WSB1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	WSB1
Entrez Gene ID	395685
Full Name	WD repeat and SOCS box containing 1
Synonyms	SWIP-1
General protein information	Preferred Names WD repeat and SOCS box containing 1 Names WD repeat and SOCS box-containing protein 1 SOCS box-containing WD protein SWiP-1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204872.1	NP_990203.1	WD repeat and SOCS box-containing protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	WSB1	XP_001149775.1
Bos taurus (cattle)	WSB1	XP_002695684.2
Gallus gallus (chicken)	WSB1	NP_990203.1
Homo sapiens (human)	WSB1	NP_056441.6
	WSB1	XP_001105419.1
Mus musculus (house mouse)	Wsb1	NP_062627.3
Rattus norvegicus (Norway rat)	Wsb1	NP_001036026.1
Danio rerio (zebrafish)	wsb1	NP_955927.1
Xenopus tropicalis (tropical clawed frog)	wsb1	NP_989387.1
Canis lupus familiaris (dog)	WSB1	XP_853945.1

Gene Ontology
Bibliography

Related articles in PubMed

The Hedgehog-inducible ubiquitin ligase subunit WSB-1 modulates thyroid hormone activation and PTHrP secretion in the developing growth plate.
Dentice M, Bandyopadhyay A, Gereben B, Callebaut I, Christoffolete MA, Kim BW, Nissim S, Mornon JP, Zavacki AM, Ze?ld A, Capelo LP, Curcio-Morelli C, Ribeiro R, Harney JW, Tabin CJ, Bianco AC
Nature cell biology7(7)698-705(2005 Jul)

SWiP-1: novel SOCS box containing WD-protein regulated by signalling centres and by Shh during development.
Vasiliauskas D, Hancock S, Stern CD
Mechanisms of development82(1-2)79-94(1999 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the developing tibial growth plate, Hedgehog-stimulated D2 ubiquitination via ECS(WSB-1) induces parathyroid hormone-related peptide (PTHrP), thereby regulating chondrocyte differentiation.
Title: The Hedgehog-inducible ubiquitin ligase subunit WSB-1 modulates thyroid hormone activation and PTHrP secretion in the developing growth plate.

The following WSB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WSB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa23947	NM_204872.1 Latest version!	Gallus gallus WD repeat and SOCS box containing 1 (WSB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WSB1 ( NM_204872.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990203.1 Gallus gallus WD repeat and SOCS box containing 1 (WSB1), mRNA.

Price & Availability↑

CloneID	OGa23947
Clone ID Related Accession (Same CDS sequence)	NM_204872.1
Accession Version	NM_204872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	WD repeat and SOCS box-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF072879.1. Sequence Note: This sequence has been modified as follows: removed 18 bp suspected to be vector contamination from the 3' end. ##Evidence-Data-START## Transcript exon combination :: AF072879.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCAGCT TTCCCCTGAG CGTCAACGAG AAGCTCATCG CACGGTCGCG GGCTGTTGGA 
GAACTCTTGG CCCCCACTTC TCCTTTCGAC AAGAAGTGTG GACGTGAGAA CTGGACTGTT 
GCGTTTGCTC CTGATGGATC CTACTTTGCT TGGTCGCAAG GACATCGTAT AGTGAAGCTG 
GTCCCCTGGT CTCAGTGCCT TACTAACTTC TTGTTGCATG GCACAAAGAA TGTTGCAAAT 
TCTGCCAATG CAAGGCTTTC AAGACAGAAC AGTGACGGCA GTCAGAGAAA CAAGCCCTGT 
GAACATATAA TAGACTGCGG TGATATTGTC TGGAGTCTGG CTTTTGGGTC TTCAGTGCCT 
GAGAAGCAGA GTCGCTGTGT GAATATAGAA TGGCATCGTT TCAAATTTGG GCAGGACCAG 
CTGCTGCTTG CAACAGGCCT GAACAATGGG CGCATCAAGA TCTGGGATGT GTATACAGGA 
AAACTCCTCC TTAACCTGAT GGACCATACA GAAGTTGTCA GAGATTTAAC GTTTGCCCCA 
GATGGCAGCC TGATTTTGGT GTCTGCATCC AGAGACAAAA CACTGAGAGT GTGGGACCTG 
AAAGATGACG GAAATATGAT GAAAGTGTTG AGAGGACATC AGAACTGGGT ATATGGTTGT 
GCATTCTCTC CAGATTCTTC CATTCTCTGT TCTGTTGGAG CTAGTAAGGC AGTTTTTCTC 
TGGGATATGG ATAAATACTC CATGATACGA AAACTTGAAG GACATCACAA TGATGTTGTA 
GCCTGTGAGT TTTCTCCTGA TGGAGCTTTA CTGGCTACTG CATCCTACGA TACTCGAGTC 
TATGTCTGGG ATCCATACAT TGGAGTTATT CTTAGGGAGT TTGGGCATCT GTTTCCACCT 
CCTACACCAA TATTTGCTGG AGGTGCAAAC GACCGATGGG TTAGATCAGT GTCTTTTAGT 
CACGATGGAC TACATATTGC AAGCCTTGCT GATGATAAAA TGGTAAGATT CTGGAGAATT 
GATGAAGAGT ACCCTGTACA AGTTGCACCT CTGAACAACG GGCTCTGCTG TACCTTTTCT 
ACTGATGGCA GTGTTCTAGC TGCAGGGACA CAGGATGGCA GTGTGCACTT CTGGGCAACT 
CCAAGACAAG TCTCTAGTCT CCAGCATTTG TGTCGCATGG CAATTCGAAG AGTGATGCCC 
ACAAGCCAAG TCAAGAACTT GCCTACTCCA TCAAAAGTGG TGGAATTTCT TTGTTACCAG 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990203.1
CDS	80..1345
Translation	MASFPLSVNEKLIARSRAVGELLAPTSPFDKKCGRENWTVAFAPDGSYFAWSQGHRIVKLVPWSQCLTNFLLHGTKNVANSANARLSRQNSDGSQRNKPCEHIIDCGDIVWSLAFGSSVPEKQSRCVNIEWHRFKFGQDQLLLATGLNNGRIKIWDVYTGKLLLNLMDHTEVVRDLTFAPDGSLILVSASRDKTLRVWDLKDDGNMMKVLRGHQNWVYGCAFSPDSSILCSVGASKAVFLWDMDKYSMIRKLEGHHNDVVACEFSPDGALLATASYDTRVYVWDPYIGVILREFGHLFPPPTPIFAGGANDRWVRSVSFSHDGLHIASLADDKMVRFWRIDEEYPVQVAPLNNGLCCTFSTDGSVLAAGTQDGSVHFWATPRQVSSLQHLCRMAIRRVMPTSQVKNLPTPSKVVEFLCYQI

