SIX5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SIX5
Entrez Gene ID	147912
Full Name	SIX homeobox 5
Synonyms	BOR2,DMAHP
General protein information	Preferred Names SIX homeobox 5 Names homeobox protein SIX5 DM locus-associated homeodomain protein dystrophia myotonica-associated homeodomain protein sine oculis homeobox homolog 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.32
Summary	The protein encoded by this gene is a homeodomain-containing transcription factor that appears to function in the regulation of organogenesis. This gene is located downstream of the dystrophia myotonica-protein kinase gene. Mutations in this gene are a cause of branchiootorenal syndrome type 2. [provided by RefSeq, Jul 2009].
Disorder MIM:	600963
Disorder Html:	Branchiootorenal syndrome 2, 610896 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_175875.4	NP_787071.2	homeobox protein SIX5
NM_175875.5	NP_787071.3	homeobox protein SIX5

Pathways from BioSystems

Homologies

Homo sapiens (human)	SIX5	NP_787071.2
Mus musculus (house mouse)	Six5	NP_035513.1
Rattus norvegicus (Norway rat)	Six5	XP_003748828.1
Pan troglodytes (chimpanzee)	SIX5	XP_512758.2
Bos taurus (cattle)	SIX5	NP_001192695.1
	SIX5	XP_001111437.1
Canis lupus familiaris (dog)	SIX5	XP_005616928.1

Gene Ontology
Bibliography

Related articles in PubMed

The expression profile and clinic significance of the SIX family in non-small cell lung cancer.
Liu Q, Li A, Tian Y, Liu Y, Li T, Zhang C, Wu JD, Han X, Wu K
Journal of hematology & oncology9(1)119(2016 11)

Branchio-oto-renal syndrome: comprehensive review based on nationwide surveillance in Japan.
Morisada N, Nozu K, Iijima K
Pediatrics international : official journal of the Japan Pediatric Society56(3)309-14(2014 Jun)

Mutation screening of the EYA1, SIX1, and SIX5 genes in an East Asian cohort with branchio-oto-renal syndrome.
Wang SH, Wu CC, Lu YC, Lin YH, Su YN, Hwu WL, Yu IS, Hsu CJ
The Laryngoscope122(5)1130-6(2012 May)

Mutation screening of the EYA1, SIX1, and SIX5 genes in a large cohort of patients harboring branchio-oto-renal syndrome calls into question the pathogenic role of SIX5 mutations.
Krug P, Morini?re V, Marlin S, Koubi V, Gabriel HD, Colin E, Bonneau D, Salomon R, Antignac C, Heidet L
Human mutation32(2)183-90(2011 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the higher expressions of SIX1-5 were associated with the greater possibility of the tumorigenesis in lung neoplasms
Title: The expression profile and clinic significance of the SIX family in non-small cell lung cancer.

Three causative genes for BOR syndrome have been reported thus far: EYA1, SIX1, and SIX5, but the causative genes for approximately half of all BOR patients remain unknown.[review]
Title: Branchio-oto-renal syndrome: comprehensive review based on nationwide surveillance in Japan.

In East Asian populations, a SIX1 mutation has been reported in a Japanese family with branchio-oto (BO) syndrome,yet SIX5 mutations have never been reported.
Title: Mutation screening of the EYA1, SIX1, and SIX5 genes in an East Asian cohort with branchio-oto-renal syndrome.

Study reports a screening of 140 patients from 124 families with Branchio-oto-renal and identified 36 EYA1 mutations in 42 unrelated patients, 2 mutations, and 1 change of unknown significance in SIX1 in 3 unrelated patients, but no mutation in SIX5.
Title: Mutation screening of the EYA1, SIX1, and SIX5 genes in a large cohort of patients harboring branchio-oto-renal syndrome calls into question the pathogenic role of SIX5 mutations.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association of genetic variants with hemorrhagic stroke in Japanese individuals.

