RASGRF2 cDNA ORF clone, Gekko japonicus

Gene
Clones
gRNAs

Summary

Gene Symbol	RASGRF2
Entrez Gene ID	107124620
Full Name	Ras protein specific guanine nucleotide releasing factor 2
Gene Type	protein-coding
Organism	Gekko japonicus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015428113.1	XP_015283599.1	ras-specific guanine nucleotide-releasing factor 2

Pathways from BioSystems
Gene Ontology

The following RASGRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe35511	XM_015428113.1 Latest version!	Gekko japonicus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGRF2 ( XM_015428113.1 ) cDNA ORF clone, Gekko japonicus -> XP_015283599.1 Gekko japonicus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), mRNA.

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CloneID	OGe35511
Clone ID Related Accession (Same CDS sequence)	XM_015428113.1
Accession Version	XM_015428113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	ras-specific guanine nucleotide-releasing factor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015177051.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAAA GCGTGCGCTA CAACGAGGGA CATGCCTTGT ACCTGGCCAT GCTGGCCCGC 
AAGGAAGGCA CCAAGCGCGG TTACCTGACC AAGAAGACGG CGGAGACGAG CCGCTGGCAT 
GAGAAGTGGT TCGCGCTCTA TCAGAACATG CTCTTCTACT TCGAGAGCGA GCAGAGCACC 
CGGCCCGCCG GCGTGTATAT GCTGGAAGGC TGCACCTGTG AGAGGACGCC CGCGCCCAAG 
GGTGCTTCCG CCGGCAGCGC CAAAGACGCG GCGTTGGACA AGCAGACATA TTTTACTGTC 
CTCTTTGGCC ATGAAGGGCA GAAACCACTT GAGCTCCGTT GTGACGAGGA AGTTGATGGA 
GACGAATGGG TAGAGGCTAT TCACCAAGCT AGCTACTCGG ATATTCTGAT TGAGAGGGAG 
GTATTGATGC AAAAATATAT CCATCTAGTT CAGATTGTAG AGACTGAAAA GATTTCAGCC 
AATCAGCTAA GACATCAGCT AGAAGATCAA GACACAGAAA TTGAAAGGCT TAAATCAGAG 
ATTGTAGCTC TCAATAAGAC GAAAGAACGA ATGCGACCTT ATCAAAATAA CCAAGAAGAA 
GAAGATCCTG ATATTAAAAA AATTAAAAAG GTTCAGAGTT TTATGCGAGG TTGGCTGTGT 
AGAAGAAAAT GGAAGACAAT TGTCCAAGAT TACATTTGCT CTCCACATGC AGAAAGTATG 
CGGAAGAGAA ACCAGATAGT TTTTAACATG GTCGAGGCTG AATCTGAGTA TGTGCATCAG 
CTTTACATCC TTGTGAATTG TTTCCTAAGA CCATTAAGGA TGGCTGCAAG CTCAAAGAAA 
CCACCAATTA GTCATGATGA TGTCAGCAGT ATATTCCTTA ACAGTGAAAC CATAATGTTT 
CTTCATGAAA TATTTCATCA AGGATTAAAA GCAAGAATAG CAAATTGGCC TACTTTAATT 
