LOC102312366 cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC102312366
Entrez Gene ID	102312366
Full Name	probable E3 ubiquitin-protein ligase HERC6
Gene Type	protein-coding
Organism	Haplochromis burtoni(Burton's mouthbrooder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005915949.1	XP_005916011.1	probable E3 ubiquitin-protein ligase HERC6 isoform X1
XM_005915950.1	XP_005916012.1	probable E3 ubiquitin-protein ligase HERC6 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC102312366 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC102312366 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHa03582	XM_005915949.1 Latest version!	Haplochromis burtoni probable E3 ubiquitin-protein ligase HERC6 (LOC102312366), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OHa03583	XM_005915950.1 Latest version!	Haplochromis burtoni probable E3 ubiquitin-protein ligase HERC6 (LOC102312366), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC102312366 ( XM_005915949.1 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_005916011.1 Haplochromis burtoni probable E3 ubiquitin-protein ligase HERC6 (LOC102312366), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHa03582
Clone ID Related Accession (Same CDS sequence)	XM_005915949.1
Accession Version	XM_005915949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005179412.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCTGT TCTGTTGGGG AGACAGCTCC AGCGGACAGT TCGGGTCACA GGCGGCCCCC 
AGCCCCGTGT CCTGGACCGT CCCCGGGGTT GTAACTAAGA TCTGCTGTGG GGATCGGCAC 
ACTTTATTTC TTAATGCAGA CGGGGACGTG CTGTCATGTG GGCACAACTC TCAGGGACAA 
CTTGGACGAC AGAAGAAAAA AAATGGAAAG ATCATAGCTC AAGTGGAGGG GCTGGGCCGT 
GTGGTGGCGA TGGCTTGCGG TCAGGACCAC TCTCTGGCTG TGAGTGCATC TGGACACGTT 
TTCTCCTGGG GGGCAGGAGA GGATGGACAG CTAGGGTTAC CAACACCTTC CAACAGTCAC 
AGACCAAGCA AGGTGCCTAT CCCACTGCCC ATACCGATAA TTCAAGTTGC TTGTGGAAAC 
TCCCATTCCC TTGCCCTGAC TAAAGGAGGC AATGTCTTCT CGTGGGGTTT AAACAGTCAT 
GGTCAGCTGG GCCTGGGGAA GGAGGTGCCG GTGCAGGATA CACCCGTCAT AATTTGTGCT 
TTGGCTGGAG TAGCGGTAAC CCAGATTGCA GCAGGAGGGA CCCACAGTCT GTTCCTTACC 
CTCGCAGACC TGGTGTACTG CTGTGGGGCC AATAAATGTG GTCAGCTCGG GGTTAACAGG 
GTGGATGAGA AAGGCAGGTT CAACATCTGT ATGGTGCCTG CGCTCAGACC TCTAAGTGTC 
TCCTTCATAA GCTGTGGAGA GTCGCACTCT GCTGTGCTGA CAAAGGACGG TAAAGTTTAC 
