P3H4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	P3H4
Entrez Gene ID	10609
Full Name	prolyl 3-hydroxylase family member 4 (non-enzymatic)
Synonyms	LEPREL4,NO55,NOL55,SC65
General protein information	Preferred Names prolyl 3-hydroxylase family member 4 (non-enzymatic) Names endoplasmic reticulum protein SC65 synaptonemal complex protein SC65 leprecan-like 4 leprecan-like protein 4 nucleolar autoantigen (55kD) nucleolar autoantigen No55 nucleolar autoantigen, 55kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.2
Summary	This nucleolar protein was first characterized because it was an autoantigen in cases on interstitial cystitis. The protein, with a predicted molecular weight of 50 kDa, appears to be localized in the particulate compartment of the interphase nucleolus, with a distribution distinct from that of nucleolar protein B23. During mitosis it is associated with chromosomes. [provided by RefSeq, Jul 2008].
Disorder MIM:	617419

mRNA and Protein(s)

mRNA	Protein	Name
NM_006455.2	NP_006446.1	endoplasmic reticulum protein SC65 precursor
NM_006455.3	NP_006446.1	endoplasmic reticulum protein SC65 precursor
XM_024450538.1	XP_024306306.1	endoplasmic reticulum protein SC65 isoform X2
XM_006721640.3	XP_006721703.1	synaptonemal complex protein SC65 isoform X1
XM_006721640.4	XP_006721703.1	endoplasmic reticulum protein SC65 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LEPREL4	XP_001144364.2
Canis lupus familiaris (dog)	LEPREL4	XP_849608.1
Danio rerio (zebrafish)	leprel4	NP_001119910.1
Rattus norvegicus (Norway rat)	Leprel4	NP_067592.1
Xenopus tropicalis (tropical clawed frog)	leprel4	NP_001025630.1
	LEPREL4	XP_001093403.1
Gallus gallus (chicken)	LOC430099	XP_427657.4
Homo sapiens (human)	LEPREL4	NP_006446.1
Mus musculus (house mouse)	Leprel4	NP_789800.1

Gene Ontology
Bibliography

Related articles in PubMed

P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA.
Hudson DM, Weis M, Rai J, Joeng KS, Dimori M, Lee BH, Morello R, Eyre DR
The Journal of biological chemistry292(9)3877-3887(2017 03)

Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis.
Gruenwald K, Castagnola P, Besio R, Dimori M, Chen Y, Akel NS, Swain FL, Skinner RA, Eyre DR, Gaddy D, Suva LJ, Morello R
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research29(3)666-75(2014 Mar)

Identification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cDNA library.
Cavazzini F, Magistroni R, Furci L, Lupo V, Ligabue G, Granito M, Leonelli M, Albertazzi A, Cappelli G
PloS one7(11)e48845(2012)

Insulin-dependent interactions of proteins with GLUT4 revealed through stable isotope labeling by amino acids in cell culture (SILAC).
Foster LJ, Rudich A, Talior I, Patel N, Huang X, Furtado LM, Bilan PJ, Mann M, Klip A
Journal of proteome research5(1)64-75(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SC65 protein is identified as an autoimmune target in idiopathic membranous nephropathy.
Title: Identification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cDNA library.

The following P3H4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P3H4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05491	NM_006455.3 Latest version!	Homo sapiens prolyl 3-hydroxylase family member 4 (inactive) (P3H4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu104233	XM_024450538.1 Latest version!	Homo sapiens prolyl 3-hydroxylase family member 4 (inactive) (P3H4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu50033	XM_006721640.4 Latest version!	Homo sapiens prolyl 3-hydroxylase family member 4 (inactive) (P3H4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu05491	NM_006455.2	Homo sapiens prolyl 3-hydroxylase family member 4 (non-enzymatic) (P3H4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu50033	XM_006721640.3	Homo sapiens prolyl 3-hydroxylase family member 4 (non-enzymatic) (P3H4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P3H4 ( NM_006455.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006446.1 Homo sapiens prolyl 3-hydroxylase family member 4 (non-enzymatic) (P3H4), mRNA.

Price & Availability↑

CloneID	OHu05491
Clone ID Related Accession (Same CDS sequence)	NM_006455.2 , NM_006455.3
Accession Version	NM_006455.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Homo sapiens(human)
Product	endoplasmic reticulum protein SC65 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM834302.1, AK056085.1, BC007942.2 and U47621.1. On Dec 12, 2003 this sequence version replaced NM_006455.1. ##Evidence-Data-START## Transcript exon combination :: U47621.1, AJ250583.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCGGG TGGCGTGGGG GCTGCTGTGG TTGCTGCTGG GCAGCGCCGG GGCGCAGTAC 
GAGAAGTACA GCTTCCGGGG CTTCCCGCCC GAGGACCTGA TGCCGCTGGC CGCGGCGTAC 
GGGCACGCTC TGGAGCAGTA CGAGGGAGAG AGCTGGCGCG AGAGCGCGCG CTACCTGGAG 
GCGGCGCTGC GGCTGCACCG GCTCCTGCGC GACAGCGAGG CCTTCTGCCA CGCCAACTGC 
AGCGGCCCCG CGCCCGCGGC CAAGCCCGAT CCCGACGGCG GCCGCGCAGA CGAGTGGGCC 
TGCGAGCTGC GGCTCTTCGG CCGCGTCCTG GAGCGAGCCG CCTGCCTGCG GCGCTGCAAG 
CGGACGCTGC CCGCCTTCCA GGTGCCCTAC CCGCCGCGGC AGCTGCTGCG TGACTTCCAG 
AGCCGCCTGC CCTACCAGTA CCTGCACTAC GCGCTGTTCA AGGCTAACCG GCTGGAGAAG 
GCGGTGGCGG CGGCCTACAC CTTCCTCCAG AGGAACCCGA AGCACGAGCT GACCGCCAAG 
TATCTCAACT ACTATCAGGG GATGCTGGAC GTCGCCGACG AGTCCCTCAC GGACCTAGAG 
GCCCAGCCCT ACGAGGCCGT GTTCCTCCGG GCTGTGAAGC TCTACAACAG CGGGGATTTC 
CGCAGCAGCA CGGAGGACAT GGAGCGGGCC TTGTCAGAGT ACCTGGCAGT CTTTGCCCGG 
TGCCTGGCCG GCTGTGAAGG GGCCCATGAG CAGGTGGACT TCAAGGACTT CTACCCGGCC 
ATAGCAGATC TCTTTGCAGA GTCCCTGCAG TGCAAGGTGG ACTGTGAGGC CAATTTGACC 
CCCAATGTGG GTGGCTACTT CGTGGACAAG TTCGTGGCCA CCATGTACCA CTACCTGCAG 
TTTGCCTACT ATAAGTTGAA TGATGTGCGC CAGGCTGCCC GCAGCGCCGC CAGCTACATG 
CTCTTCGACC CCAAGGACAG CGTCATGCAG CAGAACCTGG TGTATTACCG GTTCCACCGG 
GCTCGCTGGG GCCTGGAAGA GGAGGACTTC CAGCCCCGGG AGGAGGCCAT GCTCTACCAC 
AACCAGACCG CCGAGCTGCG GGAGCTGCTG GAGTTCACCC ACATGTACCT GCAGTCAGAT 
GATGAGATGG AGCTGGAGGA GACAGAACCG CCCCTGGAGC CTGAGGATGC CCTATCTGAC 
GCCGAGTTTG AGGGGGAGGG TGACTACGAG GAGGGCATGT ATGCTGACTG GTGGCAGGAG 
CCGGATGCCA AGGGTGACGA GGCCGAGGCT GAGCCAGAGC CTGAACTCGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006446.1
CDS	285..1598
Translation	MARVAWGLLWLLLGSAGAQYEKYSFRGFPPEDLMPLAAAYGHALEQYEGESWRESARYLEAALRLHRLLRDSEAFCHANCSGPAPAAKPDPDGGRADEWACELRLFGRVLERAACLRRCKRTLPAFQVPYPPRQLLRDFQSRLPYQYLHYALFKANRLEKAVAAAYTFLQRNPKHELTAKYLNYYQGMLDVADESLTDLEAQPYEAVFLRAVKLYNSGDFRSSTEDMERALSEYLAVFARCLAGCEGAHEQVDFKDFYPAIADLFAESLQCKVDCEANLTPNVGGYFVDKFVATMYHYLQFAYYKLNDVRQAARSAASYMLFDPKDSVMQQNLVYYRFHRARWGLEEEDFQPREEAMLYHNQTAELRELLEFTHMYLQSDDEMELEETEPPLEPEDALSDAEFEGEGDYEEGMYADWWQEPDAKGDEAEAEPEPELA

Target ORF information:

RefSeq Version	NM_006455.2
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 3-hydroxylase family member 4 (non-enzymatic) (P3H4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006455.2

ORF Insert Sequence:

ATGGCTCGGG TGGCGTGGGG GCTGCTGTGG TTGCTGCTGG GCAGCGCCGG GGCGCAGTAC 
GAGAAGTACA GCTTCCGGGG CTTCCCGCCC GAGGACCTGA TGCCGCTGGC CGCGGCGTAC 
GGGCACGCTC TGGAGCAGTA CGAGGGAGAG AGCTGGCGCG AGAGCGCGCG CTACCTGGAG 
GCGGCGCTGC GGCTGCACCG GCTCCTGCGC GACAGCGAGG CCTTCTGCCA CGCCAACTGC 
AGCGGCCCCG CGCCCGCGGC CAAGCCCGAT CCCGACGGCG GCCGCGCAGA CGAGTGGGCC 
TGCGAGCTGC GGCTCTTCGG CCGCGTCCTG GAGCGAGCCG CCTGCCTGCG GCGCTGCAAG 
CGGACGCTGC CCGCCTTCCA GGTGCCCTAC CCGCCGCGGC AGCTGCTGCG TGACTTCCAG 
AGCCGCCTGC CCTACCAGTA CCTGCACTAC GCGCTGTTCA AGGCTAACCG GCTGGAGAAG 
GCGGTGGCGG CGGCCTACAC CTTCCTCCAG AGGAACCCGA AGCACGAGCT GACCGCCAAG 
TATCTCAACT ACTATCAGGG GATGCTGGAC GTCGCCGACG AGTCCCTCAC GGACCTAGAG 
GCCCAGCCCT ACGAGGCCGT GTTCCTCCGG GCTGTGAAGC TCTACAACAG CGGGGATTTC 
CGCAGCAGCA CGGAGGACAT GGAGCGGGCC TTGTCAGAGT ACCTGGCAGT CTTTGCCCGG 
TGCCTGGCCG GCTGTGAAGG GGCCCATGAG CAGGTGGACT TCAAGGACTT CTACCCGGCC 
ATAGCAGATC TCTTTGCAGA GTCCCTGCAG TGCAAGGTGG ACTGTGAGGC CAATTTGACC 
CCCAATGTGG GTGGCTACTT CGTGGACAAG TTCGTGGCCA CCATGTACCA CTACCTGCAG 
TTTGCCTACT ATAAGTTGAA TGATGTGCGC CAGGCTGCCC GCAGCGCCGC CAGCTACATG 
CTCTTCGACC CCAAGGACAG CGTCATGCAG CAGAACCTGG TGTATTACCG GTTCCACCGG 
GCTCGCTGGG GCCTGGAAGA GGAGGACTTC CAGCCCCGGG AGGAGGCCAT GCTCTACCAC 
AACCAGACCG CCGAGCTGCG GGAGCTGCTG GAGTTCACCC ACATGTACCT GCAGTCAGAT 
GATGAGATGG AGCTGGAGGA GACAGAACCG CCCCTGGAGC CTGAGGATGC CCTATCTGAC 
GCCGAGTTTG AGGGGGAGGG TGACTACGAG GAGGGCATGT ATGCTGACTG GTGGCAGGAG 
CCGGATGCCA AGGGTGACGA GGCCGAGGCT GAGCCAGAGC CTGAACTCGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3H4 ( NM_006455.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006446.1 Homo sapiens prolyl 3-hydroxylase family member 4 (inactive) (P3H4), mRNA.

Price & Availability↑

CloneID	OHu05491
Clone ID Related Accession (Same CDS sequence)	NM_006455.2 , NM_006455.3
Accession Version	NM_006455.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	endoplasmic reticulum protein SC65 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK056085.1, BC007942.2 and U47621.1. On Nov 22, 2018 this sequence version replaced NM_006455.2. ##Evidence-Data-START## Transcript exon combination :: U47621.1, AJ250583.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000393928.6/ ENSP00000377505.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCGGG TGGCGTGGGG GCTGCTGTGG TTGCTGCTGG GCAGCGCCGG GGCGCAGTAC 
GAGAAGTACA GCTTCCGGGG CTTCCCGCCC GAGGACCTGA TGCCGCTGGC CGCGGCGTAC 
GGGCACGCTC TGGAGCAGTA CGAGGGAGAG AGCTGGCGCG AGAGCGCGCG CTACCTGGAG 
GCGGCGCTGC GGCTGCACCG GCTCCTGCGC GACAGCGAGG CCTTCTGCCA CGCCAACTGC 
AGCGGCCCCG CGCCCGCGGC CAAGCCCGAT CCCGACGGCG GCCGCGCAGA CGAGTGGGCC 
TGCGAGCTGC GGCTCTTCGG CCGCGTCCTG GAGCGAGCCG CCTGCCTGCG GCGCTGCAAG 
CGGACGCTGC CCGCCTTCCA GGTGCCCTAC CCGCCGCGGC AGCTGCTGCG TGACTTCCAG 
AGCCGCCTGC CCTACCAGTA CCTGCACTAC GCGCTGTTCA AGGCTAACCG GCTGGAGAAG 
GCGGTGGCGG CGGCCTACAC CTTCCTCCAG AGGAACCCGA AGCACGAGCT GACCGCCAAG 
TATCTCAACT ACTATCAGGG GATGCTGGAC GTCGCCGACG AGTCCCTCAC GGACCTAGAG 
GCCCAGCCCT ACGAGGCCGT GTTCCTCCGG GCTGTGAAGC TCTACAACAG CGGGGATTTC 
CGCAGCAGCA CGGAGGACAT GGAGCGGGCC TTGTCAGAGT ACCTGGCAGT CTTTGCCCGG 
TGCCTGGCCG GCTGTGAAGG GGCCCATGAG CAGGTGGACT TCAAGGACTT CTACCCGGCC 
ATAGCAGATC TCTTTGCAGA GTCCCTGCAG TGCAAGGTGG ACTGTGAGGC CAATTTGACC 
CCCAATGTGG GTGGCTACTT CGTGGACAAG TTCGTGGCCA CCATGTACCA CTACCTGCAG 
TTTGCCTACT ATAAGTTGAA TGATGTGCGC CAGGCTGCCC GCAGCGCCGC CAGCTACATG 
CTCTTCGACC CCAAGGACAG CGTCATGCAG CAGAACCTGG TGTATTACCG GTTCCACCGG 
GCTCGCTGGG GCCTGGAAGA GGAGGACTTC CAGCCCCGGG AGGAGGCCAT GCTCTACCAC 
AACCAGACCG CCGAGCTGCG GGAGCTGCTG GAGTTCACCC ACATGTACCT GCAGTCAGAT 
GATGAGATGG AGCTGGAGGA GACAGAACCG CCCCTGGAGC CTGAGGATGC CCTATCTGAC 
GCCGAGTTTG AGGGGGAGGG TGACTACGAG GAGGGCATGT ATGCTGACTG GTGGCAGGAG 
CCGGATGCCA AGGGTGACGA GGCCGAGGCT GAGCCAGAGC CTGAACTCGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006446.1
CDS	35..1348
Translation	MARVAWGLLWLLLGSAGAQYEKYSFRGFPPEDLMPLAAAYGHALEQYEGESWRESARYLEAALRLHRLLRDSEAFCHANCSGPAPAAKPDPDGGRADEWACELRLFGRVLERAACLRRCKRTLPAFQVPYPPRQLLRDFQSRLPYQYLHYALFKANRLEKAVAAAYTFLQRNPKHELTAKYLNYYQGMLDVADESLTDLEAQPYEAVFLRAVKLYNSGDFRSSTEDMERALSEYLAVFARCLAGCEGAHEQVDFKDFYPAIADLFAESLQCKVDCEANLTPNVGGYFVDKFVATMYHYLQFAYYKLNDVRQAARSAASYMLFDPKDSVMQQNLVYYRFHRARWGLEEEDFQPREEAMLYHNQTAELRELLEFTHMYLQSDDEMELEETEPPLEPEDALSDAEFEGEGDYEEGMYADWWQEPDAKGDEAEAEPEPELA

Target ORF information:

RefSeq Version	NM_006455.3
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 3-hydroxylase family member 4 (inactive) (P3H4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006455.3

ORF Insert Sequence:

ATGGCTCGGG TGGCGTGGGG GCTGCTGTGG TTGCTGCTGG GCAGCGCCGG GGCGCAGTAC 
GAGAAGTACA GCTTCCGGGG CTTCCCGCCC GAGGACCTGA TGCCGCTGGC CGCGGCGTAC 
GGGCACGCTC TGGAGCAGTA CGAGGGAGAG AGCTGGCGCG AGAGCGCGCG CTACCTGGAG 
GCGGCGCTGC GGCTGCACCG GCTCCTGCGC GACAGCGAGG CCTTCTGCCA CGCCAACTGC 
AGCGGCCCCG CGCCCGCGGC CAAGCCCGAT CCCGACGGCG GCCGCGCAGA CGAGTGGGCC 
TGCGAGCTGC GGCTCTTCGG CCGCGTCCTG GAGCGAGCCG CCTGCCTGCG GCGCTGCAAG 
CGGACGCTGC CCGCCTTCCA GGTGCCCTAC CCGCCGCGGC AGCTGCTGCG TGACTTCCAG 
AGCCGCCTGC CCTACCAGTA CCTGCACTAC GCGCTGTTCA AGGCTAACCG GCTGGAGAAG 
GCGGTGGCGG CGGCCTACAC CTTCCTCCAG AGGAACCCGA AGCACGAGCT GACCGCCAAG 
TATCTCAACT ACTATCAGGG GATGCTGGAC GTCGCCGACG AGTCCCTCAC GGACCTAGAG 
GCCCAGCCCT ACGAGGCCGT GTTCCTCCGG GCTGTGAAGC TCTACAACAG CGGGGATTTC 
CGCAGCAGCA CGGAGGACAT GGAGCGGGCC TTGTCAGAGT ACCTGGCAGT CTTTGCCCGG 
TGCCTGGCCG GCTGTGAAGG GGCCCATGAG CAGGTGGACT TCAAGGACTT CTACCCGGCC 
ATAGCAGATC TCTTTGCAGA GTCCCTGCAG TGCAAGGTGG ACTGTGAGGC CAATTTGACC 
CCCAATGTGG GTGGCTACTT CGTGGACAAG TTCGTGGCCA CCATGTACCA CTACCTGCAG 
TTTGCCTACT ATAAGTTGAA TGATGTGCGC CAGGCTGCCC GCAGCGCCGC CAGCTACATG 
CTCTTCGACC CCAAGGACAG CGTCATGCAG CAGAACCTGG TGTATTACCG GTTCCACCGG 
GCTCGCTGGG GCCTGGAAGA GGAGGACTTC CAGCCCCGGG AGGAGGCCAT GCTCTACCAC 
AACCAGACCG CCGAGCTGCG GGAGCTGCTG GAGTTCACCC ACATGTACCT GCAGTCAGAT 
GATGAGATGG AGCTGGAGGA GACAGAACCG CCCCTGGAGC CTGAGGATGC CCTATCTGAC 
GCCGAGTTTG AGGGGGAGGG TGACTACGAG GAGGGCATGT ATGCTGACTG GTGGCAGGAG 
CCGGATGCCA AGGGTGACGA GGCCGAGGCT GAGCCAGAGC CTGAACTCGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3H4 ( XM_024450538.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306306.1 Homo sapiens prolyl 3-hydroxylase family member 4 (inactive) (P3H4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu104233
Clone ID Related Accession (Same CDS sequence)	XM_024450538.1
Accession Version	XM_024450538.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoplasmic reticulum protein SC65 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGG GGGTGGTGGC AGCGCAAGGT GTGCAGGTGA CAGCAGGGGA CACCCCCAAG 
GCAGGAGCCC GGGCCGACGG AGAGGACTTA ACGACACTAT CGGACCCTCT GGGAAAAGAG 
GGGAGACGTC GTGACCCAGG CCCCGCCCCA CCTTGCCGCC TCGTGCCCGG CGCTAAGACC 
CAGCGGGCGC GCCGCCCGCC CGGGGCCCGG CCCTGTCCCC TTCCGTCCGC GGGGCAGCCA 
GCTCAGCTCC GGAGAGCCGG CGGCGCGGCG GGCATGGCTC GGGTGGCGTG GGGGCTGCTG 
TGGTTGCTGC TGGGCAGCGC CGGGGCGCAG TACGAGAAGT ACAGCTTCCG GGGCTTCCCG 
CCCGAGGACC TGATGCCGCT GGCCGCGGCG TACGGGCACG CTCTGGAGCA GTACGAGGGA 
GAGAGCTGGC GCGAGAGCGC GCGCTACCTG GAGGCGGCGC TGCGGCTGCA CCGGCTCCTG 
CGCGACAGCG AGGCCTTCTG CCACGCCAAC TGCAGCGGCC CCGCGCCCGC GGCCAAGCCC 
GATCCCGACG GCGGCCGCGC AGACGAGTGG GCCTGCGAGC TGCGGCTCTT CGGCCGCGTC 
CTGGAGCGAG CCGCCTGCCT GCGGCGCTGC AAGCGGACGC TGCCCGCCTT CCAGGTGCCC 
TACCCGCCGC GGCAGCTGCT GCGTGACTTC CAGAGCCGCC TGCCCTACCA GTACCTGCAC 
TACGCGCTGT TCAAGGCTAA CCGGCTGGAG AAGGCGGTGG CGGCGGCCTA CACCTTCCTC 
CAGAGGAACC CGAAGCACGA GCTGACCGCC AAGTATCTCA ACTACTATCA GGGGATGCTG 
GACGTCGCCG ACGAGTCCCT CACGGACCTA GAGGCCCAGC CCTACGAGGC CGTGTTCCTC 
CGGGCTGTGA AGCTCTACAA CAGCGGGGAT TTCCGCAGCA GCACGGAGGA CATGGAGCGG 
GCCTTGTCAG AGTACCTGGC AGTCTTTGCC CGGTGCCTGG CCGGCTGTGA AGGGGCCCAT 
GAGCAGGTGG ACTTCAAGGA CTTCTACCCG GCCATAGCAG ATCTCTTTGC AGAGTCCCTG 
CAGTGCAAGG TGGACTGTGA GGCCAATTTG ACCCCCAATG TGGGTGGCTA CTTCGTGGAC 
AAGTTCGTGG CCACCATGTA CCACTACCTG CAGTTTGCCT ACTATAAGTT GAATGATGTG 
CGCCAGGCTG CCCGCAGCGC CGCCAGCTAC ATGCTCTTCG ACCCCAAGGA CAGCGTCATG 
CAGCAGAACC TGGTGTATTA CCGGTTCCAC CGGGCTCGCT GGGGCCTGGA AGAGGAGGAC 
TTCCAGCCCC GGGAGGAGGC CATGCTCTAC CACAACCAGA CCGCCGAGCT GCGGGAGCTG 
CTGGAGTTCA CCCACATGTA CCTGCAGTCA GATGATGAGA TGGAGCTGGA GGAGACAGAA 
CCGCCCCTGG AGCCTGAGGA TGCCCTATCT GACGCCGAGT TTGAGGGGGA GGGTGACTAC 
GAGGAGGGCA TGTATGCTGA CTGGTGGCAG GAGCCGGATG CCAAGGGTGA CGAGGCCGAG 
GCTGAGCCAG AGCCTGAACT CGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306306.1
CDS	194..1780
Translation	MEWGVVAAQGVQVTAGDTPKAGARADGEDLTTLSDPLGKEGRRRDPGPAPPCRLVPGAKTQRARRPPGARPCPLPSAGQPAQLRRAGGAAGMARVAWGLLWLLLGSAGAQYEKYSFRGFPPEDLMPLAAAYGHALEQYEGESWRESARYLEAALRLHRLLRDSEAFCHANCSGPAPAAKPDPDGGRADEWACELRLFGRVLERAACLRRCKRTLPAFQVPYPPRQLLRDFQSRLPYQYLHYALFKANRLEKAVAAAYTFLQRNPKHELTAKYLNYYQGMLDVADESLTDLEAQPYEAVFLRAVKLYNSGDFRSSTEDMERALSEYLAVFARCLAGCEGAHEQVDFKDFYPAIADLFAESLQCKVDCEANLTPNVGGYFVDKFVATMYHYLQFAYYKLNDVRQAARSAASYMLFDPKDSVMQQNLVYYRFHRARWGLEEEDFQPREEAMLYHNQTAELRELLEFTHMYLQSDDEMELEETEPPLEPEDALSDAEFEGEGDYEEGMYADWWQEPDAKGDEAEAEPEPELA

