCCAGAAA AACTGTGCTG 
TCCAAACCGA CTGACTCTTC CCCTGAGGTT ATAAATTATT TAGGAAATAA AGCATGTGAG 
TGCTACATCT CAATTGCCGA TTGGGCTGCT GTGCAGGAAT GGCAGAACGC TATCCATGAC 
TTGAAAAAGA GTACCAGTAG CACTTCCCTC AACCTGAAAG CTGACTTCAA CTATATAAAA 
TCATTAAGCA GCTTTGAGTC TGGAAAATTT GTTGAATGTA CCGAGCAGTT AGAATTGTTA 
CCAGGAGAAA ATATCAATCT ACTTGCTGGA GGATCAAAAG AAAAAATAGA CATGAAAAAA 
CTGCTTCCTA ACATGTTAAG TCCGGATCCG AGGGAACTTC AGAAATCCAT TGAAGTTCAA 
TTGTTAAGAA GTTCTGTTTG TTTGGCAACT GCTTTAAACC CGATAGAACA AGATCAGAAG 
TGGCAGTCTA TAACTGAAAA TGTGGTAAAG TACTTGAAGC AAACATCCCG CATCGCTATT 
GGACCTCTGA GACTTTCTAC TTTAACAGTT TCACAGTCTT TGCCAGTTCT AAGTACCTTG 
CAGCTGTATT GCTCATCTGC TTTGGAGAAC ACAGTTTCTA ACAGACTTTC AACAGAGGAC 
TGTCTTATTC CACTCTTCAG TGAAGCTTTA CGTTCATGTA AACAGCATGA CGTGAGGCCA 
TGGATGCAGG CATTAAGGTA TACTATGTAC CAGAATCAGT TGTTGGAGAA AATTAAAGAA 
CAAACAGTCC CAATTAGAAG CCATCTCATG GAATTAGGTC TAACAGCAGC AAAATTTGCT 
AGAAAACGAG GGAATGTGTC CCTTGCAACA AGACTGCTGG CACAGTGCAG TGAAGTTCAG 
CTGGGAAAGA CCACCACTGC ACAGGATTTA GTCCAACATT TTAAAAAACT ATCAACCCAA 
GGTCAAGTGG ATGAAAAATG GGGGCCCGAA CTTGATATTG AAAAAACCAA ATTGCTTTAT 
ACAGCAGGCC AGTCAACACA TGCAATGGAA ATGTTGAGTT CTTGTGCCAT ATCTTTCTGC 
AAGTCTGTGA AAGCTGAATA TGCAGTTGCT AAATCAATTC TGACACTGGC TAAATGGATC 
CAGGCAGAAT GGAAAGAGAT TTCAGGACAG CTGAAACAGG TTTACAGAGC TCAGCACCAA 
CAGAACTTCA CAGGTCTTTC TACTTTGTCT AAAAACATAC TCACTCTAAT AGAACTGCCA 
TCTGTTAATA CGATGGAAGA AGAGTATCCT CGGATCGAGA GTGAATCTAC AGTGCATATT 
GGAGTTGGAG AACCTGACTT CATTTTGGGA CAGTTGTATC ACCTGTCTTC AGTACAGGCA 
CCTGAAGTAG CCAAATCTTG GGCAGCGTTG GCCAGCTGGG CTTATAGGTG GGGCAGAAAG 
GTGGTTGACA ATGCCAGTCA GGGAGAAGGT GTTCGTCTGC TGCCTAGAGA AAAATCTGAA 
GTTCAGAATC TACTTCCAGA CACTATAACT GAGGAAGAGA AAGAGAGAAT ATATGGTATT 
CTTGGACAGG CTGTGTGTCG GCCGGCGGGG ATTCAGGATG AAGATATAAC ACTTCAGATA 
ACTGAGAGTG AAGACAACGA AGAAGATGAC ATGGTTGATG TTATCTGGCG TCAGTTGATA 
TCAAGCTGCC CATGGCTTTC AGAACTTGAT GAAAGTGCAA CTGAAGGAGT TATTAAAGTG 
TGGAGGAAAG TTGTAGATAG AATATTCAGC CTGTACAAAC TCTCTTGCAG TGCATACTTT 
ACTTTCCTTA AACTCAACGC TGGTCAAATT CCTTTAGATG AGGATGACCC TAGGCTGCAT 
TTAAGTCACA GAGTGGAACA GAGCACTGAT GACATGATTG TGATGGCCAC ATTGCGCCTG 
CTGCGGTTGC TCGTGAAGCA TGCTGGTGAG CTTCGGCAGT ATCTGGAGCA CGGCTTGGAG 
ACAACACCCA CTGCACCATG GAGAGGAATT ATTCCGCAAC TTTTCTCACG CTTAAACCAC 
CCTGAAGTGT ATGTGCGCCA AAGTATTTGT AACCTTCTCT GCCGTGTGGC TCAAGATTCC 
CCACATCTCA TATTGTATCC TGCAATAGTG GGTACCATAT CGCTTAGTAG TGAATCCCAG 
GCTTCAGGAA ATAAATTTTC CACTGCAATT CCAACTTTAC TTGGCAATAT TCAAGGAGAA 
GAATTGCTGG TTTCTGAATG TGAGGGAGGA AGTCCTCCTG CATCTCAGGA TAGCAATAAG 
GATGAACCTA AAAGTGGATT AAATGAAGAC CAAGCCATGA TGCAGGATTG TTACAGCAAA 
ATTGTAGATA AGCTGTCCTC TGCAAACCCC ACCATGGTAT TACAGGTTCA GATGCTCGTG 
GCTGAACTGC GCAGGGTCAC TGTGCTCTGG GATGAGCTCT GGCTGGGAGT TTTGCTGCAA 
CAACACATGT ATGTCCTGAG ACGAATTCAG CAGCTTGAAG ATGAGGTGAA GAGAGTCCAG 
AACAACAACA CCTTACGCAA AGAAGAGAAA ATTGCAATCA TGAGGGAGAA GCACACAGCT 
TTGATGAAGC CCATCGTATT TGCTTTGGAG CATGTGAGGA GTATCACAGC GGCTCCTGCA 
GAAACACCTC ATGAAAAATG GTTTCAGGAT AACTATGGTG ATGCCATTGA AAATGCCCTA 
GAAAAACTGA AGACTCCATT GAACCCTGCA AAGCCTGGGA GCAGCTGGAT TCCATTTAAA 
GAGATAATGC TAAGTTTGCA ACAGAGAGCA CAGAAACGTG CAAGTTACAT CTTGCGTCTT 
GAAGAAATCA GTCCATGGTT GGCTGCCATG ACTAACACTG AAATTGCTCT TCCTGGGGAA 
GTCTCAGCCA GAGACACTGT CACAATCCAT AGTGTGGGCG GAACCATCAC AATCTTACCG 
ACTAAAACCA AGCCAAAGAA ACTTCTCTTT CTTGGATCAG ATGGGAAGAG CTATCCTTAT 
CTTTTCAAAG GACTGGAGGA TTTACATCTG GATGAGAGAA TAATGCAGTT CCTATCTATT 
GTGAATACCA TGTTTGCTAC AATTAATCGC CAAGAAACAC CCCGGTTCCA TGCTCGACAC 
TATTCTGTAA CACCACTAGG AACAAGATCA GGACTAATCC AGTGGGTAGA TGGAGCCACA 
CCCTTATTTG GTCTTTACAA ACGATGGCAA CAACGGGAAG CTGCCTTACA AGCACAAAAG 
GCCCAAGATT CCTACCAAAC TCCTCAGAAT CCTGGAATTG TACCCCGTCC TAGTGAACTT 
TATTACAGTA AAATTGGCCC TGCTTTGAAA ACAGTTGGGC TTAGCCTGGA TGTGTCCCGT 
CGGGATTGGC CTCTTCATGT AATGAAGGCA GTATTGGAAG AGTTAATGGA GGCCACACCC 
CCGAATCTCC TTGCCAAAGA GCTCTGGTCA TCTTGCACAA CACCTGATGA ATGGTGGAGA 
GTTACGCAGT CTTATGCAAG ATCTACTGCA GTCATGTCTA TGGTTGGATA CATAATTGGC 
CTTGGAGACA GACATCTGGA TAATGTTCTT ATAGATATGA CGACTGGAGA AGTTGTTCAC 
ATAGATTACA ATGTTTGCTT TGAAAAAGGT AATATAAAAG TAGACTTTAC ATTTATGAGG 
GCCAAATGTT TTTTGGTGAA AGGGCAAAAA TATAATGATT TGTCTTTTCA AGGTAAAAGC 
CTTAGAGTTC CTGAGAAAGT ACCTTTTCGA ATGACACAAA ACATTGAAAC AGCACTGGGT 
GTAACTGGAG TAGAAGGTGT ATTTAGGCTT TCATGTGAGC AGGTTTTACA CATTATGCGG 
CGTGGCAGAG AGACCCTGCT GACGCTGCTG GAGGCCTTTG TGTACGACCC TCTGGTGGAC 
TGGACAGCAG GAGGCGAGGC TGGGTTTGCT GGTGCTGTCT ATGGTGGAGG TGGCCAGCAG 
GCCGAGAGCA AGCAGAGCAA GAGAGAGATG GAGCGAGAGA TCACCCGCAG CCTGTTTTCT 
TCTAGAGTAG CTGAGATTAA GGTGAACTGG TTTAAGAATA GAGATGAGAT GCTGGTTGTG 
CTTCCCAAGT TGGACGGTAG CTTAGATGAA TACCTAAGCT TGCAAGAGCA ACTGACAGAT 
GTGGAAAAAC TGCAGGGCAA ACTACTGGAG GAAATAGAGT TTCTAGAAGG AGCTGAAGGG 
GTGGATCATC CTTCTCATAC TCTGCAACAC AGGTATTCTG AGCACACCCA ACTACAGACT 
CAGCAAAGAG CTGTTCAGGA AGCAATCCAG GTGAAGCTGA ATGAATTTGA ACAATGGATA 
ACACATTATC AGGCTGCATT CAATAATTTA GAAGCAACAC AGCTTGCAAG CTTGCTTCAA 
GAGATAAGCA CACAAATGGA CCTTGGTCCT CCAAGTTACG TGCCAGCAAC AGCCTTTCTG 
CAGAATGCTG GTCAGGCCCA CTTGATTAGC CAGTGCGAGC AGCTGGAGGG GGAGGTTGGT 
GCTCTCCTGC AGCAGAGGCG CTCCGTGCTC CGTGGCTGTC TGGAGCAACT GCATCACTAT 
GCAACCGTGG CCCTGCAGTA TCCGAAGGCC ATATTTCAGA AACATCGAAT TGAACAGTGG 
AAGACCTGGA TGGAAGAGCT CATCTGTAAC ACCACAGTAG AGCGTTGTCA AGAGCTCTAT 
AGGAAATATG AAATGCAATA TGCTCCCCAG CCACCCCCAA CAGTGTGTCA GTTCATCACT 
GCCACTGAAA TGACCCTGCA GCGATACGCA GCAGACATCA ACAGCAGACT TATTAGACAA 
GTGGAACGCT TGAAACAGGA AGCTGTCACT GTGCCAGTTT GTGAAGATCA GTTGAAAGAA 
ATTGAACGTT GCATTAAAGT TTTCCTTCAT GAGAATGGAG AAGAAGGATC TTTGAGTCTA 
GCAAGTGTTA TTATTTCTGC CCTTTGTACC CTTACAAGGC GTAACCTGAT GATGGAAGGT 
GCAGCGTCAA GTGCTGGAGA ACAGCTGGTT GATCTGACTT CTCGGGATGG AGCCTGGTTC 
TTGGAGGAAC TCTGCAGTAT GAGCGGAAAC GTCACCTGCT TGGTTCAGTT ACTGAAGCAG 
TGCCACCTGG TGCCACAGGA CTTAGATATC CCGAACCCCA TGGAAGCGTC TGAGACAGTT 
CACTTAGCCA ATGGAGTGTA TACCTCACTT CAGGAATTGA ATTCGAATTT CCGGCAAATC 
ATATTTCCAG AAGCACTTCG ATGTTTAATG AAAGGGGAAT ACACGTTAGA AAGTATGCTG 
CATGAACTGG ACGGTCTTAT TGAGCAGACC ACCGATGGCG TTCCCCTGCA GACTCTAGTG 
GAATCTCTTC AGGCCTACTT AAGAAACGCA GCTATGGGAC TGGAAGAAGA AACACATGCT 
CATTACATCG ATGTTGCCAG ACTACTACAT GCTCAGTACG GTGAATTAAT CCAACCGAGA 
AATGGTTCAG TTGATGAAAC ACCCAAAATG TCAGCTGGCC AGATGCTTTT GGTAGCATTC 
GATGGCATGT TTGCTCAAGT TGAAACTGCT TTCAGCTTAT TAGTTGAAAA GTTGAACAAG 
ATGGAAATTC CCATAGCTTG GCGAAAGATT GACATCATAA GGGAAGCCAG GAGTACTCAA 
GTTAATTTTT TTGATGATGA TAATCACCGG CAGGTGCTAG AAGAGATTTT CTTTCTAAAA 
AGACTACAGA CTATTAAGGA GTTCTTCAGG CTCTGTGGTA CCTTTTCTAA AACATTGTCA 
GGATCAAGTT CACTTGAAGA TCAGAATACT GTGAATGGGC CTGTACAGAT TGTCAATGTG 
AAAACCCTTT TTAGAAACTC TTGTTTCAGT GAAGACCAAA TGGCCAAACC TATCAAGGCA 
TTCACAGCTG ACTTTGTGAG GCAGCTCTTG ATAGGGCTAC CCAACCAAGC CCTCGGACTC 
ACACTGTGCA GTTTTATCAG TGCTCTGGGT GTAGACATCA TTGCTCAAGT AGAGGCAAAG 
GACTTTGGTG CCGAAAGCAA AGTTTCTGTT GATGATCTCT GTAAGAAAGC GGTGGAACAT 
AACATCCAGA TAGGGAAGTT CTCTCAGCTG GTTATGAACA GGGCAACTGT GTTAGCAAGT 
TCTTACGACA CTGCCTGGAA GAAGCATGAC TTGGTGCGAA GGCTAGAAAC CAGTATTTCT 
TCTTGTAAGA CAAGCCTGCA GCGGGTTCAG CTGCATATTG CCATGTTTCA GTGGCAACAT 
GAAGATCTAC TTATCAATAG ACCACAAGCC ATGTCAGTCA CACCTCCCCC ACGGTCTGCT 
ATCCTAACCA GCATGAAAAA GAAGCTGCAT ACCCTGAGCC AGATTGAAAC TTCTATTGCA 
ACAGTTCAGG AGAAGCTAGC TGCACTTGAA TCAAGTATTG AACAGCGACT CAAGTGGGCA 
GGTGGTGCCA ACCCTGCATT GGCCCCTGTA CTACAAGATT TTGAAGCAAC GATAGCTGAA 
AGAAGAAATC TTGTCCTTAA AGAGAGCCAA AGAGCAAGTC AGGTCACATT TCTCTGCAGC 
AATATCATTC ATTTTGAAAG TTTACGAACA AGAACTGCAG AAGCCTTAAA CCTGGATGCG 
GCGTTATTTG AACTAATCAA GCGATGTCAG CAGATGTGTT CGTTTGCATC ACAGTTTAAC 
AGTTCAGTGT CTGAGTTAGA GCTTCGTTTA TTACAGAGAG TGGACACTGG TCTTGAACAT 
CCTATTGGCA GCTCTGAATG GCTTTTGTCA GCACACAAAC AGTTGACCCA GGATATGTCT 
ACTCAGAGGG CAATTCAGAC AGAGAAAGAG CAGCAGATAG AAACGGTCTG TGAAACAATT 
CAGAATCTGG TTGATAATAT AAAGACTGTG CTCACTGGTC ATAACCGACA GCTTGGAGAT 
GTCAAACATC TCTTGAAAGC TATGGCTAAG GATGAAGAAG CTGCTCTGGC AGATGGTGAA 
GATGTTCCCT ATGAGAACAG TGTTAGGCAG TTTTTGGGTG AATATAAATC ATGGCAAGAC 
AACATTCAAA CAGTTCTATT TACATTAGTC CAGGCTATGG GTCAGGTTCG AAGTCAAGAA 
CACGTTGAAA TGCTCCAGGA AATCACTCCC ACCTTGAAAG AACTGAAAAC ACAAAGTCAG 
AGTATCTATA ATAATTTAGT GAGTTTTGCA TCACCCTTAG TCACCGATGC AACAAATGAA 
TGTTCGAGTC CAACGTCATC TGCTACTTAT CAGCCATCCT TCGCTGCAGC AGTCCGGAGT 
AACACTGGCC AGAAGACTCA GCCTGATGTC ATGTCACAGA ATGCTAGAAA GCTGATCCAA 
AAAAATCTTG CTACATCAGC TGATACTCCA CCAAGCACCG TTCCAGGAAC TGGCAAGAGT 
GTTGCTTGTA GTCCTAAAAA GGCAGTCAGA GACCCTAAAA CTGGGAAAGC GGTGCAAGAG 
AGAAACTCCT ATGCAGTGAG TGTGTGGAAG AGAGTGAAAG CCAAGTTAGA GGGCCGAGAT 
GTTGATCCGA ATAGGAGGAT GTCAGTTGCT GAACAGGTTG ACTATGTCAT TAAGGAAGCA 
ACTAATCTAG ATAACTTGGC TCAGCTGTAT GAAGGTTGGA CAGCCTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_011545769.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544071.1 Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

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CloneID	OHu65357
Clone ID Related Accession (Same CDS sequence)	XM_011545769.3 , XM_011545769.2
Accession Version	XM_011545769.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545769.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGGGTT AACAATGTGA CCAGCCCTGA GTTCACCAGT 
GTTCAGCATG GCAGTCGTGC TTTAGCCACC AAAGACATGA GGAAATCACA GGAGAGATCG 
ATGTCTTATT CTGATGAGTC TCGACTGTCG AATCTTCTTC GGAGGATCAC CCGGGAAGAC 
GACAGAGACC GAAGATTGGC TACTGTAAAG CAGTTGAAAG AATTTATTCA GCAACCAGAA 
AATAAGCTGG TACTAGTTAA ACAATTGGAT AATATCTTGG CTGCTGTACA TGACGTGCTT 
AATGAAAGTA GCAAATTGCT TCAGGAGTTG AGACAGGAGG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTGAGCTA TGAGGCTGAG AAGATCTTCA AGTGGATTTT TAGCAAATTT 
AGCTCATCTG CAAAAGATGA AGTTAAACTC CTCTACTTAT GTGCCACCTA CAAAGCACTA 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTGTAATGC AGCTTGTAAT GACCAGCCTG 
CAGTCTATTC TTGAAAATGT GGATACACCA GAATTGCTTT GTAAATGTGT TAAGTGCATT 
CTTTTGGTGG CTCGATGTTA CCCTCATATT TTCAGCACTA ATTTTAGGGA TACAGTTGAT 
ATATTAGTTG GATGGCATAT AGATCATACT CAGAAACCTT CGCTCACGCA GCAGGTATCT 
GGGTGGTTGC AGAGTTTGGA GCCATTTTGG GTAGCTGATC TTGCATTTTC TACTACTCTT 
CTTGGTCAGT TTCTGGAAGA CATGGAAGCA TATGCTGAGG ACCTCAGCCA TGTGGCCTCT 
GGGGAATCAG TGGATGAAGA TGTCCCTCCT CCATCAGTGT CATTACCAAA GCTGGCTGCA 
CTTCTCCGGG TATTTAGTAC TGTGGTGAGG AGCATTGGGG AACGCTTCAG CCCAATTCGG 
GGTCCTCCAA TTACTGAGGC ATATGTAACA GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACGGCTGCAA ACCAGGTGTT TTTTTCTGAG GCTGTGTTGA CAGCTGCTAA TGAGTGTGTT 
GGTGTTTTGC TCGGCAGCTT GGATCCTAGC ATGACTATAC ATTGTGACAT GGTCATTACA 
TATGGATTAG ACCAACTGGA GAATTGCCAG ACTTGTGGTA CCGATTATAT CATCTCAGTC 
TTGAATTTAC TCACGCTGAT TGTTGAACAG ATAAATACGA AACTGCCATC ATCATTTGTA 
GAAAAACTGT TTATACCATC ATCTAAACTA CTATTCTTGC GTTATCATAA AGAAAAAGAG 
GTTGTTGCTG TAGCCCATGC TGTTTATCAA GCAGTGCTCA GCTTGAAGAA TATTCCTGTT 
TTGGAGACTG CCTATAAGTT AATATTGGGA GAAATGACTT GTGCCCTAAA CAACCTCCTA 
CACAGTCTAC AACTTCCTGA GGCCTGTTCT GAAATAAAAC ATGAGGCTTT TAAGAATCAT 
GTGTTCAATG TAGACAATGC AAAATTTGTA GTTATATTTG ACCTCAGTGC CCTGACTACA 
ATTGGAAATG CCAAAAACTC ACTAATAGGG ATGTGGGCGC TATCTCCAAC TGTCTTTGCA 
CTTCTGAGTA AGAATCTGAT GATTGTGCAC AGTGACCTGG CTGTTCACTT CCCTGCCATT 
CAGTATGCTG TGCTCTACAC ATTGTATTCT CATTGTACCA GGCATGATCA CTTTATCTCT 
AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG TTTGATGGAG CTGTGATTAG CACTGTAACT 
ACGGCTACAA AGAAACATTT CTCAATTATA TTAAATCTTC TGGGAATATT ACTTAAGAAA 
GATAACCTTA ACCAGGACAC GAGGAAACTG TTAATGACTT GGGCTTTGGA AGCAGCTGTT 
TTAATGAAGA AGTCTGAAAC ATACGCACCT TTATTCTCTC TTCCGTCTTT CCATAAATTT 
TGCAAAGGCC TTTTAGCCAA CACTCTCGTT GAAGATGTGA ATATCTGTCT GCAGGCATGC 
AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG CCAGATGATC TTTTACAGAG ATGTGTCGAT 
GTTTGCCGTG TTCAACTAGT GCACAGTGGA ACTCGTATTC GACAAGCATT TGGAAAACTG 
TTGAAATCAA TTCCTTTAGA TGTTGTCCTA AGCAATAACA ATCACACAGA AATTCAAGAA 
ATTTCTTTAG CATTAAGAAG TCACATGAGT AAAGCACCAA GTAATACATT CCACCCCCAA 
GATTTCTCTG ATGTTATTAG TTTTATTTTG TATGGGAACT CTCATAGAAC AGGGAAGGAC 
AATTGGTTGG AAAGACTGTT CTATAGCTGC CAGAGACTGG ATAAGCGTGA CCAGTCAACA 
ATTCCACGCA ATCTCCTGAA GACAGATGCT GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT 
GCACAATTCA CTGTTCTTTC TAAGCTGAGA ACCCCACTGG GCAGAGCTCA AGACACCTTC 
CAGACAATTG AAGGTATCAT TCGAAGTCTC GCAGCTCACA CATTAAACCC TGATCAGGAT 
GTTAGTCAGT GGACAACTGC AGACAATGAT GAAGGCCATG GTAACAACCA ACTTAGACTT 
GTTCTTCTTC TGCAGTATCT GGAAAATCTG GAGAAATTAA TGTATAATGC ATACGAGGGA 
TGTGCTAATG CATTAACTTC ACCTCCCAAG GTCATTAGAA CTTTTTTCTA TACCAATCGC 
CAAACTTGTC AGGACTGGCT AACGCGGATT CGACTCTCCA TCATGAGGGT AGGATTGTTG 
GCAGGCCAGC CTGCAGTGAC AGTGAGACAT GGCTTTGACT TGCTTACAGA GATGAAAACA 
ACCAGCCTAT CTCAGGGGAA TGAATTGGAA GTAACCATTA TGATGGTGGT AGAAGCATTA 
TGTGAACTTC ATTGTCCTGA AGCTATACAG GGAATTGCTG TCTGGTCATC ATCTATTGTT 
GGAAAAAATC TTCTGTGGAT TAACTCAGTG GCTCAACAGG CTGAAGGGAG GTTTGAAAAG 
GCCTCTGTGG AGTACCAGGA ACACCTGTGT GCCATGACAG GTGTTGATTG CTGCATCTCC 
AGCTTTGACA AATCGGTGCT CACCTTAGCC AATGCTGGGC GTAACAGTGC CAGCCCGAAA 
CATTCTCTGA ATGGTGAATC CAGAAAAACT GTGCTGTCCA AACCGACTGA CTCTTCCCCT 
GAGGTTATAA ATTATTTAGG AAATAAAGCA TGTGAGTGCT ACATCTCAAT TGCCGATTGG 
GCTGCTGTGC AGGAATGGCA GAACGCTATC CATGACTTGA AAAAGAGTAC CAGTAGCACT 
TCCCTCAACC TGAAAGCTGA CTTCAACTAT ATAAAATCAT TAAGCAGCTT TGAGTCTGGA 
AAATTTGTTG AATGTACCGA GCAGTTAGAA TTGTTACCAG GAGAAAATAT CAATCTACTT 
GCTGGAGGAT CAAAAGAAAA AATAGACATG AAAAAACTGC TTCCTAACAT GTTAAGTCCG 
GATCCGAGGG AACTTCAGAA ATCCATTGAA GTTCAATTGT TAAGAAGTTC TGTTTGTTTG 
GCAACTGCTT TAAACCCGAT AGAACAAGAT CAGAAGTGGC AGTCTATAAC TGAAAATGTG 
GTAAAGTACT TGAAGCAAAC ATCCCGCATC GCTATTGGAC CTCTGAGACT TTCTACTTTA 
ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT ACCTTGCAGC TGTATTGCTC ATCTGCTTTG 
GAGAACACAG TTTCTAACAG ACTTTCAACA GAGGACTGTC TTATTCCACT CTTCAGTGAA 
GCTTTACGTT CATGTAAACA GCATGACGTG AGGCCATGGA TGCAGGCATT AAGGTATACT 
ATGTACCAGA ATCAGTTGTT GGAGAAAATT AAAGAACAAA CAGTCCCAAT TAGAAGCCAT 
CTCATGGAAT TAGGTCTAAC AGCAGCAAAA TTTGCTAGAA AACGAGGGAA TGTGTCCCTT 
