FREM3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FREM3
Entrez Gene ID	166752
Full Name	FRAS1 related extracellular matrix 3
General protein information	Preferred Names FRAS1 related extracellular matrix 3 Names FRAS1-related extracellular matrix protein 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q31.21
Summary	This gene encodes an integral membrane protein containing numerous CSPG (chondroitin sulfate proteoglycan element) repeats and Calx-beta domains. The protein belongs to the family of FRAS1/FREM extracellular matrix proteins and may play a role cell adhesion. [provided by RefSeq, Feb 2017].
Disorder MIM:	608946

mRNA and Protein(s)

mRNA	Protein	Name
NM_001168235.1	NP_001161707.1	FRAS1-related extracellular matrix protein 3 precursor
NM_001168235.2	NP_001161707.1	FRAS1-related extracellular matrix protein 3 precursor

Pathways from BioSystems

Homologies

	FREM3	XP_001092507.2
Rattus norvegicus (Norway rat)	Frem3	NP_001178628.1
Homo sapiens (human)	FREM3	NP_001161707.1
Pan troglodytes (chimpanzee)	FREM3	XP_517461.3
Canis lupus familiaris (dog)	FREM3	XP_005629502.1
Mus musculus (house mouse)	Frem3	NP_001161370.1
Bos taurus (cattle)	FREM3	XP_002694406.2

Gene Ontology
Bibliography

Related articles in PubMed

FRAS1-related extracellular matrix 3 (FREM3) single-nucleotide polymorphism effects on gene expression, amygdala reactivity and perceptual processing speed: An accelerated aging pathway of depression risk.
Nikolova YS, Iruku SP, Lin CW, Conley ED, Puralewski R, French B, Hariri AR, Sibille E
Frontiers in psychology61377(2015)

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
B?hm D, Keller K, Pieter J, Boehm N, Wolters D, Siggelkow W, Lebrecht A, Schmidt M, K?lbl H, Pfeiffer N, Grus FH
Oncology reports28(2)429-38(2012 Aug)

A study of the combined effects of the EHD3 and FREM3 genes in patients with major depressive disorder.
Shi C, Zhang K, Wang X, Shen Y, Xu Q
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics159B(3)336-42(2012 Apr)

Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury.
Barallobre-Barreiro J, Didangelos A, Schoendube FA, Drozdov I, Yin X, Fern?ndez-Caggiano M, Willeit P, Puntmann VO, Aldama-L?pez G, Shah AM, Dom?nech N, Mayr M
Circulation125(6)789-802(2012 Feb)

The Fras1/Frem family of extracellular matrix proteins: structure, function, and association with Fraser syndrome and the mouse bleb phenotype.
Petrou P, Makrygiannis AK, Chalepakis G
Connective tissue research49(3)277-82(2008)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The combined effect of the EHD3 and FREM3 genes may play an important role in developing major depressive disorder.
Title: A study of the combined effects of the EHD3 and FREM3 genes in patients with major depressive disorder.

The following FREM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23203	NM_001168235.2 Latest version!	Homo sapiens FRAS1 related extracellular matrix 3 (FREM3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
View Old Accession Versions >>
OHu23203	NM_001168235.1	Homo sapiens FRAS1 related extracellular matrix 3 (FREM3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FREM3 ( NM_001168235.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001161707.1 Homo sapiens FRAS1 related extracellular matrix 3 (FREM3), mRNA.

