CYLC1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CYLC1
Entrez Gene ID	1538
Full Name	cylicin 1
Synonyms	CYCL1
General protein information	Preferred Names cylicin 1 Names cylicin-1 cylicin I cylicin, basic protein of sperm head cytoskeleton 1 multiple-band polypeptide I
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq21.1
Summary	This gene encodes a sperm head cytoskeletal protein. The encoded protein is associated with the calyx of spermatozoa and spermatids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012].
Disorder MIM:	300768

mRNA and Protein(s)

mRNA	Protein	Name
XM_005262086.4	XP_005262143.1	cylicin-1 isoform X1
XM_005262086.3	XP_005262143.1	cylicin-1 isoform X1
NM_021118.3	NP_066941.1	cylicin-1 isoform 1
NM_001271680.1	NP_001258609.1	cylicin-1 isoform 2
NM_021118.2	NP_066941.1	cylicin-1 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CYLC1	NP_066941.1
Pan troglodytes (chimpanzee)	CYLC1	XP_003954076.1
	LOC713478	XP_001102833.2
Canis lupus familiaris (dog)	CYLC1	XP_005642160.1
Bos taurus (cattle)	CYLC1	NP_776727.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

X chromosomal variation is associated with slow progression to AIDS in HIV-1-infected women.
Siddiqui RA, Sauermann U, Altm?ller J, Fritzer E, Nothnagel M, Dalibor N, Fellay J, Kaup FJ, Stahl-Hennig C, N?rnberg P, Krawczak M, Platzer M
American journal of human genetics85(2)228-39(2009 Aug)

Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation.
Ficarro S, Chertihin O, Westbrook VA, White F, Jayes F, Kalab P, Marto JA, Shabanowitz J, Herr JC, Hunt DF, Visconti PE
The Journal of biological chemistry278(13)11579-89(2003 Mar)

The protein complexity of the cytoskeleton of bovine and human sperm heads: the identification and characterization of cylicin II.
Hess H, Heid H, Zimbelmann R, Franke WW
Experimental cell research218(1)174-82(1995 May)

Molecular characterization of mammalian cylicin, a basic protein of the sperm head cytoskeleton.
Hess H, Heid H, Franke WW
The Journal of cell biology122(5)1043-52(1993 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: X chromosomal variation is associated with slow progression to AIDS in HIV-1-infected women.

The following CYLC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYLC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03599	NM_021118.3 Latest version!	Homo sapiens cylicin 1 (CYLC1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu01193	NM_001271680.1 Latest version!	Homo sapiens cylicin 1 (CYLC1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu54127	XM_005262086.4 Latest version!	Homo sapiens cylicin 1 (CYLC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu54127	XM_005262086.3	Homo sapiens cylicin 1 (CYLC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu03599	NM_021118.2	Homo sapiens cylicin 1 (CYLC1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CYLC1 ( NM_021118.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066941.1 Homo sapiens cylicin 1 (CYLC1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03599
Clone ID Related Accession (Same CDS sequence)	NM_021118.3 , NM_021118.2
Accession Version	NM_021118.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	cylicin-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058019.1, BC126461.1, Z22780.1 and BX110674.1. On May 17, 2019 this sequence version replaced NM_021118.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC126461.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000329312.5/ ENSP00000331556.4 RefSeq Select criteria :: based on longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTTC CAAGGTTGCT AAAAGTAAAC ATCAGAACAT ATGATAATTC CATTCCAATC 
AGTGAATCAA GCAGAAAATC ATGGAATCAA AAACACTTTG CTTTGACATT TCCCAAACCA 
CTCCAGAGAG GTACAAATGA TAAATCAAGA CCTTTGAAAT CACAAATAAC AGTTACTAGA 
CATGACAAAA GAAAACTAGA AGAAGGCCAG AAACCAGCTC ATAAATGGAT AAGGCATTCT 
TTCAGAAAAA TTTTGCAATG GCCACCCATT TACACAGCTG CCAGGGAACA GACTCCATTC 
AGACATCTTT ATACTTCCAA AACCCATCTT AAAAAAGCAG AATATAAAAA GTCCAAAGAT 
GAAAAAGGAG GAACACCTTT GAAGAAAGAT TCCAAGAAAA AAGGAGGTTC ATATGCAACA 
AATCCAGAAT CCAAGCAAAT AGTAGAAGAG AAAACTAAAA GACAAAATGA GGCAGATAAA 
ACTCCCTTAA AATCATCACA TGAAAATGAA CAATCCAAGA AGTCAAAATC CAGTTCAGAA 
ACTAATCCAG AATCCCAAAA TTCTAAGACA GTCTCAAAAA ATTGTTCACA AAAAGATAAG 
AAAGATTCAA AGAATTCCAA GAAGACAAAC ACTGAATTCC TACATACAAA GAACAATCCA 
AAGAAAGATT TGAAGAGGTC AAAGACTAGT AATGATCCCA TATCAGAGAT TTGCTCAGAA 
AATAGTTTAA ATGTTGATTT CCTCATGTTA GTGGGACAGT CTGATGATGA ATCCATAAAT 
TTTGATGCAT GGTTAAGGAA TTACTCACAG AATAATTCAA AGAATTATTC TTTGAAGTAT 
ACAAAGTATA CAAAGAAGGA CACAAAAAAG AATGCAAAGA AAAGCTCTGA TGCTGAATCT 
GAAGACTCAA AGGATGCTAA GAAAGATTCA AAGAAAGTTA AGAAAAATGT CAAGAAAGAT 
GACAAGAAAA AGGATGTAAA GAAGGACACA GAGTCTACTG ATGCTGAATC TGGAGACTCA 
AAGGATGAAA GGAAAGATAC AAAGAAGGAT AAGAAAAAAT TAAAGAAAGA TGACAAGAAA 
AAGGACACAA AGAAGTACCC AGAGTCTACT GATACTGAAT CAGGAGATGC AAAGGATGCA 
AGAAATGATT CAAGAAATTT GAAGAAAGCT TCAAAGAATG ATGACAAGAA AAAGGATGCA 
AAGAAAATTA CATTCTCTAC TGATTCTGAA TCTGAACTGG AGTCAAAGGA GAGTCAGAAA 
GATGAAAAAA AGGATAAAAA AGATTCAAAG ACAGATAATA AAAAGTCTGT CAAGAATGAT 
GAAGAGTCTA CTGATGCTGA CTCTGAACCG AAGGGAGATT CAAAAAAGGG TAAAAAGGAT 
GAAAAGAAGG GGAAGAAAGA TTCAAAGAAA GATGACAAAA AGAAGGATGC AAAGAAAAAT 
GCAGAATCTA CTGAAATGGA ATCTGATTTG GAGTTAAAGA AGGACAAGAA ACACTCAAAG 
GAAAAGAAAG GTTCAAAGAA AGATATCAAG AAGGATGCAA GAAAGGACAC AGAGTCTACT 
GATGCTGAAT TTGATGAATC TTCCAAGACA GGCTTTAAAA CATCTACAAA AATCAAAGGT 
TCAGATACTG AATCTGAAGA GTCACTATAT AAACCTGGGG CTAAGAAGAA AATTGATGAA 
TCAGATGGCA CATCTGCAAA TTCAAAGATG GAAGGACTGG AATCAAAGAG AGGATTCAGA 
ATGTCATCCA AAAAGACTAC ATTCAATGAA AAAGGGGAAA AAGCAAGTAC AGGTAGAGTT 
CCTCCATCAA GAGAAAAACC ACCACTCCCT GCTTGTGAGC CTTCTCTACC ATCACCAAAG 
GTCAGACGTC TTTGTTGGTG CAAGATGCCT CCTCCACCTC CAAAACCAAG ATATGCTCCT 
TTGCCTGAAG CACCGTGGAT TCATAAGCTG CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066941.1
CDS	38..1993
Translation	MSLPRLLKVNIRTYDNSIPISESSRKSWNQKHFALTFPKPLQRGTNDKSRPLKSQITVTRHDKRKLEEGQKPAHKWIRHSFRKILQWPPIYTAAREQTPFRHLYTSKTHLKKAEYKKSKDEKGGTPLKKDSKKKGGSYATNPESKQIVEEKTKRQNEADKTPLKSSHENEQSKKSKSSSETNPESQNSKTVSKNCSQKDKKDSKNSKKTNTEFLHTKNNPKKDLKRSKTSNDPISEICSENSLNVDFLMLVGQSDDESINFDAWLRNYSQNNSKNYSLKYTKYTKKDTKKNAKKSSDAESEDSKDAKKDSKKVKKNVKKDDKKKDVKKDTESTDAESGDSKDERKDTKKDKKKLKKDDKKKDTKKYPESTDTESGDAKDARNDSRNLKKASKNDDKKKDAKKITFSTDSESELESKESQKDEKKDKKDSKTDNKKSVKNDEESTDADSEPKGDSKKGKKDEKKGKKDSKKDDKKKDAKKNAESTEMESDLELKKDKKHSKEKKGSKKDIKKDARKDTESTDAEFDESSKTGFKTSTKIKGSDTESEESLYKPGAKKKIDESDGTSANSKMEGLESKRGFRMSSKKTTFNEKGEKASTGRVPPSREKPPLPACEPSLPSPKVRRLCWCKMPPPPPKPRYAPLPEAPWIHKLL

