Wrn cDNA ORF clone, Heterocephalus glaber(naked mole-rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wrn
Entrez Gene ID	101724469
Full Name	Werner syndrome RecQ like helicase
Gene Type	protein-coding
Organism	Heterocephalus glaber(naked mole-rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021236848.1	XP_021092507.1	Werner syndrome ATP-dependent helicase isoform X6
XM_021236847.1	XP_021092506.1	Werner syndrome ATP-dependent helicase isoform X5
XM_013076106.1	XP_012931560.1	Werner syndrome ATP-dependent helicase
XM_021236845.1	XP_021092504.1	Werner syndrome ATP-dependent helicase isoform X2
XM_013076106.2	XP_012931560.1	Werner syndrome ATP-dependent helicase isoform X3
XM_021236843.1	XP_021092502.1	Werner syndrome ATP-dependent helicase isoform X1
XM_021236844.1	XP_021092503.1	Werner syndrome ATP-dependent helicase isoform X1
XM_021236846.1	XP_021092505.1	Werner syndrome ATP-dependent helicase isoform X4

Pathways from BioSystems
Gene Ontology

The following Wrn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wrn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHe236475	XM_021236843.1 Latest version!	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OHe236475	XM_021236844.1 Latest version!	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OHe236476	XM_021236845.1 Latest version!	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OHe236477	XM_021236846.1 Latest version!	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OHe236478	XM_021236847.1 Latest version!	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OHe236479	XM_021236848.1 Latest version!	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OHe48871	XM_013076106.2 Latest version!	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
View Old Accession Versions >>
OHe48871	XM_013076106.1	Heterocephalus glaber Werner syndrome, RecQ helicase-like (Wrn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wrn ( XM_021236843.1 ) cDNA ORF clone, Heterocephalus glaber(naked mole-rat) -> XP_021092502.1 Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHe236475
Clone ID Related Accession (Same CDS sequence)	XM_021236843.1 , XM_021236844.1
Accession Version	XM_021236843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-22
Organism	Heterocephalus glaber(naked mole-rat)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004624780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Heterocephalus glaber Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATG TAGGCACTAA TACCAAGGCT 
GATGAAAAAC ATCTTGATAA TTTTTTACCT AAAGACAAAG GTCACAGAAA TTCATTAAAC 
CTAGCTTCTT GGAATCAGCC ACTTGATGAT CCAGAGCTAG AGGATTTACT TTCAGATTCT 
CGTTCACAAA TGTCACCTGC AACCTCAAAG AGAAACTTAC CTGAGTGGTT TACCAAAGGA 
AATGAGACCA TTGTCGATAC AAGCAAGAAA CCAGTGGCCA AAACAAAAAA GACAGGTCTT 
TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021092502.1
CDS	228..4556
Translation	MNEKKLETISQQRKFPEWMSMQNKGCAIEGKKAHIQKSVFEDDLPFLEFTGSIVYSYEASDCSFLSEDISMSLSAGDVIGFDMEWPPIYNKGKLSRVALVQLCVSESKCYLFHISSMSVFPQGLKMLLENETIKKAGVGIEGDQWKLLRDFDIKLKSFVELTDVANQKLKCMEIWSLNGLVKHLFGKQLLKDKSIRCSNWSIFPLSEDQKLYAATDAYAGLIIYQKLESLGDAIQMFDTNKVEEILPSEMKKQLTAISEEVMDLANHFPDTCRKLENPQRVSIILKNISENLYSLRKMMFASTNTEPELRPRSNFKLSSLEDSTAGGQQQKQTREHKIFTRVKDETWETTLDKFIKHDGEGVLGNKMKQEGDGSEDGIKDNKLKENMERTCLMSLDNTEYELQILEQQAQEKNLSDVSFQTAERLSPSDNEKDSSYIIESDEDLEMEMLKSLENLNSDAIETIHSECSEIRGNLSLPTEEDEDKNEAFEEEEDHFLPEPNAKQVICLKTYFGHHSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLEMSNIPACFLGSAQSKNVLEDVKLGKYRIIYITPEFCSGNLDLLQHLEANIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKTALPLVPIVALTATASSSIQEDIIHCLKLSNPQITCTSFDRPNLYLEVGRKTGNILQDLQPFLVKKTSSEWEFEGPTIIYCSSRKMTEKVTAELRKLNLACGTYHAGMSFNTRKDVHHRFMRDEIQCVIATIAFGMGINKSDIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADFNLNRHHFIEIHNEKFRSYKLKMVAKMEKYLHSSRCRRQIILCHFEDRQLQKASLDIMGTENCCDNCRSRLNHCHSINDSSDTSQDFGPQAFLLLSAVDILQGKFGIGVPILFLRGSSSQRLPGPFRRHNLFGAGKDQTESWWKAFSRHLVTEGFLVDVPGGNKFVKICTLTAKGRNWLFKANAESPQRLILQANEELWPKKFLLPSASTVSLGIEHHPSNQIPIELTAEKESNLEKMYSYKALDKISSGINIPKKSIMVSSEPVISAQEQKTQSVLYGKLVEARQRHANKMDVPPAILATNKILLDLAKMRPTTVENVKQIDGVSEGKATMLAPLLEVIKHFCQANSVQADLFSNTKSQEQKKSLEMKNENCSLPKCVAVTYSLFQEKKMSLDSIAKDRILPLTAVGMHLAQAVKAGYSLDMERAGLTPEIQKIIADVIRNPPINSDMSKIKLIRMLVPENIDTYLIHMVIEILETDHDNRLLCQPSSDSSIKRCFPNSEESCSSSKRSKEDVGTNTKADEKHLDNFLPKDKGHRNSLNLASWNQPLDDPELEDLLSDSRSQMSPATSKRNLPEWFTKGNETIVDTSKKPVAKTKKTGLFS

Target ORF information:

RefSeq Version	XM_021236843.1
Organism	Heterocephalus glaber(naked mole-rat)
Definition	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021236843.1

ORF Insert Sequence:

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATG TAGGCACTAA TACCAAGGCT 
GATGAAAAAC ATCTTGATAA TTTTTTACCT AAAGACAAAG GTCACAGAAA TTCATTAAAC 
CTAGCTTCTT GGAATCAGCC ACTTGATGAT CCAGAGCTAG AGGATTTACT TTCAGATTCT 
CGTTCACAAA TGTCACCTGC AACCTCAAAG AGAAACTTAC CTGAGTGGTT TACCAAAGGA 
AATGAGACCA TTGTCGATAC AAGCAAGAAA CCAGTGGCCA AAACAAAAAA GACAGGTCTT 
TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wrn ( XM_021236844.1 ) cDNA ORF clone, Heterocephalus glaber(naked mole-rat) -> XP_021092503.1 Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHe236475
Clone ID Related Accession (Same CDS sequence)	XM_021236843.1 , XM_021236844.1
Accession Version	XM_021236844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-22
Organism	Heterocephalus glaber(naked mole-rat)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004624780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Heterocephalus glaber Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATG TAGGCACTAA TACCAAGGCT 
GATGAAAAAC ATCTTGATAA TTTTTTACCT AAAGACAAAG GTCACAGAAA TTCATTAAAC 
CTAGCTTCTT GGAATCAGCC ACTTGATGAT CCAGAGCTAG AGGATTTACT TTCAGATTCT 
CGTTCACAAA TGTCACCTGC AACCTCAAAG AGAAACTTAC CTGAGTGGTT TACCAAAGGA 
AATGAGACCA TTGTCGATAC AAGCAAGAAA CCAGTGGCCA AAACAAAAAA GACAGGTCTT 
TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021092503.1
CDS	401..4729
Translation	MNEKKLETISQQRKFPEWMSMQNKGCAIEGKKAHIQKSVFEDDLPFLEFTGSIVYSYEASDCSFLSEDISMSLSAGDVIGFDMEWPPIYNKGKLSRVALVQLCVSESKCYLFHISSMSVFPQGLKMLLENETIKKAGVGIEGDQWKLLRDFDIKLKSFVELTDVANQKLKCMEIWSLNGLVKHLFGKQLLKDKSIRCSNWSIFPLSEDQKLYAATDAYAGLIIYQKLESLGDAIQMFDTNKVEEILPSEMKKQLTAISEEVMDLANHFPDTCRKLENPQRVSIILKNISENLYSLRKMMFASTNTEPELRPRSNFKLSSLEDSTAGGQQQKQTREHKIFTRVKDETWETTLDKFIKHDGEGVLGNKMKQEGDGSEDGIKDNKLKENMERTCLMSLDNTEYELQILEQQAQEKNLSDVSFQTAERLSPSDNEKDSSYIIESDEDLEMEMLKSLENLNSDAIETIHSECSEIRGNLSLPTEEDEDKNEAFEEEEDHFLPEPNAKQVICLKTYFGHHSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLEMSNIPACFLGSAQSKNVLEDVKLGKYRIIYITPEFCSGNLDLLQHLEANIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKTALPLVPIVALTATASSSIQEDIIHCLKLSNPQITCTSFDRPNLYLEVGRKTGNILQDLQPFLVKKTSSEWEFEGPTIIYCSSRKMTEKVTAELRKLNLACGTYHAGMSFNTRKDVHHRFMRDEIQCVIATIAFGMGINKSDIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADFNLNRHHFIEIHNEKFRSYKLKMVAKMEKYLHSSRCRRQIILCHFEDRQLQKASLDIMGTENCCDNCRSRLNHCHSINDSSDTSQDFGPQAFLLLSAVDILQGKFGIGVPILFLRGSSSQRLPGPFRRHNLFGAGKDQTESWWKAFSRHLVTEGFLVDVPGGNKFVKICTLTAKGRNWLFKANAESPQRLILQANEELWPKKFLLPSASTVSLGIEHHPSNQIPIELTAEKESNLEKMYSYKALDKISSGINIPKKSIMVSSEPVISAQEQKTQSVLYGKLVEARQRHANKMDVPPAILATNKILLDLAKMRPTTVENVKQIDGVSEGKATMLAPLLEVIKHFCQANSVQADLFSNTKSQEQKKSLEMKNENCSLPKCVAVTYSLFQEKKMSLDSIAKDRILPLTAVGMHLAQAVKAGYSLDMERAGLTPEIQKIIADVIRNPPINSDMSKIKLIRMLVPENIDTYLIHMVIEILETDHDNRLLCQPSSDSSIKRCFPNSEESCSSSKRSKEDVGTNTKADEKHLDNFLPKDKGHRNSLNLASWNQPLDDPELEDLLSDSRSQMSPATSKRNLPEWFTKGNETIVDTSKKPVAKTKKTGLFS

Target ORF information:

RefSeq Version	XM_021236844.1
Organism	Heterocephalus glaber(naked mole-rat)
Definition	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021236844.1

ORF Insert Sequence:

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATG TAGGCACTAA TACCAAGGCT 
GATGAAAAAC ATCTTGATAA TTTTTTACCT AAAGACAAAG GTCACAGAAA TTCATTAAAC 
CTAGCTTCTT GGAATCAGCC ACTTGATGAT CCAGAGCTAG AGGATTTACT TTCAGATTCT 
CGTTCACAAA TGTCACCTGC AACCTCAAAG AGAAACTTAC CTGAGTGGTT TACCAAAGGA 
AATGAGACCA TTGTCGATAC AAGCAAGAAA CCAGTGGCCA AAACAAAAAA GACAGGTCTT 
TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wrn ( XM_021236845.1 ) cDNA ORF clone, Heterocephalus glaber(naked mole-rat) -> XP_021092504.1 Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X3, mRNA.

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CloneID	OHe236476
Clone ID Related Accession (Same CDS sequence)	XM_021236845.1
Accession Version	XM_021236845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-22
Organism	Heterocephalus glaber(naked mole-rat)
Product	Werner syndrome ATP-dependent helicase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004624780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Heterocephalus glaber Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATG CTGATGAAAA ACATCTTGAT 
AATTTTTTAC CTAAAGACAA AGGTCACAGA AATTCATTAA ACCTAGCTTC TTGGAATCAG 
CCACTTGATG ATCCAGAGCT AGAGGATTTA CTTTCAGATT CTCGTTCACA AATGTCACCT 
GCAACCTCAA AGAGAAACTT ACCTGAGTGG TTTACCAAAG GAAATGAGAC CATTGTCGAT 
ACAAGCAAGA AACCAGTGGC CAAAACAAAA AAGACAGGTC TTTTTAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021092504.1
CDS	228..4538
Translation	MNEKKLETISQQRKFPEWMSMQNKGCAIEGKKAHIQKSVFEDDLPFLEFTGSIVYSYEASDCSFLSEDISMSLSAGDVIGFDMEWPPIYNKGKLSRVALVQLCVSESKCYLFHISSMSVFPQGLKMLLENETIKKAGVGIEGDQWKLLRDFDIKLKSFVELTDVANQKLKCMEIWSLNGLVKHLFGKQLLKDKSIRCSNWSIFPLSEDQKLYAATDAYAGLIIYQKLESLGDAIQMFDTNKVEEILPSEMKKQLTAISEEVMDLANHFPDTCRKLENPQRVSIILKNISENLYSLRKMMFASTNTEPELRPRSNFKLSSLEDSTAGGQQQKQTREHKIFTRVKDETWETTLDKFIKHDGEGVLGNKMKQEGDGSEDGIKDNKLKENMERTCLMSLDNTEYELQILEQQAQEKNLSDVSFQTAERLSPSDNEKDSSYIIESDEDLEMEMLKSLENLNSDAIETIHSECSEIRGNLSLPTEEDEDKNEAFEEEEDHFLPEPNAKQVICLKTYFGHHSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLEMSNIPACFLGSAQSKNVLEDVKLGKYRIIYITPEFCSGNLDLLQHLEANIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKTALPLVPIVALTATASSSIQEDIIHCLKLSNPQITCTSFDRPNLYLEVGRKTGNILQDLQPFLVKKTSSEWEFEGPTIIYCSSRKMTEKVTAELRKLNLACGTYHAGMSFNTRKDVHHRFMRDEIQCVIATIAFGMGINKSDIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADFNLNRHHFIEIHNEKFRSYKLKMVAKMEKYLHSSRCRRQIILCHFEDRQLQKASLDIMGTENCCDNCRSRLNHCHSINDSSDTSQDFGPQAFLLLSAVDILQGKFGIGVPILFLRGSSSQRLPGPFRRHNLFGAGKDQTESWWKAFSRHLVTEGFLVDVPGGNKFVKICTLTAKGRNWLFKANAESPQRLILQANEELWPKKFLLPSASTVSLGIEHHPSNQIPIELTAEKESNLEKMYSYKALDKISSGINIPKKSIMVSSEPVISAQEQKTQSVLYGKLVEARQRHANKMDVPPAILATNKILLDLAKMRPTTVENVKQIDGVSEGKATMLAPLLEVIKHFCQANSVQADLFSNTKSQEQKKSLEMKNENCSLPKCVAVTYSLFQEKKMSLDSIAKDRILPLTAVGMHLAQAVKAGYSLDMERAGLTPEIQKIIADVIRNPPINSDMSKIKLIRMLVPENIDTYLIHMVIEILETDHDNRLLCQPSSDSSIKRCFPNSEESCSSSKRSKEDADEKHLDNFLPKDKGHRNSLNLASWNQPLDDPELEDLLSDSRSQMSPATSKRNLPEWFTKGNETIVDTSKKPVAKTKKTGLFS

Target ORF information:

RefSeq Version	XM_021236845.1
Organism	Heterocephalus glaber(naked mole-rat)
Definition	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021236845.1

ORF Insert Sequence:

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATG CTGATGAAAA ACATCTTGAT 
AATTTTTTAC CTAAAGACAA AGGTCACAGA AATTCATTAA ACCTAGCTTC TTGGAATCAG 
CCACTTGATG ATCCAGAGCT AGAGGATTTA CTTTCAGATT CTCGTTCACA AATGTCACCT 
GCAACCTCAA AGAGAAACTT ACCTGAGTGG TTTACCAAAG GAAATGAGAC CATTGTCGAT 
ACAAGCAAGA AACCAGTGGC CAAAACAAAA AAGACAGGTC TTTTTAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wrn ( XM_021236846.1 ) cDNA ORF clone, Heterocephalus glaber(naked mole-rat) -> XP_021092505.1 Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X5, mRNA.

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CloneID	OHe236477
Clone ID Related Accession (Same CDS sequence)	XM_021236846.1
Accession Version	XM_021236846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-22
Organism	Heterocephalus glaber(naked mole-rat)
Product	Werner syndrome ATP-dependent helicase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004624780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Heterocephalus glaber Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATG TAGGCACTAA TACCAAGGCT 
TCTACTATAT GTTGGATCTT CTTTATATAC AGCTTCACTC ACTATTTGTC ACTTATATCT 
TCATTGTTCA TGAATAACTT TTTTTTTTGG TGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021092505.1
CDS	228..4406
Translation	MNEKKLETISQQRKFPEWMSMQNKGCAIEGKKAHIQKSVFEDDLPFLEFTGSIVYSYEASDCSFLSEDISMSLSAGDVIGFDMEWPPIYNKGKLSRVALVQLCVSESKCYLFHISSMSVFPQGLKMLLENETIKKAGVGIEGDQWKLLRDFDIKLKSFVELTDVANQKLKCMEIWSLNGLVKHLFGKQLLKDKSIRCSNWSIFPLSEDQKLYAATDAYAGLIIYQKLESLGDAIQMFDTNKVEEILPSEMKKQLTAISEEVMDLANHFPDTCRKLENPQRVSIILKNISENLYSLRKMMFASTNTEPELRPRSNFKLSSLEDSTAGGQQQKQTREHKIFTRVKDETWETTLDKFIKHDGEGVLGNKMKQEGDGSEDGIKDNKLKENMERTCLMSLDNTEYELQILEQQAQEKNLSDVSFQTAERLSPSDNEKDSSYIIESDEDLEMEMLKSLENLNSDAIETIHSECSEIRGNLSLPTEEDEDKNEAFEEEEDHFLPEPNAKQVICLKTYFGHHSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLEMSNIPACFLGSAQSKNVLEDVKLGKYRIIYITPEFCSGNLDLLQHLEANIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKTALPLVPIVALTATASSSIQEDIIHCLKLSNPQITCTSFDRPNLYLEVGRKTGNILQDLQPFLVKKTSSEWEFEGPTIIYCSSRKMTEKVTAELRKLNLACGTYHAGMSFNTRKDVHHRFMRDEIQCVIATIAFGMGINKSDIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADFNLNRHHFIEIHNEKFRSYKLKMVAKMEKYLHSSRCRRQIILCHFEDRQLQKASLDIMGTENCCDNCRSRLNHCHSINDSSDTSQDFGPQAFLLLSAVDILQGKFGIGVPILFLRGSSSQRLPGPFRRHNLFGAGKDQTESWWKAFSRHLVTEGFLVDVPGGNKFVKICTLTAKGRNWLFKANAESPQRLILQANEELWPKKFLLPSASTVSLGIEHHPSNQIPIELTAEKESNLEKMYSYKALDKISSGINIPKKSIMVSSEPVISAQEQKTQSVLYGKLVEARQRHANKMDVPPAILATNKILLDLAKMRPTTVENVKQIDGVSEGKATMLAPLLEVIKHFCQANSVQADLFSNTKSQEQKKSLEMKNENCSLPKCVAVTYSLFQEKKMSLDSIAKDRILPLTAVGMHLAQAVKAGYSLDMERAGLTPEIQKIIADVIRNPPINSDMSKIKLIRMLVPENIDTYLIHMVIEILETDHDNRLLCQPSSDSSIKRCFPNSEESCSSSKRSKEDVGTNTKASTICWIFFIYSFTHYLSLISSLFMNNFFFWWH

Target ORF information:

RefSeq Version	XM_021236846.1
Organism	Heterocephalus glaber(naked mole-rat)
Definition	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021236846.1

ORF Insert Sequence:

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATG TAGGCACTAA TACCAAGGCT 
TCTACTATAT GTTGGATCTT CTTTATATAC AGCTTCACTC ACTATTTGTC ACTTATATCT 
TCATTGTTCA TGAATAACTT TTTTTTTTGG TGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wrn ( XM_021236847.1 ) cDNA ORF clone, Heterocephalus glaber(naked mole-rat) -> XP_021092506.1 Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHe236478
Clone ID Related Accession (Same CDS sequence)	XM_021236847.1
Accession Version	XM_021236847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-22
Organism	Heterocephalus glaber(naked mole-rat)
Product	Werner syndrome ATP-dependent helicase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004624780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Heterocephalus glaber Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATA TGTCACCTGC AACCTCAAAG 
AGAAACTTAC CTGAGTGGTT TACCAAAGGA AATGAGACCA TTGTCGATAC AAGCAAGAAA 
CCAGTGGCCA AAACAAAAAA GACAGGTCTT TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021092506.1
CDS	228..4406
Translation	MNEKKLETISQQRKFPEWMSMQNKGCAIEGKKAHIQKSVFEDDLPFLEFTGSIVYSYEASDCSFLSEDISMSLSAGDVIGFDMEWPPIYNKGKLSRVALVQLCVSESKCYLFHISSMSVFPQGLKMLLENETIKKAGVGIEGDQWKLLRDFDIKLKSFVELTDVANQKLKCMEIWSLNGLVKHLFGKQLLKDKSIRCSNWSIFPLSEDQKLYAATDAYAGLIIYQKLESLGDAIQMFDTNKVEEILPSEMKKQLTAISEEVMDLANHFPDTCRKLENPQRVSIILKNISENLYSLRKMMFASTNTEPELRPRSNFKLSSLEDSTAGGQQQKQTREHKIFTRVKDETWETTLDKFIKHDGEGVLGNKMKQEGDGSEDGIKDNKLKENMERTCLMSLDNTEYELQILEQQAQEKNLSDVSFQTAERLSPSDNEKDSSYIIESDEDLEMEMLKSLENLNSDAIETIHSECSEIRGNLSLPTEEDEDKNEAFEEEEDHFLPEPNAKQVICLKTYFGHHSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLEMSNIPACFLGSAQSKNVLEDVKLGKYRIIYITPEFCSGNLDLLQHLEANIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKTALPLVPIVALTATASSSIQEDIIHCLKLSNPQITCTSFDRPNLYLEVGRKTGNILQDLQPFLVKKTSSEWEFEGPTIIYCSSRKMTEKVTAELRKLNLACGTYHAGMSFNTRKDVHHRFMRDEIQCVIATIAFGMGINKSDIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADFNLNRHHFIEIHNEKFRSYKLKMVAKMEKYLHSSRCRRQIILCHFEDRQLQKASLDIMGTENCCDNCRSRLNHCHSINDSSDTSQDFGPQAFLLLSAVDILQGKFGIGVPILFLRGSSSQRLPGPFRRHNLFGAGKDQTESWWKAFSRHLVTEGFLVDVPGGNKFVKICTLTAKGRNWLFKANAESPQRLILQANEELWPKKFLLPSASTVSLGIEHHPSNQIPIELTAEKESNLEKMYSYKALDKISSGINIPKKSIMVSSEPVISAQEQKTQSVLYGKLVEARQRHANKMDVPPAILATNKILLDLAKMRPTTVENVKQIDGVSEGKATMLAPLLEVIKHFCQANSVQADLFSNTKSQEQKKSLEMKNENCSLPKCVAVTYSLFQEKKMSLDSIAKDRILPLTAVGMHLAQAVKAGYSLDMERAGLTPEIQKIIADVIRNPPINSDMSKIKLIRMLVPENIDTYLIHMVIEILETDHDNRLLCQPSSDSSIKRCFPNSEESCSSSKRSKEDMSPATSKRNLPEWFTKGNETIVDTSKKPVAKTKKTGLFS

Target ORF information:

RefSeq Version	XM_021236847.1
Organism	Heterocephalus glaber(naked mole-rat)
Definition	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021236847.1

ORF Insert Sequence:

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATA TGTCACCTGC AACCTCAAAG 
AGAAACTTAC CTGAGTGGTT TACCAAAGGA AATGAGACCA TTGTCGATAC AAGCAAGAAA 
CCAGTGGCCA AAACAAAAAA GACAGGTCTT TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wrn ( XM_021236848.1 ) cDNA ORF clone, Heterocephalus glaber(naked mole-rat) -> XP_021092507.1 Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHe236479
Clone ID Related Accession (Same CDS sequence)	XM_021236848.1
Accession Version	XM_021236848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-22
Organism	Heterocephalus glaber(naked mole-rat)
Product	Werner syndrome ATP-dependent helicase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004624780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Heterocephalus glaber Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTGT CTGCTGGAGA TGTGATAGGA TTTGACATGG AATGGCCACC AATATACAAT 
AAAGGGAAAC TAAGCAGAGT TGCACTAGTT CAATTGTGTG TGTCTGAGAG CAAATGTTAC 
TTGTTTCACA TTTCTTCTAT GTCAGTTTTT CCCCAGGGAT TAAAAATGTT GCTTGAAAAT 
GAAACAATTA AAAAGGCAGG TGTAGGAATC GAAGGAGATC AGTGGAAACT TCTACGTGAC 
TTTGACATCA AATTGAAGAG CTTTGTGGAA TTGACTGATG TTGCCAATCA AAAGTTGAAA 
TGTATGGAGA TCTGGAGCCT CAATGGACTG GTTAAACACC TCTTTGGTAA ACAACTTCTG 
AAAGACAAGT CTATCCGCTG TAGCAATTGG AGTATTTTCC CTCTCAGTGA GGATCAGAAA 
CTGTATGCAG CCACTGATGC TTATGCTGGT CTCATCATTT ATCAAAAATT AGAAAGTTTG 
GGTGATGCCA TACAGATGTT TGATACAAAT AAAGTGGAGG AAATCCTACC TAGTGAAATG 
AAGAAACAGT TGACTGCAAT CTCTGAGGAG GTGATGGATC TGGCTAATCA TTTTCCTGAC 
ACATGCAGAA AATTGGAAAA TCCACAGAGG GTTTCTATAA TATTGAAGAA TATTTCAGAA 
AATCTATATT CACTGAGGAA GATGATGTTT GCCTCTACTA ACACTGAGCC TGAACTGAGG 
CCCAGAAGTA ATTTTAAGTT ATCATCTTTG GAGGATTCAA CTGCCGGTGG ACAACAACAG 
AAACAAACTA GAGAACATAA AATTTTTACC AGAGTTAAAG ATGAGACATG GGAGACAACA 
CTTGATAAGT TCATTAAACA TGATGGAGAA GGTGTACTTG GAAATAAAAT GAAACAAGAA 
GGTGATGGTT CTGAAGATGG AATAAAAGAC AATAAATTGA AAGAAAATAT GGAAAGAACT 
TGTTTGATGT CATTAGATAA TACAGAATAT GAACTCCAAA TTTTGGAACA GCAGGCTCAA 
GAAAAAAATC TTAGTGATGT TTCTTTTCAA ACTGCTGAGC GTTTATCTCC CAGTGATAAC 
GAAAAAGATT CATCTTATAT AATTGAAAGT GATGAAGATT TAGAAATGGA GATGCTTAAG 
TCTTTAGAAA ACCTAAATAG TGATGCTATA GAAACAATTC ATTCTGAGTG CTCAGAAATA 
AGAGGAAATT TGAGTCTTCC TACTGAAGAA GATGAAGATA AAAATGAAGC CTTTGAAGAG 
GAAGAAGACC ATTTTTTGCC AGAACCCAAT GCAAAGCAAG TTATTTGCCT CAAGACCTAT 
TTTGGCCATC ATAGTTTTAA ACCGGTTCAG TGGAAAGTTA TTCATTCTGT TTTGGAAGAA 
AGAAGAGATA ATGTTGTTGT CATGGCAACT GGATATGGAA AGAGTTTATG CTTCCAGTAC 
CCACCTGTGT ATGTAGGCAA GATTGGCCTT GTCATCTCAC CTCTTATTTC CTTGATGGAA 
GACCAAGTGC TCCAGCTTGA AATGTCCAAC ATTCCAGCTT GTTTCCTTGG ATCAGCACAA 
TCAAAAAATG TTCTAGAAGA TGTTAAATTA GGCAAATATC GAATTATTTA CATAACTCCA 
GAATTCTGTT CAGGTAACTT GGACCTACTC CAGCACCTTG AAGCTAATAT TGGTATCACA 
CTGATTGCTG TGGATGAGGC TCACTGTATT TCTGAATGGG GGCATGATTT TAGAAGTTCA 
TTCAGGACAC TGGGCTCCCT GAAGACAGCA CTCCCACTGG TTCCAATAGT TGCACTCACT 
GCTACTGCAA GTTCTTCGAT CCAGGAAGAT ATTATACATT GCTTAAAACT GAGCAATCCT 
CAGATTACCT GTACTAGTTT TGATCGACCA AATCTGTATT TAGAAGTTGG ACGAAAAACA 
GGGAATATTC TTCAAGATCT ACAGCCATTT CTTGTCAAAA AGACAAGTTC TGAATGGGAA 
TTTGAAGGTC CAACTATCAT CTATTGTTCT TCCAGAAAAA TGACAGAAAA AGTTACTGCT 
GAACTTAGGA AACTAAATTT AGCCTGTGGA ACCTACCATG CAGGCATGAG TTTTAACACA 
AGGAAAGATG TTCATCACAG GTTCATGAGG GATGAAATTC AGTGTGTCAT AGCTACTATA 
GCTTTTGGAA TGGGCATTAA TAAATCTGAC ATTCGCAAAG TCATTCATTA TGGTGCACCT 
AAGGAAATGG AATCATATTA CCAGGAAATT GGTAGAGCTG GCCGTGATGG ACTTCAAAGT 
TCTTGTCATG TACTGTGGGC TCCAGCAGAC TTTAACTTAA ATAGGCACCA CTTTATTGAG 