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CloneID	OHu23203
Clone ID Related Accession (Same CDS sequence)	NM_001168235.1 , NM_001168235.2
Accession Version	NM_001168235.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC139713.1, AW953229.1, DN831271.1, AI184955.1, DR731220.1, DR007885.1, BE223071.1 and R44783.1. This sequence is a reference standard in the RefSeqGene project. On or before Dec 5, 2009 this sequence version replaced XM_001127662.2, XM_001720656.1, XM_094074.10. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.105583.1, SRR1803611.254327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGG CTTCTCGGCA CCCGACTGGG ACGCCCCGGC AGCTCCTTGT GGCGCTCGCC 
TGCCTGCTCT TGAGTCGCCC CGCGCTGCAG GGACGGGCAT CCTCACTTGG GACCGAGCCC 
GACCCGGCGC TTTACCTGCC CGCCCGGGGT GCGCTTGACG GCACTCGCCC CGACGGCCCC 
AGCGTGCTGA TTGCCAACCC TGGACTCCGG GTGCCCCTGG GTCGTTCCCT TTGGCTCGAC 
CCGCTCCGGG ATCTGGTGAT TGGAGTGCAG CCGGGGGACC GGTGCGAAGT CACGGTACTG 
GACGCCCTGC CGCGGCTCAA GGGCGCGCTC TCCCCGCGCC GCTTCCCCTG CACCTTCGGG 
CCCCGCCAAG TCCAGTACAC TCACTTCGGC TCCCACAGCC CCGGACGCGC CCGGGTGCTG 
CTGCAGCTGC GCTACGACGC CCCGACTCAC ACTCTGGTGC TGCCCTTCAC GCTGGCGGTG 
GACTTGGTCT TCTCCCAGCT GGAGCTGGTG ACGCGTAACA GGCCTTTGGT AGTGGAGAAG 
CTGCGAAGCT GGAGCCGCGC CATAGACAGG AGAGTGCTGG ACTTCGCCTC CCTGAAGTCT 
GGAGCCACGG CCACCCGCAG GTGCCGGCTT ACCCCACTTC CTCACGAGGA CGGCCCCCTG 
CCCAAGTACG GGCGCTTGGT GGACGCGGTG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTCCTCCG TGCTGGGGTG CGCTATCAGC ACACAGCCAC CTCCTCGCCC 
AACCGTGACT ACGTGCCCAT GATGGTGGAG CTGCTGGGGC CTGAGGGCCA AGACGCTGGG 
TCCGCGGGTG TGCTGGTCCG CGAGCACTTC CAGCTGCTCG TGAGGATCCG CGGCGGAGCC 
GAGAACACAC CGCCCAGGCC CAGCTTCATG GCCACGATGA TGATGGAGGT GGATCCACTG 
GTGCTGACAG CCCTGACGCC TGACGCACTG GCCGCGGAGG ACGTCGAGTC AGACCCTGGT 
GACCTGGTGT TCAACATTCT GAACGCCCCC ACTCACCCAC CAGGGCACCC GGGGCAACAG 
GGCTACGTGG TCAGCACCGA CGACCCTCTA GGGCTTCCAG TCTCCTTCTT CACCCAGCAG 
GAGCTGAGGG AGCTGAAGAT TGCCTATCAG CCCCCTGCAG AGAACTCCCA TGGGGAGCGC 
CTCTTTCAGC TGGAGCTGGA GGTGGTGGAC GGAGACGGCG CCGCCTCAGA CCCCTTTGCC 
TTCATGGTGA CAGTGAAATC CATGAATACT CTGGTCCCAG TGGCTAGCCA TAACAGGGGA 
CTTGTGCTTT TTGAAGGTCA GTCAAGGCCC TTGTCCAGCA CCCACAGCAT TCCTATCAGT 
GATAAAGATA ACCTGGAAGA GGTGAAAATG GCTGCAGTCA GGGGCTTGAG ACATGGGCAG 
CTGGTGGTGT TTGGGGCACC TGCTGGGTGC AAGTATTTCA CACCAGCGGA CCTGGCAGCA 
GGGCGAGTGG TGTATCAGCA TGATGGCAGC AACACCTACA GTGACAATAT CATCTTCCGG 
ATGGAGGACG GGCACCACCA AGTGGACTTC CTCTTTCCCC TCACCATCCT ACCTGTGGAT 
GATGAACCAC CAATGGTCAA TACTAACACA GGACTCTCAC TCACTGAAGG GCAGGTGGTC 
CAGATCTCTC CCTTTGTACT GAGTGCTACT GATATTGACT CTGAGGACTC AACCATCCAC 
TTTGTGCTGG AGAACCAGCC CCTAAAAGGA AATGAGGAAG AGCCTCAGTG GGAGTTGGCC 
CCTGGTTCCT CCCACTCAGG CCACTACCTG GGGGACCTGT TGCTGCAGCA GGCTGAACTA 
CCTCTCTCAA CTGAAGATGA AGACTGGCAC TACATGGAAA AGGAAGGGCT TTATGAGAAA 
GTGGTGACTG AGTGGCTACA GAGAGACATA ATGGAAGGGA GACTCTTCTA CCGCCATCTT 
GGACCACACA GCCCTCAATC TGTAATGGTC CAGCTGGCTT TCCATGTGCA GGATGACCAT 
GACCCACCCA ATCTCTCCAA ACAGCACATT TTCACCATCA AGGTCCAACC AGTGGATATA 
CTGAGTCCAC AGCTGTATCC AGGAACTACA CTAGAAATGA CTGTACAAGA ATACCAACTC 
ACTCACTTCC AGAAGAATTT CCTCCGATAC ATTGACCAGG ACTCTGATGA CCAGAACCTA 
TGGTACACCC TACTGACACT CCCGACTGAC ACAGATGGCA ACCACCAAGT CCGGGCTGGA 
GAAATTGTGC TCACTGATTC ACCAGACACA CTCATCATGC ACTTTACCCA AGCCCAGGTA 
AATCAGCATA AAGTTGCCTA CCAGCCTCCA CAGAAATTGG GTATTGCACC ACGGGTTGTG 
CAGTTTACTT ACCAGGTGGA AGATGCTGCA GGCAATAGCG TGCCAGGCAC ATTCACATTA 
TTCCTGCAAC CTGTGGACAA CCAGCCCCCA GAAGTCACCA ACAGAGGCTT TGCTATCTTA 
GAGGGAGGCA GCTTTAACCT CAGCAGTAAT GAGCTGCATG TTACAGACCC AGACACAGAC 
ATTGACCAAA TTGTCTTCAT ATTAGTCCGG GGTCCCCAAC ATGGACACTT GCAGTACTTT 
AAAAGATGTA TGGTCCCAGG GGAATCTTTC ATGCAAGCTG ATGTTATTAA CGGGAGTGTC 
TCTTACCAGC ATGGCAGAGA CCAGACGACT ACCAGTGACA CTTTCCATCT GGAAGTAAGT 
GATGGGGTGC ACCATATACC CATCACCATC CCAATTTCTG TGCATCCCAA TGTGGCTAAC 
AGAAGTCCTA GGATCTCTCT CAGAAGTAGT TCATTATTGG ATGTTTCTAT AGACGTACTA 
GAGAATAAAG CCACTGAAAT TACCATGGGT GTCATCCATG GCAAAAGGAA GGATGTAGGT 
GACTTGATGC TGTCTTTCAT TGTAAAGGAC AGCCCCAAAC TGGGCACTAT TCTGGTAAAT 
GGTTTGCCCA CAGAACGATT CACCCAAGAG GATCTCATCA ATGGGAGAGT AGCCTATGCC 
CACACTGCAG GTGAAGTTGG TTTTCAAAAG CAGCACGATG CCTTCAGCCT CATCCTCTCC 
AAAGATTCTT ACCAATGGGT AGTGGGGAAT AGCATAATAG AGAAAGTACA GGTACAAGTA 
ACTGTGCTGC CTGTGGACAA TGTGGGACCC AAGGTCTTCG TCGGGGAGTC CTTCATTGTC 
TATGAAGGTG AGAAGAACTC CTTGACCTTA CAACATCTCC ATGTTGAAGA TGTGGACACT 
CATCAAGATG AACTCCTCTG CACAGTGACT AGTCAGCCAG CCTCTGGCTA CCTGGAAAAG 
ATTGCATCAG CTCCTGGTTC AAAAATGTCC CAGTCTGGTA GCCCTATCAG TGCTTTCTCC 
CTCAGAGATA TCCAAGTGAG GCATATCAAT TATGTACAGA GTATTCACAA GGGAGTAGAG 
CCACAAGAGG ACCAATTCAC CTTTTATTGC TCTGATGGCA TCAACTTCTC CCCAAATGTC 
TTCTTCCCTA TAATCATCCT ACCCACCAAT GATGAGCAGC CTAAACTTTT TGCCCATGAG 
TTTAAGGTAC TAGAGGGGAT GAGCCTGGTC ATAGACACCC AGCTGCTTAA TGGAGCAGAT 
GCTGACCTGC CACCAAATGA GCTCCACTTT CAACTCACAG CCCTCCCTCG GCATGGACGA 
ATCATACAGC AGCTGGCTAC AGGCAGCCAG CCCATCCACA GCTTCACCCT CAAGGAGATC 
CAGGAGGCCT CCACCATTGT GTATGAGCAT GATGACTCAG AGACAAAAGA GGACAGTTTT 
GAGGTCTGGC TGAGTGACGG CAAGCACACA ACCCACAGGA AGGTACCCAT TGTAGTGACC 