Target ORF information:

RefSeq Version	NM_021118.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cylicin 1 (CYLC1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021118.3

ORF Insert Sequence:

ATGTCTCTTC CAAGGTTGCT AAAAGTAAAC ATCAGAACAT ATGATAATTC CATTCCAATC 
AGTGAATCAA GCAGAAAATC ATGGAATCAA AAACACTTTG CTTTGACATT TCCCAAACCA 
CTCCAGAGAG GTACAAATGA TAAATCAAGA CCTTTGAAAT CACAAATAAC AGTTACTAGA 
CATGACAAAA GAAAACTAGA AGAAGGCCAG AAACCAGCTC ATAAATGGAT AAGGCATTCT 
TTCAGAAAAA TTTTGCAATG GCCACCCATT TACACAGCTG CCAGGGAACA GACTCCATTC 
AGACATCTTT ATACTTCCAA AACCCATCTT AAAAAAGCAG AATATAAAAA GTCCAAAGAT 
GAAAAAGGAG GAACACCTTT GAAGAAAGAT TCCAAGAAAA AAGGAGGTTC ATATGCAACA 
AATCCAGAAT CCAAGCAAAT AGTAGAAGAG AAAACTAAAA GACAAAATGA GGCAGATAAA 
ACTCCCTTAA AATCATCACA TGAAAATGAA CAATCCAAGA AGTCAAAATC CAGTTCAGAA 
ACTAATCCAG AATCCCAAAA TTCTAAGACA GTCTCAAAAA ATTGTTCACA AAAAGATAAG 
AAAGATTCAA AGAATTCCAA GAAGACAAAC ACTGAATTCC TACATACAAA GAACAATCCA 
AAGAAAGATT TGAAGAGGTC AAAGACTAGT AATGATCCCA TATCAGAGAT TTGCTCAGAA 
AATAGTTTAA ATGTTGATTT CCTCATGTTA GTGGGACAGT CTGATGATGA ATCCATAAAT 
TTTGATGCAT GGTTAAGGAA TTACTCACAG AATAATTCAA AGAATTATTC TTTGAAGTAT 
ACAAAGTATA CAAAGAAGGA CACAAAAAAG AATGCAAAGA AAAGCTCTGA TGCTGAATCT 
GAAGACTCAA AGGATGCTAA GAAAGATTCA AAGAAAGTTA AGAAAAATGT CAAGAAAGAT 
GACAAGAAAA AGGATGTAAA GAAGGACACA GAGTCTACTG ATGCTGAATC TGGAGACTCA 
AAGGATGAAA GGAAAGATAC AAAGAAGGAT AAGAAAAAAT TAAAGAAAGA TGACAAGAAA 
AAGGACACAA AGAAGTACCC AGAGTCTACT GATACTGAAT CAGGAGATGC AAAGGATGCA 
AGAAATGATT CAAGAAATTT GAAGAAAGCT TCAAAGAATG ATGACAAGAA AAAGGATGCA 
AAGAAAATTA CATTCTCTAC TGATTCTGAA TCTGAACTGG AGTCAAAGGA GAGTCAGAAA 
GATGAAAAAA AGGATAAAAA AGATTCAAAG ACAGATAATA AAAAGTCTGT CAAGAATGAT 
GAAGAGTCTA CTGATGCTGA CTCTGAACCG AAGGGAGATT CAAAAAAGGG TAAAAAGGAT 
GAAAAGAAGG GGAAGAAAGA TTCAAAGAAA GATGACAAAA AGAAGGATGC AAAGAAAAAT 
GCAGAATCTA CTGAAATGGA ATCTGATTTG GAGTTAAAGA AGGACAAGAA ACACTCAAAG 
GAAAAGAAAG GTTCAAAGAA AGATATCAAG AAGGATGCAA GAAAGGACAC AGAGTCTACT 
GATGCTGAAT TTGATGAATC TTCCAAGACA GGCTTTAAAA CATCTACAAA AATCAAAGGT 
TCAGATACTG AATCTGAAGA GTCACTATAT AAACCTGGGG CTAAGAAGAA AATTGATGAA 
TCAGATGGCA CATCTGCAAA TTCAAAGATG GAAGGACTGG AATCAAAGAG AGGATTCAGA 
ATGTCATCCA AAAAGACTAC ATTCAATGAA AAAGGGGAAA AAGCAAGTAC AGGTAGAGTT 
CCTCCATCAA GAGAAAAACC ACCACTCCCT GCTTGTGAGC CTTCTCTACC ATCACCAAAG 
GTCAGACGTC TTTGTTGGTG CAAGATGCCT CCTCCACCTC CAAAACCAAG ATATGCTCCT 
TTGCCTGAAG CACCGTGGAT TCATAAGCTG CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYLC1 ( NM_021118.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_066941.1 Homo sapiens cylicin 1 (CYLC1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03599
Clone ID Related Accession (Same CDS sequence)	NM_021118.3 , NM_021118.2
Accession Version	NM_021118.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	cylicin-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB049397.1, BC058019.1, BC126461.1, Z22780.1 and BX110674.1. On Nov 7, 2012 this sequence version replaced NM_021118.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC126461.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTTC CAAGGTTGCT AAAAGTAAAC ATCAGAACAT ATGATAATTC CATTCCAATC 
AGTGAATCAA GCAGAAAATC ATGGAATCAA AAACACTTTG CTTTGACATT TCCCAAACCA 
CTCCAGAGAG GTACAAATGA TAAATCAAGA CCTTTGAAAT CACAAATAAC AGTTACTAGA 
CATGACAAAA GAAAACTAGA AGAAGGCCAG AAACCAGCTC ATAAATGGAT AAGGCATTCT 
TTCAGAAAAA TTTTGCAATG GCCACCCATT TACACAGCTG CCAGGGAACA GACTCCATTC 
AGACATCTTT ATACTTCCAA AACCCATCTT AAAAAAGCAG AATATAAAAA GTCCAAAGAT 
GAAAAAGGAG GAACACCTTT GAAGAAAGAT TCCAAGAAAA AAGGAGGTTC ATATGCAACA 
AATCCAGAAT CCAAGCAAAT AGTAGAAGAG AAAACTAAAA GACAAAATGA GGCAGATAAA 
ACTCCCTTAA AATCATCACA TGAAAATGAA CAATCCAAGA AGTCAAAATC CAGTTCAGAA 
ACTAATCCAG AATCCCAAAA TTCTAAGACA GTCTCAAAAA ATTGTTCACA AAAAGATAAG 
AAAGATTCAA AGAATTCCAA GAAGACAAAC ACTGAATTCC TACATACAAA GAACAATCCA 
AAGAAAGATT TGAAGAGGTC AAAGACTAGT AATGATCCCA TATCAGAGAT TTGCTCAGAA 
AATAGTTTAA ATGTTGATTT CCTCATGTTA GTGGGACAGT CTGATGATGA ATCCATAAAT 
TTTGATGCAT GGTTAAGGAA TTACTCACAG AATAATTCAA AGAATTATTC TTTGAAGTAT 
ACAAAGTATA CAAAGAAGGA CACAAAAAAG AATGCAAAGA AAAGCTCTGA TGCTGAATCT 
GAAGACTCAA AGGATGCTAA GAAAGATTCA AAGAAAGTTA AGAAAAATGT CAAGAAAGAT 
GACAAGAAAA AGGATGTAAA GAAGGACACA GAGTCTACTG ATGCTGAATC TGGAGACTCA 
AAGGATGAAA GGAAAGATAC AAAGAAGGAT AAGAAAAAAT TAAAGAAAGA TGACAAGAAA 
AAGGACACAA AGAAGTACCC AGAGTCTACT GATACTGAAT CAGGAGATGC AAAGGATGCA 
AGAAATGATT CAAGAAATTT GAAGAAAGCT TCAAAGAATG ATGACAAGAA AAAGGATGCA 
AAGAAAATTA CATTCTCTAC TGATTCTGAA TCTGAACTGG AGTCAAAGGA GAGTCAGAAA 
GATGAAAAAA AGGATAAAAA AGATTCAAAG ACAGATAATA AAAAGTCTGT CAAGAATGAT 
GAAGAGTCTA CTGATGCTGA CTCTGAACCG AAGGGAGATT CAAAAAAGGG TAAAAAGGAT 
GAAAAGAAGG GGAAGAAAGA TTCAAAGAAA GATGACAAAA AGAAGGATGC AAAGAAAAAT 
GCAGAATCTA CTGAAATGGA ATCTGATTTG GAGTTAAAGA AGGACAAGAA ACACTCAAAG 
GAAAAGAAAG GTTCAAAGAA AGATATCAAG AAGGATGCAA GAAAGGACAC AGAGTCTACT 
GATGCTGAAT TTGATGAATC TTCCAAGACA GGCTTTAAAA CATCTACAAA AATCAAAGGT 
TCAGATACTG AATCTGAAGA GTCACTATAT AAACCTGGGG CTAAGAAGAA AATTGATGAA 
TCAGATGGCA CATCTGCAAA TTCAAAGATG GAAGGACTGG AATCAAAGAG AGGATTCAGA 
ATGTCATCCA AAAAGACTAC ATTCAATGAA AAAGGGGAAA AAGCAAGTAC AGGTAGAGTT 
CCTCCATCAA GAGAAAAACC ACCACTCCCT GCTTGTGAGC CTTCTCTACC ATCACCAAAG 
GTCAGACGTC TTTGTTGGTG CAAGATGCCT CCTCCACCTC CAAAACCAAG ATATGCTCCT 
TTGCCTGAAG CACCGTGGAT TCATAAGCTG CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066941.1
CDS	58..2013
Translation	MSLPRLLKVNIRTYDNSIPISESSRKSWNQKHFALTFPKPLQRGTNDKSRPLKSQITVTRHDKRKLEEGQKPAHKWIRHSFRKILQWPPIYTAAREQTPFRHLYTSKTHLKKAEYKKSKDEKGGTPLKKDSKKKGGSYATNPESKQIVEEKTKRQNEADKTPLKSSHENEQSKKSKSSSETNPESQNSKTVSKNCSQKDKKDSKNSKKTNTEFLHTKNNPKKDLKRSKTSNDPISEICSENSLNVDFLMLVGQSDDESINFDAWLRNYSQNNSKNYSLKYTKYTKKDTKKNAKKSSDAESEDSKDAKKDSKKVKKNVKKDDKKKDVKKDTESTDAESGDSKDERKDTKKDKKKLKKDDKKKDTKKYPESTDTESGDAKDARNDSRNLKKASKNDDKKKDAKKITFSTDSESELESKESQKDEKKDKKDSKTDNKKSVKNDEESTDADSEPKGDSKKGKKDEKKGKKDSKKDDKKKDAKKNAESTEMESDLELKKDKKHSKEKKGSKKDIKKDARKDTESTDAEFDESSKTGFKTSTKIKGSDTESEESLYKPGAKKKIDESDGTSANSKMEGLESKRGFRMSSKKTTFNEKGEKASTGRVPPSREKPPLPACEPSLPSPKVRRLCWCKMPPPPPKPRYAPLPEAPWIHKLL

Target ORF information:

RefSeq Version	NM_021118.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cylicin 1 (CYLC1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021118.2

ORF Insert Sequence:

ATGTCTCTTC CAAGGTTGCT AAAAGTAAAC ATCAGAACAT ATGATAATTC CATTCCAATC 
AGTGAATCAA GCAGAAAATC ATGGAATCAA AAACACTTTG CTTTGACATT TCCCAAACCA 
CTCCAGAGAG GTACAAATGA TAAATCAAGA CCTTTGAAAT CACAAATAAC AGTTACTAGA 
CATGACAAAA GAAAACTAGA AGAAGGCCAG AAACCAGCTC ATAAATGGAT AAGGCATTCT 
TTCAGAAAAA TTTTGCAATG GCCACCCATT TACACAGCTG CCAGGGAACA GACTCCATTC 
AGACATCTTT ATACTTCCAA AACCCATCTT AAAAAAGCAG AATATAAAAA GTCCAAAGAT 
GAAAAAGGAG GAACACCTTT GAAGAAAGAT TCCAAGAAAA AAGGAGGTTC ATATGCAACA 
AATCCAGAAT CCAAGCAAAT AGTAGAAGAG AAAACTAAAA GACAAAATGA GGCAGATAAA 
ACTCCCTTAA AATCATCACA TGAAAATGAA CAATCCAAGA AGTCAAAATC CAGTTCAGAA 
ACTAATCCAG AATCCCAAAA TTCTAAGACA GTCTCAAAAA ATTGTTCACA AAAAGATAAG 
AAAGATTCAA AGAATTCCAA GAAGACAAAC ACTGAATTCC TACATACAAA GAACAATCCA 
AAGAAAGATT TGAAGAGGTC AAAGACTAGT AATGATCCCA TATCAGAGAT TTGCTCAGAA 
AATAGTTTAA ATGTTGATTT CCTCATGTTA GTGGGACAGT CTGATGATGA ATCCATAAAT 
TTTGATGCAT GGTTAAGGAA TTACTCACAG AATAATTCAA AGAATTATTC TTTGAAGTAT 
ACAAAGTATA CAAAGAAGGA CACAAAAAAG AATGCAAAGA AAAGCTCTGA TGCTGAATCT 
GAAGACTCAA AGGATGCTAA GAAAGATTCA AAGAAAGTTA AGAAAAATGT CAAGAAAGAT 
GACAAGAAAA AGGATGTAAA GAAGGACACA GAGTCTACTG ATGCTGAATC TGGAGACTCA 
AAGGATGAAA GGAAAGATAC AAAGAAGGAT AAGAAAAAAT TAAAGAAAGA TGACAAGAAA 
AAGGACACAA AGAAGTACCC AGAGTCTACT GATACTGAAT CAGGAGATGC AAAGGATGCA 
AGAAATGATT CAAGAAATTT GAAGAAAGCT TCAAAGAATG ATGACAAGAA AAAGGATGCA 
AAGAAAATTA CATTCTCTAC TGATTCTGAA TCTGAACTGG AGTCAAAGGA GAGTCAGAAA 
GATGAAAAAA AGGATAAAAA AGATTCAAAG ACAGATAATA AAAAGTCTGT CAAGAATGAT 
GAAGAGTCTA CTGATGCTGA CTCTGAACCG AAGGGAGATT CAAAAAAGGG TAAAAAGGAT 
GAAAAGAAGG GGAAGAAAGA TTCAAAGAAA GATGACAAAA AGAAGGATGC AAAGAAAAAT 
GCAGAATCTA CTGAAATGGA ATCTGATTTG GAGTTAAAGA AGGACAAGAA ACACTCAAAG 
GAAAAGAAAG GTTCAAAGAA AGATATCAAG AAGGATGCAA GAAAGGACAC AGAGTCTACT 
GATGCTGAAT TTGATGAATC TTCCAAGACA GGCTTTAAAA CATCTACAAA AATCAAAGGT 
TCAGATACTG AATCTGAAGA GTCACTATAT AAACCTGGGG CTAAGAAGAA AATTGATGAA 
TCAGATGGCA CATCTGCAAA TTCAAAGATG GAAGGACTGG AATCAAAGAG AGGATTCAGA 
ATGTCATCCA AAAAGACTAC ATTCAATGAA AAAGGGGAAA AAGCAAGTAC AGGTAGAGTT 
CCTCCATCAA GAGAAAAACC ACCACTCCCT GCTTGTGAGC CTTCTCTACC ATCACCAAAG 
GTCAGACGTC TTTGTTGGTG CAAGATGCCT CCTCCACCTC CAAAACCAAG ATATGCTCCT 
TTGCCTGAAG CACCGTGGAT TCATAAGCTG CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYLC1 ( NM_001271680.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258609.1 Homo sapiens cylicin 1 (CYLC1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01193
Clone ID Related Accession (Same CDS sequence)	NM_001271680.1
Accession Version	NM_001271680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	cylicin-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627233.9, DB028115.1 and BX110674.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site and lacks an alternate in-frame exon compared to variant 1. It encodes a shorter protein (isoform 2) compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BX110674.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTTC CAAGGCTAAA AGTAAACATC AGAACATATG ATAATTCCAT TCCAATCAGT 
GAATCAAGCA GAAAATCATG GAATCAAAAA CACTTTGCTT TGACATTTCC CAAACCACTC 
CAGAGAGGTA CAAATGATAA ATCAAGACCT TTGAAATCAC AAATAACAGT TACTCCTGAA 
GCACCGTGGA TTCATAAGCT GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258609.1
CDS	58..264
Translation	MSLPRLKVNIRTYDNSIPISESSRKSWNQKHFALTFPKPLQRGTNDKSRPLKSQITVTPEAPWIHKLL

Target ORF information:

RefSeq Version	NM_001271680.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cylicin 1 (CYLC1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271680.1

ORF Insert Sequence:

ATGTCTCTTC CAAGGCTAAA AGTAAACATC AGAACATATG ATAATTCCAT TCCAATCAGT 
GAATCAAGCA GAAAATCATG GAATCAAAAA CACTTTGCTT TGACATTTCC CAAACCACTC 
CAGAGAGGTA CAAATGATAA ATCAAGACCT TTGAAATCAC AAATAACAGT TACTCCTGAA 
GCACCGTGGA TTCATAAGCT GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYLC1 ( XM_005262086.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005262143.1 Homo sapiens cylicin 1 (CYLC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu54127
Clone ID Related Accession (Same CDS sequence)	XM_005262086.4 , XM_005262086.3
Accession Version	XM_005262086.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cylicin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005262086.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTTC CAAGGCTAAA AGTAAACATC AGAACATATG ATAATTCCAT TCCAATCAGT 
GAATCAAGCA GAAAATCATG GAATCAAAAA CACTTTGCTT TGACATTTCC CAAACCACTC 
CAGAGAGGTA CAAATGATAA ATCAAGACCT TTGAAATCAC AAATAACAGT TACTAGACAT 
GACAAAAGAA AACTAGAAGA AGGCCAGAAA CCAGCTCATA AATGGATAAG GCATTCTTTC 
AGAAAAATTT TGCAATGGCC ACCCATTTAC ACAGCTGCCA GGGAACAGAC TCCATTCAGA 
CATCTTTATA CTTCCAAAAC CCATCTTAAA AAAGCAGAAT ATAAAAAGTC CAAAGATGAA 
AAAGGAGGAA CACCTTTGAA GAAAGATTCC AAGAAAAAAG GAGGTTCATA TGCAACAAAT 
CCAGAATCCA AGCAAATAGT AGAAGAGAAA ACTAAAAGAC AAAATGAGGC AGATAAAACT 
CCCTTAAAAT CATCACATGA AAATGAACAA TCCAAGAAGT CAAAATCCAG TTCAGAAACT 
AATCCAGAAT CCCAAAATTC TAAGACAGTC TCAAAAAATT GTTCACAAAA AGATAAGAAA 
GATTCAAAGA ATTCCAAGAA GACAAACACT GAATTCCTAC ATACAAAGAA CAATCCAAAG 
AAAGATTTGA AGAGGTCAAA GACTAGTAAT GATCCCATAT CAGAGATTTG CTCAGAAAAT 
AGTTTAAATG TTGATTTCCT CATGTTAGTG GGACAGTCTG ATGATGAATC CATAAATTTT 
GATGCATGGT TAAGGAATTA CTCACAGAAT AATTCAAAGA ATTATTCTTT GAAGTATACA 
AAGTATACAA AGAAGGACAC AAAAAAGAAT GCAAAGAAAA GCTCTGATGC TGAATCTGAA 
GACTCAAAGG ATGCTAAGAA AGATTCAAAG AAAGTTAAGA AAAATGTCAA GAAAGATGAC 
AAGAAAAAGG ATGTAAAGAA GGACACAGAG TCTACTGATG CTGAATCTGG AGACTCAAAG 
GATGAAAGGA AAGATACAAA GAAGGATAAG AAAAAATTAA AGAAAGATGA CAAGAAAAAG 
GACACAAAGA AGTACCCAGA GTCTACTGAT ACTGAATCAG GAGATGCAAA GGATGCAAGA 
AATGATTCAA GAAATTTGAA GAAAGCTTCA AAGAATGATG ACAAGAAAAA GGATGCAAAG 
AAAATTACAT TCTCTACTGA TTCTGAATCT GAACTGGAGT CAAAGGAGAG TCAGAAAGAT 
GAAAAAAAGG ATAAAAAAGA TTCAAAGACA GATAATAAAA AGTCTGTCAA GAATGATGAA 
GAGTCTACTG ATGCTGACTC TGAACCGAAG GGAGATTCAA AAAAGGGTAA AAAGGATGAA 
AAGAAGGGGA AGAAAGATTC AAAGAAAGAT GACAAAAAGA AGGATGCAAA GAAAAATGCA 
GAATCTACTG AAATGGAATC TGATTTGGAG TTAAAGAAGG ACAAGAAACA CTCAAAGGAA 
AAGAAAGGTT CAAAGAAAGA TATCAAGAAG GATGCAAGAA AGGACACAGA GTCTACTGAT 
GCTGAATTTG ATGAATCTTC CAAGACAGGC TTTAAAACAT CTACAAAAAT CAAAGGTTCA 
GATACTGAAT CTGAAGAGTC ACTATATAAA CCTGGGGCTA AGAAGAAAAT TGATGAATCA 
GATGGCACAT CTGCAAATTC AAAGATGGAA GGACTGGAAT CAAAGAGAGG ATTCAGAATG 
TCATCCAAAA AGACTACATT CAATGAAAAA GGGGAAAAAG CAAGTACAGG TAGAGTTCCT 
CCATCAAGAG AAAAACCACC ACTCCCTGCT TGTGAGCCTT CTCTACCATC ACCAAAGGTC 
AGACGTCTTT GTTGGTGCAA GATGCCTCCT CCACCTCCAA AACCAAGATA TGCTCCTTTG 
CCTGAAGCAC CGTGGATTCA TAAGCTGCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005262143.1
CDS	49..2001
Translation	MSLPRLKVNIRTYDNSIPISESSRKSWNQKHFALTFPKPLQRGTNDKSRPLKSQITVTRHDKRKLEEGQKPAHKWIRHSFRKILQWPPIYTAAREQTPFRHLYTSKTHLKKAEYKKSKDEKGGTPLKKDSKKKGGSYATNPESKQIVEEKTKRQNEADKTPLKSSHENEQSKKSKSSSETNPESQNSKTVSKNCSQKDKKDSKNSKKTNTEFLHTKNNPKKDLKRSKTSNDPISEICSENSLNVDFLMLVGQSDDESINFDAWLRNYSQNNSKNYSLKYTKYTKKDTKKNAKKSSDAESEDSKDAKKDSKKVKKNVKKDDKKKDVKKDTESTDAESGDSKDERKDTKKDKKKLKKDDKKKDTKKYPESTDTESGDAKDARNDSRNLKKASKNDDKKKDAKKITFSTDSESELESKESQKDEKKDKKDSKTDNKKSVKNDEESTDADSEPKGDSKKGKKDEKKGKKDSKKDDKKKDAKKNAESTEMESDLELKKDKKHSKEKKGSKKDIKKDARKDTESTDAEFDESSKTGFKTSTKIKGSDTESEESLYKPGAKKKIDESDGTSANSKMEGLESKRGFRMSSKKTTFNEKGEKASTGRVPPSREKPPLPACEPSLPSPKVRRLCWCKMPPPPPKPRYAPLPEAPWIHKLL

Target ORF information:

RefSeq Version	XM_005262086.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cylicin 1 (CYLC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262086.4

ORF Insert Sequence:

ATGTCTCTTC CAAGGCTAAA AGTAAACATC AGAACATATG ATAATTCCAT TCCAATCAGT 
GAATCAAGCA GAAAATCATG GAATCAAAAA CACTTTGCTT TGACATTTCC CAAACCACTC 
CAGAGAGGTA CAAATGATAA ATCAAGACCT TTGAAATCAC AAATAACAGT TACTAGACAT 
GACAAAAGAA AACTAGAAGA AGGCCAGAAA CCAGCTCATA AATGGATAAG GCATTCTTTC 
AGAAAAATTT TGCAATGGCC ACCCATTTAC ACAGCTGCCA GGGAACAGAC TCCATTCAGA 
CATCTTTATA CTTCCAAAAC CCATCTTAAA AAAGCAGAAT ATAAAAAGTC CAAAGATGAA 
AAAGGAGGAA CACCTTTGAA GAAAGATTCC AAGAAAAAAG GAGGTTCATA TGCAACAAAT 
CCAGAATCCA AGCAAATAGT AGAAGAGAAA ACTAAAAGAC AAAATGAGGC AGATAAAACT 
CCCTTAAAAT CATCACATGA AAATGAACAA TCCAAGAAGT CAAAATCCAG TTCAGAAACT 
AATCCAGAAT CCCAAAATTC TAAGACAGTC TCAAAAAATT GTTCACAAAA AGATAAGAAA 
GATTCAAAGA ATTCCAAGAA GACAAACACT GAATTCCTAC ATACAAAGAA CAATCCAAAG 
AAAGATTTGA AGAGGTCAAA GACTAGTAAT GATCCCATAT CAGAGATTTG CTCAGAAAAT 
AGTTTAAATG TTGATTTCCT CATGTTAGTG GGACAGTCTG ATGATGAATC CATAAATTTT 
GATGCATGGT TAAGGAATTA CTCACAGAAT AATTCAAAGA ATTATTCTTT GAAGTATACA 
AAGTATACAA AGAAGGACAC AAAAAAGAAT GCAAAGAAAA GCTCTGATGC TGAATCTGAA 
GACTCAAAGG ATGCTAAGAA AGATTCAAAG AAAGTTAAGA AAAATGTCAA GAAAGATGAC 
AAGAAAAAGG ATGTAAAGAA GGACACAGAG TCTACTGATG CTGAATCTGG AGACTCAAAG 
GATGAAAGGA AAGATACAAA GAAGGATAAG AAAAAATTAA AGAAAGATGA CAAGAAAAAG 
GACACAAAGA AGTACCCAGA GTCTACTGAT ACTGAATCAG GAGATGCAAA GGATGCAAGA 
AATGATTCAA GAAATTTGAA GAAAGCTTCA AAGAATGATG ACAAGAAAAA GGATGCAAAG 
AAAATTACAT TCTCTACTGA TTCTGAATCT GAACTGGAGT CAAAGGAGAG TCAGAAAGAT 
GAAAAAAAGG ATAAAAAAGA TTCAAAGACA GATAATAAAA AGTCTGTCAA GAATGATGAA 
GAGTCTACTG ATGCTGACTC TGAACCGAAG GGAGATTCAA AAAAGGGTAA AAAGGATGAA 
AAGAAGGGGA AGAAAGATTC AAAGAAAGAT GACAAAAAGA AGGATGCAAA GAAAAATGCA 
GAATCTACTG AAATGGAATC TGATTTGGAG TTAAAGAAGG ACAAGAAACA CTCAAAGGAA 
AAGAAAGGTT CAAAGAAAGA TATCAAGAAG GATGCAAGAA AGGACACAGA GTCTACTGAT 
GCTGAATTTG ATGAATCTTC CAAGACAGGC TTTAAAACAT CTACAAAAAT CAAAGGTTCA 
GATACTGAAT CTGAAGAGTC ACTATATAAA CCTGGGGCTA AGAAGAAAAT TGATGAATCA 
GATGGCACAT CTGCAAATTC AAAGATGGAA GGACTGGAAT CAAAGAGAGG ATTCAGAATG 
TCATCCAAAA AGACTACATT CAATGAAAAA GGGGAAAAAG CAAGTACAGG TAGAGTTCCT 
CCATCAAGAG AAAAACCACC ACTCCCTGCT TGTGAGCCTT CTCTACCATC ACCAAAGGTC 
AGACGTCTTT GTTGGTGCAA GATGCCTCCT CCACCTCCAA AACCAAGATA TGCTCCTTTG 
CCTGAAGCAC CGTGGATTCA TAAGCTGCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYLC1 ( XM_005262086.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005262143.1 Homo sapiens cylicin 1 (CYLC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu54127
Clone ID Related Accession (Same CDS sequence)	XM_005262086.4 , XM_005262086.3
Accession Version	XM_005262086.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cylicin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005262086.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTTC CAAGGCTAAA AGTAAACATC AGAACATATG ATAATTCCAT TCCAATCAGT 
