Nelfe cDNA ORF clone, Heterocephalus glaber(naked mole-rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nelfe
Entrez Gene ID	101713224
Full Name	negative elongation factor complex member E
Gene Type	protein-coding
Organism	Heterocephalus glaber(naked mole-rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004847162.2	XP_004847219.1	negative elongation factor E
XM_021259444.1	XP_021115103.1	negative elongation factor E

Pathways from BioSystems
Gene Ontology

The following Nelfe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nelfe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHe231665	XM_021259444.1 Latest version!	Heterocephalus glaber negative elongation factor complex member E (Nelfe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OHe41155	XM_004847162.2 Latest version!	Heterocephalus glaber negative elongation factor complex member E (Nelfe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nelfe ( XM_021259444.1 ) cDNA ORF clone, Heterocephalus glaber(naked mole-rat) -> XP_021115103.1 Heterocephalus glaber negative elongation factor complex member E (Nelfe), mRNA.

Price & Availability↑

CloneID	OHe231665
Clone ID Related Accession (Same CDS sequence)	XM_021259444.1
Accession Version	XM_021259444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-22
Organism	Heterocephalus glaber(naked mole-rat)
Product	negative elongation factor E
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004624754.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Heterocephalus glaber Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases ##RefSeq-Attributes-END##

ATGTTGGTGA TCCCCCCCGG ACTGAGCGAG GAGGAGGAGG CTCTGCAGAA GAAATTCAGC 
AAGCTCAAGA AGAAGGTGAG GGAGTGTGTG GGGCCCTGGC CCAACCTTTC ACAGATTCTG 
CACTCTGAGC ACACCTGGGC CTCCTGCTGC GACACTGACC TGAGCTGCTT CTGCCTCCAG 
GACCCCGAGA AGGGCCCGGC GCCCACCTTC CAGCCATTCC AGAGGAGCAT GTCCGCGGAC 
GACAACCTGC AGGAGGCGTC CAGGCGTCCC CAGAGGAAGC CGCTCTATGA GAGCTTTGTG 
TCTTCCAGTG ATCGGCTCCG AGAACTGGGG CCAGATGGAG AAGAAGCCGA GGGCCCAGGG 
GCCGGCGATG GCCCTCCGCG GAGCTTTGAC TGGGGCTACG AAGAGCATGG TGGTGCCCGC 
TCCTCGGCCT CCCCTCCCCG CAGCCGGGAC CGCAGCCACG AGAGGAGCCG GGACAGAGAC 
CGAGAGCGGG AGCGGGAGCG GGAGCGGGAG CGGGAGCGGG AGCGGGAGCA GCGGGACAGA 
GACCGGGAAC AGCGGGACAG AGATAAAGAC CGGGAGCGGG AGCGGGAGCG GGACCGAGAG 
GGCCCTTTCC GCAGGTCGGA CTCCTTCCCG GAGCGCCGGG CCCCTCGGAA GGGGAACACT 
CTCTACGTCT ATGGAGAAGA CATGACCCCC ACGCTGCTCC GCGGAGCTTT CGCTCCCTTC 
GGGAACATCA TTGACCTCTC CATGGACCCG CCTAGAAACT GTGCCTTCGT CACCTACGAA 
AAGATGGAGT CCGCAGACCA GGCCGTGGCC GAGCTCAACG GGACCCAGGT GGAGTCCGTG 
CAGCTCAAGG TCAACATAGC GCGCAAACAG CCCATGCTGG ACGCCGCCAC CGGCAAGTCT 
GTGTGGGGCT CCCTCGCTGT CCAGAACAGC CCTAAGGGTT GCCACCGGGA CAAGAGGACC 
CAGATTGTCT ACAGTGATGA TGTCTACAAG GAAAACCTTG TGGATGGCTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021115103.1
CDS	158..1171
Translation	MLVIPPGLSEEEEALQKKFSKLKKKVRECVGPWPNLSQILHSEHTWASCCDTDLSCFCLQDPEKGPAPTFQPFQRSMSADDNLQEASRRPQRKPLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEEHGGARSSASPPRSRDRSHERSRDRDREREREREREREREREQRDRDREQRDRDKDRERERERDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFAPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF

Target ORF information:

RefSeq Version	XM_021259444.1
Organism	Heterocephalus glaber(naked mole-rat)
Definition	Heterocephalus glaber negative elongation factor complex member E (Nelfe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021259444.1

ORF Insert Sequence:

ATGTTGGTGA TCCCCCCCGG ACTGAGCGAG GAGGAGGAGG CTCTGCAGAA GAAATTCAGC 
AAGCTCAAGA AGAAGGTGAG GGAGTGTGTG GGGCCCTGGC CCAACCTTTC ACAGATTCTG 
CACTCTGAGC ACACCTGGGC CTCCTGCTGC GACACTGACC TGAGCTGCTT CTGCCTCCAG 
GACCCCGAGA AGGGCCCGGC GCCCACCTTC CAGCCATTCC AGAGGAGCAT GTCCGCGGAC 
GACAACCTGC AGGAGGCGTC CAGGCGTCCC CAGAGGAAGC CGCTCTATGA GAGCTTTGTG 
TCTTCCAGTG ATCGGCTCCG AGAACTGGGG CCAGATGGAG AAGAAGCCGA GGGCCCAGGG 
GCCGGCGATG GCCCTCCGCG GAGCTTTGAC TGGGGCTACG AAGAGCATGG TGGTGCCCGC 
TCCTCGGCCT CCCCTCCCCG CAGCCGGGAC CGCAGCCACG AGAGGAGCCG GGACAGAGAC 
CGAGAGCGGG AGCGGGAGCG GGAGCGGGAG CGGGAGCGGG AGCGGGAGCA GCGGGACAGA 
GACCGGGAAC AGCGGGACAG AGATAAAGAC CGGGAGCGGG AGCGGGAGCG GGACCGAGAG 
GGCCCTTTCC GCAGGTCGGA CTCCTTCCCG GAGCGCCGGG CCCCTCGGAA GGGGAACACT 
CTCTACGTCT ATGGAGAAGA CATGACCCCC ACGCTGCTCC GCGGAGCTTT CGCTCCCTTC 
GGGAACATCA TTGACCTCTC CATGGACCCG CCTAGAAACT GTGCCTTCGT CACCTACGAA 
AAGATGGAGT CCGCAGACCA GGCCGTGGCC GAGCTCAACG GGACCCAGGT GGAGTCCGTG 
CAGCTCAAGG TCAACATAGC GCGCAAACAG CCCATGCTGG ACGCCGCCAC CGGCAAGTCT 
GTGTGGGGCT CCCTCGCTGT CCAGAACAGC CCTAAGGGTT GCCACCGGGA CAAGAGGACC 
CAGATTGTCT ACAGTGATGA TGTCTACAAG GAAAACCTTG TGGATGGCTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nelfe ( XM_004847162.2 ) cDNA ORF clone, Heterocephalus glaber(naked mole-rat) -> XP_004847219.1 Heterocephalus glaber negative elongation factor complex member E (Nelfe), mRNA.

