CSTF2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CSTF2
Entrez Gene ID	1478
Full Name	cleavage stimulation factor subunit 2
Synonyms	CstF-64
General protein information	Preferred Names cleavage stimulation factor subunit 2 Names cleavage stimulation factor subunit 2 CF-1 64 kDa subunit CSTF 64 kDa subunit betaCstF-64 variant 2 cleavage stimulation factor 64 kDa subunit cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq22.1
Summary	This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq, Jul 2008].
Disorder MIM:	300907

mRNA and Protein(s)

mRNA	Protein	Name
NM_001306206.1	NP_001293135.1	cleavage stimulation factor subunit 2 isoform 1
NM_001325.2	NP_001316.1	cleavage stimulation factor subunit 2 isoform 2
NM_001306209.1	NP_001293138.1	cleavage stimulation factor subunit 2 isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	CSTF2	NP_001316.1
Canis lupus familiaris (dog)	CSTF2	XP_549135.2
Mus musculus (house mouse)	Cstf2	NP_573459.1
Rattus norvegicus (Norway rat)	Cstf2	NP_001124486.1
Xenopus tropicalis (tropical clawed frog)	cstf2	NP_001123707.1
Gallus gallus (chicken)	CSTF2	NP_001006433.1
Danio rerio (zebrafish)	cstf2	NP_956408.2
	CSTF2	XP_001089558.2
Danio rerio (zebrafish)	LOC100534697	XP_005173367.1
Bos taurus (cattle)	CSTF2	NP_777110.1
Pan troglodytes (chimpanzee)	CSTF2	XP_529072.3

Gene Ontology
Bibliography

Related articles in PubMed

Competitive regulation of alternative splicing and alternative polyadenylation by hnRNP H and CstF64 determines acetylcholinesterase isoforms.
Nazim M, Masuda A, Rahman MA, Nasrin F, Takeda JI, Ohe K, Ohkawara B, Ito M, Ohno K
Nucleic acids research45(3)1455-1468(2017 02)

CstF-64 and 3'-UTR cis-element determine Star-PAP specificity for target mRNA selection by excluding PAP?.
Kandala DT, Mohan N, A V, A P S, G R, Laishram RS
Nucleic acids research44(2)811-23(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

hnRNP H binds to two specific G-runs in exon 5a of ACHE and activates the distal alternative 3 splice site (ss) between exons 5a and 5b. Furthermore, hnRNP H competes for binding of CstF64 to the overlapping binding sites in exon 5a, and suppresses the selection of a cryptic polyadenylation site, which additionally ensures transcription of the distal 3 ss required for the generation of AChET isoform.
Title: Competitive regulation of alternative splicing and alternative polyadenylation by hnRNP H and CstF64 determines acetylcholinesterase isoforms.

CstF-64 is dispensable for the expression/3'-end processing of Star-PAP target mRNAs.CstF-64 and 3'-UTR cis-element determine Star-PAP specificity for target mRNA selection by excluding poly A polymerase.
Title: CstF-64 and 3'-UTR cis-element determine Star-PAP specificity for target mRNA selection by excluding PAP?.

CstF64 is central to the function of a heat-labile factor, composed of cleavage/polyadenylation specificity factor, symplekin, and cleavage stimulation factor 64 and appears to be at least partly responsible for its cell cycle regulation.
Title: CstF64: cell cycle regulation and functional role in 3' end processing of replication-dependent histone mRNAs.

CstF64, an essential polyadenylation factor, is a master regulator of 3'-UTR shortening across multiple tumour types.
Title: Dynamic analyses of alternative polyadenylation from RNA-seq reveal a 3'-UTR landscape across seven tumour types.

CstF64 and CstF64tau modulate one another's expression and play overlapping as well as distinct roles in regulating global alternative polyadenylation profiles.
Title: Overlapping and distinct functions of CstF64 and CstF64? in mammalian mRNA 3' processing.