GAATCAAGCA GAAAATCATG GAATCAAAAA CACTTTGCTT TGACATTTCC CAAACCACTC 
CAGAGAGGTA CAAATGATAA ATCAAGACCT TTGAAATCAC AAATAACAGT TACTAGACAT 
GACAAAAGAA AACTAGAAGA AGGCCAGAAA CCAGCTCATA AATGGATAAG GCATTCTTTC 
AGAAAAATTT TGCAATGGCC ACCCATTTAC ACAGCTGCCA GGGAACAGAC TCCATTCAGA 
CATCTTTATA CTTCCAAAAC CCATCTTAAA AAAGCAGAAT ATAAAAAGTC CAAAGATGAA 
AAAGGAGGAA CACCTTTGAA GAAAGATTCC AAGAAAAAAG GAGGTTCATA TGCAACAAAT 
CCAGAATCCA AGCAAATAGT AGAAGAGAAA ACTAAAAGAC AAAATGAGGC AGATAAAACT 
CCCTTAAAAT CATCACATGA AAATGAACAA TCCAAGAAGT CAAAATCCAG TTCAGAAACT 
AATCCAGAAT CCCAAAATTC TAAGACAGTC TCAAAAAATT GTTCACAAAA AGATAAGAAA 
GATTCAAAGA ATTCCAAGAA GACAAACACT GAATTCCTAC ATACAAAGAA CAATCCAAAG 
AAAGATTTGA AGAGGTCAAA GACTAGTAAT GATCCCATAT CAGAGATTTG CTCAGAAAAT 
AGTTTAAATG TTGATTTCCT CATGTTAGTG GGACAGTCTG ATGATGAATC CATAAATTTT 
GATGCATGGT TAAGGAATTA CTCACAGAAT AATTCAAAGA ATTATTCTTT GAAGTATACA 
AAGTATACAA AGAAGGACAC AAAAAAGAAT GCAAAGAAAA GCTCTGATGC TGAATCTGAA 
GACTCAAAGG ATGCTAAGAA AGATTCAAAG AAAGTTAAGA AAAATGTCAA GAAAGATGAC 
AAGAAAAAGG ATGTAAAGAA GGACACAGAG TCTACTGATG CTGAATCTGG AGACTCAAAG 
GATGAAAGGA AAGATACAAA GAAGGATAAG AAAAAATTAA AGAAAGATGA CAAGAAAAAG 
GACACAAAGA AGTACCCAGA GTCTACTGAT ACTGAATCAG GAGATGCAAA GGATGCAAGA 
AATGATTCAA GAAATTTGAA GAAAGCTTCA AAGAATGATG ACAAGAAAAA GGATGCAAAG 
AAAATTACAT TCTCTACTGA TTCTGAATCT GAACTGGAGT CAAAGGAGAG TCAGAAAGAT 
GAAAAAAAGG ATAAAAAAGA TTCAAAGACA GATAATAAAA AGTCTGTCAA GAATGATGAA 
GAGTCTACTG ATGCTGACTC TGAACCGAAG GGAGATTCAA AAAAGGGTAA AAAGGATGAA 
AAGAAGGGGA AGAAAGATTC AAAGAAAGAT GACAAAAAGA AGGATGCAAA GAAAAATGCA 
GAATCTACTG AAATGGAATC TGATTTGGAG TTAAAGAAGG ACAAGAAACA CTCAAAGGAA 
AAGAAAGGTT CAAAGAAAGA TATCAAGAAG GATGCAAGAA AGGACACAGA GTCTACTGAT 
GCTGAATTTG ATGAATCTTC CAAGACAGGC TTTAAAACAT CTACAAAAAT CAAAGGTTCA 
GATACTGAAT CTGAAGAGTC ACTATATAAA CCTGGGGCTA AGAAGAAAAT TGATGAATCA 
GATGGCACAT CTGCAAATTC AAAGATGGAA GGACTGGAAT CAAAGAGAGG ATTCAGAATG 
TCATCCAAAA AGACTACATT CAATGAAAAA GGGGAAAAAG CAAGTACAGG TAGAGTTCCT 
CCATCAAGAG AAAAACCACC ACTCCCTGCT TGTGAGCCTT CTCTACCATC ACCAAAGGTC 
AGACGTCTTT GTTGGTGCAA GATGCCTCCT CCACCTCCAA AACCAAGATA TGCTCCTTTG 
CCTGAAGCAC CGTGGATTCA TAAGCTGCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005262143.1
CDS	58..2010
Translation	MSLPRLKVNIRTYDNSIPISESSRKSWNQKHFALTFPKPLQRGTNDKSRPLKSQITVTRHDKRKLEEGQKPAHKWIRHSFRKILQWPPIYTAAREQTPFRHLYTSKTHLKKAEYKKSKDEKGGTPLKKDSKKKGGSYATNPESKQIVEEKTKRQNEADKTPLKSSHENEQSKKSKSSSETNPESQNSKTVSKNCSQKDKKDSKNSKKTNTEFLHTKNNPKKDLKRSKTSNDPISEICSENSLNVDFLMLVGQSDDESINFDAWLRNYSQNNSKNYSLKYTKYTKKDTKKNAKKSSDAESEDSKDAKKDSKKVKKNVKKDDKKKDVKKDTESTDAESGDSKDERKDTKKDKKKLKKDDKKKDTKKYPESTDTESGDAKDARNDSRNLKKASKNDDKKKDAKKITFSTDSESELESKESQKDEKKDKKDSKTDNKKSVKNDEESTDADSEPKGDSKKGKKDEKKGKKDSKKDDKKKDAKKNAESTEMESDLELKKDKKHSKEKKGSKKDIKKDARKDTESTDAEFDESSKTGFKTSTKIKGSDTESEESLYKPGAKKKIDESDGTSANSKMEGLESKRGFRMSSKKTTFNEKGEKASTGRVPPSREKPPLPACEPSLPSPKVRRLCWCKMPPPPPKPRYAPLPEAPWIHKLL

Target ORF information:

RefSeq Version	XM_005262086.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cylicin 1 (CYLC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262086.3

ORF Insert Sequence:

ATGTCTCTTC CAAGGCTAAA AGTAAACATC AGAACATATG ATAATTCCAT TCCAATCAGT 
GAATCAAGCA GAAAATCATG GAATCAAAAA CACTTTGCTT TGACATTTCC CAAACCACTC 
CAGAGAGGTA CAAATGATAA ATCAAGACCT TTGAAATCAC AAATAACAGT TACTAGACAT 
GACAAAAGAA AACTAGAAGA AGGCCAGAAA CCAGCTCATA AATGGATAAG GCATTCTTTC 
AGAAAAATTT TGCAATGGCC ACCCATTTAC ACAGCTGCCA GGGAACAGAC TCCATTCAGA 
CATCTTTATA CTTCCAAAAC CCATCTTAAA AAAGCAGAAT ATAAAAAGTC CAAAGATGAA 
AAAGGAGGAA CACCTTTGAA GAAAGATTCC AAGAAAAAAG GAGGTTCATA TGCAACAAAT 
CCAGAATCCA AGCAAATAGT AGAAGAGAAA ACTAAAAGAC AAAATGAGGC AGATAAAACT 
CCCTTAAAAT CATCACATGA AAATGAACAA TCCAAGAAGT CAAAATCCAG TTCAGAAACT 
AATCCAGAAT CCCAAAATTC TAAGACAGTC TCAAAAAATT GTTCACAAAA AGATAAGAAA 
GATTCAAAGA ATTCCAAGAA GACAAACACT GAATTCCTAC ATACAAAGAA CAATCCAAAG 
AAAGATTTGA AGAGGTCAAA GACTAGTAAT GATCCCATAT CAGAGATTTG CTCAGAAAAT 
AGTTTAAATG TTGATTTCCT CATGTTAGTG GGACAGTCTG ATGATGAATC CATAAATTTT 
GATGCATGGT TAAGGAATTA CTCACAGAAT AATTCAAAGA ATTATTCTTT GAAGTATACA 
AAGTATACAA AGAAGGACAC AAAAAAGAAT GCAAAGAAAA GCTCTGATGC TGAATCTGAA 
GACTCAAAGG ATGCTAAGAA AGATTCAAAG AAAGTTAAGA AAAATGTCAA GAAAGATGAC 
AAGAAAAAGG ATGTAAAGAA GGACACAGAG TCTACTGATG CTGAATCTGG AGACTCAAAG 
GATGAAAGGA AAGATACAAA GAAGGATAAG AAAAAATTAA AGAAAGATGA CAAGAAAAAG 
GACACAAAGA AGTACCCAGA GTCTACTGAT ACTGAATCAG GAGATGCAAA GGATGCAAGA 
AATGATTCAA GAAATTTGAA GAAAGCTTCA AAGAATGATG ACAAGAAAAA GGATGCAAAG 
AAAATTACAT TCTCTACTGA TTCTGAATCT GAACTGGAGT CAAAGGAGAG TCAGAAAGAT 
GAAAAAAAGG ATAAAAAAGA TTCAAAGACA GATAATAAAA AGTCTGTCAA GAATGATGAA 
GAGTCTACTG ATGCTGACTC TGAACCGAAG GGAGATTCAA AAAAGGGTAA AAAGGATGAA 
AAGAAGGGGA AGAAAGATTC AAAGAAAGAT GACAAAAAGA AGGATGCAAA GAAAAATGCA 
GAATCTACTG AAATGGAATC TGATTTGGAG TTAAAGAAGG ACAAGAAACA CTCAAAGGAA 
AAGAAAGGTT CAAAGAAAGA TATCAAGAAG GATGCAAGAA AGGACACAGA GTCTACTGAT 
GCTGAATTTG ATGAATCTTC CAAGACAGGC TTTAAAACAT CTACAAAAAT CAAAGGTTCA 
GATACTGAAT CTGAAGAGTC ACTATATAAA CCTGGGGCTA AGAAGAAAAT TGATGAATCA 
GATGGCACAT CTGCAAATTC AAAGATGGAA GGACTGGAAT CAAAGAGAGG ATTCAGAATG 
TCATCCAAAA AGACTACATT CAATGAAAAA GGGGAAAAAG CAAGTACAGG TAGAGTTCCT 
CCATCAAGAG AAAAACCACC ACTCCCTGCT TGTGAGCCTT CTCTACCATC ACCAAAGGTC 
AGACGTCTTT GTTGGTGCAA GATGCCTCCT CCACCTCCAA AACCAAGATA TGCTCCTTTG 
CCTGAAGCAC CGTGGATTCA TAAGCTGCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

X chromosomal variation is associated with slow progression to AIDS in HIV-1-infected women.
American journal of human genetics85(2)228-39(2009 Aug)
Siddiqui RA,Sauermann U,Altm?ller J,Fritzer E,Nothnagel M,Dalibor N,Fellay J,Kaup FJ,Stahl-Hennig C,N?rnberg P,Krawczak M,Platzer M

Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation.
The Journal of biological chemistry278(13)11579-89(2003 Mar)
Ficarro S,Chertihin O,Westbrook VA,White F,Jayes F,Kalab P,Marto JA,Shabanowitz J,Herr JC,Hunt DF,Visconti PE

The protein complexity of the cytoskeleton of bovine and human sperm heads: the identification and characterization of cylicin II.
Experimental cell research218(1)174-82(1995 May)
Hess H,Heid H,Zimbelmann R,Franke WW

Molecular characterization of mammalian cylicin, a basic protein of the sperm head cytoskeleton.
The Journal of cell biology122(5)1043-52(1993 Sep)
Hess H,Heid H,Franke WW