The following SIX5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIX5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu107809	NM_175875.5 Latest version!	Homo sapiens SIX homeobox 5 (SIX5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu22479	NM_175875.4 Latest version!	Homo sapiens SIX homeobox 5 (SIX5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$517.30 $739.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIX5 ( NM_175875.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_787071.3 Homo sapiens SIX homeobox 5 (SIX5), mRNA.

Price & Availability↑

CloneID	OHu107809
Clone ID Related Accession (Same CDS sequence)	NM_175875.5
Accession Version	NM_175875.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	homeobox protein SIX5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC074212.5. On Nov 23, 2018 this sequence version replaced NM_175875.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.484181.1, AK074826.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155770, SAMEA2155984 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000317578.7/ ENSP00000316842.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTACCT TGCCTGCGGA GCCGAGCGCG GGGCCGGCGG CTGGGGGGGA GGCGGTGGCG 
GCGGCGGCGG CGACCGAAGA GGAGGAGGAG GAAGCGCGCC AGCTCTTGCA GACTTTGCAG 
GCGGCCGAGG GTGAGGCGGC GGCGGCGGCC GGGGCCGGGG CGGGCGCAGC GGCTGCGGGA 
GCTGAGGGCC CGGGATCCCC GGGCGTCCCC GGGTCGCCCC CCGAGGCCGC TTCCGAACCG 
CCCACGGGCC TCCGCTTCTC GCCCGAGCAG GTGGCGTGCG TCTGCGAGGC GCTGCTCCAG 
GCGGGCCACG CCGGCCGCTT GAGCCGCTTC CTGGGCGCAC TGCCCCCGGC CGAGCGCCTA 
CGTGGCAGCG ACCCGGTGTT GCGCGCGCGG GCCCTGGTGG CCTTCCAGCG GGGCGAGTAC 
GCCGAGCTCT ACCGGCTACT CGAGAGCCGC CCCTTCCCCG CCGCCCACCA CGCCTTCCTG 
CAGGACCTCT ACCTGCGCGC GCGCTACCAT GAGGCCGAGC GGGCCCGCGG CCGCGCGCTT 
GGCGCAGTGG ACAAGTATCG ACTGCGCAAG AAGTTCCCGC TGCCCAAGAC CATCTGGGAC 
GGCGAGGAGA CAGTCTACTG CTTCAAGGAG CGCTCCCGCG CAGCGCTCAA GGCCTGCTAC 
CGCGGCAACC GCTACCCCAC GCCGGACGAG AAGCGCCGCC TGGCCACACT CACCGGCCTG 
TCGCTCACGC AGGTCAGCAA CTGGTTCAAG AACCGGCGAC AGCGCGACCG GACCGGGGCC 
GGAGGCGGCG CGCCCTGCAA GAGCGAGTCT GATGGGAATC CCACGACTGA GGACGAGTCC 
AGCCGAAGTC CTGAGGACCT GGAGAGAGGG GCGGCCCCAG TGTCCGCCGA GGCCGCTGCC 
CAGGGCTCCA TATTCCTGGC AGGGACCGGC CCTCCCGCGC CTTGCCCGGC TTCCTCCTCC 
ATCCTGGTGA ACGGGAGCTT CCTGGCAGCC AGCGGCTCCC CAGCAGTGCT CCTCAACGGG 
GGCCCCGTCA TCATCAACGG CCTGGCCCTG GGCGAGGCCT CCAGCCTGGG CCCGCTGCTG 
CTCACTGGGG GCGGGGGTGC CCCTCCACCG CAGCCCAGCC CTCAGGGGGC CAGCGAGACC 
AAGACCTCTC TGGTCCTGGA CCCTCAGACA GGGGAGGTGC GGCTGGAGGA GGCTCAGTCG 
GAGGCCCCTG AGACCAAAGG GGCCCAGGTG GCTGCTCCGG GACCAGCCCT TGGAGAGGAG 
GTCCTGGGGC CCCTGGCCCA AGTGGTGCCT GGCCCCCCGA CGGCTGCCAC CTTTCCTCTG 
CCCCCGGGGC CAGTGCCTGC TGTGGCTGCC CCACAAGTGG TACCGCTCTC CCCACCCCCG 
GGGTATCCCA CGGGCCTGAG CCCCACCTCC CCACTATTGA ACCTGCCCCA GGTAGTACCC 
ACCTCACAGG TGGTGACCCT GCCCCAGGCT GTGGGGCCCC TGCAGCTGTT GGCAGCCGGG 
CCAGGCAGCC CTGTGAAGGT GGCAGCTGCA GCAGGCCCTG CCAATGTGCA CCTCATCAAC 
TCCGGGGTGG GCGTGACTGC CCTGCAGCTG CCTTCGGCCA CTGCCCCAGG AAACTTCCTC 
CTGGCCAACC CTGTGTCTGG CAGCCCCATC GTGACGGGTG TGGCCCTGCA GCAGGGCAAG 
ATCATCCTCA CCGCCACCTT CCCCACCAGC ATGCTCGTCT CCCAGGTCCT GCCGCCAGCC 
CCCGGCCTGG CCCTGCCACT GAAGCCAGAG ACGGCCATCT CCGTGCCTGA GGGAGGCCTC 
CCGGTGGCCC CCAGCCCTGC TCTCCCAGAG GCTCACGCCC TAGGCACCCT TTCTGCACAG 
CAGCCACCCC CCGCCGCTGC CACCACCTCC AGCACCAGCC TGCCCTTCTC CCCTGACTCC 
CCTGGCCTCC TGCCCAACTT CCCGGCGCCC CCACCAGAGG GGCTGATGTT GTCACCCGCG 
GCCGTGCCTG TCTGGTCAGC AGGGCTGGAA CTAAGCGCAG GAACAGAGGG GCTGCTGGAA 
GCGGAAAAGG GGCTGGGGAC ACAGGCCCCC CACACCGTGC TGAGGCTGCC AGACCCCGAC 
CCTGAGGGGC TGCTCCTGGG GGCCACCGCA GGGGGTGAGG TTGACGAGGG GTTGGAAGCT 
GAGGCCAAGG TTCTGACCCA GCTCCAGTCG GTGCCTGTGG AGGAGCCCTT GGAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787071.3
CDS	409..2628
Translation	MATLPAEPSAGPAAGGEAVAAAAATEEEEEEARQLLQTLQAAEGEAAAAAGAGAGAAAAGAEGPGSPGVPGSPPEAASEPPTGLRFSPEQVACVCEALLQAGHAGRLSRFLGALPPAERLRGSDPVLRARALVAFQRGEYAELYRLLESRPFPAAHHAFLQDLYLRARYHEAERARGRALGAVDKYRLRKKFPLPKTIWDGEETVYCFKERSRAALKACYRGNRYPTPDEKRRLATLTGLSLTQVSNWFKNRRQRDRTGAGGGAPCKSESDGNPTTEDESSRSPEDLERGAAPVSAEAAAQGSIFLAGTGPPAPCPASSSILVNGSFLAASGSPAVLLNGGPVIINGLALGEASSLGPLLLTGGGGAPPPQPSPQGASETKTSLVLDPQTGEVRLEEAQSEAPETKGAQVAAPGPALGEEVLGPLAQVVPGPPTAATFPLPPGPVPAVAAPQVVPLSPPPGYPTGLSPTSPLLNLPQVVPTSQVVTLPQAVGPLQLLAAGPGSPVKVAAAAGPANVHLINSGVGVTALQLPSATAPGNFLLANPVSGSPIVTGVALQQGKIILTATFPTSMLVSQVLPPAPGLALPLKPETAISVPEGGLPVAPSPALPEAHALGTLSAQQPPPAAATTSSTSLPFSPDSPGLLPNFPAPPPEGLMLSPAAVPVWSAGLELSAGTEGLLEAEKGLGTQAPHTVLRLPDPDPEGLLLGATAGGEVDEGLEAEAKVLTQLQSVPVEEPLEL

Target ORF information:

RefSeq Version	NM_175875.5
Organism	Homo sapiens(human)
Definition	Homo sapiens SIX homeobox 5 (SIX5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175875.5

ORF Insert Sequence:

ATGGCTACCT TGCCTGCGGA GCCGAGCGCG GGGCCGGCGG CTGGGGGGGA GGCGGTGGCG 
GCGGCGGCGG CGACCGAAGA GGAGGAGGAG GAAGCGCGCC AGCTCTTGCA GACTTTGCAG 
GCGGCCGAGG GTGAGGCGGC GGCGGCGGCC GGGGCCGGGG CGGGCGCAGC GGCTGCGGGA 
GCTGAGGGCC CGGGATCCCC GGGCGTCCCC GGGTCGCCCC CCGAGGCCGC TTCCGAACCG 
CCCACGGGCC TCCGCTTCTC GCCCGAGCAG GTGGCGTGCG TCTGCGAGGC GCTGCTCCAG 
GCGGGCCACG CCGGCCGCTT GAGCCGCTTC CTGGGCGCAC TGCCCCCGGC CGAGCGCCTA 
CGTGGCAGCG ACCCGGTGTT GCGCGCGCGG GCCCTGGTGG CCTTCCAGCG GGGCGAGTAC 
GCCGAGCTCT ACCGGCTACT CGAGAGCCGC CCCTTCCCCG CCGCCCACCA CGCCTTCCTG 
CAGGACCTCT ACCTGCGCGC GCGCTACCAT GAGGCCGAGC GGGCCCGCGG CCGCGCGCTT 
GGCGCAGTGG ACAAGTATCG ACTGCGCAAG AAGTTCCCGC TGCCCAAGAC CATCTGGGAC 
GGCGAGGAGA CAGTCTACTG CTTCAAGGAG CGCTCCCGCG CAGCGCTCAA GGCCTGCTAC 
CGCGGCAACC GCTACCCCAC GCCGGACGAG AAGCGCCGCC TGGCCACACT CACCGGCCTG 
TCGCTCACGC AGGTCAGCAA CTGGTTCAAG AACCGGCGAC AGCGCGACCG GACCGGGGCC 
GGAGGCGGCG CGCCCTGCAA GAGCGAGTCT GATGGGAATC CCACGACTGA GGACGAGTCC 
AGCCGAAGTC CTGAGGACCT GGAGAGAGGG GCGGCCCCAG TGTCCGCCGA GGCCGCTGCC 
CAGGGCTCCA TATTCCTGGC AGGGACCGGC CCTCCCGCGC CTTGCCCGGC TTCCTCCTCC 
ATCCTGGTGA ACGGGAGCTT CCTGGCAGCC AGCGGCTCCC CAGCAGTGCT CCTCAACGGG 
GGCCCCGTCA TCATCAACGG CCTGGCCCTG GGCGAGGCCT CCAGCCTGGG CCCGCTGCTG 
CTCACTGGGG GCGGGGGTGC CCCTCCACCG CAGCCCAGCC CTCAGGGGGC CAGCGAGACC 
AAGACCTCTC TGGTCCTGGA CCCTCAGACA GGGGAGGTGC GGCTGGAGGA GGCTCAGTCG 
GAGGCCCCTG AGACCAAAGG GGCCCAGGTG GCTGCTCCGG GACCAGCCCT TGGAGAGGAG 
GTCCTGGGGC CCCTGGCCCA AGTGGTGCCT GGCCCCCCGA CGGCTGCCAC CTTTCCTCTG 
CCCCCGGGGC CAGTGCCTGC TGTGGCTGCC CCACAAGTGG TACCGCTCTC CCCACCCCCG 
GGGTATCCCA CGGGCCTGAG CCCCACCTCC CCACTATTGA ACCTGCCCCA GGTAGTACCC 
ACCTCACAGG TGGTGACCCT GCCCCAGGCT GTGGGGCCCC TGCAGCTGTT GGCAGCCGGG 
CCAGGCAGCC CTGTGAAGGT GGCAGCTGCA GCAGGCCCTG CCAATGTGCA CCTCATCAAC 
TCCGGGGTGG GCGTGACTGC CCTGCAGCTG CCTTCGGCCA CTGCCCCAGG AAACTTCCTC 
CTGGCCAACC CTGTGTCTGG CAGCCCCATC GTGACGGGTG TGGCCCTGCA GCAGGGCAAG 
ATCATCCTCA CCGCCACCTT CCCCACCAGC ATGCTCGTCT CCCAGGTCCT GCCGCCAGCC 
CCCGGCCTGG CCCTGCCACT GAAGCCAGAG ACGGCCATCT CCGTGCCTGA GGGAGGCCTC 
CCGGTGGCCC CCAGCCCTGC TCTCCCAGAG GCTCACGCCC TAGGCACCCT TTCTGCACAG 
CAGCCACCCC CCGCCGCTGC CACCACCTCC AGCACCAGCC TGCCCTTCTC CCCTGACTCC 
CCTGGCCTCC TGCCCAACTT CCCGGCGCCC CCACCAGAGG GGCTGATGTT GTCACCCGCG 
GCCGTGCCTG TCTGGTCAGC AGGGCTGGAA CTAAGCGCAG GAACAGAGGG GCTGCTGGAA 
GCGGAAAAGG GGCTGGGGAC ACAGGCCCCC CACACCGTGC TGAGGCTGCC AGACCCCGAC 
CCTGAGGGGC TGCTCCTGGG GGCCACCGCA GGGGGTGAGG TTGACGAGGG GTTGGAAGCT 
GAGGCCAAGG TTCTGACCCA GCTCCAGTCG GTGCCTGTGG AGGAGCCCTT GGAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIX5 ( NM_175875.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_787071.2 Homo sapiens SIX homeobox 5 (SIX5), mRNA.

