PHKA1 cDNA ORF clone, Ficedula albicollis(collared flycatcher)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHKA1
Entrez Gene ID	101806879
Full Name	phosphorylase kinase regulatory subunit alpha 1
Gene Type	protein-coding
Organism	Ficedula albicollis(collared flycatcher)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005046230.2	XP_005046287.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform

Pathways from BioSystems
Gene Ontology

The following PHKA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHKA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFi16086	XM_005046230.2 Latest version!	Ficedula albicollis phosphorylase kinase, alpha 1 (muscle) (PHKA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHKA1 ( XM_005046230.2 ) cDNA ORF clone, Ficedula albicollis(collared flycatcher) -> XP_005046287.1 Ficedula albicollis phosphorylase kinase, alpha 1 (muscle) (PHKA1), mRNA.

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CloneID	OFi16086
Clone ID Related Accession (Same CDS sequence)	XM_005046230.2
Accession Version	XM_005046230.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-19
Organism	Ficedula albicollis(collared flycatcher)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_021676.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 20, 2016 this sequence version replaced XM_005046230.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ficedula albicollis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GGAGCAACTC GGGCGTGCGT CTGGACGGCT ACGGGCGCCT GGTGCAGCAG 
ACTATCCTCC GCCACCAGGA CGCGGTGACG GGGCTGCTCC CCGCCAGCGC CGACTACCAG 
GACGCCTGGG TCCGGGACAA CGTGTACAGC ATCCTGGCGG TGTGGGGCCT TGGCCTGGCC 
TACCGCAAGA ATGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGCGAGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTAGAG 
GCCTTCAAGT ACAGTCAGAG CACCAGGGAC TGTCTGCATG CCAAGTATAA CACCCACACC 
TGTGCCACTG TGGTAGGAGA CCACGAGTGG GGTCACCTAC AGATGGATGC TACCTCCCTC 
TACCTCCTCA TGCTTGCACA AATGACGGCC TCAGGCCTCC ACATAATTCA CAGCTTGGAT 
GAAGTCAATT TTATCCAGAA CCTTGTATTC TACATTGAAG CTGCCTACAA AACTGCGGAC 
TTTGGCATAT GGGAGCGTGG GGACAAGACA AACCAGGGCA TCACAGAGTT GAATGCCAGT 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCACTGGATG AGCTGGATCT CTTTGGAGCC 