The following CSTF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CSTF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26574	NM_001325.2 Latest version!	Homo sapiens cleavage stimulation factor subunit 2 (CSTF2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu57111	NM_001306206.1 Latest version!	Homo sapiens cleavage stimulation factor subunit 2 (CSTF2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu57110	NM_001306209.1 Latest version!	Homo sapiens cleavage stimulation factor subunit 2 (CSTF2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CSTF2 ( NM_001325.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316.1 Homo sapiens cleavage stimulation factor subunit 2 (CSTF2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26574
Clone ID Related Accession (Same CDS sequence)	NM_001325.2
Accession Version	NM_001325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	cleavage stimulation factor subunit 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z95327.1, Z83819.1, M85085.1 and AW016125.1. This sequence is a reference standard in the RefSeqGene project. On Sep 17, 2005 this sequence version replaced NM_001325.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.511489.1, SRR1803616.249036.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTT TGACTGTGAG AGACCCAGCG GTGGATCGTT CTCTACGTTC TGTGTTCGTG 
GGGAACATTC CTTATGAAGC TACTGAAGAG CAGTTGAAGG ACATCTTTTC TGAGGTTGGA 
CCTGTTGTTA GTTTCAGATT GGTATACGAT AGAGAGACAG GAAAGCCAAA GGGTTATGGC 
TTCTGTGAAT ACCAAGACCA AGAGACAGCA CTTAGTGCCA TGCGGAACCT GAATGGGCGC 
GAATTCAGTG GGAGAGCACT TCGAGTGGAC AATGCTGCCA GTGAAAAGAA CAAAGAAGAG 
CTGAAGAGCC TTGGCACTGG TGCCCCTGTC ATTGAGTCAC CTTATGGAGA GACCATCAGT 
CCTGAGGATG CCCCTGAGTC CATTAGCAAA GCAGTTGCCA GCCTTCCACC AGAGCAGATG 
TTTGAGCTGA TGAAACAAAT GAAGCTCTGT GTCCAGAATA GTCCCCAGGA GGCACGGAAC 
ATGTTACTTC AGAACCCTCA ACTGGCTTAT GCTTTGCTGC AAGCACAGGT AGTGATGAGA 
ATTGTGGATC CGGAAATTGC CCTGAAAATT CTGCATCGCC AGACAAATAT CCCAACGCTG 