ACTTTTGGAG ACGGAAGCCA AGGTCAGCTC GGTCACAGCA CCTCTGCTAA TGAAGTGAAG 
CCCAAACTGG TTGCAGGTGT GGATGGTCCT GCTTCCCAGA TTGCATGTGG CAGGCATCAC 
ACTCTGGTTT TGGGCTCTTC TGGTCAGCTC TGGGCTTTTG GCAGTGGAGC TAAAGGTCAA 
ACTGGAAATG GGCACAATGG TGGCAGTCTG ACTCCCACTC TGGTTCAGTT TCCATGGACC 
ACTGACGATG CAGCAGCCAT GCCCAGTGCA GACTTGAAAA TAGCTGCTGG GTGGAACACA 
AACTTTGCTT ACTCTTCACC TGCAGAGAAC TCTGTTCACA AACAGATAAC TGGACGTCTT 
GATGAGACAA AACTCCAAGA ATGGCTGATG ATGGAGCATA GTAATGTGGA GGCAAAAAGA 
GAAATCGGAT CGATGTTTCT AACAAGTTCA AGTCTTGTTG CAAGTTTCAC AAAAGCCAAT 
GGGTTTCCAC TGGAAGCAGG CGACTTAACC GTGGACCTGG AGGCCGCTAG CCGAGCTTTC 
GACCAGCTGT TCACAAGACA GTGGATCAAA CAATCAGTAA ACTTGGTCCC TCTGTGCGAC 
TTGCTTTACC TGTCAAGAAC TGCATTAAAA TCTCCAGAGA TCATCCTGAT TCTTCTGACC 
TGCCCGCTTC TTCAAGAGGA CTCTAATGTC ATGAATACAG TTCTATCTGT GGCCATCACT 
ATAGCAGAGA TGTCGGACAA GACTCTGGAA ACATTAAAAA AGTGGTGGAG TTCACTGTCC 
CCCTCGATAC TGATGAAGCA CATCATGGTC TTCAAAAAGG CTCTCGCCTT CATGCTAAAG 
AATGGCCTCT TACAAACACA CAACCCAGGA GTCAAATTTA TCCTGGAAGC CCTCAAGCTG 
CTATACAAAG CAAACAAAGC TGGAAAGTCC TACAAAGTCC CACTGAGCAC CTTTTATGTA 
GAAGAAATCA CAAATGCTGT GCAGCTTGAC ACTGACATCA TGCTTTGGTG GCAATTCTCC 
AAAGAGGAGG ATGACGTGAA CACACCTGCC ATCTTCTGTC GCTACCCATT CCTTTTGACC 
CTGCTTCACA AAGTGGCCGT ATTTAACTTC TGTGCAGCCA CAGTGAAGAA TGTGCATCAA 
CTCGCTCATC AAGCAGCTTT GGTGCCCCAT CAACCGCACG CCCCAGACTC TCCACCTGCC 
CCAGTCTTCC AGCTAACCCT GAGGCGATCT CACCTGGTCG AGGACACCTT CAGACAGCTC 
AGTGCAGCCG ACCACTGTGC ATTCCAAAGG GAGCTTTTAG TGCTGTTTGT GGAGGACAGG 
AAGACAACAA ATGTCAACAA AAGGGACTTT TTCCTGCACG TGTTTGATGA GCTGATGGCT 
CCAGAATCTA AAATGTTCAT ATATAATGAG AGCCAGACTG TGGCCTGGTT CCCTCCCAAG 
CCAAAAGTAG AGGAGAAGAC TTACTTCCTG TTTGGCGTTC TGTGTGGCCT GGCTCTCTAC 
AACCACAACA TTATCCACCT GCGCTTCCCA CAAGCTCTGT TCAAGAAGCT GCTCAGGGTC 
AAACCTACGC TGGACGACAT GAAGGAGTTT GACCCTGTGC TGGCAGAGTC TTGGAGGATC 
ATCTTAGAGG ACTACACCCC TGATGTTGTT CAAACGCTGG ACACTACTTT CACTGTGTCC 
TGGGCTGGTG AGACAGTTGA GCTTGATCCA AATGAACCAG GAAAACTTGT CACCGGGTCA 
AACAGGAAAG AATACGTAGC TGCCTTTGTG AACTACGTCT TCAACAAATC AGTGGAGAGA 
GTGTTTGACG AGTTCAAGAG AGGGTTTTTC AAAGTGTGCA ACATGGACGT GGTGGACTTC 
TTCCAGCCAG AGGAGCTGCA AGCAGTGATG GTGGGCCAAG AAAACTACGA CTGGGAGGTG 
TTCAAGAAGA ACACATTTTA TGAAGGCGAA TACCATGATG AGCATCCAAA CATACTGGTG 
TTCTGGGAGG TGTTTGAGAG CCTGACAGCA GAGGAAAAGA AAAAGTTCCT TTTGTTTCTC 