TTAGCTGATT TATTTGATAT CCTGCTACCT ATGCTGAATA TTTATCAAGA ATTCGTTCGG 
AATCACCAGT ATAGTCTTCA AGTTCTTGCA AACTGTAAGC AGAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAATCCTGCA TGTGAGGGAC GGATGCTTGA GACATTTCTG 
ACTTACCCTA TGTTTCAGAT TCCTAGATAC ATTATAACAT TACATGAACT TCTGGCTCAT 
ACACCACATG AACATGTGGA GAGAAAGAGT CTTGAATTTG CTAAATCAAA ACTAGAAGAA 
CTTTCAAGAG TGATGCATGA TGAAGTAAGT GACACAGAAA ACATACGGAA AAATCTTGCA 
ATAGAAAGAA TGATAGTGGA GGGTTGTGAT ATATTATTGG ACACAAGCCA GACTTTTATA 
CGACAAGGTA CTCTTATTCA GGTCCCTTCA GTTGAGAGGG GAAAACTTAG TAAAGTTCGT 
CTGGGATCAT TGTCTTTGAA AAAAGAAGGT GAAAGACAAT GTTTTTTATT TACAAAACAC 
TTTTTAATTT GTACAAGGAG CTCTGGAGGA AAGCTGCACC TGCTTAAGGC AGGAGGTGTT 
CTCTCGTTAA TAGAATGCAC ACTAATTGAG GAAACTGATA TGAATGATGA TGACTCAAAA 
GGTTCCGGGC AAGTGTTTGG ACATCTTGAT TTCAAGATCG TGGTAGAACC ACCTGATGCT 
CCTCCTTTCA CTGTTGTACT GTTAGCCCCT TCACGGCAGG AAAAAGCAGC GTGGACAAGT 
GACATTAGTC AGTGCATTGA CAATATACGG TGCAATGGTT TAATGACCAT AGTATTTGAA 
GAAAACTCCA AAGTAACTGT GCCACATATG ATCAAATCTG ATGCTCGCCT TCACAGAGAT 
GACGTTGACA TTTGCTTCAG CAAAACATTA AATTCCTGCA AAGTGCCCCA GATTCGTTAT 
GCAAGTGTAG AACGCCTTTT GGAGAGATTA ACCGACTTGC GATTTCTCAG TATTGATTTT 
CTTAATACTT TCCTACATAC TTATCGCATA TTTACTACTG CTGCAGTTGT ACTAGAGAAA 
CTTTCAGACA TATACAAAAG GCCTTTGAGC TCAATCCCTG TTCGCTCTTT GGAGTTGTTT 
TTTGCTACTA ATCAAAACAG CAGAGGAGAA CATCTGGCTG ATGCAAAATC ACCACATCTC 
TGCCGCAAAT TCTCTTCTCC TCCACCTCTG TCACTTTCTA GAACGTCCTC TCCTGTCACA 
ACCAGAAAAC TGTCATTAAG TTCTCCACTG AATTCACGAT TAGGAGCATT AGATCTATCC 
ACTTCTTCCC TTGCTAGTAG TCCTACCTCA ACATACAGCC CTGCCACATC ACCACCACCT 
ACATCTTCTA AAGTACCACT GGATCTTAGC AAAGGTCCTT CATCTCCAGA ACAAAGTCCA 
GGTTTCACAG AAGAAAATTC AGAGAATCCA CGTATTGAGC TTTGCAACAA ATTAAGGAGG 
AGCATTCGAA GAGCGGTTCT AGAGTCTGTG TCAGTGGACC GCACAATACC AGAAAGTGCC 
TCAACTGTGG ATACACCTGA ACTTTCGCCA TGTAGATCTC CTTCAACTCC ACGACATCTA 
CGTTATCGAC CACCAGGAGT ACAGACCACT GACAACAGTC ATTGTTCTGT TTCCCCTGCT 
TCTGCTTTTG CCATTGCTAC AGCTGCAGCT GGCCATGGAA GTCCACCAGG ATTTAATAAC 
AATGAACGAA CATGTGATAA AGAGTTCATA ATACGTCGAG CAGCCACCAA TCGTGTTCTG 
AATGTCCTTC GCCACTGGGT CTCAAAACAT TCTCAGGATT TTGAGCTAAA TAATGAACTG 
AAAATGAATG TTCTAAATCT GCTAGAAGAA GTTTTGAGAG ACCCTGACCT TTTGCCACAA 
GAAAGAAAGG CAACAGCAAA CATACTGAGG GCATTTTCTC AAGAGGATCA AGATGACCCT 
CACTTAAAAA TGGAGGATAT ATTGCAGATG TTAGAAAGTC CAAAACCAGA GTGCTTTGAA 
ACTTTATCAG CCATGGAGCT TGCTGAGCAA ATAACACTCT TGGATCACAT TGTTTTCAGA 
AGCATACCCT ACGAAGAGTT TCTTGGGCAG GGCTGGATGA AACTGGATAA AAATGAAAGG 
ACACCTTATA TTATGAAAAC TAGCCAACAC TTTAACGATA TGAGCAACCT TGTGGCCTCC 
CAAATTATGA ATTACGCGGA TGTTACCTCA CGTGCTAATG CAATTGAGAA ATGGGTAGCA 
GTGGCTGACA TCTGCCGATG TTTGCATAAC TACAACGGTG TGCTTGAAAT CACATCAGCC 
TTGAATAGAA GTGCAATCTA CAGACTGAAG AAAACATGGG CTAAGATTTC TAAACAGAGT 
AAAGCTCTTA TGGATAAGCT TCAGAAGACT GTTTCATCTG AGGGAAGATT TAAAAATCTC 
AGAGAAACAC TCAAGAATTG TAATCCCCCC TCAGTGCCAT ATCTTGGGAT GTACTTGACA 
GACTTGGCAT TTATTGAAGA AGGAACACCT AACTTTACTG AAGAAGGCCT TGTCAATTTT 
TCCAAAATGC GAATGATATC GCATATTATC AGGGAGGTAC GCCAGTTTCA GCAGACATCC 
TATCGCATAG AACATCAACA AAAGGTCACT CAGTACTTAC TTGACAAGAG TCTCATCATA 
GATGAAGATA CATTGTATGA GCTATCCCTA ACAATTGAAC CCAGACTGCC TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015283599.1
CDS	1..3717
Translation	MQKSVRYNEGHALYLAMLARKEGTKRGYLTKKTAETSRWHEKWFALYQNMLFYFESEQSTRPAGVYMLEGCTCERTPAPKGASAGSAKDAALDKQTYFTVLFGHEGQKPLELRCDEEVDGDEWVEAIHQASYSDILIEREVLMQKYIHLVQIVETEKISANQLRHQLEDQDTEIERLKSEIVALNKTKERMRPYQNNQEEEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFNMVEAESEYVHQLYILVNCFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGTLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKAGGVLSLIECTLIEETDMNDDDSKGSGQVFGHLDFKIVVEPPDAPPFTVVLLAPSRQEKAAWTSDISQCIDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHRDDVDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLEKLSDIYKRPLSSIPVRSLELFFATNQNSRGEHLADAKSPHLCRKFSSPPPLSLSRTSSPVTTRKLSLSSPLNSRLGALDLSTSSLASSPTSTYSPATSPPPTSSKVPLDLSKGPSSPEQSPGFTEENSENPRIELCNKLRRSIRRAVLESVSVDRTIPESASTVDTPELSPCRSPSTPRHLRYRPPGVQTTDNSHCSVSPASAFAIATAAAGHGSPPGFNNNERTCDKEFIIRRAATNRVLNVLRHWVSKHSQDFELNNELKMNVLNLLEEVLRDPDLLPQERKATANILRAFSQEDQDDPHLKMEDILQMLESPKPECFETLSAMELAEQITLLDHIVFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVTSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKISKQSKALMDKLQKTVSSEGRFKNLRETLKNCNPPSVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREVRQFQQTSYRIEHQQKVTQYLLDKSLIIDEDTLYELSLTIEPRLPA

