RMI2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RMI2
Entrez Gene ID	116028
Full Name	RecQ mediated genome instability 2
Synonyms	BLAP18,C16orf75
General protein information	Preferred Names RecQ mediated genome instability 2 Names recQ-mediated genome instability protein 2 BLM-associated protein of 18 kDa RMI2, RecQ mediated genome instability 2, homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.13
Summary	RMI2 is a component of the BLM (RECQL3; MIM 604610) complex, which plays a role in homologous recombination-dependent DNA repair and is essential for genome stability (Xu et al., 2008 [PubMed 18923082]).[supplied by OMIM, Nov 2008].
Disorder MIM:	612426

mRNA and Protein(s)

mRNA	Protein	Name
NM_152308.3	NP_689521.1	recQ-mediated genome instability protein 2
NM_152308.2	NP_689521.1	recQ-mediated genome instability protein 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RMI2	XP_852348.2
Gallus gallus (chicken)	RMI2	NP_001006174.1
Xenopus tropicalis (tropical clawed frog)	rmi2	XP_004918006.1
Rattus norvegicus (Norway rat)	Rmi2	XP_573037.1
Homo sapiens (human)	RMI2	NP_689521.1
	C16orf75	XP_001104895.1
Bos taurus (cattle)	RMI2	NP_001093190.1
Danio rerio (zebrafish)	LOC563218	XP_691674.2
Mus musculus (house mouse)	Rmi2	NP_001156404.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Loss of RMI2 Increases Genome Instability and Causes a Bloom-Like Syndrome.
Hudson DF, Amor DJ, Boys A, Butler K, Williams L, Zhang T, Kalitsis P
PLoS genetics12(12)e1006483(2016 Dec)

Multifaceted role of the Topo III?-RMI1-RMI2 complex and DNA2 in the BLM-dependent pathway of DNA break end resection.
Daley JM, Chiba T, Xue X, Niu H, Sung P
Nucleic acids research42(17)11083-91(2014)

Monopolar spindle 1 (MPS1) protein-dependent phosphorylation of RecQ-mediated genome instability protein 2 (RMI2) at serine 112 is essential for BLM-Topo III ?-RMI1-RMI2 (BTR) protein complex function upon spindle assembly checkpoint (SAC) activation during mitosis.
Pradhan A, Singh TR, Ali AM, Wahengbam K, Meetei AR
The Journal of biological chemistry288(47)33500-8(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In both patient and knockout cell lines reduced localisation of BLM to ultra fine DNA bridges and FANCD2 at foci linking bridges are observed. Overall, loss of RMI2 produces a partially active BLM complex with mild features of Bloom syndrome.
Title: Loss of RMI2 Increases Genome Instability and Causes a Bloom-Like Syndrome.

The results show that Topo IIIalpha stimulates DNA unwinding by BLM in a manner that is potentiated by RMI1-RMI2, and that the processivity of resection is reliant on the Topo IIIalpha-RMI1-RMI2 complex.
Title: Multifaceted role of the Topo III?-RMI1-RMI2 complex and DNA2 in the BLM-dependent pathway of DNA break end resection.

two proteins that interact with BLM, RMI1 and RMI2, are phosphorylated upon SAC activation, and, like BLM, RMI1, and RMI2, are phosphorylated in an MPS1-dependent manner.
Title: Monopolar spindle 1 (MPS1) protein-dependent phosphorylation of RecQ-mediated genome instability protein 2 (RMI2) at serine 112 is essential for BLM-Topo III ?-RMI1-RMI2 (BTR) protein complex function upon spindle assembly checkpoint (SAC) activation during mitosis.

Observational study and meta-analysis of gene-disease association. (HuGE Navigator)
Title: Analysis of SNPs with an effect on gene expression identifies UBE2L3 and BCL3 as potential new risk genes for Crohn's disease.

Crystal structures of RMI1 and RMI2, two OB-fold regulatory subunits of the BLM complex
Title: Crystal structures of RMI1 and RMI2, two OB-fold regulatory subunits of the BLM complex.

The following RMI2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RMI2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55920	NM_152308.3 Latest version!	Homo sapiens RecQ mediated genome instability 2 (RMI2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu55920	NM_152308.2	Homo sapiens RecQ mediated genome instability 2 (RMI2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RMI2 ( NM_152308.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689521.1 Homo sapiens RecQ mediated genome instability 2 (RMI2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55920
Clone ID Related Accession (Same CDS sequence)	NM_152308.3 , NM_152308.2
Accession Version	NM_152308.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	recQ-mediated genome instability protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC022427.1, BC039361.1 and AK123764.1. On Nov 22, 2018 this sequence version replaced NM_152308.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.135542.1, AK123764.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000312499.6/ ENSP00000310356.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGG CTGCGGACTC GTTCTCAGGC GGCCCCGCGG GGGTGCGGCT TCCGAGGTCG 
CCGCCACTCA AGGTGCTGGC GGAGCAGCTG CGGCGCGACG CGGAGGGCGG CCCGGGCGCG 
TGGCGGCTGT CACGGGCGGC GGCGGGCCGC GGGCCGCTGG ACCTGGCGGC CGTGTGGATG 
CAGGGCAGGG TAGTGATGGC GGACCGCGGC GAGGCTCGGC TGAGGGACCC GAGCGGGGAC 
TTCTCGGTCC GCGGCCTGGA GCGGGTGCCG CGCGGGCGGC CCTGTCTAGT CCCAGGAAAG 
TATGTGATGG TGATGGGAGT GGTTCAGGCC TGCAGCCCTG AGCCCTGCCT GCAGGCTGTG 
AAGATGACAG ACCTTTCTGA TAATCCCATC CATGAAAGTA TGTGGGAACT GGAGGTAGAA 
GATTTACACA GGAATATTCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689521.1
CDS	14..457
Translation	MAAAADSFSGGPAGVRLPRSPPLKVLAEQLRRDAEGGPGAWRLSRAAAGRGPLDLAAVWMQGRVVMADRGEARLRDPSGDFSVRGLERVPRGRPCLVPGKYVMVMGVVQACSPEPCLQAVKMTDLSDNPIHESMWELEVEDLHRNIP

Target ORF information:

RefSeq Version	NM_152308.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RecQ mediated genome instability 2 (RMI2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152308.3

ORF Insert Sequence:

ATGGCGGCGG CTGCGGACTC GTTCTCAGGC GGCCCCGCGG GGGTGCGGCT TCCGAGGTCG 
CCGCCACTCA AGGTGCTGGC GGAGCAGCTG CGGCGCGACG CGGAGGGCGG CCCGGGCGCG 
TGGCGGCTGT CACGGGCGGC GGCGGGCCGC GGGCCGCTGG ACCTGGCGGC CGTGTGGATG 
CAGGGCAGGG TAGTGATGGC GGACCGCGGC GAGGCTCGGC TGAGGGACCC GAGCGGGGAC 
TTCTCGGTCC GCGGCCTGGA GCGGGTGCCG CGCGGGCGGC CCTGTCTAGT CCCAGGAAAG 
TATGTGATGG TGATGGGAGT GGTTCAGGCC TGCAGCCCTG AGCCCTGCCT GCAGGCTGTG 
AAGATGACAG ACCTTTCTGA TAATCCCATC CATGAAAGTA TGTGGGAACT GGAGGTAGAA 
GATTTACACA GGAATATTCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RMI2 ( NM_152308.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_689521.1 Homo sapiens RecQ mediated genome instability 2 (RMI2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55920
Clone ID Related Accession (Same CDS sequence)	NM_152308.3 , NM_152308.2
Accession Version	NM_152308.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	recQ-mediated genome instability protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC009121.8, BC022427.1, BC039361.1 and AK123764.1. On Jan 29, 2015 this sequence version replaced NM_152308.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. ##Evidence-Data-START## Transcript exon combination :: AK123764.1, SRR1163655.626438.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CTGCGGACTC GTTCTCAGGC GGCCCCGCGG GGGTGCGGCT TCCGAGGTCG 
CCGCCACTCA AGGTGCTGGC GGAGCAGCTG CGGCGCGACG CGGAGGGCGG CCCGGGCGCG 
TGGCGGCTGT CACGGGCGGC GGCGGGCCGC GGGCCGCTGG ACCTGGCGGC CGTGTGGATG 
CAGGGCAGGG TAGTGATGGC GGACCGCGGC GAGGCTCGGC TGAGGGACCC GAGCGGGGAC 
TTCTCGGTCC GCGGCCTGGA GCGGGTGCCG CGCGGGCGGC CCTGTCTAGT CCCAGGAAAG 
TATGTGATGG TGATGGGAGT GGTTCAGGCC TGCAGCCCTG AGCCCTGCCT GCAGGCTGTG 
AAGATGACAG ACCTTTCTGA TAATCCCATC CATGAAAGTA TGTGGGAACT GGAGGTAGAA 
GATTTACACA GGAATATTCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689521.1
CDS	35..478
Translation	MAAAADSFSGGPAGVRLPRSPPLKVLAEQLRRDAEGGPGAWRLSRAAAGRGPLDLAAVWMQGRVVMADRGEARLRDPSGDFSVRGLERVPRGRPCLVPGKYVMVMGVVQACSPEPCLQAVKMTDLSDNPIHESMWELEVEDLHRNIP

Target ORF information:

RefSeq Version	NM_152308.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RecQ mediated genome instability 2 (RMI2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152308.2

ORF Insert Sequence:

ATGGCGGCGG CTGCGGACTC GTTCTCAGGC GGCCCCGCGG GGGTGCGGCT TCCGAGGTCG 
CCGCCACTCA AGGTGCTGGC GGAGCAGCTG CGGCGCGACG CGGAGGGCGG CCCGGGCGCG 
TGGCGGCTGT CACGGGCGGC GGCGGGCCGC GGGCCGCTGG ACCTGGCGGC CGTGTGGATG 
CAGGGCAGGG TAGTGATGGC GGACCGCGGC GAGGCTCGGC TGAGGGACCC GAGCGGGGAC 
TTCTCGGTCC GCGGCCTGGA GCGGGTGCCG CGCGGGCGGC CCTGTCTAGT CCCAGGAAAG 
TATGTGATGG TGATGGGAGT GGTTCAGGCC TGCAGCCCTG AGCCCTGCCT GCAGGCTGTG 
AAGATGACAG ACCTTTCTGA TAATCCCATC CATGAAAGTA TGTGGGAACT GGAGGTAGAA 
GATTTACACA GGAATATTCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Loss of RMI2 Increases Genome Instability and Causes a Bloom-Like Syndrome.
PLoS genetics12(12)e1006483(2016 Dec)
Hudson DF,Amor DJ,Boys A,Butler K,Williams L,Zhang T,Kalitsis P

Multifaceted role of the Topo III?-RMI1-RMI2 complex and DNA2 in the BLM-dependent pathway of DNA break end resection.
Nucleic acids research42(17)11083-91(2014)
Daley JM,Chiba T,Xue X,Niu H,Sung P

Monopolar spindle 1 (MPS1) protein-dependent phosphorylation of RecQ-mediated genome instability protein 2 (RMI2) at serine 112 is essential for BLM-Topo III ?-RMI1-RMI2 (BTR) protein complex function upon spindle assembly checkpoint (SAC) activation during mitosis.
The Journal of biological chemistry288(47)33500-8(2013 Nov)
Pradhan A,Singh TR,Ali AM,Wahengbam K,Meetei AR