ACAGGCAGTG ATCGCGTACC CTTCCTGGGA ATGGAGAGCA TCAAGATGAG AGTGGCTGTG 
CTGCCCGATG CTTCTGAACG CCATTTGCCT GAATCGCTTA CCTGCCACTT CCTGTTGTTA 
CTGCCCATCT ACAGGAGATA CCCAGTCGAG AGGACGATGC GCACAAGGCT TCTCCAGGCT 
ATCAATCACA ACAGAGGCTT CCGGAAGAAG CGACACACCA CAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005916011.1
CDS	59..3106
Translation	MQLFCWGDSSSGQFGSQAAPSPVSWTVPGVVTKICCGDRHTLFLNADGDVLSCGHNSQGQLGRQKKKNGKIIAQVEGLGRVVAMACGQDHSLAVSASGHVFSWGAGEDGQLGLPTPSNSHRPSKVPIPLPIPIIQVACGNSHSLALTKGGNVFSWGLNSHGQLGLGKEVPVQDTPVIICALAGVAVTQIAAGGTHSLFLTLADLVYCCGANKCGQLGVNRVDEKGRFNICMVPALRPLSVSFISCGESHSAVLTKDGKVYTFGDGSQGQLGHSTSANEVKPKLVAGVDGPASQIACGRHHTLVLGSSGQLWAFGSGAKGQTGNGHNGGSLTPTLVQFPWTTDDAAAMPSADLKIAAGWNTNFAYSSPAENSVHKQITGRLDETKLQEWLMMEHSNVEAKREIGSMFLTSSSLVASFTKANGFPLEAGDLTVDLEAASRAFDQLFTRQWIKQSVNLVPLCDLLYLSRTALKSPEIILILLTCPLLQEDSNVMNTVLSVAITIAEMSDKTLETLKKWWSSLSPSILMKHIMVFKKALAFMLKNGLLQTHNPGVKFILEALKLLYKANKAGKSYKVPLSTFYVEEITNAVQLDTDIMLWWQFSKEEDDVNTPAIFCRYPFLLTLLHKVAVFNFCAATVKNVHQLAHQAALVPHQPHAPDSPPAPVFQLTLRRSHLVEDTFRQLSAADHCAFQRELLVLFVEDRKTTNVNKRDFFLHVFDELMAPESKMFIYNESQTVAWFPPKPKVEEKTYFLFGVLCGLALYNHNIIHLRFPQALFKKLLRVKPTLDDMKEFDPVLAESWRIILEDYTPDVVQTLDTTFTVSWAGETVELDPNEPGKLVTGSNRKEYVAAFVNYVFNKSVERVFDEFKRGFFKVCNMDVVDFFQPEELQAVMVGQENYDWEVFKKNTFYEGEYHDEHPNILVFWEVFESLTAEEKKKFLLFLTGSDRVPFLGMESIKMRVAVLPDASERHLPESLTCHFLLLLPIYRRYPVERTMRTRLLQAINHNRGFRKKRHTTE

Target ORF information:

RefSeq Version	XM_005915949.1
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni probable E3 ubiquitin-protein ligase HERC6 (LOC102312366), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005915949.1

ORF Insert Sequence:

ATGCAGCTGT TCTGTTGGGG AGACAGCTCC AGCGGACAGT TCGGGTCACA GGCGGCCCCC 
AGCCCCGTGT CCTGGACCGT CCCCGGGGTT GTAACTAAGA TCTGCTGTGG GGATCGGCAC 
ACTTTATTTC TTAATGCAGA CGGGGACGTG CTGTCATGTG GGCACAACTC TCAGGGACAA 
CTTGGACGAC AGAAGAAAAA AAATGGAAAG ATCATAGCTC AAGTGGAGGG GCTGGGCCGT 
GTGGTGGCGA TGGCTTGCGG TCAGGACCAC TCTCTGGCTG TGAGTGCATC TGGACACGTT 
TTCTCCTGGG GGGCAGGAGA GGATGGACAG CTAGGGTTAC CAACACCTTC CAACAGTCAC 
AGACCAAGCA AGGTGCCTAT CCCACTGCCC ATACCGATAA TTCAAGTTGC TTGTGGAAAC 
TCCCATTCCC TTGCCCTGAC TAAAGGAGGC AATGTCTTCT CGTGGGGTTT AAACAGTCAT 
GGTCAGCTGG GCCTGGGGAA GGAGGTGCCG GTGCAGGATA CACCCGTCAT AATTTGTGCT 
TTGGCTGGAG TAGCGGTAAC CCAGATTGCA GCAGGAGGGA CCCACAGTCT GTTCCTTACC 
