CASP3 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CASP3
Entrez Gene ID	395476
Full Name	caspase 3
Synonyms	caspase-3
General protein information	Preferred Names caspase 3 Names caspase-3 caspase 3, apoptosis-related cysteine peptidase caspase 3, apoptosis-related cysteine protease
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015276123.2	XP_015131609.1	caspase-3 isoform X1
XM_015276122.1	XP_015131608.1	caspase-3 isoform X1
XM_015276123.1	XP_015131609.1	caspase-3 isoform X1
NM_204725.1	NP_990056.1	caspase-3
XM_015276122.2	XP_015131608.1	caspase-3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CASP3	NP_004337.2
Canis lupus familiaris (dog)	CASP3	NP_001003042.1
Rattus norvegicus (Norway rat)	Casp3	NP_037054.1
Gallus gallus (chicken)	CASP3	NP_990056.1
	CASP3	XP_001083044.1
Anopheles gambiae (African malaria mosquito)	CASPS8	XP_318945.3
Xenopus tropicalis (tropical clawed frog)	casp3	NP_001120900.1
Pan troglodytes (chimpanzee)	CASP3	NP_001012435.1
Bos taurus (cattle)	CASP3	NP_001071308.1
Mus musculus (house mouse)	Casp3	NP_001271338.1

Gene Ontology
Bibliography

Related articles in PubMed

Avian reovirus-triggered apoptosis enhances both virus spread and the processing of the viral nonstructural muNS protein.
Rodr?guez-Grille J, Busch LK, Mart?nez-Costas J, Benavente J
Virology462-46349-59(2014 Aug)

The association between splenocyte apoptosis and alterations of Bax, Bcl-2 and caspase-3 mRNA expression, and oxidative stress induced by dietary nickel chloride in broilers.
Huang J, Cui H, Peng X, Fang J, Zuo Z, Deng J, Wu B
International journal of environmental research and public health10(12)7310-26(2013 Dec)

Dietary high fluorine induces apoptosis and alters Bcl-2, Bax, and caspase-3 protein expression in the cecal tonsil lymphocytes of broilers.
Liu J, Cui H, Peng X, Fang J, Zuo Z, Wang H, Wu B, Deng Y, Wang K
Biological trace element research152(1)25-30(2013 Apr)

Developmental truncations of connexin 50 by caspases adaptively regulate gap junctions/hemichannels and protect lens cells against ultraviolet radiation.
Wang K, Gu S, Yin X, Weintraub ST, Hua Z, Jiang JX
The Journal of biological chemistry287(19)15786-97(2012 May)

Insulin-like growth factor receptor-1 and nuclear factor ?B are crucial survival signals that regulate caspase-3-mediated lens epithelial cell differentiation initiation.
Basu S, Rajakaruna S, Menko AS
The Journal of biological chemistry287(11)8384-97(2012 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Finally, the inhibition of chicken caspase activity in avian reovirus-infected cells does not diminish virus gene expression and replication, but drastically reduces muNS processing and the release and dissemination of progeny viral particles.
Title: Avian reovirus-triggered apoptosis enhances both virus spread and the processing of the viral nonstructural muNS protein.

mRNA expression was higher in Bax and Caspase-3, and lower in Bcl-2 of the 300, 600 and 900 mg/kg groups. SOD, CAT and GSH-Px activities, ability to inhibit hydroxyl radical, and GSH contents were decreased, and MDA contents were increased in all groups.
Title: The association between splenocyte apoptosis and alterations of Bax, Bcl-2 and caspase-3 mRNA expression, and oxidative stress induced by dietary nickel chloride in broilers.

Data suggest that lymphocyte apoptosis in the cecal tonsil of broilers was mediated by direct effects of fluoride on the expression of Bcl-2, Bax, and caspase-3: [bax]
Title: Dietary high fluorine induces apoptosis and alters Bcl-2, Bax, and caspase-3 protein expression in the cecal tonsil lymphocytes of broilers.

Results suggest that both caspase-1 and -3 are responsible for the cleavage at the C terminus of connexin 50 (Cx50) during lens development.
Title: Developmental truncations of connexin 50 by caspases adaptively regulate gap junctions/hemichannels and protect lens cells against ultraviolet radiation.

the IGF-1R/NFkappaB cell survival signal is a crucial regulator of the level of caspase-3 activation for its non-apoptotic function in signaling cell differentiation.
Title: Insulin-like growth factor receptor-1 and nuclear factor ?B are crucial survival signals that regulate caspase-3-mediated lens epithelial cell differentiation initiation.

The following CASP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CASP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa26413	NM_204725.1 Latest version!	Gallus gallus caspase 3 (CASP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OGa35007	XM_015276122.2 Latest version!	Gallus gallus caspase 3 (CASP3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OGa35007	XM_015276123.2 Latest version!	Gallus gallus caspase 3 (CASP3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
OGa35007	XM_015276123.1	Gallus gallus caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OGa35007	XM_015276122.1	Gallus gallus caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CASP3 ( NM_204725.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990056.1 Gallus gallus caspase 3 (CASP3), mRNA.

Price & Availability↑

CloneID	OGa26413
Clone ID Related Accession (Same CDS sequence)	NM_204725.1
Accession Version	NM_204725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	caspase-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF083029.1. ##Evidence-Data-START## Transcript exon combination :: AF083029.1 [ECO:0000332] ##Evidence-Data-END##

ATGATGACAG ACATAAAAGA TGGACCACGC TCAGGGGAAG ATGTATCAGA TGCAAGATCT 
TTCCCTGGTT CCAAAGGAAT GAACTTACCT GCTAGCAAGT CTGTGGACTC TGGAATTCTG 
CCTGATGACA GTTACAGAAT GGATTATCCA GAGATAGGAG TATGTGTTAT AATAAACAAT 
AAGAACTTCC ACCGAGATAC CGGACTGTCA TCTCGTTCAG GCACGGATGC AGATGCTGCA 
AGTGTCAGAG AAGTTTTTAT GAAGCTGGGA TATAAAGTCA AGCTTAACAA TGATCTGTCA 
AGCAGAGATA TTTTTAAGCT ATTGAAAAAT GTTTCTGAAG AAGATCACAG CAAGCGAAGC 
AGTTTTGTTT GTGTGTTGCT AAGCCATGGC GATGAAGGAC TCTTCTATGG TACAGATGGC 
CCTCTTGAAC TGAAAGTACT AACCAGCCTT TTCAGAGGTG ACAAGTGCAG AAGTCTAGCA 
GGGAAACCCA AACTCTTTTT CATTCAGGCC TGTAGAGGAA CAGAATTAGA TTCTGGTATT 
GAAGCAGACA GTGGACCAGA TGAAACAGTG TGTCAAAAAA TACCTGTAGA AGCAGACTTC 
CTGTATGCAT ATTCTACTGC TCCAGGCTAC TACTCCTGGA GGAACGCAGC TGAAGGCTCC 
TGGTTTATTC AGTCTCTGTG TAGGATGCTG AAGGAACACG CCAGGAAACT TGAACTCATG 
CAGATTTTAA CTCGTGTAAA TCGCAGAGTG GCAGAATATG AATCCTGCTC CACTCGACAG 
GATTTCAATG CAAAGAAACA GATTCCATGC ATTGTGTCTA TGCTTACCAA AGAATTCTAC 
TTTCCTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990056.1
CDS	171..1022
Translation	MMTDIKDGPRSGEDVSDARSFPGSKGMNLPASKSVDSGILPDDSYRMDYPEIGVCVIINNKNFHRDTGLSSRSGTDADAASVREVFMKLGYKVKLNNDLSSRDIFKLLKNVSEEDHSKRSSFVCVLLSHGDEGLFYGTDGPLELKVLTSLFRGDKCRSLAGKPKLFFIQACRGTELDSGIEADSGPDETVCQKIPVEADFLYAYSTAPGYYSWRNAAEGSWFIQSLCRMLKEHARKLELMQILTRVNRRVAEYESCSTRQDFNAKKQIPCIVSMLTKEFYFPC

Target ORF information:

RefSeq Version	NM_204725.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus caspase 3 (CASP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204725.1

ORF Insert Sequence:

ATGATGACAG ACATAAAAGA TGGACCACGC TCAGGGGAAG ATGTATCAGA TGCAAGATCT 
TTCCCTGGTT CCAAAGGAAT GAACTTACCT GCTAGCAAGT CTGTGGACTC TGGAATTCTG 
CCTGATGACA GTTACAGAAT GGATTATCCA GAGATAGGAG TATGTGTTAT AATAAACAAT 
AAGAACTTCC ACCGAGATAC CGGACTGTCA TCTCGTTCAG GCACGGATGC AGATGCTGCA 
AGTGTCAGAG AAGTTTTTAT GAAGCTGGGA TATAAAGTCA AGCTTAACAA TGATCTGTCA 
AGCAGAGATA TTTTTAAGCT ATTGAAAAAT GTTTCTGAAG AAGATCACAG CAAGCGAAGC 
AGTTTTGTTT GTGTGTTGCT AAGCCATGGC GATGAAGGAC TCTTCTATGG TACAGATGGC 
CCTCTTGAAC TGAAAGTACT AACCAGCCTT TTCAGAGGTG ACAAGTGCAG AAGTCTAGCA 
GGGAAACCCA AACTCTTTTT CATTCAGGCC TGTAGAGGAA CAGAATTAGA TTCTGGTATT 
GAAGCAGACA GTGGACCAGA TGAAACAGTG TGTCAAAAAA TACCTGTAGA AGCAGACTTC 
CTGTATGCAT ATTCTACTGC TCCAGGCTAC TACTCCTGGA GGAACGCAGC TGAAGGCTCC 
TGGTTTATTC AGTCTCTGTG TAGGATGCTG AAGGAACACG CCAGGAAACT TGAACTCATG 
CAGATTTTAA CTCGTGTAAA TCGCAGAGTG GCAGAATATG AATCCTGCTC CACTCGACAG 
GATTTCAATG CAAAGAAACA GATTCCATGC ATTGTGTCTA TGCTTACCAA AGAATTCTAC 
TTTCCTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASP3 ( XM_015276123.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131609.1 Gallus gallus caspase 3 (CASP3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa35007
Clone ID Related Accession (Same CDS sequence)	XM_015276123.2 , XM_015276122.1 , XM_015276123.1 , XM_015276122.2
Accession Version	XM_015276123.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	caspase-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276123.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAG ACATAAAAGA TGGACCACGC TCAGGGGAAG ATGTATCAGA TGCAAGATCT 
TTCCCTGGTT CCAAAGGAAT GAACTTACCT GCTAGCAAGT CTGTGGACTC TGGAATTCTG 
CCTGATGACA GTTACAGAAT GGATTATCCA GAGATAGGAG TATGTGTTAT AATAAACAAT 
AAGAACTTCC ACCGAGATAC CGGACTGTCA TCTCGTTCAG GCACGGATGC AGATGCTGCA 
AGTGTCAGAG AAGTTTTTAT GAAGCTGGGA TATAAAGTCA AGCTTAACAA TGATCTGTCA 
AGCAGAGATA TTTTTAAGCT ATTGAAAAAT GTTTCTGAAG AAGATCACAG CAAGCGAAGC 
AGTTTTGTTT GTGTGTTGCT AAGCCATGGC GATGAAGGAC TCTTCTATGG TACAGATGGC 
CCTCTTGAAC TGAAAGTACT AACCAGCCTT TTCAGAGGTG ACAAGTGCAG AAGTCTAGCG 
GGGAAACCCA AACTCTTTTT CATTCAGGCC TGTAGAGGAA CAGAATTAGA TTCTGGTATT 
GAAGCAGACA GTGGACCAGA TGAAACAGTG TGTCAAAAAA TACCTGTAGA AGCAGACTTC 
CTGTATGCAT ATTCTACTGC TCCAGGCTAC TACTCCTGGA GGAACGCAGC TGAAGGCTCC 
TGGTTTATTC AGTCTCTGTG TAGGATGCTG AAGGAACACG CCAGGAAACT TGAACTCATG 
CAGATTTTAA CTCGTGTAAA TCGCAGAGTG GCAGAATATG AATCCTGCTC CACTCGACAG 
GATTTCAATG CAAAGAAACA GATTCCATGC ATTGTGTCTA TGCTTACCAA AGAATTCTAC 
TTTCCTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131609.1
CDS	336..1187
Translation	MMTDIKDGPRSGEDVSDARSFPGSKGMNLPASKSVDSGILPDDSYRMDYPEIGVCVIINNKNFHRDTGLSSRSGTDADAASVREVFMKLGYKVKLNNDLSSRDIFKLLKNVSEEDHSKRSSFVCVLLSHGDEGLFYGTDGPLELKVLTSLFRGDKCRSLAGKPKLFFIQACRGTELDSGIEADSGPDETVCQKIPVEADFLYAYSTAPGYYSWRNAAEGSWFIQSLCRMLKEHARKLELMQILTRVNRRVAEYESCSTRQDFNAKKQIPCIVSMLTKEFYFPC

