RAB21 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAB21
Entrez Gene ID	23011
Full Name	RAB21, member RAS oncogene family
General protein information	Preferred Names RAB21, member RAS oncogene family Names ras-related protein Rab-21 GTP-binding protein RAB21
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q21.1
Summary	This gene belongs to the Rab family of monomeric GTPases, which are involved in the control of cellular membrane traffic. The encoded protein plays a role in the targeted trafficking of integrins via its association with integrin alpha tails. As a consequence, the encoded protein is involved in the regulation of cell adhesion and migration. Expression of this gene is associated with a poor prognosis for glioma patients. This gene is downregulated by the tumor suppressor miR-200b, and miRNA-200b is itself downregulated in glioma tissues. [provided by RefSeq, Nov 2015].
Disorder MIM:	612398

mRNA and Protein(s)

mRNA	Protein	Name
NM_014999.3	NP_055814.1	ras-related protein Rab-21
NM_014999.4	NP_055814.1	ras-related protein Rab-21

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAB21	XP_522469.2
	LOC718715	XP_001117520.1
Rattus norvegicus (Norway rat)	Rab21	NP_001004238.1
Danio rerio (zebrafish)	zgc:154045	NP_001070133.1
Mus musculus (house mouse)	Rab21	NP_077774.1
Gallus gallus (chicken)	RAB21	XP_001233648.1
Drosophila melanogaster (fruit fly)	Rab21	NP_001259785.1
Caenorhabditis elegans (roundworm)	rab-21	NP_495854.1
Homo sapiens (human)	RAB21	NP_055814.1
Canis lupus familiaris (dog)	RAB21	XP_003431485.2
Oryza sativa (rice)	Os05g0341600	NP_001055247.1
Xenopus tropicalis (tropical clawed frog)	rab21	NP_001016319.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Rab21, a Novel PS1 Interactor, Regulates ?-Secretase Activity via PS1 Subcellular Distribution.
Sun Z, Xie Y, Chen Y, Yang Q, Quan Z, Dai R, Qing H
Molecular neurobiology(2017 May)

Knockdown of Rab21 inhibits proliferation and induces apoptosis in human glioma cells.
Ge J, Chen Q, Liu B, Wang L, Zhang S, Ji B
Cellular & molecular biology letters2230(2017)

Starvation-induced MTMR13 and RAB21 activity regulates VAMP8 to promote autophagosome-lysosome fusion.
Jean S, Cox S, Nassari S, Kiger AA
EMBO reports16(3)297-311(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Starvation-induced MTMR13 and RAB21 activity regulates VAMP8 to promote autophagosome-lysosome fusion.
Title: Starvation-induced MTMR13 and RAB21 activity regulates VAMP8 to promote autophagosome-lysosome fusion.

High RAB21 expression is associated with glioma.
Title: miR-200b as a prognostic factor targets multiple members of RAB family in glioma.

Taken together, these data suggest that Rab21 plays a negative role in the EGF-mediated MAPK signaling pathway. Overexpression of Rab21 attenuated EGF-mediated mitogen-activated protein kinase (MAPK) signaling by inducing EGFR degradation.
Title: Rab21 attenuates EGF-mediated MAPK signaling through enhancing EGFR internalization and degradation.

show that TI-VAMP interacts with the Vps9 domain and ankyrin-repeat-containing protein Varp, a guanine nucleotide exchange factor of the small GTPase Rab21, through a specific domain herein called the interacting domain.
Title: Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth.

Results show that targeted trafficking of integrins to and from the cleavage furrow is required for successful cytokinesis, and that this is regulated by Rab21.
Title: Integrin trafficking regulated by Rab21 is necessary for cytokinesis.

The following RAB21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAB21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30805	NM_014999.4 Latest version!	Homo sapiens RAB21, member RAS oncogene family (RAB21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu30805	NM_014999.3	Homo sapiens RAB21, member RAS oncogene family (RAB21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAB21 ( NM_014999.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055814.1 Homo sapiens RAB21, member RAS oncogene family (RAB21), mRNA.