CTCGCAGACC TGGTGTACTG CTGTGGGGCC AATAAATGTG GTCAGCTCGG GGTTAACAGG 
GTGGATGAGA AAGGCAGGTT CAACATCTGT ATGGTGCCTG CGCTCAGACC TCTAAGTGTC 
TCCTTCATAA GCTGTGGAGA GTCGCACTCT GCTGTGCTGA CAAAGGACGG TAAAGTTTAC 
ACTTTTGGAG ACGGAAGCCA AGGTCAGCTC GGTCACAGCA CCTCTGCTAA TGAAGTGAAG 
CCCAAACTGG TTGCAGGTGT GGATGGTCCT GCTTCCCAGA TTGCATGTGG CAGGCATCAC 
ACTCTGGTTT TGGGCTCTTC TGGTCAGCTC TGGGCTTTTG GCAGTGGAGC TAAAGGTCAA 
ACTGGAAATG GGCACAATGG TGGCAGTCTG ACTCCCACTC TGGTTCAGTT TCCATGGACC 
ACTGACGATG CAGCAGCCAT GCCCAGTGCA GACTTGAAAA TAGCTGCTGG GTGGAACACA 
AACTTTGCTT ACTCTTCACC TGCAGAGAAC TCTGTTCACA AACAGATAAC TGGACGTCTT 
GATGAGACAA AACTCCAAGA ATGGCTGATG ATGGAGCATA GTAATGTGGA GGCAAAAAGA 
GAAATCGGAT CGATGTTTCT AACAAGTTCA AGTCTTGTTG CAAGTTTCAC AAAAGCCAAT 
GGGTTTCCAC TGGAAGCAGG CGACTTAACC GTGGACCTGG AGGCCGCTAG CCGAGCTTTC 
GACCAGCTGT TCACAAGACA GTGGATCAAA CAATCAGTAA ACTTGGTCCC TCTGTGCGAC 
TTGCTTTACC TGTCAAGAAC TGCATTAAAA TCTCCAGAGA TCATCCTGAT TCTTCTGACC 
TGCCCGCTTC TTCAAGAGGA CTCTAATGTC ATGAATACAG TTCTATCTGT GGCCATCACT 
ATAGCAGAGA TGTCGGACAA GACTCTGGAA ACATTAAAAA AGTGGTGGAG TTCACTGTCC 
CCCTCGATAC TGATGAAGCA CATCATGGTC TTCAAAAAGG CTCTCGCCTT CATGCTAAAG 
AATGGCCTCT TACAAACACA CAACCCAGGA GTCAAATTTA TCCTGGAAGC CCTCAAGCTG 
CTATACAAAG CAAACAAAGC TGGAAAGTCC TACAAAGTCC CACTGAGCAC CTTTTATGTA 
GAAGAAATCA CAAATGCTGT GCAGCTTGAC ACTGACATCA TGCTTTGGTG GCAATTCTCC 
AAAGAGGAGG ATGACGTGAA CACACCTGCC ATCTTCTGTC GCTACCCATT CCTTTTGACC 
CTGCTTCACA AAGTGGCCGT ATTTAACTTC TGTGCAGCCA CAGTGAAGAA TGTGCATCAA 
CTCGCTCATC AAGCAGCTTT GGTGCCCCAT CAACCGCACG CCCCAGACTC TCCACCTGCC 
CCAGTCTTCC AGCTAACCCT GAGGCGATCT CACCTGGTCG AGGACACCTT CAGACAGCTC 
AGTGCAGCCG ACCACTGTGC ATTCCAAAGG GAGCTTTTAG TGCTGTTTGT GGAGGACAGG 
AAGACAACAA ATGTCAACAA AAGGGACTTT TTCCTGCACG TGTTTGATGA GCTGATGGCT 
CCAGAATCTA AAATGTTCAT ATATAATGAG AGCCAGACTG TGGCCTGGTT CCCTCCCAAG 
CCAAAAGTAG AGGAGAAGAC TTACTTCCTG TTTGGCGTTC TGTGTGGCCT GGCTCTCTAC 
AACCACAACA TTATCCACCT GCGCTTCCCA CAAGCTCTGT TCAAGAAGCT GCTCAGGGTC 
AAACCTACGC TGGACGACAT GAAGGAGTTT GACCCTGTGC TGGCAGAGTC TTGGAGGATC 
ATCTTAGAGG ACTACACCCC TGATGTTGTT CAAACGCTGG ACACTACTTT CACTGTGTCC 
TGGGCTGGTG AGACAGTTGA GCTTGATCCA AATGAACCAG GAAAACTTGT CACCGGGTCA 
AACAGGAAAG AATACGTAGC TGCCTTTGTG AACTACGTCT TCAACAAATC AGTGGAGAGA 
GTGTTTGACG AGTTCAAGAG AGGGTTTTTC AAAGTGTGCA ACATGGACGT GGTGGACTTC 
TTCCAGCCAG AGGAGCTGCA AGCAGTGATG GTGGGCCAAG AAAACTACGA CTGGGAGGTG 
