NEK7 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEK7
Entrez Gene ID	140609
Full Name	NIMA related kinase 7
General protein information	Preferred Names NIMA related kinase 7 Names serine/threonine-protein kinase Nek7 NIMA (never in mitosis gene a)-related kinase 7 never in mitosis A-related kinase 7 nimA-related protein kinase 7
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q31.3
Summary	NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002].
Disorder MIM:	606848

mRNA and Protein(s)

mRNA	Protein	Name
NM_133494.2	NP_598001.1	serine/threonine-protein kinase Nek7
XM_017000344.1	XP_016855833.1	serine/threonine-protein kinase Nek7 isoform X1
XM_017000346.1	XP_016855835.1	serine/threonine-protein kinase Nek7 isoform X2
XM_017000347.1	XP_016855836.1	serine/threonine-protein kinase Nek7 isoform X3
XM_017000348.1	XP_016855837.1	serine/threonine-protein kinase Nek7 isoform X3
NM_133494.3	NP_598001.1	serine/threonine-protein kinase Nek7
XM_011509209.1	XP_011507511.1	serine/threonine-protein kinase Nek7 isoform X4
XM_017000345.1	XP_016855834.1	serine/threonine-protein kinase Nek7 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NEK7	XP_003587160.1
Rattus norvegicus (Norway rat)	Nek7	NP_001101816.1
Caenorhabditis elegans (roundworm)	nekl-3	NP_510080.2
Xenopus tropicalis (tropical clawed frog)	nek7	NP_001072574.1
Canis lupus familiaris (dog)	NEK7	XP_857029.1
Gallus gallus (chicken)	NEK7	NP_001026435.1
Danio rerio (zebrafish)	nek7	NP_001003617.1
Homo sapiens (human)	NEK7	NP_598001.1
Pan troglodytes (chimpanzee)	NEK7	XP_003949711.1
	NEK7	XP_002802037.1
Mus musculus (house mouse)	Nek7	NP_067618.1

Gene Ontology
Bibliography

Related articles in PubMed

A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase.
Cullati SN, Kabeche L, Kettenbach AN, Gerber SA
The Journal of cell biology216(8)2339-2354(2017 Aug)

NEK7 is required for G1 progression and procentriole formation.
Gupta A, Tsuchiya Y, Ohta M, Shiratsuchi G, Kitagawa D
Molecular biology of the cell28(15)2123-2134(2017 Jul)

Nek7 Protects Telomeres from Oxidative DNA Damage by Phosphorylation and Stabilization of TRF1.
Tan R, Nakajima S, Wang Q, Sun H, Xue J, Wu J, Hellwig S, Zeng X, Yates NA, Smithgall TE, Lei M, Jiang Y, Levine AS, Su B, Lan L
Molecular cell65(5)818-831.e5(2017 Mar)

NEK7: a novel promising therapy target for NLRP3-related inflammatory diseases.
Xu J, Lu L, Li L
Acta biochimica et biophysica Sinica48(10)966-968(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Signaling cascade of the NIMA-related kinases (Neks) Nek6, Nek7, and Nek9 is required for the localization and function of two kinesins essential for cytokinesis, Mklp2 and Kif14 to properly coordinate cytokinesis.
Title: A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase.

Results demonstrate that NEK7 is involved in the timely regulation of G1 progression, S-phase entry, and procentriole formation.
Title: NEK7 is required for G1 progression and procentriole formation.

TRF1 phosphorylation by Nek7 favors the binding of shelterin protein TIN2 and disfavors E3 ligase Fbx4 interaction, thus preventing TRF1 ubiquitination and proteasome degradation to maintain telomere integrity.
Title: Nek7 Protects Telomeres from Oxidative DNA Damage by Phosphorylation and Stabilization of TRF1.

Studies indicate that NIMA related kinase 7 (NEK7) targets at NLRP3 protein and involves in the activation of NLRP3 inflammasome.
Title: NEK7: a novel promising therapy target for NLRP3-related inflammatory diseases.

Study highlights for the first time the possible role of Nek7 in HCC progression. Nek7 would be a useful biomarker that early predicts HCC patients at higher risk of poor prognosis.
Title: Nek7 is overexpressed in hepatocellular carcinoma and promotes hepatocellular carcinoma cell proliferation in vitro and in vivo.