ATACATAATG AGAAGTTTCG ATCATACAAA TTAAAGATGG TGGCAAAAAT GGAAAAATAT 
CTTCATTCCA GCAGATGTAG GCGGCAGATC ATCTTGTGTC ATTTTGAGGA CAGACAACTA 
CAAAAGGCCT CCTTGGATAT CATGGGAACT GAAAACTGCT GTGATAATTG CAGGTCCAGA 
TTGAATCATT GCCATTCCAT TAATGATTCA AGTGATACAT CACAGGACTT TGGTCCACAA 
GCATTCCTTC TGTTATCTGC TGTGGACATC TTACAGGGAA AGTTTGGAAT TGGGGTTCCA 
ATTTTATTTC TCCGAGGATC TAGTTCTCAG CGTCTCCCAG GTCCGTTTCG TAGACACAAC 
CTTTTTGGTG CTGGCAAGGA TCAAACAGAG AGTTGGTGGA AGGCCTTTTC CCGTCACCTT 
GTAACGGAAG GATTTTTGGT AGACGTTCCT GGTGGTAACA AATTTGTAAA GATTTGCACC 
CTTACGGCAA AGGGTAGGAA TTGGCTTTTT AAAGCCAATG CAGAATCTCC TCAGAGACTT 
ATACTTCAAG CTAATGAAGA GTTGTGGCCG AAGAAGTTTC TTCTGCCAAG TGCTAGTACT 
GTATCTTTGG GCATTGAACA TCATCCCTCT AATCAAATAC CAATTGAATT AACTGCAGAA 
AAGGAGTCTA ACTTGGAGAA GATGTATTCT TACAAAGCAC TTGATAAAAT TTCTTCTGGG 
ATTAACATTC CTAAAAAAAG TATCATGGTA TCCTCAGAAC CTGTTATTTC AGCCCAGGAG 
CAAAAGACCC AGAGTGTGTT GTATGGCAAA TTAGTAGAAG CTAGACAGAG ACATGCCAAT 
AAAATGGATG TTCCTCCAGC TATTCTGGCC ACAAACAAGA TACTATTGGA TTTGGCCAAA 
ATGAGACCAA CTACAGTTGA AAATGTAAAA CAAATTGATG GTGTATCTGA AGGCAAAGCT 
ACTATGTTGG CCCCTCTGTT GGAAGTCATC AAGCATTTCT GCCAAGCAAA TAGTGTTCAG 
GCAGACCTCT TTTCAAATAC AAAGTCTCAA GAACAGAAGA AAAGTTTGGA AATGAAGAAT 
GAAAACTGCT CCCTCCCGAA GTGTGTGGCT GTCACATATT CTTTATTCCA AGAAAAGAAG 
ATGTCTTTGG ACAGCATAGC CAAGGACAGG ATTCTGCCGC TCACAGCAGT TGGCATGCAC 
TTAGCCCAAG CAGTGAAAGC TGGCTACTCC CTGGATATGG AGCGGGCAGG GCTGACTCCA 
GAGATTCAGA AGATTATTGC TGATGTTATC CGAAACCCTC CCATCAACTC AGATATGAGT 
AAAATTAAAC TAATTAGAAT GTTAGTTCCT GAAAACATCG ACACATACCT TATCCACATG 
GTAATTGAGA TTCTTGAAAC TGATCATGAC AACAGACTGT TATGCCAGCC TTCAAGTGAT 
TCCAGCATAA AGAGATGTTT CCCCAACTCT GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC 
AAGGAAGATG TAGGCACTAA TACCAAGGCT GATGAAAAAC ATCTTGATAA TTTTTTACCT 
AAAGACAAAG GTCACAGAAA TTCATTAAAC CTAGCTTCTT GGAATCAGCC ACTTGATGAT 
CCAGAGCTAG AGGATTTACT TTCAGATTCT CGTTCACAAA TGTCACCTGC AACCTCAAAG 
AGAAACTTAC CTGAGTGGTT TACCAAAGGA AATGAGACCA TTGTCGATAC AAGCAAGAAA 
CCAGTGGCCA AAACAAAAAA GACAGGTCTT TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021092507.1
CDS	110..4228
Translation	MSLSAGDVIGFDMEWPPIYNKGKLSRVALVQLCVSESKCYLFHISSMSVFPQGLKMLLENETIKKAGVGIEGDQWKLLRDFDIKLKSFVELTDVANQKLKCMEIWSLNGLVKHLFGKQLLKDKSIRCSNWSIFPLSEDQKLYAATDAYAGLIIYQKLESLGDAIQMFDTNKVEEILPSEMKKQLTAISEEVMDLANHFPDTCRKLENPQRVSIILKNISENLYSLRKMMFASTNTEPELRPRSNFKLSSLEDSTAGGQQQKQTREHKIFTRVKDETWETTLDKFIKHDGEGVLGNKMKQEGDGSEDGIKDNKLKENMERTCLMSLDNTEYELQILEQQAQEKNLSDVSFQTAERLSPSDNEKDSSYIIESDEDLEMEMLKSLENLNSDAIETIHSECSEIRGNLSLPTEEDEDKNEAFEEEEDHFLPEPNAKQVICLKTYFGHHSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLEMSNIPACFLGSAQSKNVLEDVKLGKYRIIYITPEFCSGNLDLLQHLEANIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKTALPLVPIVALTATASSSIQEDIIHCLKLSNPQITCTSFDRPNLYLEVGRKTGNILQDLQPFLVKKTSSEWEFEGPTIIYCSSRKMTEKVTAELRKLNLACGTYHAGMSFNTRKDVHHRFMRDEIQCVIATIAFGMGINKSDIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADFNLNRHHFIEIHNEKFRSYKLKMVAKMEKYLHSSRCRRQIILCHFEDRQLQKASLDIMGTENCCDNCRSRLNHCHSINDSSDTSQDFGPQAFLLLSAVDILQGKFGIGVPILFLRGSSSQRLPGPFRRHNLFGAGKDQTESWWKAFSRHLVTEGFLVDVPGGNKFVKICTLTAKGRNWLFKANAESPQRLILQANEELWPKKFLLPSASTVSLGIEHHPSNQIPIELTAEKESNLEKMYSYKALDKISSGINIPKKSIMVSSEPVISAQEQKTQSVLYGKLVEARQRHANKMDVPPAILATNKILLDLAKMRPTTVENVKQIDGVSEGKATMLAPLLEVIKHFCQANSVQADLFSNTKSQEQKKSLEMKNENCSLPKCVAVTYSLFQEKKMSLDSIAKDRILPLTAVGMHLAQAVKAGYSLDMERAGLTPEIQKIIADVIRNPPINSDMSKIKLIRMLVPENIDTYLIHMVIEILETDHDNRLLCQPSSDSSIKRCFPNSEESCSSSKRSKEDVGTNTKADEKHLDNFLPKDKGHRNSLNLASWNQPLDDPELEDLLSDSRSQMSPATSKRNLPEWFTKGNETIVDTSKKPVAKTKKTGLFS

Target ORF information:

RefSeq Version	XM_021236848.1
Organism	Heterocephalus glaber(naked mole-rat)
Definition	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021236848.1

ORF Insert Sequence:

ATGAGTCTGT CTGCTGGAGA TGTGATAGGA TTTGACATGG AATGGCCACC AATATACAAT 
AAAGGGAAAC TAAGCAGAGT TGCACTAGTT CAATTGTGTG TGTCTGAGAG CAAATGTTAC 
TTGTTTCACA TTTCTTCTAT GTCAGTTTTT CCCCAGGGAT TAAAAATGTT GCTTGAAAAT 
GAAACAATTA AAAAGGCAGG TGTAGGAATC GAAGGAGATC AGTGGAAACT TCTACGTGAC 
TTTGACATCA AATTGAAGAG CTTTGTGGAA TTGACTGATG TTGCCAATCA AAAGTTGAAA 
TGTATGGAGA TCTGGAGCCT CAATGGACTG GTTAAACACC TCTTTGGTAA ACAACTTCTG 
AAAGACAAGT CTATCCGCTG TAGCAATTGG AGTATTTTCC CTCTCAGTGA GGATCAGAAA 
CTGTATGCAG CCACTGATGC TTATGCTGGT CTCATCATTT ATCAAAAATT AGAAAGTTTG 
GGTGATGCCA TACAGATGTT TGATACAAAT AAAGTGGAGG AAATCCTACC TAGTGAAATG 
AAGAAACAGT TGACTGCAAT CTCTGAGGAG GTGATGGATC TGGCTAATCA TTTTCCTGAC 
ACATGCAGAA AATTGGAAAA TCCACAGAGG GTTTCTATAA TATTGAAGAA TATTTCAGAA 
AATCTATATT CACTGAGGAA GATGATGTTT GCCTCTACTA ACACTGAGCC TGAACTGAGG 
CCCAGAAGTA ATTTTAAGTT ATCATCTTTG GAGGATTCAA CTGCCGGTGG ACAACAACAG 
AAACAAACTA GAGAACATAA AATTTTTACC AGAGTTAAAG ATGAGACATG GGAGACAACA 
CTTGATAAGT TCATTAAACA TGATGGAGAA GGTGTACTTG GAAATAAAAT GAAACAAGAA 
GGTGATGGTT CTGAAGATGG AATAAAAGAC AATAAATTGA AAGAAAATAT GGAAAGAACT 
TGTTTGATGT CATTAGATAA TACAGAATAT GAACTCCAAA TTTTGGAACA GCAGGCTCAA 
GAAAAAAATC TTAGTGATGT TTCTTTTCAA ACTGCTGAGC GTTTATCTCC CAGTGATAAC 
GAAAAAGATT CATCTTATAT AATTGAAAGT GATGAAGATT TAGAAATGGA GATGCTTAAG 
TCTTTAGAAA ACCTAAATAG TGATGCTATA GAAACAATTC ATTCTGAGTG CTCAGAAATA 
AGAGGAAATT TGAGTCTTCC TACTGAAGAA GATGAAGATA AAAATGAAGC CTTTGAAGAG 
GAAGAAGACC ATTTTTTGCC AGAACCCAAT GCAAAGCAAG TTATTTGCCT CAAGACCTAT 
TTTGGCCATC ATAGTTTTAA ACCGGTTCAG TGGAAAGTTA TTCATTCTGT TTTGGAAGAA 
AGAAGAGATA ATGTTGTTGT CATGGCAACT GGATATGGAA AGAGTTTATG CTTCCAGTAC 
CCACCTGTGT ATGTAGGCAA GATTGGCCTT GTCATCTCAC CTCTTATTTC CTTGATGGAA 
GACCAAGTGC TCCAGCTTGA AATGTCCAAC ATTCCAGCTT GTTTCCTTGG ATCAGCACAA 
TCAAAAAATG TTCTAGAAGA TGTTAAATTA GGCAAATATC GAATTATTTA CATAACTCCA 
GAATTCTGTT CAGGTAACTT GGACCTACTC CAGCACCTTG AAGCTAATAT TGGTATCACA 
CTGATTGCTG TGGATGAGGC TCACTGTATT TCTGAATGGG GGCATGATTT TAGAAGTTCA 
TTCAGGACAC TGGGCTCCCT GAAGACAGCA CTCCCACTGG TTCCAATAGT TGCACTCACT 
GCTACTGCAA GTTCTTCGAT CCAGGAAGAT ATTATACATT GCTTAAAACT GAGCAATCCT 
CAGATTACCT GTACTAGTTT TGATCGACCA AATCTGTATT TAGAAGTTGG ACGAAAAACA 
GGGAATATTC TTCAAGATCT ACAGCCATTT CTTGTCAAAA AGACAAGTTC TGAATGGGAA 
TTTGAAGGTC CAACTATCAT CTATTGTTCT TCCAGAAAAA TGACAGAAAA AGTTACTGCT 
GAACTTAGGA AACTAAATTT AGCCTGTGGA ACCTACCATG CAGGCATGAG TTTTAACACA 
AGGAAAGATG TTCATCACAG GTTCATGAGG GATGAAATTC AGTGTGTCAT AGCTACTATA 
GCTTTTGGAA TGGGCATTAA TAAATCTGAC ATTCGCAAAG TCATTCATTA TGGTGCACCT 
AAGGAAATGG AATCATATTA CCAGGAAATT GGTAGAGCTG GCCGTGATGG ACTTCAAAGT 
TCTTGTCATG TACTGTGGGC TCCAGCAGAC TTTAACTTAA ATAGGCACCA CTTTATTGAG 
ATACATAATG AGAAGTTTCG ATCATACAAA TTAAAGATGG TGGCAAAAAT GGAAAAATAT 
CTTCATTCCA GCAGATGTAG GCGGCAGATC ATCTTGTGTC ATTTTGAGGA CAGACAACTA 
CAAAAGGCCT CCTTGGATAT CATGGGAACT GAAAACTGCT GTGATAATTG CAGGTCCAGA 
TTGAATCATT GCCATTCCAT TAATGATTCA AGTGATACAT CACAGGACTT TGGTCCACAA 
GCATTCCTTC TGTTATCTGC TGTGGACATC TTACAGGGAA AGTTTGGAAT TGGGGTTCCA 
ATTTTATTTC TCCGAGGATC TAGTTCTCAG CGTCTCCCAG GTCCGTTTCG TAGACACAAC 
CTTTTTGGTG CTGGCAAGGA TCAAACAGAG AGTTGGTGGA AGGCCTTTTC CCGTCACCTT 
GTAACGGAAG GATTTTTGGT AGACGTTCCT GGTGGTAACA AATTTGTAAA GATTTGCACC 
CTTACGGCAA AGGGTAGGAA TTGGCTTTTT AAAGCCAATG CAGAATCTCC TCAGAGACTT 
ATACTTCAAG CTAATGAAGA GTTGTGGCCG AAGAAGTTTC TTCTGCCAAG TGCTAGTACT 
GTATCTTTGG GCATTGAACA TCATCCCTCT AATCAAATAC CAATTGAATT AACTGCAGAA 
AAGGAGTCTA ACTTGGAGAA GATGTATTCT TACAAAGCAC TTGATAAAAT TTCTTCTGGG 
ATTAACATTC CTAAAAAAAG TATCATGGTA TCCTCAGAAC CTGTTATTTC AGCCCAGGAG 
CAAAAGACCC AGAGTGTGTT GTATGGCAAA TTAGTAGAAG CTAGACAGAG ACATGCCAAT 
AAAATGGATG TTCCTCCAGC TATTCTGGCC ACAAACAAGA TACTATTGGA TTTGGCCAAA 
ATGAGACCAA CTACAGTTGA AAATGTAAAA CAAATTGATG GTGTATCTGA AGGCAAAGCT 
ACTATGTTGG CCCCTCTGTT GGAAGTCATC AAGCATTTCT GCCAAGCAAA TAGTGTTCAG 
GCAGACCTCT TTTCAAATAC AAAGTCTCAA GAACAGAAGA AAAGTTTGGA AATGAAGAAT 
GAAAACTGCT CCCTCCCGAA GTGTGTGGCT GTCACATATT CTTTATTCCA AGAAAAGAAG 
ATGTCTTTGG ACAGCATAGC CAAGGACAGG ATTCTGCCGC TCACAGCAGT TGGCATGCAC 
TTAGCCCAAG CAGTGAAAGC TGGCTACTCC CTGGATATGG AGCGGGCAGG GCTGACTCCA 
GAGATTCAGA AGATTATTGC TGATGTTATC CGAAACCCTC CCATCAACTC AGATATGAGT 
AAAATTAAAC TAATTAGAAT GTTAGTTCCT GAAAACATCG ACACATACCT TATCCACATG 
GTAATTGAGA TTCTTGAAAC TGATCATGAC AACAGACTGT TATGCCAGCC TTCAAGTGAT 
TCCAGCATAA AGAGATGTTT CCCCAACTCT GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC 
AAGGAAGATG TAGGCACTAA TACCAAGGCT GATGAAAAAC ATCTTGATAA TTTTTTACCT 
AAAGACAAAG GTCACAGAAA TTCATTAAAC CTAGCTTCTT GGAATCAGCC ACTTGATGAT 
CCAGAGCTAG AGGATTTACT TTCAGATTCT CGTTCACAAA TGTCACCTGC AACCTCAAAG 
AGAAACTTAC CTGAGTGGTT TACCAAAGGA AATGAGACCA TTGTCGATAC AAGCAAGAAA 
CCAGTGGCCA AAACAAAAAA GACAGGTCTT TTTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wrn ( XM_013076106.1 ) cDNA ORF clone, Heterocephalus glaber(naked mole-rat) -> XP_012931560.1 Heterocephalus glaber Werner syndrome, RecQ helicase-like (Wrn), mRNA.

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CloneID	OHe48871
Clone ID Related Accession (Same CDS sequence)	XM_013076106.1 , XM_013076106.2
Accession Version	XM_013076106.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-01
Organism	Heterocephalus glaber(Naked mole-rat)
Product	Werner syndrome ATP-dependent helicase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004624780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Heterocephalus glaber Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATG TAGGCACTAA TACCAAGATG 
TCACCTGCAA CCTCAAAGAG AAACTTACCT GAGTGGTTTA CCAAAGGAAA TGAGACCATT 
GTCGATACAA GCAAGAAACC AGTGGCCAAA ACAAAAAAGA CAGGTCTTTT TAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012931560.1
CDS	222..4418
Translation	MNEKKLETISQQRKFPEWMSMQNKGCAIEGKKAHIQKSVFEDDLPFLEFTGSIVYSYEASDCSFLSEDISMSLSAGDVIGFDMEWPPIYNKGKLSRVALVQLCVSESKCYLFHISSMSVFPQGLKMLLENETIKKAGVGIEGDQWKLLRDFDIKLKSFVELTDVANQKLKCMEIWSLNGLVKHLFGKQLLKDKSIRCSNWSIFPLSEDQKLYAATDAYAGLIIYQKLESLGDAIQMFDTNKVEEILPSEMKKQLTAISEEVMDLANHFPDTCRKLENPQRVSIILKNISENLYSLRKMMFASTNTEPELRPRSNFKLSSLEDSTAGGQQQKQTREHKIFTRVKDETWETTLDKFIKHDGEGVLGNKMKQEGDGSEDGIKDNKLKENMERTCLMSLDNTEYELQILEQQAQEKNLSDVSFQTAERLSPSDNEKDSSYIIESDEDLEMEMLKSLENLNSDAIETIHSECSEIRGNLSLPTEEDEDKNEAFEEEEDHFLPEPNAKQVICLKTYFGHHSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLEMSNIPACFLGSAQSKNVLEDVKLGKYRIIYITPEFCSGNLDLLQHLEANIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKTALPLVPIVALTATASSSIQEDIIHCLKLSNPQITCTSFDRPNLYLEVGRKTGNILQDLQPFLVKKTSSEWEFEGPTIIYCSSRKMTEKVTAELRKLNLACGTYHAGMSFNTRKDVHHRFMRDEIQCVIATIAFGMGINKSDIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADFNLNRHHFIEIHNEKFRSYKLKMVAKMEKYLHSSRCRRQIILCHFEDRQLQKASLDIMGTENCCDNCRSRLNHCHSINDSSDTSQDFGPQAFLLLSAVDILQGKFGIGVPILFLRGSSSQRLPGPFRRHNLFGAGKDQTESWWKAFSRHLVTEGFLVDVPGGNKFVKICTLTAKGRNWLFKANAESPQRLILQANEELWPKKFLLPSASTVSLGIEHHPSNQIPIELTAEKESNLEKMYSYKALDKISSGINIPKKSIMVSSEPVISAQEQKTQSVLYGKLVEARQRHANKMDVPPAILATNKILLDLAKMRPTTVENVKQIDGVSEGKATMLAPLLEVIKHFCQANSVQADLFSNTKSQEQKKSLEMKNENCSLPKCVAVTYSLFQEKKMSLDSIAKDRILPLTAVGMHLAQAVKAGYSLDMERAGLTPEIQKIIADVIRNPPINSDMSKIKLIRMLVPENIDTYLIHMVIEILETDHDNRLLCQPSSDSSIKRCFPNSEESCSSSKRSKEDVGTNTKMSPATSKRNLPEWFTKGNETIVDTSKKPVAKTKKTGLFS

Target ORF information:

RefSeq Version	XM_013076106.1
Organism	Heterocephalus glaber(Naked mole-rat)
Definition	Heterocephalus glaber Werner syndrome, RecQ helicase-like (Wrn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013076106.1

ORF Insert Sequence:

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATG TAGGCACTAA TACCAAGATG 
TCACCTGCAA CCTCAAAGAG AAACTTACCT GAGTGGTTTA CCAAAGGAAA TGAGACCATT 
GTCGATACAA GCAAGAAACC AGTGGCCAAA ACAAAAAAGA CAGGTCTTTT TAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wrn ( XM_013076106.2 ) cDNA ORF clone, Heterocephalus glaber(naked mole-rat) -> XP_012931560.1 Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X4, mRNA.

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CloneID	OHe48871
Clone ID Related Accession (Same CDS sequence)	XM_013076106.1 , XM_013076106.2
Accession Version	XM_013076106.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-22
Organism	Heterocephalus glaber(naked mole-rat)
Product	Werner syndrome ATP-dependent helicase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004624780.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 23, 2017 this sequence version replaced XM_013076106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Heterocephalus glaber Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATG TAGGCACTAA TACCAAGATG 
TCACCTGCAA CCTCAAAGAG AAACTTACCT GAGTGGTTTA CCAAAGGAAA TGAGACCATT 
GTCGATACAA GCAAGAAACC AGTGGCCAAA ACAAAAAAGA CAGGTCTTTT TAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012931560.1
CDS	228..4424
Translation	MNEKKLETISQQRKFPEWMSMQNKGCAIEGKKAHIQKSVFEDDLPFLEFTGSIVYSYEASDCSFLSEDISMSLSAGDVIGFDMEWPPIYNKGKLSRVALVQLCVSESKCYLFHISSMSVFPQGLKMLLENETIKKAGVGIEGDQWKLLRDFDIKLKSFVELTDVANQKLKCMEIWSLNGLVKHLFGKQLLKDKSIRCSNWSIFPLSEDQKLYAATDAYAGLIIYQKLESLGDAIQMFDTNKVEEILPSEMKKQLTAISEEVMDLANHFPDTCRKLENPQRVSIILKNISENLYSLRKMMFASTNTEPELRPRSNFKLSSLEDSTAGGQQQKQTREHKIFTRVKDETWETTLDKFIKHDGEGVLGNKMKQEGDGSEDGIKDNKLKENMERTCLMSLDNTEYELQILEQQAQEKNLSDVSFQTAERLSPSDNEKDSSYIIESDEDLEMEMLKSLENLNSDAIETIHSECSEIRGNLSLPTEEDEDKNEAFEEEEDHFLPEPNAKQVICLKTYFGHHSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLEMSNIPACFLGSAQSKNVLEDVKLGKYRIIYITPEFCSGNLDLLQHLEANIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKTALPLVPIVALTATASSSIQEDIIHCLKLSNPQITCTSFDRPNLYLEVGRKTGNILQDLQPFLVKKTSSEWEFEGPTIIYCSSRKMTEKVTAELRKLNLACGTYHAGMSFNTRKDVHHRFMRDEIQCVIATIAFGMGINKSDIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADFNLNRHHFIEIHNEKFRSYKLKMVAKMEKYLHSSRCRRQIILCHFEDRQLQKASLDIMGTENCCDNCRSRLNHCHSINDSSDTSQDFGPQAFLLLSAVDILQGKFGIGVPILFLRGSSSQRLPGPFRRHNLFGAGKDQTESWWKAFSRHLVTEGFLVDVPGGNKFVKICTLTAKGRNWLFKANAESPQRLILQANEELWPKKFLLPSASTVSLGIEHHPSNQIPIELTAEKESNLEKMYSYKALDKISSGINIPKKSIMVSSEPVISAQEQKTQSVLYGKLVEARQRHANKMDVPPAILATNKILLDLAKMRPTTVENVKQIDGVSEGKATMLAPLLEVIKHFCQANSVQADLFSNTKSQEQKKSLEMKNENCSLPKCVAVTYSLFQEKKMSLDSIAKDRILPLTAVGMHLAQAVKAGYSLDMERAGLTPEIQKIIADVIRNPPINSDMSKIKLIRMLVPENIDTYLIHMVIEILETDHDNRLLCQPSSDSSIKRCFPNSEESCSSSKRSKEDVGTNTKMSPATSKRNLPEWFTKGNETIVDTSKKPVAKTKKTGLFS

Target ORF information:

RefSeq Version	XM_013076106.2
Organism	Heterocephalus glaber(naked mole-rat)
Definition	Heterocephalus glaber Werner syndrome RecQ like helicase (Wrn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013076106.2

ORF Insert Sequence:

ATGAATGAGA AAAAATTGGA AACAATATCA CAACAGCGGA AATTTCCTGA ATGGATGTCT 
ATGCAGAATA AAGGATGTGC TATAGAAGGA AAAAAGGCAC ATATTCAGAA GAGTGTTTTT 
GAAGATGATC TCCCCTTCTT AGAATTCACT GGCTCTATTG TGTATAGTTA TGAAGCTAGT 
GATTGCTCTT TCCTATCAGA AGATATTAGC ATGAGTCTGT CTGCTGGAGA TGTGATAGGA 
TTTGACATGG AATGGCCACC AATATACAAT AAAGGGAAAC TAAGCAGAGT TGCACTAGTT 
CAATTGTGTG TGTCTGAGAG CAAATGTTAC TTGTTTCACA TTTCTTCTAT GTCAGTTTTT 
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT GAAACAATTA AAAAGGCAGG TGTAGGAATC 
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG CTTTGTGGAA 
TTGACTGATG TTGCCAATCA AAAGTTGAAA TGTATGGAGA TCTGGAGCCT CAATGGACTG 
GTTAAACACC TCTTTGGTAA ACAACTTCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG 
AGTATTTTCC CTCTCAGTGA GGATCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT 
CTCATCATTT ATCAAAAATT AGAAAGTTTG GGTGATGCCA TACAGATGTT TGATACAAAT 
AAAGTGGAGG AAATCCTACC TAGTGAAATG AAGAAACAGT TGACTGCAAT CTCTGAGGAG 
GTGATGGATC TGGCTAATCA TTTTCCTGAC ACATGCAGAA AATTGGAAAA TCCACAGAGG 
GTTTCTATAA TATTGAAGAA TATTTCAGAA AATCTATATT CACTGAGGAA GATGATGTTT 
GCCTCTACTA ACACTGAGCC TGAACTGAGG CCCAGAAGTA ATTTTAAGTT ATCATCTTTG 
GAGGATTCAA CTGCCGGTGG ACAACAACAG AAACAAACTA GAGAACATAA AATTTTTACC 
AGAGTTAAAG ATGAGACATG GGAGACAACA CTTGATAAGT TCATTAAACA TGATGGAGAA 
GGTGTACTTG GAAATAAAAT GAAACAAGAA GGTGATGGTT CTGAAGATGG AATAAAAGAC 
AATAAATTGA AAGAAAATAT GGAAAGAACT TGTTTGATGT CATTAGATAA TACAGAATAT 
GAACTCCAAA TTTTGGAACA GCAGGCTCAA GAAAAAAATC TTAGTGATGT TTCTTTTCAA 
ACTGCTGAGC GTTTATCTCC CAGTGATAAC GAAAAAGATT CATCTTATAT AATTGAAAGT 
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTAAATAG TGATGCTATA 
GAAACAATTC ATTCTGAGTG CTCAGAAATA AGAGGAAATT TGAGTCTTCC TACTGAAGAA 
GATGAAGATA AAAATGAAGC CTTTGAAGAG GAAGAAGACC ATTTTTTGCC AGAACCCAAT 
GCAAAGCAAG TTATTTGCCT CAAGACCTAT TTTGGCCATC ATAGTTTTAA ACCGGTTCAG 
TGGAAAGTTA TTCATTCTGT TTTGGAAGAA AGAAGAGATA ATGTTGTTGT CATGGCAACT 
GGATATGGAA AGAGTTTATG CTTCCAGTAC CCACCTGTGT ATGTAGGCAA GATTGGCCTT 
GTCATCTCAC CTCTTATTTC CTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC 
ATTCCAGCTT GTTTCCTTGG ATCAGCACAA TCAAAAAATG TTCTAGAAGA TGTTAAATTA 
GGCAAATATC GAATTATTTA CATAACTCCA GAATTCTGTT CAGGTAACTT GGACCTACTC 
CAGCACCTTG AAGCTAATAT TGGTATCACA CTGATTGCTG TGGATGAGGC TCACTGTATT 
TCTGAATGGG GGCATGATTT TAGAAGTTCA TTCAGGACAC TGGGCTCCCT GAAGACAGCA 
CTCCCACTGG TTCCAATAGT TGCACTCACT GCTACTGCAA GTTCTTCGAT CCAGGAAGAT 
ATTATACATT GCTTAAAACT GAGCAATCCT CAGATTACCT GTACTAGTTT TGATCGACCA 
AATCTGTATT TAGAAGTTGG ACGAAAAACA GGGAATATTC TTCAAGATCT ACAGCCATTT 
CTTGTCAAAA AGACAAGTTC TGAATGGGAA TTTGAAGGTC CAACTATCAT CTATTGTTCT 
TCCAGAAAAA TGACAGAAAA AGTTACTGCT GAACTTAGGA AACTAAATTT AGCCTGTGGA 
ACCTACCATG CAGGCATGAG TTTTAACACA AGGAAAGATG TTCATCACAG GTTCATGAGG 
GATGAAATTC AGTGTGTCAT AGCTACTATA GCTTTTGGAA TGGGCATTAA TAAATCTGAC 
ATTCGCAAAG TCATTCATTA TGGTGCACCT AAGGAAATGG AATCATATTA CCAGGAAATT 
GGTAGAGCTG GCCGTGATGG ACTTCAAAGT TCTTGTCATG TACTGTGGGC TCCAGCAGAC 
TTTAACTTAA ATAGGCACCA CTTTATTGAG ATACATAATG AGAAGTTTCG ATCATACAAA 
TTAAAGATGG TGGCAAAAAT GGAAAAATAT CTTCATTCCA GCAGATGTAG GCGGCAGATC 
ATCTTGTGTC ATTTTGAGGA CAGACAACTA CAAAAGGCCT CCTTGGATAT CATGGGAACT 
GAAAACTGCT GTGATAATTG CAGGTCCAGA TTGAATCATT GCCATTCCAT TAATGATTCA 
AGTGATACAT CACAGGACTT TGGTCCACAA GCATTCCTTC TGTTATCTGC TGTGGACATC 
TTACAGGGAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCGAGGATC TAGTTCTCAG 
CGTCTCCCAG GTCCGTTTCG TAGACACAAC CTTTTTGGTG CTGGCAAGGA TCAAACAGAG 
AGTTGGTGGA AGGCCTTTTC CCGTCACCTT GTAACGGAAG GATTTTTGGT AGACGTTCCT 
GGTGGTAACA AATTTGTAAA GATTTGCACC CTTACGGCAA AGGGTAGGAA TTGGCTTTTT 
AAAGCCAATG CAGAATCTCC TCAGAGACTT ATACTTCAAG CTAATGAAGA GTTGTGGCCG 
AAGAAGTTTC TTCTGCCAAG TGCTAGTACT GTATCTTTGG GCATTGAACA TCATCCCTCT 
AATCAAATAC CAATTGAATT AACTGCAGAA AAGGAGTCTA ACTTGGAGAA GATGTATTCT 
TACAAAGCAC TTGATAAAAT TTCTTCTGGG ATTAACATTC CTAAAAAAAG TATCATGGTA 
TCCTCAGAAC CTGTTATTTC AGCCCAGGAG CAAAAGACCC AGAGTGTGTT GTATGGCAAA 
TTAGTAGAAG CTAGACAGAG ACATGCCAAT AAAATGGATG TTCCTCCAGC TATTCTGGCC 
ACAAACAAGA TACTATTGGA TTTGGCCAAA ATGAGACCAA CTACAGTTGA AAATGTAAAA 
CAAATTGATG GTGTATCTGA AGGCAAAGCT ACTATGTTGG CCCCTCTGTT GGAAGTCATC 
AAGCATTTCT GCCAAGCAAA TAGTGTTCAG GCAGACCTCT TTTCAAATAC AAAGTCTCAA 
GAACAGAAGA AAAGTTTGGA AATGAAGAAT GAAAACTGCT CCCTCCCGAA GTGTGTGGCT 
GTCACATATT CTTTATTCCA AGAAAAGAAG ATGTCTTTGG ACAGCATAGC CAAGGACAGG 
ATTCTGCCGC TCACAGCAGT TGGCATGCAC TTAGCCCAAG CAGTGAAAGC TGGCTACTCC 
CTGGATATGG AGCGGGCAGG GCTGACTCCA GAGATTCAGA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATATGAGT AAAATTAAAC TAATTAGAAT GTTAGTTCCT 
GAAAACATCG ACACATACCT TATCCACATG GTAATTGAGA TTCTTGAAAC TGATCATGAC 
AACAGACTGT TATGCCAGCC TTCAAGTGAT TCCAGCATAA AGAGATGTTT CCCCAACTCT 
GAAGAGAGCT GTTCAAGTTC TAAAAGAAGC AAGGAAGATG TAGGCACTAA TACCAAGATG 
TCACCTGCAA CCTCAAAGAG AAACTTACCT GAGTGGTTTA CCAAAGGAAA TGAGACCATT 
GTCGATACAA GCAAGAAACC AGTGGCCAAA ACAAAAAAGA CAGGTCTTTT TAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

Wrn cDNA ORF clone, Heterocephalus glaber(naked mole-rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム