GPR83 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPR83
Entrez Gene ID	10888
Full Name	G protein-coupled receptor 83
Synonyms	GIR,GPR72
General protein information	Preferred Names G protein-coupled receptor 83 Names probable G-protein coupled receptor 83 G-protein coupled receptor 72 glucocorticoid induced receptor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q21
Disorder MIM:	605569

mRNA and Protein(s)

mRNA	Protein	Name
NM_001330345.1	NP_001317274.1	probable G-protein coupled receptor 83 isoform 2 precursor
NM_016540.4	NP_057624.3	probable G-protein coupled receptor 83 isoform 1 precursor
NM_016540.3	NP_057624.3	probable G-protein coupled receptor 83 isoform 1 precursor
NM_001330345.2	NP_001317274.1	probable G-protein coupled receptor 83 isoform 2 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GPR83	NP_001003108.1
Homo sapiens (human)	GPR83	NP_057624.3
	GPR83	XP_001088588.1
Gallus gallus (chicken)	GPR83	NP_001243067.1
Pan troglodytes (chimpanzee)	GPR83	XP_522151.2
Mus musculus (house mouse)	Gpr83	NP_034417.1
Xenopus tropicalis (tropical clawed frog)	LOC100486806	XP_002939760.1
Rattus norvegicus (Norway rat)	Gpr83	NP_536336.1

Gene Ontology
Bibliography

Related articles in PubMed

Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.
Badgandi HB, Hwang SH, Shimada IS, Loriot E, Mukhopadhyay S
The Journal of cell biology216(3)743-760(2017 Mar)

Genomic association analysis identifies multiple loci influencing antihypertensive response to an angiotensin II receptor blocker.
Turner ST, Bailey KR, Schwartz GL, Chapman AB, Chai HS, Boerwinkle E
Hypertension (Dallas, Tex. : 1979)59(6)1204-11(2012 Jun)

Inflammation in vivo is modulated by GPR83 isoform-4 but not GPR83 isoform-1 expression in regulatory T cells.
Hansen W, Westendorf AM, Toepfer T, Mauel S, Geffers R, Gruber AD, Buer J
Genes and immunity11(4)357-61(2010 Jun)

G protein-coupled receptor 83 overexpression in naive CD4+CD25- T cells leads to the induction of Foxp3+ regulatory T cells in vivo.
Hansen W, Loser K, Westendorf AM, Bruder D, Pfoertner S, Siewert C, Huehn J, Beissert S, Buer J
Journal of immunology (Baltimore, Md. : 1950)177(1)209-15(2006 Jul)

Cloning and chromosomal mapping of the mouse and human genes encoding the orphan glucocorticoid-induced receptor (GPR83).
De Moerlooze L, Williamson J, Liners F, Perret J, Parmentier M
Cytogenetics and cell genetics90(1-2)146-50(2000)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest that the 20 additional aminoacids within GPR83 isoform-4 are involved in Treg induction during inflammatory immune responses
Title: Inflammation in vivo is modulated by GPR83 isoform-4 but not GPR83 isoform-1 expression in regulatory T cells.

GPR83 is 2- to 7-fold up-regulated in all indvidual human CD4+CD25+ T regulatory cell populations analyzed and is directly linked to Foxp3 expression.
Title: G protein-coupled receptor 83 overexpression in naive CD4+CD25- T cells leads to the induction of Foxp3+ regulatory T cells in vivo.

