E2F1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	E2F1
Entrez Gene ID	396142
Full Name	E2F transcription factor 1
General protein information	Preferred Names E2F transcription factor 1 Names transcription factor E2F1 E2F-1 transcription factor
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015296338.1	XP_015151824.1	transcription factor E2F1 isoform X1
NM_205219.1	NP_990550.1	transcription factor E2F1
XM_015296338.2	XP_015151824.1	transcription factor E2F1 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC101885420	XP_005174590.1
	E2F1	XP_001103717.2
Pan troglodytes (chimpanzee)	E2F1	XP_003316910.1
Homo sapiens (human)	E2F1	NP_005216.1
Rattus norvegicus (Norway rat)	E2f1	NP_001094248.1
Xenopus tropicalis (tropical clawed frog)	e2f1	XP_002932903.1
Canis lupus familiaris (dog)	E2F1	XP_005635002.1
Bos taurus (cattle)	E2F1	NP_001193008.1
Mus musculus (house mouse)	E2f1	NP_031917.1
Gallus gallus (chicken)	E2F1	NP_990550.1

Gene Ontology
Bibliography

Related articles in PubMed

E2F1-miR-20a-5p/20b-5p auto-regulatory feedback loop involved in myoblast proliferation and differentiation.
Luo W, Li G, Yi Z, Nie Q, Zhang X
Scientific reports627904(2016 Jun)

Isolation and characterization of a chicken homolog of the E2F-1 transcription factor.
Pasteau S, Loiseau L, Arnaud L, Trembleau A, Brun G
Oncogene11(8)1475-86(1995 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following E2F1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E2F1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa27713	NM_205219.1 Latest version!	Gallus gallus E2F transcription factor 1 (E2F1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OGa44699	XM_015296338.2 Latest version!	Gallus gallus E2F transcription factor 1 (E2F1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
View Old Accession Versions >>
OGa44699	XM_015296338.1	Gallus gallus E2F transcription factor 1 (E2F1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E2F1 ( NM_205219.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990550.1 Gallus gallus E2F transcription factor 1 (E2F1), mRNA.

Price & Availability↑

CloneID	OGa27713
Clone ID Related Accession (Same CDS sequence)	NM_205219.1
Accession Version	NM_205219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-14
Organism	Gallus gallus(chicken)
Product	transcription factor E2F1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X89245.1. ##Evidence-Data-START## Transcript exon combination :: X89245.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTACGG CGGGCGGCGC GGCGGGGCTG GCGGCGCTGC TGGGCGGCGC CTCCCCGCAC 
CTCCTCATCG TCTCGGCCTC CGAGGAGCCC GCGGGCGGCT GCCGCCCCGA CGCCGACCTC 
CTGCTCTTCG CCACGCCGCA GCCCTCCCGC CCCGGCCCCG CGCCGCGCCG CCCCGCGCTG 
GGCCGCCCGC CGGTGAAGCG GAAGCTGAAC TTGGAGACGG ATCACCAGTA CATAGCCGAG 
AGCCTGCCCG CGGCCCGCGG CAGGGCCCGG ATCCCCGGCA GAGGGGCAAA GTCTCCTGGG 
GAGAAGTCCC GCTATGAAAC CTCGCTGAAC CTCACCACCA AGCGCTTCCT GGAGCTGCTC 
AGCCAATCCC CCGACGGTGT GGTGGACCTC AACTGGGCAG CCGAGGTGCT GAAGGTGCAG 
AAGAGGCGCA TCTACGACAT CACCAATGTC CTGGAGGGCA TCCAGCTCAT CACCAAGAAG 
TCCAAGAACA ACATCCAGTG GCTGGGCAGC CAGGTGGCCG CGGGGGCCTC CAGCCGACAG 
CGGCTGCTGG AGAAGGAGCT GCGGGATCTG CAGGCGGCCG AGCGGCAGCT GGACGATCTC 
ATCCAGACGT GCACGGTCAG GCTGCGGCTG CTCACTGAGG ATCCCTCCAA CCAGCACGCA 
GCCTACGTCA CCTGCCAGGA CCTCCGGAGC ATCGTGGACC CCTCTGAGCA AATGGTGATG 
GTTATCAAGG CACCCCCAGA GACCCAGCTG CAAGTCTCGG ACCCGGGAGA GGCTTTCCAG 
GTCTCCGTGA GAAGCACTCA GGGCCCCATC GACGTGTTCC TCTGTCCCGA GGACAGCTCA 
GGGGTCTGCA GCCCCGTCAA GAGCCCCTTC AAGGCACCTG CTGAGGAGCT CTCCCCCGGC 
TCATCGCAGC AGAGAGCCTC CCCGCTCCTG CATTCTGCCC AGGATGTGAA CATGCTGCTG 
CCTGAGGCAC TGCTCCCAGG CACAGCGCTG CCGACCAAGT GCCCCACAGA GGACGTGAGC 
CTGTCGCCGC TAGCCTCCAT GGACACACTC CTGGAGCATG GCAAGGACGA TTTCCCGGGC 
TTCCTGGCGG ATGAGTTCAT TGCACTGTCA CCACCACAGC CACAGGATTA CCACTTCGGC 
CTGGAGGAGG GTGAGGGCAT CAGTGAGCTC TTTGACTGTG ACTTTGGGGA CTTCACGCAC 
TTGGACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990550.1
CDS	131..1342
Translation	MATAGGAAGLAALLGGASPHLLIVSASEEPAGGCRPDADLLLFATPQPSRPGPAPRRPALGRPPVKRKLNLETDHQYIAESLPAARGRARIPGRGAKSPGEKSRYETSLNLTTKRFLELLSQSPDGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLITKKSKNNIQWLGSQVAAGASSRQRLLEKELRDLQAAERQLDDLIQTCTVRLRLLTEDPSNQHAAYVTCQDLRSIVDPSEQMVMVIKAPPETQLQVSDPGEAFQVSVRSTQGPIDVFLCPEDSSGVCSPVKSPFKAPAEELSPGSSQQRASPLLHSAQDVNMLLPEALLPGTALPTKCPTEDVSLSPLASMDTLLEHGKDDFPGFLADEFIALSPPQPQDYHFGLEEGEGISELFDCDFGDFTHLDF

Target ORF information:

RefSeq Version	NM_205219.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus E2F transcription factor 1 (E2F1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205219.1

ORF Insert Sequence:

ATGGCTACGG CGGGCGGCGC GGCGGGGCTG GCGGCGCTGC TGGGCGGCGC CTCCCCGCAC 
CTCCTCATCG TCTCGGCCTC CGAGGAGCCC GCGGGCGGCT GCCGCCCCGA CGCCGACCTC 
CTGCTCTTCG CCACGCCGCA GCCCTCCCGC CCCGGCCCCG CGCCGCGCCG CCCCGCGCTG 
GGCCGCCCGC CGGTGAAGCG GAAGCTGAAC TTGGAGACGG ATCACCAGTA CATAGCCGAG 
AGCCTGCCCG CGGCCCGCGG CAGGGCCCGG ATCCCCGGCA GAGGGGCAAA GTCTCCTGGG 
GAGAAGTCCC GCTATGAAAC CTCGCTGAAC CTCACCACCA AGCGCTTCCT GGAGCTGCTC 
AGCCAATCCC CCGACGGTGT GGTGGACCTC AACTGGGCAG CCGAGGTGCT GAAGGTGCAG 
AAGAGGCGCA TCTACGACAT CACCAATGTC CTGGAGGGCA TCCAGCTCAT CACCAAGAAG 
TCCAAGAACA ACATCCAGTG GCTGGGCAGC CAGGTGGCCG CGGGGGCCTC CAGCCGACAG 
CGGCTGCTGG AGAAGGAGCT GCGGGATCTG CAGGCGGCCG AGCGGCAGCT GGACGATCTC 
ATCCAGACGT GCACGGTCAG GCTGCGGCTG CTCACTGAGG ATCCCTCCAA CCAGCACGCA 
GCCTACGTCA CCTGCCAGGA CCTCCGGAGC ATCGTGGACC CCTCTGAGCA AATGGTGATG 
GTTATCAAGG CACCCCCAGA GACCCAGCTG CAAGTCTCGG ACCCGGGAGA GGCTTTCCAG 
GTCTCCGTGA GAAGCACTCA GGGCCCCATC GACGTGTTCC TCTGTCCCGA GGACAGCTCA 
GGGGTCTGCA GCCCCGTCAA GAGCCCCTTC AAGGCACCTG CTGAGGAGCT CTCCCCCGGC 
TCATCGCAGC AGAGAGCCTC CCCGCTCCTG CATTCTGCCC AGGATGTGAA CATGCTGCTG 
CCTGAGGCAC TGCTCCCAGG CACAGCGCTG CCGACCAAGT GCCCCACAGA GGACGTGAGC 
CTGTCGCCGC TAGCCTCCAT GGACACACTC CTGGAGCATG GCAAGGACGA TTTCCCGGGC 
TTCCTGGCGG ATGAGTTCAT TGCACTGTCA CCACCACAGC CACAGGATTA CCACTTCGGC 
CTGGAGGAGG GTGAGGGCAT CAGTGAGCTC TTTGACTGTG ACTTTGGGGA CTTCACGCAC 
TTGGACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E2F1 ( XM_015296338.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151824.1 Gallus gallus E2F transcription factor 1 (E2F1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa44699
Clone ID Related Accession (Same CDS sequence)	XM_015296338.1 , XM_015296338.2
Accession Version	XM_015296338.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	transcription factor E2F1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455993.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACGG CGGGCGGCGC GGCGGGGCTG GCGGCGCTGC TGGGCGGCGC CTCCCCGCAC 
CTCCTCATCG TCTCGGCCTC CGAGGAGCCC GCGGGCGGCT GCCGCCCCGA CGCCGACCTC 
CTGCTCTTCG CCACGCCGCA GCCCTCCCGC CCCGGCCCCG CGCCGCGCCG CCCCGCGCTG 
GGCCGCCCGC CGGTGAAGCG GAAGCTGAAC TTGGAGACGG ATCACCAGTA CATAGCCGAG 
AGCCTGCCCG CGGCCCGCGG CAGGGCCCGG ATCCCCGGCA GAGGGGCAAA GTCTCCTGGG 
GAGAAGTCCC GCTATGAAAC CTCGCTGAAC CTCACCACCA AGCGCTTCCT GGAGCTGCTC 
AGCCAATCCC CCGACGGTGT GGTGGACCTC AACTGGGCGG CCGAGGTGCT GAAGGTGCAG 
AAGAGGCGCA TCTACGACAT CACCAATGTC CTGGAGGGCA TCCAGCTCAT CACCAAGAAG 
TCCAAGAACA ACATCCAGTG GCTGGGCAGC CAGGTGGCCG CGGGGGCCTC CAGCCGACAG 
CGGCTGCTGG AGAAGGAGCT GCGGGATCTG CAGGCGGCCG AGCGGCAGCT GGACGATCTC 
ATCCAGACGT GCACGGTGCA GCTGCGGCTG CTCACTGAGG ATCCCTCCAA CCAGCACGCA 
GCCTACGTCA CCTGCCAGGA CCTCCGGAGC ATCGTGGACC CCTCTGAGCA AATGGTGATG 
GTTATCAAGG CACCCCCAGA GACCCAGCTG CAAGTCTCGG ACCCGGGAGA GGCTTTCCAG 
GTCTCCGTGA GAAGCACTCA GGGCCCCATC GACGTGTTCC TCTGTCCCGA GGACAGCTCA 
GGGGTCTGCA GCCCCGTCAA GAGCCCCTTC AAGGCACCTG CTGAGGAGCT CTCCCCCGGC 
TCATCGCAGC AGAGAGCCTC CCCGCTCCTG CATTCTGCCC AGGATGTGAA CATGCTGCTG 
CCTGGTGAGC AAGAGGCACT GCTCCCAGGC ACAGCGCTGC CGACCAAGTG CCCCACAGAG 
GACGTGAGCC TGTCGCCGCT AGCCTCCATG GACACACTCC TGGAGCATGG CAAGGACGAT 
TTCCCGGGCT TCCTGGCGGA TGAGTTCATT GCACTGTCAC CACCACAGCC ACAGGATTAC 
CACTTCGGCC TGGAGGAGGG TGAGGGCATC AGTGAGCTCT TTGACTGTGA CTTTGGGGAC 
TTCACGCACT TGGACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151824.1
CDS	93..1313
Translation	MATAGGAAGLAALLGGASPHLLIVSASEEPAGGCRPDADLLLFATPQPSRPGPAPRRPALGRPPVKRKLNLETDHQYIAESLPAARGRARIPGRGAKSPGEKSRYETSLNLTTKRFLELLSQSPDGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLITKKSKNNIQWLGSQVAAGASSRQRLLEKELRDLQAAERQLDDLIQTCTVQLRLLTEDPSNQHAAYVTCQDLRSIVDPSEQMVMVIKAPPETQLQVSDPGEAFQVSVRSTQGPIDVFLCPEDSSGVCSPVKSPFKAPAEELSPGSSQQRASPLLHSAQDVNMLLPGEQEALLPGTALPTKCPTEDVSLSPLASMDTLLEHGKDDFPGFLADEFIALSPPQPQDYHFGLEEGEGISELFDCDFGDFTHLDF

Target ORF information:

RefSeq Version	XM_015296338.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus E2F transcription factor 1 (E2F1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015296338.1

ORF Insert Sequence:

ATGGCTACGG CGGGCGGCGC GGCGGGGCTG GCGGCGCTGC TGGGCGGCGC CTCCCCGCAC 
CTCCTCATCG TCTCGGCCTC CGAGGAGCCC GCGGGCGGCT GCCGCCCCGA CGCCGACCTC 
CTGCTCTTCG CCACGCCGCA GCCCTCCCGC CCCGGCCCCG CGCCGCGCCG CCCCGCGCTG 
GGCCGCCCGC CGGTGAAGCG GAAGCTGAAC TTGGAGACGG ATCACCAGTA CATAGCCGAG 
AGCCTGCCCG CGGCCCGCGG CAGGGCCCGG ATCCCCGGCA GAGGGGCAAA GTCTCCTGGG 
GAGAAGTCCC GCTATGAAAC CTCGCTGAAC CTCACCACCA AGCGCTTCCT GGAGCTGCTC 
AGCCAATCCC CCGACGGTGT GGTGGACCTC AACTGGGCGG CCGAGGTGCT GAAGGTGCAG 
AAGAGGCGCA TCTACGACAT CACCAATGTC CTGGAGGGCA TCCAGCTCAT CACCAAGAAG 
TCCAAGAACA ACATCCAGTG GCTGGGCAGC CAGGTGGCCG CGGGGGCCTC CAGCCGACAG 
CGGCTGCTGG AGAAGGAGCT GCGGGATCTG CAGGCGGCCG AGCGGCAGCT GGACGATCTC 
ATCCAGACGT GCACGGTGCA GCTGCGGCTG CTCACTGAGG ATCCCTCCAA CCAGCACGCA 
GCCTACGTCA CCTGCCAGGA CCTCCGGAGC ATCGTGGACC CCTCTGAGCA AATGGTGATG 
GTTATCAAGG CACCCCCAGA GACCCAGCTG CAAGTCTCGG ACCCGGGAGA GGCTTTCCAG 
GTCTCCGTGA GAAGCACTCA GGGCCCCATC GACGTGTTCC TCTGTCCCGA GGACAGCTCA 
GGGGTCTGCA GCCCCGTCAA GAGCCCCTTC AAGGCACCTG CTGAGGAGCT CTCCCCCGGC 
TCATCGCAGC AGAGAGCCTC CCCGCTCCTG CATTCTGCCC AGGATGTGAA CATGCTGCTG 
CCTGGTGAGC AAGAGGCACT GCTCCCAGGC ACAGCGCTGC CGACCAAGTG CCCCACAGAG 
GACGTGAGCC TGTCGCCGCT AGCCTCCATG GACACACTCC TGGAGCATGG CAAGGACGAT 
TTCCCGGGCT TCCTGGCGGA TGAGTTCATT GCACTGTCAC CACCACAGCC ACAGGATTAC 
CACTTCGGCC TGGAGGAGGG TGAGGGCATC AGTGAGCTCT TTGACTGTGA CTTTGGGGAC 
TTCACGCACT TGGACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E2F1 ( XM_015296338.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015151824.1 Gallus gallus E2F transcription factor 1 (E2F1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa44699
Clone ID Related Accession (Same CDS sequence)	XM_015296338.1 , XM_015296338.2
Accession Version	XM_015296338.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	transcription factor E2F1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006107.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015296338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACGG CGGGCGGCGC GGCGGGGCTG GCGGCGCTGC TGGGCGGCGC CTCCCCGCAC 
CTCCTCATCG TCTCGGCCTC CGAGGAGCCC GCGGGCGGCT GCCGCCCCGA CGCCGACCTC 
CTGCTCTTCG CCACGCCGCA GCCCTCCCGC CCCGGCCCCG CGCCGCGCCG CCCCGCGCTG 
GGCCGCCCGC CGGTGAAGCG GAAGCTGAAC TTGGAGACGG ATCACCAGTA CATAGCCGAG 
AGCCTGCCCG CGGCCCGCGG CAGGGCCCGG ATCCCCGGCA GAGGGGCAAA GTCTCCTGGG 
GAGAAGTCCC GCTATGAAAC CTCGCTGAAC CTCACCACCA AGCGCTTCCT GGAGCTGCTC 
AGCCAATCCC CCGACGGTGT GGTGGACCTC AACTGGGCGG CCGAGGTGCT GAAGGTGCAG 
AAGAGGCGCA TCTACGACAT CACCAATGTC CTGGAGGGCA TCCAGCTCAT CACCAAGAAG 
TCCAAGAACA ACATCCAGTG GCTGGGCAGC CAGGTGGCCG CGGGGGCCTC CAGCCGACAG 
CGGCTGCTGG AGAAGGAGCT GCGGGATCTG CAGGCGGCCG AGCGGCAGCT GGACGATCTC 
ATCCAGACGT GCACGGTGCA GCTGCGGCTG CTCACTGAGG ATCCCTCCAA CCAGCACGCA 
GCCTACGTCA CCTGCCAGGA CCTCCGGAGC ATCGTGGACC CCTCTGAGCA AATGGTGATG 
GTTATCAAGG CACCCCCAGA GACCCAGCTG CAAGTCTCGG ACCCGGGAGA GGCTTTCCAG 
GTCTCCGTGA GAAGCACTCA GGGCCCCATC GACGTGTTCC TCTGTCCCGA GGACAGCTCA 
GGGGTCTGCA GCCCCGTCAA GAGCCCCTTC AAGGCACCTG CTGAGGAGCT CTCCCCCGGC 
TCATCGCAGC AGAGAGCCTC CCCGCTCCTG CATTCTGCCC AGGATGTGAA CATGCTGCTG 
CCTGGTGAGC AAGAGGCACT GCTCCCAGGC ACAGCGCTGC CGACCAAGTG CCCCACAGAG 
GACGTGAGCC TGTCGCCGCT AGCCTCCATG GACACACTCC TGGAGCATGG CAAGGACGAT 
TTCCCGGGCT TCCTGGCGGA TGAGTTCATT GCACTGTCAC CACCACAGCC ACAGGATTAC 
CACTTCGGCC TGGAGGAGGG TGAGGGCATC AGTGAGCTCT TTGACTGTGA CTTTGGGGAC 
TTCACGCACT TGGACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015151824.1
CDS	102..1322
Translation	MATAGGAAGLAALLGGASPHLLIVSASEEPAGGCRPDADLLLFATPQPSRPGPAPRRPALGRPPVKRKLNLETDHQYIAESLPAARGRARIPGRGAKSPGEKSRYETSLNLTTKRFLELLSQSPDGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLITKKSKNNIQWLGSQVAAGASSRQRLLEKELRDLQAAERQLDDLIQTCTVQLRLLTEDPSNQHAAYVTCQDLRSIVDPSEQMVMVIKAPPETQLQVSDPGEAFQVSVRSTQGPIDVFLCPEDSSGVCSPVKSPFKAPAEELSPGSSQQRASPLLHSAQDVNMLLPGEQEALLPGTALPTKCPTEDVSLSPLASMDTLLEHGKDDFPGFLADEFIALSPPQPQDYHFGLEEGEGISELFDCDFGDFTHLDF

Target ORF information:

RefSeq Version	XM_015296338.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus E2F transcription factor 1 (E2F1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015296338.2

ORF Insert Sequence:

ATGGCTACGG CGGGCGGCGC GGCGGGGCTG GCGGCGCTGC TGGGCGGCGC CTCCCCGCAC 
CTCCTCATCG TCTCGGCCTC CGAGGAGCCC GCGGGCGGCT GCCGCCCCGA CGCCGACCTC 
CTGCTCTTCG CCACGCCGCA GCCCTCCCGC CCCGGCCCCG CGCCGCGCCG CCCCGCGCTG 
GGCCGCCCGC CGGTGAAGCG GAAGCTGAAC TTGGAGACGG ATCACCAGTA CATAGCCGAG 
AGCCTGCCCG CGGCCCGCGG CAGGGCCCGG ATCCCCGGCA GAGGGGCAAA GTCTCCTGGG 
GAGAAGTCCC GCTATGAAAC CTCGCTGAAC CTCACCACCA AGCGCTTCCT GGAGCTGCTC 
AGCCAATCCC CCGACGGTGT GGTGGACCTC AACTGGGCGG CCGAGGTGCT GAAGGTGCAG 
AAGAGGCGCA TCTACGACAT CACCAATGTC CTGGAGGGCA TCCAGCTCAT CACCAAGAAG 
TCCAAGAACA ACATCCAGTG GCTGGGCAGC CAGGTGGCCG CGGGGGCCTC CAGCCGACAG 
CGGCTGCTGG AGAAGGAGCT GCGGGATCTG CAGGCGGCCG AGCGGCAGCT GGACGATCTC 
ATCCAGACGT GCACGGTGCA GCTGCGGCTG CTCACTGAGG ATCCCTCCAA CCAGCACGCA 
GCCTACGTCA CCTGCCAGGA CCTCCGGAGC ATCGTGGACC CCTCTGAGCA AATGGTGATG 
GTTATCAAGG CACCCCCAGA GACCCAGCTG CAAGTCTCGG ACCCGGGAGA GGCTTTCCAG 
GTCTCCGTGA GAAGCACTCA GGGCCCCATC GACGTGTTCC TCTGTCCCGA GGACAGCTCA 
GGGGTCTGCA GCCCCGTCAA GAGCCCCTTC AAGGCACCTG CTGAGGAGCT CTCCCCCGGC 
TCATCGCAGC AGAGAGCCTC CCCGCTCCTG CATTCTGCCC AGGATGTGAA CATGCTGCTG 
CCTGGTGAGC AAGAGGCACT GCTCCCAGGC ACAGCGCTGC CGACCAAGTG CCCCACAGAG 
GACGTGAGCC TGTCGCCGCT AGCCTCCATG GACACACTCC TGGAGCATGG CAAGGACGAT 
TTCCCGGGCT TCCTGGCGGA TGAGTTCATT GCACTGTCAC CACCACAGCC ACAGGATTAC 
CACTTCGGCC TGGAGGAGGG TGAGGGCATC AGTGAGCTCT TTGACTGTGA CTTTGGGGAC 
TTCACGCACT TGGACTTCTG A