Price & Availability↑

CloneID	OHu30805
Clone ID Related Accession (Same CDS sequence)	NM_014999.3 , NM_014999.4
Accession Version	NM_014999.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-25
Organism	Homo sapiens(human)
Product	ras-related protein Rab-21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB990575.1, AF091035.1 and AC089984.23. On Nov 14, 2015 this sequence version replaced NM_014999.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.92650.1, SRR1803611.205270.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGG CCGGCGGCGG CGGCGGCGGG GCGGCGGCGG CGGGCCGAGC CTACTCGTTC 
AAGGTGGTGC TGCTGGGGGA AGGCTGCGTG GGGAAGACGT CGCTGGTGCT GCGCTACTGC 
GAGAACAAGT TTAACGACAA GCACATCACC ACTCTGCAGG CATCATTCTT AACAAAGAAG 
TTAAATATTG GTGGGAAAAG AGTAAACCTT GCCATATGGG ATACGGCAGG TCAAGAGAGA 
TTCCATGCAT TGGGTCCAAT TTACTACAGA GATTCAAATG GAGCGATTTT AGTTTATGAC 
ATAACAGATG AAGATTCTTT TCAGAAGGTA AAAAACTGGG TCAAAGAATT ACGGAAAATG 
TTGGGAAATG AAATCTGTTT ATGTATAGTT GGTAATAAAA TAGACTTGGA AAAGGAGAGA 
CATGTTTCCA TTCAAGAAGC AGAGTCGTAT GCAGAATCTG TGGGAGCAAA ACATTATCAT 
ACTTCAGCCA AACAGAACAA AGGAATTGAG GAACTCTTTC TTGACCTTTG TAAAAGGATG 
ATAGAAACAG CACAAGTGGA TGAGAGAGCA AAAGGCAATG GCTCTAGTCA GCCGGGAACT 
GCAAGGCGAG GTGTACAGAT TATTGATGAT GAACCTCAAG CCCAGACCAG TGGTGGAGGG 
TGCTGTTCTT CTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055814.1
CDS	268..945
Translation	MAAAGGGGGGAAAAGRAYSFKVVLLGEGCVGKTSLVLRYCENKFNDKHITTLQASFLTKKLNIGGKRVNLAIWDTAGQERFHALGPIYYRDSNGAILVYDITDEDSFQKVKNWVKELRKMLGNEICLCIVGNKIDLEKERHVSIQEAESYAESVGAKHYHTSAKQNKGIEELFLDLCKRMIETAQVDERAKGNGSSQPGTARRGVQIIDDEPQAQTSGGGCCSSG

Target ORF information:

RefSeq Version	NM_014999.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RAB21, member RAS oncogene family (RAB21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014999.3

ORF Insert Sequence:

ATGGCTGCGG CCGGCGGCGG CGGCGGCGGG GCGGCGGCGG CGGGCCGAGC CTACTCGTTC 
AAGGTGGTGC TGCTGGGGGA AGGCTGCGTG GGGAAGACGT CGCTGGTGCT GCGCTACTGC 
GAGAACAAGT TTAACGACAA GCACATCACC ACTCTGCAGG CATCATTCTT AACAAAGAAG 
TTAAATATTG GTGGGAAAAG AGTAAACCTT GCCATATGGG ATACGGCAGG TCAAGAGAGA 
TTCCATGCAT TGGGTCCAAT TTACTACAGA GATTCAAATG GAGCGATTTT AGTTTATGAC 
ATAACAGATG AAGATTCTTT TCAGAAGGTA AAAAACTGGG TCAAAGAATT ACGGAAAATG 
TTGGGAAATG AAATCTGTTT ATGTATAGTT GGTAATAAAA TAGACTTGGA AAAGGAGAGA 
CATGTTTCCA TTCAAGAAGC AGAGTCGTAT GCAGAATCTG TGGGAGCAAA ACATTATCAT 
ACTTCAGCCA AACAGAACAA AGGAATTGAG GAACTCTTTC TTGACCTTTG TAAAAGGATG 
ATAGAAACAG CACAAGTGGA TGAGAGAGCA AAAGGCAATG GCTCTAGTCA GCCGGGAACT 
GCAAGGCGAG GTGTACAGAT TATTGATGAT GAACCTCAAG CCCAGACCAG TGGTGGAGGG 
TGCTGTTCTT CTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAB21 ( NM_014999.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055814.1 Homo sapiens RAB21, member RAS oncogene family (RAB21), mRNA.

Price & Availability↑

CloneID	OHu30805
Clone ID Related Accession (Same CDS sequence)	NM_014999.3 , NM_014999.4
Accession Version	NM_014999.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	ras-related protein Rab-21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB990575.1, AF091035.1 and AC089984.23. On Nov 23, 2018 this sequence version replaced NM_014999.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.92650.1, SRR1803611.205270.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261263.5/ ENSP00000261263.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGCGG CCGGCGGCGG CGGCGGCGGG GCGGCGGCGG CGGGCCGAGC CTACTCGTTC 
AAGGTGGTGC TGCTGGGGGA AGGCTGCGTG GGGAAGACGT CGCTGGTGCT GCGCTACTGC 
GAGAACAAGT TTAACGACAA GCACATCACC ACTCTGCAGG CATCATTCTT AACAAAGAAG 
TTAAATATTG GTGGGAAAAG AGTAAACCTT GCCATATGGG ATACGGCAGG TCAAGAGAGA 
TTCCATGCAT TGGGTCCAAT TTACTACAGA GATTCAAATG GAGCGATTTT AGTTTATGAC 
ATAACAGATG AAGATTCTTT TCAGAAGGTA AAAAACTGGG TCAAAGAATT ACGGAAAATG 
TTGGGAAATG AAATCTGTTT ATGTATAGTT GGTAATAAAA TAGACTTGGA AAAGGAGAGA 
CATGTTTCCA TTCAAGAAGC AGAGTCGTAT GCAGAATCTG TGGGAGCAAA ACATTATCAT 
ACTTCAGCCA AACAGAACAA AGGAATTGAG GAACTCTTTC TTGACCTTTG TAAAAGGATG 
ATAGAAACAG CACAAGTGGA TGAGAGAGCA AAAGGCAATG GCTCTAGTCA GCCGGGAACT 
GCAAGGCGAG GTGTACAGAT TATTGATGAT GAACCTCAAG CCCAGACCAG TGGTGGAGGG 
TGCTGTTCTT CTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055814.1
CDS	268..945
Translation	MAAAGGGGGGAAAAGRAYSFKVVLLGEGCVGKTSLVLRYCENKFNDKHITTLQASFLTKKLNIGGKRVNLAIWDTAGQERFHALGPIYYRDSNGAILVYDITDEDSFQKVKNWVKELRKMLGNEICLCIVGNKIDLEKERHVSIQEAESYAESVGAKHYHTSAKQNKGIEELFLDLCKRMIETAQVDERAKGNGSSQPGTARRGVQIIDDEPQAQTSGGGCCSSG

Target ORF information:

RefSeq Version	NM_014999.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RAB21, member RAS oncogene family (RAB21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014999.4

ORF Insert Sequence:

ATGGCTGCGG CCGGCGGCGG CGGCGGCGGG GCGGCGGCGG CGGGCCGAGC CTACTCGTTC 
AAGGTGGTGC TGCTGGGGGA AGGCTGCGTG GGGAAGACGT CGCTGGTGCT GCGCTACTGC 
GAGAACAAGT TTAACGACAA GCACATCACC ACTCTGCAGG CATCATTCTT AACAAAGAAG 
TTAAATATTG GTGGGAAAAG AGTAAACCTT GCCATATGGG ATACGGCAGG TCAAGAGAGA 
TTCCATGCAT TGGGTCCAAT TTACTACAGA GATTCAAATG GAGCGATTTT AGTTTATGAC 
ATAACAGATG AAGATTCTTT TCAGAAGGTA AAAAACTGGG TCAAAGAATT ACGGAAAATG 
TTGGGAAATG AAATCTGTTT ATGTATAGTT GGTAATAAAA TAGACTTGGA AAAGGAGAGA 
CATGTTTCCA TTCAAGAAGC AGAGTCGTAT GCAGAATCTG TGGGAGCAAA ACATTATCAT 
ACTTCAGCCA AACAGAACAA AGGAATTGAG GAACTCTTTC TTGACCTTTG TAAAAGGATG 
ATAGAAACAG CACAAGTGGA TGAGAGAGCA AAAGGCAATG GCTCTAGTCA GCCGGGAACT 
GCAAGGCGAG GTGTACAGAT TATTGATGAT GAACCTCAAG CCCAGACCAG TGGTGGAGGG 
TGCTGTTCTT CTGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Rab21, a Novel PS1 Interactor, Regulates ?-Secretase Activity via PS1 Subcellular Distribution.
Molecular neurobiology(2017 May)
Sun Z,Xie Y,Chen Y,Yang Q,Quan Z,Dai R,Qing H

Knockdown of Rab21 inhibits proliferation and induces apoptosis in human glioma cells.
Cellular & molecular biology letters2230(2017)
Ge J,Chen Q,Liu B,Wang L,Zhang S,Ji B

Starvation-induced MTMR13 and RAB21 activity regulates VAMP8 to promote autophagosome-lysosome fusion.
EMBO reports16(3)297-311(2015 Mar)
Jean S,Cox S,Nassari S,Kiger AA