GSDME cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GSDME
Entrez Gene ID	1687
Full Name	gasdermin E
Synonyms	DFNA5,ICERE-1
General protein information	Preferred Names gasdermin E Names gasdermin-E non-syndromic hearing impairment protein 5 DFNA5, deafness associated tumor suppressor inversely correlated with estrogen receptor expression 1 nonsyndromic hearing impairment protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p15.3
Summary	Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	608798
Disorder Html:	Deafness, autosomal dominant 5, 600994 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004403.3	NP_004394.1	gasdermin-E isoform a
XM_024446670.1	XP_024302438.1	gasdermin-E isoform X1
XM_017011802.1	XP_016867291.1	gasdermin-E isoform X2
NM_004403.2	NP_004394.1	gasdermin-E isoform a
NM_001127454.1	NP_001120926.1	gasdermin-E isoform b
NM_001127454.2	NP_001120926.1	gasdermin-E isoform b
NM_001127453.1	NP_001120925.1	gasdermin-E isoform a
NM_001127453.2	NP_001120925.1	gasdermin-E isoform a

Pathways from BioSystems

Homologies

Homo sapiens (human)	DFNA5	NP_004394.1
Mus musculus (house mouse)	Dfna5	NP_061239.1
Gallus gallus (chicken)	DFNA5	NP_001006361.1
Rattus norvegicus (Norway rat)	Dfna5	NP_001178678.1
Pan troglodytes (chimpanzee)	DFNA5	XP_001159685.2
	DFNA5	XP_001096330.1
Bos taurus (cattle)	DFNA5	NP_001180041.1
Danio rerio (zebrafish)	dfna5	NP_001001947.1
Canis lupus familiaris (dog)	DFNA5	XP_853956.2
Xenopus tropicalis (tropical clawed frog)	dfna5	XP_002933445.2

Gene Ontology

The following GSDME gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GSDME cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25942	NM_001127453.2 Latest version!	Homo sapiens gasdermin E (GSDME), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu25942	NM_004403.3 Latest version!	Homo sapiens gasdermin E (GSDME), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu26331	NM_001127454.2 Latest version!	Homo sapiens gasdermin E (GSDME), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu25942	XM_024446670.1 Latest version!	Homo sapiens gasdermin E (GSDME), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu26331	XM_017011802.1 Latest version!	Homo sapiens gasdermin E (GSDME), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu25942	NM_004403.2	Homo sapiens gasdermin E (GSDME), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu26331	NM_001127454.1	Homo sapiens gasdermin E (GSDME), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu25942	NM_001127453.1	Homo sapiens gasdermin E (GSDME), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GSDME ( NM_004403.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004394.1 Homo sapiens gasdermin E (GSDME), transcript variant 1, mRNA.

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CloneID	OHu25942
Clone ID Related Accession (Same CDS sequence)	NM_004403.3 , XM_024446670.1 , NM_004403.2 , NM_001127453.1 , NM_001127453.2
Accession Version	NM_004403.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	gasdermin-E isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC003093.1, AF073308.1, BC125065.1 and AF131765.1. On Nov 23, 2018 this sequence version replaced NM_004403.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.74450.1, SRR1803615.207378.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG 
AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT 
GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC 
GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG 
CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG 
CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA 
AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG 
AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT 
GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT 
GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT 
GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG 
CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT 
CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA 
CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG 
GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT 
GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC 
ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG 
CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG 
CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA 
GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT 
CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA 
GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG 
AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT 
CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004394.1
CDS	253..1743
Translation	MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSPVVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCALGREHS

Target ORF information:

RefSeq Version	NM_004403.3
Organism	Homo sapiens(human)
Definition	Homo sapiens gasdermin E (GSDME), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004403.3

ORF Insert Sequence:

ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG 
AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT 
GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC 
GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG 
CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG 
CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA 
AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG 
AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT 
GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT 
GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT 
GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG 
CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT 
CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA 
CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG 
GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT 
GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC 
ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG 
CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG 
CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA 
GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT 
CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA 
GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG 
AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT 
CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GSDME ( NM_004403.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004394.1 Homo sapiens gasdermin E (GSDME), transcript variant 1, mRNA.

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CloneID	OHu25942
Clone ID Related Accession (Same CDS sequence)	NM_004403.3 , XM_024446670.1 , NM_004403.2 , NM_001127453.1 , NM_001127453.2
Accession Version	NM_004403.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	gasdermin-E isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC003093.1, AF073308.1, BC125065.1 and AF131765.1. This sequence is a reference standard in the RefSeqGene project. On Oct 26, 2006 this sequence version replaced NM_004403.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.74450.1, SRR1803614.243516.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG 
AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT 
GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC 
GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG 
CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG 
CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA 
AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG 
AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT 
GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT 
GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT 
GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG 
CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT 
CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA 
CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG 
GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT 
GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC 
ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG 
CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG 
CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA 
GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT 
CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA 
GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG 
AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT 
CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004394.1
CDS	334..1824
Translation	MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSPVVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCALGREHS

Target ORF information:

RefSeq Version	NM_004403.2
Organism	Homo sapiens(human)
Definition	Homo sapiens gasdermin E (GSDME), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004403.2

ORF Insert Sequence:

ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG 
AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT 
GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC 
GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG 
CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG 
CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA 
AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG 
AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT 
GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT 
GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT 
GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG 
CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT 
CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA 
CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG 
GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT 
GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC 
ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG 
CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG 
CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA 
GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT 
CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA 
GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG 
AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT 
CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GSDME ( NM_001127453.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120925.1 Homo sapiens gasdermin E (GSDME), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25942
Clone ID Related Accession (Same CDS sequence)	NM_004403.3 , XM_024446670.1 , NM_004403.2 , NM_001127453.1 , NM_001127453.2
Accession Version	NM_001127453.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-08
Organism	Homo sapiens(human)
Product	gasdermin-E isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA820184.1, BC125065.1 and AF131765.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.287696.1, SRR1163657.205142.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG 
AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT 
GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC 
GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG 
CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG 
CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA 
AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG 
AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT 
GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT 
GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT 
GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG 
CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT 
CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA 
CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG 
GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT 
GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC 
ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG 
CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG 
CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA 
GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT 
CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA 
GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG 
AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT 
CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120925.1
CDS	89..1579
Translation	MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSPVVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCALGREHS

Target ORF information:

RefSeq Version	NM_001127453.1
Organism	Homo sapiens(human)
Definition	Homo sapiens gasdermin E (GSDME), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127453.1

ORF Insert Sequence:

ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG 
AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT 
GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC 
GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG 
CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG 
CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA 
AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG 
AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT 
GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT 
GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT 
GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG 
CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT 
CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA 
CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG 
GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT 
GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC 
ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG 
CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG 
CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA 
GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT 
CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA 
GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG 
AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT 
CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GSDME ( NM_001127453.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120925.1 Homo sapiens gasdermin E (GSDME), transcript variant 2, mRNA.

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CloneID	OHu25942
Clone ID Related Accession (Same CDS sequence)	NM_004403.3 , XM_024446670.1 , NM_004403.2 , NM_001127453.1 , NM_001127453.2
Accession Version	NM_001127453.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	gasdermin-E isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA820184.1, BC125065.1 and AF131765.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001127453.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.124413.1, SRR3476690.493329.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000645220.1/ ENSP00000494186.1 RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG 
AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT 
GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC 
GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG 
CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG 
CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA 
AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG 
AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT 
GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT 
GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT 
GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG 
CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT 
CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA 
CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG 
GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT 
GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC 
ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG 
CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG 
CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA 
GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT 
CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA 
GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG 
AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT 
CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120925.1
CDS	89..1579
Translation	MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSPVVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCALGREHS

Target ORF information:

RefSeq Version	NM_001127453.2
Organism	Homo sapiens(human)
Definition	Homo sapiens gasdermin E (GSDME), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127453.2

ORF Insert Sequence:

ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG 
AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT 
GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC 
GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG 
CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG 
CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA 
AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG 
AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT 
GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT 
GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT 
GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG 
CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT 
CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA 
CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG 
GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT 
GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC 
ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG 
CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG 
CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA 
GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT 
CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA 
GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG 
AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT 
CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GSDME ( NM_001127454.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120926.1 Homo sapiens gasdermin E (GSDME), transcript variant 3, mRNA.

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CloneID	OHu26331
Clone ID Related Accession (Same CDS sequence)	XM_017011802.1 , NM_001127454.1 , NM_001127454.2
Accession Version	NM_001127454.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-08
Organism	Homo sapiens(human)
Product	gasdermin-E isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA908548.1, DA197867.1, BC125066.1, BC125065.1 and AF131765.1. Transcript Variant: This variant (3) lacks an alternate exon compared to variant 1, resulting in an isoform (b) that uses a downstream start site compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.170886.1, SRR1803613.169648.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAGT GTGTGATCTC TGAGCACATG CAGGTCGAGG AGAAGTGTGG TGGCATCGTG 
GGCATCCAGA CCAAGACGGT GCAGGTGTCA GCGACGGAGG ATGGGAATGT CACCAAGGAC 
TCCAACGTGG TGCTGGAGAT CCCAGCTGCC ACCACCATTG CCTACGGTGT CATTGAGTTA 
TACGTGAAAC TGGACGGCCA GTTCGAGTTC TGCCTTCTCC GAGGGAAGCA AGGTGGCTTC 
GAGAACAAGA AGAGAATTGA CTCTGTCTAC CTGGACCCCC TGGTCTTTCG AGAGTTTGCA 
TTCATAGACA TGCCAGATGC TGCGCATGGG ATATCTTCCC AGGATGGACC ATTAAGTGTT 
TTAAAGCAAG CGACCCTGCT CCTGGAGAGG AATTTCCATC CATTTGCGGA GCTGCCTGAG 
CCACAACAGA CAGCTTTGAG TGACATCTTC CAGGCGGTCC TATTTGATGA TGAACTACTC 
ATGGTCCTGG AACCAGTGTG CGATGACCTG GTCAGCGGCC TCTCGCCCAC AGTGGCGGTG 
CTGGGGGAGC TGAAGCCCCG GCAGCAGCAG GACCTTGTGG CCTTCCTGCA GCTGGTGGGG 
TGCAGCTTAC AGGGTGGGTG TCCGGGCCCC GAGGATGCAG GCAGCAAGCA GCTGTTTATG 
ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG ATAGCGCAGC AGCTCTGCTG 
GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC ACTTGCTTCG TGCTCTGTCT 
GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC CCCTGAAAGA TACAGAAAGG 
TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA GTCTGGAGAG ACTGAAGTCA 
TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC CACTGCTTCT TTGTATAACC 
CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120926.1
CDS	596..1594
Translation	MQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCALGREHS

Target ORF information:

RefSeq Version	NM_001127454.1
Organism	Homo sapiens(human)
Definition	Homo sapiens gasdermin E (GSDME), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127454.1

ORF Insert Sequence:

ATGCAGAAGT GTGTGATCTC TGAGCACATG CAGGTCGAGG AGAAGTGTGG TGGCATCGTG 
GGCATCCAGA CCAAGACGGT GCAGGTGTCA GCGACGGAGG ATGGGAATGT CACCAAGGAC 
TCCAACGTGG TGCTGGAGAT CCCAGCTGCC ACCACCATTG CCTACGGTGT CATTGAGTTA 
TACGTGAAAC TGGACGGCCA GTTCGAGTTC TGCCTTCTCC GAGGGAAGCA AGGTGGCTTC 
GAGAACAAGA AGAGAATTGA CTCTGTCTAC CTGGACCCCC TGGTCTTTCG AGAGTTTGCA 
TTCATAGACA TGCCAGATGC TGCGCATGGG ATATCTTCCC AGGATGGACC ATTAAGTGTT 
TTAAAGCAAG CGACCCTGCT CCTGGAGAGG AATTTCCATC CATTTGCGGA GCTGCCTGAG 
CCACAACAGA CAGCTTTGAG TGACATCTTC CAGGCGGTCC TATTTGATGA TGAACTACTC 
ATGGTCCTGG AACCAGTGTG CGATGACCTG GTCAGCGGCC TCTCGCCCAC AGTGGCGGTG 
CTGGGGGAGC TGAAGCCCCG GCAGCAGCAG GACCTTGTGG CCTTCCTGCA GCTGGTGGGG 
TGCAGCTTAC AGGGTGGGTG TCCGGGCCCC GAGGATGCAG GCAGCAAGCA GCTGTTTATG 
ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG ATAGCGCAGC AGCTCTGCTG 
GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC ACTTGCTTCG TGCTCTGTCT 
GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC CCCTGAAAGA TACAGAAAGG 
TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA GTCTGGAGAG ACTGAAGTCA 
TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC CACTGCTTCT TTGTATAACC 
CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GSDME ( NM_001127454.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120926.1 Homo sapiens gasdermin E (GSDME), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu26331
Clone ID Related Accession (Same CDS sequence)	XM_017011802.1 , NM_001127454.1 , NM_001127454.2
Accession Version	NM_001127454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	gasdermin-E isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA908548.1, DA197867.1, BC125066.1, BC125065.1 and AF131765.1. On Jun 1, 2019 this sequence version replaced NM_001127454.1. Transcript Variant: This variant (3) lacks an alternate exon compared to variant 1, resulting in an isoform (b) that uses a downstream start site compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.170886.1, SRR1803613.281346.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAAGT GTGTGATCTC TGAGCACATG CAGGTCGAGG AGAAGTGTGG TGGCATCGTG 
GGCATCCAGA CCAAGACGGT GCAGGTGTCA GCGACGGAGG ATGGGAATGT CACCAAGGAC 
TCCAACGTGG TGCTGGAGAT CCCAGCTGCC ACCACCATTG CCTACGGTGT CATTGAGTTA 
TACGTGAAAC TGGACGGCCA GTTCGAGTTC TGCCTTCTCC GAGGGAAGCA AGGTGGCTTC 
GAGAACAAGA AGAGAATTGA CTCTGTCTAC CTGGACCCCC TGGTCTTTCG AGAGTTTGCA 
TTCATAGACA TGCCAGATGC TGCGCATGGG ATATCTTCCC AGGATGGACC ATTAAGTGTT 
TTAAAGCAAG CGACCCTGCT CCTGGAGAGG AATTTCCATC CATTTGCGGA GCTGCCTGAG 
CCACAACAGA CAGCTTTGAG TGACATCTTC CAGGCGGTCC TATTTGATGA TGAACTACTC 
ATGGTCCTGG AACCAGTGTG CGATGACCTG GTCAGCGGCC TCTCGCCCAC AGTGGCGGTG 
CTGGGGGAGC TGAAGCCCCG GCAGCAGCAG GACCTTGTGG CCTTCCTGCA GCTGGTGGGG 
TGCAGCTTAC AGGGTGGGTG TCCGGGCCCC GAGGATGCAG GCAGCAAGCA GCTGTTTATG 
ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG ATAGCGCAGC AGCTCTGCTG 
GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC ACTTGCTTCG TGCTCTGTCT 
GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC CCCTGAAAGA TACAGAAAGG 
TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA GTCTGGAGAG ACTGAAGTCA 
TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC CACTGCTTCT TTGTATAACC 
CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120926.1
CDS	515..1513
Translation	MQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCALGREHS

Target ORF information:

RefSeq Version	NM_001127454.2
Organism	Homo sapiens(human)
Definition	Homo sapiens gasdermin E (GSDME), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127454.2

ORF Insert Sequence:

ATGCAGAAGT GTGTGATCTC TGAGCACATG CAGGTCGAGG AGAAGTGTGG TGGCATCGTG 
GGCATCCAGA CCAAGACGGT GCAGGTGTCA GCGACGGAGG ATGGGAATGT CACCAAGGAC 
TCCAACGTGG TGCTGGAGAT CCCAGCTGCC ACCACCATTG CCTACGGTGT CATTGAGTTA 
TACGTGAAAC TGGACGGCCA GTTCGAGTTC TGCCTTCTCC GAGGGAAGCA AGGTGGCTTC 
GAGAACAAGA AGAGAATTGA CTCTGTCTAC CTGGACCCCC TGGTCTTTCG AGAGTTTGCA 
TTCATAGACA TGCCAGATGC TGCGCATGGG ATATCTTCCC AGGATGGACC ATTAAGTGTT 
TTAAAGCAAG CGACCCTGCT CCTGGAGAGG AATTTCCATC CATTTGCGGA GCTGCCTGAG 
CCACAACAGA CAGCTTTGAG TGACATCTTC CAGGCGGTCC TATTTGATGA TGAACTACTC 
ATGGTCCTGG AACCAGTGTG CGATGACCTG GTCAGCGGCC TCTCGCCCAC AGTGGCGGTG 
CTGGGGGAGC TGAAGCCCCG GCAGCAGCAG GACCTTGTGG CCTTCCTGCA GCTGGTGGGG 
TGCAGCTTAC AGGGTGGGTG TCCGGGCCCC GAGGATGCAG GCAGCAAGCA GCTGTTTATG 
ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG ATAGCGCAGC AGCTCTGCTG 
GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC ACTTGCTTCG TGCTCTGTCT 
GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC CCCTGAAAGA TACAGAAAGG 
TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA GTCTGGAGAG ACTGAAGTCA 
TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC CACTGCTTCT TTGTATAACC 
CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GSDME ( XM_024446670.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024302438.1 Homo sapiens gasdermin E (GSDME), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu25942
Clone ID Related Accession (Same CDS sequence)	NM_004403.3 , XM_024446670.1 , NM_004403.2 , NM_001127453.1 , NM_001127453.2
Accession Version	XM_024446670.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	gasdermin-E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG 
AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT 
GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC 
GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG 
CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG 
CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA 
AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG 
AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT 
GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT 
GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT 
GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG 
CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT 
CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA 
CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG 
GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT 
GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC 
ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG 
CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG 
CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA 
GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT 
CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA 
GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG 
AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT 
CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024302438.1
CDS	236..1726
Translation	MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSPVVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCALGREHS

Target ORF information:

RefSeq Version	XM_024446670.1
Organism	Homo sapiens(human)
Definition	Homo sapiens gasdermin E (GSDME), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446670.1

ORF Insert Sequence:

ATGTTTGCCA AAGCAACCAG GAATTTTCTT AGAGAAGTTG ATGCTGATGG TGACCTGATT 
GCAGTATCAA ATCTGAATGA CTCTGATAAG TTACAGCTTC TAAGTCTGGT GACAAAAAAG 
AAGAGATTCT GGTGCTGGCA GAGACCCAAG TACCAGTTTT TATCCCTCAC CCTTGGCGAT 
GTACTCATAG AAGACCAATT TCCGAGTCCA GTGGTCGTGG AGTCGGACTT TGTGAAATAC 
GAGGGCAAGT TTGCAAACCA CGTGAGTGGA ACCCTGGAGA CTGCACTGGG GAAGGTCAAG 
CTGAACCTGG GGGGCAGCAG CCGCGTAGAG AGCCAGTCTT CATTTGGAAC CCTGAGGAAG 
CAGGAGGTGG ATTTGCAGCA GCTCATCAGA GACTCTGCCG AGAGAACAAT AAATCTGAGA 
AACCCTGTGC TCCAGCAGGT GCTGGAAGGA AGGAATGAGG TCCTGTGCGT TTTGACACAG 
AAGATCACGA CGATGCAGAA GTGTGTGATC TCTGAGCACA TGCAGGTCGA GGAGAAGTGT 
GGTGGCATCG TGGGCATCCA GACCAAGACG GTGCAGGTGT CAGCGACGGA GGATGGGAAT 
GTCACCAAGG ACTCCAACGT GGTGCTGGAG ATCCCAGCTG CCACCACCAT TGCCTACGGT 
GTCATTGAGT TATACGTGAA ACTGGACGGC CAGTTCGAGT TCTGCCTTCT CCGAGGGAAG 
CAAGGTGGCT TCGAGAACAA GAAGAGAATT GACTCTGTCT ACCTGGACCC CCTGGTCTTT 
CGAGAGTTTG CATTCATAGA CATGCCAGAT GCTGCGCATG GGATATCTTC CCAGGATGGA 
CCATTAAGTG TTTTAAAGCA AGCGACCCTG CTCCTGGAGA GGAATTTCCA TCCATTTGCG 
GAGCTGCCTG AGCCACAACA GACAGCTTTG AGTGACATCT TCCAGGCGGT CCTATTTGAT 
GATGAACTAC TCATGGTCCT GGAACCAGTG TGCGATGACC TGGTCAGCGG CCTCTCGCCC 
ACAGTGGCGG TGCTGGGGGA GCTGAAGCCC CGGCAGCAGC AGGACCTTGT GGCCTTCCTG 
CAGCTGGTGG GGTGCAGCTT ACAGGGTGGG TGTCCGGGCC CCGAGGATGC AGGCAGCAAG 
CAGCTGTTTA TGACAGCCTA CTTCTTGGTC AGTGCCCTCG CAGAAATGCC AGATAGCGCA 
GCAGCTCTGC TGGGCACTTG CTGCAAACTC CAGATCATTC CCACACTGTG CCACTTGCTT 
CGTGCTCTGT CTGATGATGG AGTATCTGAT CTTGAAGACC CAACCTTGAC TCCCCTGAAA 
GATACAGAAA GGTTTGGGAT TGTGCAGCGC TTGTTTGCCT CAGCTGACAT TAGTCTGGAG 
AGACTGAAGT CATCTGTGAA AGCTGTCATT CTGAAGGACT CTAAAGTCTT CCCACTGCTT 
CTTTGTATAA CCCTGAATGG ACTCTGTGCT TTAGGCAGAG AACATTCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GSDME ( XM_017011802.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867291.1 Homo sapiens gasdermin E (GSDME), transcript variant X2, mRNA.

