tirA cDNA ORF clone, Heterostelium album PN500

Gene
Clones
gRNAs

Summary

Gene Symbol	tirA
Entrez Gene ID	31358495
Full Name	Toll-Interleukin receptor domain-containing protein
Gene Type	protein-coding
Organism	Heterostelium album PN500

mRNA and Protein(s)

mRNA	Protein	Name
XM_020573947.1	XP_020436019.1	Toll-Interleukin receptor domain-containing protein

Pathways from BioSystems
Gene Ontology

The following tirA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tirA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo219869	XM_020573947.1 Latest version!	Polysphondylium pallidum PN500 Toll-Interleukin receptor domain-containing protein (tirA), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tirA ( XM_020573947.1 ) cDNA ORF clone, Heterostelium album PN500 -> XP_020436019.1 Polysphondylium pallidum PN500 Toll-Interleukin receptor domain-containing protein (tirA), partial mRNA.

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CloneID	OPo219869
Clone ID Related Accession (Same CDS sequence)	XM_020573947.1
Accession Version	XM_020573947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-03-16
Organism	Heterostelium album PN500
Product	Toll-Interleukin receptor domain-containing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_008805065). COMPLETENESS: incomplete on both ends.

ATGCCTATAT CTAGTAGTAA TAATAGTGGT GGTGGTAACA CGACAACATC ATCATCAGTA 
TCAAAAAAAT TGGTATTTAA AAATAAAAAG ATATTCTTAA TACCAAAAAC ATCGAGTGTT 
TCATGGTCAC ATAAAGCCCA TGCCAAGAAA ACCTTGGAGA TATATGGTGC AACCGTTAGT 
TTCGCCATAG TACCAAAAGA AACTGATTAT ATCGTAGTTC TAGAGGGTCA TGATAGATCG 
AGAACCTCTT TACATCCAAA TCTGATGTCA GCATATCAAA AATCAATCAA GTTCAATATT 
CCAATGTTTA TTGAGGATTT TATTTATTCA TCTATATCAT CTGCCAAACT GAAAGATTTA 
CCAAGTTTTA GATTCAACAT GGAATTACCA AAGAATAGTA TCAACGGCAA TAGTAAATAT 
TTTGATTTAG GATATGATGA TGATGATACA AATAGTGAAA GTAGTTCTAA GGATGTCTAT 
GGATTGTTCG ATGATTTCGA AACAACGAAT AATACACAAA CAGCAGAAAC TTTGGATAGT 
GATGACGATG ATGAATCAAT TTATAGTGAA GCTGATATGT TTGGGTTATT TGACGATTAC 
ATAGCCCCAA AGACATCAAA ACAACAACAA CCAAATCAAC AACAACAACA ACAAATAGCA 
TATTTTGATG ATGAAGAGGA GAACGACTTT TTTGCGATGT TTGGGGATTC ACCAATCATT 
TCCCAACAAC CCCAATTAAC TTTTGAAGAT GAAGAAGAAG AAGAATCAAT TCAAGTTACT 
