RASEF cDNA ORF clone, Gekko japonicus

Gene
Clones
gRNAs

Summary

Gene Symbol	RASEF
Entrez Gene ID	107117202
Full Name	RAS and EF-hand domain containing
Gene Type	protein-coding
Organism	Gekko japonicus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015419277.1	XP_015274763.1	ras and EF-hand domain-containing protein

Pathways from BioSystems
Gene Ontology

The following RASEF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASEF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe28732	XM_015419277.1 Latest version!	Gekko japonicus RAS and EF-hand domain containing (RASEF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASEF ( XM_015419277.1 ) cDNA ORF clone, Gekko japonicus -> XP_015274763.1 Gekko japonicus RAS and EF-hand domain containing (RASEF), mRNA.

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CloneID	OGe28732
Clone ID Related Accession (Same CDS sequence)	XM_015419277.1
Accession Version	XM_015419277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	ras and EF-hand domain-containing protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015171506.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAG AGGGGCGCGA GCAGGACGGG AGCGACGCCT TCGACGCCTT GGCTGGGGTG 
GCGGCCGCGG CCACTGCGGC GGGGCCGAGC CAGGCTTGGC GAGAATTCGA GCTGCGGCTC 
GGCGAGGAAG CGGGCTACAT CCCCAGGCAA GAGCAGGTCA GTGTTCTGTA TCAGAACATC 
AGTATAGTGG AACCACGATT AATTCAGCCT TATGAACATG TAATTAAGAA CTTCATTCGT 
GAGATCAAGC TTCAGAGTGC GGAGATGGAA AACTTAGCCA TAGCTGTAAA AAGAGCACAG 
GATAAAGCGG CAATGCAGCT GAGTGAACTG GAAGAGGAGA TGGACCAGCG AATACAAGCT 
GCAGAGCATA AAATCAAGAA AGAGGAGAAA CGCAAGGCAG AAGAGGCTCT CGGTGATCTC 
AAGCAGCAGT ATGAAGCTGA AGTCGATGAC CTCCAAGTGA CAATAAAGAA ACTTAAAATG 
CTTGAAGAAC AATCTAAAAA CATCAATCAC AAAGGAGATT TGGCACTACT AAGGAACAGG 
ATACGTGATT TGACATTGGA AAATCATAAA CTTAAAAAAG ATCTCATGGA AGCACAAACA 
AATATTGCTT TCCTTCAGAG TGAATTAGAT ACTTTGAAAA GTGACCTTGC TGATCAGAGT 
TTGAATTCAG AGAGAGACCT GGAAATGATC AGAGAATACA CTGAAGACAG AGATAGCTTG 
GAAAGGCAGA TAGAAATACT GCAGACAGCG AACAGGAAAC TTCATGACAG TAACGATGGT 
TTACGAAGTG CATTAGAGAA CAGCTGGAGC ATATATAACA GATCGCTGCG TCTAACCAAC 
TCGTCTCCAG CAAACACAGT CTCCAGAAAC AGTCCTAAAT GCATCGGTGG CCAGTCTCCT 
TCCTATGTGG ATGAAGACTG TGATTCTCTG GCACTTTGTG ATCCTATGCA GAGGATAAAC 
TGCGAGGTAG ACAGCTTGCC TGAAAGCTGC TTTGACAGTG GTCTATCTAC CTTGAGAGAC 
TCCAATGAAT ATGATTCAGA ACTGGAATCC AAACATCAAA GGATTTCTCA AAGGTCACAG 
TGTCCACAGG AAAGCTTTGC GGGAGATGCT TCTGATACAG ACGTCCCTGA TATCAGAGAT 
GAAGAAGTGT ATAGTCCTGA TGAAGTCAGT ACGGTGTTAG ATTGGAAACC ATGCCAGCCT 
ATTAATTGCA GCTCGAGGTA CCCAAGCTCA GAGAAGGCTT ACAAGATAGT TCTTGCTGGG 
GATGCAGCAG TTGGGAAATC TAGTTTCCTT TTGAGACTTT GCAAAAATGA ATTTCGAGGG 
AACACCAGTG CAACTCTAGG GGTTGATTTT CAGATGAAGA GGCTTATTGT TGATGGTGAA 
CCTACAGTAC TACAGCTGTG GGACACAGCT GGACAAGAGA GGTTCCGGAG CATCGCAAAA 
TCATATTTCA GAAGAGCAGA TGGCGTGCTG CTGCTATATG ATGTTACGTG TGAGAAAAGT 
TTTCTGAATG TACGAGAATG GATAGATATG ATTGAGGATG CAACACATGA AAATATCCCA 
ATCATGGTGG TCGGAAACAA AGCAGACCTT CGTCCACTGG CTTATGAACA AGGAGATAAA 
TGTGTACCAG TAAATTATGG AGAAAAGTTG GCCATGGCTT ACAATGCATT GTTCTGTGAA 
ACAAGTGCCA AAGATGGTTC TAATATTGTG GAGGCAGTTC TTCACCTTGC TCGAGAAGTG 
CGGAAAAGAA GCAATAGTGA AGATGACAAC AGGTCTGTAA CCGACTTGGC AGGGACAACA 
ACAAAGAGGT CAACGCTCAC CAAAAATTGC TGCAGTGTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015274763.1
CDS	34..1875
Translation	MEGEGREQDGSDAFDALAGVAAAATAAGPSQAWREFELRLGEEAGYIPRQEQVSVLYQNISIVEPRLIQPYEHVIKNFIREIKLQSAEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKIKKEEKRKAEEALGDLKQQYEAEVDDLQVTIKKLKMLEEQSKNINHKGDLALLRNRIRDLTLENHKLKKDLMEAQTNIAFLQSELDTLKSDLADQSLNSERDLEMIREYTEDRDSLERQIEILQTANRKLHDSNDGLRSALENSWSIYNRSLRLTNSSPANTVSRNSPKCIGGQSPSYVDEDCDSLALCDPMQRINCEVDSLPESCFDSGLSTLRDSNEYDSELESKHQRISQRSQCPQESFAGDASDTDVPDIRDEEVYSPDEVSTVLDWKPCQPINCSSRYPSSEKAYKIVLAGDAAVGKSSFLLRLCKNEFRGNTSATLGVDFQMKRLIVDGEPTVLQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWIDMIEDATHENIPIMVVGNKADLRPLAYEQGDKCVPVNYGEKLAMAYNALFCETSAKDGSNIVEAVLHLAREVRKRSNSEDDNRSVTDLAGTTTKRSTLTKNCCSV