GCAACAAGAC TGCTGGCACA GTGCAGTGAA GTTCAGCTGG GAAAGACCAC CACTGCACAG 
GATTTAGTCC AACATTTTAA AAAACTATCA ACCCAAGGTC AAGTGGATGA AAAATGGGGG 
CCCGAACTTG ATATTGAAAA AACCAAATTG CTTTATACAG CAGGCCAGTC AACACATGCA 
ATGGAAATGT TGAGTTCTTG TGCCATATCT TTCTGCAAGT CTGTGAAAGC TGAATATGCA 
GTTGCTAAAT CAATTCTGAC ACTGGCTAAA TGGATCCAGG CAGAATGGAA AGAGATTTCA 
GGACAGCTGA AACAGGTTTA CAGAGCTCAG CACCAACAGA ACTTCACAGG TCTTTCTACT 
TTGTCTAAAA ACATACTCAC TCTAATAGAA CTGCCATCTG TTAATACGAT GGAAGAAGAG 
TATCCTCGGA TCGAGAGTGA ATCTACAGTG CATATTGGAG TTGGAGAACC TGACTTCATT 
TTGGGACAGT TGTATCACCT GTCTTCAGTA CAGGCACCTG AAGTAGCCAA ATCTTGGGCA 
GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC AGAAAGGTGG TTGACAATGC CAGTCAGGGA 
GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA TCTGAAGTTC AGAATCTACT TCCAGACACT 
ATAACTGAGG AAGAGAAAGA GAGAATATAT GGTATTCTTG GACAGGCTGT GTGTCGGCCG 
GCGGGGATTC AGGATGAAGA TATAACACTT CAGATAACTG AGAGTGAAGA CAACGAAGAA 
GATGACATGG TTGATGTTAT CTGGCGTCAG TTGATATCAA GCTGCCCATG GCTTTCAGAA 
CTTGATGAAA GTGCAACTGA AGGAGTTATT AAAGTGTGGA GGAAAGTTGT AGATAGAATA 
TTCAGCCTGT ACAAACTCTC TTGCAGTGCA TACTTTACTT TCCTTAAACT CAACGCTGGT 
CAAATTCCTT TAGATGAGGA TGACCCTAGG CTGCATTTAA GTCACAGAGT GGAACAGAGC 
ACTGATGACA TGATTGTGAT GGCCACATTG CGCCTGCTGC GGTTGCTCGT GAAGCATGCT 
GGTGAGCTTC GGCAGTATCT GGAGCACGGC TTGGAGACAA CACCCACTGC ACCATGGAGA 
GGAATTATTC CGCAACTTTT CTCACGCTTA AACCACCCTG AAGTGTATGT GCGCCAAAGT 
ATTTGTAACC TTCTCTGCCG TGTGGCTCAA GATTCCCCAC ATCTCATATT GTATCCTGCA 
ATAGTGGGTA CCATATCGCT TAGTAGTGAA TCCCAGGCTT CAGGAAATAA ATTTTCCACT 
GCAATTCCAA CTTTACTTGG CAATATTCAA GGAGAAGAAT TGCTGGTTTC TGAATGTGAG 
GGAGGAAGTC CTCCTGCATC TCAGGATAGC AATAAGGATG AACCTAAAAG TGGATTAAAT 
GAAGACCAAG CCATGATGCA GGATTGTTAC AGCAAAATTG TAGATAAGCT GTCCTCTGCA 
AACCCCACCA TGGTATTACA GGTTCAGATG CTCGTGGCTG AACTGCGCAG GGTCACTGTG 
CTCTGGGATG AGCTCTGGCT GGGAGTTTTG CTGCAACAAC ACATGTATGT CCTGAGACGA 
ATTCAGCAGC TTGAAGATGA GGTGAAGAGA GTCCAGAACA ACAACACCTT ACGCAAAGAA 
GAGAAAATTG CAATCATGAG GGAGAAGCAC ACAGCTTTGA TGAAGCCCAT CGTATTTGCT 
TTGGAGCATG TGAGGAGTAT CACAGCGGCT CCTGCAGAAA CACCTCATGA AAAATGGTTT 
CAGGATAACT ATGGTGATGC CATTGAAAAT GCCCTAGAAA AACTGAAGAC TCCATTGAAC 
CCTGCAAAGC CTGGGAGCAG CTGGATTCCA TTTAAAGAGA TAATGCTAAG TTTGCAACAG 
AGAGCACAGA AACGTGCAAG TTACATCTTG CGTCTTGAAG AAATCAGTCC ATGGTTGGCT 
GCCATGACTA ACACTGAAAT TGCTCTTCCT GGGGAAGTCT CAGCCAGAGA CACTGTCACA 
ATCCATAGTG TGGGCGGAAC CATCACAATC TTACCGACTA AAACCAAGCC AAAGAAACTT 
CTCTTTCTTG GATCAGATGG GAAGAGCTAT CCTTATCTTT TCAAAGGACT GGAGGATTTA 
CATCTGGATG AGAGAATAAT GCAGTTCCTA TCTATTGTGA ATACCATGTT TGCTACAATT 
AATCGCCAAG AAACACCCCG GTTCCATGCT CGACACTATT CTGTAACACC ACTAGGAACA 
AGATCAGGAC TAATCCAGTG GGTAGATGGA GCCACACCCT TATTTGGTCT TTACAAACGA 
TGGCAACAAC GGGAAGCTGC CTTACAAGCA CAAAAGGCCC AAGATTCCTA CCAAACTCCT 
CAGAATCCTG GAATTGTACC CCGTCCTAGT GAACTTTATT ACAGTAAAAT TGGCCCTGCT 
TTGAAAACAG TTGGGCTTAG CCTGGATGTG TCCCGTCGGG ATTGGCCTCT TCATGTAATG 
AAGGCAGTAT TGGAAGAGTT AATGGAGGCC ACACCCCCGA ATCTCCTTGC CAAAGAGCTC 
TGGTCATCTT GCACAACACC TGATGAATGG TGGAGAGTTA CGCAGTCTTA TGCAAGATCT 
ACTGCAGTCA TGTCTATGGT TGGATACATA ATTGGCCTTG GAGACAGACA TCTGGATAAT 
GTTCTTATAG ATATGACGAC TGGAGAAGTT GTTCACATAG ATTACAATGT TTGCTTTGAA 
AAAGGTAATA TAAAAGTAGA CTTTACATTT ATGAGGGCCA AATGTTTTTT GGTGAAAGGG 
CAAAAATATA ATGATTTGTC TTTTCAAGGT AAAAGCCTTA GAGTTCCTGA GAAAGTACCT 
TTTCGAATGA CACAAAACAT TGAAACAGCA CTGGGTGTAA CTGGAGTAGA AGGTGTATTT 
AGGCTTTCAT GTGAGCAGGT TTTACACATT ATGCGGCGTG GCAGAGAGAC CCTGCTGACG 
CTGCTGGAGG CCTTTGTGTA CGACCCTCTG GTGGACTGGA CAGCAGGAGG CGAGGCTGGG 
TTTGCTGGTG CTGTCTATGG TGGAGGTGGC CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA 
GAGATGGAGC GAGAGATCAC CCGCAGCCTG TTTTCTTCTA GAGTAGCTGA GATTAAGGTG 
AACTGGTTTA AGAATAGAGA TGAGATGCTG GTTGTGCTTC CCAAGTTGGA CGGTAGCTTA 
GATGAATACC TAAGCTTGCA AGAGCAACTG ACAGATGTGG AAAAACTGCA GGGCAAACTA 
CTGGAGGAAA TAGAGTTTCT AGAAGGAGCT GAAGGGGTGG ATCATCCTTC TCATACTCTG 
CAACACAGGT ATTCTGAGCA CACCCAACTA CAGACTCAGC AAAGAGCTGT TCAGGAAGCA 
ATCCAGGTGA AGCTGAATGA ATTTGAACAA TGGATAACAC ATTATCAGGC TGCATTCAAT 
AATTTAGAAG CAACACAGCT TGCAAGCTTG CTTCAAGAGA TAAGCACACA AATGGACCTT 
GGTCCTCCAA GTTACGTGCC AGCAACAGCC TTTCTGCAGA ATGCTGGTCA GGCCCACTTG 
ATTAGCCAGT GCGAGCAGCT GGAGGGGGAG GTTGGTGCTC TCCTGCAGCA GAGGCGCTCC 
GTGCTCCGTG GCTGTCTGGA GCAACTGCAT CACTATGCAA CCGTGGCCCT GCAGTATCCG 
AAGGCCATAT TTCAGAAACA TCGAATTGAA CAGTGGAAGA CCTGGATGGA AGAGCTCATC 
TGTAACACCA CAGTAGAGCG TTGTCAAGAG CTCTATAGGA AATATGAAAT GCAATATGCT 
CCCCAGCCAC CCCCAACAGT GTGTCAGTTC ATCACTGCCA CTGAAATGAC CCTGCAGCGA 
TACGCAGCAG ACATCAACAG CAGACTTATT AGACAAGTGG AACGCTTGAA ACAGGAAGCT 
GTCACTGTGC CAGTTTGTGA AGATCAGTTG AAAGAAATTG AACGTTGCAT TAAAGTTTTC 
CTTCATGAGA ATGGAGAAGA AGGATCTTTG AGTCTAGCAA GTGTTATTAT TTCTGCCCTT 
TGTACCCTTA CAAGGCGTAA CCTGATGATG GAAGGTGCAG CGTCAAGTGC TGGAGAACAG 
CTGGTTGATC TGACTTCTCG GGATGGAGCC TGGTTCTTGG AGGAACTCTG CAGTATGAGC 
GGAAACGTCA CCTGCTTGGT TCAGTTACTG AAGCAGTGCC ACCTGGTGCC ACAGGACTTA 
GATATCCCGA ACCCCATGGA AGCGTCTGAG ACAGTTCACT TAGCCAATGG AGTGTATACC 
TCACTTCAGG AATTGAATTC GAATTTCCGG CAAATCATAT TTCCAGAAGC ACTTCGATGT 
TTAATGAAAG GGGAATACAC GTTAGAAAGT ATGCTGCATG AACTGGACGG TCTTATTGAG 
CAGACCACCG ATGGCGTTCC CCTGCAGACT CTAGTGGAAT CTCTTCAGGC CTACTTAAGA 
AACGCAGCTA TGGGACTGGA AGAAGAAACA CATGCTCATT ACATCGATGT TGCCAGACTA 
CTACATGCTC AGTACGGTGA ATTAATCCAA CCGAGAAATG GTTCAGTTGA TGAAACACCC 
AAAATGTCAG CTGGCCAGAT GCTTTTGGTA GCATTCGATG GCATGTTTGC TCAAGTTGAA 
ACTGCTTTCA GCTTATTAGT TGAAAAGTTG AACAAGATGG AAATTCCCAT AGCTTGGCGA 
AAGATTGACA TCATAAGGGA AGCCAGGAGT ACTCAAGTTA ATTTTTTTGA TGATGATAAT 
CACCGGCAGG TGCTAGAAGA GATTTTCTTT CTAAAAAGAC TACAGACTAT TAAGGAGTTC 
TTCAGGCTCT GTGGTACCTT TTCTAAAACA TTGTCAGGAT CAAGTTCACT TGAAGATCAG 
AATACTGTGA ATGGGCCTGT ACAGATTGTC AATGTGAAAA CCCTTTTTAG AAACTCTTGT 
TTCAGTGAAG ACCAAATGGC CAAACCTATC AAGGCATTCA CAGCTGACTT TGTGAGGCAG 
CTCTTGATAG GGCTACCCAA CCAAGCCCTC GGACTCACAC TGTGCAGTTT TATCAGTGCT 
CTGGGTGTAG ACATCATTGC TCAAGTAGAG GCAAAGGACT TTGGTGCCGA AAGCAAAGTT 
TCTGTTGATG ATCTCTGTAA GAAAGCGGTG GAACATAACA TCCAGATAGG GAAGTTCTCT 
CAGCTGGTTA TGAACAGGGC AACTGTGTTA GCAAGTTCTT ACGACACTGC CTGGAAGAAG 
CATGACTTGG TGCGAAGGCT AGAAACCAGT ATTTCTTCTT GTAAGACAAG CCTGCAGCGG 
GTTCAGCTGC ATATTGCCAT GTTTCAGTGG CAACATGAAG ATCTACTTAT CAATAGACCA 
CAAGCCATGT CAGTCACACC TCCCCCACGG TCTGCTATCC TAACCAGCAT GAAAAAGAAG 
CTGCATACCC TGAGCCAGAT TGAAACTTCT ATTGCAACAG TTCAGGAGAA GCTAGCTGCA 
CTTGAATCAA GTATTGAACA GCGACTCAAG TGGGCAGGTG GTGCCAACCC TGCATTGGCC 
CCTGTACTAC AAGATTTTGA AGCAACGATA GCTGAAAGAA GAAATCTTGT CCTTAAAGAG 
AGCCAAAGAG CAAGTCAGGT CACATTTCTC TGCAGCAATA TCATTCATTT TGAAAGTTTA 
CGAACAAGAA CTGCAGAAGC CTTAAACCTG GATGCGGCGT TATTTGAACT AATCAAGCGA 
TGTCAGCAGA TGTGTTCGTT TGCATCACAG TTTAACAGTT CAGTGTCTGA GTTAGAGCTT 
CGTTTATTAC AGAGAGTGGA CACTGGTCTT GAACATCCTA TTGGCAGCTC TGAATGGCTT 
TTGTCAGCAC ACAAACAGTT GACCCAGGAT ATGTCTACTC AGAGGGCAAT TCAGACAGAG 
AAAGAGCAGC AGATAGAAAC GGTCTGTGAA ACAATTCAGA ATCTGGTTGA TAATATAAAG 
ACTGTGCTCA CTGGTCATAA CCGACAGCTT GGAGATGTCA AACATCTCTT GAAAGCTATG 
GCTAAGGATG AAGAAGCTGC TCTGGCAGAT GGTGAAGATG TTCCCTATGA GAACAGTGTT 
AGGCAGTTTT TGGGTGAATA TAAATCATGG CAAGACAACA TTCAAACAGT TCTATTTACA 
TTAGTCCAGG CTATGGGTCA GGTTCGAAGT CAAGAACACG TTGAAATGCT CCAGGAAATC 
ACTCCCACCT TGAAAGAACT GAAAACACAA AGTCAGAGTA TCTATAATAA TTTAGTGAGT 
TTTGCATCAC CCTTAGTCAC CGATGCAACA AATGAATGTT CGAGTCCAAC GTCATCTGCT 
ACTTATCAGC CATCCTTCGC TGCAGTCCGG AGTAACACTG GCCAGAAGAC TCAGCCTGAT 
GTCATGTCAC AGAATGCTAG AAAGCTGATC CAAAAAAATC TTGCTACATC AGCTGATACT 
CCACCAAGCA CCGTTCCAGG AACTGGCAAG AGTGTTGCTT GTAGTCCTAA AAAGGCAGTC 
AGAGACCCTA AAACTGGGAA AGCGGTGCAA GAGAGAAACT CCTATGCAGT GAGTGTGTGG 
AAGAGAGTGA AAGCCAAGTT AGAGGGCCGA GATGTTGATC CGAATAGGAG GATGTCAGTT 
GCTGAACAGG TTGACTATGT CATTAAGGAA GCAACTAATC TAGATAACTT GGCTCAGCTG 
TATGAAGGTT GGACAGCCTG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544071.1
CDS	378..11444
Translation	MSRRAPGSRLSSGGGGGGTKYPRSWNDWQPRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSVQHGSRALATKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGNIKVDFTFMRAKCFLVKGQKYNDLSFQGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_011545769.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545769.3

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGGGTT AACAATGTGA CCAGCCCTGA GTTCACCAGT 
GTTCAGCATG GCAGTCGTGC TTTAGCCACC AAAGACATGA GGAAATCACA GGAGAGATCG 
ATGTCTTATT CTGATGAGTC TCGACTGTCG AATCTTCTTC GGAGGATCAC CCGGGAAGAC 
GACAGAGACC GAAGATTGGC TACTGTAAAG CAGTTGAAAG AATTTATTCA GCAACCAGAA 
AATAAGCTGG TACTAGTTAA ACAATTGGAT AATATCTTGG CTGCTGTACA TGACGTGCTT 
AATGAAAGTA GCAAATTGCT TCAGGAGTTG AGACAGGAGG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTGAGCTA TGAGGCTGAG AAGATCTTCA AGTGGATTTT TAGCAAATTT 
AGCTCATCTG CAAAAGATGA AGTTAAACTC CTCTACTTAT GTGCCACCTA CAAAGCACTA 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTGTAATGC AGCTTGTAAT GACCAGCCTG 
CAGTCTATTC TTGAAAATGT GGATACACCA GAATTGCTTT GTAAATGTGT TAAGTGCATT 
CTTTTGGTGG CTCGATGTTA CCCTCATATT TTCAGCACTA ATTTTAGGGA TACAGTTGAT 
ATATTAGTTG GATGGCATAT AGATCATACT CAGAAACCTT CGCTCACGCA GCAGGTATCT 
GGGTGGTTGC AGAGTTTGGA GCCATTTTGG GTAGCTGATC TTGCATTTTC TACTACTCTT 
CTTGGTCAGT TTCTGGAAGA CATGGAAGCA TATGCTGAGG ACCTCAGCCA TGTGGCCTCT 
GGGGAATCAG TGGATGAAGA TGTCCCTCCT CCATCAGTGT CATTACCAAA GCTGGCTGCA 
CTTCTCCGGG TATTTAGTAC TGTGGTGAGG AGCATTGGGG AACGCTTCAG CCCAATTCGG 
GGTCCTCCAA TTACTGAGGC ATATGTAACA GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACGGCTGCAA ACCAGGTGTT TTTTTCTGAG GCTGTGTTGA CAGCTGCTAA TGAGTGTGTT 
GGTGTTTTGC TCGGCAGCTT GGATCCTAGC ATGACTATAC ATTGTGACAT GGTCATTACA 
TATGGATTAG ACCAACTGGA GAATTGCCAG ACTTGTGGTA CCGATTATAT CATCTCAGTC 
TTGAATTTAC TCACGCTGAT TGTTGAACAG ATAAATACGA AACTGCCATC ATCATTTGTA 
GAAAAACTGT TTATACCATC ATCTAAACTA CTATTCTTGC GTTATCATAA AGAAAAAGAG 
GTTGTTGCTG TAGCCCATGC TGTTTATCAA GCAGTGCTCA GCTTGAAGAA TATTCCTGTT 
TTGGAGACTG CCTATAAGTT AATATTGGGA GAAATGACTT GTGCCCTAAA CAACCTCCTA 
CACAGTCTAC AACTTCCTGA GGCCTGTTCT GAAATAAAAC ATGAGGCTTT TAAGAATCAT 
GTGTTCAATG TAGACAATGC AAAATTTGTA GTTATATTTG ACCTCAGTGC CCTGACTACA 
ATTGGAAATG CCAAAAACTC ACTAATAGGG ATGTGGGCGC TATCTCCAAC TGTCTTTGCA 
CTTCTGAGTA AGAATCTGAT GATTGTGCAC AGTGACCTGG CTGTTCACTT CCCTGCCATT 
CAGTATGCTG TGCTCTACAC ATTGTATTCT CATTGTACCA GGCATGATCA CTTTATCTCT 
AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG TTTGATGGAG CTGTGATTAG CACTGTAACT 
ACGGCTACAA AGAAACATTT CTCAATTATA TTAAATCTTC TGGGAATATT ACTTAAGAAA 
GATAACCTTA ACCAGGACAC GAGGAAACTG TTAATGACTT GGGCTTTGGA AGCAGCTGTT 
TTAATGAAGA AGTCTGAAAC ATACGCACCT TTATTCTCTC TTCCGTCTTT CCATAAATTT 
TGCAAAGGCC TTTTAGCCAA CACTCTCGTT GAAGATGTGA ATATCTGTCT GCAGGCATGC 
AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG CCAGATGATC TTTTACAGAG ATGTGTCGAT 
GTTTGCCGTG TTCAACTAGT GCACAGTGGA ACTCGTATTC GACAAGCATT TGGAAAACTG 
TTGAAATCAA TTCCTTTAGA TGTTGTCCTA AGCAATAACA ATCACACAGA AATTCAAGAA 
ATTTCTTTAG CATTAAGAAG TCACATGAGT AAAGCACCAA GTAATACATT CCACCCCCAA 
GATTTCTCTG ATGTTATTAG TTTTATTTTG TATGGGAACT CTCATAGAAC AGGGAAGGAC 
AATTGGTTGG AAAGACTGTT CTATAGCTGC CAGAGACTGG ATAAGCGTGA CCAGTCAACA 
ATTCCACGCA ATCTCCTGAA GACAGATGCT GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT 
GCACAATTCA CTGTTCTTTC TAAGCTGAGA ACCCCACTGG GCAGAGCTCA AGACACCTTC 
CAGACAATTG AAGGTATCAT TCGAAGTCTC GCAGCTCACA CATTAAACCC TGATCAGGAT 
GTTAGTCAGT GGACAACTGC AGACAATGAT GAAGGCCATG GTAACAACCA ACTTAGACTT 
GTTCTTCTTC TGCAGTATCT GGAAAATCTG GAGAAATTAA TGTATAATGC ATACGAGGGA 
TGTGCTAATG CATTAACTTC ACCTCCCAAG GTCATTAGAA CTTTTTTCTA TACCAATCGC 
CAAACTTGTC AGGACTGGCT AACGCGGATT CGACTCTCCA TCATGAGGGT AGGATTGTTG 
GCAGGCCAGC CTGCAGTGAC AGTGAGACAT GGCTTTGACT TGCTTACAGA GATGAAAACA 
ACCAGCCTAT CTCAGGGGAA TGAATTGGAA GTAACCATTA TGATGGTGGT AGAAGCATTA 
TGTGAACTTC ATTGTCCTGA AGCTATACAG GGAATTGCTG TCTGGTCATC ATCTATTGTT 
GGAAAAAATC TTCTGTGGAT TAACTCAGTG GCTCAACAGG CTGAAGGGAG GTTTGAAAAG 
GCCTCTGTGG AGTACCAGGA ACACCTGTGT GCCATGACAG GTGTTGATTG CTGCATCTCC 
AGCTTTGACA AATCGGTGCT CACCTTAGCC AATGCTGGGC GTAACAGTGC CAGCCCGAAA 
CATTCTCTGA ATGGTGAATC CAGAAAAACT GTGCTGTCCA AACCGACTGA CTCTTCCCCT 
GAGGTTATAA ATTATTTAGG AAATAAAGCA TGTGAGTGCT ACATCTCAAT TGCCGATTGG 
GCTGCTGTGC AGGAATGGCA GAACGCTATC CATGACTTGA AAAAGAGTAC CAGTAGCACT 
TCCCTCAACC TGAAAGCTGA CTTCAACTAT ATAAAATCAT TAAGCAGCTT TGAGTCTGGA 
AAATTTGTTG AATGTACCGA GCAGTTAGAA TTGTTACCAG GAGAAAATAT CAATCTACTT 
GCTGGAGGAT CAAAAGAAAA AATAGACATG AAAAAACTGC TTCCTAACAT GTTAAGTCCG 
GATCCGAGGG AACTTCAGAA ATCCATTGAA GTTCAATTGT TAAGAAGTTC TGTTTGTTTG 
GCAACTGCTT TAAACCCGAT AGAACAAGAT CAGAAGTGGC AGTCTATAAC TGAAAATGTG 
GTAAAGTACT TGAAGCAAAC ATCCCGCATC GCTATTGGAC CTCTGAGACT TTCTACTTTA 
ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT ACCTTGCAGC TGTATTGCTC ATCTGCTTTG 
GAGAACACAG TTTCTAACAG ACTTTCAACA GAGGACTGTC TTATTCCACT CTTCAGTGAA 
GCTTTACGTT CATGTAAACA GCATGACGTG AGGCCATGGA TGCAGGCATT AAGGTATACT 
ATGTACCAGA ATCAGTTGTT GGAGAAAATT AAAGAACAAA CAGTCCCAAT TAGAAGCCAT 
CTCATGGAAT TAGGTCTAAC AGCAGCAAAA TTTGCTAGAA AACGAGGGAA TGTGTCCCTT 
GCAACAAGAC TGCTGGCACA GTGCAGTGAA GTTCAGCTGG GAAAGACCAC CACTGCACAG 
GATTTAGTCC AACATTTTAA AAAACTATCA ACCCAAGGTC AAGTGGATGA AAAATGGGGG 
CCCGAACTTG ATATTGAAAA AACCAAATTG CTTTATACAG CAGGCCAGTC AACACATGCA 
ATGGAAATGT TGAGTTCTTG TGCCATATCT TTCTGCAAGT CTGTGAAAGC TGAATATGCA 
GTTGCTAAAT CAATTCTGAC ACTGGCTAAA TGGATCCAGG CAGAATGGAA AGAGATTTCA 
GGACAGCTGA AACAGGTTTA CAGAGCTCAG CACCAACAGA ACTTCACAGG TCTTTCTACT 
TTGTCTAAAA ACATACTCAC TCTAATAGAA CTGCCATCTG TTAATACGAT GGAAGAAGAG 
TATCCTCGGA TCGAGAGTGA ATCTACAGTG CATATTGGAG TTGGAGAACC TGACTTCATT 
TTGGGACAGT TGTATCACCT GTCTTCAGTA CAGGCACCTG AAGTAGCCAA ATCTTGGGCA 
GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC AGAAAGGTGG TTGACAATGC CAGTCAGGGA 
GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA TCTGAAGTTC AGAATCTACT TCCAGACACT 
ATAACTGAGG AAGAGAAAGA GAGAATATAT GGTATTCTTG GACAGGCTGT GTGTCGGCCG 