CTAGTGGATG ATGAAACTCC TCACCTGACT GTCAACAATG GGCTGAAGGT AGAGAAAGGA 
CACTCTGAGA TCATCACAAA TCGGATCCTC AAGGCCACAG ATCTTGACTC AGATGATAAA 
AGCCTCAGTT TTGTCCTCCA TTCTGGGCCT CAACAAGGGC TTCTACAGAG GCTGAGAAAA 
CCCAGAGGAG AAGTGAGGAA CAATCTTACT CTGGGAATGA ACTTTACCCA GGATGAGATT 
AACAGAGGCC TCATCTGCTA TATCCACACA GGCCAAGAAG GGATTGTTGA CATTATCAAG 
TTTGATGTTA CTGATGGGGT TAACACTTTG ACAGACCACT ACTTCTATGT CACCATTGGC 
AACTTAGACA GCGTCTTCCC TGAGGTAATC AGCAAAAGGA TCACCTTGAT AGAAGGTGCC 
AGAGTGACCC TTACCAACAA TCTGCTTACC AACAGTGACA TCAACAGCTC TGATGAACAT 
CACTTTAGCA TTACACGGGC TCCAAGCCTG GGTCACTTAG AAAGTTCTGA CTATGCTGGG 
GAACCCATTG CCTCTTTCAC TCAACTTCAA TTGGCTAGCA ACAAAATATC CTATGTCCAT 
ACTTCAAATG ATGAGAAAAA GATGGACAGC TTCGAATTTC AAGTGATCGG CGAACTCTAC 
CCTGTGTTCA GAACCTTCAG GATCTTCATC ACTGATGTGG ATAATAAGAA ACCTATCTTG 
ACCATCCATA GACTAACACT ACAGAAAGAG GATAGCCAAC TGATCACTCT CCTTGAGTTG 
ACAGTGGAAG ACAGTGATAC CCCAGATGAC CTTATTCTCT TTACCATCAC CCAGGTCCCC 
ATGCATGGCA AGATTTTGTA CAATGGTAGC CGTCCCGTGA CCACTTTCAC CAAGCAAGAC 
CTGAACAAGA ACCTGATTAG CTACAAGCAT GACGGCAGTG AGACCACTGA AGATAGTTTC 
TCCTTGACTG TGACAGACGG CACTCACACT GATTTCTATG TCCTACCAGA CACTGCCCTG 
GCGACACACA AACCTCAAGT AATGAGGGTC CAGATAAGAT CATTAGACAA TAGGCTTCCC 
CAGATTACTA CCAACAGGGG TGCCCCAGCC TTGAAGCGCC TTCACACTGG ACACATGGGC 
TTCCTGATTA CCAGCAAGTC TCTGAAGGCA GAAGATCAGG ACAGTCCCCA CAGGCTTCTG 
AAGTATAAAG TGACCAGAGG ACCAGAGCAT GGCTTCATTA TCAAAACTGG CCTTGGAAAC 
CAGAGCACTC GAGTGTTTAC ACAAGCTGAC ATTGATGAGA TGAAGATATC CTATGTCTTG 
AATGAGGGCA GCAACGCATC AAAGGACATC TTCTATTTCT CTGTTGAAGA CAATGGAGGA 
AATAAACTAA CAAATCAGCC TTTCCATCTA AACTGGGCTT GGATTTGTTT GGAGAAAGAG 
TACTACATTG TGGATGAAGA TTCCACATTC TTAGAAGTGA CCCTTACACG CAGAGGATAC 
CTTGGAGAAA CATCATTTAT CAGCATTGGT ACCAAAGATG AAACTGCAAA AAAAGACAAA 
GATTTCAAAT GGAAGACCAA TAAACAGATC CAGTTCAATC CTGGACAGAC TACAGCCACA 
TGGAGGGTGA GAATTATACC TGACAATGAA TATGAGACTT CAGAGACCTT CCAGATCATT 
CTGTCTGAAC CTCTCATGGC TGTACTGGAG TTTCCAGAAA TGGCAACGGT TGAAATTGTA 
GACCCTGGAG ATGAATCAAC AGTTTATATT CCAGAGGCGG AATACAAAAT AGAAGAGGAC 
ATTGGGGAAC TTTTGATTCC AGTAAGACGA TCTGGAGATG CAAGCCAGGA ACTAATCGTC 
ATTTGCTCCA CACGTCAAGG TTCTGCCACA GGCACGATTT CTTCCACAGT GTTATTCTCT 
GATTACATCT CACGTCCAGA AGACCACACC AGCATCCTCC ACTTTGACAA GAATGAGACA 
CAGAAGACCT GCCAGGTCCT GATCATTGAT GACTCCCTTT ATGAAGAGGA GGAATCCTTC 
AGCGTTTCAC TTAGGCTGCC AGTGGGAGGA CAGCTGGGAG CCAGATTCCC CACCACTAAG 
GTGACTATTC TGGCTGATCG TTATGATGAA CCTGTCCTGC ACTTTGGGGA TGCTGAATAT 
CACGTCAATG AAAGTGCTCG CTACGTGGAG GTTTGTGTTT GGAGAAGAGG CACTGATCTT 
TCCCAACCAT CATCCATCGC CGTGCGCTCC AGAAAGTCAG AGCAGGAGTC AGCAGAAGCT 
GGAACAGATT ATGTTGGTAT CAGCCGAAAC CTGGACTTTG CTCCAGGCGT GCGCATGCAG 
ACATTCCAAG TGACCATCCT TGATGACCTG GGACAGCCTA CCTTGGAAGG CCCAGAGAAG 
TTTGAATTAC TTCTTCAGAT GCCTATGGGT GCAGTGCTTG GAGAACCAAA TAAAACTACC 
ATTTTCATCG AGGACACCAT CACGGACTGT AAGCAGAGTG CTTGTAGTTC CTTTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161707.1
CDS	1..6420
Translation	MAGASRHPTGTPRQLLVALACLLLSRPALQGRASSLGTEPDPALYLPARGALDGTRPDGPSVLIANPGLRVPLGRSLWLDPLRDLVIGVQPGDRCEVTVLDALPRLKGALSPRRFPCTFGPRQVQYTHFGSHSPGRARVLLQLRYDAPTHTLVLPFTLAVDLVFSQLELVTRNRPLVVEKLRSWSRAIDRRVLDFASLKSGATATRRCRLTPLPHEDGPLPKYGRLVDAVGAPLPRGKGVDCEAFLRAGVRYQHTATSSPNRDYVPMMVELLGPEGQDAGSAGVLVREHFQLLVRIRGGAENTPPRPSFMATMMMEVDPLVLTALTPDALAAEDVESDPGDLVFNILNAPTHPPGHPGQQGYVVSTDDPLGLPVSFFTQQELRELKIAYQPPAENSHGERLFQLELEVVDGDGAASDPFAFMVTVKSMNTLVPVASHNRGLVLFEGQSRPLSSTHSIPISDKDNLEEVKMAAVRGLRHGQLVVFGAPAGCKYFTPADLAAGRVVYQHDGSNTYSDNIIFRMEDGHHQVDFLFPLTILPVDDEPPMVNTNTGLSLTEGQVVQISPFVLSATDIDSEDSTIHFVLENQPLKGNEEEPQWELAPGSSHSGHYLGDLLLQQAELPLSTEDEDWHYMEKEGLYEKVVTEWLQRDIMEGRLFYRHLGPHSPQSVMVQLAFHVQDDHDPPNLSKQHIFTIKVQPVDILSPQLYPGTTLEMTVQEYQLTHFQKNFLRYIDQDSDDQNLWYTLLTLPTDTDGNHQVRAGEIVLTDSPDTLIMHFTQAQVNQHKVAYQPPQKLGIAPRVVQFTYQVEDAAGNSVPGTFTLFLQPVDNQPPEVTNRGFAILEGGSFNLSSNELHVTDPDTDIDQIVFILVRGPQHGHLQYFKRCMVPGESFMQADVINGSVSYQHGRDQTTTSDTFHLEVSDGVHHIPITIPISVHPNVANRSPRISLRSSSLLDVSIDVLENKATEITMGVIHGKRKDVGDLMLSFIVKDSPKLGTILVNGLPTERFTQEDLINGRVAYAHTAGEVGFQKQHDAFSLILSKDSYQWVVGNSIIEKVQVQVTVLPVDNVGPKVFVGESFIVYEGEKNSLTLQHLHVEDVDTHQDELLCTVTSQPASGYLEKIASAPGSKMSQSGSPISAFSLRDIQVRHINYVQSIHKGVEPQEDQFTFYCSDGINFSPNVFFPIIILPTNDEQPKLFAHEFKVLEGMSLVIDTQLLNGADADLPPNELHFQLTALPRHGRIIQQLATGSQPIHSFTLKEIQEASTIVYEHDDSETKEDSFEVWLSDGKHTTHRKVPIVVTLVDDETPHLTVNNGLKVEKGHSEIITNRILKATDLDSDDKSLSFVLHSGPQQGLLQRLRKPRGEVRNNLTLGMNFTQDEINRGLICYIHTGQEGIVDIIKFDVTDGVNTLTDHYFYVTIGNLDSVFPEVISKRITLIEGARVTLTNNLLTNSDINSSDEHHFSITRAPSLGHLESSDYAGEPIASFTQLQLASNKISYVHTSNDEKKMDSFEFQVIGELYPVFRTFRIFITDVDNKKPILTIHRLTLQKEDSQLITLLELTVEDSDTPDDLILFTITQVPMHGKILYNGSRPVTTFTKQDLNKNLISYKHDGSETTEDSFSLTVTDGTHTDFYVLPDTALATHKPQVMRVQIRSLDNRLPQITTNRGAPALKRLHTGHMGFLITSKSLKAEDQDSPHRLLKYKVTRGPEHGFIIKTGLGNQSTRVFTQADIDEMKISYVLNEGSNASKDIFYFSVEDNGGNKLTNQPFHLNWAWICLEKEYYIVDEDSTFLEVTLTRRGYLGETSFISIGTKDETAKKDKDFKWKTNKQIQFNPGQTTATWRVRIIPDNEYETSETFQIILSEPLMAVLEFPEMATVEIVDPGDESTVYIPEAEYKIEEDIGELLIPVRRSGDASQELIVICSTRQGSATGTISSTVLFSDYISRPEDHTSILHFDKNETQKTCQVLIIDDSLYEEEESFSVSLRLPVGGQLGARFPTTKVTILADRYDEPVLHFGDAEYHVNESARYVEVCVWRRGTDLSQPSSIAVRSRKSEQESAEAGTDYVGISRNLDFAPGVRMQTFQVTILDDLGQPTLEGPEKFELLLQMPMGAVLGEPNKTTIFIEDTITDCKQSACSSFD