The following GPR83 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPR83 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01719	NM_016540.4 Latest version!	Homo sapiens G protein-coupled receptor 83 (GPR83), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$251.30 $359.00
OHu64485	NM_001330345.2 Latest version!	Homo sapiens G protein-coupled receptor 83 (GPR83), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu01719	NM_016540.3	Homo sapiens G protein-coupled receptor 83 (GPR83), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$251.30 $359.00
OHu64485	NM_001330345.1	Homo sapiens G protein-coupled receptor 83 (GPR83), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPR83 ( NM_016540.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057624.3 Homo sapiens G protein-coupled receptor 83 (GPR83), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01719
Clone ID Related Accession (Same CDS sequence)	NM_016540.4 , NM_016540.3
Accession Version	NM_016540.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	probable G-protein coupled receptor 83 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK315161.1, DA317535.1, AB040973.1, AP000765.5 and AI914177.1. On Nov 23, 2018 this sequence version replaced NM_016540.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.58106.1, SRR1660803.24818.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000243673.7/ ENSP00000243673.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTCCCTC ACCTCTTGCT GCTCTGTCTC CTCCCCTTGG TGCGAGCCAC CGAGCCCCAC 
GAGGGCCGGG CCGACGAGCA GAGCGCGGAG GCGGCCCTGG CCGTGCCCAA TGCCTCGCAC 
TTCTTCTCTT GGAACAACTA CACCTTCTCC GACTGGCAGA ACTTTGTGGG CAGGAGGCGC 
TACGGCGCTG AGTCCCAGAA CCCCACGGTG AAAGCCCTGC TCATTGTGGC TTACTCCTTC 
ATCATTGTCT TCTCACTCTT TGGCAACGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGAATGCACT CGGCCACCAG CCTCTTCATC GTCAACCTGG CAGTTGCCGA CATAATGATC 
ACGCTGCTCA ACACCCCCTT CACTTTGGTT CGCTTTGTGA ACAGCACATG GATATTTGGG 
AAGGGCATGT GCCATGTCAG CCGCTTTGCC CAGTACTGCT CACTGCACGT CTCAGCACTG 
ACACTGACAG CCATTGCGGT GGATCGCCAC CAGGTCATCA TGCACCCCTT GAAACCCCGG 
ATCTCAATCA CAAAGGGTGT CATCTACATC GCTGTCATCT GGACCATGGC TACGTTCTTT 
TCACTCCCAC ATGCTATCTG CCAGAAATTA TTTACCTTCA AATACAGTGA GGACATTGTG 
CGCTCCCTCT GCCTGCCAGA CTTCCCTGAG CCAGCTGACC TCTTCTGGAA GTACCTGGAC 
TTGGCCACCT TCATCCTGCT CTACATCCTG CCCCTCCTCA TCATCTCTGT GGCCTACGCT 
CGTGTGGCCA AGAAACTGTG GCTGTGTAAT ATGATTGGCG ATGTGACCAC AGAGCAGTAC 
TTTGCCCTGC GGCGCAAAAA GAAGAAGACC ATCAAGATGT TGATGCTGGT GGTAGTCCTC 
TTTGCCCTCT GCTGGTTCCC CCTCAACTGC TACGTCCTCC TCCTGTCCAG CAAGGTCATC 
CGCACCAACA ATGCCCTCTA CTTTGCCTTC CACTGGTTTG CCATGAGCAG CACCTGCTAT 
AACCCCTTCA TATACTGCTG GCTGAACGAG AACTTCAGGA TTGAGCTAAA GGCATTACTG 
AGCATGTGTC AAAGACCTCC CAAGCCTCAG GAGGACAGGC CACCCTCCCC AGTTCCTTCC 
TTCAGGGTGG CCTGGACAGA GAAGAATGAT GGCCAGAGGG CTCCCCTTGC CAATAACCTC 
CTGCCCACCT CCCAACTCCA GTCTGGGAAG ACAGACCTGT CATCTGTGGA ACCCATTGTG 
ACGATGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057624.3
CDS	173..1444
Translation	MVPHLLLLCLLPLVRATEPHEGRADEQSAEAALAVPNASHFFSWNNYTFSDWQNFVGRRRYGAESQNPTVKALLIVAYSFIIVFSLFGNVLVCHVIFKNQRMHSATSLFIVNLAVADIMITLLNTPFTLVRFVNSTWIFGKGMCHVSRFAQYCSLHVSALTLTAIAVDRHQVIMHPLKPRISITKGVIYIAVIWTMATFFSLPHAICQKLFTFKYSEDIVRSLCLPDFPEPADLFWKYLDLATFILLYILPLLIISVAYARVAKKLWLCNMIGDVTTEQYFALRRKKKKTIKMLMLVVVLFALCWFPLNCYVLLLSSKVIRTNNALYFAFHWFAMSSTCYNPFIYCWLNENFRIELKALLSMCQRPPKPQEDRPPSPVPSFRVAWTEKNDGQRAPLANNLLPTSQLQSGKTDLSSVEPIVTMS

