PIWIL4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIWIL4
Entrez Gene ID	143689
Full Name	piwi like RNA-mediated gene silencing 4
Synonyms	HIWI2,MIWI2
General protein information	Preferred Names piwi like RNA-mediated gene silencing 4 Names piwi-like protein 4 piwi-like 4 testis tissue sperm-binding protein Li 85P
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q21
Summary	PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008].
Disorder MIM:	610315

mRNA and Protein(s)

mRNA	Protein	Name
NM_152431.2	NP_689644.2	piwi-like protein 4
NM_152431.3	NP_689644.2	piwi-like protein 4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Piwil4	NP_808573.2
Canis lupus familiaris (dog)	PIWIL4	XP_542241.3
Bos taurus (cattle)	PIWIL4	XP_002693007.1
Homo sapiens (human)	PIWIL4	NP_689644.2
Xenopus tropicalis (tropical clawed frog)	piwil4	XP_002939761.2
Pan troglodytes (chimpanzee)	PIWIL4	XP_508702.2
Rattus norvegicus (Norway rat)	Piwil4	NP_001258062.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of Piwi protein MIWI2 defines a distinct population of multiciliated cells.
Wasserman GA, Szymaniak AD, Hinds AC, Yamamoto K, Kamata H, Smith NM, Hilliard KL, Carrieri C, Labadorf AT, Quinton LJ, Ai X, Varelas X, Chen F, Mizgerd JP, Fine A, O'Carroll D, Jones MR
The Journal of clinical investigation127(10)3866-3876(2017 Oct)

Possible role of HIWI2 in modulating tight junction proteins in retinal pigment epithelial cells through Akt signaling pathway.
Sivagurunathan S, Palanisamy K, Arunachalam JP, Chidambaram S
Molecular and cellular biochemistry427(1-2)145-156(2017 Mar)

PIWI-like protein, HIWI2 is aberrantly expressed in retinoblastoma cells and affects cell-cycle potentially through OTX2.
Sivagurunathan S, Arunachalam JP, Chidambaram S
Cellular & molecular biology letters2217(2017)

Co-expression of Piwil2/Piwil4 in nucleus indicates poor prognosis of hepatocellular carcinoma.
Zeng G, Zhang D, Liu X, Kang Q, Fu Y, Tang B, Guo W, Zhang Y, Wei G, He D
Oncotarget8(3)4607-4617(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data delineate MIWI2-dependent functions outside of the germline and demonstrate the presence of distinct subsets of airway multiciliated cells that can be discriminated by MIWI2 expression.
Title: Expression of Piwi protein MIWI2 defines a distinct population of multiciliated cells.

study demonstrates the presence of PIWI-like proteins in somatic cells and the possible role of HIWI2 in preserving the functional integrity of epithelial cells probably by modulating the phosphorylation status of Akt.
Title: Possible role of HIWI2 in modulating tight junction proteins in retinal pigment epithelial cells through Akt signaling pathway.

HIWI2 is aberrantly expressed in cells of the Retinoblastoma (RB) line (Y79) and silencing of HIWI2 downregulates OTX2, suggesting that HIWI2 might affects cell cycle and plays a role in the progression of RB.
Title: PIWI-like protein, HIWI2 is aberrantly expressed in retinoblastoma cells and affects cell-cycle potentially through OTX2.

MiR-384 functioned as tumor suppressor by decreasing PIWIL4 in glioma cell lines.
Title: CRNDE Promotes Malignant Progression of Glioma by Attenuating miR-384/PIWIL4/STAT3 Axis.

PIWIL2 and PIWIL4 genes expression in synovial fibroblasts of the rheumatoid arthritis patients.PIWIL4 does not regulate methylation or expression of LINE-1.
Title: Expression and Regulation of PIWIL-Proteins and PIWI-Interacting RNAs in Rheumatoid Arthritis.

The following PIWIL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIWIL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24145	NM_152431.3 Latest version!	Homo sapiens piwi like RNA-mediated gene silencing 4 (PIWIL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
View Old Accession Versions >>
OHu24145	NM_152431.2	Homo sapiens piwi like RNA-mediated gene silencing 4 (PIWIL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIWIL4 ( NM_152431.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_689644.2 Homo sapiens piwi like RNA-mediated gene silencing 4 (PIWIL4), mRNA.