AAGGGAGGCC CACAGTCAGT GATACGGGTG CTGTCCGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CAATGCTGCC CAGGGCCTCC ACATCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCGGC ATTTGCTGTG GAGGACAGTG AGCTGGTGGA AATAACCAAG 
CAGGAGATTA TCACCAAGCT GCAGGGGCGG TATGGCTGTT GCCGGTTCCT CCGGGATGGA 
TACAGGACTC CCAAAGAGGA CCCCAAACGC CTCTACTACG AACCTGCTGA GTTGAAGCTG 
TTTGAGAACA TTGAGTGTGA GTGGCCTCTC TTCTGGGCAT ACCTGATGAT TGATGGGATT 
TTCAGTGGAA ACATGGAGCA GGTGCAGGAG TACCGGGAAG CCCTCGAGGG GGTTCTCATC 
AAGGGGAAGA ATGGGGTACG CCTGCTGCCA GAGCTGTACA GTGTCCCACC AGATAAGGTG 
GATGAAGAAT ACAGGAATCC ACACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACAC 
ATGTGGGGAC AGTCCCTCTA CATCCTGGGC TGTCTGATGG CTGAGGGGTT TCTAGCTCCA 
GGTGAAATAG ATCCCCTCAA CCGCCGTTTC GCGACTGTCC CTAAACCTGA TGTGGTGGTC 
CAAGTGTGTA TCCTGGCTGA AACAGAGGGG ATCAAGGCAA TCCTGCAGAA GGAGGGCATT 
GACGTGGAAA CTGTGGCGGA CGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTATG CCCGGCTGGG CCGCAACAAG CAGATGTGCC TTACTGGACG GCCCTACCGG 
CATATGGGTG TCCTTGGGAC CTCCAAGCTC TACAAAATCA GGAAGAGCAT CTTTACCTTT 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TTGACAACAA GATGATTGTG 
GAGATGCTGA GGACGGACCT CTCCTACCTC TGCAGCCGCT GGAGGATGAC TGGGCGACCA 
ACCATCACCT TCCCCATCTC CCACACCATG CTCGACGAAA CCAGCAGCAG TGTGCATCCC 
ACGGTGCTGG CAATGCTGCA GAAGCTGCAG GATGGGTATT TTGGTGGTGC AAGAATTCAG 
ACTGGTAAAT TGTCGGAGTT CCTGACAACA TCGTGCCGAA CGCACCTCAG CTTTATGGAC 
CCTGGGCCAG AGGAGGACAC TGATGATGAG GTTGCTCAGT ATCTGGATCA CTTGCTGCAG 
CACACGACTC CCCAGTCAAA CCTGCCTCCC ACCACCCAGC GGGGAGGGCT GAGCCGCTTC 
AGGGCTGCTG TCCACACCAC CCGTGACCTC ATGTCCCTGG CATCCAAGGC CAAGGACCTG 
CATGTCCAGA ATGTTGGGAT GTATGTCCCC AGCAGGATCT TCCAGGCATC GCAGCAGTCG 
GTCAAGCTGC TGAGTTCACC CCACCAGCAT GACTTGGATA GCAAGAGCCA CGCACTCTAC 
GCAGAGATGA ACTTGCCACG TGATGAGGAT GGCAACGTGG ACTGCAAGGC ATTGGTGGAC 
CAGTTGCGCC TCTGCCCCAC GCTGCAGGAG CAAGCAGACA TCCTTTACAT GCTGCACATC 
CTCAAGGGAC CTGAGTGGCA CACAGGGCTC GATAGTGAGC CTGGCCCCAC CGTCAGAGAG 
CTCCTCACTG AGCTGTATGT GCGGGTGGGG GAGACACGCC AGTGGGCCCT GATCCGCTAC 
ATCTCTGGCA TCCTCAAGAA GAAGGTTGAA GCTCTGGATG AGGCCTGTAC TGACCTCCTG 
TCTCACCAGA AACACTTGAC AGTGGGGCTG CCCCCAGAGC CACGTGAGAA GACAATCTCC 
ACCCCGATCC CCTATGAGGA ACTCGTTCGC CTTATTGATG AAGCCAGTGA GAAGAACCTC 
AGTGTGTCCG TCCTCACCCA GGAGATCATG GTGTACTTGG CCATGTACAT ACGCACACAG 
CCTGCGCTGT TTGCCGAGAT GTTCCGCATC CGCATTGGGC TCATCATCCA GGTGATGGCA 
ACAGAGCTGG CACACTCCCT GCAGTGTTCA GCTGAAGAAG CCACAGAGAA CCTGATGAAT 
CTCAGCCCTT CAGACATGAA GAGCCTCCTC TACCACATCC TCTCTGGCAA GGAGTTTGGG 
GTGGAAAAGA GCGTGCGTTC AGTGGACTCG TCGCTCACCA CTGCTGTCTC CATCTGTGAC 
ATGGAAGCTG TCGGGGCCTC CAGGCCTGAG CGCACTGGCC TTGTCAGGCT GAAAAGTGAA 
ATCAAACAGC AATTGGATAA ACGTAGGCAG AGTCTGCCTG GGAGTAAGCC GCTCAGTTTG 
CAGTCCAGGG ACACCGACCT CAAGTCCTTT CTGTCTGCTG GGCACACAGA GCTGCTGGGC 
TCCTTTTCAA GCATGCTAGA AGACCAGGGC AGTAAGGACA GCCGGCAGGG CCAGTGGCAG 
CGGAGGCGCC GGCTGGATGG GGCCCTTAAC CGTGTGCCTG TGGGCTTCTA TCAGAAGGTC 
TGGAAGGTCC TGCAGAAGTG CCATGGCCTC TCTGTGGAGG GCTTTGTTCT TCCCTCCTCG 
ACAACCAGAG AGATGACCCC AGGGGAAATG AAGTTTGCTG TGCATGTGGA GTCTGTCCTC 
AACCGTGTGC CCCAGCCAGA GTACAGGCAG CTCCTGGTGG AAGCTATCTT AGTGCTCACC 
ATGCTTGTGG ACATGGAGGT GCACACCATT GGTGGCATCA TCGCTGTGGA AAAGATCTTG 
CACATAGCCA ATGACCTCTT CTATGAGGAG CAGAAAGCTC TGGGCGCTGA TGAGCACATG 
CTGGAGAGGG ATCCTTCGAC AGGCATCTGC AGCCTGCTGT ATGACAGTGC ACCGAGTGGC 
CGGTTTGGCA CCATGACCTA CCTGTCCAAG TCGGTGGCCT TGTACGTGTA CGACTTTCTG 
CCCACAGATG GCTGCTCCAT GCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005046287.1
CDS	69..3695
Translation	MRSRSNSGVRLDGYGRLVQQTILRHQDAVTGLLPASADYQDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMMRQVDKVEAFKYSQSTRDCLHAKYNTHTCATVVGDHEWGHLQMDATSLYLLMLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGITELNASSVGMAKAALEALDELDLFGAKGGPQSVIRVLSDEVQHCQSILHSMLPRASTSKEVDASVLSVISYPAFAVEDSELVEITKQEIITKLQGRYGCCRFLRDGYRTPKEDPKRLYYEPAELKLFENIECEWPLFWAYLMIDGIFSGNMEQVQEYREALEGVLIKGKNGVRLLPELYSVPPDKVDEEYRNPHTVDRIPMGKLPHMWGQSLYILGCLMAEGFLAPGEIDPLNRRFATVPKPDVVVQVCILAETEGIKAILQKEGIDVETVADVYPIRVQPARILSHIYARLGRNKQMCLTGRPYRHMGVLGTSKLYKIRKSIFTFTPQFIDQQQFYLALDNKMIVEMLRTDLSYLCSRWRMTGRPTITFPISHTMLDETSSSVHPTVLAMLQKLQDGYFGGARIQTGKLSEFLTTSCRTHLSFMDPGPEEDTDDEVAQYLDHLLQHTTPQSNLPPTTQRGGLSRFRAAVHTTRDLMSLASKAKDLHVQNVGMYVPSRIFQASQQSVKLLSSPHQHDLDSKSHALYAEMNLPRDEDGNVDCKALVDQLRLCPTLQEQADILYMLHILKGPEWHTGLDSEPGPTVRELLTELYVRVGETRQWALIRYISGILKKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISTPIPYEELVRLIDEASEKNLSVSVLTQEIMVYLAMYIRTQPALFAEMFRIRIGLIIQVMATELAHSLQCSAEEATENLMNLSPSDMKSLLYHILSGKEFGVEKSVRSVDSSLTTAVSICDMEAVGASRPERTGLVRLKSEIKQQLDKRRQSLPGSKPLSLQSRDTDLKSFLSAGHTELLGSFSSMLEDQGSKDSRQGQWQRRRRLDGALNRVPVGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEMKFAVHVESVLNRVPQPEYRQLLVEAILVLTMLVDMEVHTIGGIIAVEKILHIANDLFYEEQKALGADEHMLERDPSTGICSLLYDSAPSGRFGTMTYLSKSVALYVYDFLPTDGCSMQ

Target ORF information:

RefSeq Version	XM_005046230.2
Organism	Ficedula albicollis(collared flycatcher)
Definition	Ficedula albicollis phosphorylase kinase, alpha 1 (muscle) (PHKA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005046230.2

ORF Insert Sequence:

ATGAGGAGCC GGAGCAACTC GGGCGTGCGT CTGGACGGCT ACGGGCGCCT GGTGCAGCAG 
ACTATCCTCC GCCACCAGGA CGCGGTGACG GGGCTGCTCC CCGCCAGCGC CGACTACCAG 
GACGCCTGGG TCCGGGACAA CGTGTACAGC ATCCTGGCGG TGTGGGGCCT TGGCCTGGCC 
TACCGCAAGA ATGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGCGAGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTAGAG 
GCCTTCAAGT ACAGTCAGAG CACCAGGGAC TGTCTGCATG CCAAGTATAA CACCCACACC 
TGTGCCACTG TGGTAGGAGA CCACGAGTGG GGTCACCTAC AGATGGATGC TACCTCCCTC 
TACCTCCTCA TGCTTGCACA AATGACGGCC TCAGGCCTCC ACATAATTCA CAGCTTGGAT 
GAAGTCAATT TTATCCAGAA CCTTGTATTC TACATTGAAG CTGCCTACAA AACTGCGGAC 
TTTGGCATAT GGGAGCGTGG GGACAAGACA AACCAGGGCA TCACAGAGTT GAATGCCAGT 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCACTGGATG AGCTGGATCT CTTTGGAGCC 
AAGGGAGGCC CACAGTCAGT GATACGGGTG CTGTCCGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CAATGCTGCC CAGGGCCTCC ACATCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCGGC ATTTGCTGTG GAGGACAGTG AGCTGGTGGA AATAACCAAG 
CAGGAGATTA TCACCAAGCT GCAGGGGCGG TATGGCTGTT GCCGGTTCCT CCGGGATGGA 
TACAGGACTC CCAAAGAGGA CCCCAAACGC CTCTACTACG AACCTGCTGA GTTGAAGCTG 
TTTGAGAACA TTGAGTGTGA GTGGCCTCTC TTCTGGGCAT ACCTGATGAT TGATGGGATT 
TTCAGTGGAA ACATGGAGCA GGTGCAGGAG TACCGGGAAG CCCTCGAGGG GGTTCTCATC 
AAGGGGAAGA ATGGGGTACG CCTGCTGCCA GAGCTGTACA GTGTCCCACC AGATAAGGTG 
GATGAAGAAT ACAGGAATCC ACACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACAC 
ATGTGGGGAC AGTCCCTCTA CATCCTGGGC TGTCTGATGG CTGAGGGGTT TCTAGCTCCA 
GGTGAAATAG ATCCCCTCAA CCGCCGTTTC GCGACTGTCC CTAAACCTGA TGTGGTGGTC 
CAAGTGTGTA TCCTGGCTGA AACAGAGGGG ATCAAGGCAA TCCTGCAGAA GGAGGGCATT 
GACGTGGAAA CTGTGGCGGA CGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTATG CCCGGCTGGG CCGCAACAAG CAGATGTGCC TTACTGGACG GCCCTACCGG 
CATATGGGTG TCCTTGGGAC CTCCAAGCTC TACAAAATCA GGAAGAGCAT CTTTACCTTT 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TTGACAACAA GATGATTGTG 
GAGATGCTGA GGACGGACCT CTCCTACCTC TGCAGCCGCT GGAGGATGAC TGGGCGACCA 
ACCATCACCT TCCCCATCTC CCACACCATG CTCGACGAAA CCAGCAGCAG TGTGCATCCC 
ACGGTGCTGG CAATGCTGCA GAAGCTGCAG GATGGGTATT TTGGTGGTGC AAGAATTCAG 
ACTGGTAAAT TGTCGGAGTT CCTGACAACA TCGTGCCGAA CGCACCTCAG CTTTATGGAC 
CCTGGGCCAG AGGAGGACAC TGATGATGAG GTTGCTCAGT ATCTGGATCA CTTGCTGCAG 
CACACGACTC CCCAGTCAAA CCTGCCTCCC ACCACCCAGC GGGGAGGGCT GAGCCGCTTC 
AGGGCTGCTG TCCACACCAC CCGTGACCTC ATGTCCCTGG CATCCAAGGC CAAGGACCTG 
CATGTCCAGA ATGTTGGGAT GTATGTCCCC AGCAGGATCT TCCAGGCATC GCAGCAGTCG 
GTCAAGCTGC TGAGTTCACC CCACCAGCAT GACTTGGATA GCAAGAGCCA CGCACTCTAC 
GCAGAGATGA ACTTGCCACG TGATGAGGAT GGCAACGTGG ACTGCAAGGC ATTGGTGGAC 
CAGTTGCGCC TCTGCCCCAC GCTGCAGGAG CAAGCAGACA TCCTTTACAT GCTGCACATC 
CTCAAGGGAC CTGAGTGGCA CACAGGGCTC GATAGTGAGC CTGGCCCCAC CGTCAGAGAG 
CTCCTCACTG AGCTGTATGT GCGGGTGGGG GAGACACGCC AGTGGGCCCT GATCCGCTAC 
ATCTCTGGCA TCCTCAAGAA GAAGGTTGAA GCTCTGGATG AGGCCTGTAC TGACCTCCTG 
TCTCACCAGA AACACTTGAC AGTGGGGCTG CCCCCAGAGC CACGTGAGAA GACAATCTCC 
ACCCCGATCC CCTATGAGGA ACTCGTTCGC CTTATTGATG AAGCCAGTGA GAAGAACCTC 
AGTGTGTCCG TCCTCACCCA GGAGATCATG GTGTACTTGG CCATGTACAT ACGCACACAG 
CCTGCGCTGT TTGCCGAGAT GTTCCGCATC CGCATTGGGC TCATCATCCA GGTGATGGCA 
ACAGAGCTGG CACACTCCCT GCAGTGTTCA GCTGAAGAAG CCACAGAGAA CCTGATGAAT 
CTCAGCCCTT CAGACATGAA GAGCCTCCTC TACCACATCC TCTCTGGCAA GGAGTTTGGG 
GTGGAAAAGA GCGTGCGTTC AGTGGACTCG TCGCTCACCA CTGCTGTCTC CATCTGTGAC 
ATGGAAGCTG TCGGGGCCTC CAGGCCTGAG CGCACTGGCC TTGTCAGGCT GAAAAGTGAA 
ATCAAACAGC AATTGGATAA ACGTAGGCAG AGTCTGCCTG GGAGTAAGCC GCTCAGTTTG 
CAGTCCAGGG ACACCGACCT CAAGTCCTTT CTGTCTGCTG GGCACACAGA GCTGCTGGGC 
TCCTTTTCAA GCATGCTAGA AGACCAGGGC AGTAAGGACA GCCGGCAGGG CCAGTGGCAG 
CGGAGGCGCC GGCTGGATGG GGCCCTTAAC CGTGTGCCTG TGGGCTTCTA TCAGAAGGTC 
TGGAAGGTCC TGCAGAAGTG CCATGGCCTC TCTGTGGAGG GCTTTGTTCT TCCCTCCTCG 
ACAACCAGAG AGATGACCCC AGGGGAAATG AAGTTTGCTG TGCATGTGGA GTCTGTCCTC 
AACCGTGTGC CCCAGCCAGA GTACAGGCAG CTCCTGGTGG AAGCTATCTT AGTGCTCACC 
ATGCTTGTGG ACATGGAGGT GCACACCATT GGTGGCATCA TCGCTGTGGA AAAGATCTTG 
CACATAGCCA ATGACCTCTT CTATGAGGAG CAGAAAGCTC TGGGCGCTGA TGAGCACATG 
CTGGAGAGGG ATCCTTCGAC AGGCATCTGC AGCCTGCTGT ATGACAGTGC ACCGAGTGGC 
CGGTTTGGCA CCATGACCTA CCTGTCCAAG TCGGTGGCCT TGTACGTGTA CGACTTTCTG 
CCCACAGATG GCTGCTCCAT GCAGTAG