PCK1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCK1
Entrez Gene ID	396458
Full Name	phosphoenolpyruvate carboxykinase 1
Synonyms	PEPCK
General protein information	Preferred Names phosphoenolpyruvate carboxykinase 1 Names phosphoenolpyruvate carboxykinase, cytosolic [GTP] PEPCK-C cytosolic phosphoenolpyruvate carboxykinase phosphoenolpyruvate carboxykinase 1 (soluble) phosphoenolpyruvate carboxykinase, cytosolic phosphoenolpyruvate carboxylase
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205471.1	NP_990802.1	phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PCK1	NP_777162.1
	PCK1	XP_001086710.1
Mus musculus (house mouse)	Pck1	NP_035174.1
Danio rerio (zebrafish)	pck1	NP_999916.1
Xenopus tropicalis (tropical clawed frog)	pck1	NP_001073036.1
Rattus norvegicus (Norway rat)	Pck1	NP_942075.1
Gallus gallus (chicken)	PCK1	NP_990802.1
Homo sapiens (human)	PCK1	NP_002582.3
Pan troglodytes (chimpanzee)	PCK1	XP_514745.2
Canis lupus familiaris (dog)	PCK1	NP_001184072.1
Drosophila melanogaster (fruit fly)	CG10924	NP_611341.1

Gene Ontology
Bibliography

Related articles in PubMed

Breed-dependent transcriptional regulation of phosphoenolpyruvate carboxylase, cytosolic form, expression in the liver of broiler chickens.
Guo F, Zhang Y, Su L, Ahmed AA, Ni Y, Zhao R
Poultry science92(10)2737-44(2013 Oct)

Aryl hydrocarbon receptor activation by dioxin targets phosphoenolpyruvate carboxykinase (PEPCK) for ADP-ribosylation via 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible poly(ADP-ribose) polymerase (TiPARP).
Diani-Moore S, Zhang S, Ram P, Rifkind AB
The Journal of biological chemistry288(30)21514-25(2013 Jul)

Androgen inhibits abdominal fat accumulation and negatively regulates the PCK1 gene in male chickens.
Duan J, Shao F, Shao Y, Li J, Ling Y, Teng K, Li H, Wu C
PloS one8(3)e59636(2013)

Restriction fragment length and single strand conformational polymorphisms in chicken mitochondrial phosphoenol-pyruate carboxykinase gene and its association with egg production.
Torkamanzehi A, Kuhnlein U
Pakistan journal of biological sciences : PJBS10(22)4075-80(2007 Nov)

Alleles of cytosolic phosphoenolpyruvate carboxykinase (PEPCK): trait association and interaction with mitochondrial PEPCK in a strain of White Leghorn chickens.
Parsanejad R, Torkamanzehi A, Zadworny D, Kuhnlein U
Poultry science82(11)1708-15(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A fast-growing chicken breed had significantly higher hepatic mRNA expression levels of the cytosolic form of PEPCK and significantly higher hepatic mRNA and protein expression levels of cAMP response element binding protein 1.
Title: Breed-dependent transcriptional regulation of phosphoenolpyruvate carboxylase, cytosolic form, expression in the liver of broiler chickens.

Data indicate that aryl hydrocarbon receptor (AHR) enhances phosphoenolpyruvate carboxykinase (PEPCK) ADP-ribosylation through TiPARP TiPARP (TCDD-inducible poly(ADP-ribose) polymerase).
Title: Aryl hydrocarbon receptor activation by dioxin targets phosphoenolpyruvate carboxykinase (PEPCK) for ADP-ribosylation via 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-inducible poly(ADP-ribose) polymerase (TiPARP).

androgen status negatively regulated the PCK1 gene in male chickens.
Title: Androgen inhibits abdominal fat accumulation and negatively regulates the PCK1 gene in male chickens.

analysis of restriction fragment length and single strand conformational polymorphisms in chicken mitochondrial phosphoenol-pyruate carboxykinase gene and its association with egg production
Title: Restriction fragment length and single strand conformational polymorphisms in chicken mitochondrial phosphoenol-pyruate carboxykinase gene and its association with egg production.

The following PCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28029	NM_205471.1 Latest version!	Gallus gallus phosphoenolpyruvate carboxykinase 1 (PCK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCK1 ( NM_205471.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990802.1 Gallus gallus phosphoenolpyruvate carboxykinase 1 (PCK1), mRNA.

Price & Availability↑

CloneID	OGa28029
Clone ID Related Accession (Same CDS sequence)	NM_205471.1
Accession Version	NM_205471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Gallus gallus(chicken)
Product	phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M14229.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M14229.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCCCAG AGCTGAAAAC TGAGGTCAAC ATCATCTCTA AGGTTATCCA GGGGGACTTG 
GAGAGCCTGC CTCCACAAGT GAGGGAGTTC ATTGAAAGCA ATGCCAAGCT GTGCCAGCCT 
GAGAGCATTC ATATCTGCGA TGGCTCAGAA GAAGAAAACA AAAAAATTCT GGACATCATG 
GTGGAGCAAG GCATGATCAA GAAGCTGAGC AAGTATGAGA ACTGCTGGTT GGCTCTCACT 
AACCCAAGAG ACGTAGCAAG AATCGAGAGC AAAACCGTCA TTATTACTCA AGAACAGAGA 
GATACCATTC CAATCCCTAA AACTGGAAGT AGCCAGCTGG GTCGCTGGAT GTCAGAAGAG 
GATTTTGAGA AAGCTTTCAA CACCAGATTC CCAGGCTGCA TGCAAGGACG CACAATGTAT 
GTCATCCCCT TCAGCATGGG GCCGATTGGA TCTCCTTTGG CCAAGATTGG GATTGAGCTG 
ACAGATTCTC CATATGTGGT GGCTAGCATG AGGATGATGA CACGGATGGG AACAGCTGCT 
CTGAAAGCGC TGGGCAATGG AGAGTTTGTG AAATGCCTTC ACTCGGTTGG ATGTCCTCTA 
CCACTAAAAG AACCATTAAT CAACAACTGG CCATGCAACC CAGAGTTAAC ACTGATTGCT 
CATCTCCCGG ATCGCAGAGA GATCATTTCA TTTGGCAGCG GCTACGGAGG AAACTCCTTA 
CTGGGGAAAA AATGCTTTGC TCTTCGAATT GCCAGCAGAA TCGCCAAGGA AGAGGGCTGG 
CTAGCAGAGC ACATGCTGAT CCTGGGAATT ACAAATCCAG AAGGTGAAAA GAAGTATTTT 
GCTGCAGCAT TCCCCAGTGC ATGTGGAAAA ACCAACTTGG CCATGATGAA CCCAAGCCGT 
CCAGGATGGA AGATTGAGTG TGTGGGTGAT GATATTGCCT GGATGAAATT TGATGAACTG 
GGCAACTTAA GGGCAATCAA TCCAGAAAAC GGCTTTTTTG GTGTTGCCCC TGGAACATCA 
ATTAAAACAA ACCCCAATGC TATTAAAACC ATATTTAAGA ACACCATCTT TACCAATGTA 
GCTGAAACAA GTGATGGGGG TGTCTATTGG GAAGGCATTG ATGAGCCACT ACCACCGGGA 
GTAACGCTGA CTTCATGGAA GAATAAGGAT TGGACTCCAG ATAATGGGGA GCCGTGTGCT 
CATCCCAACT CGCGGTTCTG CACTCCAGCC AGCCAGTGCC CCATCATGGA CCCTGCATGG 
GAGTCGCCTG AAGGTGTGCC CATTGAGGGC ATAATATTTG GAGGTCGCAG ACCTGCTGGT 
GTGCCTCTTG TATACGAGGC CTTTAACTGG CAGCATGGAG TATTTATAGG AGCAGCTATG 
AGATCTGAAG CAACAGCAGC TGCCGAGCAC AAAGGAAAAA TTATTATGCA TGATCCATTT 
GCCATGAGAC CATTTTTTGG CTACAATTTT GGCAAATACT TAGCACACTG GCTTAGCATG 
GCACACCGCC CAGCAGCAAA ACTACCAAGG ATCTTTCATG TTAATTGGTT CCGGAAAGAC 
AGCCAAGGGA AATTCCTGTG GCCTGGCTAT GGAGAGAATT CCCGTGTGCT GGAGTGGATG 
TTCAACAGAA TTCAAGGGAA AGCCTCTGCC AAGTCAACTG CCATAGGTTA CATCCCTGCT 
GACACTGCTT TGAACTTGAA GGGTTTAGAA GACATCAACT TAACTGAACT GTTTAATATC 
TCCAAAGAGT TCTGGGAAAA GGAGGTGGAA GAAATCAAAC AGTACTTTGA AGGGCAAGTT 
AACGCTGACC TTCCCTATGA AATAGAAAGA GAGCTGCTTG CCTTGGAGAT GAGAATAAAA 
CAGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990802.1
CDS	247..2115
Translation	MAPELKTEVNIISKVIQGDLESLPPQVREFIESNAKLCQPESIHICDGSEEENKKILDIMVEQGMIKKLSKYENCWLALTNPRDVARIESKTVIITQEQRDTIPIPKTGSSQLGRWMSEEDFEKAFNTRFPGCMQGRTMYVIPFSMGPIGSPLAKIGIELTDSPYVVASMRMMTRMGTAALKALGNGEFVKCLHSVGCPLPLKEPLINNWPCNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRIAKEEGWLAEHMLILGITNPEGEKKYFAAAFPSACGKTNLAMMNPSRPGWKIECVGDDIAWMKFDELGNLRAINPENGFFGVAPGTSIKTNPNAIKTIFKNTIFTNVAETSDGGVYWEGIDEPLPPGVTLTSWKNKDWTPDNGEPCAHPNSRFCTPASQCPIMDPAWESPEGVPIEGIIFGGRRPAGVPLVYEAFNWQHGVFIGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPAAKLPRIFHVNWFRKDSQGKFLWPGYGENSRVLEWMFNRIQGKASAKSTAIGYIPADTALNLKGLEDINLTELFNISKEFWEKEVEEIKQYFEGQVNADLPYEIERELLALEMRIKQL