TTCAAGAAGA ACACATTTTA TGAAGGCGAA TACCATGATG AGCATCCAAA CATACTGGTG 
TTCTGGGAGG TGTTTGAGAG CCTGACAGCA GAGGAAAAGA AAAAGTTCCT TTTGTTTCTC 
ACAGGCAGTG ATCGCGTACC CTTCCTGGGA ATGGAGAGCA TCAAGATGAG AGTGGCTGTG 
CTGCCCGATG CTTCTGAACG CCATTTGCCT GAATCGCTTA CCTGCCACTT CCTGTTGTTA 
CTGCCCATCT ACAGGAGATA CCCAGTCGAG AGGACGATGC GCACAAGGCT TCTCCAGGCT 
ATCAATCACA ACAGAGGCTT CCGGAAGAAG CGACACACCA CAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102312366 ( XM_005915950.1 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_005916012.1 Haplochromis burtoni probable E3 ubiquitin-protein ligase HERC6 (LOC102312366), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHa03583
Clone ID Related Accession (Same CDS sequence)	XM_005915950.1
Accession Version	XM_005915950.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	probable E3 ubiquitin-protein ligase HERC6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005179412.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCTGT TCTGTTGGGG AGACAGCTCC AGCGGACAGT TCGGGTCACA GGCGGCCCCC 
AGCCCCGTGT CCTGGACCGT CCCCGGGGTT GTAACTAAGA TCTGCTGTGG GGATCGGCAC 
ACTTTATTTC TTAATGCAGA CGGGGACGTG CTGTCATGTG GGCACAACTC TCAGGGACAA 
CTTGGACGAC AGAAGAAAAA AAATGGAAAG ATCATAGCTC AAGTGGAGGG GCTGGGCCGT 
GTGGTGGCGA TGGCTTGCGG TCAGGACCAC TCTCTGGCTG TGAGTGCATC TGGACACGTT 
TTCTCCTGGG GGGCAGGAGA GGATGGACAG CTAGGGTTAC CAACACCTTC CAACAGTCAC 
AGACCAAGCA AGGTGCCTAT CCCACTGCCC ATACCGATAA TTCAAGTTGC TTGTGGAAAC 
TCCCATTCCC TTGCCCTGAC TAAAGGAGGC AATGTCTTCT CGTGGGGTTT AAACAGTCAT 
GGTCAGCTGG GCCTGGGGAA GGAGGTGCCG GTGCAGGATA CACCCGTCAT AATTTGTGCT 
TTGGCTGGAG TAGCGGTAAC CCAGATTGCA GCAGGAGGGA CCCACAGTCT GTTCCTTACC 
CTCGCAGACC TGGTGTACTG CTGTGGGGCC AATAAATGTG GTCAGCTCGG GGTTAACAGG 
GTGGATGAGA AAGGCAGGTT CAACATCTGT ATGGTGCCTG CGCTCAGACC TCTAAGTGTC 
TCCTTCATAA GCTGTGGAGA GTCGCACTCT GCTGTGCTGA CAAAGGACGG TAAAGTTTAC 
ACTTTTGGAG ACGGAAGCCA AGGTCAGCTC GGTCACAGCA CCTCTGCTAA TGAAGTGAAG 
CCCAAACTGG TTGCAGGTGT GGATGGTCCT GCTTCCCAGA TTGCATGTGG CAGGCATCAC 
ACTCTGGTTT TGGGCTCTTC TGGTCAGCTC TGGGCTTTTG GCAGTGGAGC TAAAGGTCAA 
ACTGGAAATG GGCACAATGG TGGCAGTCTG ACTCCCACTC TGGTTCAGTT TCCATGGACC 
ACTGACGATG CAGCAGCCAT GCCCAGTGAC TTGAAAATAG CTGCTGGGTG GAACACAAAC 
TTTGCTTACT CTTCACCTGC AGAGAACTCT GTTCACAAAC AGATAACTGG ACGTCTTGAT 
GAGACAAAAC TCCAAGAATG GCTGATGATG GAGCATAGTA ATGTGGAGGC AAAAAGAGAA 
ATCGGATCGA TGTTTCTAAC AAGTTCAAGT CTTGTTGCAA GTTTCACAAA AGCCAATGGG 
TTTCCACTGG AAGCAGGCGA CTTAACCGTG GACCTGGAGG CCGCTAGCCG AGCTTTCGAC 
CAGCTGTTCA CAAGACAGTG GATCAAACAA TCAGTAAACT TGGTCCCTCT GTGCGACTTG 
CTTTACCTGT CAAGAACTGC ATTAAAATCT CCAGAGATCA TCCTGATTCT TCTGACCTGC 
CCGCTTCTTC AAGAGGACTC TAATGTCATG AATACAGTTC TATCTGTGGC CATCACTATA 
GCAGAGATGT CGGACAAGAC TCTGGAAACA TTAAAAAAGT GGTGGAGTTC ACTGTCCCCC 
TCGATACTGA TGAAGCACAT CATGGTCTTC AAAAAGGCTC TCGCCTTCAT GCTAAAGAAT 
GGCCTCTTAC AAACACACAA CCCAGGAGTC AAATTTATCC TGGAAGCCCT CAAGCTGCTA 
TACAAAGCAA ACAAAGCTGG AAAGTCCTAC AAAGTCCCAC TGAGCACCTT TTATGTAGAA 
GAAATCACAA ATGCTGTGCA GCTTGACACT GACATCATGC TTTGGTGGCA ATTCTCCAAA 
GAGGAGGATG ACGTGAACAC ACCTGCCATC TTCTGTCGCT ACCCATTCCT TTTGACCCTG 
CTTCACAAAG TGGCCGTATT TAACTTCTGT GCAGCCACAG TGAAGAATGT GCATCAACTC 
GCTCATCAAG CAGCTTTGGT GCCCCATCAA CCGCACGCCC CAGACTCTCC ACCTGCCCCA 
GTCTTCCAGC TAACCCTGAG GCGATCTCAC CTGGTCGAGG ACACCTTCAG ACAGCTCAGT 
GCAGCCGACC ACTGTGCATT CCAAAGGGAG CTTTTAGTGC TGTTTGTGGA GGACAGGAAG 
ACAACAAATG TCAACAAAAG GGACTTTTTC CTGCACGTGT TTGATGAGCT GATGGCTCCA 
GAATCTAAAA TGTTCATATA TAATGAGAGC CAGACTGTGG CCTGGTTCCC TCCCAAGCCA 
AAAGTAGAGG AGAAGACTTA CTTCCTGTTT GGCGTTCTGT GTGGCCTGGC TCTCTACAAC 
CACAACATTA TCCACCTGCG CTTCCCACAA GCTCTGTTCA AGAAGCTGCT CAGGGTCAAA 
CCTACGCTGG ACGACATGAA GGAGTTTGAC CCTGTGCTGG CAGAGTCTTG GAGGATCATC 
TTAGAGGACT ACACCCCTGA TGTTGTTCAA ACGCTGGACA CTACTTTCAC TGTGTCCTGG 
GCTGGTGAGA CAGTTGAGCT TGATCCAAAT GAACCAGGAA AACTTGTCAC CGGGTCAAAC 
AGGAAAGAAT ACGTAGCTGC CTTTGTGAAC TACGTCTTCA ACAAATCAGT GGAGAGAGTG 
TTTGACGAGT TCAAGAGAGG GTTTTTCAAA GTGTGCAACA TGGACGTGGT GGACTTCTTC 
CAGCCAGAGG AGCTGCAAGC AGTGATGGTG GGCCAAGAAA ACTACGACTG GGAGGTGTTC 
AAGAAGAACA CATTTTATGA AGGCGAATAC CATGATGAGC ATCCAAACAT ACTGGTGTTC 
TGGGAGGTGT TTGAGAGCCT GACAGCAGAG GAAAAGAAAA AGTTCCTTTT GTTTCTCACA 
GGCAGTGATC GCGTACCCTT CCTGGGAATG GAGAGCATCA AGATGAGAGT GGCTGTGCTG 
CCCGATGCTT CTGAACGCCA TTTGCCTGAA TCGCTTACCT GCCACTTCCT GTTGTTACTG 
CCCATCTACA GGAGATACCC AGTCGAGAGG ACGATGCGCA CAAGGCTTCT CCAGGCTATC 
AATCACAACA GAGGCTTCCG GAAGAAGCGA CACACCACAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005916012.1
CDS	59..3103