Target ORF information:

RefSeq Version	NM_001168235.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 3 (FREM3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168235.1

ORF Insert Sequence:

ATGGCGGGGG CTTCTCGGCA CCCGACTGGG ACGCCCCGGC AGCTCCTTGT GGCGCTCGCC 
TGCCTGCTCT TGAGTCGCCC CGCGCTGCAG GGACGGGCAT CCTCACTTGG GACCGAGCCC 
GACCCGGCGC TTTACCTGCC CGCCCGGGGT GCGCTTGACG GCACTCGCCC CGACGGCCCC 
AGCGTGCTGA TTGCCAACCC TGGACTCCGG GTGCCCCTGG GTCGTTCCCT TTGGCTCGAC 
CCGCTCCGGG ATCTGGTGAT TGGAGTGCAG CCGGGGGACC GGTGCGAAGT CACGGTACTG 
GACGCCCTGC CGCGGCTCAA GGGCGCGCTC TCCCCGCGCC GCTTCCCCTG CACCTTCGGG 
CCCCGCCAAG TCCAGTACAC TCACTTCGGC TCCCACAGCC CCGGACGCGC CCGGGTGCTG 
CTGCAGCTGC GCTACGACGC CCCGACTCAC ACTCTGGTGC TGCCCTTCAC GCTGGCGGTG 
GACTTGGTCT TCTCCCAGCT GGAGCTGGTG ACGCGTAACA GGCCTTTGGT AGTGGAGAAG 
CTGCGAAGCT GGAGCCGCGC CATAGACAGG AGAGTGCTGG ACTTCGCCTC CCTGAAGTCT 
GGAGCCACGG CCACCCGCAG GTGCCGGCTT ACCCCACTTC CTCACGAGGA CGGCCCCCTG 
CCCAAGTACG GGCGCTTGGT GGACGCGGTG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTCCTCCG TGCTGGGGTG CGCTATCAGC ACACAGCCAC CTCCTCGCCC 
AACCGTGACT ACGTGCCCAT GATGGTGGAG CTGCTGGGGC CTGAGGGCCA AGACGCTGGG 
TCCGCGGGTG TGCTGGTCCG CGAGCACTTC CAGCTGCTCG TGAGGATCCG CGGCGGAGCC 
GAGAACACAC CGCCCAGGCC CAGCTTCATG GCCACGATGA TGATGGAGGT GGATCCACTG 
GTGCTGACAG CCCTGACGCC TGACGCACTG GCCGCGGAGG ACGTCGAGTC AGACCCTGGT 
GACCTGGTGT TCAACATTCT GAACGCCCCC ACTCACCCAC CAGGGCACCC GGGGCAACAG 
GGCTACGTGG TCAGCACCGA CGACCCTCTA GGGCTTCCAG TCTCCTTCTT CACCCAGCAG 
GAGCTGAGGG AGCTGAAGAT TGCCTATCAG CCCCCTGCAG AGAACTCCCA TGGGGAGCGC 
CTCTTTCAGC TGGAGCTGGA GGTGGTGGAC GGAGACGGCG CCGCCTCAGA CCCCTTTGCC 
TTCATGGTGA CAGTGAAATC CATGAATACT CTGGTCCCAG TGGCTAGCCA TAACAGGGGA 
CTTGTGCTTT TTGAAGGTCA GTCAAGGCCC TTGTCCAGCA CCCACAGCAT TCCTATCAGT 
GATAAAGATA ACCTGGAAGA GGTGAAAATG GCTGCAGTCA GGGGCTTGAG ACATGGGCAG 
CTGGTGGTGT TTGGGGCACC TGCTGGGTGC AAGTATTTCA CACCAGCGGA CCTGGCAGCA 
GGGCGAGTGG TGTATCAGCA TGATGGCAGC AACACCTACA GTGACAATAT CATCTTCCGG 
ATGGAGGACG GGCACCACCA AGTGGACTTC CTCTTTCCCC TCACCATCCT ACCTGTGGAT 
GATGAACCAC CAATGGTCAA TACTAACACA GGACTCTCAC TCACTGAAGG GCAGGTGGTC 
CAGATCTCTC CCTTTGTACT GAGTGCTACT GATATTGACT CTGAGGACTC AACCATCCAC 
TTTGTGCTGG AGAACCAGCC CCTAAAAGGA AATGAGGAAG AGCCTCAGTG GGAGTTGGCC 
CCTGGTTCCT CCCACTCAGG CCACTACCTG GGGGACCTGT TGCTGCAGCA GGCTGAACTA 
CCTCTCTCAA CTGAAGATGA AGACTGGCAC TACATGGAAA AGGAAGGGCT TTATGAGAAA 
GTGGTGACTG AGTGGCTACA GAGAGACATA ATGGAAGGGA GACTCTTCTA CCGCCATCTT 
GGACCACACA GCCCTCAATC TGTAATGGTC CAGCTGGCTT TCCATGTGCA GGATGACCAT 
GACCCACCCA ATCTCTCCAA ACAGCACATT TTCACCATCA AGGTCCAACC AGTGGATATA 
CTGAGTCCAC AGCTGTATCC AGGAACTACA CTAGAAATGA CTGTACAAGA ATACCAACTC 
ACTCACTTCC AGAAGAATTT CCTCCGATAC ATTGACCAGG ACTCTGATGA CCAGAACCTA 
TGGTACACCC TACTGACACT CCCGACTGAC ACAGATGGCA ACCACCAAGT CCGGGCTGGA 
GAAATTGTGC TCACTGATTC ACCAGACACA CTCATCATGC ACTTTACCCA AGCCCAGGTA 
AATCAGCATA AAGTTGCCTA CCAGCCTCCA CAGAAATTGG GTATTGCACC ACGGGTTGTG 
CAGTTTACTT ACCAGGTGGA AGATGCTGCA GGCAATAGCG TGCCAGGCAC ATTCACATTA 
TTCCTGCAAC CTGTGGACAA CCAGCCCCCA GAAGTCACCA ACAGAGGCTT TGCTATCTTA 
GAGGGAGGCA GCTTTAACCT CAGCAGTAAT GAGCTGCATG TTACAGACCC AGACACAGAC 
ATTGACCAAA TTGTCTTCAT ATTAGTCCGG GGTCCCCAAC ATGGACACTT GCAGTACTTT 
AAAAGATGTA TGGTCCCAGG GGAATCTTTC ATGCAAGCTG ATGTTATTAA CGGGAGTGTC 
TCTTACCAGC ATGGCAGAGA CCAGACGACT ACCAGTGACA CTTTCCATCT GGAAGTAAGT 
GATGGGGTGC ACCATATACC CATCACCATC CCAATTTCTG TGCATCCCAA TGTGGCTAAC 
AGAAGTCCTA GGATCTCTCT CAGAAGTAGT TCATTATTGG ATGTTTCTAT AGACGTACTA 
GAGAATAAAG CCACTGAAAT TACCATGGGT GTCATCCATG GCAAAAGGAA GGATGTAGGT 
GACTTGATGC TGTCTTTCAT TGTAAAGGAC AGCCCCAAAC TGGGCACTAT TCTGGTAAAT 
GGTTTGCCCA CAGAACGATT CACCCAAGAG GATCTCATCA ATGGGAGAGT AGCCTATGCC 
CACACTGCAG GTGAAGTTGG TTTTCAAAAG CAGCACGATG CCTTCAGCCT CATCCTCTCC 
AAAGATTCTT ACCAATGGGT AGTGGGGAAT AGCATAATAG AGAAAGTACA GGTACAAGTA 
ACTGTGCTGC CTGTGGACAA TGTGGGACCC AAGGTCTTCG TCGGGGAGTC CTTCATTGTC 
TATGAAGGTG AGAAGAACTC CTTGACCTTA CAACATCTCC ATGTTGAAGA TGTGGACACT 
CATCAAGATG AACTCCTCTG CACAGTGACT AGTCAGCCAG CCTCTGGCTA CCTGGAAAAG 
ATTGCATCAG CTCCTGGTTC AAAAATGTCC CAGTCTGGTA GCCCTATCAG TGCTTTCTCC 
CTCAGAGATA TCCAAGTGAG GCATATCAAT TATGTACAGA GTATTCACAA GGGAGTAGAG 
CCACAAGAGG ACCAATTCAC CTTTTATTGC TCTGATGGCA TCAACTTCTC CCCAAATGTC 
TTCTTCCCTA TAATCATCCT ACCCACCAAT GATGAGCAGC CTAAACTTTT TGCCCATGAG 
TTTAAGGTAC TAGAGGGGAT GAGCCTGGTC ATAGACACCC AGCTGCTTAA TGGAGCAGAT 
GCTGACCTGC CACCAAATGA GCTCCACTTT CAACTCACAG CCCTCCCTCG GCATGGACGA 
ATCATACAGC AGCTGGCTAC AGGCAGCCAG CCCATCCACA GCTTCACCCT CAAGGAGATC 
CAGGAGGCCT CCACCATTGT GTATGAGCAT GATGACTCAG AGACAAAAGA GGACAGTTTT 
GAGGTCTGGC TGAGTGACGG CAAGCACACA ACCCACAGGA AGGTACCCAT TGTAGTGACC 
CTAGTGGATG ATGAAACTCC TCACCTGACT GTCAACAATG GGCTGAAGGT AGAGAAAGGA 
CACTCTGAGA TCATCACAAA TCGGATCCTC AAGGCCACAG ATCTTGACTC AGATGATAAA 
AGCCTCAGTT TTGTCCTCCA TTCTGGGCCT CAACAAGGGC TTCTACAGAG GCTGAGAAAA 
CCCAGAGGAG AAGTGAGGAA CAATCTTACT CTGGGAATGA ACTTTACCCA GGATGAGATT 
AACAGAGGCC TCATCTGCTA TATCCACACA GGCCAAGAAG GGATTGTTGA CATTATCAAG 
TTTGATGTTA CTGATGGGGT TAACACTTTG ACAGACCACT ACTTCTATGT CACCATTGGC 
AACTTAGACA GCGTCTTCCC TGAGGTAATC AGCAAAAGGA TCACCTTGAT AGAAGGTGCC 
AGAGTGACCC TTACCAACAA TCTGCTTACC AACAGTGACA TCAACAGCTC TGATGAACAT 
CACTTTAGCA TTACACGGGC TCCAAGCCTG GGTCACTTAG AAAGTTCTGA CTATGCTGGG 
GAACCCATTG CCTCTTTCAC TCAACTTCAA TTGGCTAGCA ACAAAATATC CTATGTCCAT 
ACTTCAAATG ATGAGAAAAA GATGGACAGC TTCGAATTTC AAGTGATCGG CGAACTCTAC 
CCTGTGTTCA GAACCTTCAG GATCTTCATC ACTGATGTGG ATAATAAGAA ACCTATCTTG 
ACCATCCATA GACTAACACT ACAGAAAGAG GATAGCCAAC TGATCACTCT CCTTGAGTTG 
ACAGTGGAAG ACAGTGATAC CCCAGATGAC CTTATTCTCT TTACCATCAC CCAGGTCCCC 
ATGCATGGCA AGATTTTGTA CAATGGTAGC CGTCCCGTGA CCACTTTCAC CAAGCAAGAC 
CTGAACAAGA ACCTGATTAG CTACAAGCAT GACGGCAGTG AGACCACTGA AGATAGTTTC 
TCCTTGACTG TGACAGACGG CACTCACACT GATTTCTATG TCCTACCAGA CACTGCCCTG 
GCGACACACA AACCTCAAGT AATGAGGGTC CAGATAAGAT CATTAGACAA TAGGCTTCCC 
CAGATTACTA CCAACAGGGG TGCCCCAGCC TTGAAGCGCC TTCACACTGG ACACATGGGC 
TTCCTGATTA CCAGCAAGTC TCTGAAGGCA GAAGATCAGG ACAGTCCCCA CAGGCTTCTG 
AAGTATAAAG TGACCAGAGG ACCAGAGCAT GGCTTCATTA TCAAAACTGG CCTTGGAAAC 
CAGAGCACTC GAGTGTTTAC ACAAGCTGAC ATTGATGAGA TGAAGATATC CTATGTCTTG 
AATGAGGGCA GCAACGCATC AAAGGACATC TTCTATTTCT CTGTTGAAGA CAATGGAGGA 
AATAAACTAA CAAATCAGCC TTTCCATCTA AACTGGGCTT GGATTTGTTT GGAGAAAGAG 
TACTACATTG TGGATGAAGA TTCCACATTC TTAGAAGTGA CCCTTACACG CAGAGGATAC 
CTTGGAGAAA CATCATTTAT CAGCATTGGT ACCAAAGATG AAACTGCAAA AAAAGACAAA 
GATTTCAAAT GGAAGACCAA TAAACAGATC CAGTTCAATC CTGGACAGAC TACAGCCACA 
TGGAGGGTGA GAATTATACC TGACAATGAA TATGAGACTT CAGAGACCTT CCAGATCATT 
CTGTCTGAAC CTCTCATGGC TGTACTGGAG TTTCCAGAAA TGGCAACGGT TGAAATTGTA 
GACCCTGGAG ATGAATCAAC AGTTTATATT CCAGAGGCGG AATACAAAAT AGAAGAGGAC 
ATTGGGGAAC TTTTGATTCC AGTAAGACGA TCTGGAGATG CAAGCCAGGA ACTAATCGTC 
ATTTGCTCCA CACGTCAAGG TTCTGCCACA GGCACGATTT CTTCCACAGT GTTATTCTCT 
GATTACATCT CACGTCCAGA AGACCACACC AGCATCCTCC ACTTTGACAA GAATGAGACA 
CAGAAGACCT GCCAGGTCCT GATCATTGAT GACTCCCTTT ATGAAGAGGA GGAATCCTTC 
AGCGTTTCAC TTAGGCTGCC AGTGGGAGGA CAGCTGGGAG CCAGATTCCC CACCACTAAG 
GTGACTATTC TGGCTGATCG TTATGATGAA CCTGTCCTGC ACTTTGGGGA TGCTGAATAT 
CACGTCAATG AAAGTGCTCG CTACGTGGAG GTTTGTGTTT GGAGAAGAGG CACTGATCTT 
TCCCAACCAT CATCCATCGC CGTGCGCTCC AGAAAGTCAG AGCAGGAGTC AGCAGAAGCT 
GGAACAGATT ATGTTGGTAT CAGCCGAAAC CTGGACTTTG CTCCAGGCGT GCGCATGCAG 
ACATTCCAAG TGACCATCCT TGATGACCTG GGACAGCCTA CCTTGGAAGG CCCAGAGAAG 
TTTGAATTAC TTCTTCAGAT GCCTATGGGT GCAGTGCTTG GAGAACCAAA TAAAACTACC 
ATTTTCATCG AGGACACCAT CACGGACTGT AAGCAGAGTG CTTGTAGTTC CTTTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM3 ( NM_001168235.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001161707.1 Homo sapiens FRAS1 related extracellular matrix 3 (FREM3), mRNA.