Price & Availability↑

CloneID	OHu22479
Clone ID Related Accession (Same CDS sequence)	NM_175875.4
Accession Version	NM_175875.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	homeobox protein SIX5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC074212.5, AK074826.1 and BC033204.1. This sequence is a reference standard in the RefSeqGene project. On Jul 24, 2009 this sequence version replaced NM_175875.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.484181.1, AK074826.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155770, SAMEA2155984 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTACCT TGCCTGCGGA GCCGAGCGCG GGGCCGGCGG CTGGGGGGGA GGCGGTGGCG 
GCGGCGGCGG CGACCGAAGA GGAGGAGGAG GAAGCGCGCC AGCTCTTGCA GACTTTGCAG 
GCGGCCGAGG GTGAGGCGGC GGCGGCGGCC GGGGCCGGGG CGGGCGCAGC GGCTGCGGGA 
GCTGAGGGCC CGGGATCCCC GGGCGTCCCC GGGTCGCCCC CCGAGGCCGC TTCCGAACCG 
CCCACGGGCC TCCGCTTCTC GCCCGAGCAG GTGGCGTGCG TCTGCGAGGC GCTGCTCCAG 
GCGGGCCACG CCGGCCGCTT GAGCCGCTTC CTGGGCGCAC TGCCCCCGGC CGAGCGCCTA 
CGTGGCAGCG ACCCGGTGTT GCGCGCGCGG GCCCTGGTGG CCTTCCAGCG GGGCGAGTAC 
GCCGAGCTCT ACCGGCTACT CGAGAGCCGC CCCTTCCCCG CCGCCCACCA CGCCTTCCTG 
CAGGACCTCT ACCTGCGCGC GCGCTACCAT GAGGCCGAGC GGGCCCGCGG CCGCGCGCTT 
GGCGCAGTGG ACAAGTATCG ACTGCGCAAG AAGTTCCCGC TGCCCAAGAC CATCTGGGAC 
GGCGAGGAGA CAGTCTACTG CTTCAAGGAG CGCTCCCGCG CAGCGCTCAA GGCCTGCTAC 
CGCGGCAACC GCTACCCCAC GCCGGACGAG AAGCGCCGCC TGGCCACACT CACCGGCCTG 
TCGCTCACGC AGGTCAGCAA CTGGTTCAAG AACCGGCGAC AGCGCGACCG GACCGGGGCC 
GGAGGCGGCG CGCCCTGCAA GAGCGAGTCT GATGGGAATC CCACGACTGA GGACGAGTCC 
AGCCGAAGTC CTGAGGACCT GGAGAGAGGG GCGGCCCCAG TGTCCGCCGA GGCCGCTGCC 
CAGGGCTCCA TATTCCTGGC AGGGACCGGC CCTCCCGCGC CTTGCCCGGC TTCCTCCTCC 
ATCCTGGTGA ACGGGAGCTT CCTGGCAGCC AGCGGCTCCC CAGCAGTGCT CCTCAACGGG 
GGCCCCGTCA TCATCAACGG CCTGGCCCTG GGCGAGGCCT CCAGCCTGGG CCCGCTGCTG 
CTCACTGGGG GCGGGGGTGC CCCTCCACCG CAGCCCAGCC CTCAGGGGGC CAGCGAGACC 
AAGACCTCTC TGGTCCTGGA CCCTCAGACA GGGGAGGTGC GGCTGGAGGA GGCTCAGTCG 