Price & Availability↑

CloneID	OHu24145
Clone ID Related Accession (Same CDS sequence)	NM_152431.2 , NM_152431.3
Accession Version	NM_152431.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Homo sapiens(human)
Product	piwi-like protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB091271.1, BC031060.1 and AB079366.1. On Jan 24, 2009 this sequence version replaced NM_152431.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005708.1, AB079366.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGGAA GAGCCCGAGT GAAGGCCAGA GGCATCGCCC GCAGCCCCAG TGCCACAGAA 
GTGGGGCGCA TCCAAGCCTC GCCATTGCCT AGATCTGTTG ATCTTAGTAA CAATGAAGCA 
TCCTCTAGCA ATGGCTTCTT GGGAACAAGC AGGATCTCAA CCAACGATAA ATATGGGATA 
TCTTCTGGTG ATGCTGGAAG TACCTTCATG GAAAGAGGTG TGAAAAACAA ACAGGACTTT 
ATGGATTTGA GTATCTGTAC CAGAGAAAAA TTGGCACATG TGAGAAATTG TAAAACAGGT 
TCCAGTGGAA TACCTGTGAA ACTGGTTACA AACCTCTTTA ACTTAGATTT TCCCCAAGAC 
TGGCAGCTAT ACCAGTACCA TGTGACATAT ATTCCAGATT TAGCATCTAG AAGGCTGAGA 
ATTGCTTTAC TTTATAGTCA TAGTGAACTT TCCAACAAAG CAAAAGCATT CGACGGTGCC 
ATCCTTTTTC TGTCACAAAA GCTAGAAGAA AAGGTCACAG AGTTGTCAAG TGAAACTCAA 
AGAGGTGAGA CTATAAAGAT GACTATCACC CTGAAGAGGG AGCTGCCATC AAGTTCTCCC 
GTGTGCATCC AGGTCTTCAA TATCATCTTC AGAAAGATCC TCAAAAAGTT GTCCATGTAC 
CAAATTGGAC GGAACTTCTA TAATCCTTCA GAGCCAATGG AAATTCCCCA GCACAAATTA 
TCCCTTTGGC CTGGGTTTGC CATTTCTGTG TCATATTTTG AAAGGAAGCT CCTGTTTAGT 
GCTGATGTGA GTTACAAAGT CCTCCGGAAT GAGACGGTTC TGGAATTCAT GACTGCTCTC 
TGTCAAAGAA CTGGCTTGTC CTGTTTCACC CAGACGTGTG AGAAGCAGCT AATAGGGCTC 
ATTGTCCTTA CAAGATACAA TAACAGAACC TACTCCATTG ATGACATTGA CTGGTCAGTG 
AAGCCCACAC ACACCTTTCA GAAGCGGGAT GGCACCGAGA TCACCTATGT GGATTACTAC 
AAGCAGCAGT ATGATATTAC TGTATCGGAC CTGAATCAGC CCATGCTTGT TAGTCTGTTA 
AAGAAGAAGA GAAATGACAA CAGTGAGGCT CAGCTCGCCC ACCTGATACC TGAGCTCTGC 
TTTCTAACAG GGCTGACTGA CCAGGCAACA TCTGATTTCC AGCTGATGAA GGCTGTGGCT 
GAAAAGACAC GTCTCAGTCC TTCAGGCCGG CAGCAGCGCC TGGCCAGGCT TGTGGACAAC 
ATCCAGAGGA ATACCAATGC TCGCTTTGAA CTAGAGACCT GGGGACTGCA TTTTGGAAGC 
CAGATATCTC TGACTGGCCG GATTGTGCCT TCAGAAAAAA TATTAATGCA AGACCACATA 
TGTCAACCTG TGTCTGCTGC TGACTGGTCC AAGGATATTC GAACTTGCAA GATTTTAAAT 
GCACAGTCTT TGAATACCTG GTTGATTTTA TGTAGCGACA GAACTGAATA TGTTGCCGAG 