Target ORF information:

RefSeq Version	XM_015419277.1
Organism	Gekko japonicus
Definition	Gekko japonicus RAS and EF-hand domain containing (RASEF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015419277.1

ORF Insert Sequence:

ATGGAAGGAG AGGGGCGCGA GCAGGACGGG AGCGACGCCT TCGACGCCTT GGCTGGGGTG 
GCGGCCGCGG CCACTGCGGC GGGGCCGAGC CAGGCTTGGC GAGAATTCGA GCTGCGGCTC 
GGCGAGGAAG CGGGCTACAT CCCCAGGCAA GAGCAGGTCA GTGTTCTGTA TCAGAACATC 
AGTATAGTGG AACCACGATT AATTCAGCCT TATGAACATG TAATTAAGAA CTTCATTCGT 
GAGATCAAGC TTCAGAGTGC GGAGATGGAA AACTTAGCCA TAGCTGTAAA AAGAGCACAG 
GATAAAGCGG CAATGCAGCT GAGTGAACTG GAAGAGGAGA TGGACCAGCG AATACAAGCT 
GCAGAGCATA AAATCAAGAA AGAGGAGAAA CGCAAGGCAG AAGAGGCTCT CGGTGATCTC 
AAGCAGCAGT ATGAAGCTGA AGTCGATGAC CTCCAAGTGA CAATAAAGAA ACTTAAAATG 
CTTGAAGAAC AATCTAAAAA CATCAATCAC AAAGGAGATT TGGCACTACT AAGGAACAGG 
ATACGTGATT TGACATTGGA AAATCATAAA CTTAAAAAAG ATCTCATGGA AGCACAAACA 
AATATTGCTT TCCTTCAGAG TGAATTAGAT ACTTTGAAAA GTGACCTTGC TGATCAGAGT 
TTGAATTCAG AGAGAGACCT GGAAATGATC AGAGAATACA CTGAAGACAG AGATAGCTTG 
GAAAGGCAGA TAGAAATACT GCAGACAGCG AACAGGAAAC TTCATGACAG TAACGATGGT 
TTACGAAGTG CATTAGAGAA CAGCTGGAGC ATATATAACA GATCGCTGCG TCTAACCAAC 
TCGTCTCCAG CAAACACAGT CTCCAGAAAC AGTCCTAAAT GCATCGGTGG CCAGTCTCCT 
TCCTATGTGG ATGAAGACTG TGATTCTCTG GCACTTTGTG ATCCTATGCA GAGGATAAAC 
TGCGAGGTAG ACAGCTTGCC TGAAAGCTGC TTTGACAGTG GTCTATCTAC CTTGAGAGAC 
TCCAATGAAT ATGATTCAGA ACTGGAATCC AAACATCAAA GGATTTCTCA AAGGTCACAG 
TGTCCACAGG AAAGCTTTGC GGGAGATGCT TCTGATACAG ACGTCCCTGA TATCAGAGAT 
GAAGAAGTGT ATAGTCCTGA TGAAGTCAGT ACGGTGTTAG ATTGGAAACC ATGCCAGCCT 
ATTAATTGCA GCTCGAGGTA CCCAAGCTCA GAGAAGGCTT ACAAGATAGT TCTTGCTGGG 
GATGCAGCAG TTGGGAAATC TAGTTTCCTT TTGAGACTTT GCAAAAATGA ATTTCGAGGG 
AACACCAGTG CAACTCTAGG GGTTGATTTT CAGATGAAGA GGCTTATTGT TGATGGTGAA 
CCTACAGTAC TACAGCTGTG GGACACAGCT GGACAAGAGA GGTTCCGGAG CATCGCAAAA 
TCATATTTCA GAAGAGCAGA TGGCGTGCTG CTGCTATATG ATGTTACGTG TGAGAAAAGT 
TTTCTGAATG TACGAGAATG GATAGATATG ATTGAGGATG CAACACATGA AAATATCCCA 
ATCATGGTGG TCGGAAACAA AGCAGACCTT CGTCCACTGG CTTATGAACA AGGAGATAAA 
TGTGTACCAG TAAATTATGG AGAAAAGTTG GCCATGGCTT ACAATGCATT GTTCTGTGAA 
ACAAGTGCCA AAGATGGTTC TAATATTGTG GAGGCAGTTC TTCACCTTGC TCGAGAAGTG 
CGGAAAAGAA GCAATAGTGA AGATGACAAC AGGTCTGTAA CCGACTTGGC AGGGACAACA 
ACAAAGAGGT CAACGCTCAC CAAAAATTGC TGCAGTGTTT AG