Target ORF information:

RefSeq Version	NM_016540.4
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 83 (GPR83), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016540.4

ORF Insert Sequence:

ATGGTCCCTC ACCTCTTGCT GCTCTGTCTC CTCCCCTTGG TGCGAGCCAC CGAGCCCCAC 
GAGGGCCGGG CCGACGAGCA GAGCGCGGAG GCGGCCCTGG CCGTGCCCAA TGCCTCGCAC 
TTCTTCTCTT GGAACAACTA CACCTTCTCC GACTGGCAGA ACTTTGTGGG CAGGAGGCGC 
TACGGCGCTG AGTCCCAGAA CCCCACGGTG AAAGCCCTGC TCATTGTGGC TTACTCCTTC 
ATCATTGTCT TCTCACTCTT TGGCAACGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGAATGCACT CGGCCACCAG CCTCTTCATC GTCAACCTGG CAGTTGCCGA CATAATGATC 
ACGCTGCTCA ACACCCCCTT CACTTTGGTT CGCTTTGTGA ACAGCACATG GATATTTGGG 
AAGGGCATGT GCCATGTCAG CCGCTTTGCC CAGTACTGCT CACTGCACGT CTCAGCACTG 
ACACTGACAG CCATTGCGGT GGATCGCCAC CAGGTCATCA TGCACCCCTT GAAACCCCGG 
ATCTCAATCA CAAAGGGTGT CATCTACATC GCTGTCATCT GGACCATGGC TACGTTCTTT 
TCACTCCCAC ATGCTATCTG CCAGAAATTA TTTACCTTCA AATACAGTGA GGACATTGTG 
CGCTCCCTCT GCCTGCCAGA CTTCCCTGAG CCAGCTGACC TCTTCTGGAA GTACCTGGAC 
TTGGCCACCT TCATCCTGCT CTACATCCTG CCCCTCCTCA TCATCTCTGT GGCCTACGCT 
CGTGTGGCCA AGAAACTGTG GCTGTGTAAT ATGATTGGCG ATGTGACCAC AGAGCAGTAC 
TTTGCCCTGC GGCGCAAAAA GAAGAAGACC ATCAAGATGT TGATGCTGGT GGTAGTCCTC 
TTTGCCCTCT GCTGGTTCCC CCTCAACTGC TACGTCCTCC TCCTGTCCAG CAAGGTCATC 
CGCACCAACA ATGCCCTCTA CTTTGCCTTC CACTGGTTTG CCATGAGCAG CACCTGCTAT 
AACCCCTTCA TATACTGCTG GCTGAACGAG AACTTCAGGA TTGAGCTAAA GGCATTACTG 
AGCATGTGTC AAAGACCTCC CAAGCCTCAG GAGGACAGGC CACCCTCCCC AGTTCCTTCC 
TTCAGGGTGG CCTGGACAGA GAAGAATGAT GGCCAGAGGG CTCCCCTTGC CAATAACCTC 
CTGCCCACCT CCCAACTCCA GTCTGGGAAG ACAGACCTGT CATCTGTGGA ACCCATTGTG 
ACGATGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR83 ( NM_016540.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057624.3 Homo sapiens G protein-coupled receptor 83 (GPR83), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01719
Clone ID Related Accession (Same CDS sequence)	NM_016540.4 , NM_016540.3
Accession Version	NM_016540.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	probable G-protein coupled receptor 83 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK315161.1, DA317535.1, AB040973.1, AP000765.5, AI914177.1 and BE670361.1. On Feb 14, 2009 this sequence version replaced NM_016540.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.58106.1, SRR1660803.24818.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCCTC ACCTCTTGCT GCTCTGTCTC CTCCCCTTGG TGCGAGCCAC CGAGCCCCAC 
GAGGGCCGGG CCGACGAGCA GAGCGCGGAG GCGGCCCTGG CCGTGCCCAA TGCCTCGCAC 
TTCTTCTCTT GGAACAACTA CACCTTCTCC GACTGGCAGA ACTTTGTGGG CAGGAGGCGC 
TACGGCGCTG AGTCCCAGAA CCCCACGGTG AAAGCCCTGC TCATTGTGGC TTACTCCTTC 
ATCATTGTCT TCTCACTCTT TGGCAACGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGAATGCACT CGGCCACCAG CCTCTTCATC GTCAACCTGG CAGTTGCCGA CATAATGATC 
ACGCTGCTCA ACACCCCCTT CACTTTGGTT CGCTTTGTGA ACAGCACATG GATATTTGGG 
AAGGGCATGT GCCATGTCAG CCGCTTTGCC CAGTACTGCT CACTGCACGT CTCAGCACTG 
ACACTGACAG CCATTGCGGT GGATCGCCAC CAGGTCATCA TGCACCCCTT GAAACCCCGG 
ATCTCAATCA CAAAGGGTGT CATCTACATC GCTGTCATCT GGACCATGGC TACGTTCTTT 
TCACTCCCAC ATGCTATCTG CCAGAAATTA TTTACCTTCA AATACAGTGA GGACATTGTG 
CGCTCCCTCT GCCTGCCAGA CTTCCCTGAG CCAGCTGACC TCTTCTGGAA GTACCTGGAC 
TTGGCCACCT TCATCCTGCT CTACATCCTG CCCCTCCTCA TCATCTCTGT GGCCTACGCT 
CGTGTGGCCA AGAAACTGTG GCTGTGTAAT ATGATTGGCG ATGTGACCAC AGAGCAGTAC 
TTTGCCCTGC GGCGCAAAAA GAAGAAGACC ATCAAGATGT TGATGCTGGT GGTAGTCCTC 
TTTGCCCTCT GCTGGTTCCC CCTCAACTGC TACGTCCTCC TCCTGTCCAG CAAGGTCATC 
CGCACCAACA ATGCCCTCTA CTTTGCCTTC CACTGGTTTG CCATGAGCAG CACCTGCTAT 
AACCCCTTCA TATACTGCTG GCTGAACGAG AACTTCAGGA TTGAGCTAAA GGCATTACTG 
AGCATGTGTC AAAGACCTCC CAAGCCTCAG GAGGACAGGC CACCCTCCCC AGTTCCTTCC 
TTCAGGGTGG CCTGGACAGA GAAGAATGAT GGCCAGAGGG CTCCCCTTGC CAATAACCTC 
CTGCCCACCT CCCAACTCCA GTCTGGGAAG ACAGACCTGT CATCTGTGGA ACCCATTGTG 
ACGATGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057624.3
CDS	173..1444
Translation	MVPHLLLLCLLPLVRATEPHEGRADEQSAEAALAVPNASHFFSWNNYTFSDWQNFVGRRRYGAESQNPTVKALLIVAYSFIIVFSLFGNVLVCHVIFKNQRMHSATSLFIVNLAVADIMITLLNTPFTLVRFVNSTWIFGKGMCHVSRFAQYCSLHVSALTLTAIAVDRHQVIMHPLKPRISITKGVIYIAVIWTMATFFSLPHAICQKLFTFKYSEDIVRSLCLPDFPEPADLFWKYLDLATFILLYILPLLIISVAYARVAKKLWLCNMIGDVTTEQYFALRRKKKKTIKMLMLVVVLFALCWFPLNCYVLLLSSKVIRTNNALYFAFHWFAMSSTCYNPFIYCWLNENFRIELKALLSMCQRPPKPQEDRPPSPVPSFRVAWTEKNDGQRAPLANNLLPTSQLQSGKTDLSSVEPIVTMS

Target ORF information:

RefSeq Version	NM_016540.3
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 83 (GPR83), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016540.3

ORF Insert Sequence:

ATGGTCCCTC ACCTCTTGCT GCTCTGTCTC CTCCCCTTGG TGCGAGCCAC CGAGCCCCAC 
GAGGGCCGGG CCGACGAGCA GAGCGCGGAG GCGGCCCTGG CCGTGCCCAA TGCCTCGCAC 
TTCTTCTCTT GGAACAACTA CACCTTCTCC GACTGGCAGA ACTTTGTGGG CAGGAGGCGC 
TACGGCGCTG AGTCCCAGAA CCCCACGGTG AAAGCCCTGC TCATTGTGGC TTACTCCTTC 
ATCATTGTCT TCTCACTCTT TGGCAACGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGAATGCACT CGGCCACCAG CCTCTTCATC GTCAACCTGG CAGTTGCCGA CATAATGATC 
ACGCTGCTCA ACACCCCCTT CACTTTGGTT CGCTTTGTGA ACAGCACATG GATATTTGGG 
AAGGGCATGT GCCATGTCAG CCGCTTTGCC CAGTACTGCT CACTGCACGT CTCAGCACTG 
ACACTGACAG CCATTGCGGT GGATCGCCAC CAGGTCATCA TGCACCCCTT GAAACCCCGG 
ATCTCAATCA CAAAGGGTGT CATCTACATC GCTGTCATCT GGACCATGGC TACGTTCTTT 
TCACTCCCAC ATGCTATCTG CCAGAAATTA TTTACCTTCA AATACAGTGA GGACATTGTG 
CGCTCCCTCT GCCTGCCAGA CTTCCCTGAG CCAGCTGACC TCTTCTGGAA GTACCTGGAC 
TTGGCCACCT TCATCCTGCT CTACATCCTG CCCCTCCTCA TCATCTCTGT GGCCTACGCT 
CGTGTGGCCA AGAAACTGTG GCTGTGTAAT ATGATTGGCG ATGTGACCAC AGAGCAGTAC 
TTTGCCCTGC GGCGCAAAAA GAAGAAGACC ATCAAGATGT TGATGCTGGT GGTAGTCCTC 
TTTGCCCTCT GCTGGTTCCC CCTCAACTGC TACGTCCTCC TCCTGTCCAG CAAGGTCATC 
CGCACCAACA ATGCCCTCTA CTTTGCCTTC CACTGGTTTG CCATGAGCAG CACCTGCTAT 
AACCCCTTCA TATACTGCTG GCTGAACGAG AACTTCAGGA TTGAGCTAAA GGCATTACTG 
AGCATGTGTC AAAGACCTCC CAAGCCTCAG GAGGACAGGC CACCCTCCCC AGTTCCTTCC 
TTCAGGGTGG CCTGGACAGA GAAGAATGAT GGCCAGAGGG CTCCCCTTGC CAATAACCTC 
CTGCCCACCT CCCAACTCCA GTCTGGGAAG ACAGACCTGT CATCTGTGGA ACCCATTGTG 
ACGATGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR83 ( NM_001330345.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317274.1 Homo sapiens G protein-coupled receptor 83 (GPR83), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu64485
Clone ID Related Accession (Same CDS sequence)	NM_001330345.1 , NM_001330345.2
Accession Version	NM_001330345.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	probable G-protein coupled receptor 83 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP000765.5 and BE670361.1. On Aug 23, 2016 this sequence version replaced XM_011542559.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.157511.1, SRR1803612.13127.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCCTC ACCTCTTGCT GCTCTGTCTC CTCCCCTTGG TGCGAGCCAC CGAGCCCCAC 
GAGGGCCGGG CCGACGAGCA GAGCGCGGAG GCGGCCCTGG CCGTGCCCAA TGCCTCGCAC 
TTCTTCTCTT GGAACAACTA CACCTTCTCC GACTGGCAGA ACTTTGTGGG CAGGAGGCGC 
TACGGCGCTG AGTCCCAGAA CCCCACGGTG AAAGCCCTGC TCATTGTGGC TTACTCCTTC 
ATCATTGTCT TCTCACTCTT TGGCAACGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGAATGCACT CGGCCACCAG CCTCTTCATC GTCAACCTGG CAGTTGCCGA CATAATGATC 
ACGCTGCTCA ACACCCCCTT CACTTTGGTC ATCATGCACC CCTTGAAACC CCGGATCTCA 
ATCACAAAGG GTGTCATCTA CATCGCTGTC ATCTGGACCA TGGCTACGTT CTTTTCACTC 
CCACATGCTA TCTGCCAGAA ATTATTTACC TTCAAATACA GTGAGGACAT TGTGCGCTCC 
CTCTGCCTGC CAGACTTCCC TGAGCCAGCT GACCTCTTCT GGAAGTACCT GGACTTGGCC 
ACCTTCATCC TGCTCTACAT CCTGCCCCTC CTCATCATCT CTGTGGCCTA CGCTCGTGTG 
GCCAAGAAAC TGTGGCTGTG TAATATGATT GGCGATGTGA CCACAGAGCA GTACTTTGCC 
CTGCGGCGCA AAAAGAAGAA GACCATCAAG ATGTTGATGC TGGTGGTAGT CCTCTTTGCC 
CTCTGCTGGT TCCCCCTCAA CTGCTACGTC CTCCTCCTGT CCAGCAAGGT CATCCGCACC 
AACAATGCCC TCTACTTTGC CTTCCACTGG TTTGCCATGA GCAGCACCTG CTATAACCCC 
TTCATATACT GCTGGCTGAA CGAGAACTTC AGGATTGAGC TAAAGGCATT ACTGAGCATG 
TGTCAAAGAC CTCCCAAGCC TCAGGAGGAC AGGCCACCCT CCCCAGTTCC TTCCTTCAGG 
GTGGCCTGGA CAGAGAAGAA TGATGGCCAG AGGGCTCCCC TTGCCAATAA CCTCCTGCCC 
ACCTCCCAAC TCCAGTCTGG GAAGACAGAC CTGTCATCTG TGGAACCCAT TGTGACGATG 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317274.1
CDS	177..1322
Translation	MVPHLLLLCLLPLVRATEPHEGRADEQSAEAALAVPNASHFFSWNNYTFSDWQNFVGRRRYGAESQNPTVKALLIVAYSFIIVFSLFGNVLVCHVIFKNQRMHSATSLFIVNLAVADIMITLLNTPFTLVIMHPLKPRISITKGVIYIAVIWTMATFFSLPHAICQKLFTFKYSEDIVRSLCLPDFPEPADLFWKYLDLATFILLYILPLLIISVAYARVAKKLWLCNMIGDVTTEQYFALRRKKKKTIKMLMLVVVLFALCWFPLNCYVLLLSSKVIRTNNALYFAFHWFAMSSTCYNPFIYCWLNENFRIELKALLSMCQRPPKPQEDRPPSPVPSFRVAWTEKNDGQRAPLANNLLPTSQLQSGKTDLSSVEPIVTMS

Target ORF information:

RefSeq Version	NM_001330345.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 83 (GPR83), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330345.1

ORF Insert Sequence:

ATGGTCCCTC ACCTCTTGCT GCTCTGTCTC CTCCCCTTGG TGCGAGCCAC CGAGCCCCAC 
GAGGGCCGGG CCGACGAGCA GAGCGCGGAG GCGGCCCTGG CCGTGCCCAA TGCCTCGCAC 
TTCTTCTCTT GGAACAACTA CACCTTCTCC GACTGGCAGA ACTTTGTGGG CAGGAGGCGC 
TACGGCGCTG AGTCCCAGAA CCCCACGGTG AAAGCCCTGC TCATTGTGGC TTACTCCTTC 
ATCATTGTCT TCTCACTCTT TGGCAACGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGAATGCACT CGGCCACCAG CCTCTTCATC GTCAACCTGG CAGTTGCCGA CATAATGATC 
ACGCTGCTCA ACACCCCCTT CACTTTGGTC ATCATGCACC CCTTGAAACC CCGGATCTCA 
ATCACAAAGG GTGTCATCTA CATCGCTGTC ATCTGGACCA TGGCTACGTT CTTTTCACTC 
CCACATGCTA TCTGCCAGAA ATTATTTACC TTCAAATACA GTGAGGACAT TGTGCGCTCC 
CTCTGCCTGC CAGACTTCCC TGAGCCAGCT GACCTCTTCT GGAAGTACCT GGACTTGGCC 
ACCTTCATCC TGCTCTACAT CCTGCCCCTC CTCATCATCT CTGTGGCCTA CGCTCGTGTG 
GCCAAGAAAC TGTGGCTGTG TAATATGATT GGCGATGTGA CCACAGAGCA GTACTTTGCC 
CTGCGGCGCA AAAAGAAGAA GACCATCAAG ATGTTGATGC TGGTGGTAGT CCTCTTTGCC 
CTCTGCTGGT TCCCCCTCAA CTGCTACGTC CTCCTCCTGT CCAGCAAGGT CATCCGCACC 
AACAATGCCC TCTACTTTGC CTTCCACTGG TTTGCCATGA GCAGCACCTG CTATAACCCC 
TTCATATACT GCTGGCTGAA CGAGAACTTC AGGATTGAGC TAAAGGCATT ACTGAGCATG 
TGTCAAAGAC CTCCCAAGCC TCAGGAGGAC AGGCCACCCT CCCCAGTTCC TTCCTTCAGG 
GTGGCCTGGA CAGAGAAGAA TGATGGCCAG AGGGCTCCCC TTGCCAATAA CCTCCTGCCC 
ACCTCCCAAC TCCAGTCTGG GAAGACAGAC CTGTCATCTG TGGAACCCAT TGTGACGATG 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPR83 ( NM_001330345.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317274.1 Homo sapiens G protein-coupled receptor 83 (GPR83), transcript variant 2, mRNA.

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CloneID	OHu64485
Clone ID Related Accession (Same CDS sequence)	NM_001330345.1 , NM_001330345.2
Accession Version	NM_001330345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	probable G-protein coupled receptor 83 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP000765.5. On May 31, 2019 this sequence version replaced NM_001330345.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.13127.1, SRR1660803.157511.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCCCTC ACCTCTTGCT GCTCTGTCTC CTCCCCTTGG TGCGAGCCAC CGAGCCCCAC 
GAGGGCCGGG CCGACGAGCA GAGCGCGGAG GCGGCCCTGG CCGTGCCCAA TGCCTCGCAC 
TTCTTCTCTT GGAACAACTA CACCTTCTCC GACTGGCAGA ACTTTGTGGG CAGGAGGCGC 
TACGGCGCTG AGTCCCAGAA CCCCACGGTG AAAGCCCTGC TCATTGTGGC TTACTCCTTC 
ATCATTGTCT TCTCACTCTT TGGCAACGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGAATGCACT CGGCCACCAG CCTCTTCATC GTCAACCTGG CAGTTGCCGA CATAATGATC 
ACGCTGCTCA ACACCCCCTT CACTTTGGTC ATCATGCACC CCTTGAAACC CCGGATCTCA 
ATCACAAAGG GTGTCATCTA CATCGCTGTC ATCTGGACCA TGGCTACGTT CTTTTCACTC 
CCACATGCTA TCTGCCAGAA ATTATTTACC TTCAAATACA GTGAGGACAT TGTGCGCTCC 
CTCTGCCTGC CAGACTTCCC TGAGCCAGCT GACCTCTTCT GGAAGTACCT GGACTTGGCC 
ACCTTCATCC TGCTCTACAT CCTGCCCCTC CTCATCATCT CTGTGGCCTA CGCTCGTGTG 
GCCAAGAAAC TGTGGCTGTG TAATATGATT GGCGATGTGA CCACAGAGCA GTACTTTGCC 
CTGCGGCGCA AAAAGAAGAA GACCATCAAG ATGTTGATGC TGGTGGTAGT CCTCTTTGCC 
CTCTGCTGGT TCCCCCTCAA CTGCTACGTC CTCCTCCTGT CCAGCAAGGT CATCCGCACC 
AACAATGCCC TCTACTTTGC CTTCCACTGG TTTGCCATGA GCAGCACCTG CTATAACCCC 
TTCATATACT GCTGGCTGAA CGAGAACTTC AGGATTGAGC TAAAGGCATT ACTGAGCATG 
TGTCAAAGAC CTCCCAAGCC TCAGGAGGAC AGGCCACCCT CCCCAGTTCC TTCCTTCAGG 
GTGGCCTGGA CAGAGAAGAA TGATGGCCAG AGGGCTCCCC TTGCCAATAA CCTCCTGCCC 
ACCTCCCAAC TCCAGTCTGG GAAGACAGAC CTGTCATCTG TGGAACCCAT TGTGACGATG 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317274.1
CDS	173..1318
Translation	MVPHLLLLCLLPLVRATEPHEGRADEQSAEAALAVPNASHFFSWNNYTFSDWQNFVGRRRYGAESQNPTVKALLIVAYSFIIVFSLFGNVLVCHVIFKNQRMHSATSLFIVNLAVADIMITLLNTPFTLVIMHPLKPRISITKGVIYIAVIWTMATFFSLPHAICQKLFTFKYSEDIVRSLCLPDFPEPADLFWKYLDLATFILLYILPLLIISVAYARVAKKLWLCNMIGDVTTEQYFALRRKKKKTIKMLMLVVVLFALCWFPLNCYVLLLSSKVIRTNNALYFAFHWFAMSSTCYNPFIYCWLNENFRIELKALLSMCQRPPKPQEDRPPSPVPSFRVAWTEKNDGQRAPLANNLLPTSQLQSGKTDLSSVEPIVTMS

Target ORF information:

RefSeq Version	NM_001330345.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor 83 (GPR83), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330345.2

ORF Insert Sequence:

ATGGTCCCTC ACCTCTTGCT GCTCTGTCTC CTCCCCTTGG TGCGAGCCAC CGAGCCCCAC 
GAGGGCCGGG CCGACGAGCA GAGCGCGGAG GCGGCCCTGG CCGTGCCCAA TGCCTCGCAC 
TTCTTCTCTT GGAACAACTA CACCTTCTCC GACTGGCAGA ACTTTGTGGG CAGGAGGCGC 
TACGGCGCTG AGTCCCAGAA CCCCACGGTG AAAGCCCTGC TCATTGTGGC TTACTCCTTC 
ATCATTGTCT TCTCACTCTT TGGCAACGTC CTGGTCTGTC ATGTCATCTT CAAGAACCAG 
CGAATGCACT CGGCCACCAG CCTCTTCATC GTCAACCTGG CAGTTGCCGA CATAATGATC 
ACGCTGCTCA ACACCCCCTT CACTTTGGTC ATCATGCACC CCTTGAAACC CCGGATCTCA 
ATCACAAAGG GTGTCATCTA CATCGCTGTC ATCTGGACCA TGGCTACGTT CTTTTCACTC 
CCACATGCTA TCTGCCAGAA ATTATTTACC TTCAAATACA GTGAGGACAT TGTGCGCTCC 
CTCTGCCTGC CAGACTTCCC TGAGCCAGCT GACCTCTTCT GGAAGTACCT GGACTTGGCC 
ACCTTCATCC TGCTCTACAT CCTGCCCCTC CTCATCATCT CTGTGGCCTA CGCTCGTGTG 
GCCAAGAAAC TGTGGCTGTG TAATATGATT GGCGATGTGA CCACAGAGCA GTACTTTGCC 
CTGCGGCGCA AAAAGAAGAA GACCATCAAG ATGTTGATGC TGGTGGTAGT CCTCTTTGCC 
CTCTGCTGGT TCCCCCTCAA CTGCTACGTC CTCCTCCTGT CCAGCAAGGT CATCCGCACC 
AACAATGCCC TCTACTTTGC CTTCCACTGG TTTGCCATGA GCAGCACCTG CTATAACCCC 
TTCATATACT GCTGGCTGAA CGAGAACTTC AGGATTGAGC TAAAGGCATT ACTGAGCATG 
TGTCAAAGAC CTCCCAAGCC TCAGGAGGAC AGGCCACCCT CCCCAGTTCC TTCCTTCAGG 
GTGGCCTGGA CAGAGAAGAA TGATGGCCAG AGGGCTCCCC TTGCCAATAA CCTCCTGCCC 
ACCTCCCAAC TCCAGTCTGG GAAGACAGAC CTGTCATCTG TGGAACCCAT TGTGACGATG 
AGTTAG