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CloneID	OHu26331
Clone ID Related Accession (Same CDS sequence)	XM_017011802.1 , NM_001127454.1 , NM_001127454.2
Accession Version	XM_017011802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	gasdermin-E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGT GTGTGATCTC TGAGCACATG CAGGTCGAGG AGAAGTGTGG TGGCATCGTG 
GGCATCCAGA CCAAGACGGT GCAGGTGTCA GCGACGGAGG ATGGGAATGT CACCAAGGAC 
TCCAACGTGG TGCTGGAGAT CCCAGCTGCC ACCACCATTG CCTACGGTGT CATTGAGTTA 
TACGTGAAAC TGGACGGCCA GTTCGAGTTC TGCCTTCTCC GAGGGAAGCA AGGTGGCTTC 
GAGAACAAGA AGAGAATTGA CTCTGTCTAC CTGGACCCCC TGGTCTTTCG AGAGTTTGCA 
TTCATAGACA TGCCAGATGC TGCGCATGGG ATATCTTCCC AGGATGGACC ATTAAGTGTT 
TTAAAGCAAG CGACCCTGCT CCTGGAGAGG AATTTCCATC CATTTGCGGA GCTGCCTGAG 
CCACAACAGA CAGCTTTGAG TGACATCTTC CAGGCGGTCC TATTTGATGA TGAACTACTC 
ATGGTCCTGG AACCAGTGTG CGATGACCTG GTCAGCGGCC TCTCGCCCAC AGTGGCGGTG 
CTGGGGGAGC TGAAGCCCCG GCAGCAGCAG GACCTTGTGG CCTTCCTGCA GCTGGTGGGG 
TGCAGCTTAC AGGGTGGGTG TCCGGGCCCC GAGGATGCAG GCAGCAAGCA GCTGTTTATG 
ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG ATAGCGCAGC AGCTCTGCTG 
GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC ACTTGCTTCG TGCTCTGTCT 
GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC CCCTGAAAGA TACAGAAAGG 
TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA GTCTGGAGAG ACTGAAGTCA 
TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC CACTGCTTCT TTGTATAACC 
CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867291.1
CDS	352..1350
Translation	MQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCALGREHS

Target ORF information:

RefSeq Version	XM_017011802.1
Organism	Homo sapiens(human)
Definition	Homo sapiens gasdermin E (GSDME), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011802.1

ORF Insert Sequence:

ATGCAGAAGT GTGTGATCTC TGAGCACATG CAGGTCGAGG AGAAGTGTGG TGGCATCGTG 
GGCATCCAGA CCAAGACGGT GCAGGTGTCA GCGACGGAGG ATGGGAATGT CACCAAGGAC 
TCCAACGTGG TGCTGGAGAT CCCAGCTGCC ACCACCATTG CCTACGGTGT CATTGAGTTA 
TACGTGAAAC TGGACGGCCA GTTCGAGTTC TGCCTTCTCC GAGGGAAGCA AGGTGGCTTC 
GAGAACAAGA AGAGAATTGA CTCTGTCTAC CTGGACCCCC TGGTCTTTCG AGAGTTTGCA 
TTCATAGACA TGCCAGATGC TGCGCATGGG ATATCTTCCC AGGATGGACC ATTAAGTGTT 
TTAAAGCAAG CGACCCTGCT CCTGGAGAGG AATTTCCATC CATTTGCGGA GCTGCCTGAG 
CCACAACAGA CAGCTTTGAG TGACATCTTC CAGGCGGTCC TATTTGATGA TGAACTACTC 
ATGGTCCTGG AACCAGTGTG CGATGACCTG GTCAGCGGCC TCTCGCCCAC AGTGGCGGTG 
CTGGGGGAGC TGAAGCCCCG GCAGCAGCAG GACCTTGTGG CCTTCCTGCA GCTGGTGGGG 
TGCAGCTTAC AGGGTGGGTG TCCGGGCCCC GAGGATGCAG GCAGCAAGCA GCTGTTTATG 
ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG ATAGCGCAGC AGCTCTGCTG 
GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC ACTTGCTTCG TGCTCTGTCT 
GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC CCCTGAAAGA TACAGAAAGG 
TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA GTCTGGAGAG ACTGAAGTCA 
TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC CACTGCTTCT TTGTATAACC 
CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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GSDME cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム