AYL99_07330 cDNA ORF clone, Fonsecaea erecta

Gene
Clones
gRNAs

Summary

Gene Symbol	AYL99_07330
Entrez Gene ID	30011498
Full Name	tRNA pseudouridine(38-40) synthase, variant
Gene Type	protein-coding
Organism	Fonsecaea erecta

mRNA and Protein(s)

mRNA	Protein	Name
XM_018838839.1	XP_018691607.1	tRNA pseudouridine(38-40) synthase, variant

Pathways from BioSystems
Gene Ontology

The following AYL99_07330 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AYL99_07330 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFo19667	XM_018838839.1 Latest version!	Fonsecaea erecta tRNA pseudouridine(38-40) synthase, variant (AYL99_07330), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AYL99_07330 ( XM_018838839.1 ) cDNA ORF clone, Fonsecaea erecta -> XP_018691607.1 Fonsecaea erecta tRNA pseudouridine(38-40) synthase, variant (AYL99_07330), partial mRNA.

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CloneID	OFo19667
Clone ID Related Accession (Same CDS sequence)	XM_018838839.1
Accession Version	XM_018838839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-27
Organism	Fonsecaea erecta
Product	tRNA pseudouridine(38-40) synthase, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_017387249). COMPLETENESS: incomplete on both ends.

ATGGAGACTG GCATTCCGCA GGCCCCCGCG CCGAGTGCGT CCCAGGGCGC CTCAACCTCC 
AATATCGACA CCGAATCGAG GACTCCCACC TCTCCCAAAG ACGGAGCAGC GCAAGTTCGT 
GAGCCAGGAC ATGGTGGTTC AGATGCAAGA CGTCCGCATC AGAATGCTCC TGGAGGGCGA 
GCGGCCCGAG GTGGTCGAGG AGGTCGTCAG GGCAAGAAGA GAGATATGGG GAGGAAGGAG 
TGGGCTAAAA CCAAAGTAGA CAAAAGGCAA CGGAACGAGG ATGAGCAAGC CGCTAAACGT 
CAACGGATCG ACCAAGGCAA GGATGCACTG CCCATTTACG CTACAAAATT CAGCGAGGAA 
GTGCTTGCCG CCGAGGAGAG GAGACCCAAA AAGAAGGTTG CTGTGCTCAT TGGGTACGCT 
GGTACCGGTT ACAAGGGGAT GCAGCTAGCA CACGACCAGA AAACTATCGA AGGAGACCTC 
TTCCAAGCCT TTGTCGCGGC CGGCGCCATC TCTAAGGCCA ACGCCGACGA TCCCAAGAAA 
TCTTCCTTTG TGCGATGCGC CCGGACCGAC AAGGGCGTCC ATGCCGCCGG CAACGTGATC 
TCCTTGAAAT TGATCATCGA GGACGAAGAC ATAGTGCAGA AGATCAACAA CAATCTGACT 
CCCCAGATCC GGGTCTGGGG CTACGAACGG ACAAGTAACA GTTTCAGCGC CTACCAGATG 
GTCGACTCGC GCATATATGA GTACCTGATC CCGACACATA GTTTTCTACC ACCCCACCCT 
AGCAGTTTTT TGGGGAGAAA CTGCGAGAAG TGGGCAGAAA AAACGGACGA CGTGGACGGA 
TACAGAAAGA GACAAGAGGA AGTAACAGGG TACTGGGAGA AGGTGGACGA GGAGATGATC 
AAGCCGATTC TGGCCGAGTA TGACGAAGAG ATCCGTGCCA TTTTGGAAAA AGCACTCTGG 
CTGAAAACAG AACGCCCGAC GGATGACGGG AATAGCGGTG CTCCCCCGCC GAATCCCCCA 
GTCATGGAGG TTGAACCTGA AACTTCTAAG CCAGAGGCTC CACAAGAAGC CGAGGACAAG 
GACCGTGCTC CCGAGAAGAG CAACAACGAA GAACCCGCCT CCGATGCAGG AGACGCAACA 
GAAGAGCCCA AGTTATCCCG ATCGCGTGTC CTCGAGGAAG CAATGAAACG CGTCCGGCAA 
GCCTATATCG CTGCCAAACG GGCCTATCGC ATTCCTCCTC AACGTCTCGA CCGAATTCAA 
GAAGCGCTCA GTCTATTTGT CGGCACTCGC AACTATCACA ACTACACTAT CCAGAAGACC 
TTCAAGGACC CCAGCGCCAA ACGAGTGATC AAGTCCTTCC TGGTCAACAG GACGCCTATT 
CTTATCAATG GAACGGAATG GCTCAGTATG AAAGTCCACG GGCAGAGTTT CATGATGCAC 
CAGATCCGCA AGATGGTTGG GATGGTTGCG CTCGTGGTGC GCTGCGGATG TGATCCCAAA 
CGCCTGGTGG AAAGTATGGG TCCTGACAGG CTGTCCATCC CCAAGGCTCC TAGCCTGGGT 
CTTCTGCTCG AACGGCCGGT GTTTGAATCT TACAACAGGC GCGCCAAAAG CGAACTCAGT 
AAAGAAGCCA TCGATTTTGA CAAGTACCGG GAGGAGATGG ACCAGTTCAA GCAAGTACAG 
ATCTACGAGC GCATGTATCG AGACGAGGAG AAGGACAACG TCTTTGGCAA CTTCTTCAAC 
CACGTCGACA ACTTTCCGAG TGAGATTTTC TTGTTTGTGA CCTCGGGCGG CATCCAAGCC 
ACCAAGACGA AAGCACTGGT TGGGGCAGTC GAGGTTGTCA AGACGAAGAG TGGAGAAGAT 
GCTCTATTGA GCAACGATGT CGACGTCGGC GTCGAGGACG AGGACAGCGA TGCGGACGAC 
GCTGTCAAGG ATAACCACGG TGACGAGGGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018691607.1
CDS	1..1953
Translation	METGIPQAPAPSASQGASTSNIDTESRTPTSPKDGAAQVREPGHGGSDARRPHQNAPGGRAARGGRGGRQGKKRDMGRKEWAKTKVDKRQRNEDEQAAKRQRIDQGKDALPIYATKFSEEVLAAEERRPKKKVAVLIGYAGTGYKGMQLAHDQKTIEGDLFQAFVAAGAISKANADDPKKSSFVRCARTDKGVHAAGNVISLKLIIEDEDIVQKINNNLTPQIRVWGYERTSNSFSAYQMVDSRIYEYLIPTHSFLPPHPSSFLGRNCEKWAEKTDDVDGYRKRQEEVTGYWEKVDEEMIKPILAEYDEEIRAILEKALWLKTERPTDDGNSGAPPPNPPVMEVEPETSKPEAPQEAEDKDRAPEKSNNEEPASDAGDATEEPKLSRSRVLEEAMKRVRQAYIAAKRAYRIPPQRLDRIQEALSLFVGTRNYHNYTIQKTFKDPSAKRVIKSFLVNRTPILINGTEWLSMKVHGQSFMMHQIRKMVGMVALVVRCGCDPKRLVESMGPDRLSIPKAPSLGLLLERPVFESYNRRAKSELSKEAIDFDKYREEMDQFKQVQIYERMYRDEEKDNVFGNFFNHVDNFPSEIFLFVTSGGIQATKTKALVGAVEVVKTKSGEDALLSNDVDVGVEDEDSDADDAVKDNHGDEG

Target ORF information:

RefSeq Version	XM_018838839.1
Organism	Fonsecaea erecta
Definition	Fonsecaea erecta tRNA pseudouridine(38-40) synthase, variant (AYL99_07330), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018838839.1

ORF Insert Sequence:

ATGGAGACTG GCATTCCGCA GGCCCCCGCG CCGAGTGCGT CCCAGGGCGC CTCAACCTCC 
AATATCGACA CCGAATCGAG GACTCCCACC TCTCCCAAAG ACGGAGCAGC GCAAGTTCGT 
GAGCCAGGAC ATGGTGGTTC AGATGCAAGA CGTCCGCATC AGAATGCTCC TGGAGGGCGA 
GCGGCCCGAG GTGGTCGAGG AGGTCGTCAG GGCAAGAAGA GAGATATGGG GAGGAAGGAG 
TGGGCTAAAA CCAAAGTAGA CAAAAGGCAA CGGAACGAGG ATGAGCAAGC CGCTAAACGT 
CAACGGATCG ACCAAGGCAA GGATGCACTG CCCATTTACG CTACAAAATT CAGCGAGGAA 
GTGCTTGCCG CCGAGGAGAG GAGACCCAAA AAGAAGGTTG CTGTGCTCAT TGGGTACGCT 
GGTACCGGTT ACAAGGGGAT GCAGCTAGCA CACGACCAGA AAACTATCGA AGGAGACCTC 
TTCCAAGCCT TTGTCGCGGC CGGCGCCATC TCTAAGGCCA ACGCCGACGA TCCCAAGAAA 
TCTTCCTTTG TGCGATGCGC CCGGACCGAC AAGGGCGTCC ATGCCGCCGG CAACGTGATC 
TCCTTGAAAT TGATCATCGA GGACGAAGAC ATAGTGCAGA AGATCAACAA CAATCTGACT 
CCCCAGATCC GGGTCTGGGG CTACGAACGG ACAAGTAACA GTTTCAGCGC CTACCAGATG 
GTCGACTCGC GCATATATGA GTACCTGATC CCGACACATA GTTTTCTACC ACCCCACCCT 
AGCAGTTTTT TGGGGAGAAA CTGCGAGAAG TGGGCAGAAA AAACGGACGA CGTGGACGGA 
TACAGAAAGA GACAAGAGGA AGTAACAGGG TACTGGGAGA AGGTGGACGA GGAGATGATC 
AAGCCGATTC TGGCCGAGTA TGACGAAGAG ATCCGTGCCA TTTTGGAAAA AGCACTCTGG 
CTGAAAACAG AACGCCCGAC GGATGACGGG AATAGCGGTG CTCCCCCGCC GAATCCCCCA 
GTCATGGAGG TTGAACCTGA AACTTCTAAG CCAGAGGCTC CACAAGAAGC CGAGGACAAG 
GACCGTGCTC CCGAGAAGAG CAACAACGAA GAACCCGCCT CCGATGCAGG AGACGCAACA 
GAAGAGCCCA AGTTATCCCG ATCGCGTGTC CTCGAGGAAG CAATGAAACG CGTCCGGCAA 
GCCTATATCG CTGCCAAACG GGCCTATCGC ATTCCTCCTC AACGTCTCGA CCGAATTCAA 
GAAGCGCTCA GTCTATTTGT CGGCACTCGC AACTATCACA ACTACACTAT CCAGAAGACC 
TTCAAGGACC CCAGCGCCAA ACGAGTGATC AAGTCCTTCC TGGTCAACAG GACGCCTATT 
CTTATCAATG GAACGGAATG GCTCAGTATG AAAGTCCACG GGCAGAGTTT CATGATGCAC 
CAGATCCGCA AGATGGTTGG GATGGTTGCG CTCGTGGTGC GCTGCGGATG TGATCCCAAA 
CGCCTGGTGG AAAGTATGGG TCCTGACAGG CTGTCCATCC CCAAGGCTCC TAGCCTGGGT 
CTTCTGCTCG AACGGCCGGT GTTTGAATCT TACAACAGGC GCGCCAAAAG CGAACTCAGT 
AAAGAAGCCA TCGATTTTGA CAAGTACCGG GAGGAGATGG ACCAGTTCAA GCAAGTACAG 
ATCTACGAGC GCATGTATCG AGACGAGGAG AAGGACAACG TCTTTGGCAA CTTCTTCAAC 
CACGTCGACA ACTTTCCGAG TGAGATTTTC TTGTTTGTGA CCTCGGGCGG CATCCAAGCC 
ACCAAGACGA AAGCACTGGT TGGGGCAGTC GAGGTTGTCA AGACGAAGAG TGGAGAAGAT 
GCTCTATTGA GCAACGATGT CGACGTCGGC GTCGAGGACG AGGACAGCGA TGCGGACGAC 
GCTGTCAAGG ATAACCACGG TGACGAGGGT TGA