The following NEK7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32010	NM_133494.3 Latest version!	Homo sapiens NIMA related kinase 7 (NEK7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu75897	XM_011509209.1 Latest version!	Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu83155	XM_017000345.1 Latest version!	Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu83155	XM_017000344.1 Latest version!	Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu83156	XM_017000346.1 Latest version!	Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu83157	XM_017000348.1 Latest version!	Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu83157	XM_017000347.1 Latest version!	Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu32010	NM_133494.2	Homo sapiens NIMA related kinase 7 (NEK7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEK7 ( NM_133494.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_598001.1 Homo sapiens NIMA related kinase 7 (NEK7), mRNA.

Price & Availability↑

CloneID	OHu32010
Clone ID Related Accession (Same CDS sequence)	NM_133494.2 , NM_133494.3
Accession Version	NM_133494.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-19
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek7
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB498485.1, AK313411.1 and AC093419.2. On Dec 5, 2008 this sequence version replaced NM_133494.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.241144.1, SRR1163658.503772.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGCATTTTAA GAAGCAAAAG AGGCTAATTC CTGAAAGAAC TGTTTGGAAG 
TATTTTGTTC AGCTTTGCAG TGCATTGGAA CACATGCATT CTCGAAGAGT CATGCATAGA 
GATATAAAAC CAGCTAATGT GTTCATTACA GCCACTGGGG TGGTAAAACT TGGAGATCTT 
GGGCTTGGCC GGTTTTTCAG CTCAAAAACC ACAGCTGCAC ATTCTTTAGT TGGTACGCCT 
TATTACATGT CTCCAGAGAG AATACATGAA AATGGATACA ACTTCAAATC TGACATCTGG 
TCTCTTGGCT GTCTACTATA TGAGATGGCT GCATTACAAA GTCCTTTCTA TGGTGACAAA 
ATGAATTTAT ACTCACTGTG TAAGAAGATA GAACAGTGTG ACTACCCACC TCTTCCTTCA 
GATCACTATT CAGAAGAACT CCGACAGTTA GTTAATATGT GCATCAACCC AGATCCAGAG 
AAGCGACCAG ACGTCACCTA TGTTTATGAC GTAGCAAAGA GGATGCATGC ATGCACTGCA 
AGCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598001.1
CDS	328..1236
Translation	MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTASS

Target ORF information:

RefSeq Version	NM_133494.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 7 (NEK7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133494.2

ORF Insert Sequence:

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGCATTTTAA GAAGCAAAAG AGGCTAATTC CTGAAAGAAC TGTTTGGAAG 
TATTTTGTTC AGCTTTGCAG TGCATTGGAA CACATGCATT CTCGAAGAGT CATGCATAGA 
GATATAAAAC CAGCTAATGT GTTCATTACA GCCACTGGGG TGGTAAAACT TGGAGATCTT 
GGGCTTGGCC GGTTTTTCAG CTCAAAAACC ACAGCTGCAC ATTCTTTAGT TGGTACGCCT 
TATTACATGT CTCCAGAGAG AATACATGAA AATGGATACA ACTTCAAATC TGACATCTGG 
TCTCTTGGCT GTCTACTATA TGAGATGGCT GCATTACAAA GTCCTTTCTA TGGTGACAAA 
ATGAATTTAT ACTCACTGTG TAAGAAGATA GAACAGTGTG ACTACCCACC TCTTCCTTCA 
GATCACTATT CAGAAGAACT CCGACAGTTA GTTAATATGT GCATCAACCC AGATCCAGAG 
AAGCGACCAG ACGTCACCTA TGTTTATGAC GTAGCAAAGA GGATGCATGC ATGCACTGCA 
AGCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK7 ( NM_133494.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_598001.1 Homo sapiens NIMA related kinase 7 (NEK7), mRNA.