Translation	MQLFCWGDSSSGQFGSQAAPSPVSWTVPGVVTKICCGDRHTLFLNADGDVLSCGHNSQGQLGRQKKKNGKIIAQVEGLGRVVAMACGQDHSLAVSASGHVFSWGAGEDGQLGLPTPSNSHRPSKVPIPLPIPIIQVACGNSHSLALTKGGNVFSWGLNSHGQLGLGKEVPVQDTPVIICALAGVAVTQIAAGGTHSLFLTLADLVYCCGANKCGQLGVNRVDEKGRFNICMVPALRPLSVSFISCGESHSAVLTKDGKVYTFGDGSQGQLGHSTSANEVKPKLVAGVDGPASQIACGRHHTLVLGSSGQLWAFGSGAKGQTGNGHNGGSLTPTLVQFPWTTDDAAAMPSDLKIAAGWNTNFAYSSPAENSVHKQITGRLDETKLQEWLMMEHSNVEAKREIGSMFLTSSSLVASFTKANGFPLEAGDLTVDLEAASRAFDQLFTRQWIKQSVNLVPLCDLLYLSRTALKSPEIILILLTCPLLQEDSNVMNTVLSVAITIAEMSDKTLETLKKWWSSLSPSILMKHIMVFKKALAFMLKNGLLQTHNPGVKFILEALKLLYKANKAGKSYKVPLSTFYVEEITNAVQLDTDIMLWWQFSKEEDDVNTPAIFCRYPFLLTLLHKVAVFNFCAATVKNVHQLAHQAALVPHQPHAPDSPPAPVFQLTLRRSHLVEDTFRQLSAADHCAFQRELLVLFVEDRKTTNVNKRDFFLHVFDELMAPESKMFIYNESQTVAWFPPKPKVEEKTYFLFGVLCGLALYNHNIIHLRFPQALFKKLLRVKPTLDDMKEFDPVLAESWRIILEDYTPDVVQTLDTTFTVSWAGETVELDPNEPGKLVTGSNRKEYVAAFVNYVFNKSVERVFDEFKRGFFKVCNMDVVDFFQPEELQAVMVGQENYDWEVFKKNTFYEGEYHDEHPNILVFWEVFESLTAEEKKKFLLFLTGSDRVPFLGMESIKMRVAVLPDASERHLPESLTCHFLLLLPIYRRYPVERTMRTRLLQAINHNRGFRKKRHTTE

Target ORF information:

RefSeq Version	XM_005915950.1
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni probable E3 ubiquitin-protein ligase HERC6 (LOC102312366), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005915950.1

ORF Insert Sequence:

ATGCAGCTGT TCTGTTGGGG AGACAGCTCC AGCGGACAGT TCGGGTCACA GGCGGCCCCC 
AGCCCCGTGT CCTGGACCGT CCCCGGGGTT GTAACTAAGA TCTGCTGTGG GGATCGGCAC 
ACTTTATTTC TTAATGCAGA CGGGGACGTG CTGTCATGTG GGCACAACTC TCAGGGACAA 
CTTGGACGAC AGAAGAAAAA AAATGGAAAG ATCATAGCTC AAGTGGAGGG GCTGGGCCGT 
GTGGTGGCGA TGGCTTGCGG TCAGGACCAC TCTCTGGCTG TGAGTGCATC TGGACACGTT 
TTCTCCTGGG GGGCAGGAGA GGATGGACAG CTAGGGTTAC CAACACCTTC CAACAGTCAC 
AGACCAAGCA AGGTGCCTAT CCCACTGCCC ATACCGATAA TTCAAGTTGC TTGTGGAAAC 
TCCCATTCCC TTGCCCTGAC TAAAGGAGGC AATGTCTTCT CGTGGGGTTT AAACAGTCAT 
GGTCAGCTGG GCCTGGGGAA GGAGGTGCCG GTGCAGGATA CACCCGTCAT AATTTGTGCT 
TTGGCTGGAG TAGCGGTAAC CCAGATTGCA GCAGGAGGGA CCCACAGTCT GTTCCTTACC 
CTCGCAGACC TGGTGTACTG CTGTGGGGCC AATAAATGTG GTCAGCTCGG GGTTAACAGG 
GTGGATGAGA AAGGCAGGTT CAACATCTGT ATGGTGCCTG CGCTCAGACC TCTAAGTGTC 
TCCTTCATAA GCTGTGGAGA GTCGCACTCT GCTGTGCTGA CAAAGGACGG TAAAGTTTAC 
ACTTTTGGAG ACGGAAGCCA AGGTCAGCTC GGTCACAGCA CCTCTGCTAA TGAAGTGAAG 
CCCAAACTGG TTGCAGGTGT GGATGGTCCT GCTTCCCAGA TTGCATGTGG CAGGCATCAC 
ACTCTGGTTT TGGGCTCTTC TGGTCAGCTC TGGGCTTTTG GCAGTGGAGC TAAAGGTCAA 
ACTGGAAATG GGCACAATGG TGGCAGTCTG ACTCCCACTC TGGTTCAGTT TCCATGGACC 