Target ORF information:

RefSeq Version	XM_024450538.1
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 3-hydroxylase family member 4 (inactive) (P3H4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450538.1

ORF Insert Sequence:

ATGGAGTGGG GGGTGGTGGC AGCGCAAGGT GTGCAGGTGA CAGCAGGGGA CACCCCCAAG 
GCAGGAGCCC GGGCCGACGG AGAGGACTTA ACGACACTAT CGGACCCTCT GGGAAAAGAG 
GGGAGACGTC GTGACCCAGG CCCCGCCCCA CCTTGCCGCC TCGTGCCCGG CGCTAAGACC 
CAGCGGGCGC GCCGCCCGCC CGGGGCCCGG CCCTGTCCCC TTCCGTCCGC GGGGCAGCCA 
GCTCAGCTCC GGAGAGCCGG CGGCGCGGCG GGCATGGCTC GGGTGGCGTG GGGGCTGCTG 
TGGTTGCTGC TGGGCAGCGC CGGGGCGCAG TACGAGAAGT ACAGCTTCCG GGGCTTCCCG 
CCCGAGGACC TGATGCCGCT GGCCGCGGCG TACGGGCACG CTCTGGAGCA GTACGAGGGA 
GAGAGCTGGC GCGAGAGCGC GCGCTACCTG GAGGCGGCGC TGCGGCTGCA CCGGCTCCTG 
CGCGACAGCG AGGCCTTCTG CCACGCCAAC TGCAGCGGCC CCGCGCCCGC GGCCAAGCCC 
GATCCCGACG GCGGCCGCGC AGACGAGTGG GCCTGCGAGC TGCGGCTCTT CGGCCGCGTC 
CTGGAGCGAG CCGCCTGCCT GCGGCGCTGC AAGCGGACGC TGCCCGCCTT CCAGGTGCCC 
TACCCGCCGC GGCAGCTGCT GCGTGACTTC CAGAGCCGCC TGCCCTACCA GTACCTGCAC 
TACGCGCTGT TCAAGGCTAA CCGGCTGGAG AAGGCGGTGG CGGCGGCCTA CACCTTCCTC 
CAGAGGAACC CGAAGCACGA GCTGACCGCC AAGTATCTCA ACTACTATCA GGGGATGCTG 
GACGTCGCCG ACGAGTCCCT CACGGACCTA GAGGCCCAGC CCTACGAGGC CGTGTTCCTC 
CGGGCTGTGA AGCTCTACAA CAGCGGGGAT TTCCGCAGCA GCACGGAGGA CATGGAGCGG 
GCCTTGTCAG AGTACCTGGC AGTCTTTGCC CGGTGCCTGG CCGGCTGTGA AGGGGCCCAT 
GAGCAGGTGG ACTTCAAGGA CTTCTACCCG GCCATAGCAG ATCTCTTTGC AGAGTCCCTG 
CAGTGCAAGG TGGACTGTGA GGCCAATTTG ACCCCCAATG TGGGTGGCTA CTTCGTGGAC 
AAGTTCGTGG CCACCATGTA CCACTACCTG CAGTTTGCCT ACTATAAGTT GAATGATGTG 
CGCCAGGCTG CCCGCAGCGC CGCCAGCTAC ATGCTCTTCG ACCCCAAGGA CAGCGTCATG 
CAGCAGAACC TGGTGTATTA CCGGTTCCAC CGGGCTCGCT GGGGCCTGGA AGAGGAGGAC 
TTCCAGCCCC GGGAGGAGGC CATGCTCTAC CACAACCAGA CCGCCGAGCT GCGGGAGCTG 
CTGGAGTTCA CCCACATGTA CCTGCAGTCA GATGATGAGA TGGAGCTGGA GGAGACAGAA 
CCGCCCCTGG AGCCTGAGGA TGCCCTATCT GACGCCGAGT TTGAGGGGGA GGGTGACTAC 
GAGGAGGGCA TGTATGCTGA CTGGTGGCAG GAGCCGGATG CCAAGGGTGA CGAGGCCGAG 
GCTGAGCCAG AGCCTGAACT CGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3H4 ( XM_006721640.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006721703.1 Homo sapiens prolyl 3-hydroxylase family member 4 (non-enzymatic) (P3H4), transcript variant X1, mRNA.

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CloneID	OHu50033
Clone ID Related Accession (Same CDS sequence)	XM_006721640.3 , XM_006721640.4
Accession Version	XM_006721640.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	synaptonemal complex protein SC65 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721640.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGGG GCTTCCTGGG CTTCCCATCT CTGGCGGGAA GCGCTCCCCG ACGCATTCTC 
TACCTAGGGG ACACCCCCAA GGCAGGAGCC CGGGCCGACG GAGAGGACTT AACGACACTA 
TCGGACCCTC TGGGAAAAGA GGGGAGACGT CGTGACCCAG GCCCCGCCCC ACCTTGCCGC 
CTCGTGCCCG GCGCTAAGAC CCAGCGGGCG CGCCGCCCGC CCGGGGCCCG GCCCTGTCCC 
CTTCCGTCCG CGGGGCAGCC AGCTCAGCTC CGGAGAGCCG GCGGCGCGGC GGGCATGGCT 
CGGGTGGCGT GGGGGCTGCT GTGGTTGCTG CTGGGCAGCG CCGGGGCGCA GTACGAGAAG 
TACAGCTTCC GGGGCTTCCC GCCCGAGGAC CTGATGCCGC TGGCCGCGGC GTACGGGCAC 
GCTCTGGAGC AGTACGAGGG AGAGAGCTGG CGCGAGAGCG CGCGCTACCT GGAGGCGGCG 
CTGCGGCTGC ACCGGCTCCT GCGCGACAGC GAGGCCTTCT GCCACGCCAA CTGCAGCGGC 
CCCGCGCCCG CGGCCAAGCC CGATCCCGAC GGCGGCCGCG CAGACGAGTG GGCCTGCGAG 
CTGCGGCTCT TCGGCCGCGT CCTGGAGCGA GCCGCCTGCC TGCGGCGCTG CAAGCGGACG 
CTGCCCGCCT TCCAGGTGCC CTACCCGCCG CGGCAGCTGC TGCGTGACTT CCAGAGCCGC 
CTGCCCTACC AGTACCTGCA CTACGCGCTG TTCAAGGCTA ACCGGCTGGA GAAGGCGGTG 
GCGGCGGCCT ACACCTTCCT CCAGAGGAAC CCGAAGCACG AGCTGACCGC CAAGTATCTC 
AACTACTATC AGGGGATGCT GGACGTCGCC GACGAGTCCC TCACGGACCT AGAGGCCCAG 
CCCTACGAGG CCGTGTTCCT CCGGGCTGTG AAGCTCTACA ACAGCGGGGA TTTCCGCAGC 
AGCACGGAGG ACATGGAGCG GGCCTTGTCA GAGTACCTGG CAGTCTTTGC CCGGTGCCTG 
GCCGGCTGTG AAGGGGCCCA TGAGCAGGTG GACTTCAAGG ACTTCTACCC GGCCATAGCA 
GATCTCTTTG CAGAGTCCCT GCAGTGCAAG GTGGACTGTG AGGCCAATTT GACCCCCAAT 
GTGGGTGGCT ACTTCGTGGA CAAGTTCGTG GCCACCATGT ACCACTACCT GCAGTTTGCC 
TACTATAAGT TGAATGATGT GCGCCAGGCT GCCCGCAGCG CCGCCAGCTA CATGCTCTTC 
GACCCCAAGG ACAGCGTCAT GCAGCAGAAC CTGGTGTATT ACCGGTTCCA CCGGGCTCGC 
TGGGGCCTGG AAGAGGAGGA CTTCCAGCCC CGGGAGGAGG CCATGCTCTA CCACAACCAG 
ACCGCCGAGC TGCGGGAGCT GCTGGAGTTC ACCCACATGT ACCTGCAGTC AGATGATGAG 
AGCCAGAGCC TGAACTCGCA TGAGAAGGGG ACACCCCACA CCGCTCAAGC TTGGGAAGCC 
TGGTGCCGAT GGCCCCACCC TCACCAGCCT GGGCAGCAGC AAGAACTATT TATTAAAAAC 
TTAAGATGGG CCAGGTGCGG TGGCTCACAC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006721703.1
CDS	409..2004
Translation	MGRGFLGFPSLAGSAPRRILYLGDTPKAGARADGEDLTTLSDPLGKEGRRRDPGPAPPCRLVPGAKTQRARRPPGARPCPLPSAGQPAQLRRAGGAAGMARVAWGLLWLLLGSAGAQYEKYSFRGFPPEDLMPLAAAYGHALEQYEGESWRESARYLEAALRLHRLLRDSEAFCHANCSGPAPAAKPDPDGGRADEWACELRLFGRVLERAACLRRCKRTLPAFQVPYPPRQLLRDFQSRLPYQYLHYALFKANRLEKAVAAAYTFLQRNPKHELTAKYLNYYQGMLDVADESLTDLEAQPYEAVFLRAVKLYNSGDFRSSTEDMERALSEYLAVFARCLAGCEGAHEQVDFKDFYPAIADLFAESLQCKVDCEANLTPNVGGYFVDKFVATMYHYLQFAYYKLNDVRQAARSAASYMLFDPKDSVMQQNLVYYRFHRARWGLEEEDFQPREEAMLYHNQTAELRELLEFTHMYLQSDDESQSLNSHEKGTPHTAQAWEAWCRWPHPHQPGQQQELFIKNLRWARCGGSHL

Target ORF information:

RefSeq Version	XM_006721640.3
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 3-hydroxylase family member 4 (non-enzymatic) (P3H4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721640.3

ORF Insert Sequence:

ATGGGGAGGG GCTTCCTGGG CTTCCCATCT CTGGCGGGAA GCGCTCCCCG ACGCATTCTC 
TACCTAGGGG ACACCCCCAA GGCAGGAGCC CGGGCCGACG GAGAGGACTT AACGACACTA 
TCGGACCCTC TGGGAAAAGA GGGGAGACGT CGTGACCCAG GCCCCGCCCC ACCTTGCCGC 
CTCGTGCCCG GCGCTAAGAC CCAGCGGGCG CGCCGCCCGC CCGGGGCCCG GCCCTGTCCC 
CTTCCGTCCG CGGGGCAGCC AGCTCAGCTC CGGAGAGCCG GCGGCGCGGC GGGCATGGCT 
CGGGTGGCGT GGGGGCTGCT GTGGTTGCTG CTGGGCAGCG CCGGGGCGCA GTACGAGAAG 
TACAGCTTCC GGGGCTTCCC GCCCGAGGAC CTGATGCCGC TGGCCGCGGC GTACGGGCAC 
GCTCTGGAGC AGTACGAGGG AGAGAGCTGG CGCGAGAGCG CGCGCTACCT GGAGGCGGCG 
CTGCGGCTGC ACCGGCTCCT GCGCGACAGC GAGGCCTTCT GCCACGCCAA CTGCAGCGGC 
CCCGCGCCCG CGGCCAAGCC CGATCCCGAC GGCGGCCGCG CAGACGAGTG GGCCTGCGAG 
CTGCGGCTCT TCGGCCGCGT CCTGGAGCGA GCCGCCTGCC TGCGGCGCTG CAAGCGGACG 
CTGCCCGCCT TCCAGGTGCC CTACCCGCCG CGGCAGCTGC TGCGTGACTT CCAGAGCCGC 
CTGCCCTACC AGTACCTGCA CTACGCGCTG TTCAAGGCTA ACCGGCTGGA GAAGGCGGTG 
GCGGCGGCCT ACACCTTCCT CCAGAGGAAC CCGAAGCACG AGCTGACCGC CAAGTATCTC 
AACTACTATC AGGGGATGCT GGACGTCGCC GACGAGTCCC TCACGGACCT AGAGGCCCAG 
CCCTACGAGG CCGTGTTCCT CCGGGCTGTG AAGCTCTACA ACAGCGGGGA TTTCCGCAGC 
AGCACGGAGG ACATGGAGCG GGCCTTGTCA GAGTACCTGG CAGTCTTTGC CCGGTGCCTG 
GCCGGCTGTG AAGGGGCCCA TGAGCAGGTG GACTTCAAGG ACTTCTACCC GGCCATAGCA 
GATCTCTTTG CAGAGTCCCT GCAGTGCAAG GTGGACTGTG AGGCCAATTT GACCCCCAAT 
GTGGGTGGCT ACTTCGTGGA CAAGTTCGTG GCCACCATGT ACCACTACCT GCAGTTTGCC 
TACTATAAGT TGAATGATGT GCGCCAGGCT GCCCGCAGCG CCGCCAGCTA CATGCTCTTC 
GACCCCAAGG ACAGCGTCAT GCAGCAGAAC CTGGTGTATT ACCGGTTCCA CCGGGCTCGC 
TGGGGCCTGG AAGAGGAGGA CTTCCAGCCC CGGGAGGAGG CCATGCTCTA CCACAACCAG 
ACCGCCGAGC TGCGGGAGCT GCTGGAGTTC ACCCACATGT ACCTGCAGTC AGATGATGAG 
AGCCAGAGCC TGAACTCGCA TGAGAAGGGG ACACCCCACA CCGCTCAAGC TTGGGAAGCC 
TGGTGCCGAT GGCCCCACCC TCACCAGCCT GGGCAGCAGC AAGAACTATT TATTAAAAAC 
TTAAGATGGG CCAGGTGCGG TGGCTCACAC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3H4 ( XM_006721640.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006721703.1 Homo sapiens prolyl 3-hydroxylase family member 4 (inactive) (P3H4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu50033
Clone ID Related Accession (Same CDS sequence)	XM_006721640.3 , XM_006721640.4
Accession Version	XM_006721640.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoplasmic reticulum protein SC65 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006721640.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGGG GCTTCCTGGG CTTCCCATCT CTGGCGGGAA GCGCTCCCCG ACGCATTCTC 
TACCTAGGGG ACACCCCCAA GGCAGGAGCC CGGGCCGACG GAGAGGACTT AACGACACTA 
TCGGACCCTC TGGGAAAAGA GGGGAGACGT CGTGACCCAG GCCCCGCCCC ACCTTGCCGC 
CTCGTGCCCG GCGCTAAGAC CCAGCGGGCG CGCCGCCCGC CCGGGGCCCG GCCCTGTCCC 
CTTCCGTCCG CGGGGCAGCC AGCTCAGCTC CGGAGAGCCG GCGGCGCGGC GGGCATGGCT 
CGGGTGGCGT GGGGGCTGCT GTGGTTGCTG CTGGGCAGCG CCGGGGCGCA GTACGAGAAG 
TACAGCTTCC GGGGCTTCCC GCCCGAGGAC CTGATGCCGC TGGCCGCGGC GTACGGGCAC 
GCTCTGGAGC AGTACGAGGG AGAGAGCTGG CGCGAGAGCG CGCGCTACCT GGAGGCGGCG 
CTGCGGCTGC ACCGGCTCCT GCGCGACAGC GAGGCCTTCT GCCACGCCAA CTGCAGCGGC 
CCCGCGCCCG CGGCCAAGCC CGATCCCGAC GGCGGCCGCG CAGACGAGTG GGCCTGCGAG 
CTGCGGCTCT TCGGCCGCGT CCTGGAGCGA GCCGCCTGCC TGCGGCGCTG CAAGCGGACG 
CTGCCCGCCT TCCAGGTGCC CTACCCGCCG CGGCAGCTGC TGCGTGACTT CCAGAGCCGC 
CTGCCCTACC AGTACCTGCA CTACGCGCTG TTCAAGGCTA ACCGGCTGGA GAAGGCGGTG 
GCGGCGGCCT ACACCTTCCT CCAGAGGAAC CCGAAGCACG AGCTGACCGC CAAGTATCTC 
AACTACTATC AGGGGATGCT GGACGTCGCC GACGAGTCCC TCACGGACCT AGAGGCCCAG 
CCCTACGAGG CCGTGTTCCT CCGGGCTGTG AAGCTCTACA ACAGCGGGGA TTTCCGCAGC 
AGCACGGAGG ACATGGAGCG GGCCTTGTCA GAGTACCTGG CAGTCTTTGC CCGGTGCCTG 
GCCGGCTGTG AAGGGGCCCA TGAGCAGGTG GACTTCAAGG ACTTCTACCC GGCCATAGCA 
GATCTCTTTG CAGAGTCCCT GCAGTGCAAG GTGGACTGTG AGGCCAATTT GACCCCCAAT 
GTGGGTGGCT ACTTCGTGGA CAAGTTCGTG GCCACCATGT ACCACTACCT GCAGTTTGCC 
TACTATAAGT TGAATGATGT GCGCCAGGCT GCCCGCAGCG CCGCCAGCTA CATGCTCTTC 
GACCCCAAGG ACAGCGTCAT GCAGCAGAAC CTGGTGTATT ACCGGTTCCA CCGGGCTCGC 
TGGGGCCTGG AAGAGGAGGA CTTCCAGCCC CGGGAGGAGG CCATGCTCTA CCACAACCAG 
ACCGCCGAGC TGCGGGAGCT GCTGGAGTTC ACCCACATGT ACCTGCAGTC AGATGATGAG 
AGCCAGAGCC TGAACTCGCA TGAGAAGGGG ACACCCCACA CCGCTCAAGC TTGGGAAGCC 
TGGTGCCGAT GGCCCCACCC TCACCAGCCT GGGCAGCAGC AAGAACTATT TATTAAAAAC 
TTAAGATGGG CCAGGTGCGG TGGCTCACAC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006721703.1
CDS	502..2097
Translation	MGRGFLGFPSLAGSAPRRILYLGDTPKAGARADGEDLTTLSDPLGKEGRRRDPGPAPPCRLVPGAKTQRARRPPGARPCPLPSAGQPAQLRRAGGAAGMARVAWGLLWLLLGSAGAQYEKYSFRGFPPEDLMPLAAAYGHALEQYEGESWRESARYLEAALRLHRLLRDSEAFCHANCSGPAPAAKPDPDGGRADEWACELRLFGRVLERAACLRRCKRTLPAFQVPYPPRQLLRDFQSRLPYQYLHYALFKANRLEKAVAAAYTFLQRNPKHELTAKYLNYYQGMLDVADESLTDLEAQPYEAVFLRAVKLYNSGDFRSSTEDMERALSEYLAVFARCLAGCEGAHEQVDFKDFYPAIADLFAESLQCKVDCEANLTPNVGGYFVDKFVATMYHYLQFAYYKLNDVRQAARSAASYMLFDPKDSVMQQNLVYYRFHRARWGLEEEDFQPREEAMLYHNQTAELRELLEFTHMYLQSDDESQSLNSHEKGTPHTAQAWEAWCRWPHPHQPGQQQELFIKNLRWARCGGSHL

Target ORF information:

RefSeq Version	XM_006721640.4
Organism	Homo sapiens(human)
Definition	Homo sapiens prolyl 3-hydroxylase family member 4 (inactive) (P3H4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721640.4

ORF Insert Sequence:

ATGGGGAGGG GCTTCCTGGG CTTCCCATCT CTGGCGGGAA GCGCTCCCCG ACGCATTCTC 
TACCTAGGGG ACACCCCCAA GGCAGGAGCC CGGGCCGACG GAGAGGACTT AACGACACTA 
TCGGACCCTC TGGGAAAAGA GGGGAGACGT CGTGACCCAG GCCCCGCCCC ACCTTGCCGC 
CTCGTGCCCG GCGCTAAGAC CCAGCGGGCG CGCCGCCCGC CCGGGGCCCG GCCCTGTCCC 
CTTCCGTCCG CGGGGCAGCC AGCTCAGCTC CGGAGAGCCG GCGGCGCGGC GGGCATGGCT 
CGGGTGGCGT GGGGGCTGCT GTGGTTGCTG CTGGGCAGCG CCGGGGCGCA GTACGAGAAG 
TACAGCTTCC GGGGCTTCCC GCCCGAGGAC CTGATGCCGC TGGCCGCGGC GTACGGGCAC 
GCTCTGGAGC AGTACGAGGG AGAGAGCTGG CGCGAGAGCG CGCGCTACCT GGAGGCGGCG 
CTGCGGCTGC ACCGGCTCCT GCGCGACAGC GAGGCCTTCT GCCACGCCAA CTGCAGCGGC 
CCCGCGCCCG CGGCCAAGCC CGATCCCGAC GGCGGCCGCG CAGACGAGTG GGCCTGCGAG 
CTGCGGCTCT TCGGCCGCGT CCTGGAGCGA GCCGCCTGCC TGCGGCGCTG CAAGCGGACG 
CTGCCCGCCT TCCAGGTGCC CTACCCGCCG CGGCAGCTGC TGCGTGACTT CCAGAGCCGC 
CTGCCCTACC AGTACCTGCA CTACGCGCTG TTCAAGGCTA ACCGGCTGGA GAAGGCGGTG 
GCGGCGGCCT ACACCTTCCT CCAGAGGAAC CCGAAGCACG AGCTGACCGC CAAGTATCTC 
AACTACTATC AGGGGATGCT GGACGTCGCC GACGAGTCCC TCACGGACCT AGAGGCCCAG 
CCCTACGAGG CCGTGTTCCT CCGGGCTGTG AAGCTCTACA ACAGCGGGGA TTTCCGCAGC 
AGCACGGAGG ACATGGAGCG GGCCTTGTCA GAGTACCTGG CAGTCTTTGC CCGGTGCCTG 
GCCGGCTGTG AAGGGGCCCA TGAGCAGGTG GACTTCAAGG ACTTCTACCC GGCCATAGCA 
GATCTCTTTG CAGAGTCCCT GCAGTGCAAG GTGGACTGTG AGGCCAATTT GACCCCCAAT 
GTGGGTGGCT ACTTCGTGGA CAAGTTCGTG GCCACCATGT ACCACTACCT GCAGTTTGCC 
TACTATAAGT TGAATGATGT GCGCCAGGCT GCCCGCAGCG CCGCCAGCTA CATGCTCTTC 
GACCCCAAGG ACAGCGTCAT GCAGCAGAAC CTGGTGTATT ACCGGTTCCA CCGGGCTCGC 
TGGGGCCTGG AAGAGGAGGA CTTCCAGCCC CGGGAGGAGG CCATGCTCTA CCACAACCAG 
ACCGCCGAGC TGCGGGAGCT GCTGGAGTTC ACCCACATGT ACCTGCAGTC AGATGATGAG 
AGCCAGAGCC TGAACTCGCA TGAGAAGGGG ACACCCCACA CCGCTCAAGC TTGGGAAGCC 
TGGTGCCGAT GGCCCCACCC TCACCAGCCT GGGCAGCAGC AAGAACTATT TATTAAAAAC 
TTAAGATGGG CCAGGTGCGG TGGCTCACAC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA.
The Journal of biological chemistry292(9)3877-3887(2017 03)
Hudson DM,Weis M,Rai J,Joeng KS,Dimori M,Lee BH,Morello R,Eyre DR

Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research29(3)666-75(2014 Mar)
Gruenwald K,Castagnola P,Besio R,Dimori M,Chen Y,Akel NS,Swain FL,Skinner RA,Eyre DR,Gaddy D,Suva LJ,Morello R

Identification and characterization of a new autoimmune protein in membranous nephropathy by immunoscreening of a renal cDNA library.
PloS one7(11)e48845(2012)
Cavazzini F,Magistroni R,Furci L,Lupo V,Ligabue G,Granito M,Leonelli M,Albertazzi A,Cappelli G

Insulin-dependent interactions of proteins with GLUT4 revealed through stable isotope labeling by amino acids in cell culture (SILAC).
Journal of proteome research5(1)64-75(2006 Jan)
Foster LJ,Rudich A,Talior I,Patel N,Huang X,Furtado LM,Bilan PJ,Mann M,Klip A

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P3H4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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