Target ORF information:

RefSeq Version	XM_015428113.1
Organism	Gekko japonicus
Definition	Gekko japonicus Ras protein specific guanine nucleotide releasing factor 2 (RASGRF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015428113.1

ORF Insert Sequence:

ATGCAGAAAA GCGTGCGCTA CAACGAGGGA CATGCCTTGT ACCTGGCCAT GCTGGCCCGC 
AAGGAAGGCA CCAAGCGCGG TTACCTGACC AAGAAGACGG CGGAGACGAG CCGCTGGCAT 
GAGAAGTGGT TCGCGCTCTA TCAGAACATG CTCTTCTACT TCGAGAGCGA GCAGAGCACC 
CGGCCCGCCG GCGTGTATAT GCTGGAAGGC TGCACCTGTG AGAGGACGCC CGCGCCCAAG 
GGTGCTTCCG CCGGCAGCGC CAAAGACGCG GCGTTGGACA AGCAGACATA TTTTACTGTC 
CTCTTTGGCC ATGAAGGGCA GAAACCACTT GAGCTCCGTT GTGACGAGGA AGTTGATGGA 
GACGAATGGG TAGAGGCTAT TCACCAAGCT AGCTACTCGG ATATTCTGAT TGAGAGGGAG 
GTATTGATGC AAAAATATAT CCATCTAGTT CAGATTGTAG AGACTGAAAA GATTTCAGCC 
AATCAGCTAA GACATCAGCT AGAAGATCAA GACACAGAAA TTGAAAGGCT TAAATCAGAG 
ATTGTAGCTC TCAATAAGAC GAAAGAACGA ATGCGACCTT ATCAAAATAA CCAAGAAGAA 
GAAGATCCTG ATATTAAAAA AATTAAAAAG GTTCAGAGTT TTATGCGAGG TTGGCTGTGT 
AGAAGAAAAT GGAAGACAAT TGTCCAAGAT TACATTTGCT CTCCACATGC AGAAAGTATG 
CGGAAGAGAA ACCAGATAGT TTTTAACATG GTCGAGGCTG AATCTGAGTA TGTGCATCAG 
CTTTACATCC TTGTGAATTG TTTCCTAAGA CCATTAAGGA TGGCTGCAAG CTCAAAGAAA 
CCACCAATTA GTCATGATGA TGTCAGCAGT ATATTCCTTA ACAGTGAAAC CATAATGTTT 
CTTCATGAAA TATTTCATCA AGGATTAAAA GCAAGAATAG CAAATTGGCC TACTTTAATT 
TTAGCTGATT TATTTGATAT CCTGCTACCT ATGCTGAATA TTTATCAAGA ATTCGTTCGG 
AATCACCAGT ATAGTCTTCA AGTTCTTGCA AACTGTAAGC AGAACAGAGA TTTTGACAAA 
CTCTTGAAGC AATATGAAGC CAATCCTGCA TGTGAGGGAC GGATGCTTGA GACATTTCTG 
ACTTACCCTA TGTTTCAGAT TCCTAGATAC ATTATAACAT TACATGAACT TCTGGCTCAT 
ACACCACATG AACATGTGGA GAGAAAGAGT CTTGAATTTG CTAAATCAAA ACTAGAAGAA 
CTTTCAAGAG TGATGCATGA TGAAGTAAGT GACACAGAAA ACATACGGAA AAATCTTGCA 
ATAGAAAGAA TGATAGTGGA GGGTTGTGAT ATATTATTGG ACACAAGCCA GACTTTTATA 
CGACAAGGTA CTCTTATTCA GGTCCCTTCA GTTGAGAGGG GAAAACTTAG TAAAGTTCGT 
CTGGGATCAT TGTCTTTGAA AAAAGAAGGT GAAAGACAAT GTTTTTTATT TACAAAACAC 
TTTTTAATTT GTACAAGGAG CTCTGGAGGA AAGCTGCACC TGCTTAAGGC AGGAGGTGTT 
CTCTCGTTAA TAGAATGCAC ACTAATTGAG GAAACTGATA TGAATGATGA TGACTCAAAA 
GGTTCCGGGC AAGTGTTTGG ACATCTTGAT TTCAAGATCG TGGTAGAACC ACCTGATGCT 
CCTCCTTTCA CTGTTGTACT GTTAGCCCCT TCACGGCAGG AAAAAGCAGC GTGGACAAGT 