ACTGACGATG CAGCAGCCAT GCCCAGTGAC TTGAAAATAG CTGCTGGGTG GAACACAAAC 
TTTGCTTACT CTTCACCTGC AGAGAACTCT GTTCACAAAC AGATAACTGG ACGTCTTGAT 
GAGACAAAAC TCCAAGAATG GCTGATGATG GAGCATAGTA ATGTGGAGGC AAAAAGAGAA 
ATCGGATCGA TGTTTCTAAC AAGTTCAAGT CTTGTTGCAA GTTTCACAAA AGCCAATGGG 
TTTCCACTGG AAGCAGGCGA CTTAACCGTG GACCTGGAGG CCGCTAGCCG AGCTTTCGAC 
CAGCTGTTCA CAAGACAGTG GATCAAACAA TCAGTAAACT TGGTCCCTCT GTGCGACTTG 
CTTTACCTGT CAAGAACTGC ATTAAAATCT CCAGAGATCA TCCTGATTCT TCTGACCTGC 
CCGCTTCTTC AAGAGGACTC TAATGTCATG AATACAGTTC TATCTGTGGC CATCACTATA 
GCAGAGATGT CGGACAAGAC TCTGGAAACA TTAAAAAAGT GGTGGAGTTC ACTGTCCCCC 
TCGATACTGA TGAAGCACAT CATGGTCTTC AAAAAGGCTC TCGCCTTCAT GCTAAAGAAT 
GGCCTCTTAC AAACACACAA CCCAGGAGTC AAATTTATCC TGGAAGCCCT CAAGCTGCTA 
TACAAAGCAA ACAAAGCTGG AAAGTCCTAC AAAGTCCCAC TGAGCACCTT TTATGTAGAA 
GAAATCACAA ATGCTGTGCA GCTTGACACT GACATCATGC TTTGGTGGCA ATTCTCCAAA 
GAGGAGGATG ACGTGAACAC ACCTGCCATC TTCTGTCGCT ACCCATTCCT TTTGACCCTG 
CTTCACAAAG TGGCCGTATT TAACTTCTGT GCAGCCACAG TGAAGAATGT GCATCAACTC 
GCTCATCAAG CAGCTTTGGT GCCCCATCAA CCGCACGCCC CAGACTCTCC ACCTGCCCCA 
GTCTTCCAGC TAACCCTGAG GCGATCTCAC CTGGTCGAGG ACACCTTCAG ACAGCTCAGT 
GCAGCCGACC ACTGTGCATT CCAAAGGGAG CTTTTAGTGC TGTTTGTGGA GGACAGGAAG 
ACAACAAATG TCAACAAAAG GGACTTTTTC CTGCACGTGT TTGATGAGCT GATGGCTCCA 
GAATCTAAAA TGTTCATATA TAATGAGAGC CAGACTGTGG CCTGGTTCCC TCCCAAGCCA 
AAAGTAGAGG AGAAGACTTA CTTCCTGTTT GGCGTTCTGT GTGGCCTGGC TCTCTACAAC 
CACAACATTA TCCACCTGCG CTTCCCACAA GCTCTGTTCA AGAAGCTGCT CAGGGTCAAA 
CCTACGCTGG ACGACATGAA GGAGTTTGAC CCTGTGCTGG CAGAGTCTTG GAGGATCATC 
TTAGAGGACT ACACCCCTGA TGTTGTTCAA ACGCTGGACA CTACTTTCAC TGTGTCCTGG 
GCTGGTGAGA CAGTTGAGCT TGATCCAAAT GAACCAGGAA AACTTGTCAC CGGGTCAAAC 
AGGAAAGAAT ACGTAGCTGC CTTTGTGAAC TACGTCTTCA ACAAATCAGT GGAGAGAGTG 
TTTGACGAGT TCAAGAGAGG GTTTTTCAAA GTGTGCAACA TGGACGTGGT GGACTTCTTC 
CAGCCAGAGG AGCTGCAAGC AGTGATGGTG GGCCAAGAAA ACTACGACTG GGAGGTGTTC 
AAGAAGAACA CATTTTATGA AGGCGAATAC CATGATGAGC ATCCAAACAT ACTGGTGTTC 
TGGGAGGTGT TTGAGAGCCT GACAGCAGAG GAAAAGAAAA AGTTCCTTTT GTTTCTCACA 
GGCAGTGATC GCGTACCCTT CCTGGGAATG GAGAGCATCA AGATGAGAGT GGCTGTGCTG 
CCCGATGCTT CTGAACGCCA TTTGCCTGAA TCGCTTACCT GCCACTTCCT GTTGTTACTG 
CCCATCTACA GGAGATACCC AGTCGAGAGG ACGATGCGCA CAAGGCTTCT CCAGGCTATC 
AATCACAACA GAGGCTTCCG GAAGAAGCGA CACACCACAG AGTGA