GAGGCCCCTG AGACCAAAGG GGCCCAGGTG GCTGCTCCGG GACCAGCCCT TGGAGAGGAG 
GTCCTGGGGC CCCTGGCCCA AGTGGTGCCT GGCCCCCCGA CGGCTGCCAC CTTTCCTCTG 
CCCCCGGGGC CAGTGCCTGC TGTGGCTGCC CCACAAGTGG TACCGCTCTC CCCACCCCCG 
GGGTATCCCA CGGGCCTGAG CCCCACCTCC CCACTATTGA ACCTGCCCCA GGTAGTACCC 
ACCTCACAGG TGGTGACCCT GCCCCAGGCT GTGGGGCCCC TGCAGCTGTT GGCAGCCGGG 
CCAGGCAGCC CTGTGAAGGT GGCAGCTGCA GCAGGCCCTG CCAATGTGCA CCTCATCAAC 
TCCGGGGTGG GCGTGACTGC CCTGCAGCTG CCTTCGGCCA CTGCCCCAGG AAACTTCCTC 
CTGGCCAACC CTGTGTCTGG CAGCCCCATC GTGACGGGTG TGGCCGTGCA GCAGGGCAAG 
ATCATCCTCA CCGCCACCTT CCCCACCAGC ATGCTCGTCT CCCAGGTCCT GCCGCCAGCC 
CCCGGCCTGG CCCTGCCACT GAAGCCAGAG ACGGCCATCT CCGTGCCTGA GGGAGGCCTC 
CCGGTGGCCC CCAGCCCTGC TCTCCCAGAG GCTCACGCCC TAGGCACCCT TTCTGCACAG 
CAGCCACCCC CCGCCGCTGC CACCACCTCC AGCACCAGCC TGCCCTTCTC CCCTGACTCC 
CCTGGCCTCC TGCCCAACTT CCCGGCGCCC CCACCAGAGG GGCTGATGTT GTCACCCGCG 
GCCGTGCCTG TCTGGTCAGC AGGGCTGGAA CTAAGCGCAG GAACAGAGGG GCTGCTGGAA 
GCGGAAAAGG GGCTGGGGAC ACAGGCCCCC CACACCGTGC TGAGGCTGCC AGACCCCGAC 
CCTGAGGGGC TGCTCCTGGG GGCCACCGCA GGGGGTGAGG TTGACGAGGG GTTGGAAGCT 
GAGGCCAAGG TTCTGACCCA GCTCCAGTCG GTGCCTGTGG AGGAGCCCTT GGAACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787071.2
CDS	396..2615
Translation	MATLPAEPSAGPAAGGEAVAAAAATEEEEEEARQLLQTLQAAEGEAAAAAGAGAGAAAAGAEGPGSPGVPGSPPEAASEPPTGLRFSPEQVACVCEALLQAGHAGRLSRFLGALPPAERLRGSDPVLRARALVAFQRGEYAELYRLLESRPFPAAHHAFLQDLYLRARYHEAERARGRALGAVDKYRLRKKFPLPKTIWDGEETVYCFKERSRAALKACYRGNRYPTPDEKRRLATLTGLSLTQVSNWFKNRRQRDRTGAGGGAPCKSESDGNPTTEDESSRSPEDLERGAAPVSAEAAAQGSIFLAGTGPPAPCPASSSILVNGSFLAASGSPAVLLNGGPVIINGLALGEASSLGPLLLTGGGGAPPPQPSPQGASETKTSLVLDPQTGEVRLEEAQSEAPETKGAQVAAPGPALGEEVLGPLAQVVPGPPTAATFPLPPGPVPAVAAPQVVPLSPPPGYPTGLSPTSPLLNLPQVVPTSQVVTLPQAVGPLQLLAAGPGSPVKVAAAAGPANVHLINSGVGVTALQLPSATAPGNFLLANPVSGSPIVTGVAVQQGKIILTATFPTSMLVSQVLPPAPGLALPLKPETAISVPEGGLPVAPSPALPEAHALGTLSAQQPPPAAATTSSTSLPFSPDSPGLLPNFPAPPPEGLMLSPAAVPVWSAGLELSAGTEGLLEAEKGLGTQAPHTVLRLPDPDPEGLLLGATAGGEVDEGLEAEAKVLTQLQSVPVEEPLEL

Target ORF information:

RefSeq Version	NM_175875.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SIX homeobox 5 (SIX5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175875.4

ORF Insert Sequence:

ATGGCTACCT TGCCTGCGGA GCCGAGCGCG GGGCCGGCGG CTGGGGGGGA GGCGGTGGCG 
GCGGCGGCGG CGACCGAAGA GGAGGAGGAG GAAGCGCGCC AGCTCTTGCA GACTTTGCAG 
GCGGCCGAGG GTGAGGCGGC GGCGGCGGCC GGGGCCGGGG CGGGCGCAGC GGCTGCGGGA 
GCTGAGGGCC CGGGATCCCC GGGCGTCCCC GGGTCGCCCC CCGAGGCCGC TTCCGAACCG 
CCCACGGGCC TCCGCTTCTC GCCCGAGCAG GTGGCGTGCG TCTGCGAGGC GCTGCTCCAG 
GCGGGCCACG CCGGCCGCTT GAGCCGCTTC CTGGGCGCAC TGCCCCCGGC CGAGCGCCTA 
CGTGGCAGCG ACCCGGTGTT GCGCGCGCGG GCCCTGGTGG CCTTCCAGCG GGGCGAGTAC 
GCCGAGCTCT ACCGGCTACT CGAGAGCCGC CCCTTCCCCG CCGCCCACCA CGCCTTCCTG 
CAGGACCTCT ACCTGCGCGC GCGCTACCAT GAGGCCGAGC GGGCCCGCGG CCGCGCGCTT 
GGCGCAGTGG ACAAGTATCG ACTGCGCAAG AAGTTCCCGC TGCCCAAGAC CATCTGGGAC 
GGCGAGGAGA CAGTCTACTG CTTCAAGGAG CGCTCCCGCG CAGCGCTCAA GGCCTGCTAC 
CGCGGCAACC GCTACCCCAC GCCGGACGAG AAGCGCCGCC TGGCCACACT CACCGGCCTG 
TCGCTCACGC AGGTCAGCAA CTGGTTCAAG AACCGGCGAC AGCGCGACCG GACCGGGGCC 
GGAGGCGGCG CGCCCTGCAA GAGCGAGTCT GATGGGAATC CCACGACTGA GGACGAGTCC 
AGCCGAAGTC CTGAGGACCT GGAGAGAGGG GCGGCCCCAG TGTCCGCCGA GGCCGCTGCC 
CAGGGCTCCA TATTCCTGGC AGGGACCGGC CCTCCCGCGC CTTGCCCGGC TTCCTCCTCC 
ATCCTGGTGA ACGGGAGCTT CCTGGCAGCC AGCGGCTCCC CAGCAGTGCT CCTCAACGGG 
GGCCCCGTCA TCATCAACGG CCTGGCCCTG GGCGAGGCCT CCAGCCTGGG CCCGCTGCTG 
CTCACTGGGG GCGGGGGTGC CCCTCCACCG CAGCCCAGCC CTCAGGGGGC CAGCGAGACC 
AAGACCTCTC TGGTCCTGGA CCCTCAGACA GGGGAGGTGC GGCTGGAGGA GGCTCAGTCG 
GAGGCCCCTG AGACCAAAGG GGCCCAGGTG GCTGCTCCGG GACCAGCCCT TGGAGAGGAG 
GTCCTGGGGC CCCTGGCCCA AGTGGTGCCT GGCCCCCCGA CGGCTGCCAC CTTTCCTCTG 
CCCCCGGGGC CAGTGCCTGC TGTGGCTGCC CCACAAGTGG TACCGCTCTC CCCACCCCCG 
GGGTATCCCA CGGGCCTGAG CCCCACCTCC CCACTATTGA ACCTGCCCCA GGTAGTACCC 
ACCTCACAGG TGGTGACCCT GCCCCAGGCT GTGGGGCCCC TGCAGCTGTT GGCAGCCGGG 
CCAGGCAGCC CTGTGAAGGT GGCAGCTGCA GCAGGCCCTG CCAATGTGCA CCTCATCAAC 
TCCGGGGTGG GCGTGACTGC CCTGCAGCTG CCTTCGGCCA CTGCCCCAGG AAACTTCCTC 
CTGGCCAACC CTGTGTCTGG CAGCCCCATC GTGACGGGTG TGGCCGTGCA GCAGGGCAAG 
ATCATCCTCA CCGCCACCTT CCCCACCAGC ATGCTCGTCT CCCAGGTCCT GCCGCCAGCC 
CCCGGCCTGG CCCTGCCACT GAAGCCAGAG ACGGCCATCT CCGTGCCTGA GGGAGGCCTC 
CCGGTGGCCC CCAGCCCTGC TCTCCCAGAG GCTCACGCCC TAGGCACCCT TTCTGCACAG 
CAGCCACCCC CCGCCGCTGC CACCACCTCC AGCACCAGCC TGCCCTTCTC CCCTGACTCC 
CCTGGCCTCC TGCCCAACTT CCCGGCGCCC CCACCAGAGG GGCTGATGTT GTCACCCGCG 
GCCGTGCCTG TCTGGTCAGC AGGGCTGGAA CTAAGCGCAG GAACAGAGGG GCTGCTGGAA 
GCGGAAAAGG GGCTGGGGAC ACAGGCCCCC CACACCGTGC TGAGGCTGCC AGACCCCGAC 
CCTGAGGGGC TGCTCCTGGG GGCCACCGCA GGGGGTGAGG TTGACGAGGG GTTGGAAGCT 
GAGGCCAAGG TTCTGACCCA GCTCCAGTCG GTGCCTGTGG AGGAGCCCTT GGAACTGTGA