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CloneID	OHu23203
Clone ID Related Accession (Same CDS sequence)	NM_001168235.1 , NM_001168235.2
Accession Version	NM_001168235.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC139713.1, AW953229.1, DN831271.1, AI184955.1, DR731220.1, DR007885.1, BE223071.1 and R44783.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_001168235.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.105583.1, SRR1803611.254327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000329798.5/ ENSP00000332886.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGG CTTCTCGGCA CCCGACTGGG ACGCCCCGGC AGCTCCTTGT GGCGCTCGCC 
TGCCTGCTCT TGAGTCGCCC CGCGCTGCAG GGACGGGCAT CCTCACTTGG GACCGAGCCC 
GACCCGGCGC TTTACCTGCC CGCCCGGGGT GCGCTTGACG GCACTCGCCC CGACGGCCCC 
AGCGTGCTGA TTGCCAACCC TGGACTCCGG GTGCCCCTGG GTCGTTCCCT TTGGCTCGAC 
CCGCTCCGGG ATCTGGTGAT TGGAGTGCAG CCGGGGGACC GGTGCGAAGT CACGGTACTG 
GACGCCCTGC CGCGGCTCAA GGGCGCGCTC TCCCCGCGCC GCTTCCCCTG CACCTTCGGG 
CCCCGCCAAG TCCAGTACAC TCACTTCGGC TCCCACAGCC CCGGACGCGC CCGGGTGCTG 
CTGCAGCTGC GCTACGACGC CCCGACTCAC ACTCTGGTGC TGCCCTTCAC GCTGGCGGTG 
GACTTGGTCT TCTCCCAGCT GGAGCTGGTG ACGCGTAACA GGCCTTTGGT AGTGGAGAAG 
CTGCGAAGCT GGAGCCGCGC CATAGACAGG AGAGTGCTGG ACTTCGCCTC CCTGAAGTCT 
GGAGCCACGG CCACCCGCAG GTGCCGGCTT ACCCCACTTC CTCACGAGGA CGGCCCCCTG 
CCCAAGTACG GGCGCTTGGT GGACGCGGTG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTCCTCCG TGCTGGGGTG CGCTATCAGC ACACAGCCAC CTCCTCGCCC 
AACCGTGACT ACGTGCCCAT GATGGTGGAG CTGCTGGGGC CTGAGGGCCA AGACGCTGGG 
TCCGCGGGTG TGCTGGTCCG CGAGCACTTC CAGCTGCTCG TGAGGATCCG CGGCGGAGCC 
GAGAACACAC CGCCCAGGCC CAGCTTCATG GCCACGATGA TGATGGAGGT GGATCCACTG 
GTGCTGACAG CCCTGACGCC TGACGCACTG GCCGCGGAGG ACGTCGAGTC AGACCCTGGT 
GACCTGGTGT TCAACATTCT GAACGCCCCC ACTCACCCAC CAGGGCACCC GGGGCAACAG 
GGCTACGTGG TCAGCACCGA CGACCCTCTA GGGCTTCCAG TCTCCTTCTT CACCCAGCAG 
GAGCTGAGGG AGCTGAAGAT TGCCTATCAG CCCCCTGCAG AGAACTCCCA TGGGGAGCGC 
CTCTTTCAGC TGGAGCTGGA GGTGGTGGAC GGAGACGGCG CCGCCTCAGA CCCCTTTGCC 
TTCATGGTGA CAGTGAAATC CATGAATACT CTGGTCCCAG TGGCTAGCCA TAACAGGGGA 
CTTGTGCTTT TTGAAGGTCA GTCAAGGCCC TTGTCCAGCA CCCACAGCAT TCCTATCAGT 
GATAAAGATA ACCTGGAAGA GGTGAAAATG GCTGCAGTCA GGGGCTTGAG ACATGGGCAG 
CTGGTGGTGT TTGGGGCACC TGCTGGGTGC AAGTATTTCA CACCAGCGGA CCTGGCAGCA 
GGGCGAGTGG TGTATCAGCA TGATGGCAGC AACACCTACA GTGACAATAT CATCTTCCGG 
ATGGAGGACG GGCACCACCA AGTGGACTTC CTCTTTCCCC TCACCATCCT ACCTGTGGAT 
GATGAACCAC CAATGGTCAA TACTAACACA GGACTCTCAC TCACTGAAGG GCAGGTGGTC 
CAGATCTCTC CCTTTGTACT GAGTGCTACT GATATTGACT CTGAGGACTC AACCATCCAC 
TTTGTGCTGG AGAACCAGCC CCTAAAAGGA AATGAGGAAG AGCCTCAGTG GGAGTTGGCC 
CCTGGTTCCT CCCACTCAGG CCACTACCTG GGGGACCTGT TGCTGCAGCA GGCTGAACTA 
CCTCTCTCAA CTGAAGATGA AGACTGGCAC TACATGGAAA AGGAAGGGCT TTATGAGAAA 
GTGGTGACTG AGTGGCTACA GAGAGACATA ATGGAAGGGA GACTCTTCTA CCGCCATCTT 
GGACCACACA GCCCTCAATC TGTAATGGTC CAGCTGGCTT TCCATGTGCA GGATGACCAT 
GACCCACCCA ATCTCTCCAA ACAGCACATT TTCACCATCA AGGTCCAACC AGTGGATATA 
CTGAGTCCAC AGCTGTATCC AGGAACTACA CTAGAAATGA CTGTACAAGA ATACCAACTC 
ACTCACTTCC AGAAGAATTT CCTCCGATAC ATTGACCAGG ACTCTGATGA CCAGAACCTA 
TGGTACACCC TACTGACACT CCCGACTGAC ACAGATGGCA ACCACCAAGT CCGGGCTGGA 
GAAATTGTGC TCACTGATTC ACCAGACACA CTCATCATGC ACTTTACCCA AGCCCAGGTA 
AATCAGCATA AAGTTGCCTA CCAGCCTCCA CAGAAATTGG GTATTGCACC ACGGGTTGTG 
CAGTTTACTT ACCAGGTGGA AGATGCTGCA GGCAATAGCG TGCCAGGCAC ATTCACATTA 
TTCCTGCAAC CTGTGGACAA CCAGCCCCCA GAAGTCACCA ACAGAGGCTT TGCTATCTTA 
GAGGGAGGCA GCTTTAACCT CAGCAGTAAT GAGCTGCATG TTACAGACCC AGACACAGAC 
ATTGACCAAA TTGTCTTCAT ATTAGTCCGG GGTCCCCAAC ATGGACACTT GCAGTACTTT 
AAAAGATGTA TGGTCCCAGG GGAATCTTTC ATGCAAGCTG ATGTTATTAA CGGGAGTGTC 
TCTTACCAGC ATGGCAGAGA CCAGACGACT ACCAGTGACA CTTTCCATCT GGAAGTAAGT 
GATGGGGTGC ACCATATACC CATCACCATC CCAATTTCTG TGCATCCCAA TGTGGCTAAC 
AGAAGTCCTA GGATCTCTCT CAGAAGTAGT TCATTATTGG ATGTTTCTAT AGACGTACTA 
GAGAATAAAG CCACTGAAAT TACCATGGGT GTCATCCATG GCAAAAGGAA GGATGTAGGT 
GACTTGATGC TGTCTTTCAT TGTAAAGGAC AGCCCCAAAC TGGGCACTAT TCTGGTAAAT 
GGTTTGCCCA CAGAACGATT CACCCAAGAG GATCTCATCA ATGGGAGAGT AGCCTATGCC 
CACACTGCAG GTGAAGTTGG TTTTCAAAAG CAGCACGATG CCTTCAGCCT CATCCTCTCC 
AAAGATTCTT ACCAATGGGT AGTGGGGAAT AGCATAATAG AGAAAGTACA GGTACAAGTA 
ACTGTGCTGC CTGTGGACAA TGTGGGACCC AAGGTCTTCG TCGGGGAGTC CTTCATTGTC 
TATGAAGGTG AGAAGAACTC CTTGACCTTA CAACATCTCC ATGTTGAAGA TGTGGACACT 
CATCAAGATG AACTCCTCTG CACAGTGACT AGTCAGCCAG CCTCTGGCTA CCTGGAAAAG 
ATTGCATCAG CTCCTGGTTC AAAAATGTCC CAGTCTGGTA GCCCTATCAG TGCTTTCTCC 
CTCAGAGATA TCCAAGTGAG GCATATCAAT TATGTACAGA GTATTCACAA GGGAGTAGAG 
CCACAAGAGG ACCAATTCAC CTTTTATTGC TCTGATGGCA TCAACTTCTC CCCAAATGTC 
TTCTTCCCTA TAATCATCCT ACCCACCAAT GATGAGCAGC CTAAACTTTT TGCCCATGAG 
TTTAAGGTAC TAGAGGGGAT GAGCCTGGTC ATAGACACCC AGCTGCTTAA TGGAGCAGAT 
GCTGACCTGC CACCAAATGA GCTCCACTTT CAACTCACAG CCCTCCCTCG GCATGGACGA 
ATCATACAGC AGCTGGCTAC AGGCAGCCAG CCCATCCACA GCTTCACCCT CAAGGAGATC 
CAGGAGGCCT CCACCATTGT GTATGAGCAT GATGACTCAG AGACAAAAGA GGACAGTTTT 
GAGGTCTGGC TGAGTGACGG CAAGCACACA ACCCACAGGA AGGTACCCAT TGTAGTGACC 
CTAGTGGATG ATGAAACTCC TCACCTGACT GTCAACAATG GGCTGAAGGT AGAGAAAGGA 
CACTCTGAGA TCATCACAAA TCGGATCCTC AAGGCCACAG ATCTTGACTC AGATGATAAA 
AGCCTCAGTT TTGTCCTCCA TTCTGGGCCT CAACAAGGGC TTCTACAGAG GCTGAGAAAA 
CCCAGAGGAG AAGTGAGGAA CAATCTTACT CTGGGAATGA ACTTTACCCA GGATGAGATT 
AACAGAGGCC TCATCTGCTA TATCCACACA GGCCAAGAAG GGATTGTTGA CATTATCAAG 
TTTGATGTTA CTGATGGGGT TAACACTTTG ACAGACCACT ACTTCTATGT CACCATTGGC 
AACTTAGACA GCGTCTTCCC TGAGGTAATC AGCAAAAGGA TCACCTTGAT AGAAGGTGCC 
AGAGTGACCC TTACCAACAA TCTGCTTACC AACAGTGACA TCAACAGCTC TGATGAACAT 
CACTTTAGCA TTACACGGGC TCCAAGCCTG GGTCACTTAG AAAGTTCTGA CTATGCTGGG 
GAACCCATTG CCTCTTTCAC TCAACTTCAA TTGGCTAGCA ACAAAATATC CTATGTCCAT 
ACTTCAAATG ATGAGAAAAA GATGGACAGC TTCGAATTTC AAGTGATCGG CGAACTCTAC 
CCTGTGTTCA GAACCTTCAG GATCTTCATC ACTGATGTGG ATAATAAGAA ACCTATCTTG 
ACCATCCATA GACTAACACT ACAGAAAGAG GATAGCCAAC TGATCACTCT CCTTGAGTTG 
ACAGTGGAAG ACAGTGATAC CCCAGATGAC CTTATTCTCT TTACCATCAC CCAGGTCCCC 
ATGCATGGCA AGATTTTGTA CAATGGTAGC CGTCCCGTGA CCACTTTCAC CAAGCAAGAC 
CTGAACAAGA ACCTGATTAG CTACAAGCAT GACGGCAGTG AGACCACTGA AGATAGTTTC 
TCCTTGACTG TGACAGACGG CACTCACACT GATTTCTATG TCCTACCAGA CACTGCCCTG 
GCGACACACA AACCTCAAGT AATGAGGGTC CAGATAAGAT CATTAGACAA TAGGCTTCCC 
CAGATTACTA CCAACAGGGG TGCCCCAGCC TTGAAGCGCC TTCACACTGG ACACATGGGC 
TTCCTGATTA CCAGCAAGTC TCTGAAGGCA GAAGATCAGG ACAGTCCCCA CAGGCTTCTG 
AAGTATAAAG TGACCAGAGG ACCAGAGCAT GGCTTCATTA TCAAAACTGG CCTTGGAAAC 
CAGAGCACTC GAGTGTTTAC ACAAGCTGAC ATTGATGAGA TGAAGATATC CTATGTCTTG 
AATGAGGGCA GCAACGCATC AAAGGACATC TTCTATTTCT CTGTTGAAGA CAATGGAGGA 
AATAAACTAA CAAATCAGCC TTTCCATCTA AACTGGGCTT GGATTTGTTT GGAGAAAGAG 
TACTACATTG TGGATGAAGA TTCCACATTC TTAGAAGTGA CCCTTACACG CAGAGGATAC 
CTTGGAGAAA CATCATTTAT CAGCATTGGT ACCAAAGATG AAACTGCAAA AAAAGACAAA 
GATTTCAAAT GGAAGACCAA TAAACAGATC CAGTTCAATC CTGGACAGAC TACAGCCACA 
TGGAGGGTGA GAATTATACC TGACAATGAA TATGAGACTT CAGAGACCTT CCAGATCATT 
CTGTCTGAAC CTCTCATGGC TGTACTGGAG TTTCCAGAAA TGGCAACGGT TGAAATTGTA 
GACCCTGGAG ATGAATCAAC AGTTTATATT CCAGAGGCGG AATACAAAAT AGAAGAGGAC 
ATTGGGGAAC TTTTGATTCC AGTAAGACGA TCTGGAGATG CAAGCCAGGA ACTAATCGTC 
ATTTGCTCCA CACGTCAAGG TTCTGCCACA GGCACGATTT CTTCCACAGT GTTATTCTCT 
GATTACATCT CACGTCCAGA AGACCACACC AGCATCCTCC ACTTTGACAA GAATGAGACA 
CAGAAGACCT GCCAGGTCCT GATCATTGAT GACTCCCTTT ATGAAGAGGA GGAATCCTTC 
AGCGTTTCAC TTAGGCTGCC AGTGGGAGGA CAGCTGGGAG CCAGATTCCC CACCACTAAG 
GTGACTATTC TGGCTGATCG TTATGATGAA CCTGTCCTGC ACTTTGGGGA TGCTGAATAT 
CACGTCAATG AAAGTGCTCG CTACGTGGAG GTTTGTGTTT GGAGAAGAGG CACTGATCTT 
TCCCAACCAT CATCCATCGC CGTGCGCTCC AGAAAGTCAG AGCAGGAGTC AGCAGAAGCT 
GGAACAGATT ATGTTGGTAT CAGCCGAAAC CTGGACTTTG CTCCAGGCGT GCGCATGCAG 
ACATTCCAAG TGACCATCCT TGATGACCTG GGACAGCCTA CCTTGGAAGG CCCAGAGAAG 
TTTGAATTAC TTCTTCAGAT GCCTATGGGT GCAGTGCTTG GAGAACCAAA TAAAACTACC 
ATTTTCATCG AGGACACCAT CACGGACTGT AAGCAGAGTG CTTGTAGTTC CTTTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161707.1
CDS	1..6420
Translation	MAGASRHPTGTPRQLLVALACLLLSRPALQGRASSLGTEPDPALYLPARGALDGTRPDGPSVLIANPGLRVPLGRSLWLDPLRDLVIGVQPGDRCEVTVLDALPRLKGALSPRRFPCTFGPRQVQYTHFGSHSPGRARVLLQLRYDAPTHTLVLPFTLAVDLVFSQLELVTRNRPLVVEKLRSWSRAIDRRVLDFASLKSGATATRRCRLTPLPHEDGPLPKYGRLVDAVGAPLPRGKGVDCEAFLRAGVRYQHTATSSPNRDYVPMMVELLGPEGQDAGSAGVLVREHFQLLVRIRGGAENTPPRPSFMATMMMEVDPLVLTALTPDALAAEDVESDPGDLVFNILNAPTHPPGHPGQQGYVVSTDDPLGLPVSFFTQQELRELKIAYQPPAENSHGERLFQLELEVVDGDGAASDPFAFMVTVKSMNTLVPVASHNRGLVLFEGQSRPLSSTHSIPISDKDNLEEVKMAAVRGLRHGQLVVFGAPAGCKYFTPADLAAGRVVYQHDGSNTYSDNIIFRMEDGHHQVDFLFPLTILPVDDEPPMVNTNTGLSLTEGQVVQISPFVLSATDIDSEDSTIHFVLENQPLKGNEEEPQWELAPGSSHSGHYLGDLLLQQAELPLSTEDEDWHYMEKEGLYEKVVTEWLQRDIMEGRLFYRHLGPHSPQSVMVQLAFHVQDDHDPPNLSKQHIFTIKVQPVDILSPQLYPGTTLEMTVQEYQLTHFQKNFLRYIDQDSDDQNLWYTLLTLPTDTDGNHQVRAGEIVLTDSPDTLIMHFTQAQVNQHKVAYQPPQKLGIAPRVVQFTYQVEDAAGNSVPGTFTLFLQPVDNQPPEVTNRGFAILEGGSFNLSSNELHVTDPDTDIDQIVFILVRGPQHGHLQYFKRCMVPGESFMQADVINGSVSYQHGRDQTTTSDTFHLEVSDGVHHIPITIPISVHPNVANRSPRISLRSSSLLDVSIDVLENKATEITMGVIHGKRKDVGDLMLSFIVKDSPKLGTILVNGLPTERFTQEDLINGRVAYAHTAGEVGFQKQHDAFSLILSKDSYQWVVGNSIIEKVQVQVTVLPVDNVGPKVFVGESFIVYEGEKNSLTLQHLHVEDVDTHQDELLCTVTSQPASGYLEKIASAPGSKMSQSGSPISAFSLRDIQVRHINYVQSIHKGVEPQEDQFTFYCSDGINFSPNVFFPIIILPTNDEQPKLFAHEFKVLEGMSLVIDTQLLNGADADLPPNELHFQLTALPRHGRIIQQLATGSQPIHSFTLKEIQEASTIVYEHDDSETKEDSFEVWLSDGKHTTHRKVPIVVTLVDDETPHLTVNNGLKVEKGHSEIITNRILKATDLDSDDKSLSFVLHSGPQQGLLQRLRKPRGEVRNNLTLGMNFTQDEINRGLICYIHTGQEGIVDIIKFDVTDGVNTLTDHYFYVTIGNLDSVFPEVISKRITLIEGARVTLTNNLLTNSDINSSDEHHFSITRAPSLGHLESSDYAGEPIASFTQLQLASNKISYVHTSNDEKKMDSFEFQVIGELYPVFRTFRIFITDVDNKKPILTIHRLTLQKEDSQLITLLELTVEDSDTPDDLILFTITQVPMHGKILYNGSRPVTTFTKQDLNKNLISYKHDGSETTEDSFSLTVTDGTHTDFYVLPDTALATHKPQVMRVQIRSLDNRLPQITTNRGAPALKRLHTGHMGFLITSKSLKAEDQDSPHRLLKYKVTRGPEHGFIIKTGLGNQSTRVFTQADIDEMKISYVLNEGSNASKDIFYFSVEDNGGNKLTNQPFHLNWAWICLEKEYYIVDEDSTFLEVTLTRRGYLGETSFISIGTKDETAKKDKDFKWKTNKQIQFNPGQTTATWRVRIIPDNEYETSETFQIILSEPLMAVLEFPEMATVEIVDPGDESTVYIPEAEYKIEEDIGELLIPVRRSGDASQELIVICSTRQGSATGTISSTVLFSDYISRPEDHTSILHFDKNETQKTCQVLIIDDSLYEEEESFSVSLRLPVGGQLGARFPTTKVTILADRYDEPVLHFGDAEYHVNESARYVEVCVWRRGTDLSQPSSIAVRSRKSEQESAEAGTDYVGISRNLDFAPGVRMQTFQVTILDDLGQPTLEGPEKFELLLQMPMGAVLGEPNKTTIFIEDTITDCKQSACSSFD

Target ORF information:

RefSeq Version	NM_001168235.2
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 3 (FREM3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168235.2

ORF Insert Sequence:

ATGGCGGGGG CTTCTCGGCA CCCGACTGGG ACGCCCCGGC AGCTCCTTGT GGCGCTCGCC 
TGCCTGCTCT TGAGTCGCCC CGCGCTGCAG GGACGGGCAT CCTCACTTGG GACCGAGCCC 
GACCCGGCGC TTTACCTGCC CGCCCGGGGT GCGCTTGACG GCACTCGCCC CGACGGCCCC 
AGCGTGCTGA TTGCCAACCC TGGACTCCGG GTGCCCCTGG GTCGTTCCCT TTGGCTCGAC 
CCGCTCCGGG ATCTGGTGAT TGGAGTGCAG CCGGGGGACC GGTGCGAAGT CACGGTACTG 
GACGCCCTGC CGCGGCTCAA GGGCGCGCTC TCCCCGCGCC GCTTCCCCTG CACCTTCGGG 
CCCCGCCAAG TCCAGTACAC TCACTTCGGC TCCCACAGCC CCGGACGCGC CCGGGTGCTG 
CTGCAGCTGC GCTACGACGC CCCGACTCAC ACTCTGGTGC TGCCCTTCAC GCTGGCGGTG 
GACTTGGTCT TCTCCCAGCT GGAGCTGGTG ACGCGTAACA GGCCTTTGGT AGTGGAGAAG 
CTGCGAAGCT GGAGCCGCGC CATAGACAGG AGAGTGCTGG ACTTCGCCTC CCTGAAGTCT 
GGAGCCACGG CCACCCGCAG GTGCCGGCTT ACCCCACTTC CTCACGAGGA CGGCCCCCTG 
CCCAAGTACG GGCGCTTGGT GGACGCGGTG GGGGCCCCTC TCCCCAGGGG CAAGGGCGTA 
GACTGTGAGG CTTTCCTCCG TGCTGGGGTG CGCTATCAGC ACACAGCCAC CTCCTCGCCC 
AACCGTGACT ACGTGCCCAT GATGGTGGAG CTGCTGGGGC CTGAGGGCCA AGACGCTGGG 
TCCGCGGGTG TGCTGGTCCG CGAGCACTTC CAGCTGCTCG TGAGGATCCG CGGCGGAGCC 
GAGAACACAC CGCCCAGGCC CAGCTTCATG GCCACGATGA TGATGGAGGT GGATCCACTG 
GTGCTGACAG CCCTGACGCC TGACGCACTG GCCGCGGAGG ACGTCGAGTC AGACCCTGGT 
GACCTGGTGT TCAACATTCT GAACGCCCCC ACTCACCCAC CAGGGCACCC GGGGCAACAG 
GGCTACGTGG TCAGCACCGA CGACCCTCTA GGGCTTCCAG TCTCCTTCTT CACCCAGCAG 
GAGCTGAGGG AGCTGAAGAT TGCCTATCAG CCCCCTGCAG AGAACTCCCA TGGGGAGCGC 
CTCTTTCAGC TGGAGCTGGA GGTGGTGGAC GGAGACGGCG CCGCCTCAGA CCCCTTTGCC 
TTCATGGTGA CAGTGAAATC CATGAATACT CTGGTCCCAG TGGCTAGCCA TAACAGGGGA 
CTTGTGCTTT TTGAAGGTCA GTCAAGGCCC TTGTCCAGCA CCCACAGCAT TCCTATCAGT 
GATAAAGATA ACCTGGAAGA GGTGAAAATG GCTGCAGTCA GGGGCTTGAG ACATGGGCAG 
CTGGTGGTGT TTGGGGCACC TGCTGGGTGC AAGTATTTCA CACCAGCGGA CCTGGCAGCA 
GGGCGAGTGG TGTATCAGCA TGATGGCAGC AACACCTACA GTGACAATAT CATCTTCCGG 
ATGGAGGACG GGCACCACCA AGTGGACTTC CTCTTTCCCC TCACCATCCT ACCTGTGGAT 
GATGAACCAC CAATGGTCAA TACTAACACA GGACTCTCAC TCACTGAAGG GCAGGTGGTC 
CAGATCTCTC CCTTTGTACT GAGTGCTACT GATATTGACT CTGAGGACTC AACCATCCAC 
TTTGTGCTGG AGAACCAGCC CCTAAAAGGA AATGAGGAAG AGCCTCAGTG GGAGTTGGCC 
CCTGGTTCCT CCCACTCAGG CCACTACCTG GGGGACCTGT TGCTGCAGCA GGCTGAACTA 
CCTCTCTCAA CTGAAGATGA AGACTGGCAC TACATGGAAA AGGAAGGGCT TTATGAGAAA 
GTGGTGACTG AGTGGCTACA GAGAGACATA ATGGAAGGGA GACTCTTCTA CCGCCATCTT 
GGACCACACA GCCCTCAATC TGTAATGGTC CAGCTGGCTT TCCATGTGCA GGATGACCAT 
GACCCACCCA ATCTCTCCAA ACAGCACATT TTCACCATCA AGGTCCAACC AGTGGATATA 
CTGAGTCCAC AGCTGTATCC AGGAACTACA CTAGAAATGA CTGTACAAGA ATACCAACTC 
ACTCACTTCC AGAAGAATTT CCTCCGATAC ATTGACCAGG ACTCTGATGA CCAGAACCTA 
TGGTACACCC TACTGACACT CCCGACTGAC ACAGATGGCA ACCACCAAGT CCGGGCTGGA 
GAAATTGTGC TCACTGATTC ACCAGACACA CTCATCATGC ACTTTACCCA AGCCCAGGTA 
AATCAGCATA AAGTTGCCTA CCAGCCTCCA CAGAAATTGG GTATTGCACC ACGGGTTGTG 
CAGTTTACTT ACCAGGTGGA AGATGCTGCA GGCAATAGCG TGCCAGGCAC ATTCACATTA 
TTCCTGCAAC CTGTGGACAA CCAGCCCCCA GAAGTCACCA ACAGAGGCTT TGCTATCTTA 
GAGGGAGGCA GCTTTAACCT CAGCAGTAAT GAGCTGCATG TTACAGACCC AGACACAGAC 
ATTGACCAAA TTGTCTTCAT ATTAGTCCGG GGTCCCCAAC ATGGACACTT GCAGTACTTT 
AAAAGATGTA TGGTCCCAGG GGAATCTTTC ATGCAAGCTG ATGTTATTAA CGGGAGTGTC 
TCTTACCAGC ATGGCAGAGA CCAGACGACT ACCAGTGACA CTTTCCATCT GGAAGTAAGT 
GATGGGGTGC ACCATATACC CATCACCATC CCAATTTCTG TGCATCCCAA TGTGGCTAAC 
AGAAGTCCTA GGATCTCTCT CAGAAGTAGT TCATTATTGG ATGTTTCTAT AGACGTACTA 
GAGAATAAAG CCACTGAAAT TACCATGGGT GTCATCCATG GCAAAAGGAA GGATGTAGGT 
GACTTGATGC TGTCTTTCAT TGTAAAGGAC AGCCCCAAAC TGGGCACTAT TCTGGTAAAT 
GGTTTGCCCA CAGAACGATT CACCCAAGAG GATCTCATCA ATGGGAGAGT AGCCTATGCC 
CACACTGCAG GTGAAGTTGG TTTTCAAAAG CAGCACGATG CCTTCAGCCT CATCCTCTCC 
AAAGATTCTT ACCAATGGGT AGTGGGGAAT AGCATAATAG AGAAAGTACA GGTACAAGTA 
ACTGTGCTGC CTGTGGACAA TGTGGGACCC AAGGTCTTCG TCGGGGAGTC CTTCATTGTC 
TATGAAGGTG AGAAGAACTC CTTGACCTTA CAACATCTCC ATGTTGAAGA TGTGGACACT 
CATCAAGATG AACTCCTCTG CACAGTGACT AGTCAGCCAG CCTCTGGCTA CCTGGAAAAG 
ATTGCATCAG CTCCTGGTTC AAAAATGTCC CAGTCTGGTA GCCCTATCAG TGCTTTCTCC 
CTCAGAGATA TCCAAGTGAG GCATATCAAT TATGTACAGA GTATTCACAA GGGAGTAGAG 
CCACAAGAGG ACCAATTCAC CTTTTATTGC TCTGATGGCA TCAACTTCTC CCCAAATGTC 
TTCTTCCCTA TAATCATCCT ACCCACCAAT GATGAGCAGC CTAAACTTTT TGCCCATGAG 
TTTAAGGTAC TAGAGGGGAT GAGCCTGGTC ATAGACACCC AGCTGCTTAA TGGAGCAGAT 
GCTGACCTGC CACCAAATGA GCTCCACTTT CAACTCACAG CCCTCCCTCG GCATGGACGA 
ATCATACAGC AGCTGGCTAC AGGCAGCCAG CCCATCCACA GCTTCACCCT CAAGGAGATC 
CAGGAGGCCT CCACCATTGT GTATGAGCAT GATGACTCAG AGACAAAAGA GGACAGTTTT 
GAGGTCTGGC TGAGTGACGG CAAGCACACA ACCCACAGGA AGGTACCCAT TGTAGTGACC 
CTAGTGGATG ATGAAACTCC TCACCTGACT GTCAACAATG GGCTGAAGGT AGAGAAAGGA 
CACTCTGAGA TCATCACAAA TCGGATCCTC AAGGCCACAG ATCTTGACTC AGATGATAAA 
AGCCTCAGTT TTGTCCTCCA TTCTGGGCCT CAACAAGGGC TTCTACAGAG GCTGAGAAAA 
CCCAGAGGAG AAGTGAGGAA CAATCTTACT CTGGGAATGA ACTTTACCCA GGATGAGATT 
AACAGAGGCC TCATCTGCTA TATCCACACA GGCCAAGAAG GGATTGTTGA CATTATCAAG 
TTTGATGTTA CTGATGGGGT TAACACTTTG ACAGACCACT ACTTCTATGT CACCATTGGC 
AACTTAGACA GCGTCTTCCC TGAGGTAATC AGCAAAAGGA TCACCTTGAT AGAAGGTGCC 
AGAGTGACCC TTACCAACAA TCTGCTTACC AACAGTGACA TCAACAGCTC TGATGAACAT 
CACTTTAGCA TTACACGGGC TCCAAGCCTG GGTCACTTAG AAAGTTCTGA CTATGCTGGG 
GAACCCATTG CCTCTTTCAC TCAACTTCAA TTGGCTAGCA ACAAAATATC CTATGTCCAT 
ACTTCAAATG ATGAGAAAAA GATGGACAGC TTCGAATTTC AAGTGATCGG CGAACTCTAC 
CCTGTGTTCA GAACCTTCAG GATCTTCATC ACTGATGTGG ATAATAAGAA ACCTATCTTG 
ACCATCCATA GACTAACACT ACAGAAAGAG GATAGCCAAC TGATCACTCT CCTTGAGTTG 
ACAGTGGAAG ACAGTGATAC CCCAGATGAC CTTATTCTCT TTACCATCAC CCAGGTCCCC 
ATGCATGGCA AGATTTTGTA CAATGGTAGC CGTCCCGTGA CCACTTTCAC CAAGCAAGAC 
CTGAACAAGA ACCTGATTAG CTACAAGCAT GACGGCAGTG AGACCACTGA AGATAGTTTC 
TCCTTGACTG TGACAGACGG CACTCACACT GATTTCTATG TCCTACCAGA CACTGCCCTG 
GCGACACACA AACCTCAAGT AATGAGGGTC CAGATAAGAT CATTAGACAA TAGGCTTCCC 
CAGATTACTA CCAACAGGGG TGCCCCAGCC TTGAAGCGCC TTCACACTGG ACACATGGGC 
TTCCTGATTA CCAGCAAGTC TCTGAAGGCA GAAGATCAGG ACAGTCCCCA CAGGCTTCTG 
AAGTATAAAG TGACCAGAGG ACCAGAGCAT GGCTTCATTA TCAAAACTGG CCTTGGAAAC 
CAGAGCACTC GAGTGTTTAC ACAAGCTGAC ATTGATGAGA TGAAGATATC CTATGTCTTG 
AATGAGGGCA GCAACGCATC AAAGGACATC TTCTATTTCT CTGTTGAAGA CAATGGAGGA 
AATAAACTAA CAAATCAGCC TTTCCATCTA AACTGGGCTT GGATTTGTTT GGAGAAAGAG 
TACTACATTG TGGATGAAGA TTCCACATTC TTAGAAGTGA CCCTTACACG CAGAGGATAC 
CTTGGAGAAA CATCATTTAT CAGCATTGGT ACCAAAGATG AAACTGCAAA AAAAGACAAA 
GATTTCAAAT GGAAGACCAA TAAACAGATC CAGTTCAATC CTGGACAGAC TACAGCCACA 
TGGAGGGTGA GAATTATACC TGACAATGAA TATGAGACTT CAGAGACCTT CCAGATCATT 
CTGTCTGAAC CTCTCATGGC TGTACTGGAG TTTCCAGAAA TGGCAACGGT TGAAATTGTA 
GACCCTGGAG ATGAATCAAC AGTTTATATT CCAGAGGCGG AATACAAAAT AGAAGAGGAC 
ATTGGGGAAC TTTTGATTCC AGTAAGACGA TCTGGAGATG CAAGCCAGGA ACTAATCGTC 
ATTTGCTCCA CACGTCAAGG TTCTGCCACA GGCACGATTT CTTCCACAGT GTTATTCTCT 
GATTACATCT CACGTCCAGA AGACCACACC AGCATCCTCC ACTTTGACAA GAATGAGACA 
CAGAAGACCT GCCAGGTCCT GATCATTGAT GACTCCCTTT ATGAAGAGGA GGAATCCTTC 
AGCGTTTCAC TTAGGCTGCC AGTGGGAGGA CAGCTGGGAG CCAGATTCCC CACCACTAAG 
GTGACTATTC TGGCTGATCG TTATGATGAA CCTGTCCTGC ACTTTGGGGA TGCTGAATAT 
CACGTCAATG AAAGTGCTCG CTACGTGGAG GTTTGTGTTT GGAGAAGAGG CACTGATCTT 
TCCCAACCAT CATCCATCGC CGTGCGCTCC AGAAAGTCAG AGCAGGAGTC AGCAGAAGCT 
GGAACAGATT ATGTTGGTAT CAGCCGAAAC CTGGACTTTG CTCCAGGCGT GCGCATGCAG 
ACATTCCAAG TGACCATCCT TGATGACCTG GGACAGCCTA CCTTGGAAGG CCCAGAGAAG 
TTTGAATTAC TTCTTCAGAT GCCTATGGGT GCAGTGCTTG GAGAACCAAA TAAAACTACC 
ATTTTCATCG AGGACACCAT CACGGACTGT AAGCAGAGTG CTTGTAGTTC CTTTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FRAS1-related extracellular matrix 3 (FREM3) single-nucleotide polymorphism effects on gene expression, amygdala reactivity and perceptual processing speed: An accelerated aging pathway of depression risk.
Frontiers in psychology61377(2015)
Nikolova YS,Iruku SP,Lin CW,Conley ED,Puralewski R,French B,Hariri AR,Sibille E

Comparison of tear protein levels in breast cancer patients and healthy controls using a de novo proteomic approach.
Oncology reports28(2)429-38(2012 Aug)
B?hm D,Keller K,Pieter J,Boehm N,Wolters D,Siggelkow W,Lebrecht A,Schmidt M,K?lbl H,Pfeiffer N,Grus FH

A study of the combined effects of the EHD3 and FREM3 genes in patients with major depressive disorder.
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics159B(3)336-42(2012 Apr)
Shi C,Zhang K,Wang X,Shen Y,Xu Q

Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury.
Circulation125(6)789-802(2012 Feb)
Barallobre-Barreiro J,Didangelos A,Schoendube FA,Drozdov I,Yin X,Fern?ndez-Caggiano M,Willeit P,Puntmann VO,Aldama-L?pez G,Shah AM,Dom?nech N,Mayr M

The Fras1/Frem family of extracellular matrix proteins: structure, function, and association with Fraser syndrome and the mouse bleb phenotype.
Connective tissue research49(3)277-82(2008)
Petrou P,Makrygiannis AK,Chalepakis G