Price & Availability↑

CloneID	OHe41155
Clone ID Related Accession (Same CDS sequence)	XM_004847162.2
Accession Version	XM_004847162.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-01
Organism	Heterocephalus glaber(Naked mole-rat)
Product	negative elongation factor E
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004624754.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 2, 2015 this sequence version replaced XM_004847162.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Heterocephalus glaber Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGA TCCCCCCCGG ACTGAGCGAG GAGGAGGAGG CTCTGCAGAA GAAATTCAGC 
AAGCTCAAGA AGAAGGACCC CGAGAAGGGC CCGGCGCCCA CCTTCCAGCC ATTCCAGAGG 
AGCATGTCCG CGGACGACAA CCTGCAGGAG GCGTCCAGGC GTCCCCAGAG GAAGCCGCTC 
TATGAGAGCT TTGTGTCTTC CAGTGATCGG CTCCGAGAAC TGGGGCCAGA TGGAGAAGAA 
GCCGAGGGCC CAGGGGCCGG CGATGGCCCT CCGCGGAGCT TTGACTGGGG CTACGAAGAG 
CATGGTGGTG CCCGCTCCTC GGCCTCCCCT CCCCGCAGCC GGGACCGCAG CCACGAGAGG 
AGCCGGGACA GAGACCGAGA GCGGGAGCGG GAGCGGGAGC GGGAGCGGGA GCGGGAGCGG 
GAGCAGCGGG ACAGAGACCG GGAACAGCGG GACAGAGATA AAGACCGGGA GCGGGAGCGG 
GAGCGGGACC GAGAGGGCCC TTTCCGCAGG TCGGACTCCT TCCCGGAGCG CCGGGCCCCT 
CGGAAGGGGA ACACTCTCTA CGTCTATGGA GAAGACATGA CCCCCACGCT GCTCCGCGGA 
GCTTTCGCTC CCTTCGGGAA CATCATTGAC CTCTCCATGG ACCCGCCTAG AAACTGTGCC 
TTCGTCACCT ACGAAAAGAT GGAGTCCGCA GACCAGGCCG TGGCCGAGCT CAACGGGACC 
CAGGTGGAGT CCGTGCAGCT CAAGGTCAAC ATAGCGCGCA AACAGCCCAT GCTGGACGCC 
GCCACCGGCA AGTCTGTGTG GGGCTCCCTC GCTGTCCAGA ACAGCCCTAA GGGTTGCCAC 
CGGGACAAGA GGACCCAGAT TGTCTACAGT GATGATGTCT ACAAGGAAAA CCTTGTGGAT 
GGCTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004847219.1
CDS	81..989
Translation	MLVIPPGLSEEEEALQKKFSKLKKKDPEKGPAPTFQPFQRSMSADDNLQEASRRPQRKPLYESFVSSSDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEEHGGARSSASPPRSRDRSHERSRDRDREREREREREREREREQRDRDREQRDRDKDRERERERDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFAPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF

Target ORF information:

RefSeq Version	XM_004847162.2
Organism	Heterocephalus glaber(Naked mole-rat)
Definition	Heterocephalus glaber negative elongation factor complex member E (Nelfe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004847162.2

ORF Insert Sequence:

ATGTTGGTGA TCCCCCCCGG ACTGAGCGAG GAGGAGGAGG CTCTGCAGAA GAAATTCAGC 
AAGCTCAAGA AGAAGGACCC CGAGAAGGGC CCGGCGCCCA CCTTCCAGCC ATTCCAGAGG 
AGCATGTCCG CGGACGACAA CCTGCAGGAG GCGTCCAGGC GTCCCCAGAG GAAGCCGCTC 
TATGAGAGCT TTGTGTCTTC CAGTGATCGG CTCCGAGAAC TGGGGCCAGA TGGAGAAGAA 
GCCGAGGGCC CAGGGGCCGG CGATGGCCCT CCGCGGAGCT TTGACTGGGG CTACGAAGAG 
CATGGTGGTG CCCGCTCCTC GGCCTCCCCT CCCCGCAGCC GGGACCGCAG CCACGAGAGG 
AGCCGGGACA GAGACCGAGA GCGGGAGCGG GAGCGGGAGC GGGAGCGGGA GCGGGAGCGG 
GAGCAGCGGG ACAGAGACCG GGAACAGCGG GACAGAGATA AAGACCGGGA GCGGGAGCGG 
GAGCGGGACC GAGAGGGCCC TTTCCGCAGG TCGGACTCCT TCCCGGAGCG CCGGGCCCCT 
CGGAAGGGGA ACACTCTCTA CGTCTATGGA GAAGACATGA CCCCCACGCT GCTCCGCGGA 
GCTTTCGCTC CCTTCGGGAA CATCATTGAC CTCTCCATGG ACCCGCCTAG AAACTGTGCC 
TTCGTCACCT ACGAAAAGAT GGAGTCCGCA GACCAGGCCG TGGCCGAGCT CAACGGGACC 
CAGGTGGAGT CCGTGCAGCT CAAGGTCAAC ATAGCGCGCA AACAGCCCAT GCTGGACGCC 
GCCACCGGCA AGTCTGTGTG GGGCTCCCTC GCTGTCCAGA ACAGCCCTAA GGGTTGCCAC 
CGGGACAAGA GGACCCAGAT TGTCTACAGT GATGATGTCT ACAAGGAAAA CCTTGTGGAT 
GGCTTTTAG