GCGGGGATTC AGGATGAAGA TATAACACTT CAGATAACTG AGAGTGAAGA CAACGAAGAA 
GATGACATGG TTGATGTTAT CTGGCGTCAG TTGATATCAA GCTGCCCATG GCTTTCAGAA 
CTTGATGAAA GTGCAACTGA AGGAGTTATT AAAGTGTGGA GGAAAGTTGT AGATAGAATA 
TTCAGCCTGT ACAAACTCTC TTGCAGTGCA TACTTTACTT TCCTTAAACT CAACGCTGGT 
CAAATTCCTT TAGATGAGGA TGACCCTAGG CTGCATTTAA GTCACAGAGT GGAACAGAGC 
ACTGATGACA TGATTGTGAT GGCCACATTG CGCCTGCTGC GGTTGCTCGT GAAGCATGCT 
GGTGAGCTTC GGCAGTATCT GGAGCACGGC TTGGAGACAA CACCCACTGC ACCATGGAGA 
GGAATTATTC CGCAACTTTT CTCACGCTTA AACCACCCTG AAGTGTATGT GCGCCAAAGT 
ATTTGTAACC TTCTCTGCCG TGTGGCTCAA GATTCCCCAC ATCTCATATT GTATCCTGCA 
ATAGTGGGTA CCATATCGCT TAGTAGTGAA TCCCAGGCTT CAGGAAATAA ATTTTCCACT 
GCAATTCCAA CTTTACTTGG CAATATTCAA GGAGAAGAAT TGCTGGTTTC TGAATGTGAG 
GGAGGAAGTC CTCCTGCATC TCAGGATAGC AATAAGGATG AACCTAAAAG TGGATTAAAT 
GAAGACCAAG CCATGATGCA GGATTGTTAC AGCAAAATTG TAGATAAGCT GTCCTCTGCA 
AACCCCACCA TGGTATTACA GGTTCAGATG CTCGTGGCTG AACTGCGCAG GGTCACTGTG 
CTCTGGGATG AGCTCTGGCT GGGAGTTTTG CTGCAACAAC ACATGTATGT CCTGAGACGA 
ATTCAGCAGC TTGAAGATGA GGTGAAGAGA GTCCAGAACA ACAACACCTT ACGCAAAGAA 
GAGAAAATTG CAATCATGAG GGAGAAGCAC ACAGCTTTGA TGAAGCCCAT CGTATTTGCT 
TTGGAGCATG TGAGGAGTAT CACAGCGGCT CCTGCAGAAA CACCTCATGA AAAATGGTTT 
CAGGATAACT ATGGTGATGC CATTGAAAAT GCCCTAGAAA AACTGAAGAC TCCATTGAAC 
CCTGCAAAGC CTGGGAGCAG CTGGATTCCA TTTAAAGAGA TAATGCTAAG TTTGCAACAG 
AGAGCACAGA AACGTGCAAG TTACATCTTG CGTCTTGAAG AAATCAGTCC ATGGTTGGCT 
GCCATGACTA ACACTGAAAT TGCTCTTCCT GGGGAAGTCT CAGCCAGAGA CACTGTCACA 
ATCCATAGTG TGGGCGGAAC CATCACAATC TTACCGACTA AAACCAAGCC AAAGAAACTT 
CTCTTTCTTG GATCAGATGG GAAGAGCTAT CCTTATCTTT TCAAAGGACT GGAGGATTTA 
CATCTGGATG AGAGAATAAT GCAGTTCCTA TCTATTGTGA ATACCATGTT TGCTACAATT 
AATCGCCAAG AAACACCCCG GTTCCATGCT CGACACTATT CTGTAACACC ACTAGGAACA 
AGATCAGGAC TAATCCAGTG GGTAGATGGA GCCACACCCT TATTTGGTCT TTACAAACGA 
TGGCAACAAC GGGAAGCTGC CTTACAAGCA CAAAAGGCCC AAGATTCCTA CCAAACTCCT 
CAGAATCCTG GAATTGTACC CCGTCCTAGT GAACTTTATT ACAGTAAAAT TGGCCCTGCT 
TTGAAAACAG TTGGGCTTAG CCTGGATGTG TCCCGTCGGG ATTGGCCTCT TCATGTAATG 
AAGGCAGTAT TGGAAGAGTT AATGGAGGCC ACACCCCCGA ATCTCCTTGC CAAAGAGCTC 
TGGTCATCTT GCACAACACC TGATGAATGG TGGAGAGTTA CGCAGTCTTA TGCAAGATCT 
ACTGCAGTCA TGTCTATGGT TGGATACATA ATTGGCCTTG GAGACAGACA TCTGGATAAT 
GTTCTTATAG ATATGACGAC TGGAGAAGTT GTTCACATAG ATTACAATGT TTGCTTTGAA 
AAAGGTAATA TAAAAGTAGA CTTTACATTT ATGAGGGCCA AATGTTTTTT GGTGAAAGGG 
CAAAAATATA ATGATTTGTC TTTTCAAGGT AAAAGCCTTA GAGTTCCTGA GAAAGTACCT 
TTTCGAATGA CACAAAACAT TGAAACAGCA CTGGGTGTAA CTGGAGTAGA AGGTGTATTT 
AGGCTTTCAT GTGAGCAGGT TTTACACATT ATGCGGCGTG GCAGAGAGAC CCTGCTGACG 
CTGCTGGAGG CCTTTGTGTA CGACCCTCTG GTGGACTGGA CAGCAGGAGG CGAGGCTGGG 
TTTGCTGGTG CTGTCTATGG TGGAGGTGGC CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA 
GAGATGGAGC GAGAGATCAC CCGCAGCCTG TTTTCTTCTA GAGTAGCTGA GATTAAGGTG 
AACTGGTTTA AGAATAGAGA TGAGATGCTG GTTGTGCTTC CCAAGTTGGA CGGTAGCTTA 
GATGAATACC TAAGCTTGCA AGAGCAACTG ACAGATGTGG AAAAACTGCA GGGCAAACTA 
CTGGAGGAAA TAGAGTTTCT AGAAGGAGCT GAAGGGGTGG ATCATCCTTC TCATACTCTG 
CAACACAGGT ATTCTGAGCA CACCCAACTA CAGACTCAGC AAAGAGCTGT TCAGGAAGCA 
ATCCAGGTGA AGCTGAATGA ATTTGAACAA TGGATAACAC ATTATCAGGC TGCATTCAAT 
AATTTAGAAG CAACACAGCT TGCAAGCTTG CTTCAAGAGA TAAGCACACA AATGGACCTT 
GGTCCTCCAA GTTACGTGCC AGCAACAGCC TTTCTGCAGA ATGCTGGTCA GGCCCACTTG 
ATTAGCCAGT GCGAGCAGCT GGAGGGGGAG GTTGGTGCTC TCCTGCAGCA GAGGCGCTCC 
GTGCTCCGTG GCTGTCTGGA GCAACTGCAT CACTATGCAA CCGTGGCCCT GCAGTATCCG 
AAGGCCATAT TTCAGAAACA TCGAATTGAA CAGTGGAAGA CCTGGATGGA AGAGCTCATC 
TGTAACACCA CAGTAGAGCG TTGTCAAGAG CTCTATAGGA AATATGAAAT GCAATATGCT 
CCCCAGCCAC CCCCAACAGT GTGTCAGTTC ATCACTGCCA CTGAAATGAC CCTGCAGCGA 
TACGCAGCAG ACATCAACAG CAGACTTATT AGACAAGTGG AACGCTTGAA ACAGGAAGCT 
GTCACTGTGC CAGTTTGTGA AGATCAGTTG AAAGAAATTG AACGTTGCAT TAAAGTTTTC 
CTTCATGAGA ATGGAGAAGA AGGATCTTTG AGTCTAGCAA GTGTTATTAT TTCTGCCCTT 
TGTACCCTTA CAAGGCGTAA CCTGATGATG GAAGGTGCAG CGTCAAGTGC TGGAGAACAG 
CTGGTTGATC TGACTTCTCG GGATGGAGCC TGGTTCTTGG AGGAACTCTG CAGTATGAGC 
GGAAACGTCA CCTGCTTGGT TCAGTTACTG AAGCAGTGCC ACCTGGTGCC ACAGGACTTA 
GATATCCCGA ACCCCATGGA AGCGTCTGAG ACAGTTCACT TAGCCAATGG AGTGTATACC 
TCACTTCAGG AATTGAATTC GAATTTCCGG CAAATCATAT TTCCAGAAGC ACTTCGATGT 
TTAATGAAAG GGGAATACAC GTTAGAAAGT ATGCTGCATG AACTGGACGG TCTTATTGAG 
CAGACCACCG ATGGCGTTCC CCTGCAGACT CTAGTGGAAT CTCTTCAGGC CTACTTAAGA 
AACGCAGCTA TGGGACTGGA AGAAGAAACA CATGCTCATT ACATCGATGT TGCCAGACTA 
CTACATGCTC AGTACGGTGA ATTAATCCAA CCGAGAAATG GTTCAGTTGA TGAAACACCC 
AAAATGTCAG CTGGCCAGAT GCTTTTGGTA GCATTCGATG GCATGTTTGC TCAAGTTGAA 
ACTGCTTTCA GCTTATTAGT TGAAAAGTTG AACAAGATGG AAATTCCCAT AGCTTGGCGA 
AAGATTGACA TCATAAGGGA AGCCAGGAGT ACTCAAGTTA ATTTTTTTGA TGATGATAAT 
CACCGGCAGG TGCTAGAAGA GATTTTCTTT CTAAAAAGAC TACAGACTAT TAAGGAGTTC 
TTCAGGCTCT GTGGTACCTT TTCTAAAACA TTGTCAGGAT CAAGTTCACT TGAAGATCAG 
AATACTGTGA ATGGGCCTGT ACAGATTGTC AATGTGAAAA CCCTTTTTAG AAACTCTTGT 
TTCAGTGAAG ACCAAATGGC CAAACCTATC AAGGCATTCA CAGCTGACTT TGTGAGGCAG 
CTCTTGATAG GGCTACCCAA CCAAGCCCTC GGACTCACAC TGTGCAGTTT TATCAGTGCT 
CTGGGTGTAG ACATCATTGC TCAAGTAGAG GCAAAGGACT TTGGTGCCGA AAGCAAAGTT 
TCTGTTGATG ATCTCTGTAA GAAAGCGGTG GAACATAACA TCCAGATAGG GAAGTTCTCT 
CAGCTGGTTA TGAACAGGGC AACTGTGTTA GCAAGTTCTT ACGACACTGC CTGGAAGAAG 
CATGACTTGG TGCGAAGGCT AGAAACCAGT ATTTCTTCTT GTAAGACAAG CCTGCAGCGG 
GTTCAGCTGC ATATTGCCAT GTTTCAGTGG CAACATGAAG ATCTACTTAT CAATAGACCA 
CAAGCCATGT CAGTCACACC TCCCCCACGG TCTGCTATCC TAACCAGCAT GAAAAAGAAG 
CTGCATACCC TGAGCCAGAT TGAAACTTCT ATTGCAACAG TTCAGGAGAA GCTAGCTGCA 
CTTGAATCAA GTATTGAACA GCGACTCAAG TGGGCAGGTG GTGCCAACCC TGCATTGGCC 
CCTGTACTAC AAGATTTTGA AGCAACGATA GCTGAAAGAA GAAATCTTGT CCTTAAAGAG 
AGCCAAAGAG CAAGTCAGGT CACATTTCTC TGCAGCAATA TCATTCATTT TGAAAGTTTA 
CGAACAAGAA CTGCAGAAGC CTTAAACCTG GATGCGGCGT TATTTGAACT AATCAAGCGA 
TGTCAGCAGA TGTGTTCGTT TGCATCACAG TTTAACAGTT CAGTGTCTGA GTTAGAGCTT 
CGTTTATTAC AGAGAGTGGA CACTGGTCTT GAACATCCTA TTGGCAGCTC TGAATGGCTT 
TTGTCAGCAC ACAAACAGTT GACCCAGGAT ATGTCTACTC AGAGGGCAAT TCAGACAGAG 
AAAGAGCAGC AGATAGAAAC GGTCTGTGAA ACAATTCAGA ATCTGGTTGA TAATATAAAG 
ACTGTGCTCA CTGGTCATAA CCGACAGCTT GGAGATGTCA AACATCTCTT GAAAGCTATG 
GCTAAGGATG AAGAAGCTGC TCTGGCAGAT GGTGAAGATG TTCCCTATGA GAACAGTGTT 
AGGCAGTTTT TGGGTGAATA TAAATCATGG CAAGACAACA TTCAAACAGT TCTATTTACA 
TTAGTCCAGG CTATGGGTCA GGTTCGAAGT CAAGAACACG TTGAAATGCT CCAGGAAATC 
ACTCCCACCT TGAAAGAACT GAAAACACAA AGTCAGAGTA TCTATAATAA TTTAGTGAGT 
TTTGCATCAC CCTTAGTCAC CGATGCAACA AATGAATGTT CGAGTCCAAC GTCATCTGCT 
ACTTATCAGC CATCCTTCGC TGCAGTCCGG AGTAACACTG GCCAGAAGAC TCAGCCTGAT 
GTCATGTCAC AGAATGCTAG AAAGCTGATC CAAAAAAATC TTGCTACATC AGCTGATACT 
CCACCAAGCA CCGTTCCAGG AACTGGCAAG AGTGTTGCTT GTAGTCCTAA AAAGGCAGTC 
AGAGACCCTA AAACTGGGAA AGCGGTGCAA GAGAGAAACT CCTATGCAGT GAGTGTGTGG 
AAGAGAGTGA AAGCCAAGTT AGAGGGCCGA GATGTTGATC CGAATAGGAG GATGTCAGTT 
GCTGAACAGG TTGACTATGT CATTAAGGAA GCAACTAATC TAGATAACTT GGCTCAGCTG 
TATGAAGGTT GGACAGCCTG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_011545769.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544071.1 Homo sapiens SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

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CloneID	OHu65357
Clone ID Related Accession (Same CDS sequence)	XM_011545769.3 , XM_011545769.2
Accession Version	XM_011545769.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGGGTT AACAATGTGA CCAGCCCTGA GTTCACCAGT 
GTTCAGCATG GCAGTCGTGC TTTAGCCACC AAAGACATGA GGAAATCACA GGAGAGATCG 
ATGTCTTATT CTGATGAGTC TCGACTGTCG AATCTTCTTC GGAGGATCAC CCGGGAAGAC 
GACAGAGACC GAAGATTGGC TACTGTAAAG CAGTTGAAAG AATTTATTCA GCAACCAGAA 
AATAAGCTGG TACTAGTTAA ACAATTGGAT AATATCTTGG CTGCTGTACA TGACGTGCTT 
AATGAAAGTA GCAAATTGCT TCAGGAGTTG AGACAGGAGG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTGAGCTA TGAGGCTGAG AAGATCTTCA AGTGGATTTT TAGCAAATTT 
AGCTCATCTG CAAAAGATGA AGTTAAACTC CTCTACTTAT GTGCCACCTA CAAAGCACTA 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTGTAATGC AGCTTGTAAT GACCAGCCTG 
CAGTCTATTC TTGAAAATGT GGATACACCA GAATTGCTTT GTAAATGTGT TAAGTGCATT 
CTTTTGGTGG CTCGATGTTA CCCTCATATT TTCAGCACTA ATTTTAGGGA TACAGTTGAT 
ATATTAGTTG GATGGCATAT AGATCATACT CAGAAACCTT CGCTCACGCA GCAGGTATCT 
GGGTGGTTGC AGAGTTTGGA GCCATTTTGG GTAGCTGATC TTGCATTTTC TACTACTCTT 
CTTGGTCAGT TTCTGGAAGA CATGGAAGCA TATGCTGAGG ACCTCAGCCA TGTGGCCTCT 
GGGGAATCAG TGGATGAAGA TGTCCCTCCT CCATCAGTGT CATTACCAAA GCTGGCTGCA 
CTTCTCCGGG TATTTAGTAC TGTGGTGAGG AGCATTGGGG AACGCTTCAG CCCAATTCGG 
GGTCCTCCAA TTACTGAGGC ATATGTAACA GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACGGCTGCAA ACCAGGTGTT TTTTTCTGAG GCTGTGTTGA CAGCTGCTAA TGAGTGTGTT 
GGTGTTTTGC TCGGCAGCTT GGATCCTAGC ATGACTATAC ATTGTGACAT GGTCATTACA 
TATGGATTAG ACCAACTGGA GAATTGCCAG ACTTGTGGTA CCGATTATAT CATCTCAGTC 
TTGAATTTAC TCACGCTGAT TGTTGAACAG ATAAATACGA AACTGCCATC ATCATTTGTA 
GAAAAACTGT TTATACCATC ATCTAAACTA CTATTCTTGC GTTATCATAA AGAAAAAGAG 
GTTGTTGCTG TAGCCCATGC TGTTTATCAA GCAGTGCTCA GCTTGAAGAA TATTCCTGTT 
TTGGAGACTG CCTATAAGTT AATATTGGGA GAAATGACTT GTGCCCTAAA CAACCTCCTA 
CACAGTCTAC AACTTCCTGA GGCCTGTTCT GAAATAAAAC ATGAGGCTTT TAAGAATCAT 
GTGTTCAATG TAGACAATGC AAAATTTGTA GTTATATTTG ACCTCAGTGC CCTGACTACA 
ATTGGAAATG CCAAAAACTC ACTAATAGGG ATGTGGGCGC TATCTCCAAC TGTCTTTGCA 
CTTCTGAGTA AGAATCTGAT GATTGTGCAC AGTGACCTGG CTGTTCACTT CCCTGCCATT 
CAGTATGCTG TGCTCTACAC ATTGTATTCT CATTGTACCA GGCATGATCA CTTTATCTCT 
AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG TTTGATGGAG CTGTGATTAG CACTGTAACT 
ACGGCTACAA AGAAACATTT CTCAATTATA TTAAATCTTC TGGGAATATT ACTTAAGAAA 
GATAACCTTA ACCAGGACAC GAGGAAACTG TTAATGACTT GGGCTTTGGA AGCAGCTGTT 
TTAATGAAGA AGTCTGAAAC ATACGCACCT TTATTCTCTC TTCCGTCTTT CCATAAATTT 
TGCAAAGGCC TTTTAGCCAA CACTCTCGTT GAAGATGTGA ATATCTGTCT GCAGGCATGC 
AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG CCAGATGATC TTTTACAGAG ATGTGTCGAT 
GTTTGCCGTG TTCAACTAGT GCACAGTGGA ACTCGTATTC GACAAGCATT TGGAAAACTG 
TTGAAATCAA TTCCTTTAGA TGTTGTCCTA AGCAATAACA ATCACACAGA AATTCAAGAA 
ATTTCTTTAG CATTAAGAAG TCACATGAGT AAAGCACCAA GTAATACATT CCACCCCCAA 
GATTTCTCTG ATGTTATTAG TTTTATTTTG TATGGGAACT CTCATAGAAC AGGGAAGGAC 
AATTGGTTGG AAAGACTGTT CTATAGCTGC CAGAGACTGG ATAAGCGTGA CCAGTCAACA 
ATTCCACGCA ATCTCCTGAA GACAGATGCT GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT 
GCACAATTCA CTGTTCTTTC TAAGCTGAGA ACCCCACTGG GCAGAGCTCA AGACACCTTC 
CAGACAATTG AAGGTATCAT TCGAAGTCTC GCAGCTCACA CATTAAACCC TGATCAGGAT 
GTTAGTCAGT GGACAACTGC AGACAATGAT GAAGGCCATG GTAACAACCA ACTTAGACTT 
GTTCTTCTTC TGCAGTATCT GGAAAATCTG GAGAAATTAA TGTATAATGC ATACGAGGGA 
TGTGCTAATG CATTAACTTC ACCTCCCAAG GTCATTAGAA CTTTTTTCTA TACCAATCGC 
CAAACTTGTC AGGACTGGCT AACGCGGATT CGACTCTCCA TCATGAGGGT AGGATTGTTG 
GCAGGCCAGC CTGCAGTGAC AGTGAGACAT GGCTTTGACT TGCTTACAGA GATGAAAACA 
ACCAGCCTAT CTCAGGGGAA TGAATTGGAA GTAACCATTA TGATGGTGGT AGAAGCATTA 
TGTGAACTTC ATTGTCCTGA AGCTATACAG GGAATTGCTG TCTGGTCATC ATCTATTGTT 
GGAAAAAATC TTCTGTGGAT TAACTCAGTG GCTCAACAGG CTGAAGGGAG GTTTGAAAAG 
GCCTCTGTGG AGTACCAGGA ACACCTGTGT GCCATGACAG GTGTTGATTG CTGCATCTCC 
AGCTTTGACA AATCGGTGCT CACCTTAGCC AATGCTGGGC GTAACAGTGC CAGCCCGAAA 
CATTCTCTGA ATGGTGAATC CAGAAAAACT GTGCTGTCCA AACCGACTGA CTCTTCCCCT 
GAGGTTATAA ATTATTTAGG AAATAAAGCA TGTGAGTGCT ACATCTCAAT TGCCGATTGG 
GCTGCTGTGC AGGAATGGCA GAACGCTATC CATGACTTGA AAAAGAGTAC CAGTAGCACT 
TCCCTCAACC TGAAAGCTGA CTTCAACTAT ATAAAATCAT TAAGCAGCTT TGAGTCTGGA 
AAATTTGTTG AATGTACCGA GCAGTTAGAA TTGTTACCAG GAGAAAATAT CAATCTACTT 
GCTGGAGGAT CAAAAGAAAA AATAGACATG AAAAAACTGC TTCCTAACAT GTTAAGTCCG 
GATCCGAGGG AACTTCAGAA ATCCATTGAA GTTCAATTGT TAAGAAGTTC TGTTTGTTTG 
GCAACTGCTT TAAACCCGAT AGAACAAGAT CAGAAGTGGC AGTCTATAAC TGAAAATGTG 
GTAAAGTACT TGAAGCAAAC ATCCCGCATC GCTATTGGAC CTCTGAGACT TTCTACTTTA 
ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT ACCTTGCAGC TGTATTGCTC ATCTGCTTTG 
GAGAACACAG TTTCTAACAG ACTTTCAACA GAGGACTGTC TTATTCCACT CTTCAGTGAA 
GCTTTACGTT CATGTAAACA GCATGACGTG AGGCCATGGA TGCAGGCATT AAGGTATACT 
ATGTACCAGA ATCAGTTGTT GGAGAAAATT AAAGAACAAA CAGTCCCAAT TAGAAGCCAT 
CTCATGGAAT TAGGTCTAAC AGCAGCAAAA TTTGCTAGAA AACGAGGGAA TGTGTCCCTT 
GCAACAAGAC TGCTGGCACA GTGCAGTGAA GTTCAGCTGG GAAAGACCAC CACTGCACAG 
GATTTAGTCC AACATTTTAA AAAACTATCA ACCCAAGGTC AAGTGGATGA AAAATGGGGG 
CCCGAACTTG ATATTGAAAA AACCAAATTG CTTTATACAG CAGGCCAGTC AACACATGCA 
ATGGAAATGT TGAGTTCTTG TGCCATATCT TTCTGCAAGT CTGTGAAAGC TGAATATGCA 
GTTGCTAAAT CAATTCTGAC ACTGGCTAAA TGGATCCAGG CAGAATGGAA AGAGATTTCA 
GGACAGCTGA AACAGGTTTA CAGAGCTCAG CACCAACAGA ACTTCACAGG TCTTTCTACT 
TTGTCTAAAA ACATACTCAC TCTAATAGAA CTGCCATCTG TTAATACGAT GGAAGAAGAG 
TATCCTCGGA TCGAGAGTGA ATCTACAGTG CATATTGGAG TTGGAGAACC TGACTTCATT 
TTGGGACAGT TGTATCACCT GTCTTCAGTA CAGGCACCTG AAGTAGCCAA ATCTTGGGCA 
GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC AGAAAGGTGG TTGACAATGC CAGTCAGGGA 
GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA TCTGAAGTTC AGAATCTACT TCCAGACACT 
ATAACTGAGG AAGAGAAAGA GAGAATATAT GGTATTCTTG GACAGGCTGT GTGTCGGCCG 
GCGGGGATTC AGGATGAAGA TATAACACTT CAGATAACTG AGAGTGAAGA CAACGAAGAA 
GATGACATGG TTGATGTTAT CTGGCGTCAG TTGATATCAA GCTGCCCATG GCTTTCAGAA 
CTTGATGAAA GTGCAACTGA AGGAGTTATT AAAGTGTGGA GGAAAGTTGT AGATAGAATA 
TTCAGCCTGT ACAAACTCTC TTGCAGTGCA TACTTTACTT TCCTTAAACT CAACGCTGGT 
CAAATTCCTT TAGATGAGGA TGACCCTAGG CTGCATTTAA GTCACAGAGT GGAACAGAGC 
ACTGATGACA TGATTGTGAT GGCCACATTG CGCCTGCTGC GGTTGCTCGT GAAGCATGCT 
GGTGAGCTTC GGCAGTATCT GGAGCACGGC TTGGAGACAA CACCCACTGC ACCATGGAGA 
GGAATTATTC CGCAACTTTT CTCACGCTTA AACCACCCTG AAGTGTATGT GCGCCAAAGT 
ATTTGTAACC TTCTCTGCCG TGTGGCTCAA GATTCCCCAC ATCTCATATT GTATCCTGCA 
ATAGTGGGTA CCATATCGCT TAGTAGTGAA TCCCAGGCTT CAGGAAATAA ATTTTCCACT 
GCAATTCCAA CTTTACTTGG CAATATTCAA GGAGAAGAAT TGCTGGTTTC TGAATGTGAG 
GGAGGAAGTC CTCCTGCATC TCAGGATAGC AATAAGGATG AACCTAAAAG TGGATTAAAT 
GAAGACCAAG CCATGATGCA GGATTGTTAC AGCAAAATTG TAGATAAGCT GTCCTCTGCA 
AACCCCACCA TGGTATTACA GGTTCAGATG CTCGTGGCTG AACTGCGCAG GGTCACTGTG 
CTCTGGGATG AGCTCTGGCT GGGAGTTTTG CTGCAACAAC ACATGTATGT CCTGAGACGA 
ATTCAGCAGC TTGAAGATGA GGTGAAGAGA GTCCAGAACA ACAACACCTT ACGCAAAGAA 
GAGAAAATTG CAATCATGAG GGAGAAGCAC ACAGCTTTGA TGAAGCCCAT CGTATTTGCT 
TTGGAGCATG TGAGGAGTAT CACAGCGGCT CCTGCAGAAA CACCTCATGA AAAATGGTTT 
CAGGATAACT ATGGTGATGC CATTGAAAAT GCCCTAGAAA AACTGAAGAC TCCATTGAAC 
CCTGCAAAGC CTGGGAGCAG CTGGATTCCA TTTAAAGAGA TAATGCTAAG TTTGCAACAG 
AGAGCACAGA AACGTGCAAG TTACATCTTG CGTCTTGAAG AAATCAGTCC ATGGTTGGCT 
GCCATGACTA ACACTGAAAT TGCTCTTCCT GGGGAAGTCT CAGCCAGAGA CACTGTCACA 
ATCCATAGTG TGGGCGGAAC CATCACAATC TTACCGACTA AAACCAAGCC AAAGAAACTT 
CTCTTTCTTG GATCAGATGG GAAGAGCTAT CCTTATCTTT TCAAAGGACT GGAGGATTTA 
CATCTGGATG AGAGAATAAT GCAGTTCCTA TCTATTGTGA ATACCATGTT TGCTACAATT 
AATCGCCAAG AAACACCCCG GTTCCATGCT CGACACTATT CTGTAACACC ACTAGGAACA 
AGATCAGGAC TAATCCAGTG GGTAGATGGA GCCACACCCT TATTTGGTCT TTACAAACGA 
TGGCAACAAC GGGAAGCTGC CTTACAAGCA CAAAAGGCCC AAGATTCCTA CCAAACTCCT 
CAGAATCCTG GAATTGTACC CCGTCCTAGT GAACTTTATT ACAGTAAAAT TGGCCCTGCT 
TTGAAAACAG TTGGGCTTAG CCTGGATGTG TCCCGTCGGG ATTGGCCTCT TCATGTAATG 
AAGGCAGTAT TGGAAGAGTT AATGGAGGCC ACACCCCCGA ATCTCCTTGC CAAAGAGCTC 
TGGTCATCTT GCACAACACC TGATGAATGG TGGAGAGTTA CGCAGTCTTA TGCAAGATCT 
ACTGCAGTCA TGTCTATGGT TGGATACATA ATTGGCCTTG GAGACAGACA TCTGGATAAT 
GTTCTTATAG ATATGACGAC TGGAGAAGTT GTTCACATAG ATTACAATGT TTGCTTTGAA 
AAAGGTAATA TAAAAGTAGA CTTTACATTT ATGAGGGCCA AATGTTTTTT GGTGAAAGGG 
CAAAAATATA ATGATTTGTC TTTTCAAGGT AAAAGCCTTA GAGTTCCTGA GAAAGTACCT 
TTTCGAATGA CACAAAACAT TGAAACAGCA CTGGGTGTAA CTGGAGTAGA AGGTGTATTT 
AGGCTTTCAT GTGAGCAGGT TTTACACATT ATGCGGCGTG GCAGAGAGAC CCTGCTGACG 
CTGCTGGAGG CCTTTGTGTA CGACCCTCTG GTGGACTGGA CAGCAGGAGG CGAGGCTGGG 
TTTGCTGGTG CTGTCTATGG TGGAGGTGGC CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA 
GAGATGGAGC GAGAGATCAC CCGCAGCCTG TTTTCTTCTA GAGTAGCTGA GATTAAGGTG 
AACTGGTTTA AGAATAGAGA TGAGATGCTG GTTGTGCTTC CCAAGTTGGA CGGTAGCTTA 
GATGAATACC TAAGCTTGCA AGAGCAACTG ACAGATGTGG AAAAACTGCA GGGCAAACTA 
CTGGAGGAAA TAGAGTTTCT AGAAGGAGCT GAAGGGGTGG ATCATCCTTC TCATACTCTG 
CAACACAGGT ATTCTGAGCA CACCCAACTA CAGACTCAGC AAAGAGCTGT TCAGGAAGCA 
ATCCAGGTGA AGCTGAATGA ATTTGAACAA TGGATAACAC ATTATCAGGC TGCATTCAAT 
AATTTAGAAG CAACACAGCT TGCAAGCTTG CTTCAAGAGA TAAGCACACA AATGGACCTT 
GGTCCTCCAA GTTACGTGCC AGCAACAGCC TTTCTGCAGA ATGCTGGTCA GGCCCACTTG 
ATTAGCCAGT GCGAGCAGCT GGAGGGGGAG GTTGGTGCTC TCCTGCAGCA GAGGCGCTCC 
GTGCTCCGTG GCTGTCTGGA GCAACTGCAT CACTATGCAA CCGTGGCCCT GCAGTATCCG 
AAGGCCATAT TTCAGAAACA TCGAATTGAA CAGTGGAAGA CCTGGATGGA AGAGCTCATC 
TGTAACACCA CAGTAGAGCG TTGTCAAGAG CTCTATAGGA AATATGAAAT GCAATATGCT 
CCCCAGCCAC CCCCAACAGT GTGTCAGTTC ATCACTGCCA CTGAAATGAC CCTGCAGCGA 
TACGCAGCAG ACATCAACAG CAGACTTATT AGACAAGTGG AACGCTTGAA ACAGGAAGCT 
GTCACTGTGC CAGTTTGTGA AGATCAGTTG AAAGAAATTG AACGTTGCAT TAAAGTTTTC 
CTTCATGAGA ATGGAGAAGA AGGATCTTTG AGTCTAGCAA GTGTTATTAT TTCTGCCCTT 
TGTACCCTTA CAAGGCGTAA CCTGATGATG GAAGGTGCAG CGTCAAGTGC TGGAGAACAG 
CTGGTTGATC TGACTTCTCG GGATGGAGCC TGGTTCTTGG AGGAACTCTG CAGTATGAGC 
GGAAACGTCA CCTGCTTGGT TCAGTTACTG AAGCAGTGCC ACCTGGTGCC ACAGGACTTA 
GATATCCCGA ACCCCATGGA AGCGTCTGAG ACAGTTCACT TAGCCAATGG AGTGTATACC 
TCACTTCAGG AATTGAATTC GAATTTCCGG CAAATCATAT TTCCAGAAGC ACTTCGATGT 
TTAATGAAAG GGGAATACAC GTTAGAAAGT ATGCTGCATG AACTGGACGG TCTTATTGAG 
CAGACCACCG ATGGCGTTCC CCTGCAGACT CTAGTGGAAT CTCTTCAGGC CTACTTAAGA 
AACGCAGCTA TGGGACTGGA AGAAGAAACA CATGCTCATT ACATCGATGT TGCCAGACTA 
CTACATGCTC AGTACGGTGA ATTAATCCAA CCGAGAAATG GTTCAGTTGA TGAAACACCC 
AAAATGTCAG CTGGCCAGAT GCTTTTGGTA GCATTCGATG GCATGTTTGC TCAAGTTGAA 
ACTGCTTTCA GCTTATTAGT TGAAAAGTTG AACAAGATGG AAATTCCCAT AGCTTGGCGA 
AAGATTGACA TCATAAGGGA AGCCAGGAGT ACTCAAGTTA ATTTTTTTGA TGATGATAAT 
CACCGGCAGG TGCTAGAAGA GATTTTCTTT CTAAAAAGAC TACAGACTAT TAAGGAGTTC 
TTCAGGCTCT GTGGTACCTT TTCTAAAACA TTGTCAGGAT CAAGTTCACT TGAAGATCAG 
AATACTGTGA ATGGGCCTGT ACAGATTGTC AATGTGAAAA CCCTTTTTAG AAACTCTTGT 
TTCAGTGAAG ACCAAATGGC CAAACCTATC AAGGCATTCA CAGCTGACTT TGTGAGGCAG 
CTCTTGATAG GGCTACCCAA CCAAGCCCTC GGACTCACAC TGTGCAGTTT TATCAGTGCT 
CTGGGTGTAG ACATCATTGC TCAAGTAGAG GCAAAGGACT TTGGTGCCGA AAGCAAAGTT 
TCTGTTGATG ATCTCTGTAA GAAAGCGGTG GAACATAACA TCCAGATAGG GAAGTTCTCT 
CAGCTGGTTA TGAACAGGGC AACTGTGTTA GCAAGTTCTT ACGACACTGC CTGGAAGAAG 
CATGACTTGG TGCGAAGGCT AGAAACCAGT ATTTCTTCTT GTAAGACAAG CCTGCAGCGG 
GTTCAGCTGC ATATTGCCAT GTTTCAGTGG CAACATGAAG ATCTACTTAT CAATAGACCA 
CAAGCCATGT CAGTCACACC TCCCCCACGG TCTGCTATCC TAACCAGCAT GAAAAAGAAG 
CTGCATACCC TGAGCCAGAT TGAAACTTCT ATTGCAACAG TTCAGGAGAA GCTAGCTGCA 
CTTGAATCAA GTATTGAACA GCGACTCAAG TGGGCAGGTG GTGCCAACCC TGCATTGGCC 
CCTGTACTAC AAGATTTTGA AGCAACGATA GCTGAAAGAA GAAATCTTGT CCTTAAAGAG 
AGCCAAAGAG CAAGTCAGGT CACATTTCTC TGCAGCAATA TCATTCATTT TGAAAGTTTA 
CGAACAAGAA CTGCAGAAGC CTTAAACCTG GATGCGGCGT TATTTGAACT AATCAAGCGA 
TGTCAGCAGA TGTGTTCGTT TGCATCACAG TTTAACAGTT CAGTGTCTGA GTTAGAGCTT 
CGTTTATTAC AGAGAGTGGA CACTGGTCTT GAACATCCTA TTGGCAGCTC TGAATGGCTT 
TTGTCAGCAC ACAAACAGTT GACCCAGGAT ATGTCTACTC AGAGGGCAAT TCAGACAGAG 
AAAGAGCAGC AGATAGAAAC GGTCTGTGAA ACAATTCAGA ATCTGGTTGA TAATATAAAG 
ACTGTGCTCA CTGGTCATAA CCGACAGCTT GGAGATGTCA AACATCTCTT GAAAGCTATG 
GCTAAGGATG AAGAAGCTGC TCTGGCAGAT GGTGAAGATG TTCCCTATGA GAACAGTGTT 
AGGCAGTTTT TGGGTGAATA TAAATCATGG CAAGACAACA TTCAAACAGT TCTATTTACA 
TTAGTCCAGG CTATGGGTCA GGTTCGAAGT CAAGAACACG TTGAAATGCT CCAGGAAATC 
ACTCCCACCT TGAAAGAACT GAAAACACAA AGTCAGAGTA TCTATAATAA TTTAGTGAGT 
TTTGCATCAC CCTTAGTCAC CGATGCAACA AATGAATGTT CGAGTCCAAC GTCATCTGCT 
ACTTATCAGC CATCCTTCGC TGCAGTCCGG AGTAACACTG GCCAGAAGAC TCAGCCTGAT 
GTCATGTCAC AGAATGCTAG AAAGCTGATC CAAAAAAATC TTGCTACATC AGCTGATACT 
CCACCAAGCA CCGTTCCAGG AACTGGCAAG AGTGTTGCTT GTAGTCCTAA AAAGGCAGTC 
AGAGACCCTA AAACTGGGAA AGCGGTGCAA GAGAGAAACT CCTATGCAGT GAGTGTGTGG 
AAGAGAGTGA AAGCCAAGTT AGAGGGCCGA GATGTTGATC CGAATAGGAG GATGTCAGTT 
GCTGAACAGG TTGACTATGT CATTAAGGAA GCAACTAATC TAGATAACTT GGCTCAGCTG 
TATGAAGGTT GGACAGCCTG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544071.1
CDS	377..11443
Translation	MSRRAPGSRLSSGGGGGGTKYPRSWNDWQPRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSVQHGSRALATKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGNIKVDFTFMRAKCFLVKGQKYNDLSFQGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_011545769.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1, nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545769.2

ORF Insert Sequence:

ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCGG CGGCACCAAG 
TATCCGCGGA GCTGGAATGA CTGGCAACCC AGAACTGATA GTGCATCAGC CGACCCAGAT 
AATTTAAAAT ATTCTTCATC CAGAGATAGA GGTGGTTCTT CCTCTTATGG ACTGCAACCT 
TCAAATTCAG CTGTGGTGTC TCGGCAAAGG CACGATGATA CCAGAGTCCA CGCTGACATA 
CAGAATGACG AAAAGGGTGG CTACAGTGTC AATGGAGGAT CTGGGGAAAA TACTTATGGT 
CGGAAGTCGT TGGGGCAAGA GCTGAGGGTT AACAATGTGA CCAGCCCTGA GTTCACCAGT 
GTTCAGCATG GCAGTCGTGC TTTAGCCACC AAAGACATGA GGAAATCACA GGAGAGATCG 
ATGTCTTATT CTGATGAGTC TCGACTGTCG AATCTTCTTC GGAGGATCAC CCGGGAAGAC 
GACAGAGACC GAAGATTGGC TACTGTAAAG CAGTTGAAAG AATTTATTCA GCAACCAGAA 
AATAAGCTGG TACTAGTTAA ACAATTGGAT AATATCTTGG CTGCTGTACA TGACGTGCTT 
AATGAAAGTA GCAAATTGCT TCAGGAGTTG AGACAGGAGG GAGCTTGCTG TCTTGGCCTT 
CTTTGTGCTT CTCTGAGCTA TGAGGCTGAG AAGATCTTCA AGTGGATTTT TAGCAAATTT 
AGCTCATCTG CAAAAGATGA AGTTAAACTC CTCTACTTAT GTGCCACCTA CAAAGCACTA 
GAGACTGTAG GAGAAAAGAA AGCCTTTTCA TCTGTAATGC AGCTTGTAAT GACCAGCCTG 
CAGTCTATTC TTGAAAATGT GGATACACCA GAATTGCTTT GTAAATGTGT TAAGTGCATT 
CTTTTGGTGG CTCGATGTTA CCCTCATATT TTCAGCACTA ATTTTAGGGA TACAGTTGAT 
ATATTAGTTG GATGGCATAT AGATCATACT CAGAAACCTT CGCTCACGCA GCAGGTATCT 
GGGTGGTTGC AGAGTTTGGA GCCATTTTGG GTAGCTGATC TTGCATTTTC TACTACTCTT 
CTTGGTCAGT TTCTGGAAGA CATGGAAGCA TATGCTGAGG ACCTCAGCCA TGTGGCCTCT 
GGGGAATCAG TGGATGAAGA TGTCCCTCCT CCATCAGTGT CATTACCAAA GCTGGCTGCA 
CTTCTCCGGG TATTTAGTAC TGTGGTGAGG AGCATTGGGG AACGCTTCAG CCCAATTCGG 
GGTCCTCCAA TTACTGAGGC ATATGTAACA GATGTTCTGT ACAGAGTAAT GAGATGTGTG 
ACGGCTGCAA ACCAGGTGTT TTTTTCTGAG GCTGTGTTGA CAGCTGCTAA TGAGTGTGTT 
GGTGTTTTGC TCGGCAGCTT GGATCCTAGC ATGACTATAC ATTGTGACAT GGTCATTACA 
TATGGATTAG ACCAACTGGA GAATTGCCAG ACTTGTGGTA CCGATTATAT CATCTCAGTC 
TTGAATTTAC TCACGCTGAT TGTTGAACAG ATAAATACGA AACTGCCATC ATCATTTGTA 
GAAAAACTGT TTATACCATC ATCTAAACTA CTATTCTTGC GTTATCATAA AGAAAAAGAG 
GTTGTTGCTG TAGCCCATGC TGTTTATCAA GCAGTGCTCA GCTTGAAGAA TATTCCTGTT 
TTGGAGACTG CCTATAAGTT AATATTGGGA GAAATGACTT GTGCCCTAAA CAACCTCCTA 
CACAGTCTAC AACTTCCTGA GGCCTGTTCT GAAATAAAAC ATGAGGCTTT TAAGAATCAT 
GTGTTCAATG TAGACAATGC AAAATTTGTA GTTATATTTG ACCTCAGTGC CCTGACTACA 
ATTGGAAATG CCAAAAACTC ACTAATAGGG ATGTGGGCGC TATCTCCAAC TGTCTTTGCA 
CTTCTGAGTA AGAATCTGAT GATTGTGCAC AGTGACCTGG CTGTTCACTT CCCTGCCATT 
CAGTATGCTG TGCTCTACAC ATTGTATTCT CATTGTACCA GGCATGATCA CTTTATCTCT 
AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG TTTGATGGAG CTGTGATTAG CACTGTAACT 
ACGGCTACAA AGAAACATTT CTCAATTATA TTAAATCTTC TGGGAATATT ACTTAAGAAA 
GATAACCTTA ACCAGGACAC GAGGAAACTG TTAATGACTT GGGCTTTGGA AGCAGCTGTT 
TTAATGAAGA AGTCTGAAAC ATACGCACCT TTATTCTCTC TTCCGTCTTT CCATAAATTT 
TGCAAAGGCC TTTTAGCCAA CACTCTCGTT GAAGATGTGA ATATCTGTCT GCAGGCATGC 
AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG CCAGATGATC TTTTACAGAG ATGTGTCGAT 
GTTTGCCGTG TTCAACTAGT GCACAGTGGA ACTCGTATTC GACAAGCATT TGGAAAACTG 
TTGAAATCAA TTCCTTTAGA TGTTGTCCTA AGCAATAACA ATCACACAGA AATTCAAGAA 
ATTTCTTTAG CATTAAGAAG TCACATGAGT AAAGCACCAA GTAATACATT CCACCCCCAA 
GATTTCTCTG ATGTTATTAG TTTTATTTTG TATGGGAACT CTCATAGAAC AGGGAAGGAC 
AATTGGTTGG AAAGACTGTT CTATAGCTGC CAGAGACTGG ATAAGCGTGA CCAGTCAACA 
ATTCCACGCA ATCTCCTGAA GACAGATGCT GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT 
GCACAATTCA CTGTTCTTTC TAAGCTGAGA ACCCCACTGG GCAGAGCTCA AGACACCTTC 
CAGACAATTG AAGGTATCAT TCGAAGTCTC GCAGCTCACA CATTAAACCC TGATCAGGAT 
GTTAGTCAGT GGACAACTGC AGACAATGAT GAAGGCCATG GTAACAACCA ACTTAGACTT 
GTTCTTCTTC TGCAGTATCT GGAAAATCTG GAGAAATTAA TGTATAATGC ATACGAGGGA 
TGTGCTAATG CATTAACTTC ACCTCCCAAG GTCATTAGAA CTTTTTTCTA TACCAATCGC 
CAAACTTGTC AGGACTGGCT AACGCGGATT CGACTCTCCA TCATGAGGGT AGGATTGTTG 
GCAGGCCAGC CTGCAGTGAC AGTGAGACAT GGCTTTGACT TGCTTACAGA GATGAAAACA 
ACCAGCCTAT CTCAGGGGAA TGAATTGGAA GTAACCATTA TGATGGTGGT AGAAGCATTA 
TGTGAACTTC ATTGTCCTGA AGCTATACAG GGAATTGCTG TCTGGTCATC ATCTATTGTT 
GGAAAAAATC TTCTGTGGAT TAACTCAGTG GCTCAACAGG CTGAAGGGAG GTTTGAAAAG 
GCCTCTGTGG AGTACCAGGA ACACCTGTGT GCCATGACAG GTGTTGATTG CTGCATCTCC 
AGCTTTGACA AATCGGTGCT CACCTTAGCC AATGCTGGGC GTAACAGTGC CAGCCCGAAA 
CATTCTCTGA ATGGTGAATC CAGAAAAACT GTGCTGTCCA AACCGACTGA CTCTTCCCCT 
GAGGTTATAA ATTATTTAGG AAATAAAGCA TGTGAGTGCT ACATCTCAAT TGCCGATTGG 
GCTGCTGTGC AGGAATGGCA GAACGCTATC CATGACTTGA AAAAGAGTAC CAGTAGCACT 
TCCCTCAACC TGAAAGCTGA CTTCAACTAT ATAAAATCAT TAAGCAGCTT TGAGTCTGGA 
AAATTTGTTG AATGTACCGA GCAGTTAGAA TTGTTACCAG GAGAAAATAT CAATCTACTT 
GCTGGAGGAT CAAAAGAAAA AATAGACATG AAAAAACTGC TTCCTAACAT GTTAAGTCCG 
GATCCGAGGG AACTTCAGAA ATCCATTGAA GTTCAATTGT TAAGAAGTTC TGTTTGTTTG 
GCAACTGCTT TAAACCCGAT AGAACAAGAT CAGAAGTGGC AGTCTATAAC TGAAAATGTG 
GTAAAGTACT TGAAGCAAAC ATCCCGCATC GCTATTGGAC CTCTGAGACT TTCTACTTTA 
ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT ACCTTGCAGC TGTATTGCTC ATCTGCTTTG 
GAGAACACAG TTTCTAACAG ACTTTCAACA GAGGACTGTC TTATTCCACT CTTCAGTGAA 
GCTTTACGTT CATGTAAACA GCATGACGTG AGGCCATGGA TGCAGGCATT AAGGTATACT 
ATGTACCAGA ATCAGTTGTT GGAGAAAATT AAAGAACAAA CAGTCCCAAT TAGAAGCCAT 
CTCATGGAAT TAGGTCTAAC AGCAGCAAAA TTTGCTAGAA AACGAGGGAA TGTGTCCCTT 
GCAACAAGAC TGCTGGCACA GTGCAGTGAA GTTCAGCTGG GAAAGACCAC CACTGCACAG 
GATTTAGTCC AACATTTTAA AAAACTATCA ACCCAAGGTC AAGTGGATGA AAAATGGGGG 
CCCGAACTTG ATATTGAAAA AACCAAATTG CTTTATACAG CAGGCCAGTC AACACATGCA 
ATGGAAATGT TGAGTTCTTG TGCCATATCT TTCTGCAAGT CTGTGAAAGC TGAATATGCA 
GTTGCTAAAT CAATTCTGAC ACTGGCTAAA TGGATCCAGG CAGAATGGAA AGAGATTTCA 
GGACAGCTGA AACAGGTTTA CAGAGCTCAG CACCAACAGA ACTTCACAGG TCTTTCTACT 
TTGTCTAAAA ACATACTCAC TCTAATAGAA CTGCCATCTG TTAATACGAT GGAAGAAGAG 
TATCCTCGGA TCGAGAGTGA ATCTACAGTG CATATTGGAG TTGGAGAACC TGACTTCATT 
TTGGGACAGT TGTATCACCT GTCTTCAGTA CAGGCACCTG AAGTAGCCAA ATCTTGGGCA 
GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC AGAAAGGTGG TTGACAATGC CAGTCAGGGA 
GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA TCTGAAGTTC AGAATCTACT TCCAGACACT 
ATAACTGAGG AAGAGAAAGA GAGAATATAT GGTATTCTTG GACAGGCTGT GTGTCGGCCG 
GCGGGGATTC AGGATGAAGA TATAACACTT CAGATAACTG AGAGTGAAGA CAACGAAGAA 
GATGACATGG TTGATGTTAT CTGGCGTCAG TTGATATCAA GCTGCCCATG GCTTTCAGAA 
CTTGATGAAA GTGCAACTGA AGGAGTTATT AAAGTGTGGA GGAAAGTTGT AGATAGAATA 
TTCAGCCTGT ACAAACTCTC TTGCAGTGCA TACTTTACTT TCCTTAAACT CAACGCTGGT 
CAAATTCCTT TAGATGAGGA TGACCCTAGG CTGCATTTAA GTCACAGAGT GGAACAGAGC 
ACTGATGACA TGATTGTGAT GGCCACATTG CGCCTGCTGC GGTTGCTCGT GAAGCATGCT 
GGTGAGCTTC GGCAGTATCT GGAGCACGGC TTGGAGACAA CACCCACTGC ACCATGGAGA 
GGAATTATTC CGCAACTTTT CTCACGCTTA AACCACCCTG AAGTGTATGT GCGCCAAAGT 
ATTTGTAACC TTCTCTGCCG TGTGGCTCAA GATTCCCCAC ATCTCATATT GTATCCTGCA 
ATAGTGGGTA CCATATCGCT TAGTAGTGAA TCCCAGGCTT CAGGAAATAA ATTTTCCACT 
GCAATTCCAA CTTTACTTGG CAATATTCAA GGAGAAGAAT TGCTGGTTTC TGAATGTGAG 
GGAGGAAGTC CTCCTGCATC TCAGGATAGC AATAAGGATG AACCTAAAAG TGGATTAAAT 
GAAGACCAAG CCATGATGCA GGATTGTTAC AGCAAAATTG TAGATAAGCT GTCCTCTGCA 
AACCCCACCA TGGTATTACA GGTTCAGATG CTCGTGGCTG AACTGCGCAG GGTCACTGTG 
CTCTGGGATG AGCTCTGGCT GGGAGTTTTG CTGCAACAAC ACATGTATGT CCTGAGACGA 
ATTCAGCAGC TTGAAGATGA GGTGAAGAGA GTCCAGAACA ACAACACCTT ACGCAAAGAA 
GAGAAAATTG CAATCATGAG GGAGAAGCAC ACAGCTTTGA TGAAGCCCAT CGTATTTGCT 
TTGGAGCATG TGAGGAGTAT CACAGCGGCT CCTGCAGAAA CACCTCATGA AAAATGGTTT 
CAGGATAACT ATGGTGATGC CATTGAAAAT GCCCTAGAAA AACTGAAGAC TCCATTGAAC 
CCTGCAAAGC CTGGGAGCAG CTGGATTCCA TTTAAAGAGA TAATGCTAAG TTTGCAACAG 
AGAGCACAGA AACGTGCAAG TTACATCTTG CGTCTTGAAG AAATCAGTCC ATGGTTGGCT 
GCCATGACTA ACACTGAAAT TGCTCTTCCT GGGGAAGTCT CAGCCAGAGA CACTGTCACA 
ATCCATAGTG TGGGCGGAAC CATCACAATC TTACCGACTA AAACCAAGCC AAAGAAACTT 
CTCTTTCTTG GATCAGATGG GAAGAGCTAT CCTTATCTTT TCAAAGGACT GGAGGATTTA 
CATCTGGATG AGAGAATAAT GCAGTTCCTA TCTATTGTGA ATACCATGTT TGCTACAATT 
AATCGCCAAG AAACACCCCG GTTCCATGCT CGACACTATT CTGTAACACC ACTAGGAACA 
AGATCAGGAC TAATCCAGTG GGTAGATGGA GCCACACCCT TATTTGGTCT TTACAAACGA 
TGGCAACAAC GGGAAGCTGC CTTACAAGCA CAAAAGGCCC AAGATTCCTA CCAAACTCCT 
CAGAATCCTG GAATTGTACC CCGTCCTAGT GAACTTTATT ACAGTAAAAT TGGCCCTGCT 
TTGAAAACAG TTGGGCTTAG CCTGGATGTG TCCCGTCGGG ATTGGCCTCT TCATGTAATG 
AAGGCAGTAT TGGAAGAGTT AATGGAGGCC ACACCCCCGA ATCTCCTTGC CAAAGAGCTC 
TGGTCATCTT GCACAACACC TGATGAATGG TGGAGAGTTA CGCAGTCTTA TGCAAGATCT 
ACTGCAGTCA TGTCTATGGT TGGATACATA ATTGGCCTTG GAGACAGACA TCTGGATAAT 
GTTCTTATAG ATATGACGAC TGGAGAAGTT GTTCACATAG ATTACAATGT TTGCTTTGAA 
AAAGGTAATA TAAAAGTAGA CTTTACATTT ATGAGGGCCA AATGTTTTTT GGTGAAAGGG 
CAAAAATATA ATGATTTGTC TTTTCAAGGT AAAAGCCTTA GAGTTCCTGA GAAAGTACCT 
TTTCGAATGA CACAAAACAT TGAAACAGCA CTGGGTGTAA CTGGAGTAGA AGGTGTATTT 
AGGCTTTCAT GTGAGCAGGT TTTACACATT ATGCGGCGTG GCAGAGAGAC CCTGCTGACG 
CTGCTGGAGG CCTTTGTGTA CGACCCTCTG GTGGACTGGA CAGCAGGAGG CGAGGCTGGG 
TTTGCTGGTG CTGTCTATGG TGGAGGTGGC CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA 
GAGATGGAGC GAGAGATCAC CCGCAGCCTG TTTTCTTCTA GAGTAGCTGA GATTAAGGTG 
AACTGGTTTA AGAATAGAGA TGAGATGCTG GTTGTGCTTC CCAAGTTGGA CGGTAGCTTA 
GATGAATACC TAAGCTTGCA AGAGCAACTG ACAGATGTGG AAAAACTGCA GGGCAAACTA 
CTGGAGGAAA TAGAGTTTCT AGAAGGAGCT GAAGGGGTGG ATCATCCTTC TCATACTCTG 
CAACACAGGT ATTCTGAGCA CACCCAACTA CAGACTCAGC AAAGAGCTGT TCAGGAAGCA 
ATCCAGGTGA AGCTGAATGA ATTTGAACAA TGGATAACAC ATTATCAGGC TGCATTCAAT 
AATTTAGAAG CAACACAGCT TGCAAGCTTG CTTCAAGAGA TAAGCACACA AATGGACCTT 
GGTCCTCCAA GTTACGTGCC AGCAACAGCC TTTCTGCAGA ATGCTGGTCA GGCCCACTTG 
ATTAGCCAGT GCGAGCAGCT GGAGGGGGAG GTTGGTGCTC TCCTGCAGCA GAGGCGCTCC 
GTGCTCCGTG GCTGTCTGGA GCAACTGCAT CACTATGCAA CCGTGGCCCT GCAGTATCCG 
AAGGCCATAT TTCAGAAACA TCGAATTGAA CAGTGGAAGA CCTGGATGGA AGAGCTCATC 
TGTAACACCA CAGTAGAGCG TTGTCAAGAG CTCTATAGGA AATATGAAAT GCAATATGCT 
CCCCAGCCAC CCCCAACAGT GTGTCAGTTC ATCACTGCCA CTGAAATGAC CCTGCAGCGA 
TACGCAGCAG ACATCAACAG CAGACTTATT AGACAAGTGG AACGCTTGAA ACAGGAAGCT 
GTCACTGTGC CAGTTTGTGA AGATCAGTTG AAAGAAATTG AACGTTGCAT TAAAGTTTTC 
CTTCATGAGA ATGGAGAAGA AGGATCTTTG AGTCTAGCAA GTGTTATTAT TTCTGCCCTT 
TGTACCCTTA CAAGGCGTAA CCTGATGATG GAAGGTGCAG CGTCAAGTGC TGGAGAACAG 
CTGGTTGATC TGACTTCTCG GGATGGAGCC TGGTTCTTGG AGGAACTCTG CAGTATGAGC 
GGAAACGTCA CCTGCTTGGT TCAGTTACTG AAGCAGTGCC ACCTGGTGCC ACAGGACTTA 
GATATCCCGA ACCCCATGGA AGCGTCTGAG ACAGTTCACT TAGCCAATGG AGTGTATACC 
TCACTTCAGG AATTGAATTC GAATTTCCGG CAAATCATAT TTCCAGAAGC ACTTCGATGT 
TTAATGAAAG GGGAATACAC GTTAGAAAGT ATGCTGCATG AACTGGACGG TCTTATTGAG 
CAGACCACCG ATGGCGTTCC CCTGCAGACT CTAGTGGAAT CTCTTCAGGC CTACTTAAGA 
AACGCAGCTA TGGGACTGGA AGAAGAAACA CATGCTCATT ACATCGATGT TGCCAGACTA 
CTACATGCTC AGTACGGTGA ATTAATCCAA CCGAGAAATG GTTCAGTTGA TGAAACACCC 
AAAATGTCAG CTGGCCAGAT GCTTTTGGTA GCATTCGATG GCATGTTTGC TCAAGTTGAA 
ACTGCTTTCA GCTTATTAGT TGAAAAGTTG AACAAGATGG AAATTCCCAT AGCTTGGCGA 
AAGATTGACA TCATAAGGGA AGCCAGGAGT ACTCAAGTTA ATTTTTTTGA TGATGATAAT 
CACCGGCAGG TGCTAGAAGA GATTTTCTTT CTAAAAAGAC TACAGACTAT TAAGGAGTTC 
TTCAGGCTCT GTGGTACCTT TTCTAAAACA TTGTCAGGAT CAAGTTCACT TGAAGATCAG 
AATACTGTGA ATGGGCCTGT ACAGATTGTC AATGTGAAAA CCCTTTTTAG AAACTCTTGT 
TTCAGTGAAG ACCAAATGGC CAAACCTATC AAGGCATTCA CAGCTGACTT TGTGAGGCAG 
CTCTTGATAG GGCTACCCAA CCAAGCCCTC GGACTCACAC TGTGCAGTTT TATCAGTGCT 
CTGGGTGTAG ACATCATTGC TCAAGTAGAG GCAAAGGACT TTGGTGCCGA AAGCAAAGTT 
TCTGTTGATG ATCTCTGTAA GAAAGCGGTG GAACATAACA TCCAGATAGG GAAGTTCTCT 
CAGCTGGTTA TGAACAGGGC AACTGTGTTA GCAAGTTCTT ACGACACTGC CTGGAAGAAG 
CATGACTTGG TGCGAAGGCT AGAAACCAGT ATTTCTTCTT GTAAGACAAG CCTGCAGCGG 
GTTCAGCTGC ATATTGCCAT GTTTCAGTGG CAACATGAAG ATCTACTTAT CAATAGACCA 
CAAGCCATGT CAGTCACACC TCCCCCACGG TCTGCTATCC TAACCAGCAT GAAAAAGAAG 
CTGCATACCC TGAGCCAGAT TGAAACTTCT ATTGCAACAG TTCAGGAGAA GCTAGCTGCA 
CTTGAATCAA GTATTGAACA GCGACTCAAG TGGGCAGGTG GTGCCAACCC TGCATTGGCC 
CCTGTACTAC AAGATTTTGA AGCAACGATA GCTGAAAGAA GAAATCTTGT CCTTAAAGAG 
AGCCAAAGAG CAAGTCAGGT CACATTTCTC TGCAGCAATA TCATTCATTT TGAAAGTTTA 
CGAACAAGAA CTGCAGAAGC CTTAAACCTG GATGCGGCGT TATTTGAACT AATCAAGCGA 
TGTCAGCAGA TGTGTTCGTT TGCATCACAG TTTAACAGTT CAGTGTCTGA GTTAGAGCTT 
CGTTTATTAC AGAGAGTGGA CACTGGTCTT GAACATCCTA TTGGCAGCTC TGAATGGCTT 
TTGTCAGCAC ACAAACAGTT GACCCAGGAT ATGTCTACTC AGAGGGCAAT TCAGACAGAG 
AAAGAGCAGC AGATAGAAAC GGTCTGTGAA ACAATTCAGA ATCTGGTTGA TAATATAAAG 
ACTGTGCTCA CTGGTCATAA CCGACAGCTT GGAGATGTCA AACATCTCTT GAAAGCTATG 
GCTAAGGATG AAGAAGCTGC TCTGGCAGAT GGTGAAGATG TTCCCTATGA GAACAGTGTT 
AGGCAGTTTT TGGGTGAATA TAAATCATGG CAAGACAACA TTCAAACAGT TCTATTTACA 
TTAGTCCAGG CTATGGGTCA GGTTCGAAGT CAAGAACACG TTGAAATGCT CCAGGAAATC 
ACTCCCACCT TGAAAGAACT GAAAACACAA AGTCAGAGTA TCTATAATAA TTTAGTGAGT 
TTTGCATCAC CCTTAGTCAC CGATGCAACA AATGAATGTT CGAGTCCAAC GTCATCTGCT 
ACTTATCAGC CATCCTTCGC TGCAGTCCGG AGTAACACTG GCCAGAAGAC TCAGCCTGAT 
GTCATGTCAC AGAATGCTAG AAAGCTGATC CAAAAAAATC TTGCTACATC AGCTGATACT 
CCACCAAGCA CCGTTCCAGG AACTGGCAAG AGTGTTGCTT GTAGTCCTAA AAAGGCAGTC 
AGAGACCCTA AAACTGGGAA AGCGGTGCAA GAGAGAAACT CCTATGCAGT GAGTGTGTGG 
AAGAGAGTGA AAGCCAAGTT AGAGGGCCGA GATGTTGATC CGAATAGGAG GATGTCAGTT 
GCTGAACAGG TTGACTATGT CATTAAGGAA GCAACTAATC TAGATAACTT GGCTCAGCTG 
TATGAAGGTT GGACAGCCTG GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_011545770.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544072.1 Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X8, mRNA.

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CloneID	OHu65358
Clone ID Related Accession (Same CDS sequence)	XM_011545770.1 , XM_017023067.1
Accession Version	XM_011545770.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCGC TATCTCCAAC TGTCTTTGCA CTTCTGAGTA AGAATCTGAT GATTGTGCAC 
AGTGACCTGG CTGTTCACTT CCCTGCCATT CAGTATGCTG TGCTCTACAC ATTGTATTCT 
CATTGTACCA GGCATGATCA CTTTATCTCT AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG 
TTTGATGGAG CTGTGATTAG CACTGTAACT ACGGCTACAA AGAAACATTT CTCAATTATA 
TTAAATCTTC TGGGAATATT ACTTAAGAAA GATAACCTTA ACCAGGACAC GAGGAAACTG 
TTAATGACTT GGGCTTTGGA AGCAGCTGTT TTAATGAAGA AGTCTGAAAC ATACGCACCT 
TTATTCTCTC TTCCGTCTTT CCATAAATTT TGCAAAGGCC TTTTAGCCAA CACTCTCGTT 
GAAGATGTGA ATATCTGTCT GCAGGCATGC AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG 
CCAGATGATC TTTTACAGAG ATGTGTCGAT GTTTGCCGTG TTCAACTAGT GCACAGTGGA 
ACTCGTATTC GACAAGCATT TGGAAAACTG TTGAAATCAA TTCCTTTAGA TGTTGTCCTA 
AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT 
AAAGCACCAA GTAATACATT CCACCCCCAA GATTTCTCTG ATGTTATTAG TTTTATTTTG 
TATGGGAACT CTCATAGAAC AGGGAAGGAC AATTGGTTGG AAAGACTGTT CTATAGCTGC 
CAGAGACTGG ATAAGCGTGA CCAGTCAACA ATTCCACGCA ATCTCCTGAA GACAGATGCT 
GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT GCACAATTCA CTGTTCTTTC TAAGCTGAGA 
ACCCCACTGG GCAGAGCTCA AGACACCTTC CAGACAATTG AAGGTATCAT TCGAAGTCTC 
GCAGCTCACA CATTAAACCC TGATCAGGAT GTTAGTCAGT GGACAACTGC AGACAATGAT 
GAAGGCCATG GTAACAACCA ACTTAGACTT GTTCTTCTTC TGCAGTATCT GGAAAATCTG 
GAGAAATTAA TGTATAATGC ATACGAGGGA TGTGCTAATG CATTAACTTC ACCTCCCAAG 
GTCATTAGAA CTTTTTTCTA TACCAATCGC CAAACTTGTC AGGACTGGCT AACGCGGATT 
CGACTCTCCA TCATGAGGGT AGGATTGTTG GCAGGCCAGC CTGCAGTGAC AGTGAGACAT 
GGCTTTGACT TGCTTACAGA GATGAAAACA ACCAGCCTAT CTCAGGGGAA TGAATTGGAA 
GTAACCATTA TGATGGTGGT AGAAGCATTA TGTGAACTTC ATTGTCCTGA AGCTATACAG 
GGAATTGCTG TCTGGTCATC ATCTATTGTT GGAAAAAATC TTCTGTGGAT TAACTCAGTG 
GCTCAACAGG CTGAAGGGAG GTTTGAAAAG GCCTCTGTGG AGTACCAGGA ACACCTGTGT 
GCCATGACAG GTGTTGATTG CTGCATCTCC AGCTTTGACA AATCGGTGCT CACCTTAGCC 
AATGCTGGGC GTAACAGTGC CAGCCCGAAA CATTCTCTGA ATGGTGAATC CAGAAAAACT 
GTGCTGTCCA AACCGACTGA CTCTTCCCCT GAGGTTATAA ATTATTTAGG AAATAAAGCA 
TGTGAGTGCT ACATCTCAAT TGCCGATTGG GCTGCTGTGC AGGAATGGCA GAACGCTATC 
CATGACTTGA AAAAGAGTAC CAGTAGCACT TCCCTCAACC TGAAAGCTGA CTTCAACTAT 
ATAAAATCAT TAAGCAGCTT TGAGTCTGGA AAATTTGTTG AATGTACCGA GCAGTTAGAA 
TTGTTACCAG GAGAAAATAT CAATCTACTT GCTGGAGGAT CAAAAGAAAA AATAGACATG 
AAAAAACTGC TTCCTAACAT GTTAAGTCCG GATCCGAGGG AACTTCAGAA ATCCATTGAA 
GTTCAATTGT TAAGAAGTTC TGTTTGTTTG GCAACTGCTT TAAACCCGAT AGAACAAGAT 
CAGAAGTGGC AGTCTATAAC TGAAAATGTG GTAAAGTACT TGAAGCAAAC ATCCCGCATC 
GCTATTGGAC CTCTGAGACT TTCTACTTTA ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT 
ACCTTGCAGC TGTATTGCTC ATCTGCTTTG GAGAACACAG TTTCTAACAG ACTTTCAACA 
GAGGACTGTC TTATTCCACT CTTCAGTGAA GCTTTACGTT CATGTAAACA GCATGACGTG 
AGGCCATGGA TGCAGGCATT AAGGTATACT ATGTACCAGA ATCAGTTGTT GGAGAAAATT 
AAAGAACAAA CAGTCCCAAT TAGAAGCCAT CTCATGGAAT TAGGTCTAAC AGCAGCAAAA 
TTTGCTAGAA AACGAGGGAA TGTGTCCCTT GCAACAAGAC TGCTGGCACA GTGCAGTGAA 
GTTCAGCTGG GAAAGACCAC CACTGCACAG GATTTAGTCC AACATTTTAA AAAACTATCA 
ACCCAAGGTC AAGTGGATGA AAAATGGGGG CCCGAACTTG ATATTGAAAA AACCAAATTG 
CTTTATACAG CAGGCCAGTC AACACATGCA ATGGAAATGT TGAGTTCTTG TGCCATATCT 
TTCTGCAAGT CTGTGAAAGC TGAATATGCA GTTGCTAAAT CAATTCTGAC ACTGGCTAAA 
TGGATCCAGG CAGAATGGAA AGAGATTTCA GGACAGCTGA AACAGGTTTA CAGAGCTCAG 
CACCAACAGA ACTTCACAGG TCTTTCTACT TTGTCTAAAA ACATACTCAC TCTAATAGAA 
CTGCCATCTG TTAATACGAT GGAAGAAGAG TATCCTCGGA TCGAGAGTGA ATCTACAGTG 
CATATTGGAG TTGGAGAACC TGACTTCATT TTGGGACAGT TGTATCACCT GTCTTCAGTA 
CAGGCACCTG AAGTAGCCAA ATCTTGGGCA GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC 
AGAAAGGTGG TTGACAATGC CAGTCAGGGA GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA 
TCTGAAGTTC AGAATCTACT TCCAGACACT ATAACTGAGG AAGAGAAAGA GAGAATATAT 
GGTATTCTTG GACAGGCTGT GTGTCGGCCG GCGGGGATTC AGGATGAAGA TATAACACTT 
CAGATAACTG AGAGTGAAGA CAACGAAGAA GATGACATGG TTGATGTTAT CTGGCGTCAG 
TTGATATCAA GCTGCCCATG GCTTTCAGAA CTTGATGAAA GTGCAACTGA AGGAGTTATT 
AAAGTGTGGA GGAAAGTTGT AGATAGAATA TTCAGCCTGT ACAAACTCTC TTGCAGTGCA 
TACTTTACTT TCCTTAAACT CAACGCTGGT CAAATTCCTT TAGATGAGGA TGACCCTAGG 
CTGCATTTAA GTCACAGAGT GGAACAGAGC ACTGATGACA TGATTGTGAT GGCCACATTG 
CGCCTGCTGC GGTTGCTCGT GAAGCATGCT GGTGAGCTTC GGCAGTATCT GGAGCACGGC 
TTGGAGACAA CACCCACTGC ACCATGGAGA GGAATTATTC CGCAACTTTT CTCACGCTTA 
AACCACCCTG AAGTGTATGT GCGCCAAAGT ATTTGTAACC TTCTCTGCCG TGTGGCTCAA 
GATTCCCCAC ATCTCATATT GTATCCTGCA ATAGTGGGTA CCATATCGCT TAGTAGTGAA 
TCCCAGGCTT CAGGAAATAA ATTTTCCACT GCAATTCCAA CTTTACTTGG CAATATTCAA 
GGAGAAGAAT TGCTGGTTTC TGAATGTGAG GGAGGAAGTC CTCCTGCATC TCAGGATAGC 
AATAAGGATG AACCTAAAAG TGGATTAAAT GAAGACCAAG CCATGATGCA GGATTGTTAC 
AGCAAAATTG TAGATAAGCT GTCCTCTGCA AACCCCACCA TGGTATTACA GGTTCAGATG 
CTCGTGGCTG AACTGCGCAG GGTCACTGTG CTCTGGGATG AGCTCTGGCT GGGAGTTTTG 
CTGCAACAAC ACATGTATGT CCTGAGACGA ATTCAGCAGC TTGAAGATGA GGTGAAGAGA 
GTCCAGAACA ACAACACCTT ACGCAAAGAA GAGAAAATTG CAATCATGAG GGAGAAGCAC 
ACAGCTTTGA TGAAGCCCAT CGTATTTGCT TTGGAGCATG TGAGGAGTAT CACAGCGGCT 
CCTGCAGAAA CACCTCATGA AAAATGGTTT CAGGATAACT ATGGTGATGC CATTGAAAAT 
GCCCTAGAAA AACTGAAGAC TCCATTGAAC CCTGCAAAGC CTGGGAGCAG CTGGATTCCA 
TTTAAAGAGA TAATGCTAAG TTTGCAACAG AGAGCACAGA AACGTGCAAG TTACATCTTG 
CGTCTTGAAG AAATCAGTCC ATGGTTGGCT GCCATGACTA ACACTGAAAT TGCTCTTCCT 
GGGGAAGTCT CAGCCAGAGA CACTGTCACA ATCCATAGTG TGGGCGGAAC CATCACAATC 
TTACCGACTA AAACCAAGCC AAAGAAACTT CTCTTTCTTG GATCAGATGG GAAGAGCTAT 
CCTTATCTTT TCAAAGGACT GGAGGATTTA CATCTGGATG AGAGAATAAT GCAGTTCCTA 
TCTATTGTGA ATACCATGTT TGCTACAATT AATCGCCAAG AAACACCCCG GTTCCATGCT 
CGACACTATT CTGTAACACC ACTAGGAACA AGATCAGGAC TAATCCAGTG GGTAGATGGA 
GCCACACCCT TATTTGGTCT TTACAAACGA TGGCAACAAC GGGAAGCTGC CTTACAAGCA 
CAAAAGGCCC AAGATTCCTA CCAAACTCCT CAGAATCCTG GAATTGTACC CCGTCCTAGT 
GAACTTTATT ACAGTAAAAT TGGCCCTGCT TTGAAAACAG TTGGGCTTAG CCTGGATGTG 
TCCCGTCGGG ATTGGCCTCT TCATGTAATG AAGGCAGTAT TGGAAGAGTT AATGGAGGCC 
ACACCCCCGA ATCTCCTTGC CAAAGAGCTC TGGTCATCTT GCACAACACC TGATGAATGG 
TGGAGAGTTA CGCAGTCTTA TGCAAGATCT ACTGCAGTCA TGTCTATGGT TGGATACATA 
ATTGGCCTTG GAGACAGACA TCTGGATAAT GTTCTTATAG ATATGACGAC TGGAGAAGTT 
GTTCACATAG ATTACAATGT TTGCTTTGAA AAAGGTAATA TAAAAGTAGA CTTTACATTT 
ATGAGGGCCA AATGTTTTTT GGTGAAAGGG CAAAAATATA ATGATTTGTC TTTTCAAGGT 
AAAAGCCTTA GAGTTCCTGA GAAAGTACCT TTTCGAATGA CACAAAACAT TGAAACAGCA 
CTGGGTGTAA CTGGAGTAGA AGGTGTATTT AGGCTTTCAT GTGAGCAGGT TTTACACATT 
ATGCGGCGTG GCAGAGAGAC CCTGCTGACG CTGCTGGAGG CCTTTGTGTA CGACCCTCTG 
GTGGACTGGA CAGCAGGAGG CGAGGCTGGG TTTGCTGGTG CTGTCTATGG TGGAGGTGGC 
CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA GAGATGGAGC GAGAGATCAC CCGCAGCCTG 
TTTTCTTCTA GAGTAGCTGA GATTAAGGTG AACTGGTTTA AGAATAGAGA TGAGATGCTG 
GTTGTGCTTC CCAAGTTGGA CGGTAGCTTA GATGAATACC TAAGCTTGCA AGAGCAACTG 
ACAGATGTGG AAAAACTGCA GGGCAAACTA CTGGAGGAAA TAGAGTTTCT AGAAGGAGCT 
GAAGGGGTGG ATCATCCTTC TCATACTCTG CAACACAGGT ATTCTGAGCA CACCCAACTA 
CAGACTCAGC AAAGAGCTGT TCAGGAAGCA ATCCAGGTGA AGCTGAATGA ATTTGAACAA 
TGGATAACAC ATTATCAGGC TGCATTCAAT AATTTAGAAG CAACACAGCT TGCAAGCTTG 
CTTCAAGAGA TAAGCACACA AATGGACCTT GGTCCTCCAA GTTACGTGCC AGCAACAGCC 
TTTCTGCAGA ATGCTGGTCA GGCCCACTTG ATTAGCCAGT GCGAGCAGCT GGAGGGGGAG 
GTTGGTGCTC TCCTGCAGCA GAGGCGCTCC GTGCTCCGTG GCTGTCTGGA GCAACTGCAT 
CACTATGCAA CCGTGGCCCT GCAGTATCCG AAGGCCATAT TTCAGAAACA TCGAATTGAA 
CAGTGGAAGA CCTGGATGGA AGAGCTCATC TGTAACACCA CAGTAGAGCG TTGTCAAGAG 
CTCTATAGGA AATATGAAAT GCAATATGCT CCCCAGCCAC CCCCAACAGT GTGTCAGTTC 
ATCACTGCCA CTGAAATGAC CCTGCAGCGA TACGCAGCAG ACATCAACAG CAGACTTATT 
AGACAAGTGG AACGCTTGAA ACAGGAAGCT GTCACTGTGC CAGTTTGTGA AGATCAGTTG 
AAAGAAATTG AACGTTGCAT TAAAGTTTTC CTTCATGAGA ATGGAGAAGA AGGATCTTTG 
AGTCTAGCAA GTGTTATTAT TTCTGCCCTT TGTACCCTTA CAAGGCGTAA CCTGATGATG 
GAAGGTGCAG CGTCAAGTGC TGGAGAACAG CTGGTTGATC TGACTTCTCG GGATGGAGCC 
TGGTTCTTGG AGGAACTCTG CAGTATGAGC GGAAACGTCA CCTGCTTGGT TCAGTTACTG 
AAGCAGTGCC ACCTGGTGCC ACAGGACTTA GATATCCCGA ACCCCATGGA AGCGTCTGAG 
ACAGTTCACT TAGCCAATGG AGTGTATACC TCACTTCAGG AATTGAATTC GAATTTCCGG 
CAAATCATAT TTCCAGAAGC ACTTCGATGT TTAATGAAAG GGGAATACAC GTTAGAAAGT 
