WT1 cDNA ORF clone, Gekko japonicus

Gene
Clones
gRNAs

Summary

Gene Symbol	WT1
Entrez Gene ID	107109060
Full Name	Wilms tumor 1
Gene Type	protein-coding
Organism	Gekko japonicus

mRNA and Protein(s)

mRNA	Protein	Name
XM_015409637.1	XP_015265123.1	Wilms tumor protein

Pathways from BioSystems
Gene Ontology

The following WT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGe15991	XM_015409637.1 Latest version!	Gekko japonicus Wilms tumor 1 (WT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WT1 ( XM_015409637.1 ) cDNA ORF clone, Gekko japonicus -> XP_015265123.1 Gekko japonicus Wilms tumor 1 (WT1), mRNA.

Price & Availability↑

CloneID	OGe15991
Clone ID Related Accession (Same CDS sequence)	XM_015409637.1
Accession Version	XM_015409637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-19
Organism	Gekko japonicus
Product	Wilms tumor protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015160211.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gekko japonicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 25% of CDS bases ##RefSeq-Attributes-END##

ATGTATCCGT TACCAATAAC GGCACTACTA ATGTCCACCT CGTCTTTTGA GGATGAGAAG 
CAGCACGGCG AGAGGGTCGG CGTTCTCGAC CCTTTGCCGC GAATGGTGGA CTCGGGCAAG 
TGTCTGCGGA GAGACTGTCG ACGTCTCTTT CTCTCAACGG GCACGTGGCC ACCCCGTAAG 
GCAATCCTCT GTGGCGAGGA GAAGTTTCAC ACACTTCTGA GCCTCCTCAG CCACGCCGAG 
AAGTTCAGGC GCAGAGGCGG GGGTGTGCCG AGAAGTCTAA GCACAGAGGC GGCGGCAGCA 
GCGGAGGTGG GGGCGCGCAT CTTCCCCAGC GGGACCTACC TGCCCAGCTG CCTGGAGAGC 
CAGCAAGCCA TCCGCAACCA AGGTTACGGC ACAGTGGCGT TTGATGGAAC TCCAAGTTAT 
GGACATACTC CCTCTCACCA TGCAGCACAG TTTACAAACC ACTCTTTTAA ACATGAGGAC 
CCCATCAGCC AACAGACATC TCTAGGTGAC CAACAGTATT CGGTGCCTCC CCCAGTCTAT 
GGCTGCCACA CTCCCACAGA TAGCCAGGCC TTACTCCTGA GGACCCCATA CAACAGCAGT 
GACAATTTAT ACCAAATGAC TTCACAGCTT GAATGCATGA CATGGAATCA AATGAACTTG 
GGATCTGCAC TTAAGGGCCA CGCAGCAGGA TATGAAAGTG ACAGCCATGC AGGTCCTATG 
CTATACAGCT GTGGGGCTCA ATATAGAATA CATACTCATG GAGTCTTTAG AGGAATACAA 
GATATGCGGA GAGTGCCAGG AGTAACTCCT ACAATTGTTA GATCTGCAAG TGAAACAAAT 
GAGAAAAGGC CTTTCATGTG TACATACCCT GGCTGTAACA AGCGATACTT TAAATTGTCT 
CACCTGCATA TGCACAGCAG AAAACACACT GGTGAGAAAC CATACCAGTG TGACTTTAAG 
GATTGTGAAA GAAGGTTCTC TCGTTCAGAT CAACTCAAAC GACACCAAAG ACGACACACA 
GGTGTGAAAC CCTTCCAGTG TAAAACTTGT CAGAGAAAGT TTTCCAGATC TGATCACCTG 
AAGACTCACA CCAGGACTCA TACAGGTAAA ACAAGTGAAA AGCCTTTCAG TTGCCGATGG 
CCCAGCTGTC AAAAAAAATT TGCCCGATCT GATGAATTGG TCCGTCATCA TAATATGCAC 
AACAGGAACA TGACTAAACT TCAGCTTACC CTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015265123.1
CDS	1..1236
Translation	MYPLPITALLMSTSSFEDEKQHGERVGVLDPLPRMVDSGKCLRRDCRRLFLSTGTWPPRKAILCGEEKFHTLLSLLSHAEKFRRRGGGVPRSLSTEAAAAAEVGARIFPSGTYLPSCLESQQAIRNQGYGTVAFDGTPSYGHTPSHHAAQFTNHSFKHEDPISQQTSLGDQQYSVPPPVYGCHTPTDSQALLLRTPYNSSDNLYQMTSQLECMTWNQMNLGSALKGHAAGYESDSHAGPMLYSCGAQYRIHTHGVFRGIQDMRRVPGVTPTIVRSASETNEKRPFMCTYPGCNKRYFKLSHLHMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHNRNMTKLQLTL

Target ORF information:

RefSeq Version	XM_015409637.1
Organism	Gekko japonicus
Definition	Gekko japonicus Wilms tumor 1 (WT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015409637.1

ORF Insert Sequence:

ATGTATCCGT TACCAATAAC GGCACTACTA ATGTCCACCT CGTCTTTTGA GGATGAGAAG 
CAGCACGGCG AGAGGGTCGG CGTTCTCGAC CCTTTGCCGC GAATGGTGGA CTCGGGCAAG 
TGTCTGCGGA GAGACTGTCG ACGTCTCTTT CTCTCAACGG GCACGTGGCC ACCCCGTAAG 
GCAATCCTCT GTGGCGAGGA GAAGTTTCAC ACACTTCTGA GCCTCCTCAG CCACGCCGAG 
AAGTTCAGGC GCAGAGGCGG GGGTGTGCCG AGAAGTCTAA GCACAGAGGC GGCGGCAGCA 
GCGGAGGTGG GGGCGCGCAT CTTCCCCAGC GGGACCTACC TGCCCAGCTG CCTGGAGAGC 
CAGCAAGCCA TCCGCAACCA AGGTTACGGC ACAGTGGCGT TTGATGGAAC TCCAAGTTAT 
GGACATACTC CCTCTCACCA TGCAGCACAG TTTACAAACC ACTCTTTTAA ACATGAGGAC 
CCCATCAGCC AACAGACATC TCTAGGTGAC CAACAGTATT CGGTGCCTCC CCCAGTCTAT 
GGCTGCCACA CTCCCACAGA TAGCCAGGCC TTACTCCTGA GGACCCCATA CAACAGCAGT 
GACAATTTAT ACCAAATGAC TTCACAGCTT GAATGCATGA CATGGAATCA AATGAACTTG 
GGATCTGCAC TTAAGGGCCA CGCAGCAGGA TATGAAAGTG ACAGCCATGC AGGTCCTATG 
CTATACAGCT GTGGGGCTCA ATATAGAATA CATACTCATG GAGTCTTTAG AGGAATACAA 
GATATGCGGA GAGTGCCAGG AGTAACTCCT ACAATTGTTA GATCTGCAAG TGAAACAAAT 
GAGAAAAGGC CTTTCATGTG TACATACCCT GGCTGTAACA AGCGATACTT TAAATTGTCT 
CACCTGCATA TGCACAGCAG AAAACACACT GGTGAGAAAC CATACCAGTG TGACTTTAAG 
GATTGTGAAA GAAGGTTCTC TCGTTCAGAT CAACTCAAAC GACACCAAAG ACGACACACA 
GGTGTGAAAC CCTTCCAGTG TAAAACTTGT CAGAGAAAGT TTTCCAGATC TGATCACCTG 
AAGACTCACA CCAGGACTCA TACAGGTAAA ACAAGTGAAA AGCCTTTCAG TTGCCGATGG 
CCCAGCTGTC AAAAAAAATT TGCCCGATCT GATGAATTGG TCCGTCATCA TAATATGCAC 
AACAGGAACA TGACTAAACT TCAGCTTACC CTTTAG