Target ORF information:

RefSeq Version	XM_015276123.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus caspase 3 (CASP3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276123.2

ORF Insert Sequence:

ATGATGACAG ACATAAAAGA TGGACCACGC TCAGGGGAAG ATGTATCAGA TGCAAGATCT 
TTCCCTGGTT CCAAAGGAAT GAACTTACCT GCTAGCAAGT CTGTGGACTC TGGAATTCTG 
CCTGATGACA GTTACAGAAT GGATTATCCA GAGATAGGAG TATGTGTTAT AATAAACAAT 
AAGAACTTCC ACCGAGATAC CGGACTGTCA TCTCGTTCAG GCACGGATGC AGATGCTGCA 
AGTGTCAGAG AAGTTTTTAT GAAGCTGGGA TATAAAGTCA AGCTTAACAA TGATCTGTCA 
AGCAGAGATA TTTTTAAGCT ATTGAAAAAT GTTTCTGAAG AAGATCACAG CAAGCGAAGC 
AGTTTTGTTT GTGTGTTGCT AAGCCATGGC GATGAAGGAC TCTTCTATGG TACAGATGGC 
CCTCTTGAAC TGAAAGTACT AACCAGCCTT TTCAGAGGTG ACAAGTGCAG AAGTCTAGCG 
GGGAAACCCA AACTCTTTTT CATTCAGGCC TGTAGAGGAA CAGAATTAGA TTCTGGTATT 
GAAGCAGACA GTGGACCAGA TGAAACAGTG TGTCAAAAAA TACCTGTAGA AGCAGACTTC 
CTGTATGCAT ATTCTACTGC TCCAGGCTAC TACTCCTGGA GGAACGCAGC TGAAGGCTCC 
TGGTTTATTC AGTCTCTGTG TAGGATGCTG AAGGAACACG CCAGGAAACT TGAACTCATG 
CAGATTTTAA CTCGTGTAAA TCGCAGAGTG GCAGAATATG AATCCTGCTC CACTCGACAG 
GATTTCAATG CAAAGAAACA GATTCCATGC ATTGTGTCTA TGCTTACCAA AGAATTCTAC 
TTTCCTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASP3 ( XM_015276122.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131608.1 Gallus gallus caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa35007
Clone ID Related Accession (Same CDS sequence)	XM_015276123.2 , XM_015276122.1 , XM_015276123.1 , XM_015276122.2
Accession Version	XM_015276122.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	caspase-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAG ACATAAAAGA TGGACCACGC TCAGGGGAAG ATGTATCAGA TGCAAGATCT 
TTCCCTGGTT CCAAAGGAAT GAACTTACCT GCTAGCAAGT CTGTGGACTC TGGAATTCTG 
CCTGATGACA GTTACAGAAT GGATTATCCA GAGATAGGAG TATGTGTTAT AATAAACAAT 
AAGAACTTCC ACCGAGATAC CGGACTGTCA TCTCGTTCAG GCACGGATGC AGATGCTGCA 
AGTGTCAGAG AAGTTTTTAT GAAGCTGGGA TATAAAGTCA AGCTTAACAA TGATCTGTCA 
AGCAGAGATA TTTTTAAGCT ATTGAAAAAT GTTTCTGAAG AAGATCACAG CAAGCGAAGC 
AGTTTTGTTT GTGTGTTGCT AAGCCATGGC GATGAAGGAC TCTTCTATGG TACAGATGGC 
CCTCTTGAAC TGAAAGTACT AACCAGCCTT TTCAGAGGTG ACAAGTGCAG AAGTCTAGCG 
GGGAAACCCA AACTCTTTTT CATTCAGGCC TGTAGAGGAA CAGAATTAGA TTCTGGTATT 
GAAGCAGACA GTGGACCAGA TGAAACAGTG TGTCAAAAAA TACCTGTAGA AGCAGACTTC 
CTGTATGCAT ATTCTACTGC TCCAGGCTAC TACTCCTGGA GGAACGCAGC TGAAGGCTCC 
TGGTTTATTC AGTCTCTGTG TAGGATGCTG AAGGAACACG CCAGGAAACT TGAACTCATG 
CAGATTTTAA CTCGTGTAAA TCGCAGAGTG GCAGAATATG AATCCTGCTC CACTCGACAG 
GATTTCAATG CAAAGAAACA GATTCCATGC ATTGTGTCTA TGCTTACCAA AGAATTCTAC 
TTTCCTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131608.1
CDS	260..1111
Translation	MMTDIKDGPRSGEDVSDARSFPGSKGMNLPASKSVDSGILPDDSYRMDYPEIGVCVIINNKNFHRDTGLSSRSGTDADAASVREVFMKLGYKVKLNNDLSSRDIFKLLKNVSEEDHSKRSSFVCVLLSHGDEGLFYGTDGPLELKVLTSLFRGDKCRSLAGKPKLFFIQACRGTELDSGIEADSGPDETVCQKIPVEADFLYAYSTAPGYYSWRNAAEGSWFIQSLCRMLKEHARKLELMQILTRVNRRVAEYESCSTRQDFNAKKQIPCIVSMLTKEFYFPC

Target ORF information:

RefSeq Version	XM_015276122.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276122.1

ORF Insert Sequence:

ATGATGACAG ACATAAAAGA TGGACCACGC TCAGGGGAAG ATGTATCAGA TGCAAGATCT 
TTCCCTGGTT CCAAAGGAAT GAACTTACCT GCTAGCAAGT CTGTGGACTC TGGAATTCTG 
CCTGATGACA GTTACAGAAT GGATTATCCA GAGATAGGAG TATGTGTTAT AATAAACAAT 
AAGAACTTCC ACCGAGATAC CGGACTGTCA TCTCGTTCAG GCACGGATGC AGATGCTGCA 
AGTGTCAGAG AAGTTTTTAT GAAGCTGGGA TATAAAGTCA AGCTTAACAA TGATCTGTCA 
AGCAGAGATA TTTTTAAGCT ATTGAAAAAT GTTTCTGAAG AAGATCACAG CAAGCGAAGC 
AGTTTTGTTT GTGTGTTGCT AAGCCATGGC GATGAAGGAC TCTTCTATGG TACAGATGGC 
CCTCTTGAAC TGAAAGTACT AACCAGCCTT TTCAGAGGTG ACAAGTGCAG AAGTCTAGCG 
GGGAAACCCA AACTCTTTTT CATTCAGGCC TGTAGAGGAA CAGAATTAGA TTCTGGTATT 
GAAGCAGACA GTGGACCAGA TGAAACAGTG TGTCAAAAAA TACCTGTAGA AGCAGACTTC 
CTGTATGCAT ATTCTACTGC TCCAGGCTAC TACTCCTGGA GGAACGCAGC TGAAGGCTCC 
TGGTTTATTC AGTCTCTGTG TAGGATGCTG AAGGAACACG CCAGGAAACT TGAACTCATG 
CAGATTTTAA CTCGTGTAAA TCGCAGAGTG GCAGAATATG AATCCTGCTC CACTCGACAG 
GATTTCAATG CAAAGAAACA GATTCCATGC ATTGTGTCTA TGCTTACCAA AGAATTCTAC 
TTTCCTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASP3 ( XM_015276123.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131609.1 Gallus gallus caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa35007
Clone ID Related Accession (Same CDS sequence)	XM_015276123.2 , XM_015276122.1 , XM_015276123.1 , XM_015276122.2
Accession Version	XM_015276123.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	caspase-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471685.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAG ACATAAAAGA TGGACCACGC TCAGGGGAAG ATGTATCAGA TGCAAGATCT 
TTCCCTGGTT CCAAAGGAAT GAACTTACCT GCTAGCAAGT CTGTGGACTC TGGAATTCTG 
CCTGATGACA GTTACAGAAT GGATTATCCA GAGATAGGAG TATGTGTTAT AATAAACAAT 
AAGAACTTCC ACCGAGATAC CGGACTGTCA TCTCGTTCAG GCACGGATGC AGATGCTGCA 
AGTGTCAGAG AAGTTTTTAT GAAGCTGGGA TATAAAGTCA AGCTTAACAA TGATCTGTCA 
AGCAGAGATA TTTTTAAGCT ATTGAAAAAT GTTTCTGAAG AAGATCACAG CAAGCGAAGC 
AGTTTTGTTT GTGTGTTGCT AAGCCATGGC GATGAAGGAC TCTTCTATGG TACAGATGGC 
CCTCTTGAAC TGAAAGTACT AACCAGCCTT TTCAGAGGTG ACAAGTGCAG AAGTCTAGCG 
GGGAAACCCA AACTCTTTTT CATTCAGGCC TGTAGAGGAA CAGAATTAGA TTCTGGTATT 
GAAGCAGACA GTGGACCAGA TGAAACAGTG TGTCAAAAAA TACCTGTAGA AGCAGACTTC 
CTGTATGCAT ATTCTACTGC TCCAGGCTAC TACTCCTGGA GGAACGCAGC TGAAGGCTCC 
TGGTTTATTC AGTCTCTGTG TAGGATGCTG AAGGAACACG CCAGGAAACT TGAACTCATG 
CAGATTTTAA CTCGTGTAAA TCGCAGAGTG GCAGAATATG AATCCTGCTC CACTCGACAG 
GATTTCAATG CAAAGAAACA GATTCCATGC ATTGTGTCTA TGCTTACCAA AGAATTCTAC 
TTTCCTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131609.1
CDS	1427..2278
Translation	MMTDIKDGPRSGEDVSDARSFPGSKGMNLPASKSVDSGILPDDSYRMDYPEIGVCVIINNKNFHRDTGLSSRSGTDADAASVREVFMKLGYKVKLNNDLSSRDIFKLLKNVSEEDHSKRSSFVCVLLSHGDEGLFYGTDGPLELKVLTSLFRGDKCRSLAGKPKLFFIQACRGTELDSGIEADSGPDETVCQKIPVEADFLYAYSTAPGYYSWRNAAEGSWFIQSLCRMLKEHARKLELMQILTRVNRRVAEYESCSTRQDFNAKKQIPCIVSMLTKEFYFPC

Target ORF information:

RefSeq Version	XM_015276123.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276123.1

ORF Insert Sequence:

ATGATGACAG ACATAAAAGA TGGACCACGC TCAGGGGAAG ATGTATCAGA TGCAAGATCT 
TTCCCTGGTT CCAAAGGAAT GAACTTACCT GCTAGCAAGT CTGTGGACTC TGGAATTCTG 
CCTGATGACA GTTACAGAAT GGATTATCCA GAGATAGGAG TATGTGTTAT AATAAACAAT 
AAGAACTTCC ACCGAGATAC CGGACTGTCA TCTCGTTCAG GCACGGATGC AGATGCTGCA 
AGTGTCAGAG AAGTTTTTAT GAAGCTGGGA TATAAAGTCA AGCTTAACAA TGATCTGTCA 
AGCAGAGATA TTTTTAAGCT ATTGAAAAAT GTTTCTGAAG AAGATCACAG CAAGCGAAGC 
AGTTTTGTTT GTGTGTTGCT AAGCCATGGC GATGAAGGAC TCTTCTATGG TACAGATGGC 
CCTCTTGAAC TGAAAGTACT AACCAGCCTT TTCAGAGGTG ACAAGTGCAG AAGTCTAGCG 
GGGAAACCCA AACTCTTTTT CATTCAGGCC TGTAGAGGAA CAGAATTAGA TTCTGGTATT 
GAAGCAGACA GTGGACCAGA TGAAACAGTG TGTCAAAAAA TACCTGTAGA AGCAGACTTC 
CTGTATGCAT ATTCTACTGC TCCAGGCTAC TACTCCTGGA GGAACGCAGC TGAAGGCTCC 
TGGTTTATTC AGTCTCTGTG TAGGATGCTG AAGGAACACG CCAGGAAACT TGAACTCATG 
CAGATTTTAA CTCGTGTAAA TCGCAGAGTG GCAGAATATG AATCCTGCTC CACTCGACAG 
GATTTCAATG CAAAGAAACA GATTCCATGC ATTGTGTCTA TGCTTACCAA AGAATTCTAC 
TTTCCTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASP3 ( XM_015276122.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131608.1 Gallus gallus caspase 3 (CASP3), transcript variant X2, mRNA.

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CloneID	OGa35007
Clone ID Related Accession (Same CDS sequence)	XM_015276123.2 , XM_015276122.1 , XM_015276123.1 , XM_015276122.2
Accession Version	XM_015276122.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	caspase-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276122.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAG ACATAAAAGA TGGACCACGC TCAGGGGAAG ATGTATCAGA TGCAAGATCT 
TTCCCTGGTT CCAAAGGAAT GAACTTACCT GCTAGCAAGT CTGTGGACTC TGGAATTCTG 
CCTGATGACA GTTACAGAAT GGATTATCCA GAGATAGGAG TATGTGTTAT AATAAACAAT 
AAGAACTTCC ACCGAGATAC CGGACTGTCA TCTCGTTCAG GCACGGATGC AGATGCTGCA 
AGTGTCAGAG AAGTTTTTAT GAAGCTGGGA TATAAAGTCA AGCTTAACAA TGATCTGTCA 
AGCAGAGATA TTTTTAAGCT ATTGAAAAAT GTTTCTGAAG AAGATCACAG CAAGCGAAGC 
AGTTTTGTTT GTGTGTTGCT AAGCCATGGC GATGAAGGAC TCTTCTATGG TACAGATGGC 
CCTCTTGAAC TGAAAGTACT AACCAGCCTT TTCAGAGGTG ACAAGTGCAG AAGTCTAGCG 
GGGAAACCCA AACTCTTTTT CATTCAGGCC TGTAGAGGAA CAGAATTAGA TTCTGGTATT 
GAAGCAGACA GTGGACCAGA TGAAACAGTG TGTCAAAAAA TACCTGTAGA AGCAGACTTC 
CTGTATGCAT ATTCTACTGC TCCAGGCTAC TACTCCTGGA GGAACGCAGC TGAAGGCTCC 
TGGTTTATTC AGTCTCTGTG TAGGATGCTG AAGGAACACG CCAGGAAACT TGAACTCATG 
CAGATTTTAA CTCGTGTAAA TCGCAGAGTG GCAGAATATG AATCCTGCTC CACTCGACAG 
GATTTCAATG CAAAGAAACA GATTCCATGC ATTGTGTCTA TGCTTACCAA AGAATTCTAC 
TTTCCTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131608.1
CDS	247..1098
Translation	MMTDIKDGPRSGEDVSDARSFPGSKGMNLPASKSVDSGILPDDSYRMDYPEIGVCVIINNKNFHRDTGLSSRSGTDADAASVREVFMKLGYKVKLNNDLSSRDIFKLLKNVSEEDHSKRSSFVCVLLSHGDEGLFYGTDGPLELKVLTSLFRGDKCRSLAGKPKLFFIQACRGTELDSGIEADSGPDETVCQKIPVEADFLYAYSTAPGYYSWRNAAEGSWFIQSLCRMLKEHARKLELMQILTRVNRRVAEYESCSTRQDFNAKKQIPCIVSMLTKEFYFPC

Target ORF information:

RefSeq Version	XM_015276122.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus caspase 3 (CASP3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276122.2

ORF Insert Sequence:

ATGATGACAG ACATAAAAGA TGGACCACGC TCAGGGGAAG ATGTATCAGA TGCAAGATCT 
TTCCCTGGTT CCAAAGGAAT GAACTTACCT GCTAGCAAGT CTGTGGACTC TGGAATTCTG 
CCTGATGACA GTTACAGAAT GGATTATCCA GAGATAGGAG TATGTGTTAT AATAAACAAT 
AAGAACTTCC ACCGAGATAC CGGACTGTCA TCTCGTTCAG GCACGGATGC AGATGCTGCA 
AGTGTCAGAG AAGTTTTTAT GAAGCTGGGA TATAAAGTCA AGCTTAACAA TGATCTGTCA 
AGCAGAGATA TTTTTAAGCT ATTGAAAAAT GTTTCTGAAG AAGATCACAG CAAGCGAAGC 
AGTTTTGTTT GTGTGTTGCT AAGCCATGGC GATGAAGGAC TCTTCTATGG TACAGATGGC 
CCTCTTGAAC TGAAAGTACT AACCAGCCTT TTCAGAGGTG ACAAGTGCAG AAGTCTAGCG 
GGGAAACCCA AACTCTTTTT CATTCAGGCC TGTAGAGGAA CAGAATTAGA TTCTGGTATT 
GAAGCAGACA GTGGACCAGA TGAAACAGTG TGTCAAAAAA TACCTGTAGA AGCAGACTTC 
CTGTATGCAT ATTCTACTGC TCCAGGCTAC TACTCCTGGA GGAACGCAGC TGAAGGCTCC 
TGGTTTATTC AGTCTCTGTG TAGGATGCTG AAGGAACACG CCAGGAAACT TGAACTCATG 
CAGATTTTAA CTCGTGTAAA TCGCAGAGTG GCAGAATATG AATCCTGCTC CACTCGACAG 
GATTTCAATG CAAAGAAACA GATTCCATGC ATTGTGTCTA TGCTTACCAA AGAATTCTAC 
TTTCCTTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Avian reovirus-triggered apoptosis enhances both virus spread and the processing of the viral nonstructural muNS protein.
Virology462-46349-59(2014 Aug)
Rodr?guez-Grille J,Busch LK,Mart?nez-Costas J,Benavente J

The association between splenocyte apoptosis and alterations of Bax, Bcl-2 and caspase-3 mRNA expression, and oxidative stress induced by dietary nickel chloride in broilers.
International journal of environmental research and public health10(12)7310-26(2013 Dec)
Huang J,Cui H,Peng X,Fang J,Zuo Z,Deng J,Wu B

Dietary high fluorine induces apoptosis and alters Bcl-2, Bax, and caspase-3 protein expression in the cecal tonsil lymphocytes of broilers.
Biological trace element research152(1)25-30(2013 Apr)
Liu J,Cui H,Peng X,Fang J,Zuo Z,Wang H,Wu B,Deng Y,Wang K

Developmental truncations of connexin 50 by caspases adaptively regulate gap junctions/hemichannels and protect lens cells against ultraviolet radiation.
The Journal of biological chemistry287(19)15786-97(2012 May)
Wang K,Gu S,Yin X,Weintraub ST,Hua Z,Jiang JX

Insulin-like growth factor receptor-1 and nuclear factor ?B are crucial survival signals that regulate caspase-3-mediated lens epithelial cell differentiation initiation.
The Journal of biological chemistry287(11)8384-97(2012 Mar)
Basu S,Rajakaruna S,Menko AS