ATTGCAGGCA ACCCTCAGCC AGTCCATGGT GCTGGGCCTG GCTCAGGATC CAATGTGTCA 
ATGAACCAGC AGAATCCTCA GGCCCCTCAG GCCCAGTCTT TGGGTGGAAT GCATGTCAAT 
GGCGCACCTC CTCTGATGCA AGCTTCTATG CAGGGTGGAG TTCCAGCACC AGGGCAAATG 
CCAGCTGCTG TCACAGGACC TGGCCCTGGT TCCTTAGCTC CTGGAGGAGG AATGCAGGCT 
CAGGTTGGAA TGCCAGGAAG TGGACCAGTG TCCATGGAAC GGGGGCAAGT GCCGATGCAA 
GACCCCAGAG CAGCTATGCA GCGGGGATCC TTGCCTGCGA ATGTCCCAAC CCCTCGAGGC 
TTGTTAGGAG ATGCTCCGAA TGATCCACGG GGAGGCACTT TACTTTCTGT AACTGGAGAG 
GTAGAGCCTA GAGGTTACTT GGGACCACCT CATCAGGGTC CACCCATGCA CCATGTCCCT 
GGCCATGAGA GCCGAGGACC ACCCCCACAT GAACTGAGGG GAGGGCCATT ACCCGAGCCC 
AGACCTCTAA TGGCAGAACC AAGAGGACCC ATGCTAGATC AGAGGGGTCC ACCCTTGGAT 
GGCAGAGGTG GAAGGGATCC CCGAGGAATA GATGCACGAG GGATGGAGGC CCGAGCCATG 
GAGGCAAGAG GGTTAGATGC CAGAGGATTA GAGGCCCGTG CAATGGAGGC CCGTGCGATG 
GAAGCTCGTG CAATGGAGGC CCGAGCGATG GAGGCCCGTG CAATGGAAGT CCGAGGGATG 
GAGGCCAGAG GCATGGATAC CAGAGGCCCA GTGCCTGGCC CCAGAGGACC TATACCTAGT 
GGAATGCAGG GTCCCAGTCC AATTAACATG GGGGCGGTTG TCCCCCAGGG ATCCAGACAG 
GTCCCAGTCA TGCAGGGAAC AGGAATGCAA GGAGCAAGTA TACAGGGTGG AAGCCAGCCT 
GGCGGCTTTA GTCCCGGGCA GAACCAAGTC ACTCCACAGG ATCATGAGAA GGCTGCTTTG 
ATTATGCAGG TTCTACAACT GACTGCAGAC CAGATTGCCA TGTTGCCTCC TGAGCAAAGG 
CAGAGTATCC TGATTTTAAA GGAACAAATA CAGAAATCCA CTGGAGCACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316.1
CDS	59..1792
Translation	MAGLTVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGAPVIESPYGETISPEDAPESISKAVASLPPEQMFELMKQMKLCVQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTLIAGNPQPVHGAGPGSGSNVSMNQQNPQAPQAQSLGGMHVNGAPPLMQASMQGGVPAPGQMPAAVTGPGPGSLAPGGGMQAQVGMPGSGPVSMERGQVPMQDPRAAMQRGSLPANVPTPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPPHQGPPMHHVPGHESRGPPPHELRGGPLPEPRPLMAEPRGPMLDQRGPPLDGRGGRDPRGIDARGMEARAMEARGLDARGLEARAMEARAMEARAMEARAMEARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINMGAVVPQGSRQVPVMQGTGMQGASIQGGSQPGGFSPGQNQVTPQDHEKAALIMQVLQLTADQIAMLPPEQRQSILILKEQIQKSTGAP

Target ORF information:

RefSeq Version	NM_001325.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cleavage stimulation factor subunit 2 (CSTF2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001325.2

ORF Insert Sequence:

ATGGCGGGTT TGACTGTGAG AGACCCAGCG GTGGATCGTT CTCTACGTTC TGTGTTCGTG 
GGGAACATTC CTTATGAAGC TACTGAAGAG CAGTTGAAGG ACATCTTTTC TGAGGTTGGA 
CCTGTTGTTA GTTTCAGATT GGTATACGAT AGAGAGACAG GAAAGCCAAA GGGTTATGGC 
TTCTGTGAAT ACCAAGACCA AGAGACAGCA CTTAGTGCCA TGCGGAACCT GAATGGGCGC 
GAATTCAGTG GGAGAGCACT TCGAGTGGAC AATGCTGCCA GTGAAAAGAA CAAAGAAGAG 
CTGAAGAGCC TTGGCACTGG TGCCCCTGTC ATTGAGTCAC CTTATGGAGA GACCATCAGT 
CCTGAGGATG CCCCTGAGTC CATTAGCAAA GCAGTTGCCA GCCTTCCACC AGAGCAGATG 
TTTGAGCTGA TGAAACAAAT GAAGCTCTGT GTCCAGAATA GTCCCCAGGA GGCACGGAAC 
ATGTTACTTC AGAACCCTCA ACTGGCTTAT GCTTTGCTGC AAGCACAGGT AGTGATGAGA 
ATTGTGGATC CGGAAATTGC CCTGAAAATT CTGCATCGCC AGACAAATAT CCCAACGCTG 
ATTGCAGGCA ACCCTCAGCC AGTCCATGGT GCTGGGCCTG GCTCAGGATC CAATGTGTCA 
ATGAACCAGC AGAATCCTCA GGCCCCTCAG GCCCAGTCTT TGGGTGGAAT GCATGTCAAT 
GGCGCACCTC CTCTGATGCA AGCTTCTATG CAGGGTGGAG TTCCAGCACC AGGGCAAATG 
CCAGCTGCTG TCACAGGACC TGGCCCTGGT TCCTTAGCTC CTGGAGGAGG AATGCAGGCT 
CAGGTTGGAA TGCCAGGAAG TGGACCAGTG TCCATGGAAC GGGGGCAAGT GCCGATGCAA 
GACCCCAGAG CAGCTATGCA GCGGGGATCC TTGCCTGCGA ATGTCCCAAC CCCTCGAGGC 
TTGTTAGGAG ATGCTCCGAA TGATCCACGG GGAGGCACTT TACTTTCTGT AACTGGAGAG 
GTAGAGCCTA GAGGTTACTT GGGACCACCT CATCAGGGTC CACCCATGCA CCATGTCCCT 
GGCCATGAGA GCCGAGGACC ACCCCCACAT GAACTGAGGG GAGGGCCATT ACCCGAGCCC 
AGACCTCTAA TGGCAGAACC AAGAGGACCC ATGCTAGATC AGAGGGGTCC ACCCTTGGAT 
GGCAGAGGTG GAAGGGATCC CCGAGGAATA GATGCACGAG GGATGGAGGC CCGAGCCATG 
GAGGCAAGAG GGTTAGATGC CAGAGGATTA GAGGCCCGTG CAATGGAGGC CCGTGCGATG 
GAAGCTCGTG CAATGGAGGC CCGAGCGATG GAGGCCCGTG CAATGGAAGT CCGAGGGATG 
GAGGCCAGAG GCATGGATAC CAGAGGCCCA GTGCCTGGCC CCAGAGGACC TATACCTAGT 
GGAATGCAGG GTCCCAGTCC AATTAACATG GGGGCGGTTG TCCCCCAGGG ATCCAGACAG 
GTCCCAGTCA TGCAGGGAAC AGGAATGCAA GGAGCAAGTA TACAGGGTGG AAGCCAGCCT 
GGCGGCTTTA GTCCCGGGCA GAACCAAGTC ACTCCACAGG ATCATGAGAA GGCTGCTTTG 
ATTATGCAGG TTCTACAACT GACTGCAGAC CAGATTGCCA TGTTGCCTCC TGAGCAAAGG 
CAGAGTATCC TGATTTTAAA GGAACAAATA CAGAAATCCA CTGGAGCACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSTF2 ( NM_001306206.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001293135.1 Homo sapiens cleavage stimulation factor subunit 2 (CSTF2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu57111
Clone ID Related Accession (Same CDS sequence)	NM_001306206.1
Accession Version	NM_001306206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	cleavage stimulation factor subunit 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX384743.2, EU616679.1, AK095684.1, AK300600.1 and AI826277.1. On Apr 16, 2015 this sequence version replaced XM_011530873.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.151447.1, SRR1660807.210798.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTT TGACTGTGAG AGACCCAGCG GTGGATCGTT CTCTACGTTC TGTGTTCGTG 
GGGAACATTC CTTATGAAGC TACTGAAGAG CAGTTGAAGG ACATCTTTTC TGAGGTTGGA 
CCTGTTGTTA GTTTCAGATT GGTATACGAT AGAGAGACAG GAAAGCCAAA GGGTTATGGC 
TTCTGTGAAT ACCAAGACCA AGAGACAGCA CTTAGTGCCA TGCGGAACCT GAATGGGCGC 
GAATTCAGTG GGAGAGCACT TCGAGTGGAC AATGCTGCCA GTGAAAAGAA CAAAGAAGAG 
CTGAAGAGCC TTGGCACTGG TGCCCCTGTC ATTGAGTCAC CTTATGGAGA GACCATCAGT 
CCTGAGGATG CCCCTGAGTC CATTAGCAAA GCAGTTGCCA GCCTTCCACC AGAGCAGATG 
TTTGAGCTGA TGAAACAAAT GAAGCTCTGT GTCCAGAATA GTCCCCAGGA GGCACGGAAC 
ATGTTACTTC AGAACCCTCA ACTGGCTTAT GCTTTGCTGC AAGCACAGGT AGTGATGAGA 
ATTGTGGATC CGGAAATTGC CCTGAAAATT CTGCATCGCC AGACAAATAT CCCAACGCTG 
ATTGCAGGCA ACCCTCAGCC AGTCCATGGT GCTGGGCCTG GCTCAGGATC CAATGTGTCA 
ATGAACCAGC AGAATCCTCA GGCCCCTCAG GCCCAGTCTT TGGGTGGAAT GCATGTCAAT 
GGCGCACCTC CTCTGATGCA AGCTTCTATG CAGGGTGGAG TTCCAGCACC AGGGCAAATG 
CCAGCTGCTG TCACAGGACC TGGCCCTGGT TCCTTAGCTC CTGGAGGAGG AATGCAGGCT 
CAGGTTGGAA TGCCAGGAAG TGGACCAGTG TCCATGGAAC GGGGGCAAGG AACCCTACAG 
CACTCGCCCG TGGGACCCGC CGGGCCTGCA TCAATTGAGC GAGTTCAAGT GCCGATGCAA 
GACCCCAGAG CAGCTATGCA GCGGGGATCC TTGCCTGCGA ATGTCCCAAC CCCTCGAGGC 
TTGTTAGGAG ATGCTCCGAA TGATCCACGG GGAGGCACTT TACTTTCTGT AACTGGAGAG 
GTAGAGCCTA GAGGTTACTT GGGACCACCT CATCAGGGTC CACCCATGCA CCATGTCCCT 
GGCCATGAGA GCCGAGGACC ACCCCCACAT GAACTGAGGG GAGGGCCATT ACCCGAGCCC 
AGACCTCTAA TGGCAGAACC AAGAGGACCC ATGCTAGATC AGAGGGGTCC ACCCTTGGAT 
GGCAGAGGTG GAAGGGATCC CCGAGGAATA GATGCACGAG GGATGGAGGC CCGAGCCATG 
GAGGCAAGAG GGTTAGATGC CAGAGGATTA GAGGCCCGTG CAATGGAGGC CCGTGCGATG 
GAAGCTCGTG CAATGGAGGC CCGAGCGATG GAGGCCCGTG CAATGGAAGT CCGAGGGATG 
GAGGCCAGAG GCATGGATAC CAGAGGCCCA GTGCCTGGCC CCAGAGGACC TATACCTAGT 
GGAATGCAGG GTCCCAGTCC AATTAACATG GGGGCGGTTG TCCCCCAGGG ATCCAGACAG 
GTCCCAGTCA TGCAGGGAAC AGGAATGCAA GGAGCAAGTA TACAGGGTGG AAGCCAGCCT 
GGCGGCTTTA GTCCCGGGCA GAACCAAGTC ACTCCACAGG ATCATGAGAA GGCTGCTTTG 
ATTATGCAGG TTCTACAACT GACTGCAGAC CAGATTGCCA TGTTGCCTCC TGAGCAAAGG 
CAGAGTATCC TGATTTTAAA GGAACAAATA CAGAAATCCA CTGGAGCACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293135.1
CDS	55..1848
Translation	MAGLTVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGAPVIESPYGETISPEDAPESISKAVASLPPEQMFELMKQMKLCVQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTLIAGNPQPVHGAGPGSGSNVSMNQQNPQAPQAQSLGGMHVNGAPPLMQASMQGGVPAPGQMPAAVTGPGPGSLAPGGGMQAQVGMPGSGPVSMERGQGTLQHSPVGPAGPASIERVQVPMQDPRAAMQRGSLPANVPTPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPPHQGPPMHHVPGHESRGPPPHELRGGPLPEPRPLMAEPRGPMLDQRGPPLDGRGGRDPRGIDARGMEARAMEARGLDARGLEARAMEARAMEARAMEARAMEARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINMGAVVPQGSRQVPVMQGTGMQGASIQGGSQPGGFSPGQNQVTPQDHEKAALIMQVLQLTADQIAMLPPEQRQSILILKEQIQKSTGAP

Target ORF information:

RefSeq Version	NM_001306206.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cleavage stimulation factor subunit 2 (CSTF2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306206.1

ORF Insert Sequence:

ATGGCGGGTT TGACTGTGAG AGACCCAGCG GTGGATCGTT CTCTACGTTC TGTGTTCGTG 
GGGAACATTC CTTATGAAGC TACTGAAGAG CAGTTGAAGG ACATCTTTTC TGAGGTTGGA 
CCTGTTGTTA GTTTCAGATT GGTATACGAT AGAGAGACAG GAAAGCCAAA GGGTTATGGC 
TTCTGTGAAT ACCAAGACCA AGAGACAGCA CTTAGTGCCA TGCGGAACCT GAATGGGCGC 
GAATTCAGTG GGAGAGCACT TCGAGTGGAC AATGCTGCCA GTGAAAAGAA CAAAGAAGAG 
CTGAAGAGCC TTGGCACTGG TGCCCCTGTC ATTGAGTCAC CTTATGGAGA GACCATCAGT 
CCTGAGGATG CCCCTGAGTC CATTAGCAAA GCAGTTGCCA GCCTTCCACC AGAGCAGATG 
TTTGAGCTGA TGAAACAAAT GAAGCTCTGT GTCCAGAATA GTCCCCAGGA GGCACGGAAC 
ATGTTACTTC AGAACCCTCA ACTGGCTTAT GCTTTGCTGC AAGCACAGGT AGTGATGAGA 
ATTGTGGATC CGGAAATTGC CCTGAAAATT CTGCATCGCC AGACAAATAT CCCAACGCTG 
ATTGCAGGCA ACCCTCAGCC AGTCCATGGT GCTGGGCCTG GCTCAGGATC CAATGTGTCA 
ATGAACCAGC AGAATCCTCA GGCCCCTCAG GCCCAGTCTT TGGGTGGAAT GCATGTCAAT 
GGCGCACCTC CTCTGATGCA AGCTTCTATG CAGGGTGGAG TTCCAGCACC AGGGCAAATG 
CCAGCTGCTG TCACAGGACC TGGCCCTGGT TCCTTAGCTC CTGGAGGAGG AATGCAGGCT 
CAGGTTGGAA TGCCAGGAAG TGGACCAGTG TCCATGGAAC GGGGGCAAGG AACCCTACAG 
CACTCGCCCG TGGGACCCGC CGGGCCTGCA TCAATTGAGC GAGTTCAAGT GCCGATGCAA 
GACCCCAGAG CAGCTATGCA GCGGGGATCC TTGCCTGCGA ATGTCCCAAC CCCTCGAGGC 
TTGTTAGGAG ATGCTCCGAA TGATCCACGG GGAGGCACTT TACTTTCTGT AACTGGAGAG 
GTAGAGCCTA GAGGTTACTT GGGACCACCT CATCAGGGTC CACCCATGCA CCATGTCCCT 
GGCCATGAGA GCCGAGGACC ACCCCCACAT GAACTGAGGG GAGGGCCATT ACCCGAGCCC 
AGACCTCTAA TGGCAGAACC AAGAGGACCC ATGCTAGATC AGAGGGGTCC ACCCTTGGAT 
GGCAGAGGTG GAAGGGATCC CCGAGGAATA GATGCACGAG GGATGGAGGC CCGAGCCATG 
GAGGCAAGAG GGTTAGATGC CAGAGGATTA GAGGCCCGTG CAATGGAGGC CCGTGCGATG 
GAAGCTCGTG CAATGGAGGC CCGAGCGATG GAGGCCCGTG CAATGGAAGT CCGAGGGATG 
GAGGCCAGAG GCATGGATAC CAGAGGCCCA GTGCCTGGCC CCAGAGGACC TATACCTAGT 
GGAATGCAGG GTCCCAGTCC AATTAACATG GGGGCGGTTG TCCCCCAGGG ATCCAGACAG 
GTCCCAGTCA TGCAGGGAAC AGGAATGCAA GGAGCAAGTA TACAGGGTGG AAGCCAGCCT 
GGCGGCTTTA GTCCCGGGCA GAACCAAGTC ACTCCACAGG ATCATGAGAA GGCTGCTTTG 
ATTATGCAGG TTCTACAACT GACTGCAGAC CAGATTGCCA TGTTGCCTCC TGAGCAAAGG 
CAGAGTATCC TGATTTTAAA GGAACAAATA CAGAAATCCA CTGGAGCACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CSTF2 ( NM_001306209.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001293138.1 Homo sapiens cleavage stimulation factor subunit 2 (CSTF2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu57110
Clone ID Related Accession (Same CDS sequence)	NM_001306209.1
Accession Version	NM_001306209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	cleavage stimulation factor subunit 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX384743.2, BC033135.1, AK300600.1 and AI826277.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.117266.1, BC033135.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTT TGACTGTGAG AGACCCAGCG GTGGATCGTT CTCTACGTTC TGTGTTCGTG 
GGGAACATTC CTTATGAAGC TACTGAAGAG CAGTTGAAGG ACATCTTTTC TGAGGTTGGA 
CCTGTTGTTA GTTTCAGATT GGTATACGAT AGAGAGACAG GAAAGCCAAA GGGTTATGGC 
TTCTGTGAAT ACCAAGACCA AGAGACAGCA CTTAGTGCCA TGCGGAACCT GAATGGGCGC 
GAATTCAGTG GGAGAGCACT TCGAGTGGAC AATGCTGCCA GTGAAAAGAA CAAAGAAGAG 
CTGAAGAGCC TTGGCACTGG TGCCCCTGTC ATTGAGTCAC CTTATGGAGA GACCATCAGT 
CCTGAGGATG CCCCTGAGTC CATTAGCAAA GCAGTTGCCA GCCTTCCACC AGAGCAGATG 
TTTGAGCTGA TGAAACAAAT GAAGCTCTGT GTCCAGAATA GTCCCCAGGA GGCACGGAAC 
ATGTTACTTC AGAACCCTCA ACTGGCTTAT GCTTTGCTGC AAGCACAGGT AGTGATGAGA 
ATTGTGGATC CGGAAATTGC CCTGAAAATT CTGCATCGCC AGACAAATAT CCCAACGCTG 
ATTGCAGGCA ACCCTCAGCC AGTCCATGGT GCTGGGCCTG GCTCAGGATC CAATGTGTCA 
ATGAACCAGC AGAATCCTCA GGCCCCTCAG GCCCAGTCTT TGGGTGGAGT TCCAGCACCA 
GGGCAAATGC CAGCTGCTGT CACAGGACCT GGCCCTGGTT CCTTAGCTCC TGGAGGAGGA 
ATGCAGGCTC AGGTTGGAAT GCCAGGAAGT GGACCAGTGT CCATGGAACG GGGGCAAGTG 
CCGATGCAAG ACCCCAGAGC AGCTATGCAG CGGGGATCCT TGCCTGCGAA TGTCCCAACC 
CCTCGAGGCT TGTTAGGAGA TGCTCCGAAT GATCCACGGG GAGGCACTTT ACTTTCTGTA 
ACTGGAGAGG TAGAGCCTAG AGGTTACTTG GGACCACCTC ATCAGGGTCC ACCCATGCAC 
CATGTCCCTG GCCATGAGAG CCGAGGACCA CCCCCACATG AACTGAGGGG AGGGCCATTA 
CCCGAGCCCA GACCTCTAAT GGCAGAACCA AGAGGACCCA TGCTAGATCA GAGGGGTCCA 
CCCTTGGATG GCAGAGGTGG AAGGGATCCC CGAGGAATAG ATGCACGAGG GATGGAGGCC 
CGAGCCATGG AGGCAAGAGG GTTAGATGCC AGAGGATTAG AGGCCCGTGC AATGGAGGCC 
CGTGCGATGG AAGCTCGTGC AATGGAGGCC CGAGCGATGG AGGCCCGTGC AATGGAAGTC 
CGAGGGATGG AGGCCAGAGG CATGGATACC AGAGGCCCAG TGCCTGGCCC CAGAGGACCT 
ATACCTAGTG GAATGCAGGG TCCCAGTCCA ATTAACATGG GGGCGGTTGT CCCCCAGGGA 
TCCAGACAGG TCCCAGTCAT GCAGGGAACA GGAATGCAAG GAGCAAGTAT ACAGGGTGGA 
AGCCAGCCTG GCGGCTTTAG TCCCGGGCAG AACCAAGTCA CTCCACAGGA TCATGAGAAG 
GCTGCTTTGA TTATGCAGGT TCTACAACTG ACTGCAGACC AGATTGCCAT GTTGCCTCCT 
GAGCAAAGGC AGAGTATCCT GATTTTAAAG GAACAAATAC AGAAATCCAC TGGAGCACCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293138.1
CDS	55..1737
Translation	MAGLTVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGAPVIESPYGETISPEDAPESISKAVASLPPEQMFELMKQMKLCVQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTLIAGNPQPVHGAGPGSGSNVSMNQQNPQAPQAQSLGGVPAPGQMPAAVTGPGPGSLAPGGGMQAQVGMPGSGPVSMERGQVPMQDPRAAMQRGSLPANVPTPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPPHQGPPMHHVPGHESRGPPPHELRGGPLPEPRPLMAEPRGPMLDQRGPPLDGRGGRDPRGIDARGMEARAMEARGLDARGLEARAMEARAMEARAMEARAMEARAMEVRGMEARGMDTRGPVPGPRGPIPSGMQGPSPINMGAVVPQGSRQVPVMQGTGMQGASIQGGSQPGGFSPGQNQVTPQDHEKAALIMQVLQLTADQIAMLPPEQRQSILILKEQIQKSTGAP

Target ORF information:

RefSeq Version	NM_001306209.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cleavage stimulation factor subunit 2 (CSTF2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306209.1

ORF Insert Sequence:

ATGGCGGGTT TGACTGTGAG AGACCCAGCG GTGGATCGTT CTCTACGTTC TGTGTTCGTG 
GGGAACATTC CTTATGAAGC TACTGAAGAG CAGTTGAAGG ACATCTTTTC TGAGGTTGGA 
CCTGTTGTTA GTTTCAGATT GGTATACGAT AGAGAGACAG GAAAGCCAAA GGGTTATGGC 
TTCTGTGAAT ACCAAGACCA AGAGACAGCA CTTAGTGCCA TGCGGAACCT GAATGGGCGC 
GAATTCAGTG GGAGAGCACT TCGAGTGGAC AATGCTGCCA GTGAAAAGAA CAAAGAAGAG 
CTGAAGAGCC TTGGCACTGG TGCCCCTGTC ATTGAGTCAC CTTATGGAGA GACCATCAGT 
CCTGAGGATG CCCCTGAGTC CATTAGCAAA GCAGTTGCCA GCCTTCCACC AGAGCAGATG 
TTTGAGCTGA TGAAACAAAT GAAGCTCTGT GTCCAGAATA GTCCCCAGGA GGCACGGAAC 
ATGTTACTTC AGAACCCTCA ACTGGCTTAT GCTTTGCTGC AAGCACAGGT AGTGATGAGA 
ATTGTGGATC CGGAAATTGC CCTGAAAATT CTGCATCGCC AGACAAATAT CCCAACGCTG 
ATTGCAGGCA ACCCTCAGCC AGTCCATGGT GCTGGGCCTG GCTCAGGATC CAATGTGTCA 
ATGAACCAGC AGAATCCTCA GGCCCCTCAG GCCCAGTCTT TGGGTGGAGT TCCAGCACCA 
GGGCAAATGC CAGCTGCTGT CACAGGACCT GGCCCTGGTT CCTTAGCTCC TGGAGGAGGA 
ATGCAGGCTC AGGTTGGAAT GCCAGGAAGT GGACCAGTGT CCATGGAACG GGGGCAAGTG 
CCGATGCAAG ACCCCAGAGC AGCTATGCAG CGGGGATCCT TGCCTGCGAA TGTCCCAACC 
CCTCGAGGCT TGTTAGGAGA TGCTCCGAAT GATCCACGGG GAGGCACTTT ACTTTCTGTA 
ACTGGAGAGG TAGAGCCTAG AGGTTACTTG GGACCACCTC ATCAGGGTCC ACCCATGCAC 
CATGTCCCTG GCCATGAGAG CCGAGGACCA CCCCCACATG AACTGAGGGG AGGGCCATTA 
CCCGAGCCCA GACCTCTAAT GGCAGAACCA AGAGGACCCA TGCTAGATCA GAGGGGTCCA 
CCCTTGGATG GCAGAGGTGG AAGGGATCCC CGAGGAATAG ATGCACGAGG GATGGAGGCC 
CGAGCCATGG AGGCAAGAGG GTTAGATGCC AGAGGATTAG AGGCCCGTGC AATGGAGGCC 
CGTGCGATGG AAGCTCGTGC AATGGAGGCC CGAGCGATGG AGGCCCGTGC AATGGAAGTC 
CGAGGGATGG AGGCCAGAGG CATGGATACC AGAGGCCCAG TGCCTGGCCC CAGAGGACCT 
ATACCTAGTG GAATGCAGGG TCCCAGTCCA ATTAACATGG GGGCGGTTGT CCCCCAGGGA 
TCCAGACAGG TCCCAGTCAT GCAGGGAACA GGAATGCAAG GAGCAAGTAT ACAGGGTGGA 
AGCCAGCCTG GCGGCTTTAG TCCCGGGCAG AACCAAGTCA CTCCACAGGA TCATGAGAAG 
GCTGCTTTGA TTATGCAGGT TCTACAACTG ACTGCAGACC AGATTGCCAT GTTGCCTCCT 
GAGCAAAGGC AGAGTATCCT GATTTTAAAG GAACAAATAC AGAAATCCAC TGGAGCACCT 
TGA