DIO3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIO3
Entrez Gene ID	1735
Full Name	iodothyronine deiodinase 3
Synonyms	5DIII,D3,DIOIII,TXDI3
General protein information	Preferred Names iodothyronine deiodinase 3 Names thyroxine 5-deiodinase deiodinase, iodothyronine type III iodothyronine deiodinase, placental type thyroxine deiodinase type III (selenoprotein) type 3 DI type 3 iodothyronine selenodeiodinase type-III 5' deiodinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q32.31
Summary	The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in pregnant uterus, placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It regulates circulating fetal thyroid hormone concentrations, and thus plays a critical role in mammalian development. Knockout mice lacking this gene exhibit abnormalities related to development and reproduction, and increased activity of this enzyme in infants with hemangiomas causes severe hypothyroidism. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, May 2016].
Disorder MIM:	601038

mRNA and Protein(s)

mRNA	Protein	Name
NM_001362.3	NP_001353.4	thyroxine 5-deiodinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIO3	NP_001353.4
Rattus norvegicus (Norway rat)	Dio3	NP_058906.3
Gallus gallus (chicken)	DIO3	NP_001116120.1
Xenopus tropicalis (tropical clawed frog)	dio3	NP_001107139.2
	DIO3	NP_001116121.1
Canis lupus familiaris (dog)	DIO3	NP_001157660.1
Danio rerio (zebrafish)	dio3a	NP_001242932.1
Bos taurus (cattle)	DIO3	NP_001010993.2
Mus musculus (house mouse)	Dio3	NP_742117.2
Danio rerio (zebrafish)	dio3b	NP_001171406.2

Gene Ontology
Bibliography

Related articles in PubMed

The Thyroid Hormone Inactivating Type 3 Deiodinase Is Essential for Optimal Neutrophil Function: Observations From Three Species.
van der Spek AH, Jim KK, Karaczyn A, van Beeren HC, Ackermans MT, Darras VM, Vandenbroucke-Grauls CMJE, Hernandez A, Brouwer MC, Fliers E, van de Beek D, Boelen A
Endocrinology159(2)826-835(2018 Feb)

Assessment of type 1 and type 3 deiodinase expression levels in depressive disorders.
Ga?ecka E, Kumor-Kisielewska A, Orzechowska A, Maes M, G?rski P, Szemraj J
Acta neurobiologiae experimentalis77(3)225-235(2017)

Deregulation of small non-coding RNAs at the DLK1-DIO3 imprinted locus predicts lung cancer patient outcome.
Enfield KS, Martinez VD, Marshall EA, Stewart GL, Kung SH, Enterina JR, Lam WL
Oncotarget7(49)80957-80966(2016 Dec)

Deiodination in cancer growth: the role of type III deiodinase.
Sibilio A, Ambrosio R, Bonelli C, De Stefano MA, Torre V, Dentice M, Salvatore D
Minerva endocrinologica37(4)315-27(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In neonatal skin, DIO3 exhibited a high degree of monoallelic expression from the paternal allele.
Title: Genomic imprinting of DIO3, a candidate gene for the syndrome associated with human uniparental disomy of chromosome 14.

Presence and subcellular location of D3 in human neutrophils for the first time and propose a model, in which D3 plays a role in the bacterial killing capacity of neutrophils either through generation of iodide for the myeloperoxidase system or through modulation of intracellular Thyroid hormone bioavailability.
Title: The Thyroid Hormone Inactivating Enzyme Type 3 Deiodinase is Present in Bactericidal Granules and the Cytoplasm of Human Neutrophils.

D3 is a critical factor in testis development and in testicular protection from thyrotoxicosis.
Title: The Type 3 Deiodinase Is a Critical Determinant of Appropriate Thyroid Hormone Action in the Developing Testis.

MAPK and SHH pathways modulate type 3 deiodinase expression in papillary thyroid carcinoma
Title: MAPK and SHH pathways modulate type 3 deiodinase expression in papillary thyroid carcinoma.

Thyroid hormone deiodinases D1, D2, and D3 are differentially expressed in endothelial cells following thyroid hormone exposure.
Title: Thyroid hormone deiodinases D1, D2, and D3 are expressed in human endothelial dermal microvascular line: effects of thyroid hormones.

The following DIO3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIO3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu24700	NM_001362.3 Latest version!	Homo sapiens iodothyronine deiodinase 3 (DIO3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIO3 ( NM_001362.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353.4 Homo sapiens iodothyronine deiodinase 3 (DIO3), mRNA.

Price & Availability↑

CloneID	OHu24700
Clone ID Related Accession (Same CDS sequence)	NM_001362.3
Accession Version	NM_001362.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	thyroxine 5-deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX161470.1 and BC017717.1. This sequence is a reference standard in the RefSeqGene project. On May 23, 2007 this sequence version replaced NM_001362.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BX161470.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 7593630 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCTCGCC AGGCCACGTC GCGGTTGGTG GTCGGAGAGG GCGAGGGGTC CCAGGGGGCT 
TCGGGGCCTG CAGCCACCAT GCTCCGCTCC CTGCTGCTTC ACTCCTTGAG GCTCTGCGCC 
CAGACCGCCT CGTGCCTCGT GCTCTTCCCG CGCTTCCTCG GCACGGCCTT CATGCTCTGG 
CTTCTCGATT TCTTGTGTAT CCGCAAGCAT TTCCTGGGCC GCCGCCGCCG GGGGCAGCCC 
GAGCCCGAAG TGGAGCTCAA CAGTGAAGGC GAGGAGGTGC CTCCCGATGA CCCGCCCATC 
TGCGTGTCCG ACGACAACCG CCTGTGCACC CTGGCGTCGC TCAAGGCGGT GTGGCATGGC 
CAGAAGTTGG ATTTCTTCAA GCAGGCGCAC GAGGGCGGTC CGGCGCCCAA CTCCGAGGTG 
GTTCTGCCCG ACGGCTTCCA GAGCCAGCAC ATCCTCGACT ACGCGCAAGG GAACCGCCCG 
CTGGTTCTCA ATTTCGGCAG CTGCACCTGA CCACCGTTCA TGGCGCGCAT GAGCGCCTTC 
CAGCGCCTGG TCACTAAGTA CCAGCGCGAC GTCGACTTCC TCATCATCTA CATCGAGGAA 
GCGCACCCCT CCGACGGCTG GGTCACCACG GACTCTCCCT ACATCATCCC ACAGCACCGG 
AGCCTGGAGG ACCGGGTCAG CGCAGCGAGG GTACTGCAGC AAGGTGCACC CGGCTGCGCT 
CTGGTCCTCG ACACCATGGC CAACTCCAGC AGCTCGGCCT ATGGCGCCTA CTTCGAGCGT 
CTCTATGTCA TCCAGAGTGG CACTATTATG TACCAGGGCG GCCGTGGCCC CGACGGCTAC 
CAGGTCTCTG AGCTGCGCAC TTGGTTGGAA CGCTATGATG AGCAACTGCA CGGCGCTCGG 
CCCCGGAGGG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353.4
CDS	147..1061
Translation	MPRQATSRLVVGEGEGSQGASGPAATMLRSLLLHSLRLCAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPEPEVELNSEGEEVPPDDPPICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGGPAPNSEVVLPDGFQSQHILDYAQGNRPLVLNFGSCT*PPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYIIPQHRSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYDEQLHGARPRRV

Target ORF information:

RefSeq Version	NM_001362.3
Organism	Homo sapiens(human)
Definition	Homo sapiens iodothyronine deiodinase 3 (DIO3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362.3

ORF Insert Sequence:

ATGCCTCGCC AGGCCACGTC GCGGTTGGTG GTCGGAGAGG GCGAGGGGTC CCAGGGGGCT 
TCGGGGCCTG CAGCCACCAT GCTCCGCTCC CTGCTGCTTC ACTCCTTGAG GCTCTGCGCC 
CAGACCGCCT CGTGCCTCGT GCTCTTCCCG CGCTTCCTCG GCACGGCCTT CATGCTCTGG 
CTTCTCGATT TCTTGTGTAT CCGCAAGCAT TTCCTGGGCC GCCGCCGCCG GGGGCAGCCC 
GAGCCCGAAG TGGAGCTCAA CAGTGAAGGC GAGGAGGTGC CTCCCGATGA CCCGCCCATC 
TGCGTGTCCG ACGACAACCG CCTGTGCACC CTGGCGTCGC TCAAGGCGGT GTGGCATGGC 
CAGAAGTTGG ATTTCTTCAA GCAGGCGCAC GAGGGCGGTC CGGCGCCCAA CTCCGAGGTG 
GTTCTGCCCG ACGGCTTCCA GAGCCAGCAC ATCCTCGACT ACGCGCAAGG GAACCGCCCG 
CTGGTTCTCA ATTTCGGCAG CTGCACCTGA CCACCGTTCA TGGCGCGCAT GAGCGCCTTC 
CAGCGCCTGG TCACTAAGTA CCAGCGCGAC GTCGACTTCC TCATCATCTA CATCGAGGAA 
GCGCACCCCT CCGACGGCTG GGTCACCACG GACTCTCCCT ACATCATCCC ACAGCACCGG 
AGCCTGGAGG ACCGGGTCAG CGCAGCGAGG GTACTGCAGC AAGGTGCACC CGGCTGCGCT 
CTGGTCCTCG ACACCATGGC CAACTCCAGC AGCTCGGCCT ATGGCGCCTA CTTCGAGCGT 
CTCTATGTCA TCCAGAGTGG CACTATTATG TACCAGGGCG GCCGTGGCCC CGACGGCTAC 
CAGGTCTCTG AGCTGCGCAC TTGGTTGGAA CGCTATGATG AGCAACTGCA CGGCGCTCGG 
CCCCGGAGGG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Thyroid Hormone Inactivating Type 3 Deiodinase Is Essential for Optimal Neutrophil Function: Observations From Three Species.
Endocrinology159(2)826-835(2018 Feb)
van der Spek AH,Jim KK,Karaczyn A,van Beeren HC,Ackermans MT,Darras VM,Vandenbroucke-Grauls CMJE,Hernandez A,Brouwer MC,Fliers E,van de Beek D,Boelen A

Assessment of type 1 and type 3 deiodinase expression levels in depressive disorders.
Acta neurobiologiae experimentalis77(3)225-235(2017)
Ga?ecka E,Kumor-Kisielewska A,Orzechowska A,Maes M,G?rski P,Szemraj J

Deregulation of small non-coding RNAs at the DLK1-DIO3 imprinted locus predicts lung cancer patient outcome.
Oncotarget7(49)80957-80966(2016 Dec)
Enfield KS,Martinez VD,Marshall EA,Stewart GL,Kung SH,Enterina JR,Lam WL

Deiodination in cancer growth: the role of type III deiodinase.
Minerva endocrinologica37(4)315-27(2012 Dec)
Sibilio A,Ambrosio R,Bonelli C,De Stefano MA,Torre V,Dentice M,Salvatore D

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DIO3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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