NELFB cDNA ORF clone, Gekko japonicus

Gene
Clones
gRNAs

Summary

Gene Symbol	NELFB
Entrez Gene ID	107118786
Full Name	negative elongation factor complex member B
Gene Type	protein-coding
Organism	Gekko japonicus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015421151.1	XP_015276637.1	negative elongation factor B

Pathways from BioSystems
Gene Ontology

The following NELFB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NELFB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe30178	XM_015421151.1 Latest version!	Gekko japonicus negative elongation factor complex member B (NELFB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NELFB ( XM_015421151.1 ) cDNA ORF clone, Gekko japonicus -> XP_015276637.1 Gekko japonicus negative elongation factor complex member B (NELFB), mRNA.

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CloneID	OGe30178
Clone ID Related Accession (Same CDS sequence)	XM_015421151.1
Accession Version	XM_015421151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	negative elongation factor B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015172554.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTTCGCTG GGCTGCAGGA GCTCGGGGTG GCTAATGGGG AGGACTTAAA GGAGACGCTG 
ACCAACTGCA CAGAGCCGCT CAAGGCCATC GAGCAGTTCC AGACAGAAAA TGGCATCCTG 
CTGCCCTCTC TCCAGTCTGC TCTGCCATTC CTGGACCTCC ATGGCACACC CCGGCTGGAG 
TTCCACCAGT CTGTGTTTGA TGAGCTGCGA GAGAAGTTGC TGGAAAGAGT CTCTGCTATT 
GCCTCCGAAG GAAAGGCTGA AGAAAGGTAC AAAAAGCTGG AAGATCTCCT GGAGAAGAGC 
TTCCCCTTGG TCAAGATGCC ATCCATACAG CCCGTGGTGA TGTGCGTCAT GAAACATCTG 
CCCAAGGTTC CTGAGAAGAA GCTGAAGCTT GTGATGGCGG ACAAGGATCT GTACAAAGCG 
TGCGCCATGG AAGTGAAGCG CCAGATCTGG CAGGATAACC AGGCCCTGTT TGGCGATGAG 
GTGTCCCCCT TACTGAAGCA GTACATCCTG GAGAAGGAGA ACACTTTGTT CAGCAGCGAC 
CTCTCTGTCC TGCACAACTT CTTCAGCCCG TCCCCCAAAA TGAGGCGCCA GGGAGAGGTG 
GTTCAGAAGC TCACGCAGAT GATTGGGAAG AATGTGAAGC TCTACGACAT GGTCCTGCAG 
TTCCTGAGGA CCTTGTTCCT CCGCACACGG AATGTCCATT ACTGCACATT GCGGGCCGAG 
CTCTTGATGT CGCTGCACGA CTTGGACATC AGTGAGATCT GTACAGTGGA CCCCTGCCAC 
AAGTTTACCT GGTGCTTGGA TGCCTGCATT CGAGAGAAGT TTGTAGACAA CAAGCGTGCT 
CGAGAGCTTC AGGGGTTCCT AGATGGAGTG AAGAAAGGAC AAGAACAAGT CCTCGGGGAT 
CTATCCATGA TCTTGTGTGA CCCGTTCGCC ATCAACACCT TAGCGCTGAG CACCATCCGT 
CACTTGCAAG AGCTGATTGG GCAGGACTTA TTACCCCGGG AAAGCCCCGA CCTTCTGCTT 
CTGTTGAGAA TGCTGTCGCT GGGGCAGGGA GCTTGGGACA TGATCGACAG CCAGATCTTC 
AAGGAACCAA AAATGGAAGT CGACCTGATC ACCAAATTCT TGCCAACTCT CATGTCTTTT 
GGGGTGGATG ACTACACGTT CACCGTTGAT CAGAAGCTGC CGTCAGAGGA CAAGGGCCCC 
CCGCCATACC CCAGTGCCAT CCCGGAAATG TTTCCAAACA TAACTAAGCA GAGGAATAAG 
AATGCTCTTC TCCGGTTGCT CCCATCACTA GCAGAGACCT TCAACGACCT GGCCTTCAGC 
GACATTTTCC TGCACCTGTT CACCGGCAAC CTCACCCTCC TTGCTGACGA ATTTGCGCAA 
GAAGAGTTCT GTACCAGTCT CTTTGACAAC TTCTTCCTTG CTGCCTGCAC AAGGAAAGAG 
AACGTACAGA AGCACGTGCT GCGGCTGCTG CTTCACCTGC ATCACAAAGT AGCTCCTGCC 
CAGTTGGAGA CCTTGCAGAA GTCGTTGGAG CCCACCAAAC AGAGTGGCGA AGCTGTGAAG 
GAGCTGCACA GCCAGCTCAG CGAGAAACTG GAACTCCGCA AGCCCAGCCC AGCCCAGGTG 
GCTGACGCCC CGTCCATGGA CTTGCCTCTA CCGACAGTCC CCACCCCAGC TCCCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015276637.1
CDS	49..1728
Translation	MFAGLQELGVANGEDLKETLTNCTEPLKAIEQFQTENGILLPSLQSALPFLDLHGTPRLEFHQSVFDELREKLLERVSAIASEGKAEERYKKLEDLLEKSFPLVKMPSIQPVVMCVMKHLPKVPEKKLKLVMADKDLYKACAMEVKRQIWQDNQALFGDEVSPLLKQYILEKENTLFSSDLSVLHNFFSPSPKMRRQGEVVQKLTQMIGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDISEICTVDPCHKFTWCLDACIREKFVDNKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLALSTIRHLQELIGQDLLPRESPDLLLLLRMLSLGQGAWDMIDSQIFKEPKMEVDLITKFLPTLMSFGVDDYTFTVDQKLPSEDKGPPPYPSAIPEMFPNITKQRNKNALLRLLPSLAETFNDLAFSDIFLHLFTGNLTLLADEFAQEEFCTSLFDNFFLAACTRKENVQKHVLRLLLHLHHKVAPAQLETLQKSLEPTKQSGEAVKELHSQLSEKLELRKPSPAQVADAPSMDLPLPTVPTPAPL

Target ORF information:

RefSeq Version	XM_015421151.1
Organism	Gekko japonicus
Definition	Gekko japonicus negative elongation factor complex member B (NELFB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015421151.1

ORF Insert Sequence:

ATGTTCGCTG GGCTGCAGGA GCTCGGGGTG GCTAATGGGG AGGACTTAAA GGAGACGCTG 
ACCAACTGCA CAGAGCCGCT CAAGGCCATC GAGCAGTTCC AGACAGAAAA TGGCATCCTG 
CTGCCCTCTC TCCAGTCTGC TCTGCCATTC CTGGACCTCC ATGGCACACC CCGGCTGGAG 
TTCCACCAGT CTGTGTTTGA TGAGCTGCGA GAGAAGTTGC TGGAAAGAGT CTCTGCTATT 
GCCTCCGAAG GAAAGGCTGA AGAAAGGTAC AAAAAGCTGG AAGATCTCCT GGAGAAGAGC 
TTCCCCTTGG TCAAGATGCC ATCCATACAG CCCGTGGTGA TGTGCGTCAT GAAACATCTG 
CCCAAGGTTC CTGAGAAGAA GCTGAAGCTT GTGATGGCGG ACAAGGATCT GTACAAAGCG 
TGCGCCATGG AAGTGAAGCG CCAGATCTGG CAGGATAACC AGGCCCTGTT TGGCGATGAG 
GTGTCCCCCT TACTGAAGCA GTACATCCTG GAGAAGGAGA ACACTTTGTT CAGCAGCGAC 
CTCTCTGTCC TGCACAACTT CTTCAGCCCG TCCCCCAAAA TGAGGCGCCA GGGAGAGGTG 
GTTCAGAAGC TCACGCAGAT GATTGGGAAG AATGTGAAGC TCTACGACAT GGTCCTGCAG 
TTCCTGAGGA CCTTGTTCCT CCGCACACGG AATGTCCATT ACTGCACATT GCGGGCCGAG 
CTCTTGATGT CGCTGCACGA CTTGGACATC AGTGAGATCT GTACAGTGGA CCCCTGCCAC 
AAGTTTACCT GGTGCTTGGA TGCCTGCATT CGAGAGAAGT TTGTAGACAA CAAGCGTGCT 
CGAGAGCTTC AGGGGTTCCT AGATGGAGTG AAGAAAGGAC AAGAACAAGT CCTCGGGGAT 
CTATCCATGA TCTTGTGTGA CCCGTTCGCC ATCAACACCT TAGCGCTGAG CACCATCCGT 
CACTTGCAAG AGCTGATTGG GCAGGACTTA TTACCCCGGG AAAGCCCCGA CCTTCTGCTT 
CTGTTGAGAA TGCTGTCGCT GGGGCAGGGA GCTTGGGACA TGATCGACAG CCAGATCTTC 
AAGGAACCAA AAATGGAAGT CGACCTGATC ACCAAATTCT TGCCAACTCT CATGTCTTTT 
GGGGTGGATG ACTACACGTT CACCGTTGAT CAGAAGCTGC CGTCAGAGGA CAAGGGCCCC 
CCGCCATACC CCAGTGCCAT CCCGGAAATG TTTCCAAACA TAACTAAGCA GAGGAATAAG 
AATGCTCTTC TCCGGTTGCT CCCATCACTA GCAGAGACCT TCAACGACCT GGCCTTCAGC 
GACATTTTCC TGCACCTGTT CACCGGCAAC CTCACCCTCC TTGCTGACGA ATTTGCGCAA 
GAAGAGTTCT GTACCAGTCT CTTTGACAAC TTCTTCCTTG CTGCCTGCAC AAGGAAAGAG 
AACGTACAGA AGCACGTGCT GCGGCTGCTG CTTCACCTGC ATCACAAAGT AGCTCCTGCC 
CAGTTGGAGA CCTTGCAGAA GTCGTTGGAG CCCACCAAAC AGAGTGGCGA AGCTGTGAAG 
GAGCTGCACA GCCAGCTCAG CGAGAAACTG GAACTCCGCA AGCCCAGCCC AGCCCAGGTG 
GCTGACGCCC CGTCCATGGA CTTGCCTCTA CCGACAGTCC CCACCCCAGC TCCCCTCTAG