Price & Availability↑

CloneID	OHu32010
Clone ID Related Accession (Same CDS sequence)	NM_133494.2 , NM_133494.3
Accession Version	NM_133494.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek7
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB498485.1, AK313411.1 and AC093419.2. On Nov 23, 2018 this sequence version replaced NM_133494.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.241144.1, SRR1163658.503772.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000367385.9/ ENSP00000356355.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGCATTTTAA GAAGCAAAAG AGGCTAATTC CTGAAAGAAC TGTTTGGAAG 
TATTTTGTTC AGCTTTGCAG TGCATTGGAA CACATGCATT CTCGAAGAGT CATGCATAGA 
GATATAAAAC CAGCTAATGT GTTCATTACA GCCACTGGGG TGGTAAAACT TGGAGATCTT 
GGGCTTGGCC GGTTTTTCAG CTCAAAAACC ACAGCTGCAC ATTCTTTAGT TGGTACGCCT 
TATTACATGT CTCCAGAGAG AATACATGAA AATGGATACA ACTTCAAATC TGACATCTGG 
TCTCTTGGCT GTCTACTATA TGAGATGGCT GCATTACAAA GTCCTTTCTA TGGTGACAAA 
ATGAATTTAT ACTCACTGTG TAAGAAGATA GAACAGTGTG ACTACCCACC TCTTCCTTCA 
GATCACTATT CAGAAGAACT CCGACAGTTA GTTAATATGT GCATCAACCC AGATCCAGAG 
AAGCGACCAG ACGTCACCTA TGTTTATGAC GTAGCAAAGA GGATGCATGC ATGCACTGCA 
AGCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598001.1
CDS	308..1216
Translation	MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTASS

Target ORF information:

RefSeq Version	NM_133494.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 7 (NEK7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133494.3

ORF Insert Sequence:

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGCATTTTAA GAAGCAAAAG AGGCTAATTC CTGAAAGAAC TGTTTGGAAG 
TATTTTGTTC AGCTTTGCAG TGCATTGGAA CACATGCATT CTCGAAGAGT CATGCATAGA 
GATATAAAAC CAGCTAATGT GTTCATTACA GCCACTGGGG TGGTAAAACT TGGAGATCTT 
GGGCTTGGCC GGTTTTTCAG CTCAAAAACC ACAGCTGCAC ATTCTTTAGT TGGTACGCCT 
TATTACATGT CTCCAGAGAG AATACATGAA AATGGATACA ACTTCAAATC TGACATCTGG 
TCTCTTGGCT GTCTACTATA TGAGATGGCT GCATTACAAA GTCCTTTCTA TGGTGACAAA 
ATGAATTTAT ACTCACTGTG TAAGAAGATA GAACAGTGTG ACTACCCACC TCTTCCTTCA 
GATCACTATT CAGAAGAACT CCGACAGTTA GTTAATATGT GCATCAACCC AGATCCAGAG 
AAGCGACCAG ACGTCACCTA TGTTTATGAC GTAGCAAAGA GGATGCATGC ATGCACTGCA 
AGCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK7 ( XM_011509209.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507511.1 Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu75897
Clone ID Related Accession (Same CDS sequence)	XM_011509209.1
Accession Version	XM_011509209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGCTCCGACA GTTAGTTAAT ATGTGCATCA ACCCAGATCC AGAGAAGCGA 
CCAGACGTCA CCTATGTTTA TGACGTAGCA AAGAGGATGC ATGCATGCAC TGCAAGCAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507511.1
CDS	306..788
Translation	MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKLRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTASS

Target ORF information:

RefSeq Version	XM_011509209.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509209.1

ORF Insert Sequence:

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGCTCCGACA GTTAGTTAAT ATGTGCATCA ACCCAGATCC AGAGAAGCGA 
CCAGACGTCA CCTATGTTTA TGACGTAGCA AAGAGGATGC ATGCATGCAC TGCAAGCAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK7 ( XM_017000344.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855833.1 Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu83155
Clone ID Related Accession (Same CDS sequence)	XM_017000344.1 , XM_017000345.1
Accession Version	XM_017000344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGTTCTTATT TTTAATTTTC AAAAAGCATT TTAAGAAGCA AAAGAGGCTA 
ATTCCTGAAA GAACTGTTTG GAAGTATTTT GTTCAGCTTT GCAGTGCATT GGAACACATG 
CATTCTCGAA GAGTCATGCA TAGAGATATA AAACCAGCTA ATGTGTTCAT TACAGCCACT 
GGGGTGGTAA AACTTGGAGA TCTTGGGCTT GGCCGGTTTT TCAGCTCAAA AACCACAGCT 
GCACATTCTT TAGTTGGTAC GCCTTATTAC ATGTCTCCAG AGAGAATACA TGAAAATGGA 
TACAACTTCA AATCTGACAT CTGGTCTCTT GGCTGTCTAC TATATGAGAT GGCTGCATTA 
CAAAGTCCTT TCTATGGTGA CAAAATGAAT TTATACTCAC TGTGTAAGAA GATAGAACAG 
TGTGACTACC CACCTCTTCC TTCAGATCAC TATTCAGAAG AACTCCGACA GTTAGTTAAT 
ATGTGCATCA ACCCAGATCC AGAGAAGCGA CCAGACGTCA CCTATGTTTA TGACGTAGCA 
AAGAGGATGC ATGCATGCAC TGCAAGCAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855833.1
CDS	306..1238
Translation	MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKFLFLIFKKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTASS

Target ORF information:

RefSeq Version	XM_017000344.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000344.1

ORF Insert Sequence:

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGTTCTTATT TTTAATTTTC AAAAAGCATT TTAAGAAGCA AAAGAGGCTA 
ATTCCTGAAA GAACTGTTTG GAAGTATTTT GTTCAGCTTT GCAGTGCATT GGAACACATG 
CATTCTCGAA GAGTCATGCA TAGAGATATA AAACCAGCTA ATGTGTTCAT TACAGCCACT 
GGGGTGGTAA AACTTGGAGA TCTTGGGCTT GGCCGGTTTT TCAGCTCAAA AACCACAGCT 
GCACATTCTT TAGTTGGTAC GCCTTATTAC ATGTCTCCAG AGAGAATACA TGAAAATGGA 
TACAACTTCA AATCTGACAT CTGGTCTCTT GGCTGTCTAC TATATGAGAT GGCTGCATTA 
CAAAGTCCTT TCTATGGTGA CAAAATGAAT TTATACTCAC TGTGTAAGAA GATAGAACAG 
TGTGACTACC CACCTCTTCC TTCAGATCAC TATTCAGAAG AACTCCGACA GTTAGTTAAT 
ATGTGCATCA ACCCAGATCC AGAGAAGCGA CCAGACGTCA CCTATGTTTA TGACGTAGCA 
AAGAGGATGC ATGCATGCAC TGCAAGCAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK7 ( XM_017000345.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855834.1 Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83155
Clone ID Related Accession (Same CDS sequence)	XM_017000344.1 , XM_017000345.1
Accession Version	XM_017000345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGTTCTTATT TTTAATTTTC AAAAAGCATT TTAAGAAGCA AAAGAGGCTA 
ATTCCTGAAA GAACTGTTTG GAAGTATTTT GTTCAGCTTT GCAGTGCATT GGAACACATG 
CATTCTCGAA GAGTCATGCA TAGAGATATA AAACCAGCTA ATGTGTTCAT TACAGCCACT 
GGGGTGGTAA AACTTGGAGA TCTTGGGCTT GGCCGGTTTT TCAGCTCAAA AACCACAGCT 
GCACATTCTT TAGTTGGTAC GCCTTATTAC ATGTCTCCAG AGAGAATACA TGAAAATGGA 
TACAACTTCA AATCTGACAT CTGGTCTCTT GGCTGTCTAC TATATGAGAT GGCTGCATTA 
CAAAGTCCTT TCTATGGTGA CAAAATGAAT TTATACTCAC TGTGTAAGAA GATAGAACAG 
TGTGACTACC CACCTCTTCC TTCAGATCAC TATTCAGAAG AACTCCGACA GTTAGTTAAT 
ATGTGCATCA ACCCAGATCC AGAGAAGCGA CCAGACGTCA CCTATGTTTA TGACGTAGCA 
AAGAGGATGC ATGCATGCAC TGCAAGCAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855834.1
CDS	413..1345
Translation	MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKFLFLIFKKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTASS

Target ORF information:

RefSeq Version	XM_017000345.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000345.1

ORF Insert Sequence:

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGTTCTTATT TTTAATTTTC AAAAAGCATT TTAAGAAGCA AAAGAGGCTA 
ATTCCTGAAA GAACTGTTTG GAAGTATTTT GTTCAGCTTT GCAGTGCATT GGAACACATG 
CATTCTCGAA GAGTCATGCA TAGAGATATA AAACCAGCTA ATGTGTTCAT TACAGCCACT 
GGGGTGGTAA AACTTGGAGA TCTTGGGCTT GGCCGGTTTT TCAGCTCAAA AACCACAGCT 
GCACATTCTT TAGTTGGTAC GCCTTATTAC ATGTCTCCAG AGAGAATACA TGAAAATGGA 
TACAACTTCA AATCTGACAT CTGGTCTCTT GGCTGTCTAC TATATGAGAT GGCTGCATTA 
CAAAGTCCTT TCTATGGTGA CAAAATGAAT TTATACTCAC TGTGTAAGAA GATAGAACAG 
TGTGACTACC CACCTCTTCC TTCAGATCAC TATTCAGAAG AACTCCGACA GTTAGTTAAT 
ATGTGCATCA ACCCAGATCC AGAGAAGCGA CCAGACGTCA CCTATGTTTA TGACGTAGCA 
AAGAGGATGC ATGCATGCAC TGCAAGCAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK7 ( XM_017000346.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855835.1 Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu83156
Clone ID Related Accession (Same CDS sequence)	XM_017000346.1
Accession Version	XM_017000346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGTTCTTATT TTTAATTTTC AAAAAGCATT TTAAGAAGCA AAAGAGGCTA 
ATTCCTGAAA GAACTGTTTG GAAGTATTTT GTTCAGCTTT GCAGTGCATT GGAACACATG 
CATTCTCGAA GAGTCATGCA TAGAGATATA AAACCAGCTA ATGTGTTCAT TACAGCCACT 
GGGGTGGTAA AACTTGGAGA TCTTGGGCTT GGCCGGTTTT TCAGCTCAAA AACCACAGCT 
GCACATTCTT TAGTTGGTAC GCCTTATTAC ATGTCTCCAG AGAGAATACA TGAAAATGGA 
TACAACTTCA AATCTGACAT CTGGTCTCTT GGCTGTCTAC TATATGAGCT CCGACAGTTA 
GTTAATATGT GCATCAACCC AGATCCAGAG AAGCGACCAG ACGTCACCTA TGTTTATGAC 
GTAGCAAAGA GGATGCATGC ATGCACTGCA AGCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855835.1
CDS	306..1124
Translation	MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKFLFLIFKKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTASS

Target ORF information:

RefSeq Version	XM_017000346.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000346.1

ORF Insert Sequence:

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGTTCTTATT TTTAATTTTC AAAAAGCATT TTAAGAAGCA AAAGAGGCTA 
ATTCCTGAAA GAACTGTTTG GAAGTATTTT GTTCAGCTTT GCAGTGCATT GGAACACATG 
CATTCTCGAA GAGTCATGCA TAGAGATATA AAACCAGCTA ATGTGTTCAT TACAGCCACT 
GGGGTGGTAA AACTTGGAGA TCTTGGGCTT GGCCGGTTTT TCAGCTCAAA AACCACAGCT 
GCACATTCTT TAGTTGGTAC GCCTTATTAC ATGTCTCCAG AGAGAATACA TGAAAATGGA 
TACAACTTCA AATCTGACAT CTGGTCTCTT GGCTGTCTAC TATATGAGCT CCGACAGTTA 
GTTAATATGT GCATCAACCC AGATCCAGAG AAGCGACCAG ACGTCACCTA TGTTTATGAC 
GTAGCAAAGA GGATGCATGC ATGCACTGCA AGCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK7 ( XM_017000347.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855836.1 Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu83157
Clone ID Related Accession (Same CDS sequence)	XM_017000347.1 , XM_017000348.1
Accession Version	XM_017000347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGTTCTTATT TTTAATTTTC AAAAAGCATT TTAAGAAGCA AAAGAGGCTA 
ATTCCTGAAA GAACTGTTTG GAAGTATTTT GTTCAGCTTT GCAGTGCATT GGAACACATG 
CATTCTCGAA GAGTCATGCA TAGAGATATA AAACCAGCTA ATGTGTTCAT TACAGCCACT 
GGGGTGGTAA AACTTGGAGA TCTTGGGCTT GGCCGGTTTT TCAGCTCAAA AACCACAGCT 
GCACATTCTT TAGCTGTTAA AAGAAGTCAC AACATTCTTG AGGCAACAGT AATCGTAGAC 
ACAGCTGTCA TGAATCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855836.1
CDS	306..986
Translation	MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKFLFLIFKKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLAVKRSHNILEATVIVDTAVMNL

Target ORF information:

RefSeq Version	XM_017000347.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000347.1

ORF Insert Sequence:

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGTTCTTATT TTTAATTTTC AAAAAGCATT TTAAGAAGCA AAAGAGGCTA 
ATTCCTGAAA GAACTGTTTG GAAGTATTTT GTTCAGCTTT GCAGTGCATT GGAACACATG 
CATTCTCGAA GAGTCATGCA TAGAGATATA AAACCAGCTA ATGTGTTCAT TACAGCCACT 
GGGGTGGTAA AACTTGGAGA TCTTGGGCTT GGCCGGTTTT TCAGCTCAAA AACCACAGCT 
GCACATTCTT TAGCTGTTAA AAGAAGTCAC AACATTCTTG AGGCAACAGT AATCGTAGAC 
ACAGCTGTCA TGAATCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK7 ( XM_017000348.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855837.1 Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu83157
Clone ID Related Accession (Same CDS sequence)	XM_017000347.1 , XM_017000348.1
Accession Version	XM_017000348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGTTCTTATT TTTAATTTTC AAAAAGCATT TTAAGAAGCA AAAGAGGCTA 
ATTCCTGAAA GAACTGTTTG GAAGTATTTT GTTCAGCTTT GCAGTGCATT GGAACACATG 
CATTCTCGAA GAGTCATGCA TAGAGATATA AAACCAGCTA ATGTGTTCAT TACAGCCACT 
GGGGTGGTAA AACTTGGAGA TCTTGGGCTT GGCCGGTTTT TCAGCTCAAA AACCACAGCT 
GCACATTCTT TAGCTGTTAA AAGAAGTCAC AACATTCTTG AGGCAACAGT AATCGTAGAC 
ACAGCTGTCA TGAATCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855837.1
CDS	306..986
Translation	MDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKFLFLIFKKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLAVKRSHNILEATVIVDTAVMNL

Target ORF information:

RefSeq Version	XM_017000348.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 7 (NEK7), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000348.1

ORF Insert Sequence:

ATGGATGAGC AATCACAAGG AATGCAAGGG CCACCTGTTC CTCAGTTCCA ACCACAGAAG 
GCCTTACGAC CGGATATGGG CTATAATACA TTAGCCAACT TTCGAATAGA AAAGAAAATT 
GGTCGCGGAC AATTTAGTGA AGTTTATAGA GCAGCCTGTC TCTTGGATGG AGTACCAGTA 
GCTTTAAAAA AAGTGCAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGCATC 
AAAGAAATAG ATCTTCTTAA GCAACTCAAC CATCCAAATG TAATAAAATA TTATGCATCA 
TTCATTGAAG ATAATGAACT AAACATAGTT TTGGAACTAG CAGATGCTGG CGACCTATCC 
AGAATGATCA AGTTCTTATT TTTAATTTTC AAAAAGCATT TTAAGAAGCA AAAGAGGCTA 
ATTCCTGAAA GAACTGTTTG GAAGTATTTT GTTCAGCTTT GCAGTGCATT GGAACACATG 
CATTCTCGAA GAGTCATGCA TAGAGATATA AAACCAGCTA ATGTGTTCAT TACAGCCACT 
GGGGTGGTAA AACTTGGAGA TCTTGGGCTT GGCCGGTTTT TCAGCTCAAA AACCACAGCT 
GCACATTCTT TAGCTGTTAA AAGAAGTCAC AACATTCTTG AGGCAACAGT AATCGTAGAC 
ACAGCTGTCA TGAATCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase.
The Journal of cell biology216(8)2339-2354(2017 Aug)
Cullati SN,Kabeche L,Kettenbach AN,Gerber SA

NEK7 is required for G1 progression and procentriole formation.
Molecular biology of the cell28(15)2123-2134(2017 Jul)
Gupta A,Tsuchiya Y,Ohta M,Shiratsuchi G,Kitagawa D

Nek7 Protects Telomeres from Oxidative DNA Damage by Phosphorylation and Stabilization of TRF1.
Molecular cell65(5)818-831.e5(2017 Mar)
Tan R,Nakajima S,Wang Q,Sun H,Xue J,Wu J,Hellwig S,Zeng X,Yates NA,Smithgall TE,Lei M,Jiang Y,Levine AS,Su B,Lan L

NEK7: a novel promising therapy target for NLRP3-related inflammatory diseases.
Acta biochimica et biophysica Sinica48(10)966-968(2016 Oct)
Xu J,Lu L,Li L

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NEK7 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

オンラインフォーム