ATGCTGCATG AACTGGACGG TCTTATTGAG CAGACCACCG ATGGCGTTCC CCTGCAGACT 
CTAGTGGAAT CTCTTCAGGC CTACTTAAGA AACGCAGCTA TGGGACTGGA AGAAGAAACA 
CATGCTCATT ACATCGATGT TGCCAGACTA CTACATGCTC AGTACGGTGA ATTAATCCAA 
CCGAGAAATG GTTCAGTTGA TGAAACACCC AAAATGTCAG CTGGCCAGAT GCTTTTGGTA 
GCATTCGATG GCATGTTTGC TCAAGTTGAA ACTGCTTTCA GCTTATTAGT TGAAAAGTTG 
AACAAGATGG AAATTCCCAT AGCTTGGCGA AAGATTGACA TCATAAGGGA AGCCAGGAGT 
ACTCAAGTTA ATTTTTTTGA TGATGATAAT CACCGGCAGG TGCTAGAAGA GATTTTCTTT 
CTAAAAAGAC TACAGACTAT TAAGGAGTTC TTCAGGCTCT GTGGTACCTT TTCTAAAACA 
TTGTCAGGAT CAAGTTCACT TGAAGATCAG AATACTGTGA ATGGGCCTGT ACAGATTGTC 
AATGTGAAAA CCCTTTTTAG AAACTCTTGT TTCAGTGAAG ACCAAATGGC CAAACCTATC 
AAGGCATTCA CAGCTGACTT TGTGAGGCAG CTCTTGATAG GGCTACCCAA CCAAGCCCTC 
GGACTCACAC TGTGCAGTTT TATCAGTGCT CTGGGTGTAG ACATCATTGC TCAAGTAGAG 
GCAAAGGACT TTGGTGCCGA AAGCAAAGTT TCTGTTGATG ATCTCTGTAA GAAAGCGGTG 
GAACATAACA TCCAGATAGG GAAGTTCTCT CAGCTGGTTA TGAACAGGGC AACTGTGTTA 
GCAAGTTCTT ACGACACTGC CTGGAAGAAG CATGACTTGG TGCGAAGGCT AGAAACCAGT 
ATTTCTTCTT GTAAGACAAG CCTGCAGCGG GTTCAGCTGC ATATTGCCAT GTTTCAGTGG 
CAACATGAAG ATCTACTTAT CAATAGACCA CAAGCCATGT CAGTCACACC TCCCCCACGG 
TCTGCTATCC TAACCAGCAT GAAAAAGAAG CTGCATACCC TGAGCCAGAT TGAAACTTCT 
ATTGCAACAG TTCAGGAGAA GCTAGCTGCA CTTGAATCAA GTATTGAACA GCGACTCAAG 
TGGGCAGGTG GTGCCAACCC TGCATTGGCC CCTGTACTAC AAGATTTTGA AGCAACGATA 
GCTGAAAGAA GAAATCTTGT CCTTAAAGAG AGCCAAAGAG CAAGTCAGGT CACATTTCTC 
TGCAGCAATA TCATTCATTT TGAAAGTTTA CGAACAAGAA CTGCAGAAGC CTTAAACCTG 
GATGCGGCGT TATTTGAACT AATCAAGCGA TGTCAGCAGA TGTGTTCGTT TGCATCACAG 
TTTAACAGTT CAGTGTCTGA GTTAGAGCTT CGTTTATTAC AGAGAGTGGA CACTGGTCTT 
GAACATCCTA TTGGCAGCTC TGAATGGCTT TTGTCAGCAC ACAAACAGTT GACCCAGGAT 
ATGTCTACTC AGAGGGCAAT TCAGACAGAG AAAGAGCAGC AGATAGAAAC GGTCTGTGAA 
ACAATTCAGA ATCTGGTTGA TAATATAAAG ACTGTGCTCA CTGGTCATAA CCGACAGCTT 
GGAGATGTCA AACATCTCTT GAAAGCTATG GCTAAGGATG AAGAAGCTGC TCTGGCAGAT 
GGTGAAGATG TTCCCTATGA GAACAGTGTT AGGCAGTTTT TGGGTGAATA TAAATCATGG 
CAAGACAACA TTCAAACAGT TCTATTTACA TTAGTCCAGG CTATGGGTCA GGTTCGAAGT 
CAAGAACACG TTGAAATGCT CCAGGAAATC ACTCCCACCT TGAAAGAACT GAAAACACAA 
AGTCAGAGTA TCTATAATAA TTTAGTGAGT TTTGCATCAC CCTTAGTCAC CGATGCAACA 
AATGAATGTT CGAGTCCAAC GTCATCTGCT ACTTATCAGC CATCCTTCGC TGCAGCAGTC 
CGGAGTAACA CTGGCCAGAA GACTCAGCCT GATGTCATGT CACAGAATGC TAGAAAGCTG 
ATCCAAAAAA ATCTTGCTAC ATCAGCTGAT ACTCCACCAA GCACCGTTCC AGGAACTGGC 
AAGAGTGTTG CTTGTAGTCC TAAAAAGGCA GTCAGAGACC CTAAAACTGG GAAAGCGGTG 
CAAGAGAGAA ACTCCTATGC AGTGAGTGTG TGGAAGAGAG TGAAAGCCAA GTTAGAGGGC 
CGAGATGTTG ATCCGAATAG GAGGATGTCA GTTGCTGAAC AGGTTGACTA TGTCATTAAG 
GAAGCAACTA ATCTAGATAA CTTGGCTCAG CTGTATGAAG GTTGGACAGC CTGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544072.1
CDS	64..9243
Translation	MWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGNIKVDFTFMRAKCFLVKGQKYNDLSFQGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_011545770.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545770.1

ORF Insert Sequence:

ATGTGGGCGC TATCTCCAAC TGTCTTTGCA CTTCTGAGTA AGAATCTGAT GATTGTGCAC 
AGTGACCTGG CTGTTCACTT CCCTGCCATT CAGTATGCTG TGCTCTACAC ATTGTATTCT 
CATTGTACCA GGCATGATCA CTTTATCTCT AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG 
TTTGATGGAG CTGTGATTAG CACTGTAACT ACGGCTACAA AGAAACATTT CTCAATTATA 
TTAAATCTTC TGGGAATATT ACTTAAGAAA GATAACCTTA ACCAGGACAC GAGGAAACTG 
TTAATGACTT GGGCTTTGGA AGCAGCTGTT TTAATGAAGA AGTCTGAAAC ATACGCACCT 
TTATTCTCTC TTCCGTCTTT CCATAAATTT TGCAAAGGCC TTTTAGCCAA CACTCTCGTT 
GAAGATGTGA ATATCTGTCT GCAGGCATGC AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG 
CCAGATGATC TTTTACAGAG ATGTGTCGAT GTTTGCCGTG TTCAACTAGT GCACAGTGGA 
ACTCGTATTC GACAAGCATT TGGAAAACTG TTGAAATCAA TTCCTTTAGA TGTTGTCCTA 
AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT 
AAAGCACCAA GTAATACATT CCACCCCCAA GATTTCTCTG ATGTTATTAG TTTTATTTTG 
TATGGGAACT CTCATAGAAC AGGGAAGGAC AATTGGTTGG AAAGACTGTT CTATAGCTGC 
CAGAGACTGG ATAAGCGTGA CCAGTCAACA ATTCCACGCA ATCTCCTGAA GACAGATGCT 
GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT GCACAATTCA CTGTTCTTTC TAAGCTGAGA 
ACCCCACTGG GCAGAGCTCA AGACACCTTC CAGACAATTG AAGGTATCAT TCGAAGTCTC 
GCAGCTCACA CATTAAACCC TGATCAGGAT GTTAGTCAGT GGACAACTGC AGACAATGAT 
GAAGGCCATG GTAACAACCA ACTTAGACTT GTTCTTCTTC TGCAGTATCT GGAAAATCTG 
GAGAAATTAA TGTATAATGC ATACGAGGGA TGTGCTAATG CATTAACTTC ACCTCCCAAG 
GTCATTAGAA CTTTTTTCTA TACCAATCGC CAAACTTGTC AGGACTGGCT AACGCGGATT 
CGACTCTCCA TCATGAGGGT AGGATTGTTG GCAGGCCAGC CTGCAGTGAC AGTGAGACAT 
GGCTTTGACT TGCTTACAGA GATGAAAACA ACCAGCCTAT CTCAGGGGAA TGAATTGGAA 
GTAACCATTA TGATGGTGGT AGAAGCATTA TGTGAACTTC ATTGTCCTGA AGCTATACAG 
GGAATTGCTG TCTGGTCATC ATCTATTGTT GGAAAAAATC TTCTGTGGAT TAACTCAGTG 
GCTCAACAGG CTGAAGGGAG GTTTGAAAAG GCCTCTGTGG AGTACCAGGA ACACCTGTGT 
GCCATGACAG GTGTTGATTG CTGCATCTCC AGCTTTGACA AATCGGTGCT CACCTTAGCC 
AATGCTGGGC GTAACAGTGC CAGCCCGAAA CATTCTCTGA ATGGTGAATC CAGAAAAACT 
GTGCTGTCCA AACCGACTGA CTCTTCCCCT GAGGTTATAA ATTATTTAGG AAATAAAGCA 
TGTGAGTGCT ACATCTCAAT TGCCGATTGG GCTGCTGTGC AGGAATGGCA GAACGCTATC 
CATGACTTGA AAAAGAGTAC CAGTAGCACT TCCCTCAACC TGAAAGCTGA CTTCAACTAT 
ATAAAATCAT TAAGCAGCTT TGAGTCTGGA AAATTTGTTG AATGTACCGA GCAGTTAGAA 
TTGTTACCAG GAGAAAATAT CAATCTACTT GCTGGAGGAT CAAAAGAAAA AATAGACATG 
AAAAAACTGC TTCCTAACAT GTTAAGTCCG GATCCGAGGG AACTTCAGAA ATCCATTGAA 
GTTCAATTGT TAAGAAGTTC TGTTTGTTTG GCAACTGCTT TAAACCCGAT AGAACAAGAT 
CAGAAGTGGC AGTCTATAAC TGAAAATGTG GTAAAGTACT TGAAGCAAAC ATCCCGCATC 
GCTATTGGAC CTCTGAGACT TTCTACTTTA ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT 
ACCTTGCAGC TGTATTGCTC ATCTGCTTTG GAGAACACAG TTTCTAACAG ACTTTCAACA 
GAGGACTGTC TTATTCCACT CTTCAGTGAA GCTTTACGTT CATGTAAACA GCATGACGTG 
AGGCCATGGA TGCAGGCATT AAGGTATACT ATGTACCAGA ATCAGTTGTT GGAGAAAATT 
AAAGAACAAA CAGTCCCAAT TAGAAGCCAT CTCATGGAAT TAGGTCTAAC AGCAGCAAAA 
TTTGCTAGAA AACGAGGGAA TGTGTCCCTT GCAACAAGAC TGCTGGCACA GTGCAGTGAA 
GTTCAGCTGG GAAAGACCAC CACTGCACAG GATTTAGTCC AACATTTTAA AAAACTATCA 
ACCCAAGGTC AAGTGGATGA AAAATGGGGG CCCGAACTTG ATATTGAAAA AACCAAATTG 
CTTTATACAG CAGGCCAGTC AACACATGCA ATGGAAATGT TGAGTTCTTG TGCCATATCT 
TTCTGCAAGT CTGTGAAAGC TGAATATGCA GTTGCTAAAT CAATTCTGAC ACTGGCTAAA 
TGGATCCAGG CAGAATGGAA AGAGATTTCA GGACAGCTGA AACAGGTTTA CAGAGCTCAG 
CACCAACAGA ACTTCACAGG TCTTTCTACT TTGTCTAAAA ACATACTCAC TCTAATAGAA 
CTGCCATCTG TTAATACGAT GGAAGAAGAG TATCCTCGGA TCGAGAGTGA ATCTACAGTG 
CATATTGGAG TTGGAGAACC TGACTTCATT TTGGGACAGT TGTATCACCT GTCTTCAGTA 
CAGGCACCTG AAGTAGCCAA ATCTTGGGCA GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC 
AGAAAGGTGG TTGACAATGC CAGTCAGGGA GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA 
TCTGAAGTTC AGAATCTACT TCCAGACACT ATAACTGAGG AAGAGAAAGA GAGAATATAT 
GGTATTCTTG GACAGGCTGT GTGTCGGCCG GCGGGGATTC AGGATGAAGA TATAACACTT 
CAGATAACTG AGAGTGAAGA CAACGAAGAA GATGACATGG TTGATGTTAT CTGGCGTCAG 
TTGATATCAA GCTGCCCATG GCTTTCAGAA CTTGATGAAA GTGCAACTGA AGGAGTTATT 
AAAGTGTGGA GGAAAGTTGT AGATAGAATA TTCAGCCTGT ACAAACTCTC TTGCAGTGCA 
TACTTTACTT TCCTTAAACT CAACGCTGGT CAAATTCCTT TAGATGAGGA TGACCCTAGG 
CTGCATTTAA GTCACAGAGT GGAACAGAGC ACTGATGACA TGATTGTGAT GGCCACATTG 
CGCCTGCTGC GGTTGCTCGT GAAGCATGCT GGTGAGCTTC GGCAGTATCT GGAGCACGGC 
TTGGAGACAA CACCCACTGC ACCATGGAGA GGAATTATTC CGCAACTTTT CTCACGCTTA 
AACCACCCTG AAGTGTATGT GCGCCAAAGT ATTTGTAACC TTCTCTGCCG TGTGGCTCAA 
GATTCCCCAC ATCTCATATT GTATCCTGCA ATAGTGGGTA CCATATCGCT TAGTAGTGAA 
TCCCAGGCTT CAGGAAATAA ATTTTCCACT GCAATTCCAA CTTTACTTGG CAATATTCAA 
GGAGAAGAAT TGCTGGTTTC TGAATGTGAG GGAGGAAGTC CTCCTGCATC TCAGGATAGC 
AATAAGGATG AACCTAAAAG TGGATTAAAT GAAGACCAAG CCATGATGCA GGATTGTTAC 
AGCAAAATTG TAGATAAGCT GTCCTCTGCA AACCCCACCA TGGTATTACA GGTTCAGATG 
CTCGTGGCTG AACTGCGCAG GGTCACTGTG CTCTGGGATG AGCTCTGGCT GGGAGTTTTG 
CTGCAACAAC ACATGTATGT CCTGAGACGA ATTCAGCAGC TTGAAGATGA GGTGAAGAGA 
GTCCAGAACA ACAACACCTT ACGCAAAGAA GAGAAAATTG CAATCATGAG GGAGAAGCAC 
ACAGCTTTGA TGAAGCCCAT CGTATTTGCT TTGGAGCATG TGAGGAGTAT CACAGCGGCT 
CCTGCAGAAA CACCTCATGA AAAATGGTTT CAGGATAACT ATGGTGATGC CATTGAAAAT 
GCCCTAGAAA AACTGAAGAC TCCATTGAAC CCTGCAAAGC CTGGGAGCAG CTGGATTCCA 
TTTAAAGAGA TAATGCTAAG TTTGCAACAG AGAGCACAGA AACGTGCAAG TTACATCTTG 
CGTCTTGAAG AAATCAGTCC ATGGTTGGCT GCCATGACTA ACACTGAAAT TGCTCTTCCT 
GGGGAAGTCT CAGCCAGAGA CACTGTCACA ATCCATAGTG TGGGCGGAAC CATCACAATC 
TTACCGACTA AAACCAAGCC AAAGAAACTT CTCTTTCTTG GATCAGATGG GAAGAGCTAT 
CCTTATCTTT TCAAAGGACT GGAGGATTTA CATCTGGATG AGAGAATAAT GCAGTTCCTA 
TCTATTGTGA ATACCATGTT TGCTACAATT AATCGCCAAG AAACACCCCG GTTCCATGCT 
CGACACTATT CTGTAACACC ACTAGGAACA AGATCAGGAC TAATCCAGTG GGTAGATGGA 
GCCACACCCT TATTTGGTCT TTACAAACGA TGGCAACAAC GGGAAGCTGC CTTACAAGCA 
CAAAAGGCCC AAGATTCCTA CCAAACTCCT CAGAATCCTG GAATTGTACC CCGTCCTAGT 
GAACTTTATT ACAGTAAAAT TGGCCCTGCT TTGAAAACAG TTGGGCTTAG CCTGGATGTG 
TCCCGTCGGG ATTGGCCTCT TCATGTAATG AAGGCAGTAT TGGAAGAGTT AATGGAGGCC 
ACACCCCCGA ATCTCCTTGC CAAAGAGCTC TGGTCATCTT GCACAACACC TGATGAATGG 
TGGAGAGTTA CGCAGTCTTA TGCAAGATCT ACTGCAGTCA TGTCTATGGT TGGATACATA 
ATTGGCCTTG GAGACAGACA TCTGGATAAT GTTCTTATAG ATATGACGAC TGGAGAAGTT 
GTTCACATAG ATTACAATGT TTGCTTTGAA AAAGGTAATA TAAAAGTAGA CTTTACATTT 
ATGAGGGCCA AATGTTTTTT GGTGAAAGGG CAAAAATATA ATGATTTGTC TTTTCAAGGT 
AAAAGCCTTA GAGTTCCTGA GAAAGTACCT TTTCGAATGA CACAAAACAT TGAAACAGCA 
CTGGGTGTAA CTGGAGTAGA AGGTGTATTT AGGCTTTCAT GTGAGCAGGT TTTACACATT 
ATGCGGCGTG GCAGAGAGAC CCTGCTGACG CTGCTGGAGG CCTTTGTGTA CGACCCTCTG 
GTGGACTGGA CAGCAGGAGG CGAGGCTGGG TTTGCTGGTG CTGTCTATGG TGGAGGTGGC 
CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA GAGATGGAGC GAGAGATCAC CCGCAGCCTG 
TTTTCTTCTA GAGTAGCTGA GATTAAGGTG AACTGGTTTA AGAATAGAGA TGAGATGCTG 
GTTGTGCTTC CCAAGTTGGA CGGTAGCTTA GATGAATACC TAAGCTTGCA AGAGCAACTG 
ACAGATGTGG AAAAACTGCA GGGCAAACTA CTGGAGGAAA TAGAGTTTCT AGAAGGAGCT 
GAAGGGGTGG ATCATCCTTC TCATACTCTG CAACACAGGT ATTCTGAGCA CACCCAACTA 
CAGACTCAGC AAAGAGCTGT TCAGGAAGCA ATCCAGGTGA AGCTGAATGA ATTTGAACAA 
TGGATAACAC ATTATCAGGC TGCATTCAAT AATTTAGAAG CAACACAGCT TGCAAGCTTG 
CTTCAAGAGA TAAGCACACA AATGGACCTT GGTCCTCCAA GTTACGTGCC AGCAACAGCC 
TTTCTGCAGA ATGCTGGTCA GGCCCACTTG ATTAGCCAGT GCGAGCAGCT GGAGGGGGAG 
GTTGGTGCTC TCCTGCAGCA GAGGCGCTCC GTGCTCCGTG GCTGTCTGGA GCAACTGCAT 
CACTATGCAA CCGTGGCCCT GCAGTATCCG AAGGCCATAT TTCAGAAACA TCGAATTGAA 
CAGTGGAAGA CCTGGATGGA AGAGCTCATC TGTAACACCA CAGTAGAGCG TTGTCAAGAG 
CTCTATAGGA AATATGAAAT GCAATATGCT CCCCAGCCAC CCCCAACAGT GTGTCAGTTC 
ATCACTGCCA CTGAAATGAC CCTGCAGCGA TACGCAGCAG ACATCAACAG CAGACTTATT 
AGACAAGTGG AACGCTTGAA ACAGGAAGCT GTCACTGTGC CAGTTTGTGA AGATCAGTTG 
AAAGAAATTG AACGTTGCAT TAAAGTTTTC CTTCATGAGA ATGGAGAAGA AGGATCTTTG 
AGTCTAGCAA GTGTTATTAT TTCTGCCCTT TGTACCCTTA CAAGGCGTAA CCTGATGATG 
GAAGGTGCAG CGTCAAGTGC TGGAGAACAG CTGGTTGATC TGACTTCTCG GGATGGAGCC 
TGGTTCTTGG AGGAACTCTG CAGTATGAGC GGAAACGTCA CCTGCTTGGT TCAGTTACTG 
AAGCAGTGCC ACCTGGTGCC ACAGGACTTA GATATCCCGA ACCCCATGGA AGCGTCTGAG 
ACAGTTCACT TAGCCAATGG AGTGTATACC TCACTTCAGG AATTGAATTC GAATTTCCGG 
CAAATCATAT TTCCAGAAGC ACTTCGATGT TTAATGAAAG GGGAATACAC GTTAGAAAGT 
ATGCTGCATG AACTGGACGG TCTTATTGAG CAGACCACCG ATGGCGTTCC CCTGCAGACT 
CTAGTGGAAT CTCTTCAGGC CTACTTAAGA AACGCAGCTA TGGGACTGGA AGAAGAAACA 
CATGCTCATT ACATCGATGT TGCCAGACTA CTACATGCTC AGTACGGTGA ATTAATCCAA 
CCGAGAAATG GTTCAGTTGA TGAAACACCC AAAATGTCAG CTGGCCAGAT GCTTTTGGTA 
GCATTCGATG GCATGTTTGC TCAAGTTGAA ACTGCTTTCA GCTTATTAGT TGAAAAGTTG 
AACAAGATGG AAATTCCCAT AGCTTGGCGA AAGATTGACA TCATAAGGGA AGCCAGGAGT 
ACTCAAGTTA ATTTTTTTGA TGATGATAAT CACCGGCAGG TGCTAGAAGA GATTTTCTTT 
CTAAAAAGAC TACAGACTAT TAAGGAGTTC TTCAGGCTCT GTGGTACCTT TTCTAAAACA 
TTGTCAGGAT CAAGTTCACT TGAAGATCAG AATACTGTGA ATGGGCCTGT ACAGATTGTC 
AATGTGAAAA CCCTTTTTAG AAACTCTTGT TTCAGTGAAG ACCAAATGGC CAAACCTATC 
AAGGCATTCA CAGCTGACTT TGTGAGGCAG CTCTTGATAG GGCTACCCAA CCAAGCCCTC 
GGACTCACAC TGTGCAGTTT TATCAGTGCT CTGGGTGTAG ACATCATTGC TCAAGTAGAG 
GCAAAGGACT TTGGTGCCGA AAGCAAAGTT TCTGTTGATG ATCTCTGTAA GAAAGCGGTG 
GAACATAACA TCCAGATAGG GAAGTTCTCT CAGCTGGTTA TGAACAGGGC AACTGTGTTA 
GCAAGTTCTT ACGACACTGC CTGGAAGAAG CATGACTTGG TGCGAAGGCT AGAAACCAGT 
ATTTCTTCTT GTAAGACAAG CCTGCAGCGG GTTCAGCTGC ATATTGCCAT GTTTCAGTGG 
CAACATGAAG ATCTACTTAT CAATAGACCA CAAGCCATGT CAGTCACACC TCCCCCACGG 
TCTGCTATCC TAACCAGCAT GAAAAAGAAG CTGCATACCC TGAGCCAGAT TGAAACTTCT 
ATTGCAACAG TTCAGGAGAA GCTAGCTGCA CTTGAATCAA GTATTGAACA GCGACTCAAG 
TGGGCAGGTG GTGCCAACCC TGCATTGGCC CCTGTACTAC AAGATTTTGA AGCAACGATA 
GCTGAAAGAA GAAATCTTGT CCTTAAAGAG AGCCAAAGAG CAAGTCAGGT CACATTTCTC 
TGCAGCAATA TCATTCATTT TGAAAGTTTA CGAACAAGAA CTGCAGAAGC CTTAAACCTG 
GATGCGGCGT TATTTGAACT AATCAAGCGA TGTCAGCAGA TGTGTTCGTT TGCATCACAG 
TTTAACAGTT CAGTGTCTGA GTTAGAGCTT CGTTTATTAC AGAGAGTGGA CACTGGTCTT 
GAACATCCTA TTGGCAGCTC TGAATGGCTT TTGTCAGCAC ACAAACAGTT GACCCAGGAT 
ATGTCTACTC AGAGGGCAAT TCAGACAGAG AAAGAGCAGC AGATAGAAAC GGTCTGTGAA 
ACAATTCAGA ATCTGGTTGA TAATATAAAG ACTGTGCTCA CTGGTCATAA CCGACAGCTT 
GGAGATGTCA AACATCTCTT GAAAGCTATG GCTAAGGATG AAGAAGCTGC TCTGGCAGAT 
GGTGAAGATG TTCCCTATGA GAACAGTGTT AGGCAGTTTT TGGGTGAATA TAAATCATGG 
CAAGACAACA TTCAAACAGT TCTATTTACA TTAGTCCAGG CTATGGGTCA GGTTCGAAGT 
CAAGAACACG TTGAAATGCT CCAGGAAATC ACTCCCACCT TGAAAGAACT GAAAACACAA 
AGTCAGAGTA TCTATAATAA TTTAGTGAGT TTTGCATCAC CCTTAGTCAC CGATGCAACA 
AATGAATGTT CGAGTCCAAC GTCATCTGCT ACTTATCAGC CATCCTTCGC TGCAGCAGTC 
CGGAGTAACA CTGGCCAGAA GACTCAGCCT GATGTCATGT CACAGAATGC TAGAAAGCTG 
ATCCAAAAAA ATCTTGCTAC ATCAGCTGAT ACTCCACCAA GCACCGTTCC AGGAACTGGC 
AAGAGTGTTG CTTGTAGTCC TAAAAAGGCA GTCAGAGACC CTAAAACTGG GAAAGCGGTG 
CAAGAGAGAA ACTCCTATGC AGTGAGTGTG TGGAAGAGAG TGAAAGCCAA GTTAGAGGGC 
CGAGATGTTG ATCCGAATAG GAGGATGTCA GTTGCTGAAC AGGTTGACTA TGTCATTAAG 
GAAGCAACTA ATCTAGATAA CTTGGCTCAG CTGTATGAAG GTTGGACAGC CTGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG1 ( XM_017023067.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878556.1 Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X9, mRNA.

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CloneID	OHu65358
Clone ID Related Accession (Same CDS sequence)	XM_011545770.1 , XM_017023067.1
Accession Version	XM_017023067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase SMG1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCGC TATCTCCAAC TGTCTTTGCA CTTCTGAGTA AGAATCTGAT GATTGTGCAC 
AGTGACCTGG CTGTTCACTT CCCTGCCATT CAGTATGCTG TGCTCTACAC ATTGTATTCT 
CATTGTACCA GGCATGATCA CTTTATCTCT AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG 
TTTGATGGAG CTGTGATTAG CACTGTAACT ACGGCTACAA AGAAACATTT CTCAATTATA 
TTAAATCTTC TGGGAATATT ACTTAAGAAA GATAACCTTA ACCAGGACAC GAGGAAACTG 
TTAATGACTT GGGCTTTGGA AGCAGCTGTT TTAATGAAGA AGTCTGAAAC ATACGCACCT 
TTATTCTCTC TTCCGTCTTT CCATAAATTT TGCAAAGGCC TTTTAGCCAA CACTCTCGTT 
GAAGATGTGA ATATCTGTCT GCAGGCATGC AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG 
CCAGATGATC TTTTACAGAG ATGTGTCGAT GTTTGCCGTG TTCAACTAGT GCACAGTGGA 
ACTCGTATTC GACAAGCATT TGGAAAACTG TTGAAATCAA TTCCTTTAGA TGTTGTCCTA 
AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT 
AAAGCACCAA GTAATACATT CCACCCCCAA GATTTCTCTG ATGTTATTAG TTTTATTTTG 
TATGGGAACT CTCATAGAAC AGGGAAGGAC AATTGGTTGG AAAGACTGTT CTATAGCTGC 
CAGAGACTGG ATAAGCGTGA CCAGTCAACA ATTCCACGCA ATCTCCTGAA GACAGATGCT 
GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT GCACAATTCA CTGTTCTTTC TAAGCTGAGA 
ACCCCACTGG GCAGAGCTCA AGACACCTTC CAGACAATTG AAGGTATCAT TCGAAGTCTC 
GCAGCTCACA CATTAAACCC TGATCAGGAT GTTAGTCAGT GGACAACTGC AGACAATGAT 
GAAGGCCATG GTAACAACCA ACTTAGACTT GTTCTTCTTC TGCAGTATCT GGAAAATCTG 
GAGAAATTAA TGTATAATGC ATACGAGGGA TGTGCTAATG CATTAACTTC ACCTCCCAAG 
GTCATTAGAA CTTTTTTCTA TACCAATCGC CAAACTTGTC AGGACTGGCT AACGCGGATT 
CGACTCTCCA TCATGAGGGT AGGATTGTTG GCAGGCCAGC CTGCAGTGAC AGTGAGACAT 
GGCTTTGACT TGCTTACAGA GATGAAAACA ACCAGCCTAT CTCAGGGGAA TGAATTGGAA 
GTAACCATTA TGATGGTGGT AGAAGCATTA TGTGAACTTC ATTGTCCTGA AGCTATACAG 
GGAATTGCTG TCTGGTCATC ATCTATTGTT GGAAAAAATC TTCTGTGGAT TAACTCAGTG 
GCTCAACAGG CTGAAGGGAG GTTTGAAAAG GCCTCTGTGG AGTACCAGGA ACACCTGTGT 
GCCATGACAG GTGTTGATTG CTGCATCTCC AGCTTTGACA AATCGGTGCT CACCTTAGCC 
AATGCTGGGC GTAACAGTGC CAGCCCGAAA CATTCTCTGA ATGGTGAATC CAGAAAAACT 
GTGCTGTCCA AACCGACTGA CTCTTCCCCT GAGGTTATAA ATTATTTAGG AAATAAAGCA 
TGTGAGTGCT ACATCTCAAT TGCCGATTGG GCTGCTGTGC AGGAATGGCA GAACGCTATC 
CATGACTTGA AAAAGAGTAC CAGTAGCACT TCCCTCAACC TGAAAGCTGA CTTCAACTAT 
ATAAAATCAT TAAGCAGCTT TGAGTCTGGA AAATTTGTTG AATGTACCGA GCAGTTAGAA 
TTGTTACCAG GAGAAAATAT CAATCTACTT GCTGGAGGAT CAAAAGAAAA AATAGACATG 
AAAAAACTGC TTCCTAACAT GTTAAGTCCG GATCCGAGGG AACTTCAGAA ATCCATTGAA 
GTTCAATTGT TAAGAAGTTC TGTTTGTTTG GCAACTGCTT TAAACCCGAT AGAACAAGAT 
CAGAAGTGGC AGTCTATAAC TGAAAATGTG GTAAAGTACT TGAAGCAAAC ATCCCGCATC 
GCTATTGGAC CTCTGAGACT TTCTACTTTA ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT 
ACCTTGCAGC TGTATTGCTC ATCTGCTTTG GAGAACACAG TTTCTAACAG ACTTTCAACA 
GAGGACTGTC TTATTCCACT CTTCAGTGAA GCTTTACGTT CATGTAAACA GCATGACGTG 
AGGCCATGGA TGCAGGCATT AAGGTATACT ATGTACCAGA ATCAGTTGTT GGAGAAAATT 
AAAGAACAAA CAGTCCCAAT TAGAAGCCAT CTCATGGAAT TAGGTCTAAC AGCAGCAAAA 
TTTGCTAGAA AACGAGGGAA TGTGTCCCTT GCAACAAGAC TGCTGGCACA GTGCAGTGAA 
GTTCAGCTGG GAAAGACCAC CACTGCACAG GATTTAGTCC AACATTTTAA AAAACTATCA 
ACCCAAGGTC AAGTGGATGA AAAATGGGGG CCCGAACTTG ATATTGAAAA AACCAAATTG 
CTTTATACAG CAGGCCAGTC AACACATGCA ATGGAAATGT TGAGTTCTTG TGCCATATCT 
TTCTGCAAGT CTGTGAAAGC TGAATATGCA GTTGCTAAAT CAATTCTGAC ACTGGCTAAA 
TGGATCCAGG CAGAATGGAA AGAGATTTCA GGACAGCTGA AACAGGTTTA CAGAGCTCAG 
CACCAACAGA ACTTCACAGG TCTTTCTACT TTGTCTAAAA ACATACTCAC TCTAATAGAA 
CTGCCATCTG TTAATACGAT GGAAGAAGAG TATCCTCGGA TCGAGAGTGA ATCTACAGTG 
CATATTGGAG TTGGAGAACC TGACTTCATT TTGGGACAGT TGTATCACCT GTCTTCAGTA 
CAGGCACCTG AAGTAGCCAA ATCTTGGGCA GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC 
AGAAAGGTGG TTGACAATGC CAGTCAGGGA GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA 
TCTGAAGTTC AGAATCTACT TCCAGACACT ATAACTGAGG AAGAGAAAGA GAGAATATAT 
GGTATTCTTG GACAGGCTGT GTGTCGGCCG GCGGGGATTC AGGATGAAGA TATAACACTT 
CAGATAACTG AGAGTGAAGA CAACGAAGAA GATGACATGG TTGATGTTAT CTGGCGTCAG 
TTGATATCAA GCTGCCCATG GCTTTCAGAA CTTGATGAAA GTGCAACTGA AGGAGTTATT 
AAAGTGTGGA GGAAAGTTGT AGATAGAATA TTCAGCCTGT ACAAACTCTC TTGCAGTGCA 
TACTTTACTT TCCTTAAACT CAACGCTGGT CAAATTCCTT TAGATGAGGA TGACCCTAGG 
CTGCATTTAA GTCACAGAGT GGAACAGAGC ACTGATGACA TGATTGTGAT GGCCACATTG 
CGCCTGCTGC GGTTGCTCGT GAAGCATGCT GGTGAGCTTC GGCAGTATCT GGAGCACGGC 
TTGGAGACAA CACCCACTGC ACCATGGAGA GGAATTATTC CGCAACTTTT CTCACGCTTA 
AACCACCCTG AAGTGTATGT GCGCCAAAGT ATTTGTAACC TTCTCTGCCG TGTGGCTCAA 
GATTCCCCAC ATCTCATATT GTATCCTGCA ATAGTGGGTA CCATATCGCT TAGTAGTGAA 
TCCCAGGCTT CAGGAAATAA ATTTTCCACT GCAATTCCAA CTTTACTTGG CAATATTCAA 
GGAGAAGAAT TGCTGGTTTC TGAATGTGAG GGAGGAAGTC CTCCTGCATC TCAGGATAGC 
AATAAGGATG AACCTAAAAG TGGATTAAAT GAAGACCAAG CCATGATGCA GGATTGTTAC 
AGCAAAATTG TAGATAAGCT GTCCTCTGCA AACCCCACCA TGGTATTACA GGTTCAGATG 
CTCGTGGCTG AACTGCGCAG GGTCACTGTG CTCTGGGATG AGCTCTGGCT GGGAGTTTTG 
CTGCAACAAC ACATGTATGT CCTGAGACGA ATTCAGCAGC TTGAAGATGA GGTGAAGAGA 
GTCCAGAACA ACAACACCTT ACGCAAAGAA GAGAAAATTG CAATCATGAG GGAGAAGCAC 
ACAGCTTTGA TGAAGCCCAT CGTATTTGCT TTGGAGCATG TGAGGAGTAT CACAGCGGCT 
CCTGCAGAAA CACCTCATGA AAAATGGTTT CAGGATAACT ATGGTGATGC CATTGAAAAT 
GCCCTAGAAA AACTGAAGAC TCCATTGAAC CCTGCAAAGC CTGGGAGCAG CTGGATTCCA 
TTTAAAGAGA TAATGCTAAG TTTGCAACAG AGAGCACAGA AACGTGCAAG TTACATCTTG 
CGTCTTGAAG AAATCAGTCC ATGGTTGGCT GCCATGACTA ACACTGAAAT TGCTCTTCCT 
GGGGAAGTCT CAGCCAGAGA CACTGTCACA ATCCATAGTG TGGGCGGAAC CATCACAATC 
TTACCGACTA AAACCAAGCC AAAGAAACTT CTCTTTCTTG GATCAGATGG GAAGAGCTAT 
CCTTATCTTT TCAAAGGACT GGAGGATTTA CATCTGGATG AGAGAATAAT GCAGTTCCTA 
TCTATTGTGA ATACCATGTT TGCTACAATT AATCGCCAAG AAACACCCCG GTTCCATGCT 
CGACACTATT CTGTAACACC ACTAGGAACA AGATCAGGAC TAATCCAGTG GGTAGATGGA 
GCCACACCCT TATTTGGTCT TTACAAACGA TGGCAACAAC GGGAAGCTGC CTTACAAGCA 
CAAAAGGCCC AAGATTCCTA CCAAACTCCT CAGAATCCTG GAATTGTACC CCGTCCTAGT 
GAACTTTATT ACAGTAAAAT TGGCCCTGCT TTGAAAACAG TTGGGCTTAG CCTGGATGTG 
TCCCGTCGGG ATTGGCCTCT TCATGTAATG AAGGCAGTAT TGGAAGAGTT AATGGAGGCC 
ACACCCCCGA ATCTCCTTGC CAAAGAGCTC TGGTCATCTT GCACAACACC TGATGAATGG 
TGGAGAGTTA CGCAGTCTTA TGCAAGATCT ACTGCAGTCA TGTCTATGGT TGGATACATA 
ATTGGCCTTG GAGACAGACA TCTGGATAAT GTTCTTATAG ATATGACGAC TGGAGAAGTT 
GTTCACATAG ATTACAATGT TTGCTTTGAA AAAGGTAATA TAAAAGTAGA CTTTACATTT 
ATGAGGGCCA AATGTTTTTT GGTGAAAGGG CAAAAATATA ATGATTTGTC TTTTCAAGGT 
AAAAGCCTTA GAGTTCCTGA GAAAGTACCT TTTCGAATGA CACAAAACAT TGAAACAGCA 
CTGGGTGTAA CTGGAGTAGA AGGTGTATTT AGGCTTTCAT GTGAGCAGGT TTTACACATT 
ATGCGGCGTG GCAGAGAGAC CCTGCTGACG CTGCTGGAGG CCTTTGTGTA CGACCCTCTG 
GTGGACTGGA CAGCAGGAGG CGAGGCTGGG TTTGCTGGTG CTGTCTATGG TGGAGGTGGC 
CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA GAGATGGAGC GAGAGATCAC CCGCAGCCTG 
TTTTCTTCTA GAGTAGCTGA GATTAAGGTG AACTGGTTTA AGAATAGAGA TGAGATGCTG 
GTTGTGCTTC CCAAGTTGGA CGGTAGCTTA GATGAATACC TAAGCTTGCA AGAGCAACTG 
ACAGATGTGG AAAAACTGCA GGGCAAACTA CTGGAGGAAA TAGAGTTTCT AGAAGGAGCT 
GAAGGGGTGG ATCATCCTTC TCATACTCTG CAACACAGGT ATTCTGAGCA CACCCAACTA 
CAGACTCAGC AAAGAGCTGT TCAGGAAGCA ATCCAGGTGA AGCTGAATGA ATTTGAACAA 
TGGATAACAC ATTATCAGGC TGCATTCAAT AATTTAGAAG CAACACAGCT TGCAAGCTTG 
CTTCAAGAGA TAAGCACACA AATGGACCTT GGTCCTCCAA GTTACGTGCC AGCAACAGCC 
TTTCTGCAGA ATGCTGGTCA GGCCCACTTG ATTAGCCAGT GCGAGCAGCT GGAGGGGGAG 
GTTGGTGCTC TCCTGCAGCA GAGGCGCTCC GTGCTCCGTG GCTGTCTGGA GCAACTGCAT 
CACTATGCAA CCGTGGCCCT GCAGTATCCG AAGGCCATAT TTCAGAAACA TCGAATTGAA 
CAGTGGAAGA CCTGGATGGA AGAGCTCATC TGTAACACCA CAGTAGAGCG TTGTCAAGAG 
CTCTATAGGA AATATGAAAT GCAATATGCT CCCCAGCCAC CCCCAACAGT GTGTCAGTTC 
ATCACTGCCA CTGAAATGAC CCTGCAGCGA TACGCAGCAG ACATCAACAG CAGACTTATT 
AGACAAGTGG AACGCTTGAA ACAGGAAGCT GTCACTGTGC CAGTTTGTGA AGATCAGTTG 
AAAGAAATTG AACGTTGCAT TAAAGTTTTC CTTCATGAGA ATGGAGAAGA AGGATCTTTG 
AGTCTAGCAA GTGTTATTAT TTCTGCCCTT TGTACCCTTA CAAGGCGTAA CCTGATGATG 
GAAGGTGCAG CGTCAAGTGC TGGAGAACAG CTGGTTGATC TGACTTCTCG GGATGGAGCC 
TGGTTCTTGG AGGAACTCTG CAGTATGAGC GGAAACGTCA CCTGCTTGGT TCAGTTACTG 
AAGCAGTGCC ACCTGGTGCC ACAGGACTTA GATATCCCGA ACCCCATGGA AGCGTCTGAG 
ACAGTTCACT TAGCCAATGG AGTGTATACC TCACTTCAGG AATTGAATTC GAATTTCCGG 
CAAATCATAT TTCCAGAAGC ACTTCGATGT TTAATGAAAG GGGAATACAC GTTAGAAAGT 
ATGCTGCATG AACTGGACGG TCTTATTGAG CAGACCACCG ATGGCGTTCC CCTGCAGACT 
CTAGTGGAAT CTCTTCAGGC CTACTTAAGA AACGCAGCTA TGGGACTGGA AGAAGAAACA 
CATGCTCATT ACATCGATGT TGCCAGACTA CTACATGCTC AGTACGGTGA ATTAATCCAA 
CCGAGAAATG GTTCAGTTGA TGAAACACCC AAAATGTCAG CTGGCCAGAT GCTTTTGGTA 
GCATTCGATG GCATGTTTGC TCAAGTTGAA ACTGCTTTCA GCTTATTAGT TGAAAAGTTG 
AACAAGATGG AAATTCCCAT AGCTTGGCGA AAGATTGACA TCATAAGGGA AGCCAGGAGT 
ACTCAAGTTA ATTTTTTTGA TGATGATAAT CACCGGCAGG TGCTAGAAGA GATTTTCTTT 
CTAAAAAGAC TACAGACTAT TAAGGAGTTC TTCAGGCTCT GTGGTACCTT TTCTAAAACA 
TTGTCAGGAT CAAGTTCACT TGAAGATCAG AATACTGTGA ATGGGCCTGT ACAGATTGTC 
AATGTGAAAA CCCTTTTTAG AAACTCTTGT TTCAGTGAAG ACCAAATGGC CAAACCTATC 
AAGGCATTCA CAGCTGACTT TGTGAGGCAG CTCTTGATAG GGCTACCCAA CCAAGCCCTC 
GGACTCACAC TGTGCAGTTT TATCAGTGCT CTGGGTGTAG ACATCATTGC TCAAGTAGAG 
GCAAAGGACT TTGGTGCCGA AAGCAAAGTT TCTGTTGATG ATCTCTGTAA GAAAGCGGTG 
GAACATAACA TCCAGATAGG GAAGTTCTCT CAGCTGGTTA TGAACAGGGC AACTGTGTTA 
GCAAGTTCTT ACGACACTGC CTGGAAGAAG CATGACTTGG TGCGAAGGCT AGAAACCAGT 
ATTTCTTCTT GTAAGACAAG CCTGCAGCGG GTTCAGCTGC ATATTGCCAT GTTTCAGTGG 
CAACATGAAG ATCTACTTAT CAATAGACCA CAAGCCATGT CAGTCACACC TCCCCCACGG 
TCTGCTATCC TAACCAGCAT GAAAAAGAAG CTGCATACCC TGAGCCAGAT TGAAACTTCT 
ATTGCAACAG TTCAGGAGAA GCTAGCTGCA CTTGAATCAA GTATTGAACA GCGACTCAAG 
TGGGCAGGTG GTGCCAACCC TGCATTGGCC CCTGTACTAC AAGATTTTGA AGCAACGATA 
GCTGAAAGAA GAAATCTTGT CCTTAAAGAG AGCCAAAGAG CAAGTCAGGT CACATTTCTC 
TGCAGCAATA TCATTCATTT TGAAAGTTTA CGAACAAGAA CTGCAGAAGC CTTAAACCTG 
GATGCGGCGT TATTTGAACT AATCAAGCGA TGTCAGCAGA TGTGTTCGTT TGCATCACAG 
TTTAACAGTT CAGTGTCTGA GTTAGAGCTT CGTTTATTAC AGAGAGTGGA CACTGGTCTT 
GAACATCCTA TTGGCAGCTC TGAATGGCTT TTGTCAGCAC ACAAACAGTT GACCCAGGAT 
ATGTCTACTC AGAGGGCAAT TCAGACAGAG AAAGAGCAGC AGATAGAAAC GGTCTGTGAA 
ACAATTCAGA ATCTGGTTGA TAATATAAAG ACTGTGCTCA CTGGTCATAA CCGACAGCTT 
GGAGATGTCA AACATCTCTT GAAAGCTATG GCTAAGGATG AAGAAGCTGC TCTGGCAGAT 
GGTGAAGATG TTCCCTATGA GAACAGTGTT AGGCAGTTTT TGGGTGAATA TAAATCATGG 
CAAGACAACA TTCAAACAGT TCTATTTACA TTAGTCCAGG CTATGGGTCA GGTTCGAAGT 
CAAGAACACG TTGAAATGCT CCAGGAAATC ACTCCCACCT TGAAAGAACT GAAAACACAA 
AGTCAGAGTA TCTATAATAA TTTAGTGAGT TTTGCATCAC CCTTAGTCAC CGATGCAACA 
AATGAATGTT CGAGTCCAAC GTCATCTGCT ACTTATCAGC CATCCTTCGC TGCAGCAGTC 
CGGAGTAACA CTGGCCAGAA GACTCAGCCT GATGTCATGT CACAGAATGC TAGAAAGCTG 
ATCCAAAAAA ATCTTGCTAC ATCAGCTGAT ACTCCACCAA GCACCGTTCC AGGAACTGGC 
AAGAGTGTTG CTTGTAGTCC TAAAAAGGCA GTCAGAGACC CTAAAACTGG GAAAGCGGTG 
CAAGAGAGAA ACTCCTATGC AGTGAGTGTG TGGAAGAGAG TGAAAGCCAA GTTAGAGGGC 
CGAGATGTTG ATCCGAATAG GAGGATGTCA GTTGCTGAAC AGGTTGACTA TGTCATTAAG 
GAAGCAACTA ATCTAGATAA CTTGGCTCAG CTGTATGAAG GTTGGACAGC CTGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878556.1
CDS	127..9306
Translation	MWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGNIKVDFTFMRAKCFLVKGQKYNDLSFQGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_017023067.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG1 nonsense mediated mRNA decay associated PI3K related kinase (SMG1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023067.1

ORF Insert Sequence:

ATGTGGGCGC TATCTCCAAC TGTCTTTGCA CTTCTGAGTA AGAATCTGAT GATTGTGCAC 
AGTGACCTGG CTGTTCACTT CCCTGCCATT CAGTATGCTG TGCTCTACAC ATTGTATTCT 
CATTGTACCA GGCATGATCA CTTTATCTCT AGTAGCCTCA GTTCTTCCTC TCCTTCTTTG 
TTTGATGGAG CTGTGATTAG CACTGTAACT ACGGCTACAA AGAAACATTT CTCAATTATA 
TTAAATCTTC TGGGAATATT ACTTAAGAAA GATAACCTTA ACCAGGACAC GAGGAAACTG 
TTAATGACTT GGGCTTTGGA AGCAGCTGTT TTAATGAAGA AGTCTGAAAC ATACGCACCT 
TTATTCTCTC TTCCGTCTTT CCATAAATTT TGCAAAGGCC TTTTAGCCAA CACTCTCGTT 
GAAGATGTGA ATATCTGTCT GCAGGCATGC AGCAGTCTAC ATGCTCTGTC CTCTTCCTTG 
CCAGATGATC TTTTACAGAG ATGTGTCGAT GTTTGCCGTG TTCAACTAGT GCACAGTGGA 
ACTCGTATTC GACAAGCATT TGGAAAACTG TTGAAATCAA TTCCTTTAGA TGTTGTCCTA 
AGCAATAACA ATCACACAGA AATTCAAGAA ATTTCTTTAG CATTAAGAAG TCACATGAGT 
AAAGCACCAA GTAATACATT CCACCCCCAA GATTTCTCTG ATGTTATTAG TTTTATTTTG 
TATGGGAACT CTCATAGAAC AGGGAAGGAC AATTGGTTGG AAAGACTGTT CTATAGCTGC 
CAGAGACTGG ATAAGCGTGA CCAGTCAACA ATTCCACGCA ATCTCCTGAA GACAGATGCT 
GTCCTTTGGC AGTGGGCCAT ATGGGAAGCT GCACAATTCA CTGTTCTTTC TAAGCTGAGA 
ACCCCACTGG GCAGAGCTCA AGACACCTTC CAGACAATTG AAGGTATCAT TCGAAGTCTC 
GCAGCTCACA CATTAAACCC TGATCAGGAT GTTAGTCAGT GGACAACTGC AGACAATGAT 
GAAGGCCATG GTAACAACCA ACTTAGACTT GTTCTTCTTC TGCAGTATCT GGAAAATCTG 
GAGAAATTAA TGTATAATGC ATACGAGGGA TGTGCTAATG CATTAACTTC ACCTCCCAAG 
GTCATTAGAA CTTTTTTCTA TACCAATCGC CAAACTTGTC AGGACTGGCT AACGCGGATT 
CGACTCTCCA TCATGAGGGT AGGATTGTTG GCAGGCCAGC CTGCAGTGAC AGTGAGACAT 
GGCTTTGACT TGCTTACAGA GATGAAAACA ACCAGCCTAT CTCAGGGGAA TGAATTGGAA 
GTAACCATTA TGATGGTGGT AGAAGCATTA TGTGAACTTC ATTGTCCTGA AGCTATACAG 
GGAATTGCTG TCTGGTCATC ATCTATTGTT GGAAAAAATC TTCTGTGGAT TAACTCAGTG 
GCTCAACAGG CTGAAGGGAG GTTTGAAAAG GCCTCTGTGG AGTACCAGGA ACACCTGTGT 
GCCATGACAG GTGTTGATTG CTGCATCTCC AGCTTTGACA AATCGGTGCT CACCTTAGCC 
AATGCTGGGC GTAACAGTGC CAGCCCGAAA CATTCTCTGA ATGGTGAATC CAGAAAAACT 
GTGCTGTCCA AACCGACTGA CTCTTCCCCT GAGGTTATAA ATTATTTAGG AAATAAAGCA 
TGTGAGTGCT ACATCTCAAT TGCCGATTGG GCTGCTGTGC AGGAATGGCA GAACGCTATC 
CATGACTTGA AAAAGAGTAC CAGTAGCACT TCCCTCAACC TGAAAGCTGA CTTCAACTAT 
ATAAAATCAT TAAGCAGCTT TGAGTCTGGA AAATTTGTTG AATGTACCGA GCAGTTAGAA 
TTGTTACCAG GAGAAAATAT CAATCTACTT GCTGGAGGAT CAAAAGAAAA AATAGACATG 
AAAAAACTGC TTCCTAACAT GTTAAGTCCG GATCCGAGGG AACTTCAGAA ATCCATTGAA 
GTTCAATTGT TAAGAAGTTC TGTTTGTTTG GCAACTGCTT TAAACCCGAT AGAACAAGAT 
CAGAAGTGGC AGTCTATAAC TGAAAATGTG GTAAAGTACT TGAAGCAAAC ATCCCGCATC 
GCTATTGGAC CTCTGAGACT TTCTACTTTA ACAGTTTCAC AGTCTTTGCC AGTTCTAAGT 
ACCTTGCAGC TGTATTGCTC ATCTGCTTTG GAGAACACAG TTTCTAACAG ACTTTCAACA 
GAGGACTGTC TTATTCCACT CTTCAGTGAA GCTTTACGTT CATGTAAACA GCATGACGTG 
AGGCCATGGA TGCAGGCATT AAGGTATACT ATGTACCAGA ATCAGTTGTT GGAGAAAATT 
AAAGAACAAA CAGTCCCAAT TAGAAGCCAT CTCATGGAAT TAGGTCTAAC AGCAGCAAAA 
TTTGCTAGAA AACGAGGGAA TGTGTCCCTT GCAACAAGAC TGCTGGCACA GTGCAGTGAA 
GTTCAGCTGG GAAAGACCAC CACTGCACAG GATTTAGTCC AACATTTTAA AAAACTATCA 
ACCCAAGGTC AAGTGGATGA AAAATGGGGG CCCGAACTTG ATATTGAAAA AACCAAATTG 
CTTTATACAG CAGGCCAGTC AACACATGCA ATGGAAATGT TGAGTTCTTG TGCCATATCT 
TTCTGCAAGT CTGTGAAAGC TGAATATGCA GTTGCTAAAT CAATTCTGAC ACTGGCTAAA 
TGGATCCAGG CAGAATGGAA AGAGATTTCA GGACAGCTGA AACAGGTTTA CAGAGCTCAG 
CACCAACAGA ACTTCACAGG TCTTTCTACT TTGTCTAAAA ACATACTCAC TCTAATAGAA 
CTGCCATCTG TTAATACGAT GGAAGAAGAG TATCCTCGGA TCGAGAGTGA ATCTACAGTG 
CATATTGGAG TTGGAGAACC TGACTTCATT TTGGGACAGT TGTATCACCT GTCTTCAGTA 
CAGGCACCTG AAGTAGCCAA ATCTTGGGCA GCGTTGGCCA GCTGGGCTTA TAGGTGGGGC 
AGAAAGGTGG TTGACAATGC CAGTCAGGGA GAAGGTGTTC GTCTGCTGCC TAGAGAAAAA 
TCTGAAGTTC AGAATCTACT TCCAGACACT ATAACTGAGG AAGAGAAAGA GAGAATATAT 
GGTATTCTTG GACAGGCTGT GTGTCGGCCG GCGGGGATTC AGGATGAAGA TATAACACTT 
CAGATAACTG AGAGTGAAGA CAACGAAGAA GATGACATGG TTGATGTTAT CTGGCGTCAG 
TTGATATCAA GCTGCCCATG GCTTTCAGAA CTTGATGAAA GTGCAACTGA AGGAGTTATT 
AAAGTGTGGA GGAAAGTTGT AGATAGAATA TTCAGCCTGT ACAAACTCTC TTGCAGTGCA 
TACTTTACTT TCCTTAAACT CAACGCTGGT CAAATTCCTT TAGATGAGGA TGACCCTAGG 
CTGCATTTAA GTCACAGAGT GGAACAGAGC ACTGATGACA TGATTGTGAT GGCCACATTG 
CGCCTGCTGC GGTTGCTCGT GAAGCATGCT GGTGAGCTTC GGCAGTATCT GGAGCACGGC 
TTGGAGACAA CACCCACTGC ACCATGGAGA GGAATTATTC CGCAACTTTT CTCACGCTTA 
AACCACCCTG AAGTGTATGT GCGCCAAAGT ATTTGTAACC TTCTCTGCCG TGTGGCTCAA 
GATTCCCCAC ATCTCATATT GTATCCTGCA ATAGTGGGTA CCATATCGCT TAGTAGTGAA 
TCCCAGGCTT CAGGAAATAA ATTTTCCACT GCAATTCCAA CTTTACTTGG CAATATTCAA 
GGAGAAGAAT TGCTGGTTTC TGAATGTGAG GGAGGAAGTC CTCCTGCATC TCAGGATAGC 
AATAAGGATG AACCTAAAAG TGGATTAAAT GAAGACCAAG CCATGATGCA GGATTGTTAC 
AGCAAAATTG TAGATAAGCT GTCCTCTGCA AACCCCACCA TGGTATTACA GGTTCAGATG 
CTCGTGGCTG AACTGCGCAG GGTCACTGTG CTCTGGGATG AGCTCTGGCT GGGAGTTTTG 
CTGCAACAAC ACATGTATGT CCTGAGACGA ATTCAGCAGC TTGAAGATGA GGTGAAGAGA 
GTCCAGAACA ACAACACCTT ACGCAAAGAA GAGAAAATTG CAATCATGAG GGAGAAGCAC 
ACAGCTTTGA TGAAGCCCAT CGTATTTGCT TTGGAGCATG TGAGGAGTAT CACAGCGGCT 
CCTGCAGAAA CACCTCATGA AAAATGGTTT CAGGATAACT ATGGTGATGC CATTGAAAAT 
GCCCTAGAAA AACTGAAGAC TCCATTGAAC CCTGCAAAGC CTGGGAGCAG CTGGATTCCA 
TTTAAAGAGA TAATGCTAAG TTTGCAACAG AGAGCACAGA AACGTGCAAG TTACATCTTG 
CGTCTTGAAG AAATCAGTCC ATGGTTGGCT GCCATGACTA ACACTGAAAT TGCTCTTCCT 
GGGGAAGTCT CAGCCAGAGA CACTGTCACA ATCCATAGTG TGGGCGGAAC CATCACAATC 
TTACCGACTA AAACCAAGCC AAAGAAACTT CTCTTTCTTG GATCAGATGG GAAGAGCTAT 
CCTTATCTTT TCAAAGGACT GGAGGATTTA CATCTGGATG AGAGAATAAT GCAGTTCCTA 
TCTATTGTGA ATACCATGTT TGCTACAATT AATCGCCAAG AAACACCCCG GTTCCATGCT 
CGACACTATT CTGTAACACC ACTAGGAACA AGATCAGGAC TAATCCAGTG GGTAGATGGA 
GCCACACCCT TATTTGGTCT TTACAAACGA TGGCAACAAC GGGAAGCTGC CTTACAAGCA 
CAAAAGGCCC AAGATTCCTA CCAAACTCCT CAGAATCCTG GAATTGTACC CCGTCCTAGT 
GAACTTTATT ACAGTAAAAT TGGCCCTGCT TTGAAAACAG TTGGGCTTAG CCTGGATGTG 
TCCCGTCGGG ATTGGCCTCT TCATGTAATG AAGGCAGTAT TGGAAGAGTT AATGGAGGCC 
ACACCCCCGA ATCTCCTTGC CAAAGAGCTC TGGTCATCTT GCACAACACC TGATGAATGG 
TGGAGAGTTA CGCAGTCTTA TGCAAGATCT ACTGCAGTCA TGTCTATGGT TGGATACATA 
ATTGGCCTTG GAGACAGACA TCTGGATAAT GTTCTTATAG ATATGACGAC TGGAGAAGTT 
GTTCACATAG ATTACAATGT TTGCTTTGAA AAAGGTAATA TAAAAGTAGA CTTTACATTT 
ATGAGGGCCA AATGTTTTTT GGTGAAAGGG CAAAAATATA ATGATTTGTC TTTTCAAGGT 
AAAAGCCTTA GAGTTCCTGA GAAAGTACCT TTTCGAATGA CACAAAACAT TGAAACAGCA 
CTGGGTGTAA CTGGAGTAGA AGGTGTATTT AGGCTTTCAT GTGAGCAGGT TTTACACATT 
ATGCGGCGTG GCAGAGAGAC CCTGCTGACG CTGCTGGAGG CCTTTGTGTA CGACCCTCTG 
GTGGACTGGA CAGCAGGAGG CGAGGCTGGG TTTGCTGGTG CTGTCTATGG TGGAGGTGGC 
CAGCAGGCCG AGAGCAAGCA GAGCAAGAGA GAGATGGAGC GAGAGATCAC CCGCAGCCTG 
TTTTCTTCTA GAGTAGCTGA GATTAAGGTG AACTGGTTTA AGAATAGAGA TGAGATGCTG 
GTTGTGCTTC CCAAGTTGGA CGGTAGCTTA GATGAATACC TAAGCTTGCA AGAGCAACTG 
ACAGATGTGG AAAAACTGCA GGGCAAACTA CTGGAGGAAA TAGAGTTTCT AGAAGGAGCT 
GAAGGGGTGG ATCATCCTTC TCATACTCTG CAACACAGGT ATTCTGAGCA CACCCAACTA 
CAGACTCAGC AAAGAGCTGT TCAGGAAGCA ATCCAGGTGA AGCTGAATGA ATTTGAACAA 
TGGATAACAC ATTATCAGGC TGCATTCAAT AATTTAGAAG CAACACAGCT TGCAAGCTTG 
CTTCAAGAGA TAAGCACACA AATGGACCTT GGTCCTCCAA GTTACGTGCC AGCAACAGCC 
TTTCTGCAGA ATGCTGGTCA GGCCCACTTG ATTAGCCAGT GCGAGCAGCT GGAGGGGGAG 
GTTGGTGCTC TCCTGCAGCA GAGGCGCTCC GTGCTCCGTG GCTGTCTGGA GCAACTGCAT 
CACTATGCAA CCGTGGCCCT GCAGTATCCG AAGGCCATAT TTCAGAAACA TCGAATTGAA 
CAGTGGAAGA CCTGGATGGA AGAGCTCATC TGTAACACCA CAGTAGAGCG TTGTCAAGAG 
CTCTATAGGA AATATGAAAT GCAATATGCT CCCCAGCCAC CCCCAACAGT GTGTCAGTTC 
ATCACTGCCA CTGAAATGAC CCTGCAGCGA TACGCAGCAG ACATCAACAG CAGACTTATT 
AGACAAGTGG AACGCTTGAA ACAGGAAGCT GTCACTGTGC CAGTTTGTGA AGATCAGTTG 
AAAGAAATTG AACGTTGCAT TAAAGTTTTC CTTCATGAGA ATGGAGAAGA AGGATCTTTG 
AGTCTAGCAA GTGTTATTAT TTCTGCCCTT TGTACCCTTA CAAGGCGTAA CCTGATGATG 
GAAGGTGCAG CGTCAAGTGC TGGAGAACAG CTGGTTGATC TGACTTCTCG GGATGGAGCC 
TGGTTCTTGG AGGAACTCTG CAGTATGAGC GGAAACGTCA CCTGCTTGGT TCAGTTACTG 
AAGCAGTGCC ACCTGGTGCC ACAGGACTTA GATATCCCGA ACCCCATGGA AGCGTCTGAG 
ACAGTTCACT TAGCCAATGG AGTGTATACC TCACTTCAGG AATTGAATTC GAATTTCCGG 
CAAATCATAT TTCCAGAAGC ACTTCGATGT TTAATGAAAG GGGAATACAC GTTAGAAAGT 
ATGCTGCATG AACTGGACGG TCTTATTGAG CAGACCACCG ATGGCGTTCC CCTGCAGACT 
CTAGTGGAAT CTCTTCAGGC CTACTTAAGA AACGCAGCTA TGGGACTGGA AGAAGAAACA 
CATGCTCATT ACATCGATGT TGCCAGACTA CTACATGCTC AGTACGGTGA ATTAATCCAA 
CCGAGAAATG GTTCAGTTGA TGAAACACCC AAAATGTCAG CTGGCCAGAT GCTTTTGGTA 
GCATTCGATG GCATGTTTGC TCAAGTTGAA ACTGCTTTCA GCTTATTAGT TGAAAAGTTG 
AACAAGATGG AAATTCCCAT AGCTTGGCGA AAGATTGACA TCATAAGGGA AGCCAGGAGT 
ACTCAAGTTA ATTTTTTTGA TGATGATAAT CACCGGCAGG TGCTAGAAGA GATTTTCTTT 
CTAAAAAGAC TACAGACTAT TAAGGAGTTC TTCAGGCTCT GTGGTACCTT TTCTAAAACA 
TTGTCAGGAT CAAGTTCACT TGAAGATCAG AATACTGTGA ATGGGCCTGT ACAGATTGTC 
AATGTGAAAA CCCTTTTTAG AAACTCTTGT TTCAGTGAAG ACCAAATGGC CAAACCTATC 
AAGGCATTCA CAGCTGACTT TGTGAGGCAG CTCTTGATAG GGCTACCCAA CCAAGCCCTC 
GGACTCACAC TGTGCAGTTT TATCAGTGCT CTGGGTGTAG ACATCATTGC TCAAGTAGAG 
GCAAAGGACT TTGGTGCCGA AAGCAAAGTT TCTGTTGATG ATCTCTGTAA GAAAGCGGTG 
GAACATAACA TCCAGATAGG GAAGTTCTCT CAGCTGGTTA TGAACAGGGC AACTGTGTTA 
GCAAGTTCTT ACGACACTGC CTGGAAGAAG CATGACTTGG TGCGAAGGCT AGAAACCAGT 
ATTTCTTCTT GTAAGACAAG CCTGCAGCGG GTTCAGCTGC ATATTGCCAT GTTTCAGTGG 
CAACATGAAG ATCTACTTAT CAATAGACCA CAAGCCATGT CAGTCACACC TCCCCCACGG 
TCTGCTATCC TAACCAGCAT GAAAAAGAAG CTGCATACCC TGAGCCAGAT TGAAACTTCT 
ATTGCAACAG TTCAGGAGAA GCTAGCTGCA CTTGAATCAA GTATTGAACA GCGACTCAAG 
TGGGCAGGTG GTGCCAACCC TGCATTGGCC CCTGTACTAC AAGATTTTGA AGCAACGATA 
GCTGAAAGAA GAAATCTTGT CCTTAAAGAG AGCCAAAGAG CAAGTCAGGT CACATTTCTC 
TGCAGCAATA TCATTCATTT TGAAAGTTTA CGAACAAGAA CTGCAGAAGC CTTAAACCTG 
GATGCGGCGT TATTTGAACT AATCAAGCGA TGTCAGCAGA TGTGTTCGTT TGCATCACAG 
TTTAACAGTT CAGTGTCTGA GTTAGAGCTT CGTTTATTAC AGAGAGTGGA CACTGGTCTT 
GAACATCCTA TTGGCAGCTC TGAATGGCTT TTGTCAGCAC ACAAACAGTT GACCCAGGAT 
ATGTCTACTC AGAGGGCAAT TCAGACAGAG AAAGAGCAGC AGATAGAAAC GGTCTGTGAA 
ACAATTCAGA ATCTGGTTGA TAATATAAAG ACTGTGCTCA CTGGTCATAA CCGACAGCTT 
GGAGATGTCA AACATCTCTT GAAAGCTATG GCTAAGGATG AAGAAGCTGC TCTGGCAGAT 
GGTGAAGATG TTCCCTATGA GAACAGTGTT AGGCAGTTTT TGGGTGAATA TAAATCATGG 
CAAGACAACA TTCAAACAGT TCTATTTACA TTAGTCCAGG CTATGGGTCA GGTTCGAAGT 
CAAGAACACG TTGAAATGCT CCAGGAAATC ACTCCCACCT TGAAAGAACT GAAAACACAA 
AGTCAGAGTA TCTATAATAA TTTAGTGAGT TTTGCATCAC CCTTAGTCAC CGATGCAACA 
AATGAATGTT CGAGTCCAAC GTCATCTGCT ACTTATCAGC CATCCTTCGC TGCAGCAGTC 
CGGAGTAACA CTGGCCAGAA GACTCAGCCT GATGTCATGT CACAGAATGC TAGAAAGCTG 
ATCCAAAAAA ATCTTGCTAC ATCAGCTGAT ACTCCACCAA GCACCGTTCC AGGAACTGGC 
AAGAGTGTTG CTTGTAGTCC TAAAAAGGCA GTCAGAGACC CTAAAACTGG GAAAGCGGTG 
CAAGAGAGAA ACTCCTATGC AGTGAGTGTG TGGAAGAGAG TGAAAGCCAA GTTAGAGGGC 
CGAGATGTTG ATCCGAATAG GAGGATGTCA GTTGCTGAAC AGGTTGACTA TGTCATTAAG 
GAAGCAACTA ATCTAGATAA CTTGGCTCAG CTGTATGAAG GTTGGACAGC CTGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SMG1
Entrez Gene ID	23049
Full Name	SMG1, nonsense mediated mRNA decay associated PI3K related kinase
Synonyms	61E3.4,ATX,LIP
General protein information	Preferred Names SMG1, nonsense mediated mRNA decay associated PI3K related kinase Names serine/threonine-protein kinase SMG1 PI-3-kinase-related kinase SMG-1 SMG1 phosphatidylinositol 3-kinase-related kinase lambda-interacting protein lambda/iota protein kinase C-interacting protein smg-1 homolog, phosphatidylinositol 3-kinase-related kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p12.3
Summary	This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternatively spliced transcript variants have been described, but their full-length nature has yet to be determined. [provided by RefSeq, Mar 2013].
Disorder MIM:	607032

Canis lupus familiaris (dog)	SMG1	XP_851552.2
Bos taurus (cattle)	SMG1	NP_001192844.1
Pan troglodytes (chimpanzee)	SMG1	XP_001152224.2
Homo sapiens (human)	SMG1	NP_055907.3
Rattus norvegicus (Norway rat)	Smg1	XP_002728837.2
Gallus gallus (chicken)	SMG1	XP_414907.3
Mus musculus (house mouse)	Smg1	NP_001026984.1
	LOC693542	XP_002808335.1
Danio rerio (zebrafish)	smg1	NP_001073513.2
Xenopus tropicalis (tropical clawed frog)	smg1	XP_002932267.2

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SMG1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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