ttl cDNA ORF clone, Fundulus heteroclitus(mummichog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ttl
Entrez Gene ID	105921092
Full Name	tubulin tyrosine ligase
Gene Type	protein-coding
Organism	Fundulus heteroclitus(mummichog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012856777.1	XP_012712231.1	tubulin--tyrosine ligase
XM_012856777.2	XP_012712231.1	tubulin--tyrosine ligase

Pathways from BioSystems
Gene Ontology

The following ttl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ttl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFu105483	XM_012856777.2 Latest version!	Fundulus heteroclitus tubulin tyrosine ligase (ttl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
OFu105483	XM_012856777.1	Fundulus heteroclitus tubulin tyrosine ligase (ttl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ttl ( XM_012856777.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712231.1 Fundulus heteroclitus tubulin tyrosine ligase (ttl), mRNA.

Price & Availability↑

CloneID	OFu105483
Clone ID Related Accession (Same CDS sequence)	XM_012856777.1 , XM_012856777.2
Accession Version	XM_012856777.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	tubulin--tyrosine ligase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225088.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCTC CGGTTTACAC GTTCGTTAGC CGCGACGACA ACAGCACCAT TTATGCTGAA 
GTGTCTAAAA TGCTGCTGGC CACCGGCCAC TGGAAGCGGC TGAAAAGAGA CAATCCCCGG 
TTCAACCTGA TGCTCGGGGA GCGGAACCGG CTGCCATTCG GACGCCTTGG CCATGAACCA 
GGACTGGTGC AGCTGGTGAA CTACTACAGA GGAGCAGACA AGCTTTGCAG AAAGGCATCG 
CTGGTCAAGC TAATCAAGAC CAGCCCGGAG CTGTCCGACT CCAGCAACTG GTTTCCAGAG 
TCCTACATCA TCTACCCCAC GAACCTCAAC ACCCCTGTAG CTCCGGCTTC CAACGGCATC 
AGTCACCTGA AGAGCAATCC CAAAACGGAT GAGCGGGAGG TTTTCTTGGC CTCCTACCAC 
GCCAAGAAGG AAAATGGAGA GGGAAGCGTG TGGATCGCCA AGTCTTCTGC TGGTGCTAAA 
GGAGCTGGTA TTCTGATCTC ACACGATGCA AATCAGCTGC TGGACTACAT TGACAATCAG 
GGACAGGTTC ATGTCATCCA GAAGTACCTG GAGAAACCCC TGCTGCTGGA ACCCGGTCAT 
CGCAAGTTTG ACATCAGGAG CTGGGTGCTG GTGGACCATC AGTATAACAT CTATTTGTAC 
CGGGAGGGCG TGCTGCGGAC CTCGTCGGAG CCCTACAACA GCGCGGACCT CCAGGACATG 
ACCAGCCACC TGACCAACCA CTGCATCCAG AAGGAGCACT CCCAGAACTA CGGCCGCTAC 
GAGGAGGGCA ACGAGATGTT CTTTGACGAG TTCAGGCTGT ACCTGCTGAA CACCCACAAC 
GTCACCATGG AGACAACCAT ATTACCTCAG ATCAAACACG TCATAAAGAG CTGTCTGTCC 
TGCATCGAGG CGGCGATCAG CACCAAGCAC CTCTCCTACC AGAGCTTCCA GCTCTTTGGG 
TTTGACTTCA TGGTCGACGA GAACTTCAAA GTGTGGCTCA TCGAGATCAA CGGCGCTCCA 
GCCTGCGCCC AGAAGCTCTA TCCAGAGTTG TGTCAAGGCA TCATCGACGT GGCCATCTCC 
AGTGTCTTCA CTCTCAACAG TGACTCCTCG TCTGCGTCTT CCTCGCCTTA CTCCTCCTCC 
CCTTCTTCCC TCTTCACCAC CAACTCCTGC TCCTCCCCGA AGCTGAGGGG GCCCCTGCAC 
GTGGGTCCTT TCACTCGCCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712231.1
CDS	659..1882
Translation	MSSPVYTFVSRDDNSTIYAEVSKMLLATGHWKRLKRDNPRFNLMLGERNRLPFGRLGHEPGLVQLVNYYRGADKLCRKASLVKLIKTSPELSDSSNWFPESYIIYPTNLNTPVAPASNGISHLKSNPKTDEREVFLASYHAKKENGEGSVWIAKSSAGAKGAGILISHDANQLLDYIDNQGQVHVIQKYLEKPLLLEPGHRKFDIRSWVLVDHQYNIYLYREGVLRTSSEPYNSADLQDMTSHLTNHCIQKEHSQNYGRYEEGNEMFFDEFRLYLLNTHNVTMETTILPQIKHVIKSCLSCIEAAISTKHLSYQSFQLFGFDFMVDENFKVWLIEINGAPACAQKLYPELCQGIIDVAISSVFTLNSDSSSASSSPYSSSPSSLFTTNSCSSPKLRGPLHVGPFTRL

Target ORF information:

RefSeq Version	XM_012856777.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus tubulin tyrosine ligase (ttl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012856777.1

ORF Insert Sequence:

ATGAGCTCTC CGGTTTACAC GTTCGTTAGC CGCGACGACA ACAGCACCAT TTATGCTGAA 
GTGTCTAAAA TGCTGCTGGC CACCGGCCAC TGGAAGCGGC TGAAAAGAGA CAATCCCCGG 
TTCAACCTGA TGCTCGGGGA GCGGAACCGG CTGCCATTCG GACGCCTTGG CCATGAACCA 
GGACTGGTGC AGCTGGTGAA CTACTACAGA GGAGCAGACA AGCTTTGCAG AAAGGCATCG 
CTGGTCAAGC TAATCAAGAC CAGCCCGGAG CTGTCCGACT CCAGCAACTG GTTTCCAGAG 
TCCTACATCA TCTACCCCAC GAACCTCAAC ACCCCTGTAG CTCCGGCTTC CAACGGCATC 
AGTCACCTGA AGAGCAATCC CAAAACGGAT GAGCGGGAGG TTTTCTTGGC CTCCTACCAC 
GCCAAGAAGG AAAATGGAGA GGGAAGCGTG TGGATCGCCA AGTCTTCTGC TGGTGCTAAA 
GGAGCTGGTA TTCTGATCTC ACACGATGCA AATCAGCTGC TGGACTACAT TGACAATCAG 
GGACAGGTTC ATGTCATCCA GAAGTACCTG GAGAAACCCC TGCTGCTGGA ACCCGGTCAT 
CGCAAGTTTG ACATCAGGAG CTGGGTGCTG GTGGACCATC AGTATAACAT CTATTTGTAC 
CGGGAGGGCG TGCTGCGGAC CTCGTCGGAG CCCTACAACA GCGCGGACCT CCAGGACATG 
ACCAGCCACC TGACCAACCA CTGCATCCAG AAGGAGCACT CCCAGAACTA CGGCCGCTAC 
GAGGAGGGCA ACGAGATGTT CTTTGACGAG TTCAGGCTGT ACCTGCTGAA CACCCACAAC 
GTCACCATGG AGACAACCAT ATTACCTCAG ATCAAACACG TCATAAAGAG CTGTCTGTCC 
TGCATCGAGG CGGCGATCAG CACCAAGCAC CTCTCCTACC AGAGCTTCCA GCTCTTTGGG 
TTTGACTTCA TGGTCGACGA GAACTTCAAA GTGTGGCTCA TCGAGATCAA CGGCGCTCCA 
GCCTGCGCCC AGAAGCTCTA TCCAGAGTTG TGTCAAGGCA TCATCGACGT GGCCATCTCC 
AGTGTCTTCA CTCTCAACAG TGACTCCTCG TCTGCGTCTT CCTCGCCTTA CTCCTCCTCC 
CCTTCTTCCC TCTTCACCAC CAACTCCTGC TCCTCCCCGA AGCTGAGGGG GCCCCTGCAC 
GTGGGTCCTT TCACTCGCCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttl ( XM_012856777.2 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012712231.1 Fundulus heteroclitus tubulin tyrosine ligase (ttl), mRNA.