Target ORF information:

RefSeq Version	NM_204872.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus WD repeat and SOCS box containing 1 (WSB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204872.1

ORF Insert Sequence:

ATGGCCAGCT TTCCCCTGAG CGTCAACGAG AAGCTCATCG CACGGTCGCG GGCTGTTGGA 
GAACTCTTGG CCCCCACTTC TCCTTTCGAC AAGAAGTGTG GACGTGAGAA CTGGACTGTT 
GCGTTTGCTC CTGATGGATC CTACTTTGCT TGGTCGCAAG GACATCGTAT AGTGAAGCTG 
GTCCCCTGGT CTCAGTGCCT TACTAACTTC TTGTTGCATG GCACAAAGAA TGTTGCAAAT 
TCTGCCAATG CAAGGCTTTC AAGACAGAAC AGTGACGGCA GTCAGAGAAA CAAGCCCTGT 
GAACATATAA TAGACTGCGG TGATATTGTC TGGAGTCTGG CTTTTGGGTC TTCAGTGCCT 
GAGAAGCAGA GTCGCTGTGT GAATATAGAA TGGCATCGTT TCAAATTTGG GCAGGACCAG 
CTGCTGCTTG CAACAGGCCT GAACAATGGG CGCATCAAGA TCTGGGATGT GTATACAGGA 
AAACTCCTCC TTAACCTGAT GGACCATACA GAAGTTGTCA GAGATTTAAC GTTTGCCCCA 
GATGGCAGCC TGATTTTGGT GTCTGCATCC AGAGACAAAA CACTGAGAGT GTGGGACCTG 
AAAGATGACG GAAATATGAT GAAAGTGTTG AGAGGACATC AGAACTGGGT ATATGGTTGT 
GCATTCTCTC CAGATTCTTC CATTCTCTGT TCTGTTGGAG CTAGTAAGGC AGTTTTTCTC 
TGGGATATGG ATAAATACTC CATGATACGA AAACTTGAAG GACATCACAA TGATGTTGTA 
GCCTGTGAGT TTTCTCCTGA TGGAGCTTTA CTGGCTACTG CATCCTACGA TACTCGAGTC 
TATGTCTGGG ATCCATACAT TGGAGTTATT CTTAGGGAGT TTGGGCATCT GTTTCCACCT 
CCTACACCAA TATTTGCTGG AGGTGCAAAC GACCGATGGG TTAGATCAGT GTCTTTTAGT 
CACGATGGAC TACATATTGC AAGCCTTGCT GATGATAAAA TGGTAAGATT CTGGAGAATT 
GATGAAGAGT ACCCTGTACA AGTTGCACCT CTGAACAACG GGCTCTGCTG TACCTTTTCT 
ACTGATGGCA GTGTTCTAGC TGCAGGGACA CAGGATGGCA GTGTGCACTT CTGGGCAACT 
CCAAGACAAG TCTCTAGTCT CCAGCATTTG TGTCGCATGG CAATTCGAAG AGTGATGCCC 
ACAAGCCAAG TCAAGAACTT GCCTACTCCA TCAAAAGTGG TGGAATTTCT TTGTTACCAG 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Hedgehog-inducible ubiquitin ligase subunit WSB-1 modulates thyroid hormone activation and PTHrP secretion in the developing growth plate.
Nature cell biology7(7)698-705(2005 Jul)
Dentice M,Bandyopadhyay A,Gereben B,Callebaut I,Christoffolete MA,Kim BW,Nissim S,Mornon JP,Zavacki AM,Ze?ld A,Capelo LP,Curcio-Morelli C,Ribeiro R,Harney JW,Tabin CJ,Bianco AC

SWiP-1: novel SOCS box containing WD-protein regulated by signalling centres and by Shh during development.
Mechanisms of development82(1-2)79-94(1999 Apr)
Vasiliauskas D,Hancock S,Stern CD