ATTCCCAAAA ATAAAATACA ACCTCAACTT CAACCTCAGC TTCAACCTCA ACCTCAAATC 
CAATCTAAAA CTCAATTGAT ATTTAATGAA GAAAATGAAA TTAAAGATAT GTTTGGTATG 
GATACTCCAT ATTTCAATAC AAAAAATTCA AATGTAGGAT ATTCATTTTA TGTTGTTGGT 
GATAAATTAA AGTTGATAGA GTTACCTTTA AAACTGAAAC AGATTGATGT GTCGCAGAGT 
GGATTCATTT CTTTTATCGA TTGCACTGAC AAACTGTATA GTTGGGGAAA GCTTTCAAAC 
TCTGTTAAAT TCCCACAAGT TAAAAAGCTG ACTGCTCCTC AGAATGTTAC AAAATTCCTT 
GATGTCATTT GTACTAAAAA TAATATCATT TCATTGGATA CTGAAGGTCA ATGTTGGGAA 
ATAGAGGAGA AAAAACAACA ACAGCCACAA CAACAAGAAC AAAATCAAAA ACAACAAAGA 
CAGAAACTAT CACGTGGAGT TGGTGTTGCT GTTCAAGATA ATCCTGTTGA TTTCAGTTTG 
AAACATTTGA AACTTCCATT TTTGGTAAAG ACAATATCGA GTGGAAATCA ATTTGTTGTG 
GCACTAACCG AGAGTGGAAG ACTTGTATCG TGGGGAACAA CCAATAATTT GAGAGGACAA 
TTGGGTAGAG AGTTGAAGAA TCCAAAGGAT TCAAACATTC CACAGTTCAT TCCAGATGTT 
CAGAGAGCAA CTTTCGTAAG TTGTGGATTT GAACATAGCA TGGCTATTGT CATACAAAAT 
GGAGTGGAGA GTGTCTTTGG TTGGGGAGAC AGCGCTAAAC AACAACTTGG TTACATAAGT 
GAGAAGGCGC AAAACACAGC AACCCCGATG CTCATTCATA CCAAACAAAC TCATATCAAT 
GTGATATGCA ATGAGAACTA CTCTGTTATC ATTGATGAAG ATATTAAATT CTCTGGATTA 
TCATTCCCTA TGGAATTCCA TACCATTAAT CTAGTTATTC ATCCATTGAG AACAATACCA 
AATACTTTCA AATTGTTCAA ATACAAACAT TCGGTTGGAG TTACACTCTT TGATACCTAT 
TCAACAGCAT ATTGTTTCAT TCAAGTTGAT CGATTAGACC TATTAAAGTC GCTTCTCTCT 
GGAAAATTGG ATTTCAGCCA AACTAAAATG ATCTCATTAA TAATGTATTC CATTGAATTG 
AATAATTTAG AAATATTCAA ATATCTCTAT CATAGATTTG AAAATGTTTC ATTAGAAGTT 
AGAACAGGAA ATGCAGATCT TTCTTATCTA CATTATGCGG TGGAGAACAT CTGTTTACCA 
ATTATGAAAT ACATCTTAAA GAACAAACTT CTCAAAGTTG ATATTACAAA TAGTGTTGGT 
GATACTCCAC TTCATACTTC TTCTAATGAA TCATTATTGA TGTTATGTGA ATATGGTTGT 
GATAGAAATC TAAAGAATGC TAAAGGATAT ACTGCATTAC ATCTTGCTAC TCAAGATAAT 
AAGCTAGCAA TATCGATGTT GTTGTTAAAG AATGGTGCCC AAGCAATCAC TGATCGTGCA 
ATGAAAACAC CGTTACAGTA TGTCAATGGT AAAGAGAAAA CCTTGCTCCA GAACATTATG 
TTTACCAATG AAGTATTCCT ATCGTATGCA CACAAGGATA TCGGATTTGT TAGAGAACTT 
CGTATTGCAA TGGAACAATA CTCAATTAGA TGTTGGCTAG ACGAGTATAG ATTACAAGCC 
GGTTGTGACT GGAGAGCTGA AATCACCAAA GGTGTAGCCA ATGCTCAAGT TATCATCTAT 
ACCATATCAG AAACATCTTC TATTTCTCTT TGGTGTAGAA AGGAGTTGAA AATGGCAAGA 
AGATTAGGTA AAACTATTAT TGCTCTCTAT TATCATAATG TCGAAATAGA TCCAATCTTG 
AGAGGTTTGT TCGACTATGA ATTTAAAACA GCTGCACCTC TTGACCCAAA TGAAGACATC 
TTCATCACAG AGATGGAAAA ACTAGCAAAG ATCACCAAGA ATATTATTCA TGGTCATAAG 
CTAGCAAAGA TATATCCAAC TCAAAAGGAT TTGATTGAAC ACTTTGGAAA TAGATCAATT 
TATATCAGTT TCTGTGATAG AGAGTACAAG TTGTTCAATT TCATTAGAGA ACTATTGTTG 
ATTAGAGGTG TTCCTATCTT CGATCGTGAT TTTCTTTTAG GTAATACGAA CAAGAATGTT 
GCCAAGGTTG TCAATACAGC GGTCGAAGCA AAAGAAGAGA AAGAACAGAC CATTAGGCTC 
ACAAAGCGAG AGAGAAGATT ACATTCAGTG TTTGGATTCA GTCAAGATCT CTTTGACAGC 
CCACAACAAG AAGTACCAAT TCTCAACGAC TATACAATGA CTCTGGCCCA CCAATCAGAA 
CATCCGGATG ATCAAGCTGA TCAGAAATCA CCAGTGGAGA AAATTAACAA ATCATCAGAT 
GAATATCTTG AGAAATTCAA GGACATCCAG GATCTGCACC CCCACATCTC AAAGAGCATT 
CTACATCTTG TAATCAATCC ATCGGTCGAC TCTGAATTCT CACAAATTCA AAAAGAGATT 
GAATTCTCAA CTGGTGATGA AAAGAGTATC TTTGTAATTA CAACAAACTA CAAAGAGAAA 
TCTAAATGTA ACAATATGCT GAAGAACTGT ACTTGGTTTG AGCTAAACTA TCAGAAGGAA 
GATGAATTCA TAGATAAGAT TTTATTTAAC TATGAAGTAA TTGATAAGAC TCATCTAACC 
ATCAACAGAA TCATGCATGA ACTCTCCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020436019.1
CDS	1..3573
Translation	MPISSSNNSGGGNTTTSSSVSKKLVFKNKKIFLIPKTSSVSWSHKAHAKKTLEIYGATVSFAIVPKETDYIVVLEGHDRSRTSLHPNLMSAYQKSIKFNIPMFIEDFIYSSISSAKLKDLPSFRFNMELPKNSINGNSKYFDLGYDDDDTNSESSSKDVYGLFDDFETTNNTQTAETLDSDDDDESIYSEADMFGLFDDYIAPKTSKQQQPNQQQQQQIAYFDDEEENDFFAMFGDSPIISQQPQLTFEDEEEEESIQVTIPKNKIQPQLQPQLQPQPQIQSKTQLIFNEENEIKDMFGMDTPYFNTKNSNVGYSFYVVGDKLKLIELPLKLKQIDVSQSGFISFIDCTDKLYSWGKLSNSVKFPQVKKLTAPQNVTKFLDVICTKNNIISLDTEGQCWEIEEKKQQQPQQQEQNQKQQRQKLSRGVGVAVQDNPVDFSLKHLKLPFLVKTISSGNQFVVALTESGRLVSWGTTNNLRGQLGRELKNPKDSNIPQFIPDVQRATFVSCGFEHSMAIVIQNGVESVFGWGDSAKQQLGYISEKAQNTATPMLIHTKQTHINVICNENYSVIIDEDIKFSGLSFPMEFHTINLVIHPLRTIPNTFKLFKYKHSVGVTLFDTYSTAYCFIQVDRLDLLKSLLSGKLDFSQTKMISLIMYSIELNNLEIFKYLYHRFENVSLEVRTGNADLSYLHYAVENICLPIMKYILKNKLLKVDITNSVGDTPLHTSSNESLLMLCEYGCDRNLKNAKGYTALHLATQDNKLAISMLLLKNGAQAITDRAMKTPLQYVNGKEKTLLQNIMFTNEVFLSYAHKDIGFVRELRIAMEQYSIRCWLDEYRLQAGCDWRAEITKGVANAQVIIYTISETSSISLWCRKELKMARRLGKTIIALYYHNVEIDPILRGLFDYEFKTAAPLDPNEDIFITEMEKLAKITKNIIHGHKLAKIYPTQKDLIEHFGNRSIYISFCDREYKLFNFIRELLLIRGVPIFDRDFLLGNTNKNVAKVVNTAVEAKEEKEQTIRLTKRERRLHSVFGFSQDLFDSPQQEVPILNDYTMTLAHQSEHPDDQADQKSPVEKINKSSDEYLEKFKDIQDLHPHISKSILHLVINPSVDSEFSQIQKEIEFSTGDEKSIFVITTNYKEKSKCNNMLKNCTWFELNYQKEDEFIDKILFNYEVIDKTHLTINRIMHELSK