Price & Availability↑

CloneID	OFu105483
Clone ID Related Accession (Same CDS sequence)	XM_012856777.1 , XM_012856777.2
Accession Version	XM_012856777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	tubulin--tyrosine ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012225088.1) and transcript sequence (GCES01041050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 1, 2017 this sequence version replaced XM_012856777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 181 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGCTCTC CGGTTTACAC GTTCGTTAGC CGCGACGACA ACAGCACCAT TTATGCTGAA 
GTGTCTAAAA TGCTGCTGGC CACCGGCCAC TGGAAGCGGC TGAAAAGAGA CAATCCCCGG 
TTCAACCTGA TGCTCGGGGA GCGGAACCGG CTGCCATTCG GACGCCTTGG CCATGAACCA 
GGACTGGTGC AGCTGGTGAA CTACTACAGA GGAGCAGACA AGCTTTGCAG AAAGGCATCG 
CTGGTCAAGC TAATCAAGAC CAGCCCGGAG CTGTCCGACT CCAGCAACTG GTTTCCAGAG 
TCCTACATCA TCTACCCCAC GAACCTCAAC ACCCCTGTAG CTCCGGCTTC CAACGGCATC 
AGTCACCTGA AGAGCAATCC CAAAACGGAT GAGCGGGAGG TTTTCTTGGC CTCCTACCAC 
GCCAAGAAGG AAAATGGAGA GGGAAGCGTG TGGATCGCCA AGTCTTCTGC TGGTGCTAAA 
GGAGCTGGTA TTCTGATCTC ACACGATGCA AATCAGCTGC TGGACTACAT TGACAATCAG 
GGACAGGTTC ATGTCATCCA GAAGTACCTG GAGAAACCCC TGCTGCTGGA ACCCGGTCAT 
CGCAAGTTTG ACATCAGGAG CTGGGTGCTG GTGGACCATC AGTATAACAT CTATTTGTAC 
CGGGAGGGCG TGCTGCGGAC CTCGTCGGAG CCCTACAACA GCGCGGACCT CCAGGACATG 
ACCAGCCACC TGACCAACCA CTGCATCCAG AAGGAGCACT CCCAGAACTA CGGCCGCTAC 
GAGGAGGGCA ACGAGATGTT CTTTGACGAG TTCAGGCTGT ACCTGCTGAA CACCCACAAC 
GTCACCATGG AGACAACCAT ATTACCTCAG ATCAAACACG TCATAAAGAG CTGTCTGTCC 
TGCATCGAGG CGGCGATCAG CACCAAGCAC CTCTCCTACC AGAGCTTCCA GCTCTTTGGG 
TTTGACTTCA TGGTCGACGA GAACTTCAAA GTGTGGCTCA TCGAGATCAA CGGCGCTCCA 
GCCTGCGCCC AGAAGCTCTA TCCAGAGTTG TGTCAAGGCA TCATCGACGT GGCCATCTCC 
AGTGTCTTCA CTCTCAACAG TGACTCCTCG TCTGCGTCTT CCTCGCCTTA CTCCTCCTCC 
CCTTCTTCCC TCTTCACCAC CAACTCCTGC TCCTCCCCGA AGCTGAGGGG GCCCCTGCAC 
GTGGGTCCTT TCACTCGCCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012712231.1
CDS	454..1677
Translation	MSSPVYTFVSRDDNSTIYAEVSKMLLATGHWKRLKRDNPRFNLMLGERNRLPFGRLGHEPGLVQLVNYYRGADKLCRKASLVKLIKTSPELSDSSNWFPESYIIYPTNLNTPVAPASNGISHLKSNPKTDEREVFLASYHAKKENGEGSVWIAKSSAGAKGAGILISHDANQLLDYIDNQGQVHVIQKYLEKPLLLEPGHRKFDIRSWVLVDHQYNIYLYREGVLRTSSEPYNSADLQDMTSHLTNHCIQKEHSQNYGRYEEGNEMFFDEFRLYLLNTHNVTMETTILPQIKHVIKSCLSCIEAAISTKHLSYQSFQLFGFDFMVDENFKVWLIEINGAPACAQKLYPELCQGIIDVAISSVFTLNSDSSSASSSPYSSSPSSLFTTNSCSSPKLRGPLHVGPFTRL

Target ORF information:

RefSeq Version	XM_012856777.2
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus tubulin tyrosine ligase (ttl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012856777.2

ORF Insert Sequence:

ATGAGCTCTC CGGTTTACAC GTTCGTTAGC CGCGACGACA ACAGCACCAT TTATGCTGAA 
GTGTCTAAAA TGCTGCTGGC CACCGGCCAC TGGAAGCGGC TGAAAAGAGA CAATCCCCGG 
TTCAACCTGA TGCTCGGGGA GCGGAACCGG CTGCCATTCG GACGCCTTGG CCATGAACCA 
GGACTGGTGC AGCTGGTGAA CTACTACAGA GGAGCAGACA AGCTTTGCAG AAAGGCATCG 
CTGGTCAAGC TAATCAAGAC CAGCCCGGAG CTGTCCGACT CCAGCAACTG GTTTCCAGAG 
TCCTACATCA TCTACCCCAC GAACCTCAAC ACCCCTGTAG CTCCGGCTTC CAACGGCATC 
AGTCACCTGA AGAGCAATCC CAAAACGGAT GAGCGGGAGG TTTTCTTGGC CTCCTACCAC 
GCCAAGAAGG AAAATGGAGA GGGAAGCGTG TGGATCGCCA AGTCTTCTGC TGGTGCTAAA 
GGAGCTGGTA TTCTGATCTC ACACGATGCA AATCAGCTGC TGGACTACAT TGACAATCAG 
GGACAGGTTC ATGTCATCCA GAAGTACCTG GAGAAACCCC TGCTGCTGGA ACCCGGTCAT 
CGCAAGTTTG ACATCAGGAG CTGGGTGCTG GTGGACCATC AGTATAACAT CTATTTGTAC 
CGGGAGGGCG TGCTGCGGAC CTCGTCGGAG CCCTACAACA GCGCGGACCT CCAGGACATG 
ACCAGCCACC TGACCAACCA CTGCATCCAG AAGGAGCACT CCCAGAACTA CGGCCGCTAC 
GAGGAGGGCA ACGAGATGTT CTTTGACGAG TTCAGGCTGT ACCTGCTGAA CACCCACAAC 
GTCACCATGG AGACAACCAT ATTACCTCAG ATCAAACACG TCATAAAGAG CTGTCTGTCC 
TGCATCGAGG CGGCGATCAG CACCAAGCAC CTCTCCTACC AGAGCTTCCA GCTCTTTGGG 
TTTGACTTCA TGGTCGACGA GAACTTCAAA GTGTGGCTCA TCGAGATCAA CGGCGCTCCA 
GCCTGCGCCC AGAAGCTCTA TCCAGAGTTG TGTCAAGGCA TCATCGACGT GGCCATCTCC 
AGTGTCTTCA CTCTCAACAG TGACTCCTCG TCTGCGTCTT CCTCGCCTTA CTCCTCCTCC 
CCTTCTTCCC TCTTCACCAC CAACTCCTGC TCCTCCCCGA AGCTGAGGGG GCCCCTGCAC 
GTGGGTCCTT TCACTCGCCT CTAG