Target ORF information:

RefSeq Version	NM_205471.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phosphoenolpyruvate carboxykinase 1 (PCK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205471.1

ORF Insert Sequence:

ATGGCCCCAG AGCTGAAAAC TGAGGTCAAC ATCATCTCTA AGGTTATCCA GGGGGACTTG 
GAGAGCCTGC CTCCACAAGT GAGGGAGTTC ATTGAAAGCA ATGCCAAGCT GTGCCAGCCT 
GAGAGCATTC ATATCTGCGA TGGCTCAGAA GAAGAAAACA AAAAAATTCT GGACATCATG 
GTGGAGCAAG GCATGATCAA GAAGCTGAGC AAGTATGAGA ACTGCTGGTT GGCTCTCACT 
AACCCAAGAG ACGTAGCAAG AATCGAGAGC AAAACCGTCA TTATTACTCA AGAACAGAGA 
GATACCATTC CAATCCCTAA AACTGGAAGT AGCCAGCTGG GTCGCTGGAT GTCAGAAGAG 
GATTTTGAGA AAGCTTTCAA CACCAGATTC CCAGGCTGCA TGCAAGGACG CACAATGTAT 
GTCATCCCCT TCAGCATGGG GCCGATTGGA TCTCCTTTGG CCAAGATTGG GATTGAGCTG 
ACAGATTCTC CATATGTGGT GGCTAGCATG AGGATGATGA CACGGATGGG AACAGCTGCT 
CTGAAAGCGC TGGGCAATGG AGAGTTTGTG AAATGCCTTC ACTCGGTTGG ATGTCCTCTA 
CCACTAAAAG AACCATTAAT CAACAACTGG CCATGCAACC CAGAGTTAAC ACTGATTGCT 
CATCTCCCGG ATCGCAGAGA GATCATTTCA TTTGGCAGCG GCTACGGAGG AAACTCCTTA 
CTGGGGAAAA AATGCTTTGC TCTTCGAATT GCCAGCAGAA TCGCCAAGGA AGAGGGCTGG 
CTAGCAGAGC ACATGCTGAT CCTGGGAATT ACAAATCCAG AAGGTGAAAA GAAGTATTTT 
GCTGCAGCAT TCCCCAGTGC ATGTGGAAAA ACCAACTTGG CCATGATGAA CCCAAGCCGT 
CCAGGATGGA AGATTGAGTG TGTGGGTGAT GATATTGCCT GGATGAAATT TGATGAACTG 
GGCAACTTAA GGGCAATCAA TCCAGAAAAC GGCTTTTTTG GTGTTGCCCC TGGAACATCA 
ATTAAAACAA ACCCCAATGC TATTAAAACC ATATTTAAGA ACACCATCTT TACCAATGTA 
GCTGAAACAA GTGATGGGGG TGTCTATTGG GAAGGCATTG ATGAGCCACT ACCACCGGGA 
GTAACGCTGA CTTCATGGAA GAATAAGGAT TGGACTCCAG ATAATGGGGA GCCGTGTGCT 
CATCCCAACT CGCGGTTCTG CACTCCAGCC AGCCAGTGCC CCATCATGGA CCCTGCATGG 
GAGTCGCCTG AAGGTGTGCC CATTGAGGGC ATAATATTTG GAGGTCGCAG ACCTGCTGGT 
GTGCCTCTTG TATACGAGGC CTTTAACTGG CAGCATGGAG TATTTATAGG AGCAGCTATG 
AGATCTGAAG CAACAGCAGC TGCCGAGCAC AAAGGAAAAA TTATTATGCA TGATCCATTT 
GCCATGAGAC CATTTTTTGG CTACAATTTT GGCAAATACT TAGCACACTG GCTTAGCATG 
GCACACCGCC CAGCAGCAAA ACTACCAAGG ATCTTTCATG TTAATTGGTT CCGGAAAGAC 
AGCCAAGGGA AATTCCTGTG GCCTGGCTAT GGAGAGAATT CCCGTGTGCT GGAGTGGATG 
TTCAACAGAA TTCAAGGGAA AGCCTCTGCC AAGTCAACTG CCATAGGTTA CATCCCTGCT 
GACACTGCTT TGAACTTGAA GGGTTTAGAA GACATCAACT TAACTGAACT GTTTAATATC 
TCCAAAGAGT TCTGGGAAAA GGAGGTGGAA GAAATCAAAC AGTACTTTGA AGGGCAAGTT 
AACGCTGACC TTCCCTATGA AATAGAAAGA GAGCTGCTTG CCTTGGAGAT GAGAATAAAA 
CAGTTGTAG