Target ORF information:

RefSeq Version	XM_020573947.1
Organism	Heterostelium album PN500
Definition	Polysphondylium pallidum PN500 Toll-Interleukin receptor domain-containing protein (tirA), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020573947.1

ORF Insert Sequence:

ATGCCTATAT CTAGTAGTAA TAATAGTGGT GGTGGTAACA CGACAACATC ATCATCAGTA 
TCAAAAAAAT TGGTATTTAA AAATAAAAAG ATATTCTTAA TACCAAAAAC ATCGAGTGTT 
TCATGGTCAC ATAAAGCCCA TGCCAAGAAA ACCTTGGAGA TATATGGTGC AACCGTTAGT 
TTCGCCATAG TACCAAAAGA AACTGATTAT ATCGTAGTTC TAGAGGGTCA TGATAGATCG 
AGAACCTCTT TACATCCAAA TCTGATGTCA GCATATCAAA AATCAATCAA GTTCAATATT 
CCAATGTTTA TTGAGGATTT TATTTATTCA TCTATATCAT CTGCCAAACT GAAAGATTTA 
CCAAGTTTTA GATTCAACAT GGAATTACCA AAGAATAGTA TCAACGGCAA TAGTAAATAT 
TTTGATTTAG GATATGATGA TGATGATACA AATAGTGAAA GTAGTTCTAA GGATGTCTAT 
GGATTGTTCG ATGATTTCGA AACAACGAAT AATACACAAA CAGCAGAAAC TTTGGATAGT 
GATGACGATG ATGAATCAAT TTATAGTGAA GCTGATATGT TTGGGTTATT TGACGATTAC 
ATAGCCCCAA AGACATCAAA ACAACAACAA CCAAATCAAC AACAACAACA ACAAATAGCA 
TATTTTGATG ATGAAGAGGA GAACGACTTT TTTGCGATGT TTGGGGATTC ACCAATCATT 
TCCCAACAAC CCCAATTAAC TTTTGAAGAT GAAGAAGAAG AAGAATCAAT TCAAGTTACT 
ATTCCCAAAA ATAAAATACA ACCTCAACTT CAACCTCAGC TTCAACCTCA ACCTCAAATC 
CAATCTAAAA CTCAATTGAT ATTTAATGAA GAAAATGAAA TTAAAGATAT GTTTGGTATG 
GATACTCCAT ATTTCAATAC AAAAAATTCA AATGTAGGAT ATTCATTTTA TGTTGTTGGT 
GATAAATTAA AGTTGATAGA GTTACCTTTA AAACTGAAAC AGATTGATGT GTCGCAGAGT 
GGATTCATTT CTTTTATCGA TTGCACTGAC AAACTGTATA GTTGGGGAAA GCTTTCAAAC 
TCTGTTAAAT TCCCACAAGT TAAAAAGCTG ACTGCTCCTC AGAATGTTAC AAAATTCCTT 
GATGTCATTT GTACTAAAAA TAATATCATT TCATTGGATA CTGAAGGTCA ATGTTGGGAA 
ATAGAGGAGA AAAAACAACA ACAGCCACAA CAACAAGAAC AAAATCAAAA ACAACAAAGA 
CAGAAACTAT CACGTGGAGT TGGTGTTGCT GTTCAAGATA ATCCTGTTGA TTTCAGTTTG 
AAACATTTGA AACTTCCATT TTTGGTAAAG ACAATATCGA GTGGAAATCA ATTTGTTGTG 
GCACTAACCG AGAGTGGAAG ACTTGTATCG TGGGGAACAA CCAATAATTT GAGAGGACAA 
TTGGGTAGAG AGTTGAAGAA TCCAAAGGAT TCAAACATTC CACAGTTCAT TCCAGATGTT 
CAGAGAGCAA CTTTCGTAAG TTGTGGATTT GAACATAGCA TGGCTATTGT CATACAAAAT 
GGAGTGGAGA GTGTCTTTGG TTGGGGAGAC AGCGCTAAAC AACAACTTGG TTACATAAGT 
GAGAAGGCGC AAAACACAGC AACCCCGATG CTCATTCATA CCAAACAAAC TCATATCAAT 
GTGATATGCA ATGAGAACTA CTCTGTTATC ATTGATGAAG ATATTAAATT CTCTGGATTA 
TCATTCCCTA TGGAATTCCA TACCATTAAT CTAGTTATTC ATCCATTGAG AACAATACCA 
AATACTTTCA AATTGTTCAA ATACAAACAT TCGGTTGGAG TTACACTCTT TGATACCTAT 
TCAACAGCAT ATTGTTTCAT TCAAGTTGAT CGATTAGACC TATTAAAGTC GCTTCTCTCT 
GGAAAATTGG ATTTCAGCCA AACTAAAATG ATCTCATTAA TAATGTATTC CATTGAATTG 
AATAATTTAG AAATATTCAA ATATCTCTAT CATAGATTTG AAAATGTTTC ATTAGAAGTT 
AGAACAGGAA ATGCAGATCT TTCTTATCTA CATTATGCGG TGGAGAACAT CTGTTTACCA 
ATTATGAAAT ACATCTTAAA GAACAAACTT CTCAAAGTTG ATATTACAAA TAGTGTTGGT 
GATACTCCAC TTCATACTTC TTCTAATGAA TCATTATTGA TGTTATGTGA ATATGGTTGT 
GATAGAAATC TAAAGAATGC TAAAGGATAT ACTGCATTAC ATCTTGCTAC TCAAGATAAT 
AAGCTAGCAA TATCGATGTT GTTGTTAAAG AATGGTGCCC AAGCAATCAC TGATCGTGCA 
ATGAAAACAC CGTTACAGTA TGTCAATGGT AAAGAGAAAA CCTTGCTCCA GAACATTATG 
TTTACCAATG AAGTATTCCT ATCGTATGCA CACAAGGATA TCGGATTTGT TAGAGAACTT 
CGTATTGCAA TGGAACAATA CTCAATTAGA TGTTGGCTAG ACGAGTATAG ATTACAAGCC 
GGTTGTGACT GGAGAGCTGA AATCACCAAA GGTGTAGCCA ATGCTCAAGT TATCATCTAT 
ACCATATCAG AAACATCTTC TATTTCTCTT TGGTGTAGAA AGGAGTTGAA AATGGCAAGA 
AGATTAGGTA AAACTATTAT TGCTCTCTAT TATCATAATG TCGAAATAGA TCCAATCTTG 
AGAGGTTTGT TCGACTATGA ATTTAAAACA GCTGCACCTC TTGACCCAAA TGAAGACATC 
TTCATCACAG AGATGGAAAA ACTAGCAAAG ATCACCAAGA ATATTATTCA TGGTCATAAG 
CTAGCAAAGA TATATCCAAC TCAAAAGGAT TTGATTGAAC ACTTTGGAAA TAGATCAATT 
TATATCAGTT TCTGTGATAG AGAGTACAAG TTGTTCAATT TCATTAGAGA ACTATTGTTG 
ATTAGAGGTG TTCCTATCTT CGATCGTGAT TTTCTTTTAG GTAATACGAA CAAGAATGTT 
GCCAAGGTTG TCAATACAGC GGTCGAAGCA AAAGAAGAGA AAGAACAGAC CATTAGGCTC 
ACAAAGCGAG AGAGAAGATT ACATTCAGTG TTTGGATTCA GTCAAGATCT CTTTGACAGC 
CCACAACAAG AAGTACCAAT TCTCAACGAC TATACAATGA CTCTGGCCCA CCAATCAGAA 
CATCCGGATG ATCAAGCTGA TCAGAAATCA CCAGTGGAGA AAATTAACAA ATCATCAGAT 
GAATATCTTG AGAAATTCAA GGACATCCAG GATCTGCACC CCCACATCTC AAAGAGCATT 
CTACATCTTG TAATCAATCC ATCGGTCGAC TCTGAATTCT CACAAATTCA AAAAGAGATT 
GAATTCTCAA CTGGTGATGA AAAGAGTATC TTTGTAATTA CAACAAACTA CAAAGAGAAA 
TCTAAATGTA ACAATATGCT GAAGAACTGT ACTTGGTTTG AGCTAAACTA TCAGAAGGAA 
GATGAATTCA TAGATAAGAT TTTATTTAAC TATGAAGTAA TTGATAAGAC TCATCTAACC 
ATCAACAGAA TCATGCATGA ACTCTCCAAA TAA