GACATTAGTC AGTGCATTGA CAATATACGG TGCAATGGTT TAATGACCAT AGTATTTGAA 
GAAAACTCCA AAGTAACTGT GCCACATATG ATCAAATCTG ATGCTCGCCT TCACAGAGAT 
GACGTTGACA TTTGCTTCAG CAAAACATTA AATTCCTGCA AAGTGCCCCA GATTCGTTAT 
GCAAGTGTAG AACGCCTTTT GGAGAGATTA ACCGACTTGC GATTTCTCAG TATTGATTTT 
CTTAATACTT TCCTACATAC TTATCGCATA TTTACTACTG CTGCAGTTGT ACTAGAGAAA 
CTTTCAGACA TATACAAAAG GCCTTTGAGC TCAATCCCTG TTCGCTCTTT GGAGTTGTTT 
TTTGCTACTA ATCAAAACAG CAGAGGAGAA CATCTGGCTG ATGCAAAATC ACCACATCTC 
TGCCGCAAAT TCTCTTCTCC TCCACCTCTG TCACTTTCTA GAACGTCCTC TCCTGTCACA 
ACCAGAAAAC TGTCATTAAG TTCTCCACTG AATTCACGAT TAGGAGCATT AGATCTATCC 
ACTTCTTCCC TTGCTAGTAG TCCTACCTCA ACATACAGCC CTGCCACATC ACCACCACCT 
ACATCTTCTA AAGTACCACT GGATCTTAGC AAAGGTCCTT CATCTCCAGA ACAAAGTCCA 
GGTTTCACAG AAGAAAATTC AGAGAATCCA CGTATTGAGC TTTGCAACAA ATTAAGGAGG 
AGCATTCGAA GAGCGGTTCT AGAGTCTGTG TCAGTGGACC GCACAATACC AGAAAGTGCC 
TCAACTGTGG ATACACCTGA ACTTTCGCCA TGTAGATCTC CTTCAACTCC ACGACATCTA 
CGTTATCGAC CACCAGGAGT ACAGACCACT GACAACAGTC ATTGTTCTGT TTCCCCTGCT 
TCTGCTTTTG CCATTGCTAC AGCTGCAGCT GGCCATGGAA GTCCACCAGG ATTTAATAAC 
AATGAACGAA CATGTGATAA AGAGTTCATA ATACGTCGAG CAGCCACCAA TCGTGTTCTG 
AATGTCCTTC GCCACTGGGT CTCAAAACAT TCTCAGGATT TTGAGCTAAA TAATGAACTG 
AAAATGAATG TTCTAAATCT GCTAGAAGAA GTTTTGAGAG ACCCTGACCT TTTGCCACAA 
GAAAGAAAGG CAACAGCAAA CATACTGAGG GCATTTTCTC AAGAGGATCA AGATGACCCT 
CACTTAAAAA TGGAGGATAT ATTGCAGATG TTAGAAAGTC CAAAACCAGA GTGCTTTGAA 
ACTTTATCAG CCATGGAGCT TGCTGAGCAA ATAACACTCT TGGATCACAT TGTTTTCAGA 
AGCATACCCT ACGAAGAGTT TCTTGGGCAG GGCTGGATGA AACTGGATAA AAATGAAAGG 
ACACCTTATA TTATGAAAAC TAGCCAACAC TTTAACGATA TGAGCAACCT TGTGGCCTCC 
CAAATTATGA ATTACGCGGA TGTTACCTCA CGTGCTAATG CAATTGAGAA ATGGGTAGCA 
GTGGCTGACA TCTGCCGATG TTTGCATAAC TACAACGGTG TGCTTGAAAT CACATCAGCC 
TTGAATAGAA GTGCAATCTA CAGACTGAAG AAAACATGGG CTAAGATTTC TAAACAGAGT 
AAAGCTCTTA TGGATAAGCT TCAGAAGACT GTTTCATCTG AGGGAAGATT TAAAAATCTC 
AGAGAAACAC TCAAGAATTG TAATCCCCCC TCAGTGCCAT ATCTTGGGAT GTACTTGACA 
GACTTGGCAT TTATTGAAGA AGGAACACCT AACTTTACTG AAGAAGGCCT TGTCAATTTT 
TCCAAAATGC GAATGATATC GCATATTATC AGGGAGGTAC GCCAGTTTCA GCAGACATCC 
TATCGCATAG AACATCAACA AAAGGTCACT CAGTACTTAC TTGACAAGAG TCTCATCATA 
GATGAAGATA CATTGTATGA GCTATCCCTA ACAATTGAAC CCAGACTGCC TGCTTGA