AGCTTTCTGA ACTGCTTGAG AAGAGTTGCA GGTTCCATGG GATTTAATGT GGACTACCCC 
AAAATCATAA AAGTACAAGA AAATCCAGCT GCATTTGTTA GAGCTATACA GCAATATGTT 
GATCCTGATG TTCAGCTGGT AATGTGCATT CTGCCTTCTA ATCAGAAGAC CTATTATGAT 
TCCATTAAAA AATATTTGAG CTCAGACTGC CCAGTCCCAA GCCAATGTGT GCTTGCTCGG 
ACCTTGAATA AACAGGGCAT GATGATGAGT ATCGCCACCA AGATCGCTAT GCAGATGACT 
TGCAAGCTCG GAGGCGAGCT GTGGGCTGTG GAAATACCTT TAAAGTCCCT GATGGTGGTC 
GGTATTGATG TCTGTAAAGA TGCACTCAGC AAGGACGTGA TGGTTGTTGG ATGCGTGGCC 
AGTGTTAACC CCAGAATCAC CAGGTGGTTT TCCCGCTGTA TCCTTCAGAG AACAATGACT 
GATGTTGCAG ATTGCTTGAA AGTTTTCATG ACTGGAGCAC TCAACAAATG GTACAAGTAC 
AATCATGATT TGCCAGCACG GATAATTGTG TACCGTGCTG GTGTAGGGGA TGGTCAGCTG 
AAAACACTTA TTGAATATGA AGTCCCACAG CTGCTGAGCA GTGTGGCAGA ATCCAGCTCA 
AATACCAGCT CAAGACTGTC GGTGATTGTG GTCAGGAAGA AGTGCATGCC ACGATTCTTT 
ACCGAAATGA ACCGCACTGT ACAGAACCCC CCACTTGGCA CTGTTGTGGA TTCAGAAGCA 
ACACGTAACG AATGGTATGA CTTTTATCTG ATCAGCCAGG TGGCCTGCCG GGGAACTGTT 
AGTCCTACCT ACTATAATGT CATCTATGAT GACAACGGCT TGAAGCCCGA CCATATGCAG 
AGACTTACAT TCAAATTGTG CCACCTGTAC TACAACTGGC CGGGCATAGT CAGTGTCCCA 
GCACCATGTC AGTATGCTCA CAAGCTGACC TTTCTGGTGG CACAAAGCAT TCATAAAGAA 
CCCAGTCTGG AATTAGCCAA CCATCTCTTC TACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689644.2
CDS	212..2770
Translation	MSGRARVKARGIARSPSATEVGRIQASPLPRSVDLSNNEASSSNGFLGTSRISTNDKYGISSGDAGSTFMERGVKNKQDFMDLSICTREKLAHVRNCKTGSSGIPVKLVTNLFNLDFPQDWQLYQYHVTYIPDLASRRLRIALLYSHSELSNKAKAFDGAILFLSQKLEEKVTELSSETQRGETIKMTITLKRELPSSSPVCIQVFNIIFRKILKKLSMYQIGRNFYNPSEPMEIPQHKLSLWPGFAISVSYFERKLLFSADVSYKVLRNETVLEFMTALCQRTGLSCFTQTCEKQLIGLIVLTRYNNRTYSIDDIDWSVKPTHTFQKRDGTEITYVDYYKQQYDITVSDLNQPMLVSLLKKKRNDNSEAQLAHLIPELCFLTGLTDQATSDFQLMKAVAEKTRLSPSGRQQRLARLVDNIQRNTNARFELETWGLHFGSQISLTGRIVPSEKILMQDHICQPVSAADWSKDIRTCKILNAQSLNTWLILCSDRTEYVAESFLNCLRRVAGSMGFNVDYPKIIKVQENPAAFVRAIQQYVDPDVQLVMCILPSNQKTYYDSIKKYLSSDCPVPSQCVLARTLNKQGMMMSIATKIAMQMTCKLGGELWAVEIPLKSLMVVGIDVCKDALSKDVMVVGCVASVNPRITRWFSRCILQRTMTDVADCLKVFMTGALNKWYKYNHDLPARIIVYRAGVGDGQLKTLIEYEVPQLLSSVAESSSNTSSRLSVIVVRKKCMPRFFTEMNRTVQNPPLGTVVDSEATRNEWYDFYLISQVACRGTVSPTYYNVIYDDNGLKPDHMQRLTFKLCHLYYNWPGIVSVPAPCQYAHKLTFLVAQSIHKEPSLELANHLFYL

Target ORF information:

RefSeq Version	NM_152431.2
Organism	Homo sapiens(human)
Definition	Homo sapiens piwi like RNA-mediated gene silencing 4 (PIWIL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152431.2

ORF Insert Sequence:

ATGAGTGGAA GAGCCCGAGT GAAGGCCAGA GGCATCGCCC GCAGCCCCAG TGCCACAGAA 
GTGGGGCGCA TCCAAGCCTC GCCATTGCCT AGATCTGTTG ATCTTAGTAA CAATGAAGCA 
TCCTCTAGCA ATGGCTTCTT GGGAACAAGC AGGATCTCAA CCAACGATAA ATATGGGATA 
TCTTCTGGTG ATGCTGGAAG TACCTTCATG GAAAGAGGTG TGAAAAACAA ACAGGACTTT 
ATGGATTTGA GTATCTGTAC CAGAGAAAAA TTGGCACATG TGAGAAATTG TAAAACAGGT 
TCCAGTGGAA TACCTGTGAA ACTGGTTACA AACCTCTTTA ACTTAGATTT TCCCCAAGAC 
TGGCAGCTAT ACCAGTACCA TGTGACATAT ATTCCAGATT TAGCATCTAG AAGGCTGAGA 
ATTGCTTTAC TTTATAGTCA TAGTGAACTT TCCAACAAAG CAAAAGCATT CGACGGTGCC 
ATCCTTTTTC TGTCACAAAA GCTAGAAGAA AAGGTCACAG AGTTGTCAAG TGAAACTCAA 
AGAGGTGAGA CTATAAAGAT GACTATCACC CTGAAGAGGG AGCTGCCATC AAGTTCTCCC 
GTGTGCATCC AGGTCTTCAA TATCATCTTC AGAAAGATCC TCAAAAAGTT GTCCATGTAC 
CAAATTGGAC GGAACTTCTA TAATCCTTCA GAGCCAATGG AAATTCCCCA GCACAAATTA 
TCCCTTTGGC CTGGGTTTGC CATTTCTGTG TCATATTTTG AAAGGAAGCT CCTGTTTAGT 
GCTGATGTGA GTTACAAAGT CCTCCGGAAT GAGACGGTTC TGGAATTCAT GACTGCTCTC 
TGTCAAAGAA CTGGCTTGTC CTGTTTCACC CAGACGTGTG AGAAGCAGCT AATAGGGCTC 
ATTGTCCTTA CAAGATACAA TAACAGAACC TACTCCATTG ATGACATTGA CTGGTCAGTG 
AAGCCCACAC ACACCTTTCA GAAGCGGGAT GGCACCGAGA TCACCTATGT GGATTACTAC 
AAGCAGCAGT ATGATATTAC TGTATCGGAC CTGAATCAGC CCATGCTTGT TAGTCTGTTA 
AAGAAGAAGA GAAATGACAA CAGTGAGGCT CAGCTCGCCC ACCTGATACC TGAGCTCTGC 
TTTCTAACAG GGCTGACTGA CCAGGCAACA TCTGATTTCC AGCTGATGAA GGCTGTGGCT 
GAAAAGACAC GTCTCAGTCC TTCAGGCCGG CAGCAGCGCC TGGCCAGGCT TGTGGACAAC 
ATCCAGAGGA ATACCAATGC TCGCTTTGAA CTAGAGACCT GGGGACTGCA TTTTGGAAGC 
CAGATATCTC TGACTGGCCG GATTGTGCCT TCAGAAAAAA TATTAATGCA AGACCACATA 
TGTCAACCTG TGTCTGCTGC TGACTGGTCC AAGGATATTC GAACTTGCAA GATTTTAAAT 
GCACAGTCTT TGAATACCTG GTTGATTTTA TGTAGCGACA GAACTGAATA TGTTGCCGAG 
AGCTTTCTGA ACTGCTTGAG AAGAGTTGCA GGTTCCATGG GATTTAATGT GGACTACCCC 
AAAATCATAA AAGTACAAGA AAATCCAGCT GCATTTGTTA GAGCTATACA GCAATATGTT 
GATCCTGATG TTCAGCTGGT AATGTGCATT CTGCCTTCTA ATCAGAAGAC CTATTATGAT 
TCCATTAAAA AATATTTGAG CTCAGACTGC CCAGTCCCAA GCCAATGTGT GCTTGCTCGG 
ACCTTGAATA AACAGGGCAT GATGATGAGT ATCGCCACCA AGATCGCTAT GCAGATGACT 
TGCAAGCTCG GAGGCGAGCT GTGGGCTGTG GAAATACCTT TAAAGTCCCT GATGGTGGTC 
GGTATTGATG TCTGTAAAGA TGCACTCAGC AAGGACGTGA TGGTTGTTGG ATGCGTGGCC 
AGTGTTAACC CCAGAATCAC CAGGTGGTTT TCCCGCTGTA TCCTTCAGAG AACAATGACT 
GATGTTGCAG ATTGCTTGAA AGTTTTCATG ACTGGAGCAC TCAACAAATG GTACAAGTAC 
AATCATGATT TGCCAGCACG GATAATTGTG TACCGTGCTG GTGTAGGGGA TGGTCAGCTG 
AAAACACTTA TTGAATATGA AGTCCCACAG CTGCTGAGCA GTGTGGCAGA ATCCAGCTCA 
AATACCAGCT CAAGACTGTC GGTGATTGTG GTCAGGAAGA AGTGCATGCC ACGATTCTTT 
ACCGAAATGA ACCGCACTGT ACAGAACCCC CCACTTGGCA CTGTTGTGGA TTCAGAAGCA 
ACACGTAACG AATGGTATGA CTTTTATCTG ATCAGCCAGG TGGCCTGCCG GGGAACTGTT 
AGTCCTACCT ACTATAATGT CATCTATGAT GACAACGGCT TGAAGCCCGA CCATATGCAG 
AGACTTACAT TCAAATTGTG CCACCTGTAC TACAACTGGC CGGGCATAGT CAGTGTCCCA 
GCACCATGTC AGTATGCTCA CAAGCTGACC TTTCTGGTGG CACAAAGCAT TCATAAAGAA 
CCCAGTCTGG AATTAGCCAA CCATCTCTTC TACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIWIL4 ( NM_152431.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689644.2 Homo sapiens piwi like RNA-mediated gene silencing 4 (PIWIL4), mRNA.

Price & Availability↑

CloneID	OHu24145
Clone ID Related Accession (Same CDS sequence)	NM_152431.2 , NM_152431.3
Accession Version	NM_152431.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	piwi-like protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB091271.1, BC031060.1 and AB079366.1. On Nov 22, 2018 this sequence version replaced NM_152431.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005708.1, AB079366.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000299001.11/ ENSP00000299001.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTGGAA GAGCCCGAGT GAAGGCCAGA GGCATCGCCC GCAGCCCCAG TGCCACAGAA 
GTGGGGCGCA TCCAAGCCTC GCCATTGCCT AGATCTGTTG ATCTTAGTAA CAATGAAGCA 
TCCTCTAGCA ATGGCTTCTT GGGAACAAGC AGGATCTCAA CCAACGATAA ATATGGGATA 
TCTTCTGGTG ATGCTGGAAG TACCTTCATG GAAAGAGGTG TGAAAAACAA ACAGGACTTT 
ATGGATTTGA GTATCTGTAC CAGAGAAAAA TTGGCACATG TGAGAAATTG TAAAACAGGT 
TCCAGTGGAA TACCTGTGAA ACTGGTTACA AACCTCTTTA ACTTAGATTT TCCCCAAGAC 
TGGCAGCTAT ACCAGTACCA TGTGACATAT ATTCCAGATT TAGCATCTAG AAGGCTGAGA 
ATTGCTTTAC TTTATAGTCA TAGTGAACTT TCCAACAAAG CAAAAGCATT CGACGGTGCC 
ATCCTTTTTC TGTCACAAAA GCTAGAAGAA AAGGTCACAG AGTTGTCAAG TGAAACTCAA 
AGAGGTGAGA CTATAAAGAT GACTATCACC CTGAAGAGGG AGCTGCCATC AAGTTCTCCC 
GTGTGCATCC AGGTCTTCAA TATCATCTTC AGAAAGATCC TCAAAAAGTT GTCCATGTAC 
CAAATTGGAC GGAACTTCTA TAATCCTTCA GAGCCAATGG AAATTCCCCA GCACAAATTA 
TCCCTTTGGC CTGGGTTTGC CATTTCTGTG TCATATTTTG AAAGGAAGCT CCTGTTTAGT 
GCTGATGTGA GTTACAAAGT CCTCCGGAAT GAGACGGTTC TGGAATTCAT GACTGCTCTC 
TGTCAAAGAA CTGGCTTGTC CTGTTTCACC CAGACGTGTG AGAAGCAGCT AATAGGGCTC 
ATTGTCCTTA CAAGATACAA TAACAGAACC TACTCCATTG ATGACATTGA CTGGTCAGTG 
AAGCCCACAC ACACCTTTCA GAAGCGGGAT GGCACCGAGA TCACCTATGT GGATTACTAC 
AAGCAGCAGT ATGATATTAC TGTATCGGAC CTGAATCAGC CCATGCTTGT TAGTCTGTTA 
AAGAAGAAGA GAAATGACAA CAGTGAGGCT CAGCTCGCCC ACCTGATACC TGAGCTCTGC 
TTTCTAACAG GGCTGACTGA CCAGGCAACA TCTGATTTCC AGCTGATGAA GGCTGTGGCT 
GAAAAGACAC GTCTCAGTCC TTCAGGCCGG CAGCAGCGCC TGGCCAGGCT TGTGGACAAC 
ATCCAGAGGA ATACCAATGC TCGCTTTGAA CTAGAGACCT GGGGACTGCA TTTTGGAAGC 
CAGATATCTC TGACTGGCCG GATTGTGCCT TCAGAAAAAA TATTAATGCA AGACCACATA 
TGTCAACCTG TGTCTGCTGC TGACTGGTCC AAGGATATTC GAACTTGCAA GATTTTAAAT 
GCACAGTCTT TGAATACCTG GTTGATTTTA TGTAGCGACA GAACTGAATA TGTTGCCGAG 
AGCTTTCTGA ACTGCTTGAG AAGAGTTGCA GGTTCCATGG GATTTAATGT GGACTACCCC 
AAAATCATAA AAGTACAAGA AAATCCAGCT GCATTTGTTA GAGCTATACA GCAATATGTT 
GATCCTGATG TTCAGCTGGT AATGTGCATT CTGCCTTCTA ATCAGAAGAC CTATTATGAT 
TCCATTAAAA AATATTTGAG CTCAGACTGC CCAGTCCCAA GCCAATGTGT GCTTGCTCGG 
ACCTTGAATA AACAGGGCAT GATGATGAGT ATCGCCACCA AGATCGCTAT GCAGATGACT 
TGCAAGCTCG GAGGCGAGCT GTGGGCTGTG GAAATACCTT TAAAGTCCCT GATGGTGGTC 
GGTATTGATG TCTGTAAAGA TGCACTCAGC AAGGACGTGA TGGTTGTTGG ATGCGTGGCC 
AGTGTTAACC CCAGAATCAC CAGGTGGTTT TCCCGCTGTA TCCTTCAGAG AACAATGACT 
GATGTTGCAG ATTGCTTGAA AGTTTTCATG ACTGGAGCAC TCAACAAATG GTACAAGTAC 
AATCATGATT TGCCAGCACG GATAATTGTG TACCGTGCTG GTGTAGGGGA TGGTCAGCTG 
AAAACACTTA TTGAATATGA AGTCCCACAG CTGCTGAGCA GTGTGGCAGA ATCCAGCTCA 
AATACCAGCT CAAGACTGTC GGTGATTGTG GTCAGGAAGA AGTGCATGCC ACGATTCTTT 
ACCGAAATGA ACCGCACTGT ACAGAACCCC CCACTTGGCA CTGTTGTGGA TTCAGAAGCA 
ACACGTAACG AATGGTATGA CTTTTATCTG ATCAGCCAGG TGGCCTGCCG GGGAACTGTT 
AGTCCTACCT ACTATAATGT CATCTATGAT GACAACGGCT TGAAGCCCGA CCATATGCAG 
AGACTTACAT TCAAATTGTG CCACCTGTAC TACAACTGGC CGGGCATAGT CAGTGTCCCA 
GCACCATGTC AGTATGCTCA CAAGCTGACC TTTCTGGTGG CACAAAGCAT TCATAAAGAA 
CCCAGTCTGG AATTAGCCAA CCATCTCTTC TACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689644.2
CDS	152..2710
Translation	MSGRARVKARGIARSPSATEVGRIQASPLPRSVDLSNNEASSSNGFLGTSRISTNDKYGISSGDAGSTFMERGVKNKQDFMDLSICTREKLAHVRNCKTGSSGIPVKLVTNLFNLDFPQDWQLYQYHVTYIPDLASRRLRIALLYSHSELSNKAKAFDGAILFLSQKLEEKVTELSSETQRGETIKMTITLKRELPSSSPVCIQVFNIIFRKILKKLSMYQIGRNFYNPSEPMEIPQHKLSLWPGFAISVSYFERKLLFSADVSYKVLRNETVLEFMTALCQRTGLSCFTQTCEKQLIGLIVLTRYNNRTYSIDDIDWSVKPTHTFQKRDGTEITYVDYYKQQYDITVSDLNQPMLVSLLKKKRNDNSEAQLAHLIPELCFLTGLTDQATSDFQLMKAVAEKTRLSPSGRQQRLARLVDNIQRNTNARFELETWGLHFGSQISLTGRIVPSEKILMQDHICQPVSAADWSKDIRTCKILNAQSLNTWLILCSDRTEYVAESFLNCLRRVAGSMGFNVDYPKIIKVQENPAAFVRAIQQYVDPDVQLVMCILPSNQKTYYDSIKKYLSSDCPVPSQCVLARTLNKQGMMMSIATKIAMQMTCKLGGELWAVEIPLKSLMVVGIDVCKDALSKDVMVVGCVASVNPRITRWFSRCILQRTMTDVADCLKVFMTGALNKWYKYNHDLPARIIVYRAGVGDGQLKTLIEYEVPQLLSSVAESSSNTSSRLSVIVVRKKCMPRFFTEMNRTVQNPPLGTVVDSEATRNEWYDFYLISQVACRGTVSPTYYNVIYDDNGLKPDHMQRLTFKLCHLYYNWPGIVSVPAPCQYAHKLTFLVAQSIHKEPSLELANHLFYL

Target ORF information:

RefSeq Version	NM_152431.3
Organism	Homo sapiens(human)
Definition	Homo sapiens piwi like RNA-mediated gene silencing 4 (PIWIL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152431.3

ORF Insert Sequence:

ATGAGTGGAA GAGCCCGAGT GAAGGCCAGA GGCATCGCCC GCAGCCCCAG TGCCACAGAA 
GTGGGGCGCA TCCAAGCCTC GCCATTGCCT AGATCTGTTG ATCTTAGTAA CAATGAAGCA 
TCCTCTAGCA ATGGCTTCTT GGGAACAAGC AGGATCTCAA CCAACGATAA ATATGGGATA 
TCTTCTGGTG ATGCTGGAAG TACCTTCATG GAAAGAGGTG TGAAAAACAA ACAGGACTTT 
ATGGATTTGA GTATCTGTAC CAGAGAAAAA TTGGCACATG TGAGAAATTG TAAAACAGGT 
TCCAGTGGAA TACCTGTGAA ACTGGTTACA AACCTCTTTA ACTTAGATTT TCCCCAAGAC 
TGGCAGCTAT ACCAGTACCA TGTGACATAT ATTCCAGATT TAGCATCTAG AAGGCTGAGA 
ATTGCTTTAC TTTATAGTCA TAGTGAACTT TCCAACAAAG CAAAAGCATT CGACGGTGCC 
ATCCTTTTTC TGTCACAAAA GCTAGAAGAA AAGGTCACAG AGTTGTCAAG TGAAACTCAA 
AGAGGTGAGA CTATAAAGAT GACTATCACC CTGAAGAGGG AGCTGCCATC AAGTTCTCCC 
GTGTGCATCC AGGTCTTCAA TATCATCTTC AGAAAGATCC TCAAAAAGTT GTCCATGTAC 
CAAATTGGAC GGAACTTCTA TAATCCTTCA GAGCCAATGG AAATTCCCCA GCACAAATTA 
TCCCTTTGGC CTGGGTTTGC CATTTCTGTG TCATATTTTG AAAGGAAGCT CCTGTTTAGT 
GCTGATGTGA GTTACAAAGT CCTCCGGAAT GAGACGGTTC TGGAATTCAT GACTGCTCTC 
TGTCAAAGAA CTGGCTTGTC CTGTTTCACC CAGACGTGTG AGAAGCAGCT AATAGGGCTC 
ATTGTCCTTA CAAGATACAA TAACAGAACC TACTCCATTG ATGACATTGA CTGGTCAGTG 
AAGCCCACAC ACACCTTTCA GAAGCGGGAT GGCACCGAGA TCACCTATGT GGATTACTAC 
AAGCAGCAGT ATGATATTAC TGTATCGGAC CTGAATCAGC CCATGCTTGT TAGTCTGTTA 
AAGAAGAAGA GAAATGACAA CAGTGAGGCT CAGCTCGCCC ACCTGATACC TGAGCTCTGC 
TTTCTAACAG GGCTGACTGA CCAGGCAACA TCTGATTTCC AGCTGATGAA GGCTGTGGCT 
GAAAAGACAC GTCTCAGTCC TTCAGGCCGG CAGCAGCGCC TGGCCAGGCT TGTGGACAAC 
ATCCAGAGGA ATACCAATGC TCGCTTTGAA CTAGAGACCT GGGGACTGCA TTTTGGAAGC 
CAGATATCTC TGACTGGCCG GATTGTGCCT TCAGAAAAAA TATTAATGCA AGACCACATA 
TGTCAACCTG TGTCTGCTGC TGACTGGTCC AAGGATATTC GAACTTGCAA GATTTTAAAT 
GCACAGTCTT TGAATACCTG GTTGATTTTA TGTAGCGACA GAACTGAATA TGTTGCCGAG 
AGCTTTCTGA ACTGCTTGAG AAGAGTTGCA GGTTCCATGG GATTTAATGT GGACTACCCC 
AAAATCATAA AAGTACAAGA AAATCCAGCT GCATTTGTTA GAGCTATACA GCAATATGTT 
GATCCTGATG TTCAGCTGGT AATGTGCATT CTGCCTTCTA ATCAGAAGAC CTATTATGAT 
TCCATTAAAA AATATTTGAG CTCAGACTGC CCAGTCCCAA GCCAATGTGT GCTTGCTCGG 
ACCTTGAATA AACAGGGCAT GATGATGAGT ATCGCCACCA AGATCGCTAT GCAGATGACT 
TGCAAGCTCG GAGGCGAGCT GTGGGCTGTG GAAATACCTT TAAAGTCCCT GATGGTGGTC 
GGTATTGATG TCTGTAAAGA TGCACTCAGC AAGGACGTGA TGGTTGTTGG ATGCGTGGCC 
AGTGTTAACC CCAGAATCAC CAGGTGGTTT TCCCGCTGTA TCCTTCAGAG AACAATGACT 
GATGTTGCAG ATTGCTTGAA AGTTTTCATG ACTGGAGCAC TCAACAAATG GTACAAGTAC 
AATCATGATT TGCCAGCACG GATAATTGTG TACCGTGCTG GTGTAGGGGA TGGTCAGCTG 
AAAACACTTA TTGAATATGA AGTCCCACAG CTGCTGAGCA GTGTGGCAGA ATCCAGCTCA 
AATACCAGCT CAAGACTGTC GGTGATTGTG GTCAGGAAGA AGTGCATGCC ACGATTCTTT 
ACCGAAATGA ACCGCACTGT ACAGAACCCC CCACTTGGCA CTGTTGTGGA TTCAGAAGCA 
ACACGTAACG AATGGTATGA CTTTTATCTG ATCAGCCAGG TGGCCTGCCG GGGAACTGTT 
AGTCCTACCT ACTATAATGT CATCTATGAT GACAACGGCT TGAAGCCCGA CCATATGCAG 
AGACTTACAT TCAAATTGTG CCACCTGTAC TACAACTGGC CGGGCATAGT CAGTGTCCCA 
GCACCATGTC AGTATGCTCA CAAGCTGACC TTTCTGGTGG CACAAAGCAT TCATAAAGAA 
CCCAGTCTGG AATTAGCCAA CCATCTCTTC TACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of Piwi protein MIWI2 defines a distinct population of multiciliated cells.
The Journal of clinical investigation127(10)3866-3876(2017 Oct)
Wasserman GA,Szymaniak AD,Hinds AC,Yamamoto K,Kamata H,Smith NM,Hilliard KL,Carrieri C,Labadorf AT,Quinton LJ,Ai X,Varelas X,Chen F,Mizgerd JP,Fine A,O'Carroll D,Jones MR

Possible role of HIWI2 in modulating tight junction proteins in retinal pigment epithelial cells through Akt signaling pathway.
Molecular and cellular biochemistry427(1-2)145-156(2017 Mar)
Sivagurunathan S,Palanisamy K,Arunachalam JP,Chidambaram S

PIWI-like protein, HIWI2 is aberrantly expressed in retinoblastoma cells and affects cell-cycle potentially through OTX2.
Cellular & molecular biology letters2217(2017)
Sivagurunathan S,Arunachalam JP,Chidambaram S

Co-expression of Piwil2/Piwil4 in nucleus indicates poor prognosis of hepatocellular carcinoma.
Oncotarget8(3)4607-4617(2017 Jan)
Zeng G,Zhang D,Liu X,Kang Q,Fu Y,Tang B